CN106753892A - A kind of Green antimicrobial cleanser essence and preparation method thereof - Google Patents
A kind of Green antimicrobial cleanser essence and preparation method thereof Download PDFInfo
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- CN106753892A CN106753892A CN201610950384.2A CN201610950384A CN106753892A CN 106753892 A CN106753892 A CN 106753892A CN 201610950384 A CN201610950384 A CN 201610950384A CN 106753892 A CN106753892 A CN 106753892A
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
- C11D1/88—Ampholytes; Electroneutral compounds
- C11D1/94—Mixtures with anionic, cationic or non-ionic compounds
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/382—Vegetable products, e.g. soya meal, wood flour, sawdust
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/48—Medical, disinfecting agents, disinfecting, antibacterial, germicidal or antimicrobial compositions
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
- C11D1/66—Non-ionic compounds
- C11D1/662—Carbohydrates or derivatives
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
- C11D1/88—Ampholytes; Electroneutral compounds
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
- C11D1/88—Ampholytes; Electroneutral compounds
- C11D1/90—Betaines
Abstract
The present invention provides a kind of Green antimicrobial cleanser essence and preparation method thereof, liquid detergent prepared by the present invention is with natural plant composition as primary raw material, the compound prepared by natural plant extracts is used as antiseptic, antibiotic and sterilizing cooperative effect based on shitosan amphoteric polymeric surfactant Yu natural plant extracts, its antibacterial activity is greatly improved, with has a broad antifungal spectrum, antimicrobial efficiency is high the characteristics of, while effective sterilized, dirt can be removed and agriculture is residual, and play a part of taste removal.
Description
Technical field
The present invention relates to a kind of liquid detergent, particularly a kind of Green antimicrobial cleanser essence and preparation method thereof.
Background technology
With the development of science and technology, the living standard of people is improved constantly, and its sanitation and hygiene consciousness also increasingly strengthens.Day
Often in life, people commonly use commercially available liquid detergent cleaning tableware, melon fruits and vegetables and birds, beasts and eggs etc., residual to reach removing dirt and agriculture
Purpose.But, the toxicity of major part liquid detergent is larger at present, and the excitant to skin is strong, it is impossible to biodegradable, can be right when using
Washed article brings chemical residual, and pollutes the environment.Additionally, the qualification rate of presently commercially available liquid detergent is relatively low, using this
A little murders by poisoning of the substandard product to human body and environment are bigger.Therefore, it is very to develop gentle, safe and effective Novel detergent
It is necessary.
It is near that people begin to use antibacterial tableware detergent, not only can remove dirt when using and agriculture is residual during the last ten years, and
And harmful microorganism can be killed, therefore the disinfecting steps of tableware can be reduced.But, in current antibacterial tableware detergent
Based on chloride, there is toxicity in such as chlorine dioxide, triclosan, DCCNa and Trichlorocarbanilide with antiseptic
Greatly, the shortcoming of stability difference；Also have that to use natural plant extracts be Antibacterial Constituents, such as Wedelia trilobata volatilization
Oil and wormwood volatile oil etc., but its antibacterial activity is not enough, it is impossible to meet the actual antibacterial requirement of liquid detergent.
The content of the invention
In view of this, the present invention provides a kind of Green antimicrobial cleanser essence and preparation method thereof, liquid detergent prepared by the present invention
With natural plant composition as primary raw material, the compound prepared by natural plant extracts is used as antiseptic, based on shitosan
The antibiotic and sterilizing cooperative effect of amphoteric polymeric surfactant and natural plant extracts, its antibacterial activity is greatly improved, and is had
There is has a broad antifungal spectrum, the characteristics of antimicrobial efficiency is high, while effective sterilized, dirt can be removed and agriculture is residual, and play taste removal
Effect.
The technical scheme is that：A kind of Green antimicrobial cleanser essence, by following parts by weight into being grouped into：Shitosan
Amphoteric polymeric surfactant 34-52 parts, Dodecyl Polyglucosides 12-23 parts, lauroylamidopropyl betaine 22-34
Part, deionized water 8-14 parts, thyme essential oil 2-5 parts, 13-17 parts of adiantum cpilus-veneris extract, 14-27 parts of tea seed enzymolysis product.
Further, described Green antimicrobial cleanser essence, by following parts by weight into being grouped into：Shitosan both sexes high score
Sub- surfactant 38-46 parts, Dodecyl Polyglucosides 15-19 parts, lauroylamidopropyl betaine 26-31 parts, deionization
Water 9-12 parts, thyme essential oil 3-5 parts, 14-16 parts of adiantum cpilus-veneris extract, 18-23 parts of tea seed enzymolysis product.
Further, the molecular formula of the shitosan amphoteric polymeric surfactant is,
Wherein n=10-22.
Further, the shitosan amphoteric polymeric surfactant is with natural chitosan as raw material, with long-chain fat
Fat aldehyde, 3- chlorine-2-hydroxyls propane sulfonic acid are used as a kind of synthetically prepared water-soluble high score based on shitosan for obtaining of alkylating reagent
Sub- surfactant.
Further, the preparation method of the tea seed enzymolysis product is：The pretreatments such as tea seed is size-reduced, sieving and impurity elimination
Afterwards, with solid-liquid ratio 1: 5（m/V）Add water, soaked overnight, by the way of trypsase and papain mixed enzymolysis, enzymolysis
Condition is：The enzyme concentration of 45 DEG C of temperature, pH 7.5, the .5h of hydrolysis time 2, trypsase and papain is respectively
1500 U/g and 1300U/g；The enzyme that gone out after terminating is digested, 5000r/min is centrifuged 10min at 4 DEG C by enzymolysis liquid, takes supernatant,
Again tea seed enzymolysis product is obtained by micro-filtration.
Further, the preparation method of the adiantum cpilus-veneris extraction product is：By adiantum cpilus-veneris clean dry, then adiantum cpilus-veneris is ground
50 mesh sieves are clayed into power and cross, the adiantum cpilus-veneris powder after sieving adds 70% ethanol, the quality (g) of adiantum cpilus-veneris powder
With the volume (mL) of ethanol than m: V=1: 13；Supersonic oscillations instrument (40 DEG C, 450 W) min of assisted extraction 20；Pig
Hair on the neck grass ethanol flows back through water-bath extract again, and extraction temperature is 65 DEG C, extraction 3 times, every time 1.5 h, suction filtration centrifugation
(5000r/min, 15min), rotary evaporation in vacuo reclaims ethanol after filtering, obtains adiantum cpilus-veneris crude extract；HPD-300 is big
Hole resin soaks 24 h with 95% ethanol, and distilled water is cleaned to after without ethanol flavor and is put into 20 cm × 240 cm macropore trees
The special chromatographic column of fat；Adiantum cpilus-veneris crude extract is with 400 BV/h (BV:The volume of macroreticular resin) the rate of adsorption be adsorbed in
On HPD-300 macroreticular resins, upper column quantity is 600 BV；The resin for the having adsorbed distilled water of 800 BV is with 1000 BV
Impurity on the flow velocity eluted resin of/h, then the crude extract being attached on resin is carried out with 60% ethanol of 1200 BV
Wash-out, obtains adiantum cpilus-veneris and extracts product after wash-out liquid condensing concentration.
A kind of preparation method of Green antimicrobial cleanser essence, including step in detail below：By deionized water add reactor in,
70 DEG C are heated to, lauroylamidopropyl betaine, Dodecyl Polyglucosides, shitosan both sexes high score are sequentially added by weight
After sub- surfactant stirs, temperature is down to 40 DEG C, and the PH values of solution are adjusted into 6.8-7.2, adds adiantum cpilus-veneris to extract
Thing, tea seed enzymolysis product, thyme essence, is stirred until homogeneous, and the feed liquid that will be stirred is de-gassed, and using vacuum outgas, bears
Voltage-controlled system is dispensed, you can obtain Green antimicrobial cleanser essence in 5Mpa to mixed liquor.
The beneficial effects of the present invention are：Modern design, combination is reasonable, and manufacture is easy, it is easy to industrialized production；Using this
Inventing the alternative liquid detergent common in the market of liquid detergent developed is used for family, the meal in the place such as hotel and all kinds of dining rooms
The cleaning of tool, fruit and vegetables, while having the functions, effect is significant such as deodorization and sterilization, removal residues of pesticides；Shell in the composition
The surfactants such as glycan amphoteric polymeric surfactant do not only have excellent washing performance, and possess nontoxic, non-stimulated, biology
The performances such as degraded is rapid, safety, are the comprehensive green surfactants of performance；Thyme essential oil in the present invention, adiantum cpilus-veneris is extracted
Thing, is not chemical industry synthesis product containing abundant bioactive ingredients in tea seed enzymolysis product, can substitute what is used in liquid detergent
Chemical solvent, cleaning performance is good, while the cell membrane and cell of pathogenic bacteria by the Synergistic of each component, can be acted on
Film system, destroys directly and rapidly the energetic supersession and cell that depend on membrane structure integrality and organelle is depended on for existence to thing
The selective permeability of matter, makes nutriment and metabolite normally through cell membrane microorganism cannot be made to lose nutrition and cause
Its growth retardation, reaches the purpose of restraining and sterilizing bacteria；Or by suppressing the respiration of pathogenic bacteria, metabolic activity is obstructed,
Normal dynamic membrane structure can not be maintained, causes the self-dissolving of cell.By the abundant hydroxyl contained in the active component in invention
The groups such as base, easily form hydrogen bond with active site in alcohol, and then cause metabolic system disorderly, and then reach the effect of antibiotic and sterilizing
Really.It is in use non-harmful, with natural plant fragrance, can goes to eliminate the unusual smell, skin care does not stimulate.
Specific embodiment
Technical scheme is clearly and completely described below in conjunction with the embodiment of the present invention, it is clear that retouched
The embodiment stated is only a part of embodiment of the invention, rather than whole embodiments.Based on the embodiment in the present invention, this
The every other embodiment that field those of ordinary skill is obtained under the premise of creative work is not made, belongs to the present invention
The scope of protection.
Embodiment 1
The present embodiment provides a kind of Green antimicrobial cleanser essence, Green antimicrobial cleanser essence, by following parts by weight into being grouped into：Shell
43 parts of glycan amphoteric polymeric surfactant, 17 parts of Dodecyl Polyglucosides, 28 parts of lauroylamidopropyl betaine, go from
11 parts of sub- water, 4 parts of thyme essential oil, 15 parts of adiantum cpilus-veneris extract, 21 parts of tea seed enzymolysis product.
Further, the molecular formula of the shitosan amphoteric polymeric surfactant is,
Wherein n=16.
Further, the shitosan amphoteric polymeric surfactant is with natural chitosan as raw material, with long-chain fat
Fat aldehyde, 3- chlorine-2-hydroxyls propane sulfonic acid are used as a kind of synthetically prepared water-soluble high score based on shitosan for obtaining of alkylating reagent
Sub- surfactant.
Further, the preparation method of the tea seed enzymolysis product is：The pretreatments such as tea seed is size-reduced, sieving and impurity elimination
Afterwards, with solid-liquid ratio 1: 5（m/V）Add water, soaked overnight, by the way of trypsase and papain mixed enzymolysis, enzymolysis
Condition is：The enzyme concentration of 45 DEG C of temperature, pH 7.5, the .5h of hydrolysis time 2, trypsase and papain is respectively
1500 U/g and 1300U/g；The enzyme that gone out after terminating is digested, 5000r/min is centrifuged 10min at 4 DEG C by enzymolysis liquid, takes supernatant,
Again tea seed enzymolysis product is obtained by micro-filtration.
Further, the preparation method of the adiantum cpilus-veneris extraction product is：By adiantum cpilus-veneris clean dry, then adiantum cpilus-veneris is ground
50 mesh sieves are clayed into power and cross, the adiantum cpilus-veneris powder after sieving adds 70% ethanol, the quality (g) of adiantum cpilus-veneris powder
With the volume (mL) of ethanol than m: V=1: 13；Supersonic oscillations instrument (40 DEG C, 450 W) min of assisted extraction 20；Pig
Hair on the neck grass ethanol flows back through water-bath extract again, and extraction temperature is 65 DEG C, extraction 3 times, every time 1.5 h, suction filtration centrifugation
(5000r/min, 15min), rotary evaporation in vacuo reclaims ethanol after filtering, obtains adiantum cpilus-veneris crude extract；HPD-300 is big
Hole resin soaks 24 h with 95% ethanol, and distilled water is cleaned to after without ethanol flavor and is put into 20 cm × 240 cm macropore trees
The special chromatographic column of fat；Adiantum cpilus-veneris crude extract is with 400 BV/h (BV:The volume of macroreticular resin) the rate of adsorption be adsorbed in
On HPD-300 macroreticular resins, upper column quantity is 600 BV；The resin for the having adsorbed distilled water of 800 BV is with 1000 BV
Impurity on the flow velocity eluted resin of/h, then the crude extract being attached on resin is carried out with 60% ethanol of 1200 BV
Wash-out, obtains adiantum cpilus-veneris and extracts product after wash-out liquid condensing concentration.
A kind of preparation method of Green antimicrobial cleanser essence, including step in detail below：By deionized water add reactor in,
70 DEG C are heated to, lauroylamidopropyl betaine, Dodecyl Polyglucosides, shitosan both sexes high score are sequentially added by weight
After sub- surfactant stirs, temperature is down to 40 DEG C, and the PH values of solution are adjusted into 6.8, adds adiantum cpilus-veneris extract, tea
Seed enzymolysis product, thyme essence, are stirred until homogeneous, and the feed liquid that will be stirred is de-gassed, using vacuum outgas, vacuum cavitations
In 5Mpa, mixed liquor is dispensed, you can obtain Green antimicrobial cleanser essence.
Embodiment 2
The present embodiment provides a kind of Green antimicrobial cleanser essence, Green antimicrobial cleanser essence, by following parts by weight into being grouped into：Shell
43 parts of glycan amphoteric polymeric surfactant, 17 parts of Dodecyl Polyglucosides, 28 parts of lauroylamidopropyl betaine, go from
11 parts of sub- water, 3 parts of thyme essential oil, 16 parts of adiantum cpilus-veneris extract, 18 parts of tea seed enzymolysis product.
Further, the molecular formula of the shitosan amphoteric polymeric surfactant is,
Wherein n=18.
Further, the shitosan amphoteric polymeric surfactant is with natural chitosan as raw material, with long-chain fat
Fat aldehyde, 3- chlorine-2-hydroxyls propane sulfonic acid are used as a kind of synthetically prepared water-soluble high score based on shitosan for obtaining of alkylating reagent
Sub- surfactant.
Further, the preparation method of the tea seed enzymolysis product is：The pretreatments such as tea seed is size-reduced, sieving and impurity elimination
Afterwards, with solid-liquid ratio 1: 5（m/V）Add water, soaked overnight, by the way of trypsase and papain mixed enzymolysis, enzymolysis
Condition is：The enzyme concentration of 45 DEG C of temperature, pH 7.5, the .5h of hydrolysis time 2, trypsase and papain is respectively
1500 U/g and 1300U/g；The enzyme that gone out after terminating is digested, 5000r/min is centrifuged 10min at 4 DEG C by enzymolysis liquid, takes supernatant,
Again tea seed enzymolysis product is obtained by micro-filtration.
Further, the preparation method of the adiantum cpilus-veneris extraction product is：By adiantum cpilus-veneris clean dry, then adiantum cpilus-veneris is ground
50 mesh sieves are clayed into power and cross, the adiantum cpilus-veneris powder after sieving adds 70% ethanol, the quality (g) of adiantum cpilus-veneris powder
With the volume (mL) of ethanol than m: V=1: 13；Supersonic oscillations instrument (40 DEG C, 450 W) min of assisted extraction 20；Pig
Hair on the neck grass ethanol flows back through water-bath extract again, and extraction temperature is 65 DEG C, extraction 3 times, every time 1.5 h, suction filtration centrifugation
(5000r/min, 15min), rotary evaporation in vacuo reclaims ethanol after filtering, obtains adiantum cpilus-veneris crude extract；HPD-300 is big
Hole resin soaks 24 h with 95% ethanol, and distilled water is cleaned to after without ethanol flavor and is put into 20 cm × 240 cm macropore trees
The special chromatographic column of fat；Adiantum cpilus-veneris crude extract is with 400 BV/h (BV:The volume of macroreticular resin) the rate of adsorption be adsorbed in
On HPD-300 macroreticular resins, upper column quantity is 600 BV；The resin for the having adsorbed distilled water of 800 BV is with 1000 BV
Impurity on the flow velocity eluted resin of/h, then the crude extract being attached on resin is carried out with 60% ethanol of 1200 BV
Wash-out, obtains adiantum cpilus-veneris and extracts product after wash-out liquid condensing concentration.
A kind of preparation method of Green antimicrobial cleanser essence, including step in detail below：By deionized water add reactor in,
70 DEG C are heated to, lauroylamidopropyl betaine, Dodecyl Polyglucosides, shitosan both sexes high score are sequentially added by weight
After sub- surfactant stirs, temperature is down to 40 DEG C, and the PH values of solution are adjusted into 7.2, adds adiantum cpilus-veneris extract, tea
Seed enzymolysis product, thyme essence, are stirred until homogeneous, and the feed liquid that will be stirred is de-gassed, using vacuum outgas, vacuum cavitations
In 5Mpa, mixed liquor is dispensed, you can obtain Green antimicrobial cleanser essence.
Embodiment 3
The present embodiment provides a kind of Green antimicrobial cleanser essence, Green antimicrobial cleanser essence, by following parts by weight into being grouped into：Shell
41 parts of glycan amphoteric polymeric surfactant, 17 parts of Dodecyl Polyglucosides, 29 parts of lauroylamidopropyl betaine, deionization
11 parts of water, 5 parts of thyme essential oil, 14 parts of adiantum cpilus-veneris extract, 23 parts of tea seed enzymolysis product.
Further, the molecular formula of the shitosan amphoteric polymeric surfactant is,
Wherein n=14.
Further, the shitosan amphoteric polymeric surfactant is with natural chitosan as raw material, with long-chain fat
Fat aldehyde, 3- chlorine-2-hydroxyls propane sulfonic acid are used as a kind of synthetically prepared water-soluble high score based on shitosan for obtaining of alkylating reagent
Sub- surfactant.
Further, the preparation method of the tea seed enzymolysis product is：The pretreatments such as tea seed is size-reduced, sieving and impurity elimination
Afterwards, with solid-liquid ratio 1: 5（m/V）Add water, soaked overnight, by the way of trypsase and papain mixed enzymolysis, enzymolysis
Condition is：The enzyme concentration of 45 DEG C of temperature, pH 7.5, the .5h of hydrolysis time 2, trypsase and papain is respectively
1500 U/g and 1300U/g；The enzyme that gone out after terminating is digested, 5000r/min is centrifuged 10min at 4 DEG C by enzymolysis liquid, takes supernatant,
Again tea seed enzymolysis product is obtained by micro-filtration.
Further, the preparation method of the adiantum cpilus-veneris extraction product is：By adiantum cpilus-veneris clean dry, then adiantum cpilus-veneris is ground
50 mesh sieves are clayed into power and cross, the adiantum cpilus-veneris powder after sieving adds 70% ethanol, the quality (g) of adiantum cpilus-veneris powder
With the volume (mL) of ethanol than m: V=1: 13；Supersonic oscillations instrument (40 DEG C, 450 W) min of assisted extraction 20；Pig
Hair on the neck grass ethanol flows back through water-bath extract again, and extraction temperature is 65 DEG C, extraction 3 times, every time 1.5 h, suction filtration centrifugation
(5000r/min, 15min), rotary evaporation in vacuo reclaims ethanol after filtering, obtains adiantum cpilus-veneris crude extract；HPD-300 is big
Hole resin soaks 24 h with 95% ethanol, and distilled water is cleaned to after without ethanol flavor and is put into 20 cm × 240 cm macropore trees
The special chromatographic column of fat；Adiantum cpilus-veneris crude extract is with 400 BV/h (BV:The volume of macroreticular resin) the rate of adsorption be adsorbed in
On HPD-300 macroreticular resins, upper column quantity is 600 BV；The resin for the having adsorbed distilled water of 800 BV is with 1000 BV
Impurity on the flow velocity eluted resin of/h, then the crude extract being attached on resin is carried out with 60% ethanol of 1200 BV
Wash-out, obtains adiantum cpilus-veneris and extracts product after wash-out liquid condensing concentration.
A kind of preparation method of Green antimicrobial cleanser essence, including step in detail below：By deionized water add reactor in,
70 DEG C are heated to, lauroylamidopropyl betaine, Dodecyl Polyglucosides, shitosan both sexes high score are sequentially added by weight
After sub- surfactant stirs, temperature is down to 40 DEG C, and the PH values of solution are adjusted into 7.0, adds adiantum cpilus-veneris extract, tea
Seed enzymolysis product, thyme essence, are stirred until homogeneous, and the feed liquid that will be stirred is de-gassed, using vacuum outgas, vacuum cavitations
In 5Mpa, mixed liquor is dispensed, you can obtain Green antimicrobial cleanser essence.
Embodiment 4
The present embodiment provides a kind of Green antimicrobial cleanser essence, Green antimicrobial cleanser essence, by following parts by weight into being grouped into：Shell
38 parts of glycan amphoteric polymeric surfactant, 15 parts of Dodecyl Polyglucosides, 26 parts of lauroylamidopropyl betaine, go from
9 parts of sub- water, 4 parts of thyme essential oil, 15 parts of adiantum cpilus-veneris extract, 21 parts of tea seed enzymolysis product.
Further, the molecular formula of the shitosan amphoteric polymeric surfactant is,
Wherein n=20.
Further, the shitosan amphoteric polymeric surfactant is with natural chitosan as raw material, with long-chain fat
Fat aldehyde, 3- chlorine-2-hydroxyls propane sulfonic acid are used as a kind of synthetically prepared water-soluble high score based on shitosan for obtaining of alkylating reagent
Sub- surfactant.
Further, the preparation method of the tea seed enzymolysis product is：The pretreatments such as tea seed is size-reduced, sieving and impurity elimination
Afterwards, with solid-liquid ratio 1: 5（m/V）Add water, soaked overnight, by the way of trypsase and papain mixed enzymolysis, enzymolysis
Condition is：The enzyme concentration of 45 DEG C of temperature, pH 7.5, the .5h of hydrolysis time 2, trypsase and papain is respectively
1500 U/g and 1300U/g；The enzyme that gone out after terminating is digested, 5000r/min is centrifuged 10min at 4 DEG C by enzymolysis liquid, takes supernatant,
Again tea seed enzymolysis product is obtained by micro-filtration.
Further, the preparation method of the adiantum cpilus-veneris extraction product is：By adiantum cpilus-veneris clean dry, then adiantum cpilus-veneris is ground
50 mesh sieves are clayed into power and cross, the adiantum cpilus-veneris powder after sieving adds 70% ethanol, the quality (g) of adiantum cpilus-veneris powder
With the volume (mL) of ethanol than m: V=1: 13；Supersonic oscillations instrument (40 DEG C, 450 W) min of assisted extraction 20；Pig
Hair on the neck grass ethanol flows back through water-bath extract again, and extraction temperature is 65 DEG C, extraction 3 times, every time 1.5 h, suction filtration centrifugation
(5000r/min, 15min), rotary evaporation in vacuo reclaims ethanol after filtering, obtains adiantum cpilus-veneris crude extract；HPD-300 is big
Hole resin soaks 24 h with 95% ethanol, and distilled water is cleaned to after without ethanol flavor and is put into 20 cm × 240 cm macropore trees
The special chromatographic column of fat；Adiantum cpilus-veneris crude extract is with 400 BV/h (BV:The volume of macroreticular resin) the rate of adsorption be adsorbed in
On HPD-300 macroreticular resins, upper column quantity is 600 BV；The resin for the having adsorbed distilled water of 800 BV is with 1000 BV
Impurity on the flow velocity eluted resin of/h, then the crude extract being attached on resin is carried out with 60% ethanol of 1200 BV
Wash-out, obtains adiantum cpilus-veneris and extracts product after wash-out liquid condensing concentration.
A kind of preparation method of Green antimicrobial cleanser essence, including step in detail below：By deionized water add reactor in,
70 DEG C are heated to, lauroylamidopropyl betaine, Dodecyl Polyglucosides, shitosan both sexes high score are sequentially added by weight
After sub- surfactant stirs, temperature is down to 40 DEG C, and the PH values of solution are adjusted into 6.9, adds adiantum cpilus-veneris extract, tea
Seed enzymolysis product, thyme essence, are stirred until homogeneous, and the feed liquid that will be stirred is de-gassed, using vacuum outgas, vacuum cavitations
In 5Mpa, mixed liquor is dispensed, you can obtain Green antimicrobial cleanser essence.
Embodiment 5
The present embodiment provides a kind of Green antimicrobial cleanser essence, Green antimicrobial cleanser essence, by following parts by weight into being grouped into：Shell
46 parts of glycan amphoteric polymeric surfactant, 19 parts of Dodecyl Polyglucosides, 31 parts of lauroylamidopropyl betaine, deionization
12 parts of water, 4 parts of thyme essential oil, 15 parts of adiantum cpilus-veneris extract, 21 parts of tea seed enzymolysis product.
Further, the molecular formula of the shitosan amphoteric polymeric surfactant is,
Wherein n=12.
Further, the shitosan amphoteric polymeric surfactant is with natural chitosan as raw material, with long-chain fat
Fat aldehyde, 3- chlorine-2-hydroxyls propane sulfonic acid are used as a kind of synthetically prepared water-soluble high score based on shitosan for obtaining of alkylating reagent
Sub- surfactant.
Further, the preparation method of the tea seed enzymolysis product is：The pretreatments such as tea seed is size-reduced, sieving and impurity elimination
Afterwards, with solid-liquid ratio 1: 5（m/V）Add water, soaked overnight, by the way of trypsase and papain mixed enzymolysis, enzymolysis
Condition is：The enzyme concentration of 45 DEG C of temperature, pH 7.5, the .5h of hydrolysis time 2, trypsase and papain is respectively
1500 U/g and 1300U/g；The enzyme that gone out after terminating is digested, 5000r/min is centrifuged 10min at 4 DEG C by enzymolysis liquid, takes supernatant,
Again tea seed enzymolysis product is obtained by micro-filtration.
Further, the preparation method of the adiantum cpilus-veneris extraction product is：By adiantum cpilus-veneris clean dry, then adiantum cpilus-veneris is ground
50 mesh sieves are clayed into power and cross, the adiantum cpilus-veneris powder after sieving adds 70% ethanol, the quality (g) of adiantum cpilus-veneris powder
With the volume (mL) of ethanol than m: V=1: 13；Supersonic oscillations instrument (40 DEG C, 450 W) min of assisted extraction 20；Pig
Hair on the neck grass ethanol flows back through water-bath extract again, and extraction temperature is 65 DEG C, extraction 3 times, every time 1.5 h, suction filtration centrifugation
(5000r/min, 15min), rotary evaporation in vacuo reclaims ethanol after filtering, obtains adiantum cpilus-veneris crude extract；HPD-300 is big
Hole resin soaks 24 h with 95% ethanol, and distilled water is cleaned to after without ethanol flavor and is put into 20 cm × 240 cm macropore trees
The special chromatographic column of fat；Adiantum cpilus-veneris crude extract is with 400 BV/h (BV:The volume of macroreticular resin) the rate of adsorption be adsorbed in
On HPD-300 macroreticular resins, upper column quantity is 600 BV；The resin for the having adsorbed distilled water of 800 BV is with 1000 BV
Impurity on the flow velocity eluted resin of/h, then the crude extract being attached on resin is carried out with 60% ethanol of 1200 BV
Wash-out, obtains adiantum cpilus-veneris and extracts product after wash-out liquid condensing concentration.
A kind of preparation method of Green antimicrobial cleanser essence, including step in detail below：By deionized water add reactor in,
70 DEG C are heated to, lauroylamidopropyl betaine, Dodecyl Polyglucosides, shitosan both sexes high score are sequentially added by weight
After sub- surfactant stirs, temperature is down to 40 DEG C, and the PH values of solution are adjusted into 7.1, adds adiantum cpilus-veneris extract, tea
Seed enzymolysis product, thyme essence, are stirred until homogeneous, and the feed liquid that will be stirred is de-gassed, using vacuum outgas, vacuum cavitations
In 5Mpa, mixed liquor is dispensed, you can obtain Green antimicrobial cleanser essence.
Comparative example 1
The present embodiment provides a kind of Green antimicrobial cleanser essence, Green antimicrobial cleanser essence, by following parts by weight into being grouped into：
17 parts of Dodecyl Polyglucosides, 28 parts of lauroylamidopropyl betaine, 11 parts of deionized water, 4 parts of thyme essential oil, adiantum cpilus-veneris
15 parts of extract, 21 parts of tea seed enzymolysis product.
Further, the preparation method of the tea seed enzymolysis product is：The pretreatments such as tea seed is size-reduced, sieving and impurity elimination
Afterwards, with solid-liquid ratio 1: 5（m/V）Add water, soaked overnight, by the way of trypsase and papain mixed enzymolysis, enzymolysis
Condition is：The enzyme concentration of 45 DEG C of temperature, pH 7.5, the .5h of hydrolysis time 2, trypsase and papain is respectively
1500 U/g and 1300U/g；The enzyme that gone out after terminating is digested, 5000r/min is centrifuged 10min at 4 DEG C by enzymolysis liquid, takes supernatant,
Again tea seed enzymolysis product is obtained by micro-filtration.
Further, the preparation method of the adiantum cpilus-veneris extraction product is：By adiantum cpilus-veneris clean dry, then adiantum cpilus-veneris is ground
50 mesh sieves are clayed into power and cross, the adiantum cpilus-veneris powder after sieving adds 70% ethanol, the quality (g) of adiantum cpilus-veneris powder
With the volume (mL) of ethanol than m: V=1: 13；Supersonic oscillations instrument (40 DEG C, 450 W) min of assisted extraction 20；Pig
Hair on the neck grass ethanol flows back through water-bath extract again, and extraction temperature is 65 DEG C, extraction 3 times, every time 1.5 h, suction filtration centrifugation
(5000r/min, 15min), rotary evaporation in vacuo reclaims ethanol after filtering, obtains adiantum cpilus-veneris crude extract；HPD-300 is big
Hole resin soaks 24 h with 95% ethanol, and distilled water is cleaned to after without ethanol flavor and is put into 20 cm × 240 cm macropore trees
The special chromatographic column of fat；Adiantum cpilus-veneris crude extract is with 400 BV/h (BV:The volume of macroreticular resin) the rate of adsorption be adsorbed in
On HPD-300 macroreticular resins, upper column quantity is 600 BV；The resin for the having adsorbed distilled water of 800 BV is with 1000 BV
Impurity on the flow velocity eluted resin of/h, then the crude extract being attached on resin is carried out with 60% ethanol of 1200 BV
Wash-out, obtains adiantum cpilus-veneris and extracts product after wash-out liquid condensing concentration.
A kind of preparation method of Green antimicrobial cleanser essence, including step in detail below：By deionized water add reactor in,
70 DEG C are heated to, lauroylamidopropyl betaine is sequentially added by weight, after Dodecyl Polyglucosides stir, temperature
40 DEG C are down to, the PH values of solution are adjusted to 7.0, add adiantum cpilus-veneris extract, tea seed enzymolysis product, thyme essence are stirred extremely
Uniformly, the feed liquid that will be stirred is de-gassed, and using vacuum outgas, vacuum cavitations are dispensed in 5Mpa to mixed liquor, you can
Obtain Green antimicrobial cleanser essence.
Comparative example 2
The present embodiment provides a kind of Green antimicrobial cleanser essence, Green antimicrobial cleanser essence, by following parts by weight into being grouped into：Shell
43 parts of glycan amphoteric polymeric surfactant, 17 parts of Dodecyl Polyglucosides, 11 parts of deionized water, 4 parts of thyme essential oil,
15 parts of adiantum cpilus-veneris extract, 21 parts of tea seed enzymolysis product.
Further, the molecular formula of the shitosan amphoteric polymeric surfactant is,
Wherein n=16.
Further, the shitosan amphoteric polymeric surfactant is with natural chitosan as raw material, with long-chain fat
Fat aldehyde, 3- chlorine-2-hydroxyls propane sulfonic acid are used as a kind of synthetically prepared water-soluble high score based on shitosan for obtaining of alkylating reagent
Sub- surfactant.
Further, the preparation method of the tea seed enzymolysis product is：The pretreatments such as tea seed is size-reduced, sieving and impurity elimination
Afterwards, with solid-liquid ratio 1: 5（m/V）Add water, soaked overnight, by the way of trypsase and papain mixed enzymolysis, enzymolysis
Condition is：The enzyme concentration of 45 DEG C of temperature, pH 7.5, the .5h of hydrolysis time 2, trypsase and papain is respectively
1500 U/g and 1300U/g；The enzyme that gone out after terminating is digested, 5000r/min is centrifuged 10min at 4 DEG C by enzymolysis liquid, takes supernatant,
Again tea seed enzymolysis product is obtained by micro-filtration.
Further, the preparation method of the adiantum cpilus-veneris extraction product is：By adiantum cpilus-veneris clean dry, then adiantum cpilus-veneris is ground
50 mesh sieves are clayed into power and cross, the adiantum cpilus-veneris powder after sieving adds 70% ethanol, the quality (g) of adiantum cpilus-veneris powder
With the volume (mL) of ethanol than m: V=1: 13；Supersonic oscillations instrument (40 DEG C, 450 W) min of assisted extraction 20；Pig
Hair on the neck grass ethanol flows back through water-bath extract again, and extraction temperature is 65 DEG C, extraction 3 times, every time 1.5 h, suction filtration centrifugation
(5000r/min, 15min), rotary evaporation in vacuo reclaims ethanol after filtering, obtains adiantum cpilus-veneris crude extract；HPD-300 is big
Hole resin soaks 24 h with 95% ethanol, and distilled water is cleaned to after without ethanol flavor and is put into 20 cm × 240 cm macropore trees
The special chromatographic column of fat；Adiantum cpilus-veneris crude extract is with 400 BV/h (BV:The volume of macroreticular resin) the rate of adsorption be adsorbed in
On HPD-300 macroreticular resins, upper column quantity is 600 BV；The resin for the having adsorbed distilled water of 800 BV is with 1000 BV
Impurity on the flow velocity eluted resin of/h, then the crude extract being attached on resin is carried out with 60% ethanol of 1200 BV
Wash-out, obtains adiantum cpilus-veneris and extracts product after wash-out liquid condensing concentration.
A kind of preparation method of Green antimicrobial cleanser essence, including step in detail below：By deionized water add reactor in,
70 DEG C are heated to, Dodecyl Polyglucosides are sequentially added by weight, the stirring of shitosan amphoteric polymeric surfactant is equal
After even, temperature is down to 40 DEG C, and the PH values of solution are adjusted into 7.0, adds adiantum cpilus-veneris extract, and tea seed enzymolysis product, thyme are fragrant
Essence, is stirred until homogeneous, and the feed liquid that will be stirred is de-gassed, and using vacuum outgas, vacuum cavitations are carried out in 5Mpa to mixed liquor
Packing, you can obtain Green antimicrobial cleanser essence.
Comparative example 3
The present embodiment provides a kind of Green antimicrobial cleanser essence, Green antimicrobial cleanser essence, by following parts by weight into being grouped into：Shell
43 parts of glycan amphoteric polymeric surfactant, 17 parts of Dodecyl Polyglucosides, 28 parts of lauroylamidopropyl betaine, go from
11 parts of sub- water, 4 parts of thyme essential oil, 21 parts of tea seed enzymolysis product.
Further, the molecular formula of the shitosan amphoteric polymeric surfactant is,
Wherein n=16.
Further, the shitosan amphoteric polymeric surfactant is with natural chitosan as raw material, with long-chain fat
Fat aldehyde, 3- chlorine-2-hydroxyls propane sulfonic acid are used as a kind of synthetically prepared water-soluble high score based on shitosan for obtaining of alkylating reagent
Sub- surfactant.
Further, the preparation method of the tea seed enzymolysis product is：The pretreatments such as tea seed is size-reduced, sieving and impurity elimination
Afterwards, with solid-liquid ratio 1: 5（m/V）Add water, soaked overnight, by the way of trypsase and papain mixed enzymolysis, enzymolysis
Condition is：The enzyme concentration of 45 DEG C of temperature, pH 7.5, the .5h of hydrolysis time 2, trypsase and papain is respectively
1500 U/g and 1300U/g；The enzyme that gone out after terminating is digested, 5000r/min is centrifuged 10min at 4 DEG C by enzymolysis liquid, takes supernatant,
Again tea seed enzymolysis product is obtained by micro-filtration.
A kind of preparation method of Green antimicrobial cleanser essence, including step in detail below：By deionized water add reactor in,
70 DEG C are heated to, lauroylamidopropyl betaine, Dodecyl Polyglucosides, shitosan both sexes high score are sequentially added by weight
After sub- surfactant stirs, temperature is down to 40 DEG C, and the PH values of solution are adjusted into 7.0, adds tea seed enzymolysis product, hundred
In essence, be stirred until homogeneous, the feed liquid that will be stirred is de-gassed, using vacuum outgas, vacuum cavitations in 5Mpa, to mixing
Liquid is dispensed, you can obtain Green antimicrobial cleanser essence.
Comparative example 4
The present embodiment provides a kind of Green antimicrobial cleanser essence, Green antimicrobial cleanser essence, by following parts by weight into being grouped into：Shell
43 parts of glycan amphoteric polymeric surfactant, 17 parts of Dodecyl Polyglucosides, 28 parts of lauroylamidopropyl betaine, go from
11 parts of sub- water, 4 parts of thyme essential oil, 15 parts of adiantum cpilus-veneris extract.
Further, the molecular formula of the shitosan amphoteric polymeric surfactant is,
Wherein n=16.
Further, the shitosan amphoteric polymeric surfactant is with natural chitosan as raw material, with long-chain fat
Fat aldehyde, 3- chlorine-2-hydroxyls propane sulfonic acid are used as a kind of synthetically prepared water-soluble high score based on shitosan for obtaining of alkylating reagent
Sub- surfactant.
Further, the preparation method of the adiantum cpilus-veneris extraction product is：By adiantum cpilus-veneris clean dry, then adiantum cpilus-veneris is ground
50 mesh sieves are clayed into power and cross, the adiantum cpilus-veneris powder after sieving adds 70% ethanol, the quality (g) of adiantum cpilus-veneris powder
With the volume (mL) of ethanol than m: V=1: 13；Supersonic oscillations instrument (40 DEG C, 450 W) min of assisted extraction 20；Pig
Hair on the neck grass ethanol flows back through water-bath extract again, and extraction temperature is 65 DEG C, extraction 3 times, every time 1.5 h, suction filtration centrifugation
(5000r/min, 15min), rotary evaporation in vacuo reclaims ethanol after filtering, obtains adiantum cpilus-veneris crude extract；HPD-300 is big
Hole resin soaks 24 h with 95% ethanol, and distilled water is cleaned to after without ethanol flavor and is put into 20 cm × 240 cm macropore trees
The special chromatographic column of fat；Adiantum cpilus-veneris crude extract is with 400 BV/h (BV:The volume of macroreticular resin) the rate of adsorption be adsorbed in
On HPD-300 macroreticular resins, upper column quantity is 600 BV；The resin for the having adsorbed distilled water of 800 BV is with 1000 BV
Impurity on the flow velocity eluted resin of/h, then the crude extract being attached on resin is carried out with 60% ethanol of 1200 BV
Wash-out, obtains adiantum cpilus-veneris and extracts product after wash-out liquid condensing concentration.
A kind of preparation method of Green antimicrobial cleanser essence, including step in detail below：By deionized water add reactor in,
70 DEG C are heated to, lauroylamidopropyl betaine, Dodecyl Polyglucosides, shitosan both sexes high score are sequentially added by weight
After sub- surfactant stirs, temperature is down to 40 DEG C, and the PH values of solution are adjusted into 7.0, adds adiantum cpilus-veneris extract, hundred
In essence, be stirred until homogeneous, the feed liquid that will be stirred is de-gassed, using vacuum outgas, vacuum cavitations in 5Mpa, to mixing
Liquid is dispensed, you can obtain Green antimicrobial cleanser essence.
Comparative example 5
Receive the full effect enriching liquid detergent of good Mrs's brand of Li Xinxihua Co., Ltds production from Hebei, bucket limit makes the date be
20180523, as a comparison case 5.
Comparative example 6
From the lemon aloe liquid detergent of labourer's personal style and attitude board of Guangzhou Nanshun Cleaning Products Co., Ltd.'s production, bucket limit makes the date be
20180803A, as a comparison case 6.
Experimental test
1. bactericidal effect test
Using Suspension quantitative bactericidal test, Escherichia coli, Candida albicans, the S. aureus Inoculate after activation are taken first
Into triangle shaking flask, 37 DEG C, the 160 r/h of min shaken cultivations 36.Then the above-mentioned bacteria suspensions of inoculation 1ml are distinguished respectively
2% dilution with the liquid detergent of 9 ml embodiments 1-5 and comparative example 1-6 mixes, 37 DEG C, 160 r/min vibrations
30min, with dilution the method for falling ware measure detergent to Escherichia coli, Candida albicans, staphylococcus aureus killing logarithm value,
Parallel test in triplicate, calculates bactericidal effect such as following table.
2. detergency experiment
Press《Manual dishwashing detergent》The method of the standards of GB 9985-2000 purchase artificial dirt, 250 ppm hard water,
Instrument needed for dirt ceramics plate and foam level method test detergency.Using foam end point method, respectively Example 1-5 with
And comparative example 1-6 liquid detergent 5mL, by 1000mL concentration for the hard water of 250ppm is poured into enamel basin, separately by 1000mL concentration
For the hard water of 250ppm is put into tubualted bottle.In room temperature（25 ℃）Under, the delivery port of tubualted bottle is opened, hard water is rushed in flowing into basin
Spume.After hard water stops flowing out（The s of process about 45）, plate is scrubbed with 102 mm pigs palm fibre paintbrush, first brush clockwise
5 times, another mistake hour hands brush 5 times, after so repeating 1 time, the dirt major part being applied on plate is washed down, finally again will not
Wash lower part to scrub off, until foam is wholly absent, record the number that washes dishes, and be finally converted into deep bid subnumber, it is as a result as follows
Shown in table.
3. security toxicological test.
The main formula of Example 1 carries out toxicological test, foundation respectively《Foodsafety Toxicological Assessment program and side
Method》GB 15193-2003, using maximum tolerance metering experiment method, dosage is set to 10000 mg/kg BW, from 20
Kunming mice, male and female half and half.The h of animal fasting 16, drinking-water is not limited before experiment.The mg/mL of animal 500 is given using administration by gavage
The sample solution of concentration, gavage amount is 0.4 mL/20 g body weight.The poisoning manifestations and death condition of animal are observed after gavage,
Weigh weekly 1 time, observe 14 d, it is as a result as shown in the table.
| Sample ID | Shitosan amphoteric polymeric surfactant | Adiantum cpilus-veneris extracts product | Tea seed enzymolysis product |
| LD50（mg/kg·BW） | 20000 | 30000 | 40000 |
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, and do not carrying on the back
In the case of spirit or essential attributes of the invention, the present invention can be in other specific forms realized.Therefore, no matter from which
From the point of view of a bit, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention will by appended right
Ask and limited rather than described above, it is intended that all changes that will fall in the implication and scope of the equivalency of claim
Include in the present invention.
Moreover, it will be appreciated that although the present specification is described in terms of embodiments, not each implementation method is only wrapped
Containing an independent technical scheme, this narrating mode of specification is only that for clarity, those skilled in the art should
Specification an as entirety, the technical scheme in each embodiment can also be formed into those skilled in the art through appropriately combined
May be appreciated other embodiment.The ins and outs not described in detail in the present invention, can be by any in this area
Prior art is realized.Particularly, all technical characterstics not described in detail can be realized by any prior art in the present invention.
Claims (7)
1. a kind of Green antimicrobial cleanser essence, it is characterised in that by following parts by weight into being grouped into：Shitosan Amphiphatic high polymer
Surfactant 34-52 parts, Dodecyl Polyglucosides 12-23 parts, lauroylamidopropyl betaine 22-34 parts, deionized water
8-14 parts, thyme essential oil 2-5 parts, 13-17 parts of adiantum cpilus-veneris extract, 14-27 parts of tea seed enzymolysis product.
2. Green antimicrobial cleanser essence according to claim 1, it is characterised in that by following parts by weight into being grouped into：
38-46 parts of shitosan amphoteric polymeric surfactant, Dodecyl Polyglucosides 15-19 parts, lauroylamidopropyl betaine
26-31 parts, deionized water 9-12 parts, thyme essential oil 13-15 parts, 4-6 parts of adiantum cpilus-veneris extract, tea seed enzymolysis product 18-23
Part.
3. Green antimicrobial cleanser essence according to claim 1, it is characterised in that live on the shitosan Amphiphatic high polymer surface
The molecular formula of property agent is, wherein n=10-22.
4. Green antimicrobial cleanser essence according to claim 3, it is characterised in that live on the shitosan Amphiphatic high polymer surface
Property agent be with natural chitosan as raw material, using long-chain fat aldehyde, 3- chlorine-2-hydroxyls propane sulfonic acid as alkylating reagent synthesize make
A kind of standby water soluble polymer surfactant based on shitosan for obtaining.
5. Green antimicrobial cleanser essence according to claim 1, it is characterised in that the preparation method of the tea seed enzymolysis product
For：After the pretreatments such as tea seed is size-reduced, sieving and impurity elimination, with solid-liquid ratio 1: 5（m/V）Add water, soaked overnight, using tryptose
The mode of enzyme and papain mixed enzymolysis, enzymatic hydrolysis condition is：45 DEG C of temperature, pH 7.5, the .5h of hydrolysis time 2, pancreas egg
The enzyme concentration of white enzyme and papain is respectively 1500 U/g and 1300U/g；The enzyme that gone out after terminating is digested, by enzymolysis liquid at 4 DEG C
Lower 5000r/min is centrifuged 10min, takes supernatant, then obtain tea seed enzymolysis product by micro-filtration.
6. Green antimicrobial cleanser essence according to claim 1, it is characterised in that the adiantum cpilus-veneris extracts the preparation side of product
Method is：By adiantum cpilus-veneris clean dry, then adiantum cpilus-veneris grind into powder and 50 mesh sieves are crossed, the adiantum cpilus-veneris powder after sieving adds
Enter 70% ethanol, the quality (g) of adiantum cpilus-veneris powder is with the volume (mL) of ethanol than m: V=1: 13；Supersonic oscillations
Instrument (40 DEG C, 450 W) min of assisted extraction 20；Adiantum cpilus-veneris ethanol flows back through water-bath extract again, and extraction temperature is 65
DEG C, extracting 3 times, 1.5 h every time, suction filtration centrifugation (5000r/min, 15min), rotary evaporation in vacuo is returned after filtering
Ethanol is received, adiantum cpilus-veneris crude extract is obtained；HPD-300 macroreticular resins are soaked into 24 h with 95% ethanol, distilled water is cleaned to nothing
20 cm × 240 special the chromatographic columns of cm macroreticular resins are put into after ethanol flavor；Adiantum cpilus-veneris crude extract is with 400 BV/h (BV:
The volume of macroreticular resin) the rate of adsorption be adsorbed on HPD-300 macroreticular resins, upper column quantity be 600 BV；Adsorb
The resin distilled water of 800 BV with the impurity on the flow velocity eluted resin of 1000 BV/h, then with 1200 BV's
60% ethanol is eluted to the crude extract being attached on resin, and obtaining adiantum cpilus-veneris after wash-out liquid condensing concentration extracts product.
7. the preparation method of a kind of Green antimicrobial cleanser essence as described in any one of claim 1- 6, it is characterised in that including
Step in detail below：By in deionized water addition reactor, 70 DEG C are heated to, dodecanamide propyl is sequentially added by weight
Glycine betaine, Dodecyl Polyglucosides, after shitosan amphoteric polymeric surfactant stirs, temperature is down to 40 DEG C, will be molten
The PH values of liquid are adjusted to 6.8-7.2, add adiantum cpilus-veneris extract, and tea seed enzymolysis product, thyme essence are stirred until homogeneous, will stir
The feed liquid mixed is de-gassed, and using vacuum outgas, vacuum cavitations are dispensed in 5Mpa to mixed liquor, you can obtain green
Antibiotic liquid detergent.
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