CN106717256A - A kind of tuber of pinellia stem tuber makes the method that bulbil is bred - Google Patents
A kind of tuber of pinellia stem tuber makes the method that bulbil is bred Download PDFInfo
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- CN106717256A CN106717256A CN201611062132.2A CN201611062132A CN106717256A CN 106717256 A CN106717256 A CN 106717256A CN 201611062132 A CN201611062132 A CN 201611062132A CN 106717256 A CN106717256 A CN 106717256A
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- tuber
- pinellia
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a kind of method that tuber of pinellia stem tuber makes bulbil propagation, step is：Treatment before A, immersion：First, from stem tuber；Second, bactericide immersion treatment is used, with 100,000 times of 70% weight than thiophanate methyl liquid immersion treatment；B, HORMONE TREATMENT stem tuber：In tuber of pinellia tissue cultures, cell division is added in the medium usually makes explant produce the stem tuber with bud, produces tuber of pinellia bulbil to breed by Thidiazuron immersion treatment tuber of pinellia stem tuber, and Thidiazuron concentration is 4mg/L, and soak time is 72 84h days；C, stem tuber planting soil and environment conditioning by hormone immersion treatment：Turfy soil, oily sandy soil, black sand are mixed, a kind of mixture are obtained, then is added composite fertilizer 30g to measure by 1 square meter and add composite fertilizer, germination period temperature control between 20 28 DEG C, with two pin sunshade net sunshades.It is easy to implement the method, it is easy to operate, it is with low cost without sterile working.The same brood body of production, cost is tissue-culturing rapid propagation mode 3.4%.It is that a kind of clean Gao Xiao ﹑ of Jian are adapted to application.
Description
Technical field
The invention belongs to field of plant growing technology, the side that a kind of tuber of pinellia stem tuber breeds bulbil is more particularly to
Method, is adapted to the application in tuber of pinellia artificial cultivation.
Background technology
The tuber of pinellia (Pinellia ternata) is Araeceae Pinellia Tenore herbaceos perennial, 15-30 centimetres of plant height；Block
Stem is subsphaeroidal, 1-2 centimetres of diameter；Leaf comes from stem tuber top, and petiole is long 6-23 centimetres, and annual leaf is single leaf, and ovate is heart-shaped；
After 2-3, leaf is trifoliolate leaf.The tuber of pinellia is a kind of conventional Chinese medicine, and in 588 kinds of prescriptions of traditional Chinese medicine, tuber of pinellia frequency of use is occupied
22nd.Have functions that stopping nausea and vomiting by lowering the adverse flow of QI, the eliminating dampness and eliminating phlegm, ruffian that disappears dissipate section.In recent years, research finds that the tuber of pinellia also has Robust speaker feature, resists
The functions such as tumour, reducing blood lipid, protect liver.Due to the huge market demand, Wild Rhizoma Pinelliae resource cannot meet, and there is now large-scale people
Work post is planted.But it is big that stem amount is planted needed for tuber of pinellia artificial growth, high cost.At present, every mu of ground of the artificial growth tuber of pinellia need to plant stem 300-
400kg, buys these kind of cost of stem about 6000-8000 unit, therefore, the cost of the summer sowing stem of artificial growth half is 6000-
8000 yuan/mu.This is also one of principal element of influence tuber of pinellia artificial growth benefit.Therefore, exploitation kind stem efficient propagation method
It is one of approach of raising tuber of pinellia artificial growth benefit.
Bulbil is the main brood body of the tuber of pinellia, and it life in petiole lower inside surface, and some meetings raw in three leaf intersections
One bulbil.Bulbil forms new plant in falling within soil, and bulbil becomes stem tuber.Under nature, the tuber of pinellia relies primarily on bulbil breeding.
To improve tuber of pinellia reproductive efficiency, the method for tuber of pinellia tissue culture propagation breeding is also widely studied.By the method for tissue culture, finally may be used
Half summer sporelings (of laminaria) is obtained, tuber of pinellia stem tuber can be also obtained, it should say, the method for tuber of pinellia tissue-culturing rapid propagation can increase substantially the breeding of the tuber of pinellia
Efficiency.But tissue culture method cannot replace at present using stem tuber as the method for kind of stem, and this is primarily due to tissue culture method high expensive,
And if being planted in tissue-cultured seedling form, can't harvest for 1 year.The shortcoming of these tissue culture methods limits the application of the tissue culture tuber of pinellia.
The content of the invention
The purpose of the present invention is to there are provided a kind of method that tuber of pinellia stem tuber breeds bulbil, easy to implement the method, operation letter
Just, it is with low cost without sterile working.The same brood body of production, its cost is about tissue-culturing rapid propagation mode 3.4%.It is a kind of
The clean Gao Xiao ﹑ of Jian are adapted to application.
In order to realize above-mentioned purpose, the present invention uses following technical measures：
Its technology design is：The present invention provides complete set and the adaptable bulbil paraphyte of the production of tuber of pinellia cultivation at present
System.Including three parts, Part I, the treatment before the immersion of tuber of pinellia stem tuber；Part II, the treatment of tuber of pinellia tuberous plant hormone solution
Method；Part III, stem tuber planting soil and environment conditioning.
A kind of tuber of pinellia stem tuber makes the method that bulbil is bred, its step be：
Treatment before A, immersion.Rotted to reduce the tuber of pinellia stem tuber during immersion treatment, mainly take two measures, the
One, must be healthy stem tuber from stem tuber, it is important to note that stem tuber bud point, it is impossible to occur rotting；Second, at bactericide immersion
Reason.Through overtesting, find with 100,000 times of 70% (active constituent content, weight ratio) thiophanate methyl liquid immersion treatment 14-16 points
Clock effect is preferable.According to tuber of pinellia stem tuber Perishing prevention pertinent literature, the present invention mainly employs carbendazim and thiophanate methyl and carries out
Compare.
B, HORMONE TREATMENT stem tuber.In tuber of pinellia tissue cultures, generally addition cell division usually makes explant in the medium
Body produces the stem tuber with bud.Groped by some experiments, found under non-condition of tissue culture, by Thidiazuron (TDZ) immersion treatment
Tuber of pinellia stem tuber can produce the effect that tuber of pinellia bulbil is bred.In order to determine Thidiazuron (TDZ) concentration and soak time, design gradient examination
Test, with TDZ 2.0mg/L or 4.0mg/L or 8.0mg/L or 16.0mg/L soak respectively stem tuber 72 or 74 or 76 or 78 or 80 or
82 or 84h., by experiment, Thidiazuron (TDZ) concentration is 4mg/L, and soak time is 72-84h days.Soak time is oversize to be led
Stem tuber is caused to rot to aggravate, too short effect is not obvious.
The described basic element of cell division (cytokinin, CTK) is separated or artificial synthesized plant from corn or other plant
Hormone.It is general to be produced in plant root, it is the material of class promotion cytokinesis, promote the differentiation and growth of Various Tissues.With
Plant growth have synergy.It is the plant hormone for adjusting plant cell growth and development.Activation is played in cell division to make
With, be also contained in cell growth to differentiation and other related physiological activities during, such as kinetin (KT), zeatin (ZT),
6- benzylaminopurines (6-BA) etc..
C, stem tuber planting soil and environment conditioning by hormone immersion treatment.Through experiment, it is found that following soil is matched somebody with somebody and compare
It is good：20% (volume ratio) turfy soil+40% (volume ratio) oil sandy soil+40% (volume ratio) black sand, by turfy soil, oily sandy soil, black
Sand is mixed, and obtains a kind of mixture, then add composite fertilizer 30g to measure addition composite fertilizer by 1 square meter.Ensure suitable moisture, germination
Period temperature control between 20-28 DEG C, with two pin sunshade net sunshades.After hormone immersion treatment, can the tuber of pinellia produce more
Bulbil, also depends on the nutrition of abundance and suitable environmental condition.Under above-mentioned matrix and environmental condition, the tuber of pinellia bulbil propagation compared with
It is many, and bulbil is full, quality is higher.
Described compound fertilizer refers to the chemical fertilizer containing two or more nutrient, and composite fertilizer has nutrient content
The advantages of high, few accessory ingredient and good physical behavior, for balance fertilizing, utilization rate of fertilizer is improved, promote the stable high yield of crop
There is highly important effect.But it also has some shortcomings, such as its nutrients ratio is always fixed, and different soils, no
It is various with the nutrient species needed for crop, quantity and ratio.Therefore, preferably carry out surveying soil using preceding, understand field
The quality and nutrition condition of soil, further note also that with straight fertilizer compounding application, can just obtain more preferable effect.
The present invention compared with prior art, with advantages below and effect：
1. compared with tuber of pinellia natural propagation, the present invention can improve 3-4 times of reproductive efficiency.
2. compared with tissue-culturing rapid propagation, the present invention is simple to operate, with low cost.The same brood body of production, its cost is about
Tissue-culturing rapid propagation mode 3.4%.
3. the present invention can be synchronous with tuber of pinellia cultivation, does not influence the tuber of pinellia to cultivate.
4. the present invention matches with current tuber of pinellia planting type.The operation links such as tissue-culturing rapid propagation transplantation of seedlings can be reduced, can
Avoid the problem that tissue-cultured seedling stem tuber is too small, growth cycle is long.
5. the present invention is simple to operate, and staff is easily mastered, and possesses promotion prospect.
Specific embodiment
The present invention is described in further detail with reference to embodiments, but is not limitation of the present invention, it is all according to
The equivalent of any this area made according to the disclosure of invention, belongs to protection scope of the present invention.
Embodiment 1：
A kind of tuber of pinellia stem tuber makes the method that bulbil is bred, its step be：
Treatment before A, immersion.Rotted to reduce the tuber of pinellia stem tuber during immersion treatment, mainly take two measures, the
One, must be healthy stem tuber from stem tuber, it is important to note that stem tuber bud point, it is impossible to occur rotting；Second, at bactericide immersion
Reason.Through overtesting, find with 100,000 times of 70% (active constituent content, weight ratio) thiophanate methyl liquid immersion treatment 14 or 15 or
Effect is preferable within 16 minutes.Bactericide species is various, mainly employs carbendazim and thiophanate methyl, according to pertinent literature, mainly
Compare carbendazim and thiophanate methyl is compared experiment.
Concrete operation step is：Diameter is selected in more than 1.5cm, healthy tuber of pinellia stem tuber.With 100,000 times 70% (weight ratio,
It is same as below) thiophanate methyl liquid immersion treatment 15 minutes.Then thiophanate methyl liquid is outwelled.By water under room temperature (20-25 DEG C)
Dry.To understand influence of the different disposal method to follow-up hormone immersion treatment, design experiment the results are shown in Table 1.Each treatment takes
100 healthy stem tubers, by after different disposal, adding hormone solution immersion, counted rotten every 12h since 48h hours
Rate.Rotting rate=rot stem tuber number/100 × 100%.As it can be seen from table 1 with 100,000 times of 70% thiophanate methyl immersion bubble
Reason can reduce rotting rate in subsequent processes in 15 minutes.
B, HORMONE TREATMENT stem tuber.In tuber of pinellia tissue cultures, generally addition cell division usually makes explant in the medium
Body produces the stem tuber with bud.Groped by some experiments, found under non-condition of tissue culture, by Thidiazuron (TDZ) immersion treatment
Tuber of pinellia stem tuber can produce the effect that tuber of pinellia bulbil is bred.But, soak time is oversize to cause stem tuber to rot to aggravate, too short effect
It is unobvious.In order to determine TDZ concentration and soak time, distinguished with TDZ2.0mg/L or 4.0mg/L or 8.0mg/L or 16.0mg/L
Immersion stem tuber 72 or 74 or 76 or 78 or 80 or 82 or 84h.
Hormone, Greek original meaning is " rising vigorously activity ", its metabolism to body, growth, development, breeding, sex, sexual desire and
Sexuality etc. plays important adjustment effect.Hormone is well differentiated endocrine cell synthesis and is directly secreted into the chemistry letter of blood
Breath material, it influences the physiological activity of human body by adjusting various histiocytic metabolic activities.By incretory or interior point
The efficient bio-active material of cell secretion is secreted, in vivo as courier's transmission information, organism physiology process is played regulatory role
Material be referred to as hormone.It is the important substance in our life.
Described hormone is plant hormone, and plant hormone mainly includes：The Chang Su ﹑ Xi born of the same parents division Su ﹑ Chi Mei Su ﹑ abscisic acids of Sheng
And ethene.
The described basic element of cell division mainly includes：Kinetin (KT) ﹑ zeatin (ZT) ﹑ 6- benzylaminopurines (6-BA) ﹑
Thidiazuron (TDZ).
C, stem tuber planting soil and environment conditioning by hormone immersion treatment.Through experiment, it is found that following soil is matched somebody with somebody and compare
It is good：20% (volume ratio) turfy soil+40% (volume ratio) oil sandy soil+40% (volume ratio) black sand, by turfy soil, oily sandy soil, black
Sand is mixed, and obtains a kind of mixture, then add composite fertilizer 30g to measure addition composite fertilizer by 1 square meter.Ensure suitable moisture, germination
Period temperature control between 20 or 23 or 26 or 28 DEG C, with two pin sunshade net sunshades.
Diameter is selected in more than 1.5cm, healthy tuber of pinellia stem tuber.With 100,000 times of 70% 15 points of thiophanate methyl liquid immersion treatment
Clock.Then thiophanate methyl liquid is outwelled.Water is dried at room temperature.To understand different disposal method to follow-up hormone immersion treatment
Influence, design experiment the results are shown in Table 1.Each treatment takes 100 healthy stem tubers, by after different disposal, adding hormone solution leaching
Bubble, rotting rate was counted since 48h hours every 12h.Rotting rate=rot stem tuber number/100*100%.Can from table 1
Go out, rotting rate in subsequent processes can be reduced within 15 minutes with 100,000 times of 70% thiophanate methyl liquid immersion treatment.
The influence that the different disposal of table 1 is rotted to tuber of pinellia stem tuber immersion treatment
Embodiment 2：
A kind of tuber of pinellia stem tuber makes the method that bulbil is bred, its step be：
The first step, TDZ 1mg/mL solution is prepared.0.1gTDZ is weighed, is first dissolved with 4% (g/mL) sodium hydroxide solution,
Then constant volume is added water in 100mL.
Second step, 4mg/L is diluted to by TDZ solution, and keep pH value 5.8 or 6 or 6.2 or 6.4 or 6.6 or 6.8 or
Between 7.0.
3rd step, the TDZ solution that will be prepared is poured into through 100,000 times of 70% (active constituent content, weight ratio) methyl Tobe
In stem tuber after body fluid immersion treatment 14 or treatment in 15 or 16 minutes, immersion stem tuber 72 hours.In order to verify various concentrations hormone
The influence to cultivation effect is processed, design experiment, result of the test is shown in Table 2.Bulbil proliferation times computational methods：10 stem tubers are taken,
The bulbil that grows of the tuber of pinellia is divided by control after being soaked with TDZ hormones, i.e., not by HORMONE TREATMENT, produced bulbil.Rot block
Stem does not count.From table 2 it can be seen that as TDZ concentration and soak time increase, proliferation times increase.But over time
Increase, rotting rate is also stepped up.Consider, it is preferable with 4mg/L TDZ immersion treatments 72h.
The influence that the hormone immersion treatment of table 2 is bred to bulbil.
Other implementation steps are same as Example 1.
Embodiment 3：
A kind of tuber of pinellia stem tuber makes the method that bulbil is bred, its step be：
The first step, is matched somebody with somebody by formula 20% (volume ratio) turfy soil+40% (volume ratio) oil sandy soil+40% (volume ratio) black sand
Soil is made, turfy soil, oily sandy soil, black sand are mixed, obtain a kind of mixture, then composite fertilizer is added by 30g/ square meters.
Second step, by above-mentioned immersion treatment stem tuber plantation to above-mentioned soil, waters, and temperature is maintained at 22-28 DEG C, illumination
Intensity is maintained at natural lighting intensity 60%.
Other implementation steps are same as Example 1.
Claims (1)
1. a kind of tuber of pinellia stem tuber makes the method that bulbil is bred, its step be：
Treatment before A, immersion：First, from stem tuber, it is impossible to occur rotting；Second, bactericide immersion treatment is used, with 100,000 times
70% weight was than thiophanate methyl liquid immersion treatment 14-16 minutes；
B, HORMONE TREATMENT stem tuber：In tuber of pinellia tissue cultures, cell division is added in the medium usually makes explant produce band
The stem tuber of bud, produces tuber of pinellia bulbil to breed, in order to determine Thidiazuron concentration and immersion by Thidiazuron immersion treatment tuber of pinellia stem tuber
Time, gradient test is designed, Thidiazuron concentration is 4mg/L, and soak time is 72-84h days；
C, stem tuber planting soil and environment conditioning by hormone immersion treatment：Following soil proportioning：20% volume ratio turfy soil+
The 40% volume ratio oil black sand of volume ratio of sandy soil+40%, turfy soil, oily sandy soil, black sand are mixed, and obtain a kind of mixture,
Add composite fertilizer 30g to measure by 1 square meter again and add composite fertilizer, germination period temperature control is hidden between 20-28 DEG C with two pin sunshade nets
Sun.
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| CN201611062132.2A CN106717256A (en) | 2016-11-22 | 2016-11-22 | A kind of tuber of pinellia stem tuber makes the method that bulbil is bred |
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| CN201611062132.2A CN106717256A (en) | 2016-11-22 | 2016-11-22 | A kind of tuber of pinellia stem tuber makes the method that bulbil is bred |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101288370A (en) * | 2008-06-13 | 2008-10-22 | 李敏 | Pinellia ternate good quality and high output culture technique |
| GB2519638A (en) * | 2013-08-23 | 2015-04-29 | Fine Agrochemicals Ltd | Growth regulator concentrate and use thereof |
-
2016
- 2016-11-22 CN CN201611062132.2A patent/CN106717256A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101288370A (en) * | 2008-06-13 | 2008-10-22 | 李敏 | Pinellia ternate good quality and high output culture technique |
| GB2519638A (en) * | 2013-08-23 | 2015-04-29 | Fine Agrochemicals Ltd | Growth regulator concentrate and use thereof |
Non-Patent Citations (5)
| Title |
|---|
| 卢正言: "《养花一得》", 31 July 1995, 上海书店出版社 * |
| 孙新荣，等: "半夏块茎腐烂病病原鉴定和药效比较", 《中国中药杂志》 * |
| 朱国胜 等: "《贵州半夏研究》", 31 December 2014, 贵州民族出版社 * |
| 胡鹏: "贵州珍珠半夏的离体培养及遗传稳定性的分子检测", 《中国优秀硕士学位论文全文数据库农业科技辑》 * |
| 阿里穆斯 等: "《少数民族药用植物学野外实践与学习指导》", 30 June 2013, 中央民族大学出版社 * |
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