CN106578733A - Low-cost preparation method of specific hulled whole-egg additive special for apostichopus japonicas and using high-adsorptivity rice husk shell powder as carrier - Google Patents
Low-cost preparation method of specific hulled whole-egg additive special for apostichopus japonicas and using high-adsorptivity rice husk shell powder as carrier Download PDFInfo
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- CN106578733A CN106578733A CN201611185888.6A CN201611185888A CN106578733A CN 106578733 A CN106578733 A CN 106578733A CN 201611185888 A CN201611185888 A CN 201611185888A CN 106578733 A CN106578733 A CN 106578733A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention discloses a low-cost preparation method of a specific hulled whole-egg additive special for apostichopus japonicas and using high-adsorptivityrice husk powder as a carrier, and belongs to the technical field of feeds. The preparation method comprises the following steps of treating 30-60-mesh rice husk powder through a freezing, swelling and stripping technique; besides, adding the following components in percentage by mass: 40-70% of hulled whole eggs containing specific yolk antibodies, 30-60% of high-adsorptivity rice husk powder and 0.05-0.1% of potassium sorbate. The specific hulled whole-egg additive disclosed by the invention is uniformly mixed with a compound feed for apostichopus japonicas in the addition quantity of the specific hulled whole-egg additive being 1-5% so as to obtain a mixture, then the mixture is applied to an apostichopus japonicas fry pond, and the specific hulled whole-egg additive can be used for preventing and treating skin ulcer syndromes or other bacterial diseases of the apostichopus japonicas, and can be used for enhancing the organism immunity of the apostichopus japonicas. In addition, the production technology of the specific hulled whole-egg additive is greatly simplified, the production cost of the specific hulled whole-egg additive is greatly reduced, and the specific hulled whole-egg additive is more suitable for mass production.
Description
Technical field
The invention belongs to field of feed, is related to a kind of new preventing and treating Radix Morinae Bulleyanae skin ulcerative syndrome and other bacillary diseases
The special yolk antibody feed additive and preparation method of disease.
Background technology
Radix Morinae Bulleyanae (Apostichopus japonicas) also known as Apostichopus japonicus, belong to Echinodermata (Echinodermate),
Holothuroidea (Holothrioider), Stichopodidae (Stichopodidae), Apostichopus japonicus category (Apostichopus).It is main in China
Originate in the Northern Coastal Regions such as Liaoning Province, Shandong Province.Because of its higher nutritive value and medical value, northern area is become most at present
One of important famous and precious breed variety, with the continuous expansion of cultivation scale, Radix Morinae Bulleyanae disease problem is on the rise, common at present
Disease mainly includes that skin ulcerative syndrome, mouth enclose swelling disease, rotten side disease, rotten gastropathy and change plate disease etc..Wherein skin ulcerative syndrome into
For the most common disease for being also to endanger most serious of apostichopus japonicus culture industry, the sound development of apostichopus japonicus culture industry is seriously govern.
Apostichopus japonicus culture industry abuse of antibiotics at present and chemicalses situation are universal, and antibiotic and chemicalses Reusability hold
Drug resistance is also easy to produce, there is drug residue, will be stepped down from the stage of history in the today for advocating green agriculture.Yolk antibody has nothing
Poison, noresidue, the advantages of do not develop immunity to drugs, development prospect gets most of the attention.In addition yolk antibody output capacity is high, and has resistance to
The advantages of heat, good stability, widely made product to carry out the prevention and treatment of Animal diseases.
Current yolk antibody product is mostly powdery yolk and dried whole-egg, but in traditional processing technology in need it is dry through freezing
Dry or spray drying, it is relatively costly.Based on considerations above, we attempt selecting to make to freeze the powdered rice hulls after swollen stripping technical finesse
Feed additive is prepared for the efficient carrier parcel shell egg that shells, save the egg liquid that shells it is freeze-dried or be spray-dried dehydration this
Process, it is only necessary to be placed in room temperature shady place and be dried 3-5 days finished product is obtained, makes production technology greatly simplified, and cost is greatly lowered.
The content of the invention
Powdered rice hulls have unique physique, and its cross section is layer structure, with larger specific surface area.The present invention
Using this construction features of powdered rice hulls, using swollen stripping technology is freezed, in powdered rice hulls, distilled water is added directly to freeze, flaggy gap
Ice formation ice crystal after water suction, volume increase make its structure crack occur, after thawing, cause crack constantly to width and depth
Degree direction is extended and forms more large fracture, finally makes layer structure disintegrate, and specific surface area further increases, and adsorptivity further increases
By force.Again as powdered rice hulls main component is cellulose, Radix Morinae Bulleyanae is had no toxic side effect, is aided with the corresponding yolk antibody of Radix Morinae Bulleyanae pathogen,
Yolk antibody is made to play unique effect in terms of Radix Morinae Bulleyanae healthy aquaculture and quick volume increase.
The purpose that the present invention simplifies from yolk antibody preparation technology again, changes the tradition for preparing yolk antibody product
Method, does not use lyophilization or spray drying technology, but high adsorption powdered rice hulls are wrapped up the shell egg that shells directly is carried out
Processing, crushes after drying naturally, makes apostichopus japonicus Selenka feed additive.Damage to yolk antibody in freeze-drying process is not only reduced so
Lose, and also simplify production technology, while other nutritional labelings have also obtained effectively utilization in egg, largely
On the cost for reducing product.
Technical scheme is made up of the carrier and specificity shell egg that shells.Carrier is Jing after freezing swollen stripping technical finesse
The high adsorption powdered rice hulls of acquisition, specificity shell shell egg by the corresponding pathogen Immune Laying Hens acquisition of Radix Morinae Bulleyanae bacterial disease.It is high
The shell mass ratio of shell egg of adsorptivity powdered rice hulls and specificity is 3/7-3/2.
The Radix Morinae Bulleyanae is as follows with different component mass percent in special yolk additive：Going containing special yolk antibody
Shell shell egg 40%-70%, high adsorption powdered rice hulls 30%-60%.The mass percent sum of above-mentioned raw materials is 100%.
As a preferred embodiment, also including potassium sorbate, potassium sorbate addition is above two constituent mass
The 0.05%-0.1% of sum.
Preparation method is：
(1) 30-60 mesh is dried into powdered rice hulls it is put in super micron mill and crush 10-15min, after crushing, crosses 200 eye mesh screens.
(2) add 2-3 times of quality distilled water in the powdered rice hulls obtained after step (1) crushing, and 1-2 is freezed in -20 DEG C
My god, then at thaw at RT after frost；
(3) repeat step (2) 1-3 time, is placed in drying at room temperature 3-5 days by gained powdered rice hulls evenly laid out, obtains final product high suction
Attached property powdered rice hulls；
(4) height is exempted from egg to be cleaned with clear water, 0.5% bromogeramine solutions of Jing immersion 20min dries, then shelled
Squeeze in container, stir；
(5) egg liquid is stirred to into volumetric expansion, lighter in thick, and weighs quality；
(6) repeatedly add the high adsorption powdered rice hulls of the 3/7-3/2 of egg liquid quality point a small amount of, stir while adding, make
High adsorption powdered rice hulls are thoroughly mixed with egg liquid；
(7) egg liquid of mix homogeneously and rice husk powder mixture are placed in feed granulator and are pressed into a diameter of 2-10mm
Grain, is placed in indoor shady place and dries naturally, be reduced to less than 10% to moisture；
(8) granule after drying in the shade is placed in into separating twice in pulverizer, the granule of crushing is crossed 60-100 eye mesh screens, obtained final product into
Product.
The using method of apostichopus japonicus Selenka feed additive of the present invention is：Apostichopus japonicus Selenka feed additive is (1- with quality of the fodder ratio
5%): (95-99%), additive is added to into mix homogeneously in feedstuff, is applied toward nursery pond.
The invention has the advantages that：(1) powdered rice hulls wide material sources, it is cheap；(2) specificity shells the ovum in shell egg
Yellow antibody can prevent the corresponding bacterial disease of Radix Morinae Bulleyanae；(3) other nutritional labelings that specificity shells in shell egg can be effectively facilitated
Radix Morinae Bulleyanae grows, and is remarkably reinforced the immunity of organisms and premunition of Radix Morinae Bulleyanae；(4) antibiotic and chemicalses usage amount are reduced；
(5) safety non-toxic, it is easy to use；(6) natural rice husk powder carrier is needed in solving conventional yolk antibody production purification process
The a large amount of difficult problems for consuming the energy, greatly reduce cost.
1 various methods of table produce IgY preparation Integrated comparatives
As it can be seen from table 1 in the case where the factors such as cost of labor and equipment depreciation are not considered, this method early investment
Equipment cost it is minimum, it is even more many that specific energy consumption falls below original 1/5, and significantly, and production process is simple for energy-saving effect,
Flexibly, restraining factors are less, with very big commercial value for the mode of production.
Description of the drawings
Fig. 1 is common powdered rice hulls cross section；
Fig. 2 is powdered rice hulls cross section after the swollen stripping technical finesse of frost；
Fig. 3 shells IgY assay figures in shell egg additive for the special specificity of Radix Morinae Bulleyanae.
Specific embodiment
Embodiments of the invention are described in detail below in conjunction with technical scheme.
Embodiment 1
The special anti-yellowing extra large Shewanella specificity of Radix Morinae Bulleyanae shells the preparation method of shell egg additive, comprises the steps：
(1) anti-yellowing extra large Shewanella height is exempted from egg to be cleaned with clear water, 0.5% bromogeramine solutions of Jing immersion 20min dries in the air
It is dry, then shelled and squeeze in container, stir；
(2) egg liquid is stirred to into volumetric expansion, lighter in thick, and weighs quality；
(3) repeatedly add the high adsorption powdered rice hulls of the 3/7-3/2 of egg liquid quality point a small amount of, stir while adding, make
High adsorption powdered rice hulls are thoroughly mixed with egg liquid；
(4) egg liquid of mix homogeneously and rice husk powder mixture are placed in feed granulator and are pressed into a diameter of 2-10mm
Grain, is placed in indoor shady place and dries naturally, be reduced to less than 10% to moisture；
(5) granule after drying in the shade is placed in into separating twice in pulverizer, the granule of crushing is crossed 60-100 eye mesh screens, obtained final product into
Product.
Embodiment 2
The special anti-Vibrio splindidus specificity of Radix Morinae Bulleyanae shells the preparation method of shell egg additive, comprises the steps：
(1) anti-Vibrio splindidus height is exempted from egg to be cleaned with clear water, 0.5% bromogeramine solutions of Jing immersion 20min dries,
Shelled again and squeezed in container, stirred；
(2) egg liquid is stirred to into volumetric expansion, lighter in thick, and weighs quality；
(3) repeatedly add the high adsorption powdered rice hulls of the 3/7-3/2 of egg liquid quality point a small amount of, stir while adding, make
High adsorption powdered rice hulls are thoroughly mixed with egg liquid；
(4) egg liquid of mix homogeneously and rice husk powder mixture are placed in feed granulator and are pressed into a diameter of 2-10mm
Grain, is placed in indoor shady place and dries naturally, be reduced to less than 10% to moisture；
(5) granule after drying in the shade is placed in into separating twice in pulverizer, the granule of crushing is crossed 60-100 eye mesh screens, obtained final product into
Product.
Embodiment 3
The special specificity of Radix Morinae Bulleyanae shells IgY assays in shell egg additive
By standard IgY coating buffer doubling dilution into certain gradient (1-0.015625ug/ml), by specific additives sample
Also certain gradient is diluted to, adds per hole 100ul to be coated with 96 hole elisa Plates, 4 DEG C overnight.ELISA Plate 3 times is washed with cleaning mixture, per hole
Plus 100ul confining liquids, dry after 37 DEG C of incubations, wash 3 times, then add rabbit-anti chicken IgG-HRP 100ul, 37 DEG C of reactions to each hole
1h；The each hole of cleaning mixture washing 5 times, adds the 20min that develops the color under 100ulTMB substrate solutions, room temperature per hole, adds 50ul terminate liquids per hole
Terminating reaction, surveys OD with microplate reader450/630.Standard curve is made to IgY concentration with the OD values of standard IgY, is looked into from standard curve
Obtain the concentration of testing sample.
Claims (4)
1. a kind of special specificity of Radix Morinae Bulleyanae with high adsorption powdered rice hulls as carrier shells shell egg additive, it is characterised in that former
Expect that mass percent is：The shell egg 40%-70% that shells containing special yolk antibody, high adsorption powdered rice hulls 30%-60%.
2. a kind of special specificity of Radix Morinae Bulleyanae with high adsorption powdered rice hulls as the carrier according to claim 1 shell egg that shells adds
Plus agent, it is characterised in that using the swollen stripping technical finesse 30-60 height of eye adsorptivity powdered rice hulls of frost, preparation process is as follows：
(1) 30-60 mesh is dried into powdered rice hulls it is put in super micron mill and crush 10-15min, after crushing, crosses 200 eye mesh screens；
(2) add 2-3 times of quality distilled water in the powdered rice hulls obtained after step (1) crushing, and freeze 1-2 days in -20 DEG C, ice
Then at thaw at RT after jelly；
(3) repeat step (2) 1-3 time, is placed in drying at room temperature 3-5 days by gained powdered rice hulls evenly laid out, obtains final product high adsorption
Powdered rice hulls.
3. a kind of special specificity of Radix Morinae Bulleyanae with high adsorption powdered rice hulls as the carrier according to claim 1 shell egg that shells adds
Plus agent, it is characterised in that also including potassium sorbate, potassium sorbate addition is two kinds of constituent mass sums in claim 1
0.05%-0.1%.
4. a kind of low cost preparation method of additive as claimed in claim 1, it is characterised in that comprise the steps：
(1) height is exempted from egg to be cleaned with clear water, 0.5% bromogeramine solutions of Jing immersion 20min dries, then shelled and squeeze into
In container, stirring；
(2) egg liquid is stirred to into volumetric expansion, lighter in thick, and weighs quality；
(3) repeatedly add the high adsorption powdered rice hulls of the 3/7-3/2 of egg liquid quality point a small amount of, stir while adding, make high suction
Attached property powdered rice hulls are thoroughly mixed with egg liquid；
(4) egg liquid of mix homogeneously and rice husk powder mixture are placed in feed granulator and are pressed into a diameter of 2-10mm granules,
Be placed in indoor shady place to dry naturally, less than 10% is reduced to moisture；
(5) granule after drying in the shade is placed in into separating twice in pulverizer, the granule of crushing is crossed 60-100 eye mesh screens, got product.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611185888.6A CN106578733A (en) | 2016-12-20 | 2016-12-20 | Low-cost preparation method of specific hulled whole-egg additive special for apostichopus japonicas and using high-adsorptivity rice husk shell powder as carrier |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611185888.6A CN106578733A (en) | 2016-12-20 | 2016-12-20 | Low-cost preparation method of specific hulled whole-egg additive special for apostichopus japonicas and using high-adsorptivity rice husk shell powder as carrier |
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| CN106578733A true CN106578733A (en) | 2017-04-26 |
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| CN201611185888.6A Pending CN106578733A (en) | 2016-12-20 | 2016-12-20 | Low-cost preparation method of specific hulled whole-egg additive special for apostichopus japonicas and using high-adsorptivity rice husk shell powder as carrier |
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Citations (6)
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| CN102696922A (en) * | 2012-06-14 | 2012-10-03 | 昆山贝瑞康生物科技有限公司 | Intestine protecting compound for sea cucumbers and preparation thereof |
| CN104140100A (en) * | 2014-08-18 | 2014-11-12 | 福建师范大学 | Method for preparing micro-porous activated carbon for gas adsorption by virtue of vacuum freeze drying |
| CN104304678A (en) * | 2014-10-27 | 2015-01-28 | 汉川市缘丰米业有限责任公司 | Methods for preparing fodder medium powder and fodder adsorbent by using rice husk |
| CN105198988A (en) * | 2015-09-30 | 2015-12-30 | 大连理工大学 | Vibrio-splendidus-resistant egg yolk antibody and preparation method thereof |
| CN105831433A (en) * | 2016-03-31 | 2016-08-10 | 中国农业大学 | Grape seed proanthocyanidin additive and preparation method and application thereof |
| CN106071114A (en) * | 2016-05-19 | 2016-11-09 | 河南广安生物科技股份有限公司 | A kind of mixed feed additive of substitute antibiotics and preparation method thereof |
-
2016
- 2016-12-20 CN CN201611185888.6A patent/CN106578733A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102696922A (en) * | 2012-06-14 | 2012-10-03 | 昆山贝瑞康生物科技有限公司 | Intestine protecting compound for sea cucumbers and preparation thereof |
| CN104140100A (en) * | 2014-08-18 | 2014-11-12 | 福建师范大学 | Method for preparing micro-porous activated carbon for gas adsorption by virtue of vacuum freeze drying |
| CN104304678A (en) * | 2014-10-27 | 2015-01-28 | 汉川市缘丰米业有限责任公司 | Methods for preparing fodder medium powder and fodder adsorbent by using rice husk |
| CN105198988A (en) * | 2015-09-30 | 2015-12-30 | 大连理工大学 | Vibrio-splendidus-resistant egg yolk antibody and preparation method thereof |
| CN105831433A (en) * | 2016-03-31 | 2016-08-10 | 中国农业大学 | Grape seed proanthocyanidin additive and preparation method and application thereof |
| CN106071114A (en) * | 2016-05-19 | 2016-11-09 | 河南广安生物科技股份有限公司 | A kind of mixed feed additive of substitute antibiotics and preparation method thereof |
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Application publication date: 20170426 |
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