CN106753851A - A kind of Food-grade detergent and preparation method thereof - Google Patents
A kind of Food-grade detergent and preparation method thereof Download PDFInfo
- Publication number
- CN106753851A CN106753851A CN201610948425.4A CN201610948425A CN106753851A CN 106753851 A CN106753851 A CN 106753851A CN 201610948425 A CN201610948425 A CN 201610948425A CN 106753851 A CN106753851 A CN 106753851A
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- China
- Prior art keywords
- leaf
- magnolia officinalis
- parts
- stonewort
- food
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 239000003599 detergent Substances 0.000 title claims abstract description 56
- 238000002360 preparation method Methods 0.000 title claims abstract description 48
- 239000007788 liquid Substances 0.000 claims abstract description 74
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 42
- 239000000284 extract Substances 0.000 claims abstract description 38
- 230000001954 sterilising effect Effects 0.000 claims abstract description 26
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 25
- 230000000694 effects Effects 0.000 claims abstract description 16
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 105
- 241001673966 Magnolia officinalis Species 0.000 claims description 105
- 241000196240 Characeae Species 0.000 claims description 74
- 239000000047 product Substances 0.000 claims description 45
- 239000000843 powder Substances 0.000 claims description 31
- 238000005119 centrifugation Methods 0.000 claims description 30
- 238000000605 extraction Methods 0.000 claims description 29
- 239000008367 deionised water Substances 0.000 claims description 25
- 229910021641 deionized water Inorganic materials 0.000 claims description 25
- 235000013761 grape skin extract Nutrition 0.000 claims description 24
- JXUZOXPLXJTGIY-UHFFFAOYSA-N 2-hydroxy-4-oxohenicosane-1,2,3-tricarboxylic acid Chemical compound CCCCCCCCCCCCCCCCCC(=O)C(C(O)=O)C(O)(C(O)=O)CC(O)=O JXUZOXPLXJTGIY-UHFFFAOYSA-N 0.000 claims description 20
- 108090000790 Enzymes Proteins 0.000 claims description 20
- 102000004190 Enzymes Human genes 0.000 claims description 20
- 229920002678 cellulose Polymers 0.000 claims description 20
- 239000001913 cellulose Substances 0.000 claims description 20
- 229940088598 enzyme Drugs 0.000 claims description 20
- MVPICKVDHDWCJQ-UHFFFAOYSA-N ethyl 3-pyrrolidin-1-ylpropanoate Chemical compound CCOC(=O)CCN1CCCC1 MVPICKVDHDWCJQ-UHFFFAOYSA-N 0.000 claims description 20
- 229940045902 sodium stearyl fumarate Drugs 0.000 claims description 20
- 239000006228 supernatant Substances 0.000 claims description 20
- 238000003756 stirring Methods 0.000 claims description 17
- 239000011347 resin Substances 0.000 claims description 16
- 229920005989 resin Polymers 0.000 claims description 16
- 239000000287 crude extract Substances 0.000 claims description 12
- 238000007792 addition Methods 0.000 claims description 11
- 108010059892 Cellulase Proteins 0.000 claims description 10
- 241000191996 Pediococcus pentosaceus Species 0.000 claims description 10
- 230000033228 biological regulation Effects 0.000 claims description 10
- 238000009835 boiling Methods 0.000 claims description 10
- 238000001914 filtration Methods 0.000 claims description 10
- 238000004108 freeze drying Methods 0.000 claims description 10
- 230000035484 reaction time Effects 0.000 claims description 10
- 238000002390 rotary evaporation Methods 0.000 claims description 10
- 238000007873 sieving Methods 0.000 claims description 10
- 238000000967 suction filtration Methods 0.000 claims description 10
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Natural products OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 6
- 241000894006 Bacteria Species 0.000 claims description 3
- -1 Stearoyl citric acid Chemical compound 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims 2
- BSFDDBUSIZRAPB-VHEBQXMUSA-N (e)-2-octadecanoylbut-2-enedioic acid Chemical compound CCCCCCCCCCCCCCCCCC(=O)C(\C(O)=O)=C/C(O)=O BSFDDBUSIZRAPB-VHEBQXMUSA-N 0.000 claims 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims 1
- 238000000855 fermentation Methods 0.000 claims 1
- 230000004151 fermentation Effects 0.000 claims 1
- 239000000796 flavoring agent Substances 0.000 claims 1
- 235000019634 flavors Nutrition 0.000 claims 1
- 239000012535 impurity Substances 0.000 claims 1
- 229910052708 sodium Inorganic materials 0.000 claims 1
- 239000011734 sodium Substances 0.000 claims 1
- 238000001179 sorption measurement Methods 0.000 claims 1
- 239000000126 substance Substances 0.000 abstract description 6
- 230000015556 catabolic process Effects 0.000 abstract description 3
- 239000012459 cleaning agent Substances 0.000 abstract description 3
- 238000005202 decontamination Methods 0.000 abstract description 3
- 230000003588 decontaminative effect Effects 0.000 abstract description 3
- 238000006731 degradation reaction Methods 0.000 abstract description 3
- 239000000463 material Substances 0.000 abstract description 3
- 231100000252 nontoxic Toxicity 0.000 abstract description 3
- 230000003000 nontoxic effect Effects 0.000 abstract description 3
- 230000002195 synergetic effect Effects 0.000 abstract description 3
- 244000276331 Citrus maxima Species 0.000 abstract description 2
- 235000001759 Citrus maxima Nutrition 0.000 abstract description 2
- 241000195474 Sargassum Species 0.000 abstract description 2
- 239000003242 anti bacterial agent Substances 0.000 abstract description 2
- 229940088710 antibiotic agent Drugs 0.000 abstract description 2
- 150000001875 compounds Chemical class 0.000 abstract description 2
- 239000000428 dust Substances 0.000 abstract description 2
- 230000007613 environmental effect Effects 0.000 abstract description 2
- 235000013305 food Nutrition 0.000 abstract description 2
- 239000004519 grease Substances 0.000 abstract description 2
- 239000000575 pesticide Substances 0.000 abstract description 2
- 239000002994 raw material Substances 0.000 abstract description 2
- 235000015096 spirit Nutrition 0.000 abstract description 2
- 239000000052 vinegar Substances 0.000 abstract description 2
- 235000021419 vinegar Nutrition 0.000 abstract description 2
- 239000006286 aqueous extract Substances 0.000 abstract 1
- 230000004913 activation Effects 0.000 description 10
- 239000000835 fiber Substances 0.000 description 10
- 239000000243 solution Substances 0.000 description 10
- 230000000052 comparative effect Effects 0.000 description 8
- 238000000034 method Methods 0.000 description 8
- 239000008233 hard water Substances 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 239000006260 foam Substances 0.000 description 4
- 235000005979 Citrus limon Nutrition 0.000 description 3
- 244000131522 Citrus pyriformis Species 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 230000000844 anti-bacterial effect Effects 0.000 description 3
- 244000052616 bacterial pathogen Species 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000003304 gavage Methods 0.000 description 3
- 241000222122 Candida albicans Species 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- 241000191967 Staphylococcus aureus Species 0.000 description 2
- 229940095731 candida albicans Drugs 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 230000002503 metabolic effect Effects 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 230000002110 toxicologic effect Effects 0.000 description 2
- 231100000027 toxicology Toxicity 0.000 description 2
- 241001116389 Aloe Species 0.000 description 1
- 206010015946 Eye irritation Diseases 0.000 description 1
- 206010053759 Growth retardation Diseases 0.000 description 1
- 238000012449 Kunming mouse Methods 0.000 description 1
- 206010040880 Skin irritation Diseases 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 241000219095 Vitis Species 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 235000011399 aloe vera Nutrition 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 238000007872 degassing Methods 0.000 description 1
- 210000003298 dental enamel Anatomy 0.000 description 1
- 238000004851 dishwashing Methods 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 238000004945 emulsification Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 231100000013 eye irritation Toxicity 0.000 description 1
- 231100000001 growth retardation Toxicity 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 230000000452 restraining effect Effects 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 231100000475 skin irritation Toxicity 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 125000003696 stearoyl group Chemical group O=C([*])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 231100001265 toxicological assessment Toxicity 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
- C11D1/66—Non-ionic compounds
- C11D1/825—Mixtures of compounds all of which are non-ionic
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/382—Vegetable products, e.g. soya meal, wood flour, sawdust
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/48—Medical, disinfecting agents, disinfecting, antibacterial, germicidal or antimicrobial compositions
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
- C11D1/66—Non-ionic compounds
- C11D1/667—Neutral esters, e.g. sorbitan esters
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D2111/00—Cleaning compositions characterised by the objects to be cleaned; Cleaning compositions characterised by non-standard cleaning or washing processes
- C11D2111/10—Objects to be cleaned
- C11D2111/14—Hard surfaces
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Organic Chemistry (AREA)
- Cosmetics (AREA)
Abstract
The present invention provides a kind of Food-grade detergent and preparation method thereof, and liquid detergent of the invention can thoroughly remove the harmful substances such as remains of pesticide and dust, greasy dirt, the worm dirt on veterinary antibiotics surface；Raw material of the present invention it is all can food grade materials, without chemical surface activity auxiliary chemicals, compound essence etc., there is no secondary pollution, not only economized on water during use, it is also very environmentally friendly；This product wipe thing when will not scratch vessel, kitchen tools or tableware to being stained with grease have rapid decontamination and water saving the features such as, it is not necessary to rinse repeatedly；With sargassum tunning, shaddock bark extract, vinegar unstrained spirits aqueous extract reaches the effect of sterilization by the synergistic function of each component as main sterilization component；Cleaning agent low cost of the invention, preparation process is simple, easily, easy to use, nontoxic to human body non-stimulated, biological degradation rate is high, non-secondary pollution, environmental protection.
Description
Technical field
The invention belongs to articles for washing technical field, and in particular to a kind of Food-grade detergent and preparation method thereof.
Background technology
Although the detergent powder for using at present has cleaning function, it is not suitable for human body and eats, does not have after human body intake
Any benefit, excessive intake can also cause damage to human body, and the washed kitchen tools of liquid detergent always have liquid detergent residual, if
Do not rinsed repeatedly, residual liquid detergent can be ingested human body, go down for a long time harmful, rinsing repeatedly can cause water again
The wasting of resources.
The content of the invention
In view of this, the present invention provides a kind of Food-grade detergent and preparation method thereof, to solve of the prior art asking
Topic.
The technical scheme is that：A kind of Food-grade detergent, by following parts by weight into being grouped into：Stearoyl lemon
44-62 parts of lemon acid, deionized water 33-47 parts, sodium stearyl fumarate 12-23 parts, 2-5 parts of stonewort tunning, grape
Bark extract 3-7 parts, Leaf of Magnolia officinalis extracts 14-27 parts of product.
Further, described Food-grade detergent, by following parts by weight into being grouped into：Stearoyl citric acid 51-
58 parts, deionized water 37-43 parts, sodium stearyl fumarate 17-21 parts, 3-4 parts of stonewort tunning, grape skin extract 4-
5 parts, Leaf of Magnolia officinalis extracts 18-25 parts of product.
Further, the preparation method of the stonewort tunning is：It is solid-liquid ratio 1: 10 to impose a condition（m/V）, fiber
Plain enzyme Cellulase ACCF-4740 additions 2%, 55 DEG C of temperature, pH 4.5, the h of reaction time 1.3；Cellulose hydrolyzation
After end, boiling water bath is gone out enzyme 15 min, and 9000 r/min centrifugations are collected supernatant and obtain stonewort cellulose hydrolyzation liquid；Stonewort
Enzymolysis liquid goes out after enzyme activity, regulation pH value to 6.2-6.6, high pressure steam sterilization（121 DEG C, 20 min）, then it is inoculated with volume fraction
The Pediococcus pentosaceus liquid of 1% MRS culture mediums activation to stonewort enzymolysis liquid, fermented by 37 DEG C of constant incubators standings.Hair
After ferment terminates, supernatant freeze-drying is collected in high pressure steam sterilization, 9000 r/min centrifugations, obtains stonewort tunning,
Saved backup under the conditions of -20 DEG C.
Further, the preparation method of the Leaf of Magnolia officinalis extraction product is：The Leaf of Magnolia officinalis extracts the preparation method of product
For：Leaf of Magnolia officinalis is cleaned into stripping and slicing to dry, then Leaf of Magnolia officinalis grind into powder and crosses 50 mesh sieves, the Leaf of Magnolia officinalis powder after sieving
70% ethanol is added, the quality (g) of Leaf of Magnolia officinalis powder is with the volume (mL) of ethanol than m: V=1: 13；Ultrasonic wave shakes
Swing instrument (40 DEG C, 450 W) min of assisted extraction 20；Leaf of Magnolia officinalis ethanol flows back through water-bath extract again, and extraction temperature is 65
DEG C, extracting 3 times, 1.5 h every time, suction filtration centrifugation (5000r/min, 15min), rotary evaporation in vacuo is returned after filtering
Ethanol is received, Leaf of Magnolia officinalis crude extract is obtained；HPD-300 macroreticular resins are soaked 24 with 95% ethanol
A kind of preparation method of Food-grade detergent, it is characterised in that including step in detail below：Deionized water is added and is reacted
In kettle, 70 DEG C are heated to, sodium stearyl fumarate is sequentially added by weight, after stearoyl citric acid stirs, temperature
40 DEG C are down to, the pH values of solution are adjusted to 6.9, add stonewort tunning, grape skin extract, Leaf of Magnolia officinalis to extract product and stir
Mix to uniform, the feed liquid that will be stirred is de-gassed, and using vacuum outgas, vacuum cavitations are dispensed in 5Mpa to mixed liquor,
Can obtain Food-grade detergent.
The liquid detergent that the present invention is compounded using stearoyl citric acid and sodium stearyl fumarate, with good hydrophilic
Property and lipophile, enhance the mildness of formula system, with good decontamination, emulsification, wetting and foam performance, to skin and
Eye irritation is small, the stabilization in hard water.
The Food-grade detergent that the present invention is provided is with most liquid detergent differences on the market, of the invention to wash
Clean essence can thoroughly remove the harmful substances such as remains of pesticide and dust, greasy dirt, the worm dirt on veterinary antibiotics surface；Raw material of the present invention
It is all can food grade materials, without chemical surface activity auxiliary chemicals, compound essence etc., there is no secondary pollution, use
During not only economize on water, it is also very environmentally friendly；Will not scratch vessel, kitchen tools or the tableware tool to being stained with grease during this product wiping thing
There is the features such as rapid decontamination and water saving, it is not necessary to rinse repeatedly；With sargassum tunning, shaddock bark extract, vinegar unstrained spirits water extraction
Liquid is taken as main sterilization component, the effect of sterilization is reached by the synergistic function of each component；Cleaning agent of the invention
Low cost, preparation process is simple, easily, easy to use, nontoxic to human body non-stimulated, biological degradation rate is high, non-secondary pollution, green
Colour circle is protected.
The present invention is extracted product as main sterilization component, is led to stonewort tunning, grape skin extract, Leaf of Magnolia officinalis
The synergistic function of each component is crossed, pathogenic bacteria are mainly acted in terms of two：1. the cell membrane and cell of pathogenic bacteria are acted on
Film system, destroys directly and rapidly the energetic supersession and cell that depend on membrane structure integrality and organelle is depended on for existence to thing
The selective permeability of matter, makes nutriment and metabolite normally through cell membrane microorganism cannot be made to lose nutrition and cause
Its growth retardation, reaches the purpose of restraining and sterilizing bacteria；2., by suppressing the respiration of pathogenic bacteria, metabolic activity is obstructed, it is impossible to
Normal dynamic membrane structure is maintained, causes the self-dissolving of cell.By abundant hydroxyl for containing in the active component in invention etc.
Group, easily forms hydrogen bond with active site in alcohol, and then causes metabolic system disorderly, and then reaches the effect of sterilization；This hair
Bright cleaning agent low cost, preparation process is simple, easily, easy to use, nontoxic to human body non-stimulated, biological degradation rate is high, nothing
Secondary pollution, environmental protection.
Specific embodiment
Technical scheme is clearly and completely described below in conjunction with the embodiment of the present invention, it is clear that retouched
The embodiment stated is only a part of embodiment of the invention, rather than whole embodiments.Based on the embodiment in the present invention, this
The every other embodiment that field those of ordinary skill is obtained under the premise of creative work is not made, belongs to the present invention
The scope of protection.
Embodiment 1
A kind of Food-grade detergent, by following parts by weight into being grouped into：54 parts of stearoyl citric acid, 39 parts of deionized water,
19 parts of sodium stearyl fumarate, 3 parts of stonewort tunning, 5 parts of grape skin extract, Leaf of Magnolia officinalis extracts 21 parts of product.
Further, the preparation method of the stonewort tunning is：It is solid-liquid ratio 1: 10 to impose a condition（m/V）, fiber
Plain enzyme Cellulase ACCF-4740 additions 2%, 55 DEG C of temperature, pH 4.5, the h of reaction time 1.3；Cellulose hydrolyzation
After end, boiling water bath is gone out enzyme 15 min, and 9000 r/min centrifugations are collected supernatant and obtain stonewort cellulose hydrolyzation liquid；Stonewort
Enzymolysis liquid goes out after enzyme activity, regulation pH value to 6.2-6.6, high pressure steam sterilization（121 DEG C, 20 min）, then it is inoculated with volume fraction
The Pediococcus pentosaceus liquid of 1% MRS culture mediums activation to stonewort enzymolysis liquid, fermented by 37 DEG C of constant incubators standings.Hair
After ferment terminates, supernatant freeze-drying is collected in high pressure steam sterilization, 9000 r/min centrifugations, obtains stonewort tunning,
Saved backup under the conditions of -20 DEG C.
Further, the preparation method of the Leaf of Magnolia officinalis extraction product is：The Leaf of Magnolia officinalis extracts the preparation method of product
For：Leaf of Magnolia officinalis is cleaned into stripping and slicing to dry, then Leaf of Magnolia officinalis grind into powder and crosses 50 mesh sieves, the Leaf of Magnolia officinalis powder after sieving
70% ethanol is added, the quality (g) of Leaf of Magnolia officinalis powder is with the volume (mL) of ethanol than m: V=1: 13；Ultrasonic wave shakes
Swing instrument (40 DEG C, 450 W) min of assisted extraction 20；Leaf of Magnolia officinalis ethanol flows back through water-bath extract again, and extraction temperature is 65
DEG C, extracting 3 times, 1.5 h every time, suction filtration centrifugation (5000r/min, 15min), rotary evaporation in vacuo is returned after filtering
Ethanol is received, Leaf of Magnolia officinalis crude extract is obtained；HPD-300 macroreticular resins are soaked 24 with 95% ethanol
A kind of preparation method of Food-grade detergent, it is characterised in that including step in detail below：Deionized water is added and is reacted
In kettle, 70 DEG C are heated to, sodium stearyl fumarate is sequentially added by weight, after stearoyl citric acid stirs, temperature
40 DEG C are down to, the pH values of solution are adjusted to 6.9, add stonewort tunning, grape skin extract, Leaf of Magnolia officinalis to extract product and stir
Mix to uniform, the feed liquid that will be stirred is de-gassed, and using vacuum outgas, vacuum cavitations are dispensed in 5Mpa to mixed liquor,
Can obtain Food-grade detergent.
Embodiment 2
A kind of Food-grade detergent, by following parts by weight into being grouped into：53 parts of stearoyl citric acid, deionized water 38
Part, 18 parts of sodium stearyl fumarate, 4 parts of stonewort tunning, 4 parts of grape skin extract, Leaf of Magnolia officinalis extracts 18 parts of product.
Further, the preparation method of the stonewort tunning is：It is solid-liquid ratio 1: 10 to impose a condition（m/V）, fiber
Plain enzyme Cellulase ACCF-4740 additions 2%, 55 DEG C of temperature, pH 4.5, the h of reaction time 1.3；Cellulose hydrolyzation
After end, boiling water bath is gone out enzyme 15 min, and 9000 r/min centrifugations are collected supernatant and obtain stonewort cellulose hydrolyzation liquid；Stonewort
Enzymolysis liquid goes out after enzyme activity, regulation pH value to 6.2-6.6, high pressure steam sterilization（121 DEG C, 20 min）, then it is inoculated with volume fraction
The Pediococcus pentosaceus liquid of 1% MRS culture mediums activation to stonewort enzymolysis liquid, fermented by 37 DEG C of constant incubators standings.Hair
After ferment terminates, supernatant freeze-drying is collected in high pressure steam sterilization, 9000 r/min centrifugations, obtains stonewort tunning,
Saved backup under the conditions of -20 DEG C.
Further, the preparation method of the Leaf of Magnolia officinalis extraction product is：The Leaf of Magnolia officinalis extracts the preparation method of product
For：Leaf of Magnolia officinalis is cleaned into stripping and slicing to dry, then Leaf of Magnolia officinalis grind into powder and crosses 50 mesh sieves, the Leaf of Magnolia officinalis powder after sieving
70% ethanol is added, the quality (g) of Leaf of Magnolia officinalis powder is with the volume (mL) of ethanol than m: V=1: 13；Ultrasonic wave shakes
Swing instrument (40 DEG C, 450 W) min of assisted extraction 20；Leaf of Magnolia officinalis ethanol flows back through water-bath extract again, and extraction temperature is 65
DEG C, extracting 3 times, 1.5 h every time, suction filtration centrifugation (5000r/min, 15min), rotary evaporation in vacuo is returned after filtering
Ethanol is received, Leaf of Magnolia officinalis crude extract is obtained；HPD-300 macroreticular resins are soaked 24 with 95% ethanol
A kind of preparation method of Food-grade detergent, it is characterised in that including step in detail below：Deionized water is added and is reacted
In kettle, 70 DEG C are heated to, sodium stearyl fumarate is sequentially added by weight, after stearoyl citric acid stirs, temperature
40 DEG C are down to, the pH values of solution are adjusted to 6.9, add stonewort tunning, grape skin extract, Leaf of Magnolia officinalis to extract product and stir
Mix to uniform, the feed liquid that will be stirred is de-gassed, and using vacuum outgas, vacuum cavitations are dispensed in 5Mpa to mixed liquor,
Can obtain Food-grade detergent.
Embodiment 3
A kind of Food-grade detergent, by following parts by weight into being grouped into：55 parts of stearoyl citric acid, 40 parts of deionized water,
29 parts of sodium stearyl fumarate, 4 parts of stonewort tunning, 5 parts of grape skin extract, Leaf of Magnolia officinalis extracts 25 parts of product.
Further, the preparation method of the stonewort tunning is：It is solid-liquid ratio 1: 10 to impose a condition（m/V）, fiber
Plain enzyme Cellulase ACCF-4740 additions 2%, 55 DEG C of temperature, pH 4.5, the h of reaction time 1.3；Cellulose hydrolyzation
After end, boiling water bath is gone out enzyme 15 min, and 9000 r/min centrifugations are collected supernatant and obtain stonewort cellulose hydrolyzation liquid；Stonewort
Enzymolysis liquid goes out after enzyme activity, regulation pH value to 6.2-6.6, high pressure steam sterilization（121 DEG C, 20 min）, then it is inoculated with volume fraction
The Pediococcus pentosaceus liquid of 1% MRS culture mediums activation to stonewort enzymolysis liquid, fermented by 37 DEG C of constant incubators standings.Hair
After ferment terminates, supernatant freeze-drying is collected in high pressure steam sterilization, 9000 r/min centrifugations, obtains stonewort tunning,
Saved backup under the conditions of -20 DEG C.
Further, the preparation method of the Leaf of Magnolia officinalis extraction product is：The Leaf of Magnolia officinalis extracts the preparation method of product
For：Leaf of Magnolia officinalis is cleaned into stripping and slicing to dry, then Leaf of Magnolia officinalis grind into powder and crosses 50 mesh sieves, the Leaf of Magnolia officinalis powder after sieving
70% ethanol is added, the quality (g) of Leaf of Magnolia officinalis powder is with the volume (mL) of ethanol than m: V=1: 13；Ultrasonic wave shakes
Swing instrument (40 DEG C, 450 W) min of assisted extraction 20；Leaf of Magnolia officinalis ethanol flows back through water-bath extract again, and extraction temperature is 65
DEG C, extracting 3 times, 1.5 h every time, suction filtration centrifugation (5000r/min, 15min), rotary evaporation in vacuo is returned after filtering
Ethanol is received, Leaf of Magnolia officinalis crude extract is obtained；HPD-300 macroreticular resins are soaked 24 with 95% ethanol
A kind of preparation method of Food-grade detergent, it is characterised in that including step in detail below：Deionized water is added and is reacted
In kettle, 70 DEG C are heated to, sodium stearyl fumarate is sequentially added by weight, after stearoyl citric acid stirs, temperature
40 DEG C are down to, the pH values of solution are adjusted to 6.9, add stonewort tunning, grape skin extract, Leaf of Magnolia officinalis to extract product and stir
Mix to uniform, the feed liquid that will be stirred is de-gassed, and using vacuum outgas, vacuum cavitations are dispensed in 5Mpa to mixed liquor,
Can obtain Food-grade detergent.
Embodiment 4
A kind of Food-grade detergent, by following parts by weight into being grouped into：51 parts of stearoyl citric acid, deionized water 37
Part, 17 parts of sodium stearyl fumarate, 3 parts of stonewort tunning, 4 parts of grape skin extract, Leaf of Magnolia officinalis extracts 20 parts of product.
Further, the preparation method of the stonewort tunning is：It is solid-liquid ratio 1: 10 to impose a condition（m/V）, fiber
Plain enzyme Cellulase ACCF-4740 additions 2%, 55 DEG C of temperature, pH 4.5, the h of reaction time 1.3；Cellulose hydrolyzation
After end, boiling water bath is gone out enzyme 15 min, and 9000 r/min centrifugations are collected supernatant and obtain stonewort cellulose hydrolyzation liquid；Stonewort
Enzymolysis liquid goes out after enzyme activity, regulation pH value to 6.2-6.6, high pressure steam sterilization（121 DEG C, 20 min）, then it is inoculated with volume fraction
The Pediococcus pentosaceus liquid of 1% MRS culture mediums activation to stonewort enzymolysis liquid, fermented by 37 DEG C of constant incubators standings.Hair
After ferment terminates, supernatant freeze-drying is collected in high pressure steam sterilization, 9000 r/min centrifugations, obtains stonewort tunning,
Saved backup under the conditions of -20 DEG C.
Further, the preparation method of the Leaf of Magnolia officinalis extraction product is：The Leaf of Magnolia officinalis extracts the preparation method of product
For：Leaf of Magnolia officinalis is cleaned into stripping and slicing to dry, then Leaf of Magnolia officinalis grind into powder and crosses 50 mesh sieves, the Leaf of Magnolia officinalis powder after sieving
70% ethanol is added, the quality (g) of Leaf of Magnolia officinalis powder is with the volume (mL) of ethanol than m: V=1: 13；Ultrasonic wave shakes
Swing instrument (40 DEG C, 450 W) min of assisted extraction 20；Leaf of Magnolia officinalis ethanol flows back through water-bath extract again, and extraction temperature is 65
DEG C, extracting 3 times, 1.5 h every time, suction filtration centrifugation (5000r/min, 15min), rotary evaporation in vacuo is returned after filtering
Ethanol is received, Leaf of Magnolia officinalis crude extract is obtained；HPD-300 macroreticular resins are soaked 24 with 95% ethanol
A kind of preparation method of Food-grade detergent, it is characterised in that including step in detail below：Deionized water is added and is reacted
In kettle, 70 DEG C are heated to, sodium stearyl fumarate is sequentially added by weight, after stearoyl citric acid stirs, temperature
40 DEG C are down to, the pH values of solution are adjusted to 6.9, add stonewort tunning, grape skin extract, Leaf of Magnolia officinalis to extract product and stir
Mix to uniform, the feed liquid that will be stirred is de-gassed, and using vacuum outgas, vacuum cavitations are dispensed in 5Mpa to mixed liquor,
Can obtain Food-grade detergent.
Embodiment 5
A kind of Food-grade detergent, by following parts by weight into being grouped into：58 parts of stearoyl citric acid, 43 parts of deionized water,
21 parts of sodium stearyl fumarate, 4 parts of stonewort tunning, 5 parts of grape skin extract, Leaf of Magnolia officinalis extracts 23 parts of product.
Further, the preparation method of the stonewort tunning is：It is solid-liquid ratio 1: 10 to impose a condition（m/V）, fiber
Plain enzyme Cellulase ACCF-4740 additions 2%, 55 DEG C of temperature, pH 4.5, the h of reaction time 1.3；Cellulose hydrolyzation
After end, boiling water bath is gone out enzyme 15 min, and 9000 r/min centrifugations are collected supernatant and obtain stonewort cellulose hydrolyzation liquid；Stonewort
Enzymolysis liquid goes out after enzyme activity, regulation pH value to 6.2-6.6, high pressure steam sterilization（121 DEG C, 20 min）, then it is inoculated with volume fraction
The Pediococcus pentosaceus liquid of 1% MRS culture mediums activation to stonewort enzymolysis liquid, fermented by 37 DEG C of constant incubators standings.Hair
After ferment terminates, supernatant freeze-drying is collected in high pressure steam sterilization, 9000 r/min centrifugations, obtains stonewort tunning,
Saved backup under the conditions of -20 DEG C.
Further, the preparation method of the Leaf of Magnolia officinalis extraction product is：The Leaf of Magnolia officinalis extracts the preparation method of product
For：Leaf of Magnolia officinalis is cleaned into stripping and slicing to dry, then Leaf of Magnolia officinalis grind into powder and crosses 50 mesh sieves, the Leaf of Magnolia officinalis powder after sieving
70% ethanol is added, the quality (g) of Leaf of Magnolia officinalis powder is with the volume (mL) of ethanol than m: V=1: 13；Ultrasonic wave shakes
Swing instrument (40 DEG C, 450 W) min of assisted extraction 20；Leaf of Magnolia officinalis ethanol flows back through water-bath extract again, and extraction temperature is 65
DEG C, extracting 3 times, 1.5 h every time, suction filtration centrifugation (5000r/min, 15min), rotary evaporation in vacuo is returned after filtering
Ethanol is received, Leaf of Magnolia officinalis crude extract is obtained；HPD-300 macroreticular resins are soaked 24 with 95% ethanol
A kind of preparation method of Food-grade detergent, it is characterised in that including step in detail below：Deionized water is added and is reacted
In kettle, 70 DEG C are heated to, sodium stearyl fumarate is sequentially added by weight, after stearoyl citric acid stirs, temperature
40 DEG C are down to, the pH values of solution are adjusted to 6.9, add stonewort tunning, grape skin extract, Leaf of Magnolia officinalis to extract product and stir
Mix to uniform, the feed liquid that will be stirred is de-gassed, and using vacuum outgas, vacuum cavitations are dispensed in 5Mpa to mixed liquor,
Can obtain Food-grade detergent.
Comparative example 1
A kind of Food-grade detergent, by following parts by weight into being grouped into：39 parts of deionized water, sodium stearyl fumarate 19
Part, 3 parts of stonewort tunning, 5 parts of grape skin extract, Leaf of Magnolia officinalis extracts 21 parts of product.
Further, the preparation method of the stonewort tunning is：It is solid-liquid ratio 1: 10 to impose a condition（m/V）, fiber
Plain enzyme Cellulase ACCF-4740 additions 2%, 55 DEG C of temperature, pH 4.5, the h of reaction time 1.3；Cellulose hydrolyzation
After end, boiling water bath is gone out enzyme 15 min, and 9000 r/min centrifugations are collected supernatant and obtain stonewort cellulose hydrolyzation liquid；Stonewort
Enzymolysis liquid goes out after enzyme activity, regulation pH value to 6.2-6.6, high pressure steam sterilization（121 DEG C, 20 min）, then it is inoculated with volume fraction
The Pediococcus pentosaceus liquid of 1% MRS culture mediums activation to stonewort enzymolysis liquid, fermented by 37 DEG C of constant incubators standings.Hair
After ferment terminates, supernatant freeze-drying is collected in high pressure steam sterilization, 9000 r/min centrifugations, obtains stonewort tunning,
Saved backup under the conditions of -20 DEG C.
Further, the preparation method of the Leaf of Magnolia officinalis extraction product is：The Leaf of Magnolia officinalis extracts the preparation method of product
For：Leaf of Magnolia officinalis is cleaned into stripping and slicing to dry, then Leaf of Magnolia officinalis grind into powder and crosses 50 mesh sieves, the Leaf of Magnolia officinalis powder after sieving
70% ethanol is added, the quality (g) of Leaf of Magnolia officinalis powder is with the volume (mL) of ethanol than m: V=1: 13；Ultrasonic wave shakes
Swing instrument (40 DEG C, 450 W) min of assisted extraction 20；Leaf of Magnolia officinalis ethanol flows back through water-bath extract again, and extraction temperature is 65
DEG C, extracting 3 times, 1.5 h every time, suction filtration centrifugation (5000r/min, 15min), rotary evaporation in vacuo is returned after filtering
Ethanol is received, Leaf of Magnolia officinalis crude extract is obtained；HPD-300 macroreticular resins are soaked 24 with 95% ethanol
A kind of preparation method of Food-grade detergent, it is characterised in that including step in detail below：Deionized water is added and is reacted
In kettle, 70 DEG C are heated to, after adding deionized water to stir by weight, temperature is down to 40 DEG C, and the pH values of solution are adjusted
To 6.9, stonewort tunning, grape skin extract, Leaf of Magnolia officinalis is added to extract product and be stirred until homogeneous, the feed liquid that will be stirred is entered
Row degassing, using vacuum outgas, vacuum cavitations are dispensed in 5Mpa to mixed liquor, you can obtain Food-grade detergent.
Comparative example 2
A kind of Food-grade detergent, by following parts by weight into being grouped into：54 parts of stearoyl citric acid, 39 parts of deionized water,
3 parts of stonewort tunning, 5 parts of grape skin extract, Leaf of Magnolia officinalis extracts 21 parts of product.
Further, the preparation method of the stonewort tunning is：It is solid-liquid ratio 1: 10 to impose a condition（m/V）, fiber
Plain enzyme Cellulase ACCF-4740 additions 2%, 55 DEG C of temperature, pH 4.5, the h of reaction time 1.3；Cellulose hydrolyzation
After end, boiling water bath is gone out enzyme 15 min, and 9000 r/min centrifugations are collected supernatant and obtain stonewort cellulose hydrolyzation liquid；Stonewort
Enzymolysis liquid goes out after enzyme activity, regulation pH value to 6.2-6.6, high pressure steam sterilization（121 DEG C, 20 min）, then it is inoculated with volume fraction
The Pediococcus pentosaceus liquid of 1% MRS culture mediums activation to stonewort enzymolysis liquid, fermented by 37 DEG C of constant incubators standings.Hair
After ferment terminates, supernatant freeze-drying is collected in high pressure steam sterilization, 9000 r/min centrifugations, obtains stonewort tunning,
Saved backup under the conditions of -20 DEG C.
Further, the preparation method of the Leaf of Magnolia officinalis extraction product is：The Leaf of Magnolia officinalis extracts the preparation method of product
For：Leaf of Magnolia officinalis is cleaned into stripping and slicing to dry, then Leaf of Magnolia officinalis grind into powder and crosses 50 mesh sieves, the Leaf of Magnolia officinalis powder after sieving
70% ethanol is added, the quality (g) of Leaf of Magnolia officinalis powder is with the volume (mL) of ethanol than m: V=1: 13；Ultrasonic wave shakes
Swing instrument (40 DEG C, 450 W) min of assisted extraction 20；Leaf of Magnolia officinalis ethanol flows back through water-bath extract again, and extraction temperature is 65
DEG C, extracting 3 times, 1.5 h every time, suction filtration centrifugation (5000r/min, 15min), rotary evaporation in vacuo is returned after filtering
Ethanol is received, Leaf of Magnolia officinalis crude extract is obtained；HPD-300 macroreticular resins are soaked 24 with 95% ethanol
A kind of preparation method of Food-grade detergent, it is characterised in that including step in detail below：Deionized water is added and is reacted
In kettle, 70 DEG C are heated to, after adding stearoyl citric acid to stir by weight, temperature is down to 40 DEG C, by the pH of solution
Value is adjusted to 6.9, adds stonewort tunning, grape skin extract, Leaf of Magnolia officinalis to extract product and be stirred until homogeneous, the material that will be stirred
Liquid is de-gassed, and using vacuum outgas, vacuum cavitations are dispensed in 5Mpa to mixed liquor, you can obtain Food-grade detergent.
Comparative example 3
A kind of Food-grade detergent, by following parts by weight into being grouped into：54 parts of stearoyl citric acid, 39 parts of deionized water,
19 parts of sodium stearyl fumarate, 3 parts of stonewort tunning, 5 parts of grape skin extract.
Further, the preparation method of the stonewort tunning is：It is solid-liquid ratio 1: 10 to impose a condition（m/V）, fiber
Plain enzyme Cellulase ACCF-4740 additions 2%, 55 DEG C of temperature, pH 4.5, the h of reaction time 1.3；Cellulose hydrolyzation
After end, boiling water bath is gone out enzyme 15 min, and 9000 r/min centrifugations are collected supernatant and obtain stonewort cellulose hydrolyzation liquid；Stonewort
Enzymolysis liquid goes out after enzyme activity, regulation pH value to 6.2-6.6, high pressure steam sterilization（121 DEG C, 20 min）, then it is inoculated with volume fraction
The Pediococcus pentosaceus liquid of 1% MRS culture mediums activation to stonewort enzymolysis liquid, fermented by 37 DEG C of constant incubators standings.Hair
After ferment terminates, supernatant freeze-drying is collected in high pressure steam sterilization, 9000 r/min centrifugations, obtains stonewort tunning,
Saved backup under the conditions of -20 DEG C.
A kind of preparation method of Food-grade detergent, it is characterised in that including step in detail below：Deionized water is added
In reactor, 70 DEG C are heated to, sodium stearyl fumarate is sequentially added by weight, after stearoyl citric acid stirs,
Temperature is down to 40 DEG C, and the pH values of solution are adjusted into 6.9, adds stonewort tunning, grape skin extract to be stirred until homogeneous, will
The feed liquid being stirred is de-gassed, and using vacuum outgas, vacuum cavitations are dispensed in 5Mpa to mixed liquor, you can eaten
Grade liquid detergent.
Comparative example 4
A kind of Food-grade detergent, by following parts by weight into being grouped into：54 parts of stearoyl citric acid, 39 parts of deionized water,
19 parts of sodium stearyl fumarate, 5 parts of grape skin extract, Leaf of Magnolia officinalis extracts 21 parts of product.
Further, the preparation method of the Leaf of Magnolia officinalis extraction product is：The Leaf of Magnolia officinalis extracts the preparation method of product
For：Leaf of Magnolia officinalis is cleaned into stripping and slicing to dry, then Leaf of Magnolia officinalis grind into powder and crosses 50 mesh sieves, the Leaf of Magnolia officinalis powder after sieving
70% ethanol is added, the quality (g) of Leaf of Magnolia officinalis powder is with the volume (mL) of ethanol than m: V=1: 13；Ultrasonic wave shakes
Swing instrument (40 DEG C, 450 W) min of assisted extraction 20；Leaf of Magnolia officinalis ethanol flows back through water-bath extract again, and extraction temperature is 65
DEG C, extracting 3 times, 1.5 h every time, suction filtration centrifugation (5000r/min, 15min), rotary evaporation in vacuo is returned after filtering
Ethanol is received, Leaf of Magnolia officinalis crude extract is obtained；HPD-300 macroreticular resins are soaked 24 with 95% ethanol
A kind of preparation method of Food-grade detergent, it is characterised in that including step in detail below：Deionized water is added and is reacted
In kettle, 70 DEG C are heated to, after adding stearoyl citric acid to stir by weight, temperature is down to 40 DEG C, by the pH of solution
Value is adjusted to 6.9, adds grape skin extract, Leaf of Magnolia officinalis to extract product and be stirred until homogeneous, and the feed liquid that will be stirred is de-gassed, and adopts
With vacuum outgas, vacuum cavitations are dispensed in 5Mpa to mixed liquor, you can obtain Food-grade detergent.
Comparative example 5
Receive the full effect enriching liquid detergent of good Mrs's brand of Li Xinxihua Co., Ltds production from Hebei, bucket limit makes the date be
20180523, as a comparison case 5.
Comparative example 6
From the lemon aloe liquid detergent of labourer's personal style and attitude board of Guangzhou Nanshun Cleaning Products Co., Ltd.'s production, bucket limit makes the date be
20180803A, as a comparison case 6.
Experimental test
1. bactericidal effect test
Using Suspension quantitative bactericidal test, the Escherichia coli after activation, staphylococcus aureus, Candida albicans inoculation are taken first
Into triangle shaking flask, 37 DEG C, the 160 r/h of min shaken cultivations 36.Then the above-mentioned bacteria suspensions of inoculation 1ml are distinguished respectively
2% dilution with the liquid detergent of 9 ml embodiments 1-5 and comparative example 1-6 mixes, 37 DEG C, 160 r/min vibrations
30min, with dilution the method for falling ware measure detergent to Escherichia coli, staphylococcus aureus, Candida albicans killing logarithm value,
Parallel test in triplicate, calculates bactericidal effect such as following table.
2. detergency experiment
Press《Manual dishwashing detergent》The method of the standards of GB 9985-2000 purchase artificial dirt, 250 ppm hard water,
Instrument needed for dirt ceramics plate and foam level method test detergency.Using foam end point method, respectively Example 1-5 with
And comparative example 1-6 liquid detergent 5mL, by 1000mL concentration for the hard water of 250ppm is poured into enamel basin, separately by 1000mL concentration
For the hard water of 250ppm is put into tubualted bottle.In room temperature（25 ℃）Under, the delivery port of tubualted bottle is opened, hard water is rushed in flowing into basin
Spume.After hard water stops flowing out（The s of process about 45）, plate is scrubbed with 102 mm pigs palm fibre paintbrush, first brush clockwise
5 times, another mistake hour hands brush 5 times, after so repeating 1 time, the dirt major part being applied on plate is washed down, finally again will not
Wash lower part to scrub off, until foam is wholly absent, record the number that washes dishes, and be finally converted into deep bid subnumber, it is as a result as follows
Shown in table.
3. security toxicological test.
The main formula of Example 1 carries out toxicological test, foundation respectively《Foodsafety Toxicological Assessment program and side
Method》GB 15193-2003, using maximum tolerance metering experiment method, dosage is set to 10000 mg/kg BW, from 20
Kunming mice, male and female half and half.The h of animal fasting 16, drinking-water is not limited before experiment.The mg/mL of animal 500 is given using administration by gavage
The sample solution of concentration, gavage amount is 0.4 mL/20 g body weight.The poisoning manifestations and death condition of animal are observed after gavage,
Weigh weekly 1 time, observe 14 d, it is as a result as shown in the table.
| Sample ID | Stearoyl citric acid | Stonewort tunning | Leaf of Magnolia officinalis extracts product |
| LD50（mg/kg·BW） | 20000 | 40000 | 35000 |
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, and do not carrying on the back
In the case of spirit or essential attributes of the invention, the present invention can be in other specific forms realized.Therefore, no matter from which
From the point of view of a bit, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention will by appended right
Ask and limited rather than described above, it is intended that all changes that will fall in the implication and scope of the equivalency of claim
Include in the present invention.
Moreover, it will be appreciated that although the present specification is described in terms of embodiments, not each implementation method is only wrapped
Containing an independent technical scheme, this narrating mode of specification is only that for clarity, those skilled in the art should
Specification an as entirety, the technical scheme in each embodiment can also be formed into those skilled in the art through appropriately combined
May be appreciated other embodiment.The ins and outs not described in detail in the present invention, can be by any in this area
Prior art is realized.Particularly, all technical characterstics not described in detail can be realized by any prior art in the present invention.
Claims (5)
1. a kind of Food-grade detergent, it is characterised in that by following parts by weight into being grouped into：Stearoyl citric acid 44-62
Part, deionized water 33-47 parts, sodium stearyl fumarate 12-23 parts, 2-5 parts of stonewort tunning, grape skin extract 3-7
Part, Leaf of Magnolia officinalis extracts 14-27 parts of product.
2. Food-grade detergent according to claim 1, it is characterised in that by following parts by weight into being grouped into：Firmly
51-58 parts of acyl citric acid, deionized water 37-43 parts, sodium stearyl fumarate 17-21 parts, 3-4 parts of stonewort tunning,
Grape skin extract 4-5 parts, Leaf of Magnolia officinalis extracts 18-25 parts of product.
3. Food-grade detergent according to claim 1, it is characterised in that the preparation method of the stonewort tunning
For：It is solid-liquid ratio 1: 10 to impose a condition（m/V）, cellulase Cellulase ACCF-4740 additions 2%, 55 DEG C of temperature,
PH 4.5, the h of reaction time 1.3；After cellulose hydrolyzation terminates, boiling water bath is gone out enzyme 15 min, and 9000 r/min centrifugations are collected
Supernatant obtains stonewort cellulose hydrolyzation liquid；Stonewort enzymolysis liquid goes out after enzyme activity, and to 6.2-6.6, high steam goes out regulation pH value
Bacterium（121 DEG C, 20 min）, the Pediococcus pentosaceus liquid that then the MRS culture mediums of inoculation volume fraction 1% are activated to stonewort enzymolysis
Liquid, 37 DEG C of constant incubators stand, and are fermented；After fermentation ends, high pressure steam sterilization, 9000 r/min centrifugations are collected
Supernatant freeze-drying, obtains stonewort tunning, is saved backup under the conditions of -20 DEG C.
4. Food-grade detergent according to claim 1, it is characterised in that the Leaf of Magnolia officinalis extracts the preparation method of product
For：Leaf of Magnolia officinalis is cleaned into stripping and slicing to dry, then Leaf of Magnolia officinalis grind into powder and crosses 50 mesh sieves, the Leaf of Magnolia officinalis powder after sieving
70% ethanol is added, the quality (g) of Leaf of Magnolia officinalis powder is with the volume (mL) of ethanol than m: V=1: 13；Ultrasonic wave shakes
Swing instrument (40 DEG C, 450 W) min of assisted extraction 20；Leaf of Magnolia officinalis ethanol flows back through water-bath extract again, and extraction temperature is 65
DEG C, extracting 3 times, 1.5 h every time, suction filtration centrifugation (5000r/min, 15min), rotary evaporation in vacuo is returned after filtering
Ethanol is received, Leaf of Magnolia officinalis crude extract is obtained；HPD-300 macroreticular resins are soaked into 24 h with 95% ethanol, distilled water is cleaned to nothing
20 cm × 240 special the chromatographic columns of cm macroreticular resins are put into after ethanol flavor；Leaf of Magnolia officinalis crude extract is with 400 BV/h (BV:
The volume of macroreticular resin) the rate of adsorption be adsorbed on HPD-300 macroreticular resins, upper column quantity be 600 BV；Adsorb
The resin distilled water of 800 BV with the impurity on the flow velocity eluted resin of 1000 BV/h, then with 1200 BV's
60% ethanol is eluted to the crude extract being attached on resin, and obtaining Leaf of Magnolia officinalis after wash-out liquid condensing concentration extracts product.
5. the preparation method of a kind of Food-grade detergent as described in any one of claim 1- 4, it is characterised in that including with
Lower specific steps：By in deionized water addition reactor, 70 DEG C are heated to, stearoyl-fumarate is sequentially added by weight
Sodium, after stearoyl citric acid stirs, temperature is down to 40 DEG C, and the pH values of solution are adjusted into 6.9, adds stonewort tunning,
Grape skin extract, Leaf of Magnolia officinalis extracts product and is stirred until homogeneous, and the feed liquid that will be stirred is de-gassed, and using vacuum outgas, bears
Voltage-controlled system is dispensed, you can obtain Food-grade detergent in 5Mpa to mixed liquor.
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| CN107090371A (en) * | 2017-06-19 | 2017-08-25 | 浩宇康宁健康科技（湖北）有限公司 | Vegetables and fruits cleaning agent and its preparation method and application |
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2016
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| CN107090371A (en) * | 2017-06-19 | 2017-08-25 | 浩宇康宁健康科技（湖北）有限公司 | Vegetables and fruits cleaning agent and its preparation method and application |
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