CN106701643A - Fast decomposition agent for livestock and poultry excrement waste and preparation method and application thereof - Google Patents
Fast decomposition agent for livestock and poultry excrement waste and preparation method and application thereof Download PDFInfo
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- CN106701643A CN106701643A CN201710157863.3A CN201710157863A CN106701643A CN 106701643 A CN106701643 A CN 106701643A CN 201710157863 A CN201710157863 A CN 201710157863A CN 106701643 A CN106701643 A CN 106701643A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Abstract
The invention relates to a fast decomposition agent for livestock and poultry excrement waste and a preparation method and application thereof. The fast decomposition agent is prepared from bacillus macerans powder, geobacillus stearothermophilus powder, burkholderia cenocepacia powder, nocardia amarae powder and coprinus sterqulinus powder according to a mass ratio of 2 to 3 to 2 to 2 to 2. Through controlling the microbial fermentation process and the proportion of the mutual mutualistic symbiosis, metabiosis and non-antagonism effect of traditional Chinese medicine ingredients, the total effective live bacterium number in the decomposition agent is as high as (2 to 2.5)*10<8>/g; the enzyme activity is doubled; the product quality performance is stable; the moisture content of all microbial bacteria is controlled to be within 10 percent through spraying drying; the shelf life of a product is greatly prolonged; the storage and the transportation are facilitated; better popularization and use values are realized.
Description
Technical field
The invention belongs to microbial preparation field, it is related to a kind of for the quick composting agent of livestock and poultry feces discarded object and its system
Preparation Method and application.
Background technology
The annual various livestock and poultry feces waste productions of China are up to billions of tons, are discarded to process these livestock and poultry feces
Thing, many raisers are directly discharged to rivers,lakes and seas.Livestock and poultry feces discarded object not only pollutes environment, and serious waste
Resource, destruction is ecological.In order to fundamentally solve this contradiction, China has carried out " livestock and poultry feces recycling waste " aspect
Research, its main purpose is exactly that the hair of livestock and poultry feces discarded object is realized in selection using the livestock and poultry feces discarded object decomposing agent got well
Ferment carries out the production of biological organic fertilizer, realizes recycling for livestock and poultry feces waste material.
Increasingly increase with the common people to animal products demand, China's annual speed with more than 10% of livestock and poultry cultivation amount increases
It is long, and it is gradually concentrated in economically developed, traffic convenience, the market demand big big and medium-sized cities and coastal area.But it is simultaneously a large amount of to raise
Brid guano dirt discarded object but can not effectively be disposed and utilized, and trigger a series of environment and ecological problem, such as cause water body face
Source pollution aggravates, pollutant in soil is accumulated etc., has caused the public and government's common concern.State Council of the Central Committee of the Communist Party of China one in 2013
Number file has reiterated that " intensive farming production process environmental monitoring, actively develops widespread pollution from the overuse of fertilizers and pesticides in rural area and Pollution from livestock and poultry
Preventing and treating " work.Therefore, on the premise of ensuring livestock and poultry cultivation industry effective development, ensuring farmers' income, effectively disposal, profit
Pollution risk with livestock and poultry feces discarded object, reduction environment and ecology is extremely urgent.
At present, both at home and abroad in the treatment of livestock and poultry feces discarded object with Aerobic Process for Treatment (During High-Temperature Composting), Anaerobic Treatment (natural pond
Gas) and burning disposal (ashing) based on.China is using various technical finesse feces of livestock and poultry to realize minimizing, innoxious, money
The purpose in source, current China is concentrated mainly on following three kinds to the processing and treating method of feces of livestock and poultry：Fodder, energy,
Fertilizer Transformed etc..
Fodder：Not only contain a large amount of indigested protein, vitamin but also also a number of in feces of livestock and poultry
Glucide and abundant amino acid classes.In order to meet the supply of forage in animal husbandry market, develop with albumen higher
The feed resource of matter content and complete amino acid classes is current most top priority.Domestic and international the experimental results show, chicken
Excrement is not only the anti-protein supplement good when animal, is also nonruminant and the good forage protein source of fish, chicken manure
Have become a kind of unconventional water resources resource of greatest concern.
Energy：Energy has burning and two kinds of means of anaerobic fermentation.Feces of livestock and poultry is direct plungeed under special furnace fully
Burning turns into lime-ash, and the heat of generation can be used for generating etc..This method can make feces of livestock and poultry decrement 90% within a short period of time
More than, and kill the harmful levels of pathogens and worm's ovum in excrement.But investment is big, processing cost is expensive, and a large amount of titanium dioxides are discharged during burning
Carbon and other pernicious gases, have harmful effect to atmospheric environment, while some nutrients for having value are burned off, cause
The waste of regenerated resources.Current most study, it is most rising be that biogas is obtained by anaerobic digestion process.Anaerobic fermentation
Production biogas, for production and living provide the energy, while biogas residue and biogas slurry justice are good organic feed and fertilizer, is so both reduced
Pollution, improves the economic benefit of pollutant control again.But fairly large Anaerobic Treatment biogas residue and biogas liquid need to be deepened using
Can discharge.
Fertilizer Transformed：At present, it is standby mode popular to people using feces of livestock and poultry production organic fertilizer, not only solves pollution
Problem and can also to greatest extent changing waste into resources utilize.The high temperature that composting process is produced can kill the micro- life of a large amount of cause of diseases
Thing, weed seed and insect, make organic matter reach innoxious and resource utilization, in addition, what compost was produced after terminating
Organic fertilizer can also provide nutritional ingredient and the organic matters, the treasured as agricultural development such as required N, P, K for substantial amounts of crops
Your resource.Fertilizer Transformed is particularly suitable for promoting the economic model of breeding circulation, thoroughly to make the channel that output is cleared up in excrement dirt in order, makes poultry
The comprehensive utilization of poultry breeding industry production discarded object and dirty improvement of excrement are in a complete system engineering, with operating cost phase
The advantage flexible to relatively low, processing mode.
So decomposing agent treatment livestock and poultry feces discarded object production organic fertilizer turns into present new one kind efficiently, economical and environmentally friendly
Mode, the know-why of existing livestock and poultry feces discarded object decomposing agent is exactly the main component according to livestock and poultry feces discarded object：Not
The starch of digestion, albumen, cellulose, hemicellulose, lignin feature, screening are produced：Amylase, protease, cellulase, half
The microbial strains of cellulase, lignoenzyme, carry out microorganism complex technique PROCESS FOR TREATMENT, them is effectively compounded in one
Rise.But in existing produced decomposing agent product, most of decomposing agent products are both for livestock and poultry of being become thoroughly decomposed under the conditions of banking up
Excrement or its with livestock and poultry feces discarded object mixed material and the microbial inoculum product of development ＆ production, exist that product bacterium number content is low, enzyme
Quantity living is too small, it is difficult to meet being actually needed for market application, while the strain and strain of the selection of decomposing agent product are assembled endless
Rationally, could not take into full account between the enzymatic productivity and strain of strain itself that mutually there is symbiosis, alternate, antagonism pair
The influence of product.Especially fungal species are few, and the effect stability that becomes thoroughly decomposed of livestock and poultry feces discarded object is poor in use, speed is slow.
The content of the invention
In view of this, it is an object of the invention to provide a kind of for the quick composting agent of livestock and poultry feces discarded object and its system
Preparation Method and application.
To reach above-mentioned purpose, the present invention provides following technical scheme：
1. a kind of quick composting agent for livestock and poultry feces discarded object, the quick composting agent component is Bacillus macerans
Powder, Geobacillus stearothermophilus powder, bulkholderia cepasea powder, ditch Nocard's bacillus powder and excrement ghost umbrella powder；In the decomposing agent
Total living bacteria count is 2~2.5 × 108/g.
Further, Bacillus macerans powder, Geobacillus stearothermophilus powder, Burkholder in the quick composting agent
Salmonella powder, ditch Nocard's bacillus powder and excrement ghost umbrella powder are 2-5 in mass ratio：2-5：l-3：1-3：1-4.
Further, Bacillus macerans powder, Geobacillus stearothermophilus powder, Burkholder in the quick composting agent
Salmonella powder, ditch Nocard's bacillus powder and excrement ghost umbrella powder are 2 in mass ratio：3：2：2：2.
2. a kind of preparation method of quick composting agent for livestock and poultry feces discarded object is following steps：
(1) Bacillus macerans powder is prepared：Bacillus macerans strain is inoculated in activation culture in slant medium, so
After be transferred in eggplant-shape bottle after Amplification Culture to be seeded to fermentation cylinder for fermentation culture to spore forming rate and reach 90-95% and complete training
Support, 5~10 times of centrifugal concentrating zymotic fluid after fermentation, then it is spray-dried to Bacillus macerans powder；
(2) Geobacillus stearothermophilus powder is prepared：Geobacillus stearothermophilus strain is inoculated in slant medium
Middle activation culture, is then transferred to and is seeded to fermentation cylinder for fermentation culture to spore forming rate after Amplification Culture in eggplant-shape bottle and reaches
90-95% completes culture, 5~10 times of centrifugal concentrating zymotic fluid after fermentation, then spray-dried Geobacillus stearothermophilus
Powder；
(3) bulkholderia cepasea powder is prepared：Culture is inoculated into after bulkholderia cepasea conidia powder and flour are mixed
On based mixtures material, then koji tray cultivation and fermentation is carried out, through drying and crushing after fermentation completion, 100 mesh screen filtrations are obtained Bai Kehuo
That moral Salmonella powder；
(4) ditch Nocard's bacillus powder is prepared：Culture medium mixture is inoculated into after ditch Nocard's bacillus conidia powder and flour are mixed
On material, then koji tray cultivation and fermentation is carried out, through drying and crushing after fermentation completion, 100 mesh screen filtrations are obtained ditch Nocard's bacillus powder；
(5) excrement ghost umbrella powder is prepared：Chinese medicine excrement ghost umbrella is crushed, 100 mesh screen filtrations；
(6) Bacillus macerans powder, Geobacillus stearothermophilus powder, bulkholderia cepasea powder, ditch promise are taken by formula
Cattell bacterium powder and excrement ghost umbrella powder are well mixed, and obtain final product quick composting agent.
Further, the moisture content percentage by weight of the Bacillus macerans powder is 5-6%；Bacillus macerans powder is effectively lived
Bacterium number is 2~2.5 × 108Individual/g；The moisture content percentage by weight for the Geobacillus stearothermophilus powder is 5-6%；
Geobacillus stearothermophilus powder living bacteria count is 2~2.5 × 108Individual/g；The bulkholderia cepasea powder moisture content weight
Percentage is 5-6%；Bulkholderia cepasea powder living bacteria count is 2~2.5 × 108Individual/g；The ditch Nocard's bacillus
Powder moisture content weight percent content is 5-6%；Ditch Nocard's bacillus powder living bacteria count is 2~2.5 × 108Individual/g.
Further, step (1) and step (2) described activation culture are 30-37 DEG C and cultivate 24-40 hours；The fermentation training
Support is to be cultivated 8-36 hours under the conditions of 30-32 DEG C；Culture medium each component concentration is as follows in eggplant-shape bottle：Tryptone 8-10g/L,
Yeast extract 3-5g/L, sodium chloride 3-5g/L, solvent is water；Culture medium each component concentration is as follows in fermentation tank：Glucose
0.3-0.5g/L, beef extract 0.3-0.5g/L, peptone 0.3-0.5g/L and sodium chloride 0.3-0.5g/L, solvent is water.
Further, spray drying process is the addition concentrated broth weight in contracting zymotic fluid in step (1) and (2)
The precipitated calcium carbonate of 20%-40% simultaneously stirs, and is passed through spray drying tower and is dried, and intake air temperature is 195-
200 DEG C, air outlet temperature be 75-80 DEG C, until keep spray bacterium powder moisture content percentage by weight between 5-6%.
Further, step (3) and step (4) the culture medium mixture material are to take wheat bran, bean cake powder and corn flour by matter
Amount compares 60-70:15-20:15-20 is mixed into material, adds epsom salt, the quality of material of quality of material 0.3-0.5%
The ferric sulfate of 2.5-3.5%, quality of material 0.1-0.2% potassium dihydrogen phosphates, and add water make moisture be 50-60%；Institute
It is 25-30 DEG C to state koji tray cultivation and fermentation temperature, and fermentation time is 35-50 hours, long on culture medium mixture material when fermentation is finished
Full conidia powder.
Further, the relation 0.01-0.02 of the conidia powder, flour and culture medium mixture doses:1-2:1-2.
3. application of the quick composting agent in treatment livestock and poultry feces discarded object production organic fertilizer.
The beneficial effects of the present invention are：1. the present invention provide livestock and poultry feces discarded object decomposing agent with bacterium, fungi and
Chinese medicine excrement ghost umbrella powder rational proportion is constructed to the decomposing agent product for making livestock and poultry feces discarded object be converted into biological organic fertilizer, with pin
, heat up rapid, become thoroughly decomposed effect strong to property is good, capability and performance stabilization advantage.2. in the present invention, the livestock and poultry feces discarded object
Decomposing agent is answered by Bacillus macerans, Geobacillus stearothermophilus, bulkholderia cepasea, ditch Nocard's bacillus and excrement ghost umbrella powder
Combined group is formed, and has good symbiosis, mutuality between these strains and Chinese medicine excrement ghost umbrella powder, does not have antagonism.3.
The key technical indexes that livestock and poultry feces discarded object decomposing agent of the invention reaches：A. four kinds of function strains are used into liquid, solid
Double fermentation, is concentrated by being spray-dried, and greatly improves the density of microorganism, make product living bacteria count by original 1.0 hundred million/
G brings up to 200,000,000/more than g；Enzyme activity improves 1 times, it is ensured that product quality stable performance, using effect are good；B. zymotechnique is controlled
Link, makes function strain be completely formed the gemma and spore state of stabilization, and spray drying link makes livestock and poultry feces discarded object become thoroughly decomposed
The water content of agent product can be controlled within 10% substantially, significantly extend shelf life of products, beneficial to storage, transport, had
More preferable promotion and use value；C. strain assembles collective effect has clear and definite directionality, to reach mutual cooperation, collaboration row
Fight, the purpose of quick composting livestock and poultry feces discarded object reduces production cost, is easy to promote, uses.
Brief description of the drawings
In order that the purpose of the present invention, technical scheme and beneficial effect are clearer, the present invention provides drawings described below and carries out
Explanation：
Fig. 1 is the quick composting agent process flow diagram for livestock and poultry feces discarded object.
Specific embodiment
Below in conjunction with accompanying drawing, the preferred embodiments of the present invention are described in detail.It is unreceipted specific in embodiment
The experimental technique of condition, generally according to conventional laboratory conditions or according to the condition proposed by manufacturer.
Embodiment 1 prepares Bacillus macerans powder：
A, lyophilized Bacillus macerans strain is transferred on preprepared test tube slant in gnotobasis, temperature
At 35 DEG C, culture is transferred to eggplant type bottle culture 30 hours again after 36 hours, check and confirm without being connect as fermentation tank after miscellaneous bacteria for degree control
Kind；Culture medium each component concentration is as follows in eggplant-shape bottle：Tryptone 8-10g/L, yeast extract 3-5g/L, sodium chloride 3-5g/
L, solvent is water；
B, prepare culture medium material：Culture medium material need to use glucose 0.5g, beef extract according to every liter of water in fermentation tank
The ratio of 0.5g, peptone 0.5g and sodium chloride 0.5g is matched, and is mixed together soluble in water, is delivered in fermentation tank；
C, sterilizing cooling：To steam is passed through in fermentation tank, stirring is opened, treat that culture medium temperature of charge reaches 121 DEG C in tank
Shi Baowen 30 minutes, closes steam after the completion of sterilizing, opening cooling system makes the temperature of culture medium material be down to 30-35 DEG C；
D, inoculation：Take eggplant type bottle of the inspection obtained in step A without miscellaneous bacteria several, according to culture medium material body in fermentation tank
Product accesses the ready fermentation tank of step C based on every cubic metre of culture medium material inoculation 1000ML strain by sterile working method
In；
E, fermented and cultured；30 DEG C of filtrated airs are passed through in D steps and are inoculated with the fermentation tank for finishing, for Spawn incubation is provided
Oxygen, after cultivating 8 hours, starts to sample microscopy, is taken a sample to check within every 4 hours when fermenting 8 to 24 hours secondary, ferments 24-36 hours
When every 2 hours check once, it is ensured that without miscellaneous bacteria, when spore forming rate reaches 90-95% cultivate complete；
F, centrifugation：The zymotic fluid obtained in E steps is carried out 5-10 times by centrifugal separator to concentrate, as concentration
Zymotic fluid；
G, allotment spray drying：The concentrated broth that will be obtained in F-step is squeezed into blend tank, adds concentrated broth weight
The precipitated calcium carbonate of amount 30% is stirred, and is then passed through spray drying tower and is dried, and intake air temperature is controlled 195
DEG C, air outlet temperature control at 75 DEG C, until keep spray Bacillus macerans powder moisture content weight percent content in 5-
Between 6%；
H, Bacillus macerans powder are processed for depositing stand-by：By the Bacillus macerans at spray drying tower bottom of towe and whirlwind mouthful
Powder is mixed, and sealing preserve is standby after being well mixed；Bacillus macerans powder living bacteria count is 2~2.5 × 108Individual/g.
Embodiment 2 prepares Geobacillus stearothermophilus powder
A, lyophilized Geobacillus stearothermophilus strain is transferred to preprepared test tube slant in gnotobasis
On, by temperature control at 30-35 DEG C, culture is transferred to eggplant type bottle culture 32 hours for 36 hours again, checks and confirms without being used as after miscellaneous bacteria
Fermentation tank is inoculated with；Culture medium each component concentration is as follows in eggplant-shape bottle：Tryptone 8-10g/L, yeast extract 3-5g/L, chlorination
Sodium 3-5g/L, solvent is water；
B, prepare culture medium material：Culture medium material need to use glucose 5g, yeast extract 2g, egg according to every liter of water in fermentation tank
White peptone lg, the proportioning of bean cake powder 5g and potassium dihydrogen phosphate 0.5g are configured, in it is dissolved in water, in feeding fermentation tank；
C, sterilizing cooling：To steam is passed through in fermentation tank, stirring is opened, treat that culture medium temperature of charge reaches 121 DEG C in tank
When, 30 minutes are incubated, steam is closed after the completion of sterilizing, opening cooling system makes the temperature of culture medium material be down to 32 DEG C；
D, inoculation：Take the eggplant type equipped with Geobacillus stearothermophilus on inspection without miscellaneous bacteria bottle obtained in step A some
It is individual, according to culture medium material in fermentation tank, based on every cubic metre of culture medium material seed dressing 1000ML strain, connect by sterile working method
In entering the ready fermentation tank of step C；
E, fermented and cultured：30 DEG C of filtrated airs are passed through in D steps and are inoculated with the fermentation tank for finishing, for Spawn incubation is provided
Oxygen, after cultivating 8 hours, starts to sample microscopy, is taken a sample to check once within every 4 hours when fermenting 8 to 24 hours, and 24-36 is small for fermentation
Constantly check once within every 2 hours, it is ensured that without miscellaneous bacteria, culture is completed after quantity stabilization；
F, centrifugation：The zymotic fluid obtained in E steps is carried out 5-10 times by centrifugal separator to concentrate, as concentration
Zymotic fluid；
G, spray drying：The concentrated broth that will be obtained in F-step is squeezed into blend tank, adds concentrated broth weight
30% precipitated calcium carbonate stirs, and the zymotic fluid for stirring is dried by spray drying tower, intake air temperature
At 195 DEG C, air outlet temperature is controlled at 75 DEG C for control, the moisture content weight hundred until keeping spraying Geobacillus stearothermophilus powder
Point ratio is between 5-6%；
H, Geobacillus stearothermophilus powder are processed for depositing stand-by：By the stearothermophilus ground at spray tower bottom of towe and whirlwind mouthful
Bacillus powder is mixed, and sealing preserve is standby after being well mixed.Geobacillus stearothermophilus powder living bacteria count be 2~
2.5×108Individual/g.
Embodiment 3 prepares bulkholderia cepasea powder：
A, preparation culture medium mixture material：Matched according to mass percent, wherein the proportioning of material is wheat bran 70%, dregs of beans
Powder 15%, corn flour 15%, are mixed in feeding fermentation tank, and the seven water sulfuric acid for accounting for quality of material 0.5% are added in material
Magnesium, the ferric sulfate for accounting for quality of material 3.5%, the potassium dihydrogen phosphate of quality of material 0.2% is accounted for, and add distilled water, make moisture
It is 60%, wherein trace element is added in the lump with after water dissolves with water；
B, sterilizing cooling：Culture medium mixture material to being obtained in step A is warming up to 121 DEG C, and 121 DEG C of pressurizes 1 of insulation are small
Shi Hou, starts, to fermentation tank interlayer ventilating and cooling, to be cooled to 30 DEG C；
C, inoculation：After bulkholderia cepasea conidia powder and lkg flour are mixed, be inoculated into step B obtain through height
It is inoculated with culture medium mixture material in fermentation tank after warm autoclave sterilization；The mixing of the conidia powder, flour and culture medium
The mass ratio of inventory is 0.01-0.02:1-2:1-2.
D, koji tray culture：The mixed material of the step C being inoculated with after bulkholderia cepasea is put into aseptic fermenting cellar
Diameter on 1.2 meters of koji tray, often paved by disk, thickness 6-10cm, and temperature of charge is controlled at 30 DEG C, is carried out when temperature is more than 30 DEG C
Turning, aeration-cooling oxygenating, fermentation time are 35-50 hours, until covering with black or brown on the culture medium mixture material for fermenting
Color conidia powder, fermentation is completed；
E, drying：By the bulkholderia cepasea conidia powder after fermentation intake air temperature be 195-200 DEG C, air outlet temperature
Spend to be dried at 75-80 DEG C, it is 2~2.5 × 10 that drying finishes rear bulkholderia cepasea powder living bacteria count8Individual/g.
Embodiment 4 prepares ditch Nocard's bacillus powder：
A, preparation culture medium mixture material：Matched according to mass percent, wherein the proportioning of material is wheat bran 70%, dregs of beans
Powder 15%, corn flour 15%, are mixed in feeding fermentation tank, and the seven water sulfuric acid for accounting for quality of material 0.5% are added in material
Magnesium, the ferric sulfate for accounting for quality of material 3.5%, the potassium dihydrogen phosphate of quality of material 0.2% is accounted for, and add distilled water, make moisture
Up to 60%, wherein trace element is added in the lump with after water dissolves with water；
B, sterilizing cooling：Culture medium mixture material to being obtained in step A is warming up to 121 DEG C, and 121 DEG C of pressurizes 1 of insulation are small
Shi Hou, starts, to fermentation tank interlayer ventilating and cooling, to be cooled to 30 DEG C；
C, inoculation：After ditch Nocard's bacillus conidia powder and lkg flour are mixed, obtain high through high temperature is inoculated into step B
It is inoculated with the culture medium mixture material in fermentation tank after pressure sterilization；The conidia powder, flour and culture medium mixture material
The mass ratio of amount is 0.01-0.02:1-2:1-2.
D, koji tray culture：The mixed material of the step C being inoculated with after ditch Nocard's bacillus is put into the diameter in aseptic fermenting cellar
On 1.2 meters of koji tray, often disk is paved, thickness 6-10Cm, and temperature of charge is controlled at 30 DEG C, is turned over when temperature is more than 30 DEG C
Heap, aeration-cooling oxygenating, fermentation time are 45 hours, until covering with green spores powder on the culture medium mixture material for fermenting, are sent out
Ferment is completed；
E, by the ditch Nocard's bacillus conidia powder after fermentation intake air temperature be 195-200 DEG C, air outlet temperature is 75-80
Dried at DEG C, it is 2~2.5 × 10 that drying finishes rear ditch Nocard's bacillus powder living bacteria count8Individual/g.
The excrement of embodiment 5 ghost umbrella powder
Chinese medicine excrement ghost umbrella is crushed, it is standby that 100 mesh screen filtrations are made excrement ghost umbrella powder；
The decomposing agent of embodiment 6
Take 2 kilograms of Bacillus macerans powder, 3 kilograms of Geobacillus stearothermophilus powder that above-described embodiment 1-5 prepares,
2 kilograms of 2 kilograms of bulkholderia cepasea powder, 2 kilograms of ditch Nocard's bacillus powder and excrement ghost umbrella powder, remix is used for described in uniform obtaining
The quick composting agent of livestock and poultry feces discarded object, total living bacteria count is 2.3 × 10 in decomposing agent8Individual/g.Fig. 1 is for animal dung
The quick composting agent process flow diagram of dirty discarded object.
Embodiment 7
Take 4 kilograms of Bacillus macerans powder, 3 kilograms of Geobacillus stearothermophilus powder that above-described embodiment 1-5 prepares,
2 kilograms of 3 kilograms of bulkholderia cepasea powder, 3 kilograms of ditch Nocard's bacillus powder and excrement ghost umbrella powder, remix is used for described in uniform obtaining
The quick composting agent of livestock and poultry feces discarded object, total living bacteria count is 2.2 × 10 in decomposing agent8Individual/g.
Embodiment 8
Take 2 kilograms of Bacillus macerans powder, 3 kilograms of Geobacillus stearothermophilus powder that above-described embodiment 1-5 prepares,
2 kilograms of 2 kilograms of bulkholderia cepasea powder, 3 kilograms of ditch Nocard's bacillus powder and excrement ghost umbrella powder, remix is used for described in uniform obtaining
The quick composting agent of livestock and poultry feces discarded object, total living bacteria count is 2.3 × 10 in decomposing agent8Individual/g.
Embodiment 9
Take 3 kilograms of Bacillus macerans powder, 2 kilograms of Geobacillus stearothermophilus powder that above-described embodiment 1-5 prepares,
2 kilograms of 2 kilograms of bulkholderia cepasea powder, 2 kilograms of ditch Nocard's bacillus powder and excrement ghost umbrella powder, remix is used for described in uniform obtaining
The quick composting agent of livestock and poultry feces discarded object, total living bacteria count is 1.9 × 10 in decomposing agent8Individual/g.
Embodiment 10
Take 3 kilograms of Bacillus macerans powder, 3 kilograms of Geobacillus stearothermophilus powder that above-described embodiment 1-5 prepares,
1 kilogram of 1 kilogram of bulkholderia cepasea powder, 2 kilograms of ditch Nocard's bacillus powder and excrement ghost umbrella powder, remix is used for described in uniform obtaining
The quick composting agent of livestock and poultry feces discarded object, total living bacteria count is 2.0 × 10 in decomposing agent8Individual/g.
Embodiment 11
Take 3 kilograms of Bacillus macerans powder, 3 kilograms of Geobacillus stearothermophilus powder that above-described embodiment 1-5 prepares,
2 kilograms of 2 kilograms of bulkholderia cepasea powder, 1 kilogram of ditch Nocard's bacillus powder and excrement ghost umbrella powder, remix is used for described in uniform obtaining
The quick composting agent of livestock and poultry feces discarded object.
Embodiment 12
Take 2 kilograms of Bacillus macerans powder, 2 kilograms of Geobacillus stearothermophilus powder that above-described embodiment 1-5 prepares,
3 kilograms of 1 kilogram of bulkholderia cepasea powder, 2 kilograms of ditch Nocard's bacillus powder and excrement ghost umbrella powder, remix is used for described in uniform obtaining
The quick composting agent of livestock and poultry feces discarded object.
Embodiment 13
Take 3 kilograms of Bacillus macerans powder, 2 kilograms of Geobacillus stearothermophilus powder that above-described embodiment 1-5 prepares,
1 kilogram of 1 kilogram of bulkholderia cepasea powder, 2 kilograms of ditch Nocard's bacillus powder and excrement ghost umbrella powder, remix is used for described in uniform obtaining
The quick composting agent of livestock and poultry feces discarded object.
Embodiment 14
Take 2 kilograms of Bacillus macerans powder, 3 kilograms of Geobacillus stearothermophilus powder that above-described embodiment 1-5 prepares,
2.5 kilograms of 1 kilogram of bulkholderia cepasea powder, 1 kilogram of ditch Nocard's bacillus powder and excrement ghost umbrella powder, remixes and uniformly obtain the use
In the quick composting agent of livestock and poultry feces discarded object.
Embodiment 15
Take 2 kilograms of Bacillus macerans powder, 2 kilograms of Geobacillus stearothermophilus powder that above-described embodiment 1-5 prepares,
1 kilogram of 1 kilogram of bulkholderia cepasea powder, 2 kilograms of ditch Nocard's bacillus powder and excrement ghost umbrella powder, remix is used for described in uniform obtaining
The quick composting agent of livestock and poultry feces discarded object.
Embodiment 16
Take 2.5 kilograms of Bacillus macerans powder, Geobacillus stearothermophilus powder 2.5 that above-described embodiment 1-5 is prepared
Kilogram, 1.5 kilograms of bulkholderia cepasea powder, 1.5 kilograms of ditch Nocard's bacillus powder and 2 kilograms of excrement ghost umbrella powder, remix uniform
To the quick composting agent for livestock and poultry feces discarded object.
Embodiment 17
Take 2 kilograms of Bacillus macerans powder, 3 kilograms of Geobacillus stearothermophilus powder that above-described embodiment 1-5 prepares,
3 kilograms of 1 kilogram of bulkholderia cepasea powder, 2 kilograms of ditch Nocard's bacillus powder and excrement ghost umbrella powder, remix is used for described in uniform obtaining
The quick composting agent of livestock and poultry feces discarded object.
Embodiment 18 is without Chinese medicine group
Take 2 kilograms of Bacillus macerans powder, 3 kilograms of Geobacillus stearothermophilus powder that above-described embodiment 1-5 prepares,
2 kilograms of 1 kilogram of bulkholderia cepasea powder and ditch Nocard's bacillus powder, remix the uniform corruption obtained for livestock and poultry feces discarded object
Ripe dose.
Embodiment 19 is without Bacillus macerans group
Take 3 kilograms of Geobacillus stearothermophilus powder, bulkholderia cepasea powder 1,000 that above-described embodiment 1-5 is prepared
Gram, 3 kilograms of 2 kilograms of ditch Nocard's bacillus powder and excrement ghost umbrella powder, remix the uniform decomposing agent obtained for livestock and poultry feces discarded object.
Embodiment 20 is without Geobacillus stearothermophilus group
Take 2 kilograms of Bacillus macerans powder, 1 kilogram of bulkholderia cepasea powder, ditch promise that above-described embodiment 1-5 is prepared
3 kilograms of 2 kilograms of Cattell bacterium powder and excrement ghost umbrella powder, remix the uniform decomposing agent obtained for livestock and poultry feces discarded object.
Embodiment 21 is without bulkholderia cepasea group
Take 2 kilograms of Bacillus macerans powder, 3 kilograms of Geobacillus stearothermophilus powder that above-described embodiment 1-5 prepares,
3 kilograms of 2 kilograms of ditch Nocard's bacillus powder and excrement ghost umbrella powder, remix the uniform decomposing agent obtained for livestock and poultry feces discarded object.
Embodiment 22 is without ditch Nocard's bacillus group
Take 2 kilograms of Bacillus macerans powder, 3 kilograms of Geobacillus stearothermophilus powder that above-described embodiment 1-5 prepares,
3 kilograms of 1 kilogram of bulkholderia cepasea powder and excrement ghost umbrella powder, remix and uniformly obtain becoming thoroughly decomposed for livestock and poultry feces discarded object
Agent.
The using effect checking of the decomposing agent of embodiment 23
In order to prove the using effect of quick composting agent of the invention, take obtained by above-described embodiment 6, embodiment 18-22
Decomposing agent verified, as a result as shown in table 1.
The decomposing agent using effect of table 1
Result shows：The dirty control group of decomposing agent fermentation animal excreta is not used and starts to decay the time for 15 days, using this skill
Decomposing agent fermentation animal excreta dirt prepared by art scheme starts to decay time advance 6 days, and become thoroughly decomposed time advance 12 days completely, N
Cellulose content is relative to be improved；And in embodiment 18-22, by using the agent prescription that becomes thoroughly decomposed to lack part compounding with respect to the technical program
Composition, although the control group that start to decay number of days and completely rotten number of days are relatively not used decomposing agent has shifted to an earlier date, and improves effect
Rate, but be not as good as the effect of the complete composition decomposing agent of the technical program, and fermentation ends carbon-nitrogen ratio nitrogen relative amount
It is higher.Excrement ghost umbrella can effectively facilitate four kinds of fermentations of bacterium.
Finally illustrate, preferred embodiment above is merely illustrative of the technical solution of the present invention and unrestricted, although logical
Cross above preferred embodiment to be described in detail the present invention, it is to be understood by those skilled in the art that can be
Various changes are made to it in form and in details, without departing from claims of the present invention limited range.
Claims (10)
1. a kind of quick composting agent for livestock and poultry feces discarded object, it is characterised in that the quick composting agent component is leaching fiber crops
Bacillus powder, Geobacillus stearothermophilus powder, bulkholderia cepasea powder, ditch Nocard's bacillus powder and excrement ghost umbrella powder；It is described
Total living bacteria count is 2~2.5 × 10 in decomposing agent8Individual/g.
2. quick composting agent as claimed in claim 1, it is characterised in that Bacillus macerans powder in the quick composting agent,
Geobacillus stearothermophilus powder, bulkholderia cepasea powder, ditch Nocard's bacillus powder and excrement ghost umbrella powder are 2-5 in mass ratio：2-
5：l-3：1-3：1-4.
3. quick composting agent as claimed in claim 1, it is characterised in that Bacillus macerans powder in the quick composting agent,
Geobacillus stearothermophilus powder, bulkholderia cepasea powder, ditch Nocard's bacillus powder and excrement ghost umbrella powder are 2 in mass ratio：3：2：
2：2.
4. the preparation method of a kind of quick composting agent for livestock and poultry feces discarded object described in any one of claims 1 to 3, it is special
Levy and be, its preparation method is following steps：
(1) Bacillus macerans powder is prepared：Bacillus macerans strain is inoculated in activation culture, Ran Houzhuan in slant medium
To enter be seeded to after Amplification Culture in eggplant-shape bottle fermentation cylinder for fermentation culture to spore forming rate and reach 90-95% to complete culture, hair
5~10 times of centrifugal concentrating zymotic fluid after ferment, then it is spray-dried to Bacillus macerans powder；
(2) Geobacillus stearothermophilus powder is prepared：Geobacillus stearothermophilus strain is inoculated in slant medium and is lived
Change culture, be then transferred to and be seeded to fermentation cylinder for fermentation culture to spore forming rate after Amplification Culture in eggplant-shape bottle and reach 90-
95% completes culture, 5~10 times of centrifugal concentrating zymotic fluid after fermentation, then spray-dried Geobacillus stearothermophilus powder；
(3) bulkholderia cepasea powder is prepared：Culture medium is inoculated into after bulkholderia cepasea conidia powder is mixed with flour to mix
On compound material, then koji tray cultivation and fermentation is carried out, through drying and crushing after fermentation completion, 100 mesh screen filtrations are obtained Burkholder
Salmonella powder；
(4) ditch Nocard's bacillus powder is prepared：It is inoculated on culture medium mixture material after ditch Nocard's bacillus conidia powder and flour are mixed,
Koji tray cultivation and fermentation is carried out again, and through drying and crushing after fermentation completion, 100 mesh screen filtrations are obtained ditch Nocard's bacillus powder；
(5) excrement ghost umbrella powder is prepared：Chinese medicine excrement ghost umbrella is crushed, 100 mesh screen filtrations；
(6) Bacillus macerans powder, Geobacillus stearothermophilus powder, bulkholderia cepasea powder, ditch promise Cattell are taken by formula
Bacterium powder and excrement ghost umbrella powder are well mixed, and obtain final product quick composting agent.
5. preparation method as claimed in claim 4, it is characterised in that the moisture content percentage by weight of the Bacillus macerans powder
Content is 5-6%；Bacillus macerans powder living bacteria count is 2~2.5 × 108Individual/g；Described is stearothermophilus ground bud
The moisture content weight percent content of spore bacillus powder is 5-6%；Geobacillus stearothermophilus powder living bacteria count for (2~2.5 ×
108Individual/g；The bulkholderia cepasea powder moisture content weight percent content is 5-6%；Bulkholderia cepasea powder is effectively lived
Bacterium number is 2~2.5 × 108Individual/g；The ditch Nocard's bacillus powder moisture content weight percent content is 5-6%；Ditch Nocard's bacillus powder
Living bacteria count is 2~2.5 × 108Individual/g.
6. preparation method as claimed in claim 4, it is characterised in that step (1) and step (2) described activation culture are 30-
37 DEG C are cultivated 24-40 hours；The fermented and cultured is to be cultivated 8-36 hours under the conditions of 30-32 DEG C；Culture medium is each in eggplant-shape bottle
Concentration of component is as follows：Tryptone 8-10g/L, yeast extract 3-5g/L, sodium chloride 3-5g/L, solvent is water；In fermentation tank
Culture medium each component concentration is as follows：Glucose 0.3-0.5g/L, beef extract 0.3-0.5g/L, peptone 0.3-0.5g/L and chlorination
Sodium 0.3-0.5g/L, solvent is water.
7. preparation method as claimed in claim 4, it is characterised in that spray drying process is to be sent out in contracting in step (1) and (2)
The precipitated calcium carbonate of concentrated broth weight 20%-40% is added in zymotic fluid and is stirred, passed through spray drying tower and entered
Row drying, intake air temperature is 195-200 DEG C, and air outlet temperature is 75-80 DEG C, the moisture content weight of the bacterium powder until keeping ejection
Percentage is between 5-6%；It is as follows specific method to be dried described in step (3) and (4)：By the conidia powder after fermentation in air inlet
Temperature is 195-200 DEG C, and air outlet temperature is dry at 75-80 DEG C.
8. preparation method as claimed in claim 4, it is characterised in that step (3) and step (4) the culture medium mixture material
To take wheat bran, bean cake powder and corn flour 60-70 in mass ratio:15-20:15-20 is mixed into material, adds quality of material 0.3-
0.5% epsom salt, the ferric sulfate of quality of material 2.5-3.5%, quality of material 0.1-0.2% potassium dihydrogen phosphates, and add
Entering water makes moisture be 50-60%；The koji tray cultivation and fermentation temperature is 25-30 DEG C, and fermentation time is 35-50 hours, hair
When ferment is finished conidia powder is covered with culture medium mixture material.
9. preparation method as claimed in claim 4, it is characterised in that step (3) and step (4) described conidia powder, flour and
The mass ratio of culture medium mixture material is 0.01-0.02:1-2:1-2.
10. the quick composting agent described in any one of claims 1 to 3 is processing answering during livestock and poultry feces discarded object produces organic fertilizer
With.
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Application publication date: 20170524 |