CN106954735A - Special yolk solution additive by carrier of peanut shell powder and preparation method thereof - Google Patents
Special yolk solution additive by carrier of peanut shell powder and preparation method thereof Download PDFInfo
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- CN106954735A CN106954735A CN201710139575.5A CN201710139575A CN106954735A CN 106954735 A CN106954735 A CN 106954735A CN 201710139575 A CN201710139575 A CN 201710139575A CN 106954735 A CN106954735 A CN 106954735A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention discloses a kind of low cost preparation method of the special special yolk solution additive of the stichopus japonicus using high adsorption peanut shell powder as carrier, belong to field of feed.It is characterized in that by 80~120 mesh peanut shell powders through freezing swollen stripping technical finesse and by mass percentage, the following components of addition：Yolk liquid 40wt%~70wt% containing special yolk antibody, high adsorption peanut shell powder 30wt%~60wt%, potassium sorbate；The addition of described potassium sorbate is the 0.05%~0.1% of yolk liquid quality.Product of the present invention is well mixed by 1%~5% addition with stichopus japonicus compound feed, is applied in Miao Chizhong, be can be used to prevent and treat stichopus japonicus skin ulcer syndrome or other bacteriosises, and can strengthen stichopus japonicus immunity of organisms.In addition, the production technology of product of the present invention will greatly simplify, production cost is greatly lowered, and is more suitable for large-scale production.
Description
Technical field
The present invention relates to field of feed, more specifically to a kind of using high adsorption peanut shell powder as carrier
Special special yolk solution additive of stichopus japonicus and preparation method thereof.
Background technology
Stichopus japonicus (Apostichopus japonicas) is also known as imitative stichopus japonicus, belong to Echinodermata (Echinodermate),
Holothuroidea (Holothrioider), Stichopodidae (Stichopodidae) imitates stichopus japonicus category (Apostichopus).It is main in China
Originate in the Northern Coastal Regions such as Liaoning Province, Shandong Province.Because of its higher nutritive value and medical value, turn into northern area most at present
One of important rare breed variety, with the continuous expansion of cultivation scale, stichopus japonicus disease problem is on the rise, common at present
Disease mainly includes skin ulcer syndrome, mouth and encloses swelling disease, rotten side disease, rotten stomach trouble and change plate disease etc..Wherein skin ulcer syndrome into
For apostichopus japonicus culture industry it is most common be also endanger most serious disease, seriously govern the sound development of apostichopus japonicus culture industry.
Current apostichopus japonicus culture industry abuse of antibiotics and chemicals situation are universal, and antibiotic and chemicals Reusability hold
Drug resistance is also easy to produce, there is medicament residue, will be stepped down from the stage of history in the today for advocating green agriculture.Yolk antibody has nothing
Poison, noresidue, the advantages of do not develop immunity to drugs, development prospect gets most of the attention.In addition Yolk antibody output capacity is high, and with resistance to
The advantages of heat, good stability, product is widely made to carry out the prevention and treatment of Animal diseases.
Current Yolk antibody product is mostly powdery yolk and dried whole-egg, but in traditional processing technology in need it is dry by freezing
Dry or spray drying, cost is higher.
The content of the invention
It is an object of the invention to simplify Yolk antibody preparation technology, change prepares the conventional method of Yolk antibody product,
Freeze-drying or spray drying technology are not used, but is directly processed high adsorption peanut shell powder parcel yolk liquid,
Naturally crushed after drying, apostichopus japonicus Selenka feed additive is made.The loss of Yolk antibody in freeze-drying process is reduced, while other in egg
Nutritional ingredient obtain effectively utilization, reduce the cost of product.
We attempt selection and prepared using freezing the peanut shell powder after swollen stripping technical finesse as efficient carrier parcel yolk liquid
Feed addictive, save yolk liquid it is freeze-dried or spray drying be dehydrated this process, it is only necessary to be placed in room temperature shady place dry 3
It can obtain finished product within~5 days, make production technology greatly simplified, cost is greatly lowered.
Technical scheme is made up of carrier and special yolk liquid.Carrier is to be obtained after freezing swollen stripping technical finesse
The high adsorption peanut shell powder obtained, special yolk liquid is obtained by the corresponding pathogen Immune Laying Hens of stichopus japonicus bacteriosis.
It is special special the invention provides a kind of stichopus japonicus using high adsorption peanut shell powder as carrier to reach above-mentioned purpose
Property yolk solution additive, including material are prepared from：Special yolk liquid 40wt%~70wt%, high adsorption peanut shell powder
30wt%~60wt%, potassium sorbate；The addition of described potassium sorbate is the 0.05%~0.1% of yolk liquid quality.
Under preferred embodiment, described high adsorption peanut shell powder is as follows using swollen stripping technical finesse, processing method is freezed：
(1) 80-120 mesh drying peanut shell powder is put into micronizer and crushes 10-15min, 200 mesh sieves are crossed after crushing
Net；
(2) its 2-3 times of quality distilled water is added in the peanut shell powder obtained after step (1) ultramicro grinding, and in -20 DEG C
Frost 1-2 days, then at 15-28 DEG C of defrosting after frost；
(3) repeat step (2) 1-3 times, by gained peanut shell powder it is evenly laid out be placed in 15-28 DEG C it is dry 3-5 days, produce
High adsorption peanut shell powder.
The described special special yolk of stichopus japonicus using high adsorption peanut shell powder as carrier is claimed in the present invention simultaneously
The low cost preparation method of solution additive, comprises the following steps：
(1) height is exempted from into egg to be cleaned with clear water, 200-250PPM liquor natrii hypochloritis carries out disinfection 5 minutes, Ran Houyong
Clear water is cleaned, and is dried.The high yolk and egg white for exempting from egg is separated afterwards, takes yolk to squeeze into container, stirs；
(2) yolk is stirred to volumetric expansion, lighter, in thick, is the yolk containing special yolk antibody
Liquid, and weigh quality；
(3) its quality 0.05%-0.1% potassium sorbate is added in the egg yolk liquid obtained to step (2)；
(4) it is the 3/7-3/2 of the yolk liquid quality containing special yolk antibody high adsorption peanut shell powder point is a small amount of more
Secondary addition, is stirred while adding, and high adsorption peanut shell powder is thoroughly mixed with the yolk liquid containing special yolk antibody；
(5) the well mixed yolk liquid containing special yolk antibody is placed in feed granulator with peanut shell powder mixture
In be pressed into a diameter of 2-10mm particles, be placed in indoor shady place and dry naturally, below 10wt% is reduced to moisture；
(6) particle after drying in the shade is placed in separating twice in pulverizer, the particle of crushing is crossed 60-100 eye mesh screens, produced into
Product.
Under preferred embodiment, the height exempts from egg and exempts from egg, anti-Vibrio splindidus height for anti-yellowing extra large Shewanella height to exempt from egg.
Peanut shell powder has unique physique, and its cross section is layer structure, and longitudinal section is honeycombed structure, is had
Larger specific surface area.The present invention, using swollen stripping technology is freezed, is added using this design feature of peanut shell powder in peanut shell powder
Enter distilled water directly to freeze, ice formation ice crystal after the water suction of flaggy gap, volume increase makes its structure crack, thawing occur
Afterwards, cause crack constantly to extend to width and depth direction and form more large fracture, layer structure is disintegrated, specific surface area
Further increase, adsorptivity is further enhanced.Again because peanut shell powder main component is cellulose, stichopus japonicus is had no toxic side effect,
It is aided with the corresponding Yolk antibody of stichopus japonicus pathogen, Yolk antibody is played unique work in terms of stichopus japonicus healthy aquaculture and quick volume increase
With.
The application method of apostichopus japonicus Selenka feed additive of the present invention is：Apostichopus japonicus Selenka feed additive is (1% with quality of the fodder ratio
~5%)：(95%~99%), additive is added in feed and is well mixed, is applied into nursery pond.
The present invention technological innovation be：
(1) peanut shell powder wide material sources, it is cheap；(2) specific IgY in the yolk liquid containing special yolk antibody
The corresponding bacteriosis of stichopus japonicus can be prevented；(3) other nutritional ingredients in special yolk can effectively facilitate stichopus japonicus growth, and
It is remarkably reinforced the immunity of organisms and premunition of extra large stichopus japonicus；(4) antibiotic and chemicals usage amount are reduced；(5) safety non-toxic,
It is easy to use；(6) natural peanut shell powder carrier is solved needs largely to consume energy in conventional Yolk antibody production purification process
The problem in source, greatly reduces cost.
Brief description of the drawings
Fig. 1 is common peanut shell powder cross section,
Fig. 2 is peanut shell powder cross section after the swollen stripping technical finesse of frost,
Fig. 3 is standard IgY concentration curves.
Embodiment
Following non-limiting examples can make one of ordinary skill in the art be more fully understood the present invention, but not with
Any mode limits the present invention.
Test method described in embodiment unless otherwise specified, is conventional method；Unless otherwise specified, the reagent and
Biomaterial, is commercially obtained.
Embodiment 1
The preparation of the special anti-Vibrio splindidus special yolk solution additive of stichopus japonicus using high adsorption peanut shell powder as carrier
Method, comprises the following steps：
(1) 120 mesh drying peanut shell powder are put into micronizer and crush 15min, 200 eye mesh screens are crossed after crushing；
(2) its 2 times of quality distilled water are added in the peanut shell powder obtained after step (1) ultramicro grinding, and in -20 DEG C of ice
Freeze 2 days, then at 15 DEG C of defrostings after frost；
(3) repeat step (2) 3 times, are placed in 15 DEG C of dryings 3 days by gained peanut shell powder is evenly laid out, produce high absorption
Property peanut shell powder.
(4) yolk and egg white that anti-Vibrio splindidus height exempts from egg are separated, takes yolk to squeeze into container, stirred；
(5) egg yolk liquid is stirred to volumetric expansion, lighter in thick, and weighs quality 500g；
(6) 0.25g potassium sorbate is added in the egg yolk liquid obtained to step (2)；
(7) the 3/7 of egg yolk liquid quality high adsorption peanut shell powder 214g is added in six times, stirs while adding, make
High adsorption peanut shell powder is thoroughly mixed with egg liquid；
(8) well mixed egg yolk liquid and peanut shell powder mixture are placed in feed granulator and are pressed into a diameter of 2mm
Particle, is placed in indoor shady place and dries naturally, less than 10% is reduced to moisture；
(9) particle after drying in the shade is placed in separating twice in pulverizer, the particle of crushing is crossed 100 eye mesh screens, got product.
Embodiment 2
The special anti-yellowing extra large Shewanella special yolk solution additive of stichopus japonicus using high adsorption peanut shell powder as carrier
Preparation method, comprises the following steps：
(1) 120 mesh drying peanut shell powder are put into micronizer and crush 15min, 200 eye mesh screens are crossed after crushing；
(2) its 2 times of quality distilled water are added in the peanut shell powder obtained after step (1) ultramicro grinding, and in -20 DEG C of ice
Freeze 2 days, then at 15 DEG C of defrostings after frost；
(3) repeat step (2) 3 times, are placed in 15 DEG C of dryings 3 days by gained peanut shell powder is evenly laid out, produce high absorption
Property peanut shell powder.
(4) yolk and egg white that anti-yellowing extra large Shewanella height exempts from egg are separated, takes yolk to squeeze into container, stirred；
(5) egg yolk liquid is stirred to volumetric expansion, lighter in thick, and weighs quality 500g；
(6) potassium sorbate of addition 0.25g in the egg yolk liquid that step (2) is obtained is thought；
(7) by the 3/2 of egg yolk liquid quality 750g points of 8 additions of high adsorption peanut shell powder, stir while adding, make height
Adsorptivity peanut shell powder is thoroughly mixed with egg yolk liquid；
(8) well mixed egg yolk liquid and peanut shell powder mixture are placed in feed granulator and are pressed into a diameter of 10mm
Particle, is placed in indoor shady place and dries naturally, less than 10% is reduced to moisture；
(9) particle after drying in the shade is placed in separating twice in pulverizer, the particle of crushing is crossed 100 eye mesh screens, got product.
Embodiment 3
IgY contains in the special anti-Vibrio splindidus special yolk solution additive of stichopus japonicus using high adsorption peanut shell powder as carrier
It is fixed to measure
By standard IgY coating buffers doubling dilution into certain gradient (1-0.015625ug/ml), by specific additives sample
Also certain gradient is diluted to, adds 100ul to be coated with 96 hole elisa Plates per hole, 4 DEG C overnight.ELISA Plate is washed with cleaning solution 3 times, per hole
Plus 100ul confining liquids, 37 DEG C incubate after dry, washing 3 times, then add rabbit-anti chicken IgG-HRP 100ul to each hole, 37 DEG C of reactions
1h；Each hole of cleaning solution washing 5 times, adds 100ulTMB substrate solutions, develop the color 20min at room temperature per hole, adds 50ul terminate liquids per hole
Terminating reaction, OD450/630 is surveyed with ELIASA.Standard curve is made to IgY concentration with standard IgY OD values, from standard curve
Check in the concentration of testing sample.Gained standard curve is shown in Fig. 3.For example：Stichopus japonicus using high adsorption peanut shell powder as carrier is special
The anti-actually measured Yolk antibody of Vibrio splindidus special yolk solution additive (embodiment 1) content is 5.083g/kg.
Embodiment 4
The special yolk additive prepared by embodiment 1 is chosen to raise added to stichopus japonicus commodity by 5%, 3% and 1% respectively
In material, gained feed directly feeds stichopus japonicus, and Feeding time is 50 days, and cultivation water bucket volume is 0.05/m3, and cultivation density is 30
Stichopus japonicus/bucket (every stichopus japonicus body weight is 10.64 ± 0.84g).After stichopus japonicus administering transgenic, analysis pair is carried out to its experimental result
Than.
The different additive usage amount stichopus japonicus changes of weight of table 1
| Additive usage amount | Body weight (g)/bucket before experiment | Body weight (g)/bucket after experiment | Daily gain (%) |
| 5% addition | 298 | 456 | 1.06 |
| 3% addition | 304 | 470 | 1.09 |
| 1% addition | 304 | 434 | 0.86 |
| Control | 310 | 436 | 0.81 |
The feeding stichopus japonicus immune indexes contrast of table 2
| Additive usage amount | Cell phagocytic rate (%) | Coelomic fluid lysozyme activity (U/ml) |
| 5% addition | 65 | 49.38±1.47 |
| 3% addition | 60 | 43.74±1.63 |
| 1% addition | 35 | 34.25±1.77 |
| Control | 32 | 32.68±1.27 |
It can be seen that through above example 4 and analysis, apostichopus japonicus Selenka feed additive of the present invention can not only improve sea cucumber life
It is long, moreover it is possible to effectively improve stichopus japonicus immunity and the resistance to pathogen, and benefit is fairly obvious, therefore with before wide application
Scape.
The various methods of table 3 produce IgY preparation Integrated comparatives
From table 3 it can be seen that in the case where not considering the factors such as cost of labor and equipment depreciation, this method early investment
Equipment cost it is minimum, it is even more many that specific energy consumption falls below original 1/5, and significantly, and production process is simple for energy-saving effect,
Flexibly, restraining factors are less, with very big commercial value for the mode of production.
In addition, the present invention is compared to carrier, specifically as shown in Figure 1, 2；Wherein, Fig. 1 is that common peanut shell powder is horizontal
Section, Fig. 2 is peanut shell powder cross section after the swollen stripping technical finesse of present invention frost.
The foregoing is intended to be a preferred embodiment of the present invention, but protection scope of the present invention is not limited thereto,
Any one skilled in the art in the technical scope of present disclosure, technique according to the invention scheme and its
Inventive concept is subject to equivalent or change, should all be included within the scope of the present invention.
Claims (4)
1. a kind of special yolk solution additive using peanut shell powder as carrier, it is characterised in that composition includes：Containing specific ovum
Yolk liquid 40wt%~70wt% of yellow antibody, high adsorption peanut shell powder 30wt%~60wt%, potassium sorbate；Described mountain
The addition of potassium sorbate is the 0.05%~0.1% of yolk liquid quality.
2. the special yolk solution additive as claimed in claim 1 by carrier of peanut shell powder, it is characterised in that described
High adsorption peanut shell powder is as follows using swollen stripping technical finesse, processing method is freezed：
(1) 80~120 mesh drying peanut shell powder are put into 10~15min of crushing in micronizer, 200 mesh sieves is crossed after crushing
Net；
(2) water of its 2~3 times of quality is added in the peanut shell powder obtained after step (1) ultramicro grinding, and 1 is freezed in -20 DEG C
~2 days, then at 15~28 DEG C of defrostings after frost；
(3) step (2) is repeated 1~3 time, is placed in 15~28 DEG C of dryings 3~5 days by gained peanut shell powder is evenly laid out, is produced
High adsorption peanut shell powder.
3. the preparation method of the special yolk solution additive using peanut shell powder as carrier described in claim 2, its feature exists
In comprising the following steps：
(1) height is exempted from into egg to be eluted with water, 200~250ppm liquor natrii hypochloritis carries out disinfection 5 minutes, is then carried out with water
Cleaning, dries, and afterwards separates the high yolk and egg white for exempting from egg, takes yolk to squeeze into container, stir；
(2) yolk is stirred to volumetric expansion, lighter, in thick, produces the yolk liquid containing special yolk antibody, and
Weigh quality；
(3) potassium sorbate of its quality 0.05%~0.1% is added in the yolk liquid obtained to step (2)；
(4) the high adsorption peanut shell powder point for accounting for yolk liquid quality 3/7~3/2 made from step (2) is repeatedly added on a small quantity
Plus, stir while adding, the high adsorption peanut shell powder is thoroughly mixed with the yolk liquid containing special yolk antibody；
(5) the yolk liquid containing special yolk antibody described in will be well mixed is placed in feed granulator with peanut shell powder mixture
In be pressed into a diameter of 2~10mm particles, be placed in indoor shady place and dry naturally, to moisture be reduced to 10wt% with
Under；
(6) particle after drying in the shade is placed in separating twice in pulverizer, the particle of crushing is crossed 60~100 eye mesh screens, got product.
4. the preparation method of the specific shelled egg yellow liquor additive as claimed in claim 3 by carrier of peanut shell powder, its
It is characterised by, height described in step S31 exempts from egg and exempt from egg, anti-Vibrio splindidus height for anti-yellowing extra large Shewanella height to exempt from egg.
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|---|---|---|---|
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| CN107198059A (en) * | 2017-07-20 | 2017-09-26 | 武汉轻工大学 | Egg yolk granule manufacture method, egg yolk granule and aquatic feeds as aquatic feeds |
| CN113040271A (en) * | 2021-02-19 | 2021-06-29 | 苏州大学 | Preparation method and application of yolk antibody feed additive powder |
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| CN113040271A (en) * | 2021-02-19 | 2021-06-29 | 苏州大学 | Preparation method and application of yolk antibody feed additive powder |
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