CN106616041A - Low-cost preparation method of specific whole egg additive special for stichopus japonicas and with high-adsorbability rice hull powder as carrier - Google Patents
Low-cost preparation method of specific whole egg additive special for stichopus japonicas and with high-adsorbability rice hull powder as carrier Download PDFInfo
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- CN106616041A CN106616041A CN201611191171.2A CN201611191171A CN106616041A CN 106616041 A CN106616041 A CN 106616041A CN 201611191171 A CN201611191171 A CN 201611191171A CN 106616041 A CN106616041 A CN 106616041A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention discloses a low-cost preparation method of a specific whole egg additive special for stichopus japonicas and with high-adsorbability rice hull powder as a carrier, belonging to the technical field of feed. 30-to-60-mesh rice hull powder is subjected to freezing expanding-stripping technical processing, and the following components are added in percentage by mass: 40 to 70 percent of shell whole-egg containing a specific egg yolk antibody, 30 to 60 percent of the high-adsorbability rice hull powder and 0.05 to 0.1 percent of potassium sorbate. A product of the method is uniformly mixed with mixed feed of the stichopus japonicas according to addition amount of 1 to 5 percent, is applied in a seedling pond, and can be used to prevent and treat skin ulcer syndrome or other bacteriosis of the stichopus japonicas and enhance the organism immunity of the stichopus japonicas; in addition, a production process of the product can be simplified to a greater extent, the production cost is greatly lowered, and the specific whole egg additive is more suitable for large-scale production.
Description
Technical field
The invention belongs to field of feed, is related to a kind of new preventing and treating stichopus japonicus skin ulcer syndrome and other bacillary diseases
The special yolk antibody feed addictive and preparation method of disease.
Background technology
Stichopus japonicus (Apostichopus japonicas) also known as imitative stichopus japonicus, belong to Echinodermata (Echinodermate),
Holothuroidea (Holothrioider), Stichopodidae (Stichopodidae) imitates stichopus japonicus category (Apostichopus).It is main in China
Originate in the Northern Coastal Regions such as Liaoning Province, Shandong Province.Because of its higher nutritive value and medical value, northern area is become most at present
One of important famous and precious breed variety, with the continuous expansion of cultivation scale, stichopus japonicus disease problem is on the rise, common at present
Disease mainly includes that skin ulcer syndrome, mouth enclose swelling disease, rotten side disease, rotten stomach trouble and change plate disease etc..Wherein skin ulcer syndrome into
For the most common disease for being also to endanger most serious of apostichopus japonicus culture industry, the sound development of apostichopus japonicus culture industry is seriously govern.
Apostichopus japonicus culture industry abuse of antibiotics at present and chemicals situation are universal, and antibiotic and chemicals Reusability hold
Drug resistance is also easy to produce, there is medicament residue, will be stepped down from the stage of history in the today for advocating green agriculture.Yolk antibody has nothing
Poison, noresidue, the advantages of do not develop immunity to drugs, development prospect gets most of the attention.In addition Yolk antibody output capacity is high, and with resistance to
The advantages of heat, good stability, widely made product to carry out the prevention and treatment of Animal diseases.
Current Yolk antibody product is mostly powdery yolk and dried whole-egg, but in traditional processing technology in need it is dry through freezing
Dry or spray drying, it is relatively costly.Based on considerations above, we attempt the powdered rice hulls work after selecting to freeze swollen stripping technical finesse
For efficient carrier parcel shell egg prepare feed addictive, save egg liquid it is freeze-dried or be spray-dried be dehydrated this process, only
Room temperature shady place need to be placed in it is dried 3-5 days and finished product is obtained, make production technology greatly simplified, cost is greatly lowered.
The content of the invention
Powdered rice hulls have unique physique, and its cross section is layer structure, with larger specific surface area.The present invention
Using this design feature of powdered rice hulls, using swollen stripping technology is freezed, distilled water is added directly to freeze in powdered rice hulls, flaggy gap
Ice formation ice crystal after water suction, volume increase makes its structure crack occur, after thawing, causes crack constantly to width and depth
Degree direction is extended and forms more large fracture, finally makes layer structure disintegrate, and specific surface area further increases, and adsorptivity further increases
By force.Again because powdered rice hulls main component is cellulose, stichopus japonicus is had no toxic side effect, is aided with the corresponding Yolk antibody of stichopus japonicus pathogen,
Yolk antibody is set to play unique effect in terms of stichopus japonicus healthy aquaculture and quick volume increase.
The purpose that the present invention simplifies from Yolk antibody preparation technology again, changes the tradition for preparing Yolk antibody product
Method, does not use freeze-drying or spray drying technology, but is directly processed high adsorption powdered rice hulls parcel shell egg,
Naturally crush after drying, make apostichopus japonicus Selenka feed additive.So not only reduce to the loss of Yolk antibody in freeze-drying process, and
And also simplify production technology, while other nutrient contents have also obtained effectively utilization in egg, largely
Reduce the cost of product.
Technical scheme is made up of carrier and specific shell egg.Carrier is to obtain Jing after freezing swollen stripping technical finesse
High adsorption powdered rice hulls, specific shell egg obtains by the corresponding pathogen Immune Laying Hens of stichopus japonicus bacteriosis.High adsorption rice
Shell powder is 3/7-3/2 with the mass ratio of specific shell egg.
The stichopus japonicus is as follows with different component mass percent in special yolk additive：Band containing special yolk antibody
Shell shell egg 40%-70%, powdered rice hulls 30%-60%.The mass percent sum of above-mentioned raw materials is 100%.As one preferably
Embodiment, also include potassium sorbate in said components, potassium sorbate addition is for above two constituent mass sum
0.05%-0.1%.
Preparation method is：
(1) 30-60 mesh is dried into powdered rice hulls it is put into micronizer and crush 10-15min, 200 eye mesh screens is crossed after crushing；
(2) add 2-3 times of quality distilled water in the powdered rice hulls obtained after step (1) crushing, and 1-2 is freezed in -20 DEG C
My god, then at thaw at RT after frost；
(3) repeat step (2) 1-3 time, is placed in drying at room temperature 3-5 days by gained powdered rice hulls are evenly laid out, obtains final product high suction
Attached property powdered rice hulls；
(4) height is exempted from into egg to be cleaned with clear water, the bromogeramine solutions of Jing 0.5% immersion 20min dries, then is shelled
In squeezing into container, stirring；
(5) eggshell for leaving step (4) is cleaned again, dried, and Jing micronizers crush 10-15min, crosses 60-100
Eye mesh screen；
(6) egg liquid is stirred into volumetric expansion, lighter in the egg-shell meal after thick addition ultramicro grinding, and claims
Amount gross mass；
(7) the high adsorption powdered rice hulls of the 3/7-3/2 of shell egg quality point are repeatedly added in egg liquid on a small quantity, when adding
Stirring, makes high adsorption powdered rice hulls be thoroughly mixed with egg liquid；
(8) egg liquid being well mixed and rice husk powder mixture are placed in feed granulator and are pressed into a diameter of 2-10mm
Grain, is placed in indoor shady place and dries naturally, and to moisture less than 10% is reduced to；
(9) particle after drying in the shade is placed in into separating twice in pulverizer, the particle of crushing is crossed 60-100 eye mesh screens, obtained final product into
Product.
The using method of apostichopus japonicus Selenka feed additive of the present invention is：Apostichopus japonicus Selenka feed additive is (1- with quality of the fodder ratio
5) %: (95-99) %, additive is added in feed and is well mixed, apply toward nursery pond.
The invention has the advantages that：(1) powdered rice hulls wide material sources, it is cheap；(2) yolk in specific shell egg resists
Body can prevent the corresponding bacteriosis of stichopus japonicus；(3) other nutrient contents in specific shell egg can effectively facilitate stichopus japonicus growth
(for example：Protein, fat in egg liquid, vitamin, calcareous in egg-shell meal), and it is remarkably reinforced the immunity of organisms of extra large stichopus japonicus
And premunition；(4) antibiotic and chemicals usage amount are reduced；(5) safety non-toxic, it is easy to use；(6) natural powdered rice hulls are carried
Body is solved to be needed to consume a difficult problem for the energy in a large number in conventional Yolk antibody production purification process, greatly reduces cost.
The various methods of table 1 produce IgY preparation Integrated comparatives
As it can be seen from table 1 in the case where the factors such as cost of labor and equipment depreciation are not considered, this method early investment
Equipment cost it is minimum, it is even more many that specific energy consumption falls below original 1/5, and significantly, and production process is simple for energy-saving effect,
Flexibly, restraining factors are less, with very big commercial value for the mode of production.
Description of the drawings
Fig. 1 is common powdered rice hulls cross section；
Fig. 2 is powdered rice hulls cross section after the swollen stripping technical finesse of frost；
Fig. 3 is IgY assay figures in the special specific band shell shell egg additive of stichopus japonicus.
Specific embodiment
Embodiments of the invention are described in detail below in conjunction with technical scheme.
Embodiment 1
The preparation method of the special anti-yellowing extra large Shewanella specificity shell egg additive of stichopus japonicus, comprises the steps：
(1) anti-yellowing extra large Shewanella height is exempted from into egg to be cleaned with clear water, the bromogeramine solutions of Jing 0.5% immersion 20min dries in the air
It is dry, then shelled and squeeze into container, stir；
(2) eggshell for leaving step (1) is cleaned again, dried, and Jing micronizers crush 10-15min, crosses 60-100
Eye mesh screen；
(3) egg liquid is stirred into volumetric expansion, lighter in the egg-shell meal after thick addition ultramicro grinding, and claims
Amount gross mass；
(4) by a small amount of repeatedly addition of the high adsorption powdered rice hulls of the 3/7-3/2 of shell egg quality point, stir while adding, make
High adsorption powdered rice hulls are thoroughly mixed with egg liquid；
(5) egg liquid being well mixed and rice husk powder mixture are placed in feed granulator and are pressed into a diameter of 2-10mm
Grain, is placed in indoor shady place and dries naturally, and to moisture less than 10% is reduced to；
(6) particle after drying in the shade is placed in into separating twice in pulverizer, the particle of crushing is crossed 60-100 eye mesh screens, obtained final product into
Product.
Embodiment 2
The preparation method of the special anti-Vibrio splindidus specificity shell egg additive of stichopus japonicus, comprises the steps：
(1) anti-yellowing extra large Shewanella height is exempted from into egg to be cleaned with clear water, the bromogeramine solutions of Jing 0.5% immersion 20min dries in the air
It is dry, then shelled and squeeze into container, stir；
(2) eggshell for leaving step (1) is cleaned again, dried, and Jing micronizers crush 10-15min, crosses 60-100
Eye mesh screen；
(3) egg liquid is stirred into volumetric expansion, lighter in the egg-shell meal after thick addition ultramicro grinding, and claims
Amount gross mass；
(4) by a small amount of repeatedly addition of the high adsorption powdered rice hulls of the 3/7-3/2 of shell egg quality point, stir while adding, make
High adsorption powdered rice hulls are thoroughly mixed with egg liquid；
(5) egg liquid being well mixed and rice husk powder mixture are placed in feed granulator and are pressed into a diameter of 2-10mm
Grain, is placed in indoor shady place and dries naturally, and to moisture less than 10% is reduced to；
(6) particle after drying in the shade is placed in into separating twice in pulverizer, the particle of crushing is crossed 60-100 eye mesh screens, obtained final product into
Product.
Embodiment 3
IgY assays in the special specific band shell shell egg additive of stichopus japonicus
By standard IgY coating buffer doubling dilution into certain gradient (1-0.015625ug/ml), by specific additives sample
Also certain gradient is diluted to, adds 100ul to be coated with 96 hole elisa Plates per hole, 4 DEG C overnight.ELISA Plate 3 times is washed with cleaning solution, per hole
Plus 100ul confining liquids, dry after 37 DEG C of incubations, wash 3 times, then add rabbit-anti chicken IgG-HRP 100ul, 37 DEG C of reactions to each hole
1h；The each hole of cleaning solution washing 5 times, per hole the 20min that develops the color under 100ulTMB substrate solutions, room temperature is added, and per hole 50ul terminate liquids are added
Terminating reaction, with ELIASA OD is surveyed450/630.Calibration curve is made to IgY concentration with the OD values of standard IgY, is looked into from calibration curve
Obtain the concentration of testing sample.
Claims (4)
1. the special specific shell egg additive of a kind of stichopus japonicus with high adsorption powdered rice hulls as carrier, it is characterised in that raw material and
Mass percent is：Band shell shell egg 40%-70% containing special yolk antibody, high adsorption powdered rice hulls 30%-60%.
2. the special specific shell egg addition of a kind of stichopus japonicus with high adsorption powdered rice hulls as carrier according to claim 1
Agent, it is characterised in that, using the swollen stripping technical finesse 30-60 mesh powdered rice hulls of frost, preparation process is such as the high adsorption powdered rice hulls
Under：
(1) 30-60 mesh is dried into powdered rice hulls it is put into micronizer and crush 10-15min, 200 eye mesh screens is crossed after crushing；
(2) add 2-3 times of quality distilled water in the powdered rice hulls obtained after step (1) crushing, and freeze 1-2 days in -20 DEG C, ice
Then at thaw at RT after jelly；
(3) repeat step (2) 1-3 time, is placed in drying at room temperature 3-5 days by gained powdered rice hulls are evenly laid out, obtains final product high adsorption
Powdered rice hulls.
3. the special specific shell egg addition of a kind of stichopus japonicus with high adsorption powdered rice hulls as carrier according to claim 1
Agent, it is characterised in that also including potassium sorbate, potassium sorbate addition is two kinds of constituent mass sums in claim 1
0.05%-0.1%.
4. the special specific shell egg additive of a kind of stichopus japonicus with high adsorption powdered rice hulls as carrier described in claim 1 it is low
Cost manufacturing processes, it is characterised in that comprise the steps：
(1) height is exempted from into egg to be cleaned with clear water, the bromogeramine solutions of Jing 0.5% immersion 20min dries, then is shelled and squeeze into
In container, stirring；
(2) eggshell for leaving step (1) is cleaned again, dried, and Jing micronizers crush 10-15min, crosses 60-100 mesh sieves
Net；
(3) egg liquid is stirred into volumetric expansion, lighter in the egg-shell meal after thick addition ultramicro grinding, and is weighed total
Quality；
(4) the high adsorption powdered rice hulls of shell egg quality 3/7-3/2 point are repeatedly added in egg liquid on a small quantity, are stirred while adding,
High adsorption powdered rice hulls are made to be thoroughly mixed with egg liquid；
(5) egg liquid being well mixed and high adsorption rice husk powder mixture are placed in feed granulator and are pressed into a diameter of 2-
10mm particles, are placed in indoor shady place and dry naturally, and to moisture less than 10% is reduced to；
(6) particle after drying in the shade is placed in into separating twice in pulverizer, the particle of crushing is crossed 60-100 eye mesh screens, got product.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611191171.2A CN106616041A (en) | 2016-12-20 | 2016-12-20 | Low-cost preparation method of specific whole egg additive special for stichopus japonicas and with high-adsorbability rice hull powder as carrier |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611191171.2A CN106616041A (en) | 2016-12-20 | 2016-12-20 | Low-cost preparation method of specific whole egg additive special for stichopus japonicas and with high-adsorbability rice hull powder as carrier |
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Citations (7)
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|---|---|---|---|---|
| CN102308914A (en) * | 2011-07-25 | 2012-01-11 | 大连赛姆生物工程技术有限公司 | Animal dietotherapy IgY whole egg pulp paste preparation and its preparation method |
| CN102429097A (en) * | 2011-07-25 | 2012-05-02 | 大连赛姆生物工程技术有限公司 | Food therapy yolk antibody yolk liquid pasty preparation for animals and production method thereof |
| CN102696922A (en) * | 2012-06-14 | 2012-10-03 | 昆山贝瑞康生物科技有限公司 | Intestine protecting compound for sea cucumbers and preparation thereof |
| CN104140100A (en) * | 2014-08-18 | 2014-11-12 | 福建师范大学 | Method for preparing micro-porous activated carbon for gas adsorption by virtue of vacuum freeze drying |
| CN104304678A (en) * | 2014-10-27 | 2015-01-28 | 汉川市缘丰米业有限责任公司 | Methods for preparing fodder medium powder and fodder adsorbent by using rice husk |
| CN105198988A (en) * | 2015-09-30 | 2015-12-30 | 大连理工大学 | Vibrio-splendidus-resistant egg yolk antibody and preparation method thereof |
| CN106084280A (en) * | 2016-06-15 | 2016-11-09 | 齐齐哈尔大学 | Utilize the method that low temperature freeze-thaw technology prepares corn porous starch |
-
2016
- 2016-12-20 CN CN201611191171.2A patent/CN106616041A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102308914A (en) * | 2011-07-25 | 2012-01-11 | 大连赛姆生物工程技术有限公司 | Animal dietotherapy IgY whole egg pulp paste preparation and its preparation method |
| CN102429097A (en) * | 2011-07-25 | 2012-05-02 | 大连赛姆生物工程技术有限公司 | Food therapy yolk antibody yolk liquid pasty preparation for animals and production method thereof |
| CN102696922A (en) * | 2012-06-14 | 2012-10-03 | 昆山贝瑞康生物科技有限公司 | Intestine protecting compound for sea cucumbers and preparation thereof |
| CN104140100A (en) * | 2014-08-18 | 2014-11-12 | 福建师范大学 | Method for preparing micro-porous activated carbon for gas adsorption by virtue of vacuum freeze drying |
| CN104304678A (en) * | 2014-10-27 | 2015-01-28 | 汉川市缘丰米业有限责任公司 | Methods for preparing fodder medium powder and fodder adsorbent by using rice husk |
| CN105198988A (en) * | 2015-09-30 | 2015-12-30 | 大连理工大学 | Vibrio-splendidus-resistant egg yolk antibody and preparation method thereof |
| CN106084280A (en) * | 2016-06-15 | 2016-11-09 | 齐齐哈尔大学 | Utilize the method that low temperature freeze-thaw technology prepares corn porous starch |
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Application publication date: 20170510 |