CN106880565B - A kind of plant extracts and its preparation method and application for the light spot of skin-whitening - Google Patents
A kind of plant extracts and its preparation method and application for the light spot of skin-whitening Download PDFInfo
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- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
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Abstract
The present invention relates to a kind of plant extracts and its preparation method and application for the light spot of skin-whitening, the plant extracts is raw material by using rice fermentation liquid, european verbena leaf extracting solution, aloe leaf extract, radix sophorae extracting solution and allantoin and carries out being suitble to weight proportion, the plant extracts being prepared tyrosinase inhibitory activity with higher, tyrosinase inhibition rate are up to 82.41%；Have effects that promote skin whiteness and brightness, lighten the stain, can be without side-effects compared with being used on the light spot of skin-whitening, it can be used as the Whitening, spot functional additive of skin care item, be with a wide range of applications.
Description
Technical field
The invention belongs to technical field of skin care, and in particular to a kind of plant extracts for the light spot of skin-whitening and its
Preparation method and application.
Background technique
Color spot, that is, cutaneous pigmentation, medical name are dyschromicum skin disease, lead to color in skin by a variety of causes
Element increases and can not be metabolized in time and cause melanin pigmentation and be unevenly distributed, and performance is the stigmas such as black, yellowish-brown on the skin
Point.
Color spot occurs in epidermis, because temporarily or caused by permanent epidermis melanocyte dysfunction.Epidermis is by base
Bottom is developed, and basal layer is made of basal cell and melanocyte, and melanocyte generates melanin, and basal cell is not
It is divided disconnectedly, generates neoblast.Melanocyte is one of important composition cell of skin, positioned at the melanocyte of basal layer of epidermis
Cell origin is the basis of pigment synthesis in human body pigmentary system in neural crest.In dendrite, the melanin that is formed into the cell
It is transported in corneocyte by dendrite.It is constituted an epidermis unit with 1:36 ratio and keratinocyte, is deposited
It is that basal layer of epidermis, melanocyte form skin color, while absorbable ultraviolet light by synthesis nucleic, make body from purple
The damage of outside line.The depth of skin color is that how much decisions of melanocyte quantity are synthesized by melanocyte.
To prevent the basic principle of pigment deposition to be embodied in following several respects: first, inhibit the generation of melanin.Pass through suppression
The generation of tyrosinase processed and the activity of tyrosinase, or the intermediate of interference melanin production, to prevent pigmented spots
Melanin generation.Second, the reduction of melanin, photooxidation prevent.By the abatement of horn cell stimulation melanin, make
Generated melanin desalination.Third promotes the metabolism of melanin.By improving the metabolism of skin, keep melanin rapid
It is discharged outside skin.4th, prevent the entrance of ultraviolet light.By there is the preparation of sun-proof result, stops ultraviolet light with physical method, prevent
Only there is ultraviolet light to form excessive melanin.
It can thus be seen that the inhibition main path of melanocyte is the inhibition intracellular of melanocyte and the extracellular inhibition of melanocyte.
In addition, the variation of skin color can be measured using skin complexion measuring instrument.The amount generallyd use at present
The measurement method for changing skin color is the colorimeter system as defined in International Commission on Illumination (CIE) (Lab colorimeter system) measurement skin
The variation of skin color, method quantization is more accurate, can reflect the variation of skin color Spatial Multi-Dimensional, the quantization of the colour of skin is made more may be used
It leans on.By quantify and naked eyes it cannot be seen that slight change also can reflect out.Test index L*, a*, b* are three-dimensional right angle
The coordinate value of coordinate system, wherein L value characterization L* is white balance, and value is bigger, and color is more biased to white, conversely, being biased to black；
ITA ° of value is skin subjects type angle, is the numerical value of characterization skin brightness relevant to L* and b*.ITA ° of value is bigger, and skin is got over
It is bright, conversely, skin is more obscure.
Both at home and abroad all there are some problems in Skin whitening care cosmetics at present, and such as universal whitening effect is poor, and the shield that whitening effect is good
Skin product often contain quinhydrones and mercury salt again, and toxic side effect is big, and long-time service will cause skin and permanently decolourize and contact dermatitis.Cause
This there is an urgent need to develop it is some it is pure natural, Whitening, spot effect is good, whitening plant extracts without side-effects or Small side effects is multiple
Side.
Summary of the invention
In order to solve the above problems existing in the present technology, the present invention provides a kind of plants for the light spot of skin-whitening
Extract and its preparation method and application.The Whitening, spot effect of the plant extracts is good, without side-effects, can be used as skin care item
Whitening, spot functional additive, be with a wide range of applications.
The technical scheme adopted by the invention is as follows:
A kind of plant extracts for the light spot of skin-whitening, raw material components include:
Rice fermentation liquid, 5-15 parts by weight；
European verbena leaf extracting solution, 3-7 parts by weight；
Aloe leaf extract, 5-15 parts by weight；
Radix sophorae extracting solution, 3-7 parts by weight；
Allantoin, 15-25 parts by weight.
The rice fermentation liquid is prepared with the following method:
(A1) rice is taken, after soaking in water, is cooked, shortening rice is obtained；
(A2) aspergillus oryzae is inoculated with into shortening rice described in step (A1), the inoculation quality of the aspergillus oryzae accounts for described ripe
The 1-3% of rice milling meal quality, carried out under conditions of temperature is 35-37 DEG C, humidity is 90-95% later cultivate 6-9 days, through from
The heart, filtering are to get the rice fermentation liquid.
The european verbena leaf extracting solution is prepared with the following method:
(B1) european verbena leaf is taken, through drying, crushes, obtains european verbena leaf fine powder；
(B2) ethyl alcohol is added in the european verbena leaf fine powder in step (B1) for the first time, and carries out reflux at 80-85 DEG C and mentions
1-2h is taken, centrifugation, filtering obtain the first filtrate and the first filter residue；Ethyl alcohol is added and in 70- second into first filter residue
80 DEG C carry out heat preservation and extract 2-4h, and centrifugation, filtering obtain the second filtrate and the second filter residue；By first filtrate and the second filter
Liquid merges, and concentration carries out micro porous filtration after removing ethyl alcohol to get the european verbena leaf extracting solution.
In step (B1), the partial size of the european verbena leaf fine powder is 90-110 mesh；
In step (B2), the volumetric concentration for adding ethyl alcohol twice is 70-90%；The quality of the european verbena leaf fine powder with
The ratio between the volume of addition ethyl alcohol is 1:15-1:20g/ml, the quality of first filter residue and addition ethyl alcohol for the second time for the first time
The ratio between volume is 1:15-1:20g/ml；
The concentration is that water-bath is concentrated, and the temperature of the concentration is 35-45 DEG C, carries out the filter sizes of the micro porous filtration
It is 0.4-0.5 μm.
The aloe leaf extract is prepared with the following method:
(C1) Chinese aloe leaf is taken, smashes and is slurried after peeling, obtains aloe slurries；
(C2) ethyl alcohol is added in the aloe slurries in step (C1), after impregnating 20-40min, centrifugation, filtering, consolidate
Shape object；Distilled water is added for the first time into the solid content, after impregnating 20-40min, heat preservation is carried out at 90-100 DEG C and extracts 1-
2h, centrifugation, filtering, obtains the first filtrate and the first filter residue；Distilled water is added and in 90- second into first filter residue
100 DEG C carry out heat preservation and extract 1-2h, and centrifugation, filtering obtain the second filtrate and the second filter residue；By first filtrate and the second filter
Liquid merges, and concentration carries out micro porous filtration after removing distilled water to get the aloe leaf extract.
In step (C2), the mass ratio of the ethyl alcohol and the aloe slurries is 1.5:1-2.5:1, the volume of the ethyl alcohol
Concentration is 70-90%；
The solid content and the mass ratio for adding distilled water for the first time are 1:8-1:12；First filter residue with add for the second time
The mass ratio for adding distilled water is 1:8-1:12；
The concentration is that water-bath is concentrated, and the temperature of the concentration is 85-95 DEG C, carries out the filter sizes of the micro porous filtration
It is 0.4-0.5 μm.
The radix sophorae extracting solution is prepared with the following method:
(D1) radix sophorae is taken, through drying, crushes, obtains radix sophorae fine powder；
(D2) distilled water is added in the radix sophorae fine powder in step (D1) for the first time, after impregnating 20-40min, in 90-95
DEG C carry out refluxing extraction 1-2h, centrifugation, filtering, obtain the first filtrate and the first filter residue；Add for second into first filter residue
Enter distilled water and carry out heat preservation at 90-95 DEG C and extract 1-2h, centrifugation, filtering obtain the second filtrate and the second filter residue；By described
One filtrate merges with the second filtrate, and concentration carries out micro porous filtration after removing distilled water to get the radix sophorae extracting solution.
In step (D1), the partial size of the radix sophorae fine powder is 35-45 mesh；
In step (D2), the radix sophorae fine powder and the mass ratio for adding distilled water for the first time are 1:18-1:22；Described
The mass ratio of one filter residue and second of addition distilled water is 1:18-1:22；
The concentration is that water-bath is concentrated, and the temperature of the concentration is 85-95 DEG C, carries out the filter sizes of the micro porous filtration
It is 0.4-0.5 μm.
The preparation method of the plant extracts for the light spot of skin-whitening, specific steps are as follows:
The rice fermentation liquid, european verbena leaf extracting solution, aloe leaf extract and radix sophorae extracting solution are taken respectively and are carried out
After mixing, allantoin is added, is sufficiently mixed uniformly to get the plant extracts.
Application of the plant extracts in the skin care item that preparation is used for the light spot of skin-whitening.
The invention has the benefit that
Plant extracts of the present invention for the light spot of skin-whitening, by using rice fermentation liquid, european verbena leaf
Extracting solution, aloe leaf extract, radix sophorae extracting solution and allantoin are raw material and carry out being suitble to weight proportion, the plant being prepared
Object extract tyrosinase inhibitory activity with higher, tyrosinase inhibition rate are up to 82.41%；With promotion skin whiteness
With brightness, lighten the stain the effect of, can be compared with the light spot of skin-whitening be used on, without side-effects, the whitening that can be used as skin care item is light
Spot functional additive, is with a wide range of applications.
Specific embodiment
To make the object, technical solutions and advantages of the present invention clearer, technical solution of the present invention will be carried out below
Detailed description.Obviously, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Base
Embodiment in the present invention, those of ordinary skill in the art are obtained all without making creative work
Other embodiment belongs to the range that the present invention is protected.
1mg is represented in following example with 1 parts by weight.
Embodiment 1
The present embodiment provides a kind of plant extracts for the light spot of skin-whitening, raw material components include:
Rice fermentation liquid, 5 parts by weight；
European verbena leaf extracting solution, 7 parts by weight；
Aloe leaf extract, 5 parts by weight；
Radix sophorae extracting solution, 7 parts by weight；
Allantoin (offer of Shanghai Lu Ya Biotechnology Co., Ltd), 35 parts by weight.
Further, the preparation method of the plant extracts for the light spot of skin-whitening, the specific steps are as follows:
(1) rice fermentation liquid is prepared
(A1) rice is taken, is added after 1 times of quality water carries out immersion 10h, is cooked, obtain shortening rice；
(A2) aspergillus oryzae (Japanese brewery industry Co., Ltd. provides), institute are inoculated with into shortening rice described in step (A1)
The inoculation quality for stating aspergillus oryzae accounts for the 1% of the shortening rice quality, later under conditions of temperature is 35 DEG C, humidity is 90%
Cultivate 6 days, is centrifuged, is filtered to get the rice fermentation liquid；
(2) european verbena leaf extracting solution is prepared
(B1) european verbena leaf is taken, through drying, is crushed, obtaining partial size is 90 mesh european verbena leaf fine powders；
(B2) it is 70% ethyl alcohol that volumetric concentration is added in the european verbena leaf fine powder in step (B1) for the first time, and controls institute
The ratio between volume of the quality and addition ethyl alcohol of stating european verbena leaf fine powder is 1:15g/ml, later in 80 DEG C of progress refluxing extraction 2h,
Centrifugation, filtering, obtain the first filtrate and the first filter residue；
It is 70% ethyl alcohol that into first filter residue, second, which is added volumetric concentration, and controls the quality of first filter residue
The ratio between volume with second of addition ethyl alcohol is 1:15g/ml, carries out heat preservation at 70 DEG C later and extracts 4h, centrifugation, filtering obtain
Second filtrate and the second filter residue；
First filtrate is merged with the second filtrate, concentration is carried out under 35 DEG C of water bath conditions and removes ethyl alcohol concentration, later
It is that 0.4 μm of filter membrane carries out micro porous filtration to get the european verbena leaf extracting solution with aperture；
(3) aloe leaf extract is prepared
(C1) Chinese aloe leaf, ultraviolet-sterilization after peeling are taken, and smashes and is slurried, obtains aloe slurries；
(C2) volumetric concentration is added in the aloe slurries in step (C1) is 70% ethyl alcohol, and controls the ethyl alcohol and institute
The mass ratio for stating aloe slurries is 1.5:1, and after impregnating 20min, centrifugation, filtering obtain solid content；
Distilled water is added for the first time into the solid content and controls the solid content and is with the mass ratio for adding distilled water
1:8 after impregnating 20min, heat preservation is carried out at 90 DEG C and extracts 2h, centrifugation, filtering obtain the first filtrate and the first filter residue；
Into first filter residue distilled water is added and controls first filter residue and second of addition distilled water for second
Mass ratio be 1:8, carried out later at 90 DEG C heat preservation extract 2h, centrifugation, filtering, obtain the second filtrate and the second filter residue；
First filtrate is merged with the second filtrate, concentration is carried out under 85 DEG C of water bath conditions and removes distilled water, Zhi Houyong
Aperture is that 0.4 μm of filter membrane carries out micro porous filtration to get the aloe leaf extract；
(4) radix sophorae extracting solution is prepared
(D1) radix sophorae is taken, through drying, is crushed, the radix sophorae fine powder that partial size is 35 mesh is obtained；
(D2) distilled water is added in the radix sophorae fine powder in step (D1) for the first time and control the radix sophorae fine powder with
The mass ratio of addition distilled water is 1:18 for the first time, and after impregnating 20min, in 90 DEG C of progress refluxing extraction 2h, centrifugation, filtering are obtained
To the first filtrate and the first filter residue；
Into first filter residue distilled water is added and controls first filter residue and second of addition distilled water for second
Mass ratio be 1:18, carried out later at 90 DEG C heat preservation extract 2h, centrifugation, filtering, obtain the second filtrate and the second filter residue；
First filtrate is merged with the second filtrate, concentration is carried out under 85 DEG C of water bath conditions and removes distilled water, Zhi Houyong
Aperture is that 0.4 μm of filter membrane carries out micro porous filtration to get the radix sophorae extracting solution；
(5) the rice fermentation liquid, european verbena leaf extracting solution, aloe leaf extract and radix sophorae extracting solution is taken to go forward side by side respectively
After row mixing, allantoin is added, is sufficiently mixed uniformly to get the plant extracts.
Embodiment 2
The present embodiment provides a kind of plant extracts for the light spot of skin-whitening, raw material components include:
Rice fermentation liquid, 15 parts by weight；
European verbena leaf extracting solution, 3 parts by weight；
Aloe leaf extract, 15 parts by weight；
Radix sophorae extracting solution, 3 parts by weight；
Allantoin, 25 parts by weight.
Further, the preparation method of the plant extracts for the light spot of skin-whitening, the specific steps are as follows:
(1) rice fermentation liquid is prepared
(A1) rice is taken, is added after 2 times of quality water carry out immersion 15h, is cooked, obtain shortening rice；
(A2) aspergillus oryzae is inoculated with into shortening rice described in step (A1), the inoculation quality of the aspergillus oryzae accounts for described ripe
The 3% of rice milling meal quality carries out cultivating 9 days later under conditions of temperature is 37 DEG C, humidity is 95%, is centrifuged, is filtered, i.e.,
Obtain the rice fermentation liquid；
(2) european verbena leaf extracting solution is prepared
(B1) european verbena leaf is taken, through drying, is crushed, obtaining partial size is 110 mesh european verbena leaf fine powders；
(B2) it is 90% ethyl alcohol that volumetric concentration is added in the european verbena leaf fine powder in step (B1) for the first time, and controls institute
The ratio between volume of the quality and addition ethyl alcohol of stating european verbena leaf fine powder is 1:20g/ml, later in 85 DEG C of progress refluxing extraction 1h,
Centrifugation, filtering, obtain the first filtrate and the first filter residue；
It is 90% ethyl alcohol that into first filter residue, second, which is added volumetric concentration, and controls the quality of first filter residue
The ratio between volume with second of addition ethyl alcohol is 1:20g/ml, carries out heat preservation at 80 DEG C later and extracts 2h, centrifugation, filtering obtain
Second filtrate and the second filter residue；
First filtrate is merged with the second filtrate, concentration is carried out under 45 DEG C of water bath conditions and removes ethyl alcohol concentration, later
It is that 0.5 μm of filter membrane carries out micro porous filtration to get the european verbena leaf extracting solution with aperture；
(3) aloe leaf extract is prepared
(C1) Chinese aloe leaf, ultraviolet-sterilization after peeling are taken, and smashes and is slurried, obtains aloe slurries；
(C2) volumetric concentration is added in the aloe slurries in step (C1) is 90% ethyl alcohol, and controls the ethyl alcohol and institute
The mass ratio for stating aloe slurries is 2.5:1, and after impregnating 40min, centrifugation, filtering obtain solid content；
Distilled water is added for the first time into the solid content and controls the solid content and is with the mass ratio for adding distilled water
1:12 after impregnating 40min, heat preservation is carried out at 100 DEG C and extracts 1h, centrifugation, filtering obtain the first filtrate and the first filter residue；
Into first filter residue distilled water is added and controls first filter residue and second of addition distilled water for second
Mass ratio be 1:12, carried out later at 100 DEG C heat preservation extract 1h, centrifugation, filtering, obtain the second filtrate and the second filter residue；
First filtrate is merged with the second filtrate, concentration is carried out under 95 DEG C of water bath conditions and removes distilled water, Zhi Houyong
Aperture is that 0.5 μm of filter membrane carries out micro porous filtration to get the aloe leaf extract；
(4) radix sophorae extracting solution is prepared
(D1) radix sophorae is taken, through drying, is crushed, the radix sophorae fine powder that partial size is 45 mesh is obtained；
(D2) distilled water is added in the radix sophorae fine powder in step (D1) for the first time and control the radix sophorae fine powder with
The mass ratio of addition distilled water is 1:22 for the first time, and after impregnating 40min, in 95 DEG C of progress refluxing extraction 1h, centrifugation, filtering are obtained
To the first filtrate and the first filter residue；
Into first filter residue distilled water is added and controls first filter residue and second of addition distilled water for second
Mass ratio be 1:22, carried out later at 95 DEG C heat preservation extract 1h, centrifugation, filtering, obtain the second filtrate and the second filter residue；
First filtrate is merged with the second filtrate, concentration is carried out under 95 DEG C of water bath conditions and removes distilled water, Zhi Houyong
Aperture is that 0.5 μm of filter membrane carries out micro porous filtration to get the radix sophorae extracting solution；
(5) the rice fermentation liquid, european verbena leaf extracting solution, aloe leaf extract and radix sophorae extracting solution is taken to go forward side by side respectively
After row mixing, allantoin is added, is sufficiently mixed uniformly to get the plant extracts.
Embodiment 3
The present embodiment provides a kind of plant extracts for the light spot of skin-whitening, raw material components include:
Rice fermentation liquid, 10 parts by weight；
European verbena leaf extracting solution, 5 parts by weight；
Aloe leaf extract, 10 parts by weight；
Radix sophorae extracting solution, 5 parts by weight；
Allantoin, 20 parts by weight.
Further, the preparation method of the plant extracts for the light spot of skin-whitening, the specific steps are as follows:
(1) rice fermentation liquid is prepared
(A1) rice is taken, is added after 1.5 times of quality water carry out immersion 12h, is cooked, obtain shortening rice；
(A2) aspergillus oryzae is inoculated with into shortening rice described in step (A1), the inoculation quality of the aspergillus oryzae accounts for described ripe
The 2% of rice milling meal quality carries out cultivating 7 days later under conditions of temperature is 36 DEG C, humidity is 93%, is centrifuged, is filtered, i.e.,
Obtain the rice fermentation liquid；
(2) european verbena leaf extracting solution is prepared
(B1) european verbena leaf is taken, through drying, is crushed, obtaining partial size is 100 mesh european verbena leaf fine powders；
(B2) it is 80% ethyl alcohol that volumetric concentration is added in the european verbena leaf fine powder in step (B1) for the first time, and controls institute
The ratio between the quality of european verbena leaf fine powder and the volume of addition ethyl alcohol are stated for 1:18g/ml, later in 82 DEG C of progress refluxing extractions
1.5h, centrifugation, filtering, obtains the first filtrate and the first filter residue；
It is 80% ethyl alcohol that into first filter residue, second, which is added volumetric concentration, and controls the quality of first filter residue
The ratio between volume with second of addition ethyl alcohol is 1:18g/ml, carries out heat preservation at 75 DEG C later and extracts 3h, centrifugation, filtering obtain
Second filtrate and the second filter residue；
First filtrate is merged with the second filtrate, concentration is carried out under 40 DEG C of water bath conditions and removes ethyl alcohol concentration, later
It is that 0.45 μm of filter membrane carries out micro porous filtration to get the european verbena leaf extracting solution with aperture；
(3) aloe leaf extract is prepared
(C1) Chinese aloe leaf, ultraviolet-sterilization after peeling are taken, and smashes and is slurried, obtains aloe slurries；
(C2) volumetric concentration is added in the aloe slurries in step (C1) is 80% ethyl alcohol, and controls the ethyl alcohol and institute
The mass ratio for stating aloe slurries is 2:1, and after impregnating 30min, centrifugation, filtering obtain solid content；
Distilled water is added for the first time into the solid content and controls the solid content and is with the mass ratio for adding distilled water
1:10 after impregnating 30min, heat preservation is carried out at 95 DEG C and extracts 1.5h, centrifugation, filtering obtain the first filtrate and the first filter residue；
Into first filter residue distilled water is added and controls first filter residue and second of addition distilled water for second
Mass ratio be 1:10, carried out later at 95 DEG C heat preservation extract 1.5h, centrifugation, filtering, obtain the second filtrate and the second filter residue；
First filtrate is merged with the second filtrate, concentration is carried out under 90 DEG C of water bath conditions and removes distilled water, Zhi Houyong
Aperture is that 0.45 μm of filter membrane carries out micro porous filtration to get the aloe leaf extract；
(4) radix sophorae extracting solution is prepared
(D1) radix sophorae is taken, through drying, is crushed, the radix sophorae fine powder that partial size is 40 mesh is obtained；
(D2) distilled water is added in the radix sophorae fine powder in step (D1) for the first time and control the radix sophorae fine powder with
The mass ratio of addition distilled water is 1:20 for the first time, after impregnating 30min, in 92 DEG C of progress refluxing extraction 1.5h, is centrifuged, filtering,
Obtain the first filtrate and the first filter residue；
Into first filter residue distilled water is added and controls first filter residue and second of addition distilled water for second
Mass ratio be 1:20, carried out later at 92 DEG C heat preservation extract 1.5h, centrifugation, filtering, obtain the second filtrate and the second filter residue；
First filtrate is merged with the second filtrate, concentration is carried out under 90 DEG C of water bath conditions and removes distilled water, Zhi Houyong
Aperture is that 0.45 μm of filter membrane carries out micro porous filtration to get the radix sophorae extracting solution；
(5) the rice fermentation liquid, european verbena leaf extracting solution, aloe leaf extract and radix sophorae extracting solution is taken to go forward side by side respectively
After row mixing, allantoin is added, is sufficiently mixed uniformly to get the plant extracts.
Experimental example
1, prepared by laboratory sample
Using basic components (carbomer 0.5wt%, hydroxyethyl cellulose 0.1wt%, propylene glycol 10wt%, glycerol
5wt%, glyceryl acrylate/acrylic copolymer and Gantrez AN-119 10wt%, sodium hydroxide 0.15wt%, Metagin
Ester 0.1wt%, deionized water surplus) be used as carrier, respectively by rice fermentation liquid, european verbena leaf extracting solution, aloe leaf extract,
After radix sophorae extracting solution, allantoin (one-component) and plant extracts described in embodiment 3 are compounded with basic components
Sample liquid A, sample is made in (mass fraction that wherein one-component or the plant extracts account for basic components is 2wt%)
Liquid B, sample liquid C, sample liquid D, sample liquid E, sample liquid F carry out tyrosinase inhibition rate, coloration (L value), skin respectively later
The measurement of melanin MI value (light spot effect).
2, volunteer when carrying out coloration (L value) and the detection of dermal melanin MI value requires:
Women's health subject of 30 ages at 30 years old~40 years old, average age 34 years old.Skin photo-biological type is III
Or IV type can strictly observe requirement of experiment based on indoor activity, and sign volunteer informed consent form person.
Exclusion criteria: recipient site has acute inflammation, immune deficiency or other skins patient；Allergic constitution has cosmetics
Allergies person；Nearly 2 months internal reference dosing object clinical trial persons or other cosmetic industries estimator；Gestation and breast feeding women；6
Received any type of skin treating within a month, may be exposed under ultraviolet light for a long time during test and (such as travel, spend a holiday) causes
Solarization increases；Recipient site application anti-inflammatory drug during test, whole body application glucocorticoid and immunosuppressor during test
Class drug；Requirement of experiment cannot be strictly observed, experimenter is cooperated.
Rejecting standard: gestation, adverse reaction, lost to follow-up and other special circumstances require to exit experimenter in experimentation.
One, tyrosinase inhibition rate measures
The levodopa for preparing 1.5g/L using the phosphate buffer of 0.01mol/L, pH=6.8 takes 40 μ l to add as substrate
To after 96 orifice plates, 80 μ l phosphate buffers and 40 μ l sample liquids are added.Then the 100U/ml tyrosinase phosphoric acid that 40 μ l are added is slow
Fliud flushing reacts 30min in 30 DEG C of water-baths after mixing, measure the absorbance at 490nm.Every group sets 3 multiple holes, calculates tyrosine
Enzyme inhibition activity, calculation formula is following, and (C1 is enzyme in formula is not added sample；C2 is not enzyme to be not added sample；The enzyme sample-adding product of T1；T2 is not
Enzyme sample-adding product):
Inhibiting rate=[1- (AT1-AT2)/(AC1-AC2)] × 100%
Measurement result is as shown in table 1.
The measurement of table 1- difference sample liquid tyrosinase inhibition rate
From table 1 it follows that although rice fermentation liquid, european verbena leaf extracting solution, aloe leaf extract, radix sophorae extract
Liquid, allantoin (sample liquid A-E) have certain inhibitory effect to tyrosinase, but above-mentioned each component is carried out compound rear shape
At the tyrosinase inhibition rate of plant extracts (sample liquid F) of the present invention greatly promote, up to 82.41%.
Two, the measurement of coloration (L value)
L value is white balance, and value is bigger, and color is more biased to white, conversely, being biased to black.Sample liquid A is used on the left of cheek
(basic components+account for the sample liquid that basic components 2wt% rice ferment filtrate is made into), cheek right side (are matched on basis using sample liquid F
Side+account for the sample liquid that 3 gained plant extracts of basic components 2wt% embodiment is made into), measurement result is as shown in table 2.
The L value of table 2- sample liquid A and sample liquid F measure
As can be seen from Table 2, for the test section within experimental period using sample liquid A, F relative to skin background, L value is bright
Aobvious to improve, two kinds of sample liquids have the effect for promoting skin whiteness, and sample liquid E is significantly higher than sample liquid A, i.e., in experimental period
The interior sample liquid F containing plant extracts described in embodiment 3 has good effect to skin whiteness is promoted, and is higher than and contains only
A kind of sample liquid A whitening effect of rice ferment filtrate.
Three, the measurement of dermal melanin MI value (light spot effect)
MI value characterizes melanin index, and numberical range is 0~999, and numerical value is bigger, and dermal melanin content is higher.In will
Using using sample liquid A, (basic components+account for basic components 2wt% rice ferment filtrate are made at Yuan Zhe face color spot or spot
Sample liquid), using sample liquid F, (basic components+account for 3 gained plant extracts of basic components 2wt% embodiment are matched on the right side of cheek
At sample liquid), measurement result is as shown in table 3.
The measurement of the MI value of table 3- sample liquid A and sample liquid F
As can be seen from Table 3, after using sample liquid A and sample liquid F, the melanin index of spot is below basis at two
Value, two kinds of sample liquids have fading effect to spot, and sample liquid F is significantly higher than sample liquid A, i.e., contain within experimental period
There is the sample liquid F of plant extracts described in embodiment 3 that there is good effect to Kohakuhi (color spot), and is substantially higher in only
The light spot effect of sample liquid A containing rice ferment filtrate a kind of.
The above description is merely a specific embodiment, but scope of protection of the present invention is not limited thereto, any
Those familiar with the art in the technical scope disclosed by the present invention, can easily think of the change or the replacement, and should all contain
Lid is within protection scope of the present invention.Therefore, protection scope of the present invention should be based on the protection scope of the described claims.
Claims (9)
1. a kind of plant extracts for the light spot of skin-whitening, which is characterized in that raw material components include:
Rice fermentation liquid, 5-15 parts by weight；
European verbena leaf extracting solution, 3-7 parts by weight；
Aloe leaf extract, 5-15 parts by weight；
Radix sophorae extracting solution, 3-7 parts by weight；
Allantoin, 15-25 parts by weight；
The rice fermentation liquid is prepared with the following method:
(A1) rice is taken, after soaking in water, is cooked, shortening rice is obtained；
(A2) aspergillus oryzae is inoculated with into shortening rice described in step (A1), the inoculation quality of the aspergillus oryzae accounts for the shortening rice
The 1-3% of meal quality carries out cultivating 6-9 days later under conditions of temperature is 35-37 DEG C, humidity is 90-95%, be centrifuged,
Filtering is to get the rice fermentation liquid.
2. the plant extracts according to claim 1 for the light spot of skin-whitening, which is characterized in that the european verbena leaf
Extracting solution is prepared with the following method:
(B1) european verbena leaf is taken, through drying, crushes, obtains european verbena leaf fine powder；
(B2) ethyl alcohol is added in the european verbena leaf fine powder in step (B1) for the first time, and in 80-85 DEG C of progress refluxing extraction 1-
2h, centrifugation, filtering, obtains the first filtrate and the first filter residue；Ethyl alcohol is added and at 70-80 DEG C second into first filter residue
It carries out heat preservation and extracts 2-4h, centrifugation, filtering obtain the second filtrate and the second filter residue；First filtrate and the second filtrate are closed
And micro porous filtration is carried out to get the european verbena leaf extracting solution after removing ethyl alcohol is concentrated.
3. the plant extracts according to claim 2 for the light spot of skin-whitening, which is characterized in that in step (B1),
The partial size of the european verbena leaf fine powder is 90-110 mesh；
In step (B2), the volumetric concentration for adding ethyl alcohol twice is 70-90%；The quality and first of the european verbena leaf fine powder
The ratio between the volume of secondary addition ethyl alcohol is 1:15-1:20g/ml, and the quality of first filter residue adds the volume of ethyl alcohol with second
The ratio between be 1:15-1:20g/ml；
The concentration is that water-bath is concentrated, and the temperature of the concentration is 35-45 DEG C, and the filter sizes for carrying out the micro porous filtration are
0.4-0.5μm。
4. the plant extracts according to claim 1 for the light spot of skin-whitening, which is characterized in that the Chinese aloe leaf mentions
Liquid is taken to be prepared with the following method:
(C1) Chinese aloe leaf is taken, smashes and is slurried after peeling, obtains aloe slurries；
(C2) ethyl alcohol is added in the aloe slurries in step (C1), after impregnating 20-40min, centrifugation, filtering obtain solid content；
Distilled water is added for the first time into the solid content, after impregnating 20-40min, heat preservation is carried out at 90-100 DEG C and extracts 1-2h, from
The heart, filtering obtain the first filtrate and the first filter residue；Into first filter residue second be added distilled water and 90-100 DEG C into
1-2h is extracted in row heat preservation, and centrifugation, filtering obtain the second filtrate and the second filter residue；First filtrate is merged with the second filtrate,
Concentration carries out micro porous filtration after removing distilled water to get the aloe leaf extract.
5. the plant extracts according to claim 4 for the light spot of skin-whitening, which is characterized in that in step (C2),
The mass ratio of the ethyl alcohol and the aloe slurries is 1.5:1-2.5:1, and the volumetric concentration of the ethyl alcohol is 70-90%；
The solid content and the mass ratio for adding distilled water for the first time are 1:8-1:12；First filter residue is steamed with second of addition
The mass ratio of distilled water is 1:8-1:12；
The concentration is that water-bath is concentrated, and the temperature of the concentration is 85-95 DEG C, and the filter sizes for carrying out the micro porous filtration are
0.4-0.5μm。
6. the plant extracts according to claim 1 for the light spot of skin-whitening, which is characterized in that the radix sophorae mentions
Liquid is taken to be prepared with the following method:
(D1) radix sophorae is taken, through drying, crushes, obtains radix sophorae fine powder；
(D2) be added distilled water in the radix sophorae fine powder in step (D1) for the first time, after impregnating 20-40min, 90-95 DEG C into
Row refluxing extraction 1-2h, centrifugation, filtering, obtains the first filtrate and the first filter residue；It is added and steams with second to first filter residue
Distilled water simultaneously carries out heat preservation extraction 1-2h at 90-95 DEG C, and centrifugation, filtering obtain the second filtrate and the second filter residue；Described first is filtered
Liquid merges with the second filtrate, and concentration carries out micro porous filtration after removing distilled water to get the radix sophorae extracting solution.
7. the plant extracts according to claim 6 for the light spot of skin-whitening, which is characterized in that in step (D1),
The partial size of the radix sophorae fine powder is 35-45 mesh；
In step (D2), the radix sophorae fine powder and the mass ratio for adding distilled water for the first time are 1:18-1:22；First filter
The mass ratio of slag and second of addition distilled water is 1:18-1:22；
The concentration is that water-bath is concentrated, and the temperature of the concentration is 85-95 DEG C, and the filter sizes for carrying out the micro porous filtration are
0.4-0.5μm。
8. a kind of preparation method of the described in any item plant extracts for the light spot of skin-whitening of claim 1-7, special
Sign is, specific steps are as follows:
The rice fermentation liquid, european verbena leaf extracting solution, aloe leaf extract and radix sophorae extracting solution are taken respectively and are mixed
Afterwards, allantoin is added, is sufficiently mixed uniformly to get the plant extracts.
9. application of any one of the claim 1-7 plant extracts in the skin care item that preparation is used for the light spot of skin-whitening.
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