CN106959326A - Multi-parameter based on saliva property quantitatively detects ovulation device - Google Patents
Multi-parameter based on saliva property quantitatively detects ovulation device Download PDFInfo
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- 210000003296 saliva Anatomy 0.000 title claims abstract description 85
- 230000016087 ovulation Effects 0.000 title claims abstract description 75
- 238000012545 processing Methods 0.000 claims abstract description 31
- 230000005540 biological transmission Effects 0.000 claims abstract description 13
- 238000007619 statistical method Methods 0.000 claims abstract description 6
- 230000005518 electrochemistry Effects 0.000 claims abstract description 5
- 238000012546 transfer Methods 0.000 claims abstract description 4
- 238000001514 detection method Methods 0.000 claims description 37
- 238000000034 method Methods 0.000 claims description 13
- 102000009151 Luteinizing Hormone Human genes 0.000 claims description 7
- 108010073521 Luteinizing Hormone Proteins 0.000 claims description 7
- 210000002394 ovarian follicle Anatomy 0.000 claims description 6
- 230000008569 process Effects 0.000 claims description 6
- 229940040129 luteinizing hormone Drugs 0.000 claims description 5
- 238000002347 injection Methods 0.000 claims description 4
- 239000007924 injection Substances 0.000 claims description 4
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 3
- 239000010931 gold Substances 0.000 claims description 3
- 229910052737 gold Inorganic materials 0.000 claims description 3
- JUWSSMXCCAMYGX-UHFFFAOYSA-N gold platinum Chemical compound [Pt].[Au] JUWSSMXCCAMYGX-UHFFFAOYSA-N 0.000 claims description 3
- 241001062009 Indigofera Species 0.000 claims 1
- 239000007788 liquid Substances 0.000 claims 1
- 238000012360 testing method Methods 0.000 abstract description 22
- 102000012673 Follicle Stimulating Hormone Human genes 0.000 description 19
- 108010079345 Follicle Stimulating Hormone Proteins 0.000 description 19
- 229940028334 follicle stimulating hormone Drugs 0.000 description 19
- 230000008859 change Effects 0.000 description 6
- 238000010586 diagram Methods 0.000 description 6
- 238000012544 monitoring process Methods 0.000 description 6
- 230000036760 body temperature Effects 0.000 description 5
- 229940011871 estrogen Drugs 0.000 description 5
- 239000000262 estrogen Substances 0.000 description 5
- 102000002322 Egg Proteins Human genes 0.000 description 4
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- 238000002604 ultrasonography Methods 0.000 description 3
- 239000000579 Gonadotropin-Releasing Hormone Substances 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 210000004246 corpus luteum Anatomy 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000003325 follicular Effects 0.000 description 2
- XLXSAKCOAKORKW-AQJXLSMYSA-N gonadorelin Chemical compound C([C@@H](C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N1[C@@H](CCC1)C(=O)NCC(N)=O)NC(=O)[C@H](CO)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@H](CC=1N=CNC=1)NC(=O)[C@H]1NC(=O)CC1)C1=CC=C(O)C=C1 XLXSAKCOAKORKW-AQJXLSMYSA-N 0.000 description 2
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- NMJREATYWWNIKX-UHFFFAOYSA-N GnRH Chemical compound C1CCC(C(=O)NCC(N)=O)N1C(=O)C(CC(C)C)NC(=O)C(CC=1C2=CC=CC=C2NC=1)NC(=O)CNC(=O)C(NC(=O)C(CO)NC(=O)C(CC=1C2=CC=CC=C2NC=1)NC(=O)C(CC=1NC=NC=1)NC(=O)C1NC(=O)CC1)CC1=CC=C(O)C=C1 NMJREATYWWNIKX-UHFFFAOYSA-N 0.000 description 1
- 244000283207 Indigofera tinctoria Species 0.000 description 1
- 101800003527 Ovulation hormone Proteins 0.000 description 1
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- 230000005856 abnormality Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
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- 238000003745 diagnosis Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000003487 electrochemical reaction Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000004438 eyesight Effects 0.000 description 1
- 230000009123 feedback regulation Effects 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 230000003054 hormonal effect Effects 0.000 description 1
- 210000003016 hypothalamus Anatomy 0.000 description 1
- 238000001453 impedance spectrum Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/27—Association of two or more measuring systems or cells, each measuring a different parameter, where the measurement results may be either used independently, the systems or cells being physically associated, or combined to produce a value for a further parameter
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- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
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- General Health & Medical Sciences (AREA)
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Abstract
Ovulation device is quantitatively detected the invention provides a kind of multi-parameter based on saliva property, including：Saliva collecting device, for gathering saliva, including micro fluidic device；Electrochemical data acquisition module, electric current, current potential and impedance signal for gathering saliva electrochemistry, including acquisition electrode and signal processing circuit；Data transmission module, for the data transfer that gathers electrochemical data acquisition module to data processing module；Data processing module, for carrying out data processing and statistical analysis to the data received；And power module, for being powered to electrochemical data acquisition module and data transmission module.The multi-parameter based on saliva property that the present invention is provided quantitatively detects ovulation device, solves the problem of prior art ovulation tests time accuracy is not good.
Description
Technical field
The present invention relates to vitro detection technical field, especially, it is related to a kind of multi-parameter based on saliva property and quantitatively examines
Survey ovulation device.
Background technology
From the point of view of current prior art, the detailed mechanism and details of human ovulation are also not very clear.But around ovulation, body
Interior FSH (FSH), lutropin (LH), estrogen and the gonadotropin-releasing hormone (GRH) secreted by hypothalamus
Deng the feedback regulation reaction formed between hormone, the change for causing preovulatory hormonal readiness regular and fierce is that ovulation is completed
And the inducement that corpus luteum is formed after ovulation.Have been observed that generation of three hormone peak values to ovulation plays an important role：First
Estrogen peak value occurs, induction GuRH-A (GnRH) release increase, thus it is several to trigger LH, FSH
It is synchronous to reach secretion peak value.During induced ovulation occurs, FSH and LH must act synergistically, only a certain proportion of FSH
Cooperate with ability effectively induced ovulation mutually with LH, when LH induction ovulations are used alone, the ovarian follicle that ovary has been raised will be whole broken
When splitting, and using a certain proportion of FSH and LH cooperation induction ovulations, only ripe ovarian follicle just ruptures ovulation.
Monitoring ovary ovulation can be used to lower indirect method and be monitored：
(1) basal body temperature monitoring：Basal body temperature refers to body temperature when early morning is inactive, relatively low always in preovulatory phase, row
Substantially increase about 0.2~0.5 DEG C after ovum, be continued until menstruation.Though easy to be economical, accuracy is poor.
(2) Ovulation prediction is monitored：In the first half of menstrual cycle, uterine neck does not secrete mucus, and vulva is also very dry.Hereafter
The a small amount of sticky and opaque mucus of uterine neck secretion.Before will occurring to ovulation, with the appearance on estrogen peak, Ovulation prediction becomes
Obtain thin, transparent, limpid, amount also increases, referred to as fertility type mucus.Troublesome poeration.
(3) test paper method：Selling on the market has the test paper surveyed and ovulated, and can buy self testing.Test paper is placed on oneself
In urine, if two red lines occurs in " wicket " of test paper, explanation will ovulate.This method needs continuous several times to detect.But
Disturbing factor is more, can qualitative or Quasi-quantitative measurement.
(4) ultrasonoscopy monitoring (B ultrasound)
It is general to start monitoring the 10th day menstrual cycle, the change of follicular diameter is observed, in the follicular diameter of preovulatory 4 days
Average about to increase 3mm daily, in preovulatory follicle maturation about 17~25mm, ovarian follicle disappears after ovulation, and continuous monitoring is visible in ovulation
Preceding ovarian follicle is constantly grown up, and when maximum ovarian follicle disappears, points out to ovulate.The method is reliable, but needs continuous monitoring, expense
Height, it is time-consuming.
In addition to above-mentioned detection method, prior art also discloses the device that ovulation tests are carried out based on saliva.Scientific research
It was found that, the physical chemistry of onset of ovulation saliva can be influenceed and be changed by estrogen, salt content and some enzyme materials
Also in change.It can be seen that by ultramicroscopic observation, the crystal habit of saliva and the crystal habit of its cervical mucus extremely phase
Seemingly, the pattern of a kind of similar fern or plant leaf blade channels and collaterals is showed, this fernlike crystal image is general in preovulatory 2-
Start within 3 days to occur, it is the most clear to ovulation period.Gradually crush, dissolve after ovulation, until being changed into mottled pattern.Therefore, mesh
All products detected based on saliva of preceding in the market are in accordance with above-mentioned principle, by the observation crystallized to oneself saliva, oneself
Visually observe and the general onset of ovulation for judging tested person and the non-onset of ovulation.
But it is due to be visually observed by observing oneself, without any quantitative target, so by subjective eyesight and showing
The influence of the resolution ratio of micro mirror, and can not exclude the interference of the factors such as the optics of environment, results contrast is obscured, and only can be with
Probably extrapolate the substantially ovulation situation of measured, typically extrapolate and ovulation is would be possible within couple of days, it is impossible to determine by
Survey person is ovulated the specific time, detects that the accuracy of the onset of ovulation is relatively low, this has little significance for the women for practising contraception and becoming pregnant.
Detection method or device for more than, there is the defect of its own, therefore, and urgent need is a kind of easy to detect, accurately
Degree and higher ovulation detecting device.
The content of the invention
Ovulation device is quantitatively detected the invention provides a kind of multi-parameter based on saliva property, prior art row is solved
The problem of ovum detection time accuracy is not good.
To achieve the above object, the present invention proposes a kind of multi-parameter based on saliva property and quantitatively detects ovulation device,
Including：
Saliva collecting device, for gathering saliva, including micro fluidic device；
Electrochemical data acquisition module, electric current, current potential and impedance signal for gathering saliva electrochemistry, including collection electricity
Pole and signal processing circuit；
Data transmission module, for the data transfer that gathers electrochemical data acquisition module to data processing module；
Data processing module, for carrying out data processing and statistical analysis to the data received；And
Power module, for being powered to electrochemical data acquisition module and data transmission module.
It is preferred that, the micro fluidic device includes injection port, microchannel and chamber.
It is preferred that, one end of the acquisition electrode is located in saliva collecting device, and the other end is connected with signal processing circuit
Connect, the acquisition electrode includes a reference electrode, a working electrode and an auxiliary electrode, the reference electrode, work
The one end of electrode and auxiliary electrode in saliva collecting device is in the arc-shaped and is arranged in order from inside to outside.
It is preferred that, the reference electrode is high purity platinum gold electrode, and the working electrode and auxiliary electrode are gold electricity
Pole.
It is preferred that, the signal processing circuit includes amplifying circuit, frequency demodulation circuit, potentiostatic circuit and frequency modulation(PFM)
Circuit.
It is preferred that, the data processing module is external equipment APP modules, and operation is sent by external equipment APP modules
Instruction, is detected.
It is preferred that, the data transmission module is realized by radio connection with external equipment APP modules and is connected, described
Radio connection includes bluetooth or Wifi.
It is preferred that, also including housing, be provided with power switch on described device, shell for protecting, for starting or closing
Described device is closed, charging port and USB interface is additionally provided with.
The present invention also provides the detection method that a kind of multi-parameter based on saliva property quantitatively detects ovulation device, including such as
Lower step：
(1) saliva sample is gathered, saliva sample is collected into saliva collecting device, saliva sample is detected, is led to
The electrical circuit for crossing conducting measures temperature, electric current, current potential, impedance signal, saliva total ion concentration, luteinizing hormone concentrations, rush ovum
The plain concentration of bubble generation；
(2) the detection data that step (1) is collected are handled, the electrical parameter modulation treatment detected is included into one
Temperature, electric current, current potential, impedance signal, saliva total ion concentration, luteinizing hormone concentrations, the biology of FSH concentration
Bio signal Parameters variation process in signal parameter, analysis detection sample a cycle；
(3) the ovulation prediction date is obtained.
It is preferred that, the step (1) is carried out continuously 1-2 months, collection daily, detection 1-3 times.
Beneficial effect：
The multi-parameter based on saliva property that the present invention is provided quantitatively detects ovulation device, based on saliva in the human ovulation phase
With saliva total ion concentration in the non-onset of ovulation and FSH LH concentration be proportionate, pass through the electric current, current potential and resistance to collecting
Antinoise signal is handled and statistical analysis obtains electron stream collection of illustrative plates and impedance spectrum collection of illustrative plates, can be according to the personal data of tester essence
Really detect the ovulation period of tester；Collection in addition combined with tester's temperature data simultaneously sets up algorithm model and standard is bent
Line, further increases the ovulation period of tester.Present invention detection ovulation device is easily carried, easy to detect, efficient, and essence
Exactness is high, and device for testing ovulation date can be accurate to 2~3 hours.
Brief description of the drawings
Fig. 1 a are the structural representations of present invention detection ovulation device；
Fig. 1 b are the internal structure schematic diagrams of present invention detection ovulation device；
Fig. 2 is the schematic flow sheet of present invention detection ovulation device；
Fig. 3 is the internal electrode structure schematic diagram of present invention detection ovulation device；
Fig. 4 is the circuit diagram of present invention detection ovulation device signal processing circuit；
Fig. 5 is the curve map of the present invention detection ovulation multigroup detection of device；I. temperature curve schematic diagram；II.LH and FSH swashs
Plain concentration curve schematic diagram；III. progestational hormone and estrogen curve concentration schematic diagram；IV. saliva total ion concentration curve is illustrated
Figure；
Wherein, 1- saliva collecting devices；2-USB interfaces；3- signal processing circuits；4- power switches；5- charging ports；6-
Shell；7- reference electrodes；8- working electrodes；9- auxiliary electrodes.
Embodiment
It is below in conjunction with the accompanying drawings and specific real to enable the above objects, features and advantages of the present invention more obvious understandable
Applying mode, the present invention is further detailed explanation.
Embodiment 1
The present embodiment provides a kind of multi-parameter based on saliva property and quantitatively detects ovulation device, referring to Fig. 1 a, Fig. 1 b and
Shown in Fig. 2, including housing 6, be provided with housing saliva collecting device 1, electrochemical data acquisition module, data transmission module,
Data processing module and power module.
The saliva collecting device 1, for gathering saliva, including micro fluidic device, the micro fluidic device includes sample introduction
Mouth, microchannel and chamber, the saliva of collection pass through injection port, enter microchannel, chamber is reached afterwards.
The electrochemical data acquisition module, for gathering the electric current, current potential and impedance signal of saliva electrochemistry, including is adopted
Colelctor electrode and signal processing circuit 3；The acquisition electrode is three-electrode system, as shown in figure 3, including reference electrode, work electricity
One end of saliva contacts is in pole and auxiliary electrode, reference electrode, working electrode and auxiliary electrode and the chamber of saliva collecting device 1
Concentric circles arc, and reference electrode, working electrode and auxiliary electrode be arranged in order from inside to outside, the other end of above three electrode
It is plugged in signal processing circuit, so as to form two loops.Loop is formed between working electrode and reference electrode, for testing
The electrochemical reaction process of working electrode；Loop is formed between auxiliary electrode and reference electrode, transmission is played and electronically forms loop
Effect.The signal processing circuit 3 is loaded into the inner side of housing 6, as shown in figure 4, including amplifying circuit, frequency demodulation electricity
Road, potentiostatic circuit and frequency modulation circuit, detection obtain multi-frequency electric current and voltage difference and carry out modulation treatment, by electrical circuit
Obtained electric current, impedance information data processing are calculated as the technical parameter relevant with electrochemical ion total concentration.
Detection includes the loop of circuit turn-on twice, respectively working electrode and reference electrode formation, auxiliary electrode every time
With the loop of reference electrode formation.Circuit turn-on can all be detected to the electric current of saliva twice, the current potential of saliva and impedance letter
Number then measured respectively by the conducting of the two electrical circuits, when current potential is measured by the conducting of auxiliary electrode and reference electrode
, impedance signal is then the loop measurement of working electrode and reference electrode.Body temperature, the total ion concentration of saliva are directly to pass through
Above-mentioned electrical circuit is comprehensively obtained by the electric current, current potential, impedance information of saliva, and between lutropin, follicle-stimulating hormone (FSH)
It is directly proportional, therefore can measures indirectly to the total ion concentration in saliva.
The data transmission module is used for the data transfer that gathers electrochemical data acquisition module to data processing module.
The data processing module is used to carry out institute in data processing and statistical analysis, the present embodiment to the data received
Data processing module is stated for external equipment APP modules, is realized and is connected with data transmission module by radio connection, including indigo plant
Tooth is connected or Wifi connections, can be sent operational order by external equipment APP modules, be detected.Concrete operations include with
Lower step：Step one, wireless connection；Step 2, operation module prompting user uses step, and allows user's step in accordance with the instructions
Operation, samples and is tested；Step 3, shows testing result, provides interpretation of result；Prompting user disconnection of wireless connects afterwards
Connect and close power supply.
The power module is used to power to electrochemical data acquisition module and data transmission module, and battery conduct can be used
The power module of the present apparatus, battery case is button cell, and in the reserved charging port in end, configuration data cable completes charging work(
Energy.
Optionally, micro fluidic device described in the present embodiment can also use structure, such as Samsung disclosed in other prior arts
Electronics Co., Ltd is on November 17th, 2010, utility model patent " the miniflow device of application to Publication No. CN102071242A
Structure described in microfluidic device in part, light irradiation device, micro-fluidic system and the method for driving the system ".
It is preferred that, reference electrode described in the present embodiment is high purity platinum gold electrode, the working electrode and auxiliary electrode
It is gold electrode.
In addition, the saliva collecting device 1 of the present embodiment is arranged on detection ovulation device to be dismountable, when for same
During the detection of individual tester, the device clear water can be soaked 1-2 minutes, be reused after cleaning and drying, and can repeat
Using about 3000 times, cost is greatly reduced.
Certain tester, test a cycle detects 32 days altogether, daily average test 3 times, and the value of test is equal in whole cycle
For 1402, testing result data are 1450 during the ovulation day morning 7, for the maximum in the cycle；And 24 before and after ovulation day in
Data form a crest, thus can determine that tester's ovulation period at 19, i.e., within the cycle during test of maximum
Between add because hormone starts to be applied to postponing for 12 hours for the process spent time successfully ovulated.
In use, opening the device by the power switch set on shell, flowed into the saliva collecting device 1 of the device
Enter saliva, until three electrodes in micro fluidic device chamber are completely covered in saliva, detection is sent by external equipment APP modules
Instruction starts detection, and the testing result of the onset of ovulation can be obtained by external equipment APP modules, can also be shown and detected by the device
As a result.
During cleaning, saliva collecting device 1 and the USB interface for the junction of housing 6 for being loaded with signal processing circuit 3 are disconnected,
Remove saliva collecting device 1, with clear water soak about 1~2 minute, afterwards again by saliva collecting device 1 by USB interface with being loaded with
The housing 6 of signal processing circuit 3 is connected.
During charging, it can be connected by the charging port 5 of the ovulation detecting device afterbody with data cable, maximum charging current
No more than 8mA, constant current is charged to after 4.1V again constant pressure weak current and supplemented two to three hours.It is about 8 hours during whole charging.
Embodiment 2
The present embodiment provides the detection method that multi-parameter of the embodiment 1 based on saliva property quantitatively detects ovulation device, bag
Include following steps：
(1) saliva sample is gathered, and saliva sample is detected, including temperature, electric current, current potential, impedance signal, saliva
Total ion concentration, luteinizing hormone concentrations, FSH concentration；
(2) the detection data that step (1) is collected are handled, by the electrical parameter detected, modulation treatment is wrapped into one
Temperature, electric current, current potential, impedance signal, the bio signal parameter of saliva total ion concentration are contained, observation analysis user is at one
The change procedure of the bio signal parameter in cycle, due to the physiological signals such as total ion concentration, temperature and follicle-stimulating hormone (FSH) and Huang
Body generation element etc. can indirectly find out the variation tendency of whole ovulation process into positive correlation, using normal distribution principle,
The abnormal data caused by abnormality test is rejected, by remaining data by cycle time sequence arrangement point row, peak-peak is
Ovulation period is pointed to；As shown in figure 5, the corpus luteum generation of the body temperature curve of detection, the total ion concentration of saliva and women
Element, the change of FSH have strict corresponding relation, the electrochemistry collected by step (1) during ovulating
Data and FSH LH concentration be proportionate, and then from temperature, the electrochemical properties of saliva ion, such as electron stream, electrical impedance
The parametric inference of spectrum go out FSH LH concentration, and then judge ovulation precise time；
(3) the ovulation prediction date is obtained.
The present embodiment detection method can be accurate to 2~3 hours.Through a large amount of clinical trials, while check experiment ovulation test paper
Group (test follicle-stimulating hormone (FSH)), B ultrasound group (with ovary ultrasound diagnosis LH serum), saliva detection group of the present invention, three groups of testing result rows
Ovum day accuracy rate reaches more than 90%, wherein the detection means of the present invention detection onset of ovulation can be accurate to 2~3 hours, accurate journey
Spend apparently higher than other check devices of prior art.
Further, the step (1) is carried out continuously 1~2 month, collection daily, detection 1~3 time.
In use, by size about 8-20 mm wides, 3-5 centimetres is about, the saliva collecting device of about 3--15 millimeters of thickness puts
It is placed in tester oral cavity, places about 5-30 seconds, using capillary theory, the saliva of collection passes through injection port, enters
Microchannel, reaches chamber, gathers the saliva of about several hectolambdas, takes out afterwards.Saliva sample is collected in micro fluidic device
In chamber, instrument power source is opened.Connect bluetooth.And start two loop starts on test, electrode, and return to electric ginseng
Number, after modulation treatment, obtains the relevant parameter of saliva total ion concentration.It is last to show final parameter in APP.
The detection of the onset of ovulation is carried out using the present apparatus, using needing the early stage learning process of 1~2 month, that is, is used
Person needs continuously to measure the bimestrial time, detects 1~3 time daily, record testing result, and by software systems to collection at that time
Data carry out statistical analysis, set up personalized model and algorithm, it is comprehensive for each according to data, model and algorithm is obtained
The specific data of tester, with depth learning technology, set up corresponding basic standard curve, the ovulation situation to tester is entered
Row precisely detection and judgement.
The embodiment of the present invention is described in detail above, specific case used herein to the principle of the present invention and
Embodiment is set forth, and the explanation of above example is only intended to help and understands the method for the present invention and its core concept；
Simultaneously for those of ordinary skill in the art, according to the thought of the present invention, can in specific embodiments and applications
There is change part, in summary, this specification content should not be construed as limiting the invention.
Claims (10)
1. a kind of multi-parameter based on saliva property quantitatively detects ovulation device, it is characterised in that including：
Saliva collecting device, for gathering saliva, including micro fluidic device；
Electrochemical data acquisition module, electric current, current potential and impedance signal for gathering saliva electrochemistry, including acquisition electrode and
Signal processing circuit；
Data transmission module, for the data transfer that gathers electrochemical data acquisition module to data processing module；
Data processing module, for carrying out data processing and statistical analysis to the data received；And
Power module, for being powered to electrochemical data acquisition module and data transmission module.
2. the multi-parameter according to claim 1 based on saliva property quantitatively detects ovulation device, it is characterised in that described
Micro fluidic device includes injection port, microchannel and chamber.
3. the multi-parameter according to claim 1 based on saliva property quantitatively detects ovulation device, it is characterised in that described
One end of acquisition electrode is located in saliva collecting device, and the other end is connected with signal processing circuit, and the acquisition electrode includes
One reference electrode, a working electrode and an auxiliary electrode, the reference electrode, working electrode and auxiliary electrode are located at saliva
One end in liquid harvester is in the arc-shaped and is arranged in order from inside to outside.
4. the multi-parameter according to claim 3 based on saliva property quantitatively detects ovulation device, it is characterised in that described
Reference electrode is high purity platinum gold electrode, and the working electrode and auxiliary electrode are gold electrode.
5. the multi-parameter according to claim 1 based on saliva property quantitatively detects ovulation device, it is characterised in that described
Signal processing circuit includes amplifying circuit, frequency demodulation circuit, potentiostatic circuit and frequency modulation circuit.
6. the multi-parameter according to claim 1 based on saliva property quantitatively detects ovulation device, it is characterised in that described
Data processing module is external equipment APP modules, sends operational order by external equipment APP modules, is detected.
7. the multi-parameter according to claim 6 based on saliva property quantitatively detects ovulation device, it is characterised in that described
Data transmission module realizes and is connected that the radio connection includes indigo plant with external equipment APP modules by radio connection
Tooth or Wifi.
8. the multi-parameter according to claim 1 based on saliva property quantitatively detects ovulation device, it is characterised in that also wrap
Housing is included, be provided with power switch on described device, shell for protecting, for starting or closing described device, be additionally provided with
Charging port and USB interface.
9. any multi-parameters based on saliva property of claim 1-8 quantitatively detect the detection method of ovulation device, it is special
Levy and be, comprise the following steps：
(1) saliva sample is gathered, saliva sample is collected into saliva collecting device, saliva sample is detected, by leading
Logical electrical circuit measures temperature, electric current, current potential, impedance signal, saliva total ion concentration, luteinizing hormone concentrations, rush ovarian follicle life
Into plain concentration；
(2) the detection data that step (1) is collected are handled, the electrical parameter modulation treatment detected is included into temperature into one
Degree, electric current, current potential, impedance signal, saliva total ion concentration, luteinizing hormone concentrations, the biological letter of FSH concentration
Bio signal Parameters variation process in number parameter, analysis detection sample a cycle；
(3) the ovulation prediction date is obtained.
10. detection method according to claim 9, it is characterised in that the step (1) is carried out continuously 1-2 individual month, daily
Collection, detection 1-3 times.
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