CN216717971U - Series negative pressure solid phase extraction device - Google Patents

Abstract

The utility model provides a series negative pressure solid phase extraction device, which structurally comprises a device main body, a filtering extraction bracket, a filtering column, an extraction column, an SPE sleeve and a waste liquid tank, wherein the filtering extraction bracket is arranged on the device main body; the filtering extraction support is arranged at the top of the device main body, and a plurality of pairs of filtering columns and extraction columns are vertically arranged on the filtering extraction support in parallel; a plurality of SPE sleeve pipes are installed on the upper surface of the device body and are connected with the extraction columns in a one-to-one correspondence mode, and the waste liquid groove is formed in the center of the bottom of the inner side of the device body. The utility model has the advantages that: the filter column and the extraction column are connected in series, so that batch treatment in the solid-phase extraction purification process can be realized, a large number of samples can be rapidly detected, manpower is saved, and the working efficiency is greatly improved; adopt circular integrated device structure, the rotatable operation of waste liquid collection system need not to uncap in the use, targets in place in one step, prevents cross contamination, has reduced the target loss of extraction in-process, improves the rate of recovery to improve food safety inspection's accuracy and validity.

Description

Series negative pressure solid phase extraction device

Technical Field

The utility model relates to a solid phase extraction device, in particular to a series negative pressure solid phase extraction device, and belongs to the technical field of food sample detection.

Background

The pretreatment of samples plays an important role in food detection, the existing extraction means comprises liquid-liquid extraction, solid-phase micro-extraction and the like, and frequent solvent transfer and manual operation are not only complicated, but also influence the accuracy of results. Solid phase extraction (SPE for short) is mainly used for separation, purification and concentration of complex matrix samples, aims to reduce matrix interference and improve analysis sensitivity, can improve the recovery rate of analytes compared with the traditional liquid phase extraction method, more effectively separates the analytes from interference components and reduces the sample pretreatment process, and is widely applied to the sample detection process in the fields of medicines, foods, environments, commercial inspection, chemical engineering and the like at present.

Traditional SPE small column is when extracting the very complicated sample of matrix, if contain the sample of high protein high sugar of high grease, need carry out the multistep edulcoration with one or more filtration formula solid phase extraction post, just can use extraction column enrichment extraction after the edulcoration is accomplished, elute again at last, its complex operation, the material is scattered, extraction efficiency is low, although there is some designs to establish ties many solid phase extraction posts or utilized the automatic extraction device, it is not good with malleation or negative pressure device connection, the testing process still relies on researcher manual operation, waste time and energy and cost money. The positive pressure extraction device on the market at present is inconvenient for replacing the extraction column, is easy to cross-contaminate and can not be connected with the extraction column in series; the negative pressure extraction device also has similar problems, the panel interfaces are easy to mutually pollute and can not accurately adjust the pressure, the internal pore diameters of the extraction columns are inconsistent due to the packing process, the pressure is inconsistent, the sample matrix is complex, the extraction speed is natural and asynchronous, after leaching, the cover plate needs to be opened to take out the waste liquid tank, and then the waste liquid tank is put into the test tube for elution, so that the accuracy of the final analysis result is greatly influenced.

Disclosure of Invention

The utility model aims to solve the problems of the existing solid phase extraction equipment and provides a novel negative pressure series solid phase extraction device.

The technical solution of the utility model is as follows: a series negative pressure solid phase extraction device structurally comprises a device body, a filtering extraction support, a filtering column, an extraction column, an SPE sleeve and a waste liquid tank; the filtering extraction support is arranged at the top of the device main body, and a plurality of pairs of filtering columns and extraction columns are vertically arranged on the filtering extraction support in parallel; a plurality of SPE sleeve pipes are installed on the upper surface of the device body and are connected with the extraction columns in a one-to-one correspondence mode, and the waste liquid groove is formed in the center of the bottom of the inner side of the device body.

Furthermore, the whole device body is cylindrical, the top of the device body is provided with an openable top cover, and the side of the device body is provided with a negative pressure air port with a pressure gauge and is connected with an external vacuum pump.

Further, the filtering and extracting support comprises a filtering layer, an extracting layer and an adjustable support; the adjustable support is arranged in the center of a top cover of the device main body, the filtering layer is arranged at the top of the adjustable support, the extraction layer is arranged in the middle of the adjustable support, and the height of the adjustable support can be adjusted and is relatively fixed. The filtering layer and the extraction layer are both disc-shaped with the same size, and the adjustable support penetrates through the circle centers of the filtering layer and the extraction layer at the same time; the filter layer and the extraction layer are radially provided with a plurality of rows of fixing holes in the shape of a circle center, the plurality of filter columns are arranged in the fixing holes of the filter layer, the plurality of extraction columns are correspondingly arranged in the fixing holes of the extraction layer corresponding to the installation positions of the filter columns, and the bottom of each filter column is connected with the top of the corresponding extraction column in series through a connector with an air guide groove.

Further, the SPE sleeve is arranged on the surface of the top cover of the device body. The top of the extraction column is connected with the bottom end of the extraction column through a connector, the middle part of the extraction column is provided with a pressure regulating valve, and the bottom of the extraction column is provided with a pointed liquid outlet.

Further, the waste liquid groove comprises a waste liquid pipe, a liquid discharge pipe, a test tube rack, an adjusting knob and a test tube; the waste liquid pipe is arranged at the top of the waste liquid tank and comprises a main pipe and side pipes which are perpendicular to each other, the main pipe is arranged at the center of the top of the waste liquid tank and is communicated with the interior of the waste liquid tank, and the side pipes extend outwards and horizontally to the bottom of a sharp-end liquid outlet of the SPE sleeve and are correspondingly provided with a corresponding number of liquid receiving ports; the liquid discharge pipe is arranged at the outer side of the bottom of the waste liquid groove and communicated to a waste liquid treatment system outside the device main body; the adjusting knob is arranged on the surface of the top cover at the top of the main pipe and can control the whole waste liquid pipe to rotate around the main pipe, and a waste liquid pipe adjusting valve connected with the waste liquid pipe is arranged at the adjusting knob and is connected with an external vacuum pump; a plurality of rows of test tube racks are sequentially arranged on the side surface of the waste liquid tank, and each row of test tube racks is provided with a plurality of test tubes, and the number of the test tubes corresponds to the position of the SPE sleeve.

Compared with the prior art, the utility model has the advantages that:

1) the filter column and the extraction column are connected in series, so that batch treatment in the solid-phase extraction purification process can be realized, a large number of samples can be rapidly detected, manpower is saved, and the working efficiency is greatly improved;

2) by adopting a circular integrated device structure, the waste liquid collecting system can be operated in a rotating way, and the cover does not need to be opened in the using process, so that the waste liquid collecting system can be put in place in one step;

3) cross contamination is prevented, target substance loss in the extraction process is reduced, the recovery rate is improved, and the accuracy and effectiveness of food safety detection are improved.

Drawings

FIG. 1 is a schematic diagram of the overall structure of a series negative pressure solid phase extraction device of the present invention.

Detailed Description

The technical scheme of the utility model is further explained by combining the attached drawings. Examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are illustrative and intended to be illustrative of the utility model and are not to be construed as limiting the utility model.

In the description of the present invention, it is to be understood that the terms "central," "longitudinal," "lateral," "length," "width," "thickness," "up," "down," "front," "back," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," and the like are used in the orientations and positional relationships indicated in the drawings for convenience of description and simplicity of description, and do not indicate or imply that the device or element so referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be considered as limiting. Furthermore, the terms "first," "second," and the like are used in a tabular sense for descriptive purposes only and are not to be construed as indicating or implying relative importance or imply a number of indicated technical features. Thus, features defined as "first" and "second" may explicitly or implicitly include one or more of the described features. In the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.

In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; may be mechanically connected, may be electrically connected or may be in communication with each other; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.

In the present invention, unless otherwise expressly stated or limited, "above" or "below" a first feature means that the first and second features are in direct contact, or that the first and second features are not in direct contact but are in contact with each other via another feature therebetween. Also, a first feature "on," "above," and "over" a second feature includes that the first feature is directly above and obliquely above the second feature, or simply means that the first feature is at a higher level than the second feature. First feature "under", "below" and "beneath" second feature may include the first feature being directly under and obliquely below the second feature, or merely that the first feature is at a lesser level than the second feature.

The tandem negative pressure solid phase extraction device shown in fig. 1 structurally comprises a device body 1, a filtration extraction bracket 2, a filtration column 3, an extraction column 4, an SPE casing 5 and a waste liquid tank 6; wherein the filtering and extracting bracket 2 is arranged at the top of the device main body 1, and a plurality of pairs of filtering columns 3 and extracting columns 4 are vertically arranged on the filtering and extracting bracket 2 in parallel; a plurality of SPE sleeve pipes 5 are arranged on the upper surface of the device body 1 and are connected with the extraction columns 4 in a one-to-one correspondence mode, and the waste liquid groove 6 is arranged in the center of the bottom of the inner side of the device body 1. (only 2 sets of filter columns and extraction columns and corresponding test tubes are shown in the figure, and multiple sets of filter columns and extraction columns can be arranged according to the actual needs)

The device body 1 is integrally cylindrical, the top of the device body is provided with an openable top cover 1-1, the side surface of the device body is provided with a negative pressure air port 1-2 with a pressure gauge, and the device body is connected with an external vacuum pump and used for realizing a negative pressure environment.

The filtering and extracting bracket 2 comprises a filtering layer 2-1, an extracting layer 2-2 and an adjustable bracket 2-3; the adjustable support 2-3 is arranged at the center of a top cover 1-1 of the device main body 1, the filter layer 2-1 is arranged at the top of the adjustable support 2-3, the extraction layer 2-2 is arranged in the middle of the adjustable support 2-3, the height of the adjustable support 2-3 can be adjusted and is relatively fixed, and the relative heights of the filter layer 2-1 and the extraction layer 2-2 can be respectively set according to requirements.

The filter layer 2-1 and the extraction layer 2-2 are both disc-shaped with the same size, and the adjustable bracket 2-3 simultaneously penetrates through the circle centers of the filter layer 2-1 and the extraction layer 2-2; the surfaces of the filter layer 2-1 and the extraction layer 2-2 are radially provided with a plurality of rows of fixed holes around the circle center, a plurality of filter columns 3 are arranged in the fixed holes of the filter layer 2-1, a plurality of extraction columns 4 are correspondingly arranged in the fixed holes of the extraction layer 2-2 corresponding to the installation positions of the filter columns 3, and the bottom of each filter column 3 is connected with the top of the corresponding extraction column 4 in series through a connector with an air guide groove.

The filter column 3 is filled with a filter material which can be one or more of C18, HLB, silica gel, Florisil and PSA; the extraction column 4 is filled with extraction material, which can be one or more of molecular or ion imprinted polymer and immunoaffinity material.

The SPE sleeve 5 is arranged on the surface of the top cover 1-1 of the device body 1. The top of the extraction column is connected with the bottom end of the extraction column 4 through a connector, and the middle of the extraction column is provided with a pressure regulating valve through which the negative pressure is accurately regulated; the bottom of the liquid outlet is provided with a pointed liquid outlet.

The waste liquid groove 6 comprises a waste liquid pipe 6-1, a liquid discharge pipe 6-2, a test tube rack 6-3, an adjusting knob 6-4 and a test tube 7; the waste liquid pipe 6-1 is arranged at the top of the waste liquid tank 6 and comprises a main pipe and side pipes which are vertical to each other, the main pipe is arranged at the center of the top of the waste liquid tank 6 and is communicated with the interior of the waste liquid tank 6, the side pipes extend outwards and horizontally to the bottom of a pointed liquid outlet of the SPE sleeve 5, and a corresponding number of liquid receiving ports are correspondingly arranged; the liquid discharge pipe 6-2 is arranged at the outer side of the bottom of the waste liquid tank 6 and communicated to a waste liquid treatment system outside the device main body 1; the adjusting knob 6-4 is arranged on the surface of the top cover 1-1 at the top of the main pipe and can control the whole waste liquid pipe 6-1 to rotate around the main pipe, a waste liquid pipe adjusting valve connected with the waste liquid pipe 6-1 is arranged at the position of the adjusting knob 6-4 and is connected with an external vacuum pump, waste liquid collected by the waste liquid pipe 6-1 is pumped out and collected to the waste liquid tank 6, and then is discharged by the liquid discharge pipe 6-2. A plurality of rows of test tube racks 6-3 are sequentially arranged on the side surface of the waste liquid tank 6, and a plurality of test tubes 7 are arranged on each row of test tube racks 6-3, and the number of the test tubes corresponds to the position of the SPE casing 5.

During actual use, the collected samples are added into the filter column 3 in batches, filtered by the filter column 3 and then enter the extraction column 4, and after extraction is finished, the samples are temporarily stored in the SPE casing 5; rotating the waste liquid pipe 6-1 to the position below the SPE sleeve 5 and fixing, carrying out a leaching procedure, collecting the leached waste liquid to a waste liquid tank 6 through the waste liquid pipe 6-1, then rotating the waste liquid pipe 6-1, carrying out an elution procedure again, and collecting the eluent to a test tube 7.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims (6)

1. The utility model provides a series connection negative pressure solid phase extraction device which characterized in that: comprises a device main body (1), a filtration extraction bracket (2), a filtration column (3), an extraction column (4), an SPE casing (5) and a waste liquid tank (6); wherein the filtering and extracting bracket (2) is arranged at the top of the device main body (1), and a plurality of pairs of filtering columns (3) and extracting columns (4) are vertically arranged on the filtering and extracting bracket (2) in parallel; a plurality of SPE sleeves (5) are arranged on the upper surface of the device body (1) and are connected with the extraction columns (4) in a one-to-one correspondence mode, and the waste liquid groove (6) is formed in the center of the bottom of the inner side of the device body (1).

2. The tandem negative pressure solid phase extraction device of claim 1, wherein: the device main body (1) is integrally cylindrical, the top of the device main body is provided with an openable top cover (1-1), the side of the device main body is provided with a negative pressure air port (1-2) with a pressure gauge, and the device main body is connected with an external vacuum pump.

3. The tandem negative pressure solid phase extraction device of claim 1, wherein: the filtering and extracting bracket (2) comprises a filtering layer (2-1), an extracting layer (2-2) and an adjustable bracket (2-3); the adjustable support (2-3) is arranged in the center of a top cover (1-1) of the device main body (1), the filter layer (2-1) is arranged at the top of the adjustable support (2-3), the extraction layer (2-2) is arranged in the middle of the adjustable support (2-3), and the height of the adjustable support (2-3) can be adjusted and is relatively fixed.

4. The tandem negative pressure solid phase extraction apparatus of claim 3, wherein: the filtering layer (2-1) and the extraction layer (2-2) are both disc-shaped and have the same size, and the adjustable support (2-3) simultaneously penetrates through the circle centers of the filtering layer (2-1) and the extraction layer (2-2); the surfaces of the filter layer (2-1) and the extraction layer (2-2) are radially provided with a plurality of rows of fixed holes with the circle centers, a plurality of filter columns (3) are arranged in the fixed holes of the filter layer (2-1), a plurality of extraction columns (4) are correspondingly arranged in the fixed holes of the extraction layer (2-2) corresponding to the installation positions of the filter columns (3), and the bottom of each filter column (3) is connected with the top of the corresponding extraction column (4) in series through a connector with an air guide groove.

5. The tandem negative pressure solid phase extraction device of claim 1, wherein: the SPE sleeve (5) is arranged on the surface of a top cover (1-1) of the device body (1); the top of the extraction column is connected with the bottom end of the extraction column (4) through a connector, the middle part of the extraction column is provided with a pressure regulating valve, and the bottom of the extraction column is provided with a pointed liquid outlet.

6. The tandem negative pressure solid phase extraction device of claim 1, wherein: the waste liquid groove (6) comprises a waste liquid pipe (6-1), a liquid discharge pipe (6-2), a test tube rack (6-3), an adjusting knob (6-4) and a test tube (7); the waste liquid pipe (6-1) is arranged at the top of the waste liquid tank (6) and comprises a main pipe and a side pipe which are perpendicular to each other, the main pipe is arranged at the center of the top of the waste liquid tank (6) and is communicated with the interior of the waste liquid tank (6), the side pipe extends outwards and horizontally to the bottom of a pointed liquid outlet of the SPE sleeve pipe (5), and a corresponding number of liquid receiving ports are correspondingly arranged; the liquid discharge pipe (6-2) is arranged at the outer side of the bottom of the waste liquid tank (6) and communicated to a waste liquid treatment system outside the device main body (1); the adjusting knob (6-4) is arranged on the surface of the top cover (1-1) at the top of the main pipe and can control the whole waste liquid pipe (6-1) to rotate around the main pipe, and a waste liquid pipe adjusting valve connected with the waste liquid pipe (6-1) is arranged at the adjusting knob (6-4) and is connected with an external vacuum pump; a plurality of rows of test tube racks (6-3) are sequentially arranged on the side surface of the waste liquid tank (6), and a plurality of test tubes (7) are arranged on each row of test tube racks (6-3), and the number of the test tubes corresponds to the position of the SPE casing tube (5).

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