CN106616036A - Low-cost preparation method of specific vitelline fluid additive utilizing high-adsorbability rice hull powder as carrier and specially used for stichopus japonicus - Google Patents
Low-cost preparation method of specific vitelline fluid additive utilizing high-adsorbability rice hull powder as carrier and specially used for stichopus japonicus Download PDFInfo
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- CN106616036A CN106616036A CN201611185380.6A CN201611185380A CN106616036A CN 106616036 A CN106616036 A CN 106616036A CN 201611185380 A CN201611185380 A CN 201611185380A CN 106616036 A CN106616036 A CN 106616036A
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- rice hulls
- powdered rice
- yolk
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/02—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from eggs
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
- C07K2317/23—Immunoglobulins specific features characterized by taxonomic origin from birds
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- General Health & Medical Sciences (AREA)
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Abstract
The invention discloses a low-cost preparation method for a specific vitelline fluid additive utilizing high-adsorbability rice hull powder as a carrier and specially used for stichopus japonicus, belonging to the technical field of feeds. The low-cost preparation method comprises the steps of processing 30-60-mesh rice hull powder by virtue of a freezing-expanding-peeling technique, and adding the following components in percentage by mass: 40%-70% of vitelline fluid containing specific egg yolk antibodies, 30%-60% of high-adsorbability rice hull powder and 0.05%-0.1% of potassium sorbate. 1%-5% of the specific vitelline fluid additive is uniformly mixed with a stichopus japonicas compound feed, and the mixture is applied to a seedling pond, so that the stichopus japonicus skin ulcer syndrome or other bacteriosis can be controlled, and the organism immunity of the stichopus japonicas can be enhanced. Besides, a production process of the specific vitelline fluid additive is greatly simplified, the production cost is greatly lowered, and the low-cost preparation method is more suitable for large-scale production.
Description
Technical field
The invention belongs to field of feed, is related to a kind of new preventing and treating stichopus japonicus skin ulcer syndrome and other bacteriosises
Special yolk feed addictive and preparation method.
Background technology
Stichopus japonicus (Apostichopus japonicas) also known as imitative stichopus japonicus, belong to Echinodermata (Echinodermate),
Holothuroidea (Holothrioider), Stichopodidae (Stichopodidae) imitates stichopus japonicus category (Apostichopus).It is main in China
Originate in the Northern Coastal Regions such as Liaoning Province, Shandong Province.Because of its higher nutritive value and medical value, northern area is become most at present
One of important famous and precious breed variety, with the continuous expansion of cultivation scale, stichopus japonicus disease problem is on the rise, common at present
Disease mainly includes that skin ulcer syndrome, mouth enclose swelling disease, rotten side disease, rotten stomach trouble and change plate disease etc..Wherein skin ulcer syndrome into
For the most common disease for being also to endanger most serious of apostichopus japonicus culture industry, the sound development of apostichopus japonicus culture industry is seriously govern.
Apostichopus japonicus culture industry abuse of antibiotics at present and chemicals situation are universal, and antibiotic and chemicals Reusability hold
Drug resistance is also easy to produce, there is medicament residue, will be stepped down from the stage of history in the today for advocating green agriculture.Yolk antibody has nothing
Poison, noresidue, the advantages of do not develop immunity to drugs, development prospect gets most of the attention.In addition Yolk antibody output capacity is high, and with resistance to
The advantages of heat, good stability, widely made product to carry out the prevention and treatment of Animal diseases.
Current Yolk antibody product is mostly powdery yolk and dried whole-egg, but in traditional processing technology in need it is dry through freezing
Dry or spray drying, it is relatively costly.Based on considerations above, we attempt the powdered rice hulls work after selecting to freeze swollen stripping technical finesse
For efficient carrier parcel yolk prepare feed addictive, save yolk it is freeze-dried or be spray-dried be dehydrated this process, only
Room temperature shady place need to be placed in it is dried 3-5 days and finished product is obtained, make production technology greatly simplified, cost is greatly lowered.
The content of the invention
Powdered rice hulls have unique physique, and its cross section is layer structure, with larger specific surface area.The present invention
Using this design feature of powdered rice hulls, using swollen stripping technology is freezed, distilled water is added directly to freeze in powdered rice hulls, flaggy gap
Ice formation ice crystal after water suction, volume increase makes its structure crack occur, after thawing, causes crack constantly to width and depth
Degree direction is extended and forms more large fracture, finally makes layer structure disintegrate, and specific surface area further increases, and adsorptivity further increases
By force.Again because powdered rice hulls main component is cellulose, stichopus japonicus is had no toxic side effect, is aided with the corresponding Yolk antibody of stichopus japonicus pathogen,
Yolk antibody is set to play unique effect in terms of stichopus japonicus healthy aquaculture and quick volume increase.
The purpose that the present invention simplifies from Yolk antibody preparation technology again, changes the tradition for preparing Yolk antibody product
Method, does not use freeze-drying or spray drying technology, but directly carries out adding by high adsorption powdered rice hulls parcel egg yolk liquid
Work, crushes after drying naturally, makes apostichopus japonicus Selenka feed additive.So not only reduce in freeze-drying process to the damage of Yolk antibody
Lose, and also simplify production technology, while other nutrient contents have also obtained effectively utilization in egg, largely
On the cost for reducing product.
Technical scheme is made up of carrier and special yolk liquid.Carrier is to obtain Jing after freezing swollen stripping technical finesse
The high adsorption powdered rice hulls for obtaining, special yolk liquid is obtained by the corresponding pathogen Immune Laying Hens of stichopus japonicus bacteriosis.Height absorption
Property powdered rice hulls and special yolk liquid mass ratio be 3/7-3/2.
The stichopus japonicus is as follows with different component mass percent in special yolk additive：Ovum containing special yolk antibody
Yellow liquor 40%-70%, high adsorption powdered rice hulls 30%-60%, the mass percent sum of above-mentioned raw materials is 100%, is adopted
Special yolk liquid do not contain egg white liquid.Under preferred embodiment, potassium sorbate is also included in component, potassium sorbate addition is upper
State the 0.05%-0.1% of two kinds of constituent mass sums.
The low cost preparation method of the special special yolk solution additive of stichopus japonicus is：
(1) 30-60 mesh is dried into powdered rice hulls it is put into micronizer and crush 10-15min, 200 eye mesh screens is crossed after crushing；
(2) add 2-3 times of quality distilled water in the powdered rice hulls obtained after step (1) crushing, and 1-2 is freezed in -20 DEG C
My god, then at thaw at RT after frost；
(3) repeat step (2) 1-3 time, is placed in drying at room temperature 3-5 days by gained powdered rice hulls are evenly laid out, obtains final product high suction
Attached property powdered rice hulls；
(4) yolk and egg white that height exempts from egg are separated, takes yolk and squeeze into container, stirred；
(5) egg yolk liquid is stirred into volumetric expansion, lighter in thick, and weighs quality；
(6) by a small amount of repeatedly addition of the high adsorption powdered rice hulls of the 3/7-3/2 of egg yolk liquid quality point, stir while adding,
High adsorption powdered rice hulls are made to be thoroughly mixed with egg yolk liquid；
(7) egg yolk liquid being well mixed and high adsorption rice husk powder mixture are placed in feed granulator and are pressed into diameter
For 2-10mm particles, it is placed in indoor shady place and dries naturally, to moisture less than 10% is reduced to；
(8) particle after drying in the shade is placed in into separating twice in pulverizer, the particle of crushing is crossed 60-100 eye mesh screens, obtained final product into
Product.
The using method of apostichopus japonicus Selenka feed additive of the present invention is：Apostichopus japonicus Selenka feed additive is (1- with quality of the fodder ratio
5) %: (95-99) %, additive is added in feed and is well mixed, apply toward nursery pond.
The invention has the advantages that：(1) powdered rice hulls wide material sources, it is cheap；(2) it is special in special yolk liquid
Property IgY can prevent the corresponding bacteriosis of stichopus japonicus；(3) other nutrient contents in special yolk can effectively facilitate stichopus japonicus life
It is long, and it is remarkably reinforced the immunity of organisms and premunition of extra large stichopus japonicus；(4) antibiotic and chemicals usage amount are reduced；(5) safety
It is nontoxic, it is easy to use；(6) natural rice husk powder carrier is solved in conventional Yolk antibody production purification process needs a large amount of consumption
A difficult problem for the energy, greatly reduces cost.
The various methods of table 1 produce IgY preparation Integrated comparatives
As it can be seen from table 1 in the case where the factors such as cost of labor and equipment depreciation are not considered, this method early investment
Equipment cost it is minimum, it is even more many that specific energy consumption falls below original 1/5, and significantly, and production process is simple for energy-saving effect,
Flexibly, restraining factors are less, with very big commercial value for the mode of production.
Description of the drawings
Fig. 1 is common powdered rice hulls cross section；
Fig. 2 is powdered rice hulls cross section after the swollen stripping technical finesse of frost；
Fig. 3 is IgY assay figures in the special special yolk solution additive of stichopus japonicus.
Specific embodiment
Embodiments of the invention are described in detail below in conjunction with technical scheme
Embodiment 1
The preparation method of the special anti-yellowing extra large Shewanella special yolk solution additive of stichopus japonicus, comprises the steps：
(1) yolk and egg white that anti-yellowing extra large Shewanella height exempts from egg are separated, takes yolk and squeeze into container, stirred；
(2) egg yolk liquid is stirred into volumetric expansion, lighter in thick, and weighs quality；
(3) by a small amount of repeatedly addition of the high adsorption powdered rice hulls of the 3/7-3/2 of egg yolk liquid quality point, stir while adding,
High adsorption powdered rice hulls are made to be thoroughly mixed with egg yolk liquid；
(4) egg yolk liquid being well mixed and rice husk powder mixture are placed in feed granulator and are pressed into a diameter of 2-10mm
Particle, is placed in indoor shady place and dries naturally, and to moisture less than 10% is reduced to；
(5) particle after drying in the shade is placed in into separating twice in pulverizer, the particle of crushing is crossed 60-100 eye mesh screens, obtained final product into
Product.
Embodiment 2
The preparation method of the special anti-Vibrio splindidus special yolk solution additive of stichopus japonicus, comprises the steps：
(1) yolk and egg white that anti-Vibrio splindidus height exempts from egg are separated, takes yolk and squeeze into container, stirred；
(2) egg yolk liquid is stirred into volumetric expansion, lighter in thick, and weighs quality；
(3) by a small amount of repeatedly addition of the high adsorption powdered rice hulls of the 3/7-3/2 of egg yolk liquid quality point, stir while adding,
High adsorption powdered rice hulls are made to be thoroughly mixed with egg yolk liquid；
(4) egg yolk liquid being well mixed and rice husk powder mixture are placed in feed granulator and are pressed into a diameter of 2-10mm
Particle, is placed in indoor shady place and dries naturally, and to moisture less than 10% is reduced to；
(5) particle after drying in the shade is placed in into separating twice in pulverizer, the particle of crushing is crossed 60-100 eye mesh screens, obtained final product into
Product.
Embodiment 3
IgY assays in the special special yolk solution additive of stichopus japonicus
By standard IgY coating buffer doubling dilution into certain gradient (1-0.015625ug/ml), by specific additives sample
Also certain gradient is diluted to, adds 100ul to be coated with 96 hole elisa Plates per hole, 4 DEG C overnight.ELISA Plate 3 times is washed with cleaning solution, per hole
Plus 100ul confining liquids, dry after 37 DEG C of incubations, wash 3 times, then add rabbit-anti chicken IgG-HRP 100ul, 37 DEG C of reactions to each hole
1h；The each hole of cleaning solution washing 5 times, per hole the 20min that develops the color under 100ulTMB substrate solutions, room temperature is added, and per hole 50ul terminate liquids are added
Terminating reaction, with ELIASA OD is surveyed450/630.Calibration curve is made to IgY concentration with the OD values of standard IgY, is looked into from calibration curve
Obtain the concentration of testing sample.
Claims (4)
1. a kind of special special yolk solution additive of stichopus japonicus with high adsorption powdered rice hulls as carrier, it is characterised in that raw material
And mass percent is：Yolk liquid 40%-70% containing special yolk antibody, high adsorption powdered rice hulls 30%-60%, it is above-mentioned
The mass percent sum of raw material is 100%, and the special yolk liquid for being adopted does not contain egg white liquid.
2. the special special yolk solution additive of the stichopus japonicus with high adsorption powdered rice hulls as carrier according to claim 1,
Characterized in that, described high adsorption powdered rice hulls are using the swollen stripping technical finesse 30-60 mesh powdered rice hulls of frost, preparation process is such as
Under：
(1) 30-60 mesh is dried into powdered rice hulls it is put into micronizer and crush 10-15min, 200 eye mesh screens is crossed after crushing；
(2) add 2-3 times of quality distilled water in the powdered rice hulls obtained after step (1) crushing, and freeze 1-2 days in -20 DEG C, ice
Then at thaw at RT after jelly；
(3) repeat step (2) 1-3 time, is placed in drying at room temperature 3-5 days by gained powdered rice hulls are evenly laid out, obtains final product high adsorption
Powdered rice hulls.
3. the special special yolk solution additive of the stichopus japonicus with high adsorption powdered rice hulls as carrier according to claim 1,
Characterized in that, also including potassium sorbate, potassium sorbate addition is the 0.05%- of two kinds of constituent mass sums in claim 1
0.1%.
4. a kind of low cost preparation method of the special special yolk solution additive of stichopus japonicus described in claim 1, including it is as follows
Step：
(1) yolk and egg white that height exempts from egg are separated, takes yolk and squeeze into container, stirred；
(2) egg yolk liquid is stirred into volumetric expansion, lighter in thick, and weighs quality；
(3) by a small amount of repeatedly addition of the high adsorption powdered rice hulls of the 3/7-3/2 of egg yolk liquid quality point, stir while adding, make height
Adsorptivity powdered rice hulls are thoroughly mixed with egg yolk liquid；
(4) egg yolk liquid being well mixed and high adsorption rice husk powder mixture are placed in feed granulator and are pressed into a diameter of 2-
10mm particles, are placed in indoor shady place and dry naturally, and to moisture less than 10% is reduced to；
(5) particle after drying in the shade is placed in into separating twice in pulverizer, the particle of crushing is crossed 60-100 eye mesh screens, got product.
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| CN201611185380.6A CN106616036A (en) | 2016-12-20 | 2016-12-20 | Low-cost preparation method of specific vitelline fluid additive utilizing high-adsorbability rice hull powder as carrier and specially used for stichopus japonicus |
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| CN201611185380.6A CN106616036A (en) | 2016-12-20 | 2016-12-20 | Low-cost preparation method of specific vitelline fluid additive utilizing high-adsorbability rice hull powder as carrier and specially used for stichopus japonicus |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102696922A (en) * | 2012-06-14 | 2012-10-03 | 昆山贝瑞康生物科技有限公司 | Intestine protecting compound for sea cucumbers and preparation thereof |
| CN104140100A (en) * | 2014-08-18 | 2014-11-12 | 福建师范大学 | Method for preparing micro-porous activated carbon for gas adsorption by virtue of vacuum freeze drying |
| CN104304678A (en) * | 2014-10-27 | 2015-01-28 | 汉川市缘丰米业有限责任公司 | Methods for preparing fodder medium powder and fodder adsorbent by using rice husk |
| CN105198988A (en) * | 2015-09-30 | 2015-12-30 | 大连理工大学 | Vibrio-splendidus-resistant egg yolk antibody and preparation method thereof |
-
2016
- 2016-12-20 CN CN201611185380.6A patent/CN106616036A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102696922A (en) * | 2012-06-14 | 2012-10-03 | 昆山贝瑞康生物科技有限公司 | Intestine protecting compound for sea cucumbers and preparation thereof |
| CN104140100A (en) * | 2014-08-18 | 2014-11-12 | 福建师范大学 | Method for preparing micro-porous activated carbon for gas adsorption by virtue of vacuum freeze drying |
| CN104304678A (en) * | 2014-10-27 | 2015-01-28 | 汉川市缘丰米业有限责任公司 | Methods for preparing fodder medium powder and fodder adsorbent by using rice husk |
| CN105198988A (en) * | 2015-09-30 | 2015-12-30 | 大连理工大学 | Vibrio-splendidus-resistant egg yolk antibody and preparation method thereof |
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Application publication date: 20170510 |