CN1945289A - Micro electrochemical luminescence detecting pool - Google Patents
Micro electrochemical luminescence detecting pool Download PDFInfo
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- CN1945289A CN1945289A CN 200610069123 CN200610069123A CN1945289A CN 1945289 A CN1945289 A CN 1945289A CN 200610069123 CN200610069123 CN 200610069123 CN 200610069123 A CN200610069123 A CN 200610069123A CN 1945289 A CN1945289 A CN 1945289A
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- electrochemical luminescence
- storage tank
- luminescence detecting
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Abstract
The micro electrochemical luminescence detecting cell includes one transparent plate, one transparent panel covering the transparent plate, one micro liquid storage cell formed through drilling one hole in the transparent panel, one reference electrode and one auxiliary electrode set inside the micro liquid storage cell, one work electrode set detachably on the support over the micro liquid storage cell. The micro electrochemical luminescence detecting cell has simple structure, easy regulation and use, low measured liquid consumption and low test cost.
Description
Technical field
The present invention relates to the electrochemical analysis field, relate to a kind of design and making of micro electrochemical luminescence detecting pool more specifically.
Background technology
Electrochemical luminescence detecting pool is the device that is used for detecting and study the electrogenerated chemiluminescence activity of material.At present, electrochemical luminescence detecting pool commonly used is to carry out simple refit to obtain on the basis of three-electrode electro Chemical detection cell, promptly places a photomultiplier in the bottom of glass (or quartzy) beaker, is used to measure the luminous signal from working electrode surface.Because existing electrochemical luminescence detecting pool and electrochemical investigating pond do not have the difference of essence, use and mainly contain following defective: the volume of (1) detection cell is excessive, and the use amount of medicine or sample is too many; (2) position of working electrode is unfixing, influences the laboratory reappearance.Volume is excessive to be the most outstanding deficiency of present electrochemical luminescence detecting pool, and the volume of solution requires usually about 5 to 10 milliliters, and electrochemiluminescence uses expensive luminescence reagent usually, and as bipyridyl ruthenium, so experimental cost is higher.The working electrode position of existing electrochemical luminescence detecting pool is unfixing in addition, influences the reappearance that light intensity is measured.If can use volume as the micro electrochemical luminescence detecting pool of micro updating, will obviously reduce the use amount of test fluid, reduce experimental cost, enlarge range of application.
Summary of the invention
The objective of the invention is to develop a kind of micro electrochemical luminescence detecting pool in order to overcome that existing electrochemical luminescence detecting pool exists that volume is big, working electrode takes out inconvenience and the position such as is not easy to fix at shortcoming.
Micro electrochemical luminescence detecting pool of the present invention is established and is comprised light-passing board, be coated with light penetrating panel on the light-passing board, described light penetrating panel is carved with a cave to form micro-liquid storage tank on light-passing board, be respectively equipped with contrast electrode and auxiliary electrode in the described micro-liquid storage tank, the locating support of trace liquid storage tank top is provided with the working electrode that points to micro-liquid storage tank, and described working electrode and locating support are releasable connection.
The present invention one is because with the clear sheet formation, so simple structure is easy to make; The 2nd, so because working electrode and locating support are releasable connection helps user's demolition, installation and clean use.The volume that the present invention has overcome existing electrochemical investigating pond is big, working electrode takes out inconvenience and the position such as is not easy to fix at shortcoming, volume with the required test liquid of micro electrochemical luminescence detecting pool is little, and remarkable advantages such as convenient are transferred, handled in the working electrode position.
Adopt micro electrochemical luminescence detecting pool of the present invention, when pond internal diameter 10mm, test fluid height 1mm, can realize that electrochemiluminescence detects required liquor capacity below 100 μ L, thereby, significantly reduce the use amount of medicine than hanging down 2 orders of magnitude with conventional electrochemical luminescence detecting pool.
Description of drawings
Fig. 1 is the vertical view of micro electrochemical luminescence detecting pool of the present invention, wherein: A. bottom organic glass sheet; B. top organic glass sheet; The C.Ag/AgCl contrast electrode; D. platinum loop auxiliary electrode; E. the projection of working electrode on light inlet window; F. porous glass material: G. fixed screw.
Fig. 2 is the side view of the present invention's one micro electrochemical luminescence detecting pool, wherein: A. bottom organic glass sheet; B. top organic glass sheet; The C.Ag/AgCl contrast electrode; D. platinum loop auxiliary electrode; E. working electrode; F. porous glass material; G. fixed screw; H. adjusting screw; I. plexiglass block; PMT: photomultiplier.
Embodiment
Micro electrochemical luminescence detecting pool of the present invention comprises light-passing board, be coated with light penetrating panel on the light-passing board, described light penetrating panel is carved with a cave to form micro-liquid storage tank on light-passing board, be respectively equipped with contrast electrode and auxiliary electrode in the described micro-liquid storage tank, the locating support of trace liquid storage tank top is provided with the working electrode that points to micro-liquid storage tank, and described working electrode and locating support are releasable connection.
In order to help regulating working depth and demolition, installation, use, locating support is connected with working electrode through lifting regulating mechanism.Described lifting regulating mechanism is the perpendicular adjusting screw of being located on the locating support threaded hole, and working electrode passes the duct, middle part of adjusting screw.
Above-mentioned contrast electrode passes light-passing board, stretches into the duct identical with micro-liquid storage tank.Described auxiliary electrode passes light penetrating panel, and its inserting end that stretches into micro-liquid storage tank bottom circularizes and be set around micro-liquid storage tank bottom.
Among the embodiment, described auxiliary electrode is a platinum filament, and is fixed on micro-liquid storage tank bottom by epoxy resin.Described light penetrating panel and locating support material are the organic glass sheet.
Embodiment
Get a slice well cutting the thick organic glass sheet of 1cm (see Fig. 1 or Fig. 2, A), in its left side toward drilled in middle one circular hole, in fill 3mol/L KCl solution, rubber plug beyond the Great Wall fills KCl solution and makes the Ag/AgCl contrast electrode and (see Fig. 1 or Fig. 2, C) in then the Ag/AgCl silk being inserted; (see Fig. 1 or Fig. 2, B), form volume at the middle part of two poly (methyl methacrylate) plates (A and B) like this is the detection cell of micro updating to the organic glass sheet of thick with the bonding a slice 0.25cm of epoxy resin above the middle part of organic glass sheet A, hollow (1cm diameter); Fixing an annular platinum filament in bottom, the edge in pond with epoxy resin makes auxiliary electrode and (sees Fig. 1 or Fig. 2, D); Left bottom at detection cell is bored an aperture that communicates with contrast electrode, imbed how empty glass material at this aperture and (see Fig. 1 or Fig. 2, F), making the KCl solution of contrast electrode carry out slow mass exchange by fritted glass and detection cell solution is electrically connected guaranteeing; Working electrode is placed on the top, middle part of trace amount detection pool and (sees Fig. 1 or Fig. 2, E), adjusting screw (Fig. 2 of working electrode elder generation's overcoat, H), purpose is convenient to the taking-up processing of electrode and the accurate control of electrode position, (Fig. 2, I) (Fig. 1 or Fig. 2 G) are fixed on the detection cell with four pieces of fixed screws by a plexiglass block then.
Claims (7)
1. micro electrochemical luminescence detecting pool, it is characterized in that: comprise light-passing board, be coated with light penetrating panel on the light-passing board, described light penetrating panel is carved with a cave to form micro-liquid storage tank on light-passing board, be respectively equipped with contrast electrode and auxiliary electrode in the described micro-liquid storage tank, the locating support of trace liquid storage tank top is provided with the working electrode that points to micro-liquid storage tank, and described working electrode and locating support are releasable connection.
2. micro electrochemical luminescence detecting pool according to claim 1 is characterized in that: locating support is connected with working electrode through lifting regulating mechanism.
3. micro electrochemical luminescence detecting pool according to claim 1 and 2 is characterized in that: described lifting regulating mechanism is the perpendicular adjusting screw of being located on the locating support threaded hole, and working electrode passes the duct, middle part of adjusting screw.
4. micro electrochemical luminescence detecting pool according to claim 3 is characterized in that: described contrast electrode passes light-passing board, stretches into the duct identical with micro-liquid storage tank.
5. micro electrochemical luminescence detecting pool according to claim 4 is characterized in that: described auxiliary electrode passes light penetrating panel, and its inserting end that stretches into micro-liquid storage tank bottom circularizes and be set around micro-liquid storage tank bottom.
6. micro electrochemical luminescence detecting pool according to claim 5 is characterized in that: described auxiliary electrode is a platinum filament, and is fixed on micro-liquid storage tank bottom by epoxy resin.
7. micro electrochemical luminescence detecting pool according to claim 6 is characterized in that: described light penetrating panel and locating support material are the organic glass sheet.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100691236A CN100451626C (en) | 2006-09-30 | 2006-09-30 | Micro electrochemical luminescence detecting pool |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100691236A CN100451626C (en) | 2006-09-30 | 2006-09-30 | Micro electrochemical luminescence detecting pool |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1945289A true CN1945289A (en) | 2007-04-11 |
| CN100451626C CN100451626C (en) | 2009-01-14 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2006100691236A Expired - Fee Related CN100451626C (en) | 2006-09-30 | 2006-09-30 | Micro electrochemical luminescence detecting pool |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106959326A (en) * | 2017-05-19 | 2017-07-18 | 量准（上海）实业有限公司 | Multi-parameter based on saliva property quantitatively detects ovulation device |
| CN107589157A (en) * | 2017-11-01 | 2018-01-16 | 青岛科技大学 | Photic electrochemical sensor |
| CN110579519A (en) * | 2019-09-19 | 2019-12-17 | 常熟理工学院 | electrochemical-controlled rapid COD detection device and method thereof |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103149192B (en) * | 2013-02-22 | 2015-05-13 | 厦门大学 | In-situ electrochemical-Raman combined testing device for non-aqueous system |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3960686B2 (en) * | 1998-06-10 | 2007-08-15 | ロッシュ ディアグノスティクス ゲゼルシャフト ミット ベシュレンクテル ハフツング | Electrochemiluminescence analyzer |
| JP2000346807A (en) * | 1999-06-09 | 2000-12-15 | Hitachi Ltd | Reference electrode for electrochemiluminescent detector |
| CN2567573Y (en) * | 2002-05-20 | 2003-08-20 | 华南师范大学 | Electrochemial luminescence detecting instrument |
| AU2003249350A1 (en) * | 2002-06-20 | 2004-01-06 | Igen International, Inc | Electrochemiluminescence flow cell and flow cell components |
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2006
- 2006-09-30 CN CNB2006100691236A patent/CN100451626C/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106959326A (en) * | 2017-05-19 | 2017-07-18 | 量准（上海）实业有限公司 | Multi-parameter based on saliva property quantitatively detects ovulation device |
| CN107589157A (en) * | 2017-11-01 | 2018-01-16 | 青岛科技大学 | Photic electrochemical sensor |
| CN110579519A (en) * | 2019-09-19 | 2019-12-17 | 常熟理工学院 | electrochemical-controlled rapid COD detection device and method thereof |
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| Publication number | Publication date |
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| CN100451626C (en) | 2009-01-14 |
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Granted publication date: 20090114 Termination date: 20110930 |