CN106900442A - A kind of processing method of rice temp-sensing type dual purpose genic male sterile line facility - Google Patents
A kind of processing method of rice temp-sensing type dual purpose genic male sterile line facility Download PDFInfo
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- CN106900442A CN106900442A CN201710293498.9A CN201710293498A CN106900442A CN 106900442 A CN106900442 A CN 106900442A CN 201710293498 A CN201710293498 A CN 201710293498A CN 106900442 A CN106900442 A CN 106900442A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
- A01G22/22—Rice
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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Abstract
The invention discloses a kind of processing method of rice temp-sensing type dual purpose genic male sterile line facility, after cultivating mud life type regrowth, it is transplanted to treatment pond, control cistern that the temperature in control process pond is reached with the velocity of water circulation in treatment pond by cold water treatment facility, the different cold water treatment times are set by the length of the breeding time of paddy rice.It is applied to rice thermosensitive genie male sterile and adds that for choice breeding success rate 99.98% can be obtained, accelerating selection speed, purification can be reached and bred core seeds, saved the purpose of breeding cost, there is important application value in rice thermosensitive genie male sterile breeding.
Description
Technical field
The present invention adds generation to select, breed the processing method of facility for a kind of paddy rice dual-purpose line with genic sterile, and in particular to paddy rice
The method that Thermo-sensitive dual-purpose genic male sterile line carries out cold water treatment by facility.
Background technology
The Fertility al-teration of rice temp-sensing type dual purpose genic male sterile line is controlled by water temperature, when its ear differentiation was in for 3 ~ 8 phase
It is the sensitive periods of Fertility al-teration, is only in continuous low temperature when sensitive periods（Temperature or water temperature）Reach its critical point temperature of fertility alteration
When value is following, ability self-fertility, on the contrary selfing is shaky.
Rice temp-sensing type dual purpose genic male sterile line seed selection adds generation, seminal propagation, generally relies on local nature cold water resource（Water temperature
Spring, reservoir less than 20 degrees Celsius）, Hainan in Winter, continent autumn, high altitude localities are carried out compared with low temperature season.But the continent autumn
Season, temperature Change was larger, and probably hardly possible reaches the purpose of self-fertility.To enable selfing normal solid, will not seminal propagation interrupt, no
Obtain and do not take the standing grain root and stem of certain plants to Hainan regeneration breeding.Can neither so reach plus generation breeds and consuming fund and breeds coefficient also very
It is low.
Using cold water treatment facility（Refrigeration unit, circulating chilled water pool device, cold water treatment pond）Method, for adding Dai Fan
Educate, accelerated selection Thermo-sensitive dual-purpose genic male sterile line, it is ensured that plus generation selection, breed to follow the prescribed order and carry out as scheduled, accelerate scientific research process,
Shortening the breeding time limit has very strong practical value.
The election effects of country's paddy rice dual purpose genic male sterile line are like a raging fire at present, set using intelligent circulating chilled water treatment pond
Apply development plus generation selection, mating system have no report, only adopted in critical point temperature of fertility alteration Test Identification, core seeds purification
Manually climate box method.
The content of the invention
The purpose of the present invention aims to provide a kind of rice temp-sensing type dual purpose genic male sterile line facility so that plus generation selection, breed
Technology, using the method can ensure that rice temp-sensing type dual purpose genic male sterile line add generation selection, breed to follow the prescribed order and carry out as scheduled, reach
To scientific research process is accelerated, the purpose of experiment funds is saved.
The technical proposal of the invention is realized in this way：
A kind of processing method of rice temp-sensing type dual purpose genic male sterile line facility, it is characterised in that comprise the following steps：
（1）Cultivate mud life type regrowth：The individual plant of the objective trait index for selecting to meet during the infertility is in line with genic sterile,
The neat mud of strain is cut into the root and stem of certain plants and stays stake, stake below 2cm high；
（2）Regrowth is transplanted：Before the phase of regrowth ear differentiation 3, will be implanted in basin or alms bowl with the whole root and stem of certain plants of mud or a point root and stem of certain plants, then contained
The steady root and stem of certain plants 8 ~ 12 days, until more than 1/3rd regrowths are in 4 phase of ear differentiation, starts real after entering to process in pond and topdressing
Apply cold water treatment；
（3）Build cold water treatment facility：Build multiple treatment ponds and cistern and cold water treatment unit is installed, the appearance of cistern
Amount is the twice for processing pond capacity, is connected by water circulating pipe between cistern and treatment pond, by treatment pool surface
Each square metre of product installs a cold water injection tap, and each treatment basin bottom installs a drinking-water pipe；
The water temperature that cold water treatment unit equipment is set is the water temperature of cistern, and cistern passes through water-circulating pipe with the water in treatment pond
Road is fully circulated, and the height for processing the water temperature in pond is adjusted by cistern with treatment pond water circulating speed, and water is followed
Ring speed is faster, and the temperature difference is smaller to each other, otherwise water circulating speed is slower, then the temperature difference is bigger；
After confirming that cold water treatment unit equipment is working properly, when by the length of the breeding time of paddy rice, different cold water treatments are set
Between, short setting cold water treatment time breeding time is shorter, breeding time it is long then conversely, by setting cold water treatment unit equipment
Threshold value adjusts water circulating speed and reaches the purpose of the temperature difference of control process pond and cistern；
（4）Cold water treatment：
A. Temperature Treatment is carried out by the identical different growing type of sterile line：The regrowth that need to be processed is contained in treatment pond,
When in 4 ~ 5 phase of ear differentiation, by 19 degrees Celsius of cistern water temperature, treatment 20 ~ 21 degrees Celsius of sump water temperature be configured and
Regulation and control, kept treatment tank water to be deep to Stem nematode point before the phase of ear differentiation 5, keep the treatment pond depth of water neat after the phase of ear differentiation 6
To the pulvinus position of 2 leaves of stem, continuous processing 14 ~ 21 days, the microscopy pollen transformation situation after the phase of ear differentiation 8, until stem turns
Stopping treatment when can educate or ear, and keep water temperature more than 25 degree；
B. according to paddy rice into jointing stage sequencing be divided into it is precocious, in ripe, ripe type late, be respectively processed：Same ripe
The classification of phase rice young panicle is transplanted and processed in pond at one, Celsius by 19 degrees Celsius of cistern water temperature, treatment sump water temperature 19 ~ 21
Degree is configured and regulates and controls, and precocious process time is 10 ~ 15 days, in ripe process time be 13 ~ 17 days, slow ripe process time is 18
~ 22 days.
In the processing method of this rice temp-sensing type dual purpose genic male sterile line facility of the invention, step（1）The mesh for meeting
Mark shape individual plant be through microscopy abortion thoroughly, the individual plant that bagging self-fertility is 0.
In the processing method of this rice temp-sensing type dual purpose genic male sterile line facility of the invention, step（4）Middle treatment water
There are multiple regrowths children's fringes in pond or between treatment pond, it should which point basin bagging separates from preventing string powder from mixing.
In the processing method of this rice temp-sensing type dual purpose genic male sterile line facility of the invention, step（4）In in young fringe
Broke up for 8 phases, extract fringe apical spikelet pollen microscopic examination, dyeing normally is thought to educate when pollen meets iodine solution.
The solution have the advantages that：It is applied to rice thermosensitive genie male sterile and adds to be obtained for choice breeding success rate
99.98%, accelerating selection speed, purification can be reached and bred core seeds, saved the purpose of breeding cost, in rice thermosensitive genie not
Educate is have important application value in breeding.
Specific embodiment
In order that technological means, creation characteristic, reached purpose and effect that the present invention is realized are easy to understand, enter below
One step illustrates the present invention.
Embodiment 1：Early indica type low-temperature-sensitive line with genic sterile " Qi Yuan S " regeneration pure keeping breeding
（1）The head sowing time in season：The first tenday period of a month in late April to May.
（2）Removal of impurities is rogued：When " Qi Yuan S " is pressed（1）Sowing time is sowed, about early July heading.Note complete to heading from sowing
Colony's economical character Clinical observation on consistency of phase, removes special-shaped strain.
（3）Cut the root and stem of certain plants and stay piling method：Cut Dou Qianmushi composite fertilizers 30kg；Cut the root and stem of certain plants time：Early August, to mid-August, stays stake high
Degree 1cm（I.e. neat mud is smooth to be cut off）, reach regrowth and be mud life type seedling.Cut and to unclog and readjust after the root and stem of certain plants more than rice stub leaf and remove weeds,
Moistening or shallow water management.
（4）Move the root and stem of certain plants and enter pond：About cut 10 days or so after the root and stem of certain plants, the i.e. removable root and stem of certain plants to basin alms bowl enters pond or direct when regrowth fully occurs
Pond cultivation is implanted into, is applied fertilizer in right amount in pond.
（5）Cold water treatment：10 days or so or enter 10 or so behind pond after the root and stem of certain plants is cut（Now most of stem ear differentiation is in 4
Phase）Come into effect cold water treatment.Process time is with water temperature is controlled：With 19 ~ 20 degrees Celsius of water temperature continuous processings 15 days, the depth of water was neat
The 2 leaf pulvinus that fall to stem are defined.
Embodiment 2：In the regeneration of indica type low-temperature-sensitive line with genic sterile " emerging 1539S " plus generation selection.
（1）The head sowing time in season：The first tenday period of a month in late April to May.
（2）Removal of impurities is rogued：When " emerging 1539S " is pressed（1）Sowing time is sowed, about late July heading.Note from sowing to heading
Economical character Clinical observation on consistency in each strain of full period, the strain of selection tool objective trait is used to regenerate plus generation.
（3）Cut the root and stem of certain plants and stay piling method：Dou Qianmushi composite fertilizers 30kg is cut to elected strain；Cut the root and stem of certain plants time：Mid-August is to August
The last ten-days period, stubble height 1cm（I.e. neat mud is smooth to be cut off）, reach regrowth and be mud life type seedling.Leaf more than rice stub is unclogged and readjusted after cutting the root and stem of certain plants
And remove weeds, moistening or shallow water management.
（4）Move the root and stem of certain plants and enter pond：About cut 10 days or so after the root and stem of certain plants, the i.e. removable root and stem of certain plants to basin alms bowl enters pond or direct when regrowth fully occurs
Pond cultivation is implanted into, is applied fertilizer in right amount in pond.
（5）Cold water treatment：10 days or so or enter 15 or so behind pond after the root and stem of certain plants is cut（Now most of stem ear differentiation is in 4
Phase）Come into effect cold water treatment.Process time is with water temperature is controlled：With 20 ~ 21 degrees Celsius of water temperature continuous processings 15 days, the depth of water was neat
The 2 leaf pulvinus that fall to stem are defined.
Above example is only to of the invention for example, those skilled in the art can be to being retouched
The embodiment stated is made the modification of different modes or supplement or is replaced using similar mode, but it is defined not to deviate the present invention
Scope.
Claims (4)
1. a kind of processing method of rice temp-sensing type dual purpose genic male sterile line facility, it is characterised in that comprise the following steps：
（1）Cultivate mud life type regrowth：The individual plant of objective trait index for meeting is selected during line with genic sterile is in infertility, will
The neat mud of strain cuts the root and stem of certain plants and stays stake, stake below 2cm high；
（2）Regrowth is transplanted：Before the phase of regrowth ear differentiation 3, will be implanted in basin or alms bowl with the whole root and stem of certain plants of mud or a point root and stem of certain plants, then contained
The steady root and stem of certain plants 8 ~ 12 days, until more than 1/3rd regrowths are in 4 phase of ear differentiation, starts real after entering to process in pond and topdressing
Apply cold water treatment；
（3）Build cold water treatment facility：Build multiple treatment ponds and cistern and cold water treatment unit is installed, the appearance of cistern
Amount is the twice for processing pond capacity, is connected by water circulating pipe between cistern and treatment pond, by treatment pool surface
Each square metre of product installs a cold water injection tap, and each treatment basin bottom installs a drinking-water pipe；
The water temperature that cold water treatment unit equipment is set is the water temperature of cistern, and cistern passes through water-circulating pipe with the water in treatment pond
Road is fully circulated, and the height for processing the water temperature in pond is adjusted by cistern with treatment pond water circulating speed, and water is followed
Ring speed is faster, and the temperature difference is smaller to each other, otherwise water circulating speed is slower, then the temperature difference is bigger；
After confirming that cold water treatment unit equipment is working properly, when by the length of the breeding time of paddy rice, different cold water treatments are set
Between, short setting cold water treatment time breeding time is shorter, breeding time it is long then conversely, by setting cold water treatment unit equipment
Threshold value adjusts water circulating speed and reaches the purpose of the temperature difference of control process pond and cistern；
（4）Cold water treatment：
A. Temperature Treatment is carried out by the identical different growing type of sterile line：The regrowth that need to be processed is contained in treatment pond,
When in 4 ~ 5 phase of ear differentiation, by 19 degrees Celsius of cistern water temperature, treatment 20 ~ 21 degrees Celsius of sump water temperature be configured and
Regulation and control, kept treatment tank water to be deep to Stem nematode point before the phase of ear differentiation 5, keep the treatment pond depth of water neat after the phase of ear differentiation 6
To the pulvinus position of 2 leaves of stem, continuous processing 14 ~ 21 days, the microscopy pollen transformation situation after the phase of ear differentiation 8, until stem turns
Stopping treatment when can educate or ear, and keep water temperature more than 25 degree；
B. according to paddy rice into jointing stage sequencing be divided into it is precocious, in ripe, ripe type late, be respectively processed：Same ripe
The classification of phase rice young panicle is transplanted and processed in pond at one, Celsius by 19 degrees Celsius of cistern water temperature, treatment sump water temperature 19 ~ 21
Degree is configured and regulates and controls, and precocious process time is 10 ~ 15 days, in ripe process time be 13 ~ 17 days, slow ripe process time is 18
~ 22 days.
2. the processing method of rice temp-sensing type dual purpose genic male sterile line facility according to claim 1, it is characterised in that step
（1）The individual plant of the target shape for meeting be through microscopy abortion thoroughly, the individual plant that bagging self-fertility is 0.
3. the processing method of rice temp-sensing type dual purpose genic male sterile line facility according to claim 1, it is characterised in that step
（4）There are multiple regrowths children's fringes in middle treatment pond or between treatment pond, it should which point basin bagging separates from preventing from going here and there powder
Mix.
4. the processing method of rice temp-sensing type dual purpose genic male sterile line facility according to claim 1, it is characterised in that step
（4）In in the phase of ear differentiation 8, extract fringe apical spikelet pollen microscopic examination, dyeing normally is thought to educate when pollen meets iodine solution.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710293498.9A CN106900442A (en) | 2017-04-28 | 2017-04-28 | A kind of processing method of rice temp-sensing type dual purpose genic male sterile line facility |
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| CN201710293498.9A CN106900442A (en) | 2017-04-28 | 2017-04-28 | A kind of processing method of rice temp-sensing type dual purpose genic male sterile line facility |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2591956Y (en) * | 2002-11-16 | 2003-12-17 | 茂名市两系杂交水稻研究发展中心 | Rice low-temperature-sensitive two-purpose nuclear sterile line breeding and identifying temperature-controlling and irrigating apparatus |
| CN102067796A (en) * | 2010-10-18 | 2011-05-25 | 曾翔 | New method for regulating and controlling water temperature in breeding process of low temperature sensitive sterile line of paddy |
| CN203027841U (en) * | 2012-12-21 | 2013-07-03 | 浙江之豇种业有限责任公司 | Cold water irrigation system for cultivating bilinear photo-thermo sensitive genic male sterile line of paddy rice |
| CN104396647A (en) * | 2014-10-31 | 2015-03-11 | 湖南农丰种业有限公司 | Production method for core seeds in photo-thermo sensitive sterile line of rice |
| CN105532429A (en) * | 2015-12-11 | 2016-05-04 | 合肥丰乐种业股份有限公司 | Method for breeding thermo-photoperiod sensitive rice utility type dual-purpose genic male sterile line |
| JP2017023031A (en) * | 2015-07-21 | 2017-02-02 | 株式会社水稲生産技術研究所 | Cultivation method for hybrid rice seed production |
-
2017
- 2017-04-28 CN CN201710293498.9A patent/CN106900442A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2591956Y (en) * | 2002-11-16 | 2003-12-17 | 茂名市两系杂交水稻研究发展中心 | Rice low-temperature-sensitive two-purpose nuclear sterile line breeding and identifying temperature-controlling and irrigating apparatus |
| CN102067796A (en) * | 2010-10-18 | 2011-05-25 | 曾翔 | New method for regulating and controlling water temperature in breeding process of low temperature sensitive sterile line of paddy |
| CN203027841U (en) * | 2012-12-21 | 2013-07-03 | 浙江之豇种业有限责任公司 | Cold water irrigation system for cultivating bilinear photo-thermo sensitive genic male sterile line of paddy rice |
| CN104396647A (en) * | 2014-10-31 | 2015-03-11 | 湖南农丰种业有限公司 | Production method for core seeds in photo-thermo sensitive sterile line of rice |
| JP2017023031A (en) * | 2015-07-21 | 2017-02-02 | 株式会社水稲生産技術研究所 | Cultivation method for hybrid rice seed production |
| CN105532429A (en) * | 2015-12-11 | 2016-05-04 | 合肥丰乐种业股份有限公司 | Method for breeding thermo-photoperiod sensitive rice utility type dual-purpose genic male sterile line |
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Application publication date: 20170630 |
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