CN102901759A - Thin layer detection pool for detecting serotonins and metabolites in urine sample, and its manufacturing method - Google Patents
Thin layer detection pool for detecting serotonins and metabolites in urine sample, and its manufacturing method Download PDFInfo
- Publication number
- CN102901759A CN102901759A CN 201110210411 CN201110210411A CN102901759A CN 102901759 A CN102901759 A CN 102901759A CN 201110210411 CN201110210411 CN 201110210411 CN 201110210411 A CN201110210411 A CN 201110210411A CN 102901759 A CN102901759 A CN 102901759A
- Authority
- CN
- China
- Prior art keywords
- electrode
- bromophenol blue
- urine sample
- thin layer
- poly
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Abstract
A thin layer detection pool for detecting serotonins and metabolites in a urine sample comprises a work electrode, a reference electrode and an auxiliary electrode, wherein the input ends of the work electrode, the reference electrode and the auxiliary electrode are connected with the output end of a liquid chromatograph; the output ends of the work electrode, the reference electrode and the auxiliary electrode are connected with the input end of an electrochemical system; the output end of the electrochemical system is connected with a computer signal acquisition end; the work electrode comprises a precious metal particle layer, a poly(bromophenol blue) layer and an electrode matrix; precious metal particles are deposited on a poly(bromophenol blue) membrane; and poly(bromophenol blue) is deposited on the electrode matrix. The thin layer detection pool manufactured in the invention can realize the detection of concentrations of the serotonins and metabolites thereof in the urine sample through simple steps, the detection linearity range can reach 1.0*10<-9>-1.0*10<-5>mol/L, and the detection resolution is 1.0*10<-10>mol/L. The detection of the concentrations of the serotonins and the metabolites thereof in the urine sample can be realized through the simple steps.
Description
Technical field
What the present invention relates to is the device in a kind of electrochemical measuring technique field and preparation method thereof, specifically a kind of thin layer detection cell and preparation method for urine sample thrombocytin and metabolic product detection.
Background technology
Thrombocytin is serotonin, is present in the argentaffin of mast cell, blood platelet and intestinal mucosa.Studies show that appendix is inflamed causes mast cell, basocyte to take off particle, discharges the inflammatory mediators such as histamine, serotonin, and the concentration of thrombocytin increases in the blood.Thrombocytin under the effect of monoamine oxidase in vivo rapidly metabolism generate 5-hydroxyindoleacetic acid, from urine, get rid of.Therefore the mensuration of serotonin and metabolic product 5-hydroxyindoleacetic acid content thereof has some reference value to typhlitic diagnosis in the urine sample.
Multiple detection technique has been arranged for the mensuration of serotonin and 5-hydroxyindoleacetic acid at present, such as photometry, fluorescence method, chemoluminescence method, polarography, voltammetry and Capillary Electrophoresis etc.Although said method has been used for the detection of biological and drug sample serotonin and 5-hydroxyindoleacetic acid, much methods exist the pre-service complexity, cost is high, sensitivity is low and can not measure simultaneously the shortcoming of these compounds on same biological sample and affected the application of these methods.
Summary of the invention
The present invention is directed to the prior art above shortcomings, a kind of thin layer detection cell that detects for urine sample thrombocytin and metabolic product thereof and preparation method thereof is provided, realize detection to thrombocytin in the urine sample and Metabolites Concentration thereof by easy steps.
The invention provides the thin layer detection cell that thrombocytin and metabolic product detect in a kind of urine sample, comprise: working electrode, contrast electrode and auxiliary electrode, wherein: working electrode, contrast electrode, the input end of auxiliary electrode is connected with the output terminal of liquid chromatography, working electrode, contrast electrode, the output terminal of auxiliary electrode is connected with the input end of electro-chemical systems, the output terminal of electro-chemical systems is connected with the Computer signal collection terminal, it is characterized in that, described working electrode comprises: the noble metal layer, poly-bromophenol blue layer and electrode matrix, wherein: noble metal is deposited on the poly-bromophenol blue film, and poly-bromophenol blue is deposited on the electrode matrix.The thickness of noble metal layer is 40～200 nanometers in the described working electrode, and the thickness of poly-bromophenol blue film is 30～500 nanometers.
Described electrode matrix is the electrode matrix of glass carbon material.
Described liquid chromatography adopts Luna 5 μ m C
18Reverse-phase chromatographic column.
The invention provides the preparation method of the thin layer detection cell that thrombocytin and metabolic product detect in a kind of urine sample, it is characterized in that, may further comprise the steps:
The first step: the preparation of poly-bromophenol blue modified electrode: at first glass-carbon electrode is carried out ultrasonic cleaning in redistilled water after the polishing; Then the glass-carbon electrode after cleaning is put into phosphate solution and is carried out the scan round processing; Again the glass-carbon electrode after the scan round is put into the phosphate solution that solute is bromophenol blue, again carried out scan round and process; Clean with redistilled water after will taking out through the glass-carbon electrode after the secondary cycle scan process, make poly-bromophenol blue modified electrode;
Second step: noble metal loads: at first will gather the bromophenol blue modified electrode and place and contain solvable row precious metal salt solution, carry out taking out after scan round is processed the poly-bromophenol blue modified electrode through overscanning, wash in turn successively several times with absolute ethyl alcohol and redistilled water, the poly-bromophenol blue modified electrode after obtaining noble metal and loading is as working electrode;
The 3rd step: successively contrast electrode, working electrode and auxiliary electrode are linked to each other with liquid chromatography and electro-chemical systems, wherein: contrast electrode is the Ag/AgCl electrode; Auxiliary electrode is the electrode that stainless steel is made, and makes the thin layer detection cell for urine sample thrombocytin and metabolic product detection thereof.
Refer to working electrode, contrast electrode and auxiliary electrode are linked to each other with electro-chemical systems respectively in the processing of the scan round described in the first step, in the voltage range of setting, carry out cycle potentials scanning with certain sweep speed.
The concentration of bromophenol blue is 1.0 * 10 in the phosphate solution described in the first step
-5Mol/L～0.5mol/L.
Phosphate buffered solution described in the first step refers to: phosphate concentration is potassium dihydrogen phosphate and the disodium phosphate soln of 0.2mol/L.
The mobile phase that liquid chromatography described in the 3rd step is used is the phosphate buffered solution of the pH5.0 that contains 5%～50% methyl alcohol and 0.2mol/L～7.0, and the flow velocity of this mobile phase is 1.0mL/min.
The present invention detects by following steps:
At first open liquid chromatography and electro-chemical systems so that the serotonin of different concentration known and 5-hydroxyindoleacetic acid sample with phosphate buffered solution through Luna 5 μ m C
18Reverse-phase chromatographic column flows through the surface of contrast electrode, working electrode and auxiliary electrode successively;
Then utilize CHI832 type electro-chemical systems to record the current signal of different Sample producings, carry out match according to the current value of the response current of the known serotonin under the different samples and 5-hydroxyindoleacetic acid concentration and corresponding actual measurement as coordinate in length and breadth, the thin layer detection cell is carried out sample learning;
The urine sample 20 μ L that will process again place and prepare test environment, record the current value of response current, read serotonin contained in urine sample corresponding to this current value and 5-hydroxyindoleacetic acid concentration according to the serotonin that obtains through sample learning and the linear relationship chart of 5-hydroxyindoleacetic acid concentration-corresponding electric current.
The method of the invention can realize with easy steps the detection of serotonin and 5-hydroxyindoleacetic acid concentration, detects the range of linearity and can be reached for 1.0 * 10
-9～1.0 * 10
-5Mol/L, detection resolution is 1.0 * 10
-10Mol/L.
Description of drawings
Fig. 1 is that the present invention uses fundamental diagram.
Fig. 2 is the working electrode schematic diagram.
Specific embodiment
Embodiment implements under take technical solution of the present invention as prerequisite, provided detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.
The thin layer detection cell that thrombocytin and metabolic product detect in a kind of urine sample, comprise: working electrode, contrast electrode and auxiliary electrode, wherein: the input end of working electrode, contrast electrode, auxiliary electrode is connected with the output terminal of liquid chromatography, the output terminal of working electrode, contrast electrode, auxiliary electrode is connected with the input end of electro-chemical systems, and the output terminal of electro-chemical systems is connected with the Computer signal collection terminal; Described working electrode comprises: noble metal layer, poly-bromophenol blue layer and electrode matrix, wherein: noble metal is deposited on the poly-bromophenol blue film, and poly-bromophenol blue is deposited on the electrode matrix.
The glass-carbon electrode that pre-service is good is put into the phosphate buffer solution of pH=7.0, with the sweep speed of 20mV/s-0.2V～+ carry out scan round in the potential range of 2.0V to process 10min.
Then electrode is put into the phosphate buffered solution of the pH=7.0 that contains the 0.5mol/L bromophenol blue, with the sweep speed of 20mV/s 0.0V～+ carry out scan round in the potential range of 1.0V to process 6 circles, obtain poly-bromophenol blue modified electrode;
To gather the bromophenol blue modified electrode places and contains 1.0 * 10
-3The K of mol/L
2PtCl
6In the KCl solution of 0.1mol/L, with the sweep speed of 0.1V/s-0.4V～+ carry out scan round in the potential range of 0.3V to process 12 circles, obtain the poly-bromophenol blue modified electrode after platinum particles loads, wherein: poly-bromophenol blue layer and platinum grain sublayer are coated on the glass-carbon electrode outside successively from inside to outside, the thickness of platinum grain sublayer is 50～70nm, and the thickness of poly-bromophenol blue layer is 70～90nm;
Poly-bromophenol blue modified electrode after loading take platinum particles is as working electrode, the Ag//AgCl electrode is as contrast electrode, stainless steel is auxiliary electrode, successively contrast electrode, working electrode and auxiliary electrode are linked to each other with liquid chromatography and electro-chemical systems, separate by Luna5 μ m C18 reverse-phase chromatographic column, mobile phase wherein is to contain in the phosphate buffered solution of pH6.0 of 10% methyl alcohol and 0.2mol/L, and flow velocity is 1.0mL/min;
Then under loading+0.6V current potential, according to the known serotonin under the different samples and 5-hydroxyindoleacetic acid concentration and utilize the current value of the response current of the corresponding actual measurement of CHI832 type electro-chemical systems to carry out match as coordinate in length and breadth, the thin layer detection cell is carried out sample learning;
Embodiment 2
Thrombocytin and metabolic product detect in a kind of urine sample thin layer detection cell and preparation method, the glass-carbon electrode that pre-service is good is put into the phosphate buffered solution of pH=5.0, with the sweep speed of 15mV/s-0.2V～+ carry out scan round in the potential range of 1.0V to process 15min;
Then electrode is put into and contained 1.0 * 10
-3In the phosphate buffered solution of the pH=5.0 of mol/L bromophenol blue, with the sweep speed of 15mV/s 0.0V～+ carry out scan round in the potential range of 1.0V to process 10 circles, obtain poly-bromophenol blue modified electrode;
To gather the bromophenol blue modified electrode places and contains 2.5 * 10
-3The K of mol/L
2PtCl
6In the KCl solution of 0.1mol/L, with the sweep speed of 0.1V/s-0.4V～+ carry out scan round in the potential range of 0.3V to process 16 circles, obtain the poly-bromophenol blue modified electrode after platinum particles loads, wherein: poly-bromophenol blue layer and platinum grain sublayer are coated on the glass-carbon electrode outside successively from inside to outside, the thickness of platinum grain sublayer is 80～100nm, and the thickness of poly-bromophenol blue layer is 50～70nm;
Poly-bromophenol blue modified electrode after loading take platinum particles is as working electrode, and the Ag-AgCl electrode is as contrast electrode, and stainless steel is auxiliary electrode, successively contrast electrode, working electrode and auxiliary electrode is linked to each other with liquid chromatography and electro-chemical systems, by Luna5 μ m C
18Reverse-phase chromatographic column separates, and mobile phase wherein is to contain in the phosphate buffered solution of pH5.0 of 10% methyl alcohol and 0.2mol/L, and flow velocity is 1.0mL/min;
Then under loading+0.6V current potential, carry out match according to the current value of the known serotonin under the different samples and 5-hydroxyindoleacetic acid concentration and the corresponding response current that utilizes the actual measurement of CHI832 type electro-chemical systems as coordinate in length and breadth, the thin layer detection cell is carried out sample learning;
Thrombocytin and metabolic product detect in a kind of urine sample thin layer detection cell and preparation method, the glass-carbon electrode that pre-service is good is put into the phosphate buffered solution of pH=7.0, with the sweep speed of 20mV/s-0.2V～+ carry out scan round in the potential range of 2.0V to process 50min;
Then electrode is put into and contained 1.0 * 10
-5In the phosphate buffered solution of the pH=7.0 of mol/L bromophenol blue, with the sweep speed of 20mV/s 0.0V～+ carry out scan round in the potential range of 1.0V to process 30 circles, obtain poly-bromophenol blue modified electrode;
To gather the bromophenol blue modified electrode places and contains 4.5 * 10
-3The K of mol/L
2PtCl
6In the KCl solution of 0.1mol/L, with the sweep speed of 0.1V/s-0.4V～+ carry out scan round in the potential range of 0.3V to process 18 circles, obtain the poly-bromophenol blue modified electrode after platinum particles loads, wherein: poly-bromophenol blue layer and platinum grain sublayer are coated on the glass-carbon electrode outside successively from inside to outside, the thickness of platinum grain sublayer is 100～120nm, and the thickness of poly-bromophenol blue layer is 110～130nm;
Poly-bromophenol blue modified electrode after loading take platinum particles is as working electrode, and the Ag-AgCl electrode is as contrast electrode, and stainless steel is auxiliary electrode, successively contrast electrode, working electrode and auxiliary electrode is linked to each other with liquid chromatography and electro-chemical systems, by Luna5 μ m C
18Reverse-phase chromatographic column separates, and mobile phase wherein is to contain in the phosphate buffered solution of pH7.0 of 30% methyl alcohol and 0.2mol/L, and flow velocity is 1.0mL/min;
Then under loading+0.6V current potential, carry out match according to the current value of the known serotonin under the different samples and 5-hydroxyindoleacetic acid concentration concentration and the corresponding response current that utilizes the actual measurement of CHI832 type electro-chemical systems as coordinate in length and breadth, the thin layer detection cell is carried out sample learning;
Claims (9)
1. the thin layer detection cell that thrombocytin and metabolic product detect in the urine sample, comprise: working electrode, contrast electrode and auxiliary electrode, wherein: working electrode, contrast electrode, the input end of auxiliary electrode is connected with the output terminal of liquid chromatography, working electrode, contrast electrode, the output terminal of auxiliary electrode is connected with the input end of electro-chemical systems, the output terminal of electro-chemical systems is connected with the Computer signal collection terminal, it is characterized in that, described working electrode comprises: the noble metal layer, poly-bromophenol blue layer and electrode matrix, wherein: noble metal is deposited on the poly-bromophenol blue film, and poly-bromophenol blue is deposited on the electrode matrix.
2. the thin layer detection cell that thrombocytin and metabolic product detect in the urine sample according to claim 1 is characterized in that the thickness of noble metal layer is 40～200 nanometers in the described working electrode, and the thickness of poly-bromophenol blue film is 30～500 nanometers.
3. the thin layer detection cell that thrombocytin and metabolic product detect in the urine sample according to claim 1 and 2 is characterized in that described electrode matrix is the electrode matrix of glass carbon material.
4. the thin layer detection cell that thrombocytin and metabolic product detect in the urine sample according to claim 3 is characterized in that, described liquid chromatography adopts Luna 5 μ m C
18Reverse-phase chromatographic column.
5. the preparation method of the thin layer detection cell that thrombocytin and metabolic product detect in the urine sample according to claim 4 is characterized in that, may further comprise the steps:
The first step: the preparation of poly-bromophenol blue modified electrode: at first glass-carbon electrode is carried out ultrasonic cleaning in redistilled water after the polishing; Then the glass-carbon electrode after cleaning is put into phosphate solution and is carried out the scan round processing; Again the glass-carbon electrode after the scan round is put into the phosphate solution that solute is bromophenol blue, again carried out scan round and process; Clean with redistilled water after will taking out through the glass-carbon electrode after the secondary cycle scan process, make poly-bromophenol blue modified electrode;
Second step: noble metal loads: at first will gather the bromophenol blue modified electrode and place and contain solvable row precious metal salt solution, carry out taking out after scan round is processed the poly-bromophenol blue modified electrode through overscanning, wash in turn successively several times with absolute ethyl alcohol and redistilled water, the poly-bromophenol blue modified electrode after obtaining noble metal and loading is as working electrode;
The 3rd step: successively contrast electrode, working electrode and auxiliary electrode are linked to each other with liquid chromatography and electro-chemical systems, wherein: contrast electrode is the Ag/AgCl electrode; Auxiliary electrode is the electrode that stainless steel is made, and makes the thin layer detection cell for urine sample thrombocytin and metabolic product detection thereof.
6. the preparation method of the thin layer detection cell that thrombocytin and metabolic product detect in the urine sample according to claim 5, it is characterized in that, refer to working electrode, contrast electrode and auxiliary electrode are linked to each other with electro-chemical systems respectively in the processing of the scan round described in the first step, in the voltage range of setting, carry out cycle potentials scanning with certain sweep speed.
7. the preparation method of the thin layer detection cell that thrombocytin and metabolic product detect in the urine sample according to claim 5 is characterized in that the concentration of bromophenol blue is 1.0 * 10 in the phosphate solution described in the first step
-5Mol/L～0.5mol/L.
8. the preparation method of the thin layer detection cell that thrombocytin and metabolic product detect in the urine sample according to claim 5, it is characterized in that, the phosphate buffered solution described in the first step refers to: phosphate concentration is potassium dihydrogen phosphate and the disodium phosphate soln of 0.2mol/L.
9. the preparation method of the thin layer detection cell that thrombocytin and metabolic product detect in the urine sample according to claim 5, it is characterized in that, the mobile phase that liquid chromatography described in the 3rd step is used is the phosphate buffered solution of the pH5.0 that contains 5%～50% methyl alcohol and 0.2mol/L～7.0, and the flow velocity of this mobile phase is 1.0mL/min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110210411 CN102901759A (en) | 2011-07-26 | 2011-07-26 | Thin layer detection pool for detecting serotonins and metabolites in urine sample, and its manufacturing method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110210411 CN102901759A (en) | 2011-07-26 | 2011-07-26 | Thin layer detection pool for detecting serotonins and metabolites in urine sample, and its manufacturing method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102901759A true CN102901759A (en) | 2013-01-30 |
Family
ID=47574112
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110210411 Pending CN102901759A (en) | 2011-07-26 | 2011-07-26 | Thin layer detection pool for detecting serotonins and metabolites in urine sample, and its manufacturing method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102901759A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105223249A (en) * | 2015-10-16 | 2016-01-06 | 上海纳米技术及应用国家工程研究中心有限公司 | A kind of preparation method of the chemical sensor for thrombocytin detection |
| CN106959326A (en) * | 2017-05-19 | 2017-07-18 | 量准（上海）实业有限公司 | Multi-parameter based on saliva property quantitatively detects ovulation device |
| CN109187842A (en) * | 2018-11-13 | 2019-01-11 | 吕梁学院 | Serotonin extracts the method examined with assay in a kind of sea-buckthorn |
| CN110441371A (en) * | 2019-08-22 | 2019-11-12 | 重庆医科大学 | A kind of highly sensitive indoles electrochemical detection method |
-
2011
- 2011-07-26 CN CN 201110210411 patent/CN102901759A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105223249A (en) * | 2015-10-16 | 2016-01-06 | 上海纳米技术及应用国家工程研究中心有限公司 | A kind of preparation method of the chemical sensor for thrombocytin detection |
| CN105223249B (en) * | 2015-10-16 | 2018-01-16 | 上海纳米技术及应用国家工程研究中心有限公司 | A kind of preparation method of chemical sensor for thrombocytin detection |
| CN106959326A (en) * | 2017-05-19 | 2017-07-18 | 量准（上海）实业有限公司 | Multi-parameter based on saliva property quantitatively detects ovulation device |
| CN109187842A (en) * | 2018-11-13 | 2019-01-11 | 吕梁学院 | Serotonin extracts the method examined with assay in a kind of sea-buckthorn |
| CN109187842B (en) * | 2018-11-13 | 2021-10-08 | 吕梁学院 | Method for extracting, detecting and measuring content of 5-hydroxytryptamine in sea buckthorn |
| CN110441371A (en) * | 2019-08-22 | 2019-11-12 | 重庆医科大学 | A kind of highly sensitive indoles electrochemical detection method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Babaei et al. | A glassy carbon electrode modified with multiwalled carbon nanotube/chitosan composite as a new sensor for simultaneous determination of acetaminophen and mefenamic acid in pharmaceutical preparations and biological samples | |
| Norouzi et al. | Fast monitoring of nano‐molar level of gentamycin by fast Fourier transform continuous cyclic voltammetry in flowing solution | |
| CN106501332B (en) | Zn-MOFs/ glass-carbon electrode and preparation method thereof and application | |
| Antal et al. | Statins determination: A review of electrochemical techniques | |
| Tabanlıgil Calam et al. | Electrochemical determination of 8-hydroxyquinoline in a cosmetic product on a glassy carbon electrode modified with 1-amino-2-naphthol-4-sulphonic acid | |
| Liu et al. | Simultaneous and sensitive voltammetric determination of acetaminophen and its degradation product for pharmaceutical quality control and pharmacokinetic research by using ultrathin poly (calconcarboxylic acid) film modified glassy carbon electrode | |
| Mielech-Łukasiewicz et al. | Electrooxidation of some antifungal agents and their square-wave voltammetric determination in cosmetics and pharmaceutics | |
| CN102901759A (en) | Thin layer detection pool for detecting serotonins and metabolites in urine sample, and its manufacturing method | |
| CN104597090A (en) | Enzyme-free potentiometric glucose sensor and detection method thereof | |
| Santos et al. | Evaluation of the potentialities of a carbon nanotubes/silicone rubber composite electrode in the determination of hydrochlorothiazide | |
| Chebotarev et al. | Determination of levodopa in pharmaceuticals using a disposable electrochemically activated carbon-paste electrode by linear sweep voltammetry | |
| Filik et al. | Poly (2, 2′-(1, 4-phenylenedivinylene) Bis-8-hydroxyquinaldine) modified glassy carbon electrode for the simultaneous determination of paracetamol and p-Aminophenol | |
| Norouzi et al. | Ultrasensitive flow-injection electrochemical method using fast fourier transform square-wave voltammetry for detection of vitamin B1 | |
| Demircan et al. | Electroanalytical characterization of verapamil and its voltammetric determination in pharmaceuticals and human serum | |
| CN104614426B (en) | The glass-carbon electrode of poly- bromocresol green modification and its application | |
| Sattayasamitsathit et al. | Bismuth film electrode for analysis of tetracycline in flow injection system | |
| CN106872544A (en) | A kind of preparation method of poly- methyl blue/acetylene black composite modified glassy carbon electrode and its application in paracetamol is determined | |
| Dilgin | Determination of calcium dobesilate by differential pulse voltammetry at a disposable pencil graphite electrode | |
| Teradale et al. | Electrochemical investigation of allopurinol polymerised carbon paste electrode interface for epinephrine and folic acid sensing in pharmaceutical samples | |
| CN105223249A (en) | A kind of preparation method of the chemical sensor for thrombocytin detection | |
| CN104020205B (en) | The imprinted polymer membrane electrode of parachloroanilinum, its preparation method and application process | |
| CN105606670A (en) | Electrochemical method for detecting polycyclic aromatic hydrocarbons in high-sensitivity manner | |
| CN110031526A (en) | One kind being based on K2Fe4O7The dopamine of electrode is without enzyme sensor, preparation method and applications | |
| CN1865967A (en) | Process for preparing O-carboxymethyl chitosan covalent binding chemical modification electrode | |
| Bozal et al. | Quantitative analysis of irbesartan in pharmaceuticals and human biological fluids by voltammetry |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130130 |