CN107238640B - Micro flow cell for use with microdialysis device - Google Patents
Micro flow cell for use with microdialysis device Download PDFInfo
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- CN107238640B CN107238640B CN201710610387.6A CN201710610387A CN107238640B CN 107238640 B CN107238640 B CN 107238640B CN 201710610387 A CN201710610387 A CN 201710610387A CN 107238640 B CN107238640 B CN 107238640B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
- A61B10/0045—Devices for taking samples of body liquids
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/283—Means for supporting or introducing electrochemical probes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
- A61B10/0045—Devices for taking samples of body liquids
- A61B2010/008—Interstitial fluid
Abstract
The invention provides a micro flow cell used with a micro dialysis device, which comprises an upper shell, a lower shell, a base, a reference electrode and a working electrode, wherein the upper shell is provided with a plurality of micro dialysis cells; a through flow cell cavity is arranged at the central axis of the lower shell, an internal thread is arranged at the lower part of the flow cell cavity, a liquid inlet pipe is arranged on the lower shell, and the liquid inlet pipe is communicated with the flow cell cavity; an electrode mounting hole for mounting a reference electrode is vertically formed in the upper part of the upper shell, the bottom of the electrode mounting hole is communicated with the cavity of the flow cell, a liquid outlet pipe is arranged on the upper shell, and the liquid outlet pipe is communicated with the electrode mounting hole; an electrode seat is fixedly arranged on the base. The flow cell is combined with the microdialysis sampling device, so that the real-time monitoring of a sample is realized; the capacity of the flow cell is adjustable, the sample collection time is shortened, and the detection efficiency is greatly improved; the invention can complete the determination of the target object in the shortest time possible, reduce the loss of the target object caused by deterioration and improve the detection accuracy.
Description
Technical Field
The invention relates to a medical analysis and measurement device, in particular to a micro flow cell combined with a microdialysis device.
Background
Microdialysis (MD) is a technique for sampling and analyzing biochemical substances in living extracellular fluid. Because of its unique minimally invasive nature and continuity of sampling, it has been widely used in exploratory tests for various pathophysiological phenomena such as brain tissue, blood, etc., monitoring of neurobiochemistry, and research of drug metabolism. The micro dialysis device mainly comprises a micro dialysis needle (probe), a micro through-flow pump, a sample collector, a quantitative analyzer and the like. During sampling, substances to be detected of extracellular fluid can enter perfusion fluid through a semipermeable membrane along a concentration gradient. Since the flow rate is slow, about 1-10. Mu.L/min, the sample volume is very small. However, the conventional flow cell used in the electrochemical detector today has a capacity of about 10-20 ml, and a large capacity, and if the conventional flow cell is used in combination with a microdialysis device for detection, the conventional flow cell needs to be sampled for 17 hours to 7 days, and the long sampling time causes a lot of defects: on one hand, the conventional flow cell is a non-sealing device, and the overlong sampling time can cause solution volatilization to influence the accuracy of target object concentration detection; on the other hand, acidic, oxidative and reductive substances contained in the surrounding atmosphere may cause degradation, deterioration or interference with the measurement of the target substance. In addition, conventional detection using conventional flow cells is often performed at normal temperature, and the sampling time is too long, and there is a possibility that the target itself may deteriorate and affect the accuracy of the measurement.
At present, a flow cell with good sealing performance is also available, and the commonly used flow cells with good sealing performance are a thin layer flow cell and a multifunctional flow cell. The thin-layer flow cell consists of a base, a flow cell module, a reference electrode, a sample injection tube and a sample outlet tube. The flow cell module comprises 2 blocks, wherein a working electrode (a glassy carbon electrode) is embedded on one block, and an electrode electric signal is led out from a lead on the module. A layer of film with holes is clamped between the two modules, and the size of the holes is the volume of the flow cell. The other module is connected with a reference electrode (white). The two modules are fixed by a metal frame. When the chromatographic column is in operation, the sample inlet of the flow cell is connected with the sample outlet of the chromatographic column, liquid enters the pores of the membrane (namely the flow cell) through the micropores on the module, and flows out from the sample outlet at the upper side after being filled, and the solution is detected by the working electrode. The thin-layer flow cell has the advantages of small capacity, capability of detecting flowing liquid and the like, and is suitable for being used together with a liquid chromatograph, and only detection can be performed because the earlier separation is completed by the chromatograph.
However, the use of such thin-layer flowcells in combination with microdialysis devices has several disadvantages:
(1) The electrode area is too large and the sample size is too small. Cyclic voltammetry and differential pulse methods are commonly used electrochemical detection methods, the detection time of the methods is generally about 5-100 s, for 2 electrodes with the diameter of 5mm in a thin-layer flow device, 100-700 microliters of sample solution is required for the long time, and the capacity of a thin-layer flow cell is only about 1 microliter, so that the detection requirement cannot be met, and the phenomenon that signals are too low or reaction signals cannot be detected is caused.
(2) Electrode polishing and modification are difficult. First, the key to detecting the target by electrochemical method is modification of the electrode. Since the creation of electrochemical methods, researchers have been working on electrode modification methods to expect higher sensitivity, now up to 10 for detection of targets using electrochemical methods -20 M, which is not accessible to bare electrodes. And secondly, a series of pretreatment operations are needed before the electrode is modified, the diameter of the response area of the traditional electrode is generally 2-5 mm, the periphery of the response area is wrapped by polytetrafluoroethylene, and the thickness of the response area is about 2mm, so that the response area of the electrode occupies 25% of the whole electrode area, and the polishing treatment is convenient. While the thin-layer flow cell module has a width of about 2cm and a diameter of about 5mm for the intermediate electrode, the electrode response area is only 4% of the whole module area, and polishing is difficult.
(3) The volume is fixed and not adjustable. The recovery rate of the microdialysis sampling is positively correlated with the concentration of the sample, and the higher the concentration of the sample is, the higher the recovery rate is; the flow rate is inversely related to the perfusion rate, i.e. the faster the perfusion rate, the lower the recovery rate. Therefore, the perfusion rate needs to be adjusted according to the approximate concentration of the sample to be measured, and the proper detection volume is achieved in the shortest time. That is, the optimal detection volume will be different according to the concentration of the sample and the perfusion speed, and the volume of the thin-layer flow cell is the pore of the middle membrane of the two modules, so that the volume is fixed, and the requirement of microdialysis sampling volume change cannot be met.
The multifunctional electrolytic cell consists of a bottom working electrode, a sample cell and a cell cover. The electrolytic cell is characterized in that a working electrode is arranged at the bottom, the electrode height can be adjusted by a screw at the bottom, and the electrode is lifted to adjust the bonding degree between the electrode and the bottom of the sample cell. The positions of the reference electrode and the auxiliary electrode are the same as those of a conventional electrolytic cell, and the electrolytic cell has the advantages that polishing and modification treatment of the counter electrode are convenient, and the electrolytic cell is suitable for being used as an electron microscope characterization sample. It has certain drawbacks such as: the sample cell is too large, so that the method is only suitable for routine analysis; the device has no flow pipe, can not circulate liquid, can not be combined with a microdialysis sampling device, and can only be used as an electrolytic cell; the volume is fixed and cannot be adjusted.
In summary, the conventional electrolytic cells have the problem of fixed capacity, and cannot meet the requirement of microdialysis sampling volume change.
Disclosure of Invention
The invention aims to provide a micro flow cell used with a micro dialysis device, so as to solve the problem that the volume change of the micro dialysis sampling cannot be met due to the fixed capacity in the existing electrolytic cell.
The invention is realized in the following way:
a micro flow cell used with a micro dialysis device comprises an upper shell, a lower shell, a base, a reference electrode and a working electrode; the upper shell is fixedly connected with the lower shell;
a through flow cell cavity is arranged at the central axis of the lower shell, an internal thread is arranged at the lower part of the flow cell cavity, a liquid inlet pipe is arranged on the lower shell, and the liquid inlet pipe is communicated with the flow cell cavity;
an electrode mounting hole for mounting a reference electrode is vertically formed in the upper part of the upper shell, the bottom of the electrode mounting hole is communicated with the cavity of the flow cell, a liquid outlet pipe is arranged on the upper shell, and the liquid outlet pipe is communicated with the electrode mounting hole;
an electrode seat for installing a working electrode is arranged on the base, and external threads matched with the internal threads of the cavity of the flow cell are arranged on the upper part of the working electrode.
The liquid outlet pipe is a platinum liquid outlet pipe.
The base is marked with a dial, and the outer side surface of the electrode seat is provided with a graduated scale.
The electrode plate is arranged at the central axis of the top end of the working electrode, the bottom end of the electrode plate is fixedly connected with a metal rod, a fixing hole is horizontally formed in the lower portion of the metal rod, and the fixing hole is a transverse through hole.
The electrode holder is characterized in that an electrode jack which is vertically penetrated and used for accommodating the insertion of the working electrode is vertically arranged at the central axis of the electrode holder, the bottom end of the electrode jack extends to the upper part of the base, a through hole is horizontally arranged at the top end of the base, the through hole is a transverse through hole, and a fixed plunger is inserted into the through hole.
The electrode mounting hole is a threaded hole, and an external thread matched with the electrode mounting hole is arranged at the bottom end of the reference electrode.
The horizontal section of the metal rod of the working electrode is square.
The electrode jack is a square hole.
The lower part of the base is horizontally provided with a metal wire connected with the working electrode, and one end of the metal wire extends to the bottom end of the electrode jack.
The maximum volume of the cavity of the flow cell is 160 mu L.
The invention comprises an upper shell, a lower shell, a flow cell cavity, a reference electrode and a working electrode. The upper part of the upper shell is vertically provided with an electrode mounting hole for mounting the reference electrode, the upper shell is provided with a liquid outlet pipe, and the upper shell has the functions of sealing the flow cell solution, fixing the reference electrode, leading out the waste solution and the like. The bottom of electrode mounting hole is linked together with the flow cell cavity, and the import of drain pipe is linked together with the electrode mounting hole, and the solution that awaits measuring must link to each other with the reference electrode through the electrode mounting hole earlier, discharges through the drain pipe again, and this design has following advantage: the method can ensure that the reference electrode is always in contact with the solution to be measured in the measuring process, and avoid the problem of separation of the reference electrode and the solution caused by air sealing. The central axis department at the lower casing is provided with the flow cell cavity that link up from top to bottom, and the lower part of flow cell cavity is provided with the internal thread, is provided with the external screw thread that is used for with the internal screw thread complex of flow cell cavity in working electrode's upper portion, and working electrode accessible rotation adjusts its height that rises in the flow cell to adjust the flow cell capacity according to actual need.
The electrode mounting hole is a threaded hole, an external thread matched with the electrode mounting hole is arranged at the bottom end of the reference electrode, and the reference electrode can be fixed on the module through rotation. The liquid outlet pipe is a platinum liquid outlet pipe, the liquid outlet pipe is not easy to oxidize, the liquid outlet pipe is made of inert metal platinum, the liquid outlet pipe not only has the function of leading out liquid, but also can serve as an auxiliary electrode, and the material of the liquid outlet pipe is inert metal, has high overpotential, is not easy to cause oxidation-reduction reaction in the measuring process, and avoids the interference of the material of the flow cell on the measuring process. The electrode mounting hole is a threaded hole, an external thread matched with the electrode mounting hole is arranged at the bottom end of the reference electrode, and the reference electrode can be screwed into the upper shell.
The flow cell is combined with the microdialysis sampling device, so that the real-time monitoring of the sample is realized, the capacity of the flow cell is adjustable, the sample collection time is shortened, and the detection efficiency is greatly improved. The invention can complete the determination of the target object in the shortest time possible, reduce the loss of the target object caused by deterioration, improve the detection accuracy and completely meet the requirement of microdialysis sampling volume change. In addition, the electrode polishing and modification treatment in the structure of the invention is also convenient.
Drawings
Fig. 1 is a schematic structural view of the present invention.
Fig. 2 is a schematic structural view of the upper case.
Fig. 3 is a schematic structural view of the lower case.
Fig. 4 is a schematic structural view of the base.
Fig. 5 is a top view of the base shown in fig. 4.
Fig. 6 is a schematic structural diagram of a reference electrode.
Fig. 7 is a schematic structural view of the working electrode.
Fig. 8 is a schematic view of the structure of the fixed plunger.
In the figure: 1. an upper housing; 1-1, bolt holes; 2. A lower housing; 2-1, a threaded connection hole; 3. a flow cell cavity; 4. a reference electrode; 5. a working electrode; 6. a liquid inlet pipe; 7. a liquid outlet pipe; 8. an electrode mounting hole; 9. an electrode base; 10. a base; 11. penetrating the hole; 12. fixing the plunger; 12-1, clamping a circular ring; 13. an electrode sheet; 14. a dial; 15. a metal rod; 16. a fixing hole; 17. a metal wire; 18. and an electrode jack.
Detailed Description
As shown in fig. 1, the present invention includes an upper case 1, a lower case 2, a base 10, a reference electrode 4, and a working electrode 5. The upper shell 1 is arranged at the top end of the lower shell 2, the upper shell 1 is fixedly connected with the lower shell 2, the upper shell 1 and the lower shell 2 are cylindrical shells, the central axis of the upper shell 1 coincides with the central axis of the lower shell 2, and the outer diameter of the upper shell 1 is equal to the outer diameter of the lower shell 2. The upper shell 1 is uniformly provided with 4 bolt holes 1-1 (figure 2) in the vertical direction, the bolt holes 1-1 are distributed along the same circumference, the upper part of the lower shell 2 is provided with 4 threaded connecting holes 2-1 (figure 3) corresponding to the bolt holes 1-1, the threaded connecting holes 2-1 are threaded blind holes, and the bolt holes 1-1 of the upper shell 1 and the corresponding threaded connecting holes 2-1 of the lower shell 2 are screwed with bolts so as to fixedly connect the upper shell 1 with the lower shell 2.
As shown in fig. 3, a flow cell cavity 3 penetrating up and down is provided at the central axis of the lower housing 2, and the flow cell cavity 3 is used for containing a solution to be measured. The circulation cell cavity 3 is a cylindrical cavity, the inner diameter of the circulation cell cavity is 5mm, an internal thread is arranged at the lower part of the circulation cell cavity 3 and used for screwing the working electrode 5, and the setting height of the working electrode 5 in the circulation cell cavity 3 is adjusted, so that the capacity of the circulation cell cavity 3 is adjusted as required, the maximum volume of the circulation cell cavity 3 is 160 mu L, and the requirement of the sampling amount of the microdialysis device can be met. In fig. 3, a liquid inlet pipe 6 is arranged on the side wall of the lower shell 2, the liquid inlet pipe 6 is communicated with the flow cell cavity 3, the liquid inlet pipe 6 is communicated with the top of the flow cell cavity 3, and the outlet end of the liquid inlet pipe 6 extends out of the side wall of the lower shell 2.
In fig. 2, an electrode mounting hole 8 for mounting the reference electrode 4 is vertically provided at the upper part of the upper housing 1, the electrode mounting hole 8 is a threaded hole, and an external thread matched with the electrode mounting hole 8 is provided at the bottom end of the reference electrode 4. The bottom of electrode mounting hole 8 is linked together with flow cell cavity 3, is provided with drain pipe 7 on last casing 1, and drain pipe 7 is linked together with electrode mounting hole 8, and drain pipe 7 is platinum drain pipe, and drain pipe 7 has the effect of deriving liquid and acting as auxiliary electrode. The drain pipe 7 is linked together with the bottom of electrode mounting hole 8, and the export of drain pipe 7 stretches out in the top surface of last casing 1, and feed liquor pipe 6 and drain pipe 7 distribute in the both sides of the straight line that the central axis of flow cell cavity 3 is located.
As shown in fig. 4 and 5, the base 10 is provided with an electrode holder 9 for mounting the working electrode 5, and the electrode holder 9 and the base 10 are integrally formed. An external thread for matching with the internal thread of the flow cell cavity 3 is arranged on the upper part of the working electrode 5. The base 10 is a flat cylinder, a boss is arranged in the center, and the diameter of the base 10 is larger than the outer diameter of the lower shell 2 so as to ensure the stability of the cavity of the flow cell in the detection process. The top surface at base 10 marks calibrated scale 14, and the range of calibrated scale 14 is 0~20 mu L. The lower shell 2 is provided with scale lines for marking graduations, the outer side surface of the electrode seat 9 is marked with a graduated scale, the graduated scale is vertically arranged, the graduated scale is provided with 8 equally divided big lattices and corresponding numbers, and the length of each big lattice is equal to the pushing length of the electrode seat 9 rotated by the base 10 for one circle.
As shown in fig. 7, an electrode plate 13 is disposed at the central axis of the top end of the working electrode 5, a metal rod 15 is fixedly connected to the bottom end of the electrode plate 13, and the metal rod 15 is a quadrangular prism, i.e. the horizontal section of the metal rod 15 is square. The metal rod 15 is inserted into the electrode holder 9 on the base 10 (fig. 4). A horizontal fixing hole 16 is formed in the lower portion of the metal rod 15, and the fixing hole 16 is a transverse through hole. In fig. 4, an electrode insertion hole 18 penetrating up and down for accommodating insertion of a working electrode is vertically provided at the central axis of the electrode holder 9, and the bottom end of the electrode insertion hole 18 extends to the upper part of the base 10, i.e., the electrode insertion hole 18 penetrates the upper part of the base 10 and the entire electrode holder 9. The electrode jack 18 is a square hole, the side length of the electrode jack 18 is slightly larger than the side length of the horizontal section of the metal rod 15, the square design can enable the working electrode 5 to be highly attached to the electrode jack 18, the problem that the working electrode is easy to shake in the rotating process of the base 10 is avoided, and the working electrode and the electrode are firmly connected. The top end of the base 10 is horizontally provided with a through hole 11, the through hole 11 is a transverse through hole, a fixed plunger 12 is inserted into the through hole 11, the fixed plunger 12 is a cylindrical plunger body, and a structural diagram of the fixed plunger 12 is shown in fig. 8. The head of the fixed plunger 12 is provided with a clamping circular ring 12-1, and the tail end of the fixed plunger 12 sequentially passes through a through hole 11 on the base 10 and a fixing hole 16 of the metal rod 15, wherein the aperture of the through hole 11 is equal to that of the fixing hole 16. A metal wire 17 for connection with a working electrode is horizontally provided at the lower portion of the base 10. One end of the metal wire 17 extends to the bottom end of the electrode insertion hole, so as to be connected with the bottom end of the metal rod 15.
When the invention is used, the modified working electrode 5 is inserted into the electrode jack 18, and the metal wire 17 is connected with the metal rod 15 of the working electrode 5 to draw out the electrode membrane potential. The fixing hole 16 of the metal rod 15 is aligned with the penetration hole 11 of the base 10, and the fixing plunger 12 is inserted to fix the working electrode 5. The base 10 is screwed into the flow cell cavity 3 of the lower shell 2 and is adjusted to the volume required by the solution to be measured, the upper shell 1 is fixedly connected with the lower shell 2 through bolts, and the reference electrode 4 is screwed into the electrode mounting hole 8 of the upper shell 1. The three electrodes are respectively: a reference electrode 4, a working electrode 5, and a liquid outlet pipe 7 as an auxiliary electrode. The solution to be measured enters from the liquid inlet pipe 6 of the lower shell 2, and flows out from the liquid outlet pipe 7 after passing through the reference electrode 4 after filling the flow cell cavity 3 with the solution to be measured. The device may be coupled to a microdialysis device for performing the determination of a sample of the microdialysis solution.
Claims (9)
1. A micro flow cell used with a micro dialysis device is characterized by comprising an upper shell, a lower shell, a base, a reference electrode and a working electrode; the upper shell is fixedly connected with the lower shell;
a through flow cell cavity is arranged at the central axis of the lower shell, an internal thread is arranged at the lower part of the flow cell cavity, a liquid inlet pipe is arranged on the lower shell, and the liquid inlet pipe is communicated with the flow cell cavity;
an electrode mounting hole for mounting a reference electrode is vertically formed in the upper part of the upper shell, the bottom of the electrode mounting hole is communicated with the cavity of the flow cell, a liquid outlet pipe is arranged on the upper shell, and the liquid outlet pipe is communicated with the electrode mounting hole;
an electrode seat for installing a working electrode is arranged on the base, and an external thread matched with the internal thread of the cavity of the flow cell is arranged on the upper part of the working electrode;
the base is marked with a dial, the measuring range of the dial is 0-20 mu L, and the outer side face of the electrode base is provided with a graduated scale.
2. The microdialysis cell according to claim 1 wherein the outlet tube is a platinum outlet tube.
3. The microdialysis cell according to claim 1 wherein an electrode plate is disposed at a central axis of a top end of the working electrode, a metal rod is fixedly connected to a bottom end of the electrode plate, a fixing hole is horizontally formed in a lower portion of the metal rod, and the fixing hole is a transverse through hole.
4. The microdialysis cell according to claim 1 wherein an electrode insertion hole penetrating up and down and used for accommodating insertion of the working electrode is vertically formed in the central axis of the electrode holder, the bottom end of the electrode insertion hole extends to the upper portion of the base, a through hole is horizontally formed in the top end of the base, the through hole is a transverse through hole, and a fixed plunger is inserted into the through hole.
5. The microdialysis cell according to claim 1 wherein the electrode mounting hole is a threaded hole and an external thread is provided at the bottom end of the reference electrode that mates with the electrode mounting hole.
6. A microdialysis cell according to claim 3 wherein the metal rod of the working electrode is square in horizontal cross-section.
7. The microdialysis cell for use with a microdialysis device according to claim 4 wherein the electrode receptacle is a square hole.
8. The microdialysis cell according to claim 4 wherein a metal wire for connection with a working electrode is horizontally disposed at a lower portion of the base, and one end of the metal wire extends to a bottom end of the electrode insertion hole.
9. The microdialysis cell for use with a microdialysis device according to claim 1 wherein the maximum volume of the cell cavity is 160 μl.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710610387.6A CN107238640B (en) | 2017-07-25 | 2017-07-25 | Micro flow cell for use with microdialysis device |
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| CN201710610387.6A CN107238640B (en) | 2017-07-25 | 2017-07-25 | Micro flow cell for use with microdialysis device |
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| CN107238640B true CN107238640B (en) | 2023-07-21 |
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| CN108169302B (en) * | 2017-11-20 | 2020-05-12 | 深圳大学 | Three-electrode system and using method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1358994A (en) * | 2000-12-18 | 2002-07-17 | 中国科学院化学研究所 | Sensor type microdialysis biological living sampling device |
| CN101923067A (en) * | 2010-04-15 | 2010-12-22 | 许建民 | Polarographic flow-through cell |
| CN203720142U (en) * | 2014-03-07 | 2014-07-16 | 中国水产科学研究院 | Miniaturized electrochemical three-electrode system detection pond |
| CN204422464U (en) * | 2014-02-12 | 2015-06-24 | 绿谷（集团）有限公司 | The real time on-line detection device of quick bio signal |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9310330B2 (en) * | 2012-10-12 | 2016-04-12 | Antec Leyden B.V. | Electrochemical flow cell |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1358994A (en) * | 2000-12-18 | 2002-07-17 | 中国科学院化学研究所 | Sensor type microdialysis biological living sampling device |
| CN101923067A (en) * | 2010-04-15 | 2010-12-22 | 许建民 | Polarographic flow-through cell |
| CN204422464U (en) * | 2014-02-12 | 2015-06-24 | 绿谷（集团）有限公司 | The real time on-line detection device of quick bio signal |
| CN203720142U (en) * | 2014-03-07 | 2014-07-16 | 中国水产科学研究院 | Miniaturized electrochemical three-electrode system detection pond |
Non-Patent Citations (1)
| Title |
|---|
| 微透析与现代分析技术联用的研究进展;郑红,石力夫,胡晋红;国外医学.药学分册(第04期);全文 * |
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