CN106688883A - Culture medium of double French marigold and preparation method of culture medium - Google Patents
Culture medium of double French marigold and preparation method of culture medium Download PDFInfo
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- CN106688883A CN106688883A CN201611011278.4A CN201611011278A CN106688883A CN 106688883 A CN106688883 A CN 106688883A CN 201611011278 A CN201611011278 A CN 201611011278A CN 106688883 A CN106688883 A CN 106688883A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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Abstract
The invention belongs to the technical field of plant tissue culture, and particularly relates to a culture medium of double French marigold and a preparation method of the culture medium. The culture medium is characterized by being prepared from, 5-15 parts of lard stearin, 5-10 parts of saccharose, 15-30 parts of activated carbon, 5-20 parts of pineapple peel, 30-35 parts of black soil, 2-6 parts of banana juice, 10-15 parts of fig leaves, 1-2 parts of spinach juice, 3-5 parts of rice water, 3-5 parts of soybean milk and 6-15 parts of fertilizer. By means of the culture medium, the problems that the double French marigold is low in division propagation coefficient and low in crossbreeding speed, and hybrid seeds are not prone to germination under the nature condition are solved, germination in vivo is achieved within 40 days and protocorm-like bodies are formed, differentiation multiple is up to 15, and the survival rate is 95% or above.
Description
Technical field
Invention belongs to the technical field of tissue culture of plant, and in particular to the culture medium of polyphyll maidenhair and its preparation side
Method.
Background technology
Maidenhair, alias yellow chrysanthemum flower, five valve lotuses, come always red, smelly chrysanthemum, tagetes patula, little marigold, reddish yellow grass, flannel flower,
It is the herb of feverfew maidenhair.Summer, autumn harvesting, using fresh herb or dry.It is born in patana, the woods of height above sea level 750-1600m
In, or in flower garden cultivation.It is distributed in the ground such as Sichuan, Guizhou, Yunnan.Maidenhair has medicinal and health-care effect.Floral leaf can be used as medicine,
There is clearing heat and eliminating phlegm, enrich blood and stimulate the menstrual flow.Pertussis, tracheitis, flu can be treated.Russian Caucasus region resident often to eat is used
Maidenhair, there is the effect promoted longevity.Maidenhair the sixth of the twelve Earthly Branches progressively turns into flower bed, the main body flowers of garden.The orange of it, yellow flower are extremely
It is eye-catching.Many anger are added to be planted part.Its effect and value have caused the concern of vast gardener author.
Maidenhair percentage of seedgermination is low, is only 10% -30% with common type of seeding germination percentage.Current plant peacock
Grass mode be：The container nurseries such as case, basin are first used, built-in sand loam creates soft, good permeability germination ring to seed
Border.Seed is directly broadcast on native table, overlying straw is covered with mat or newspaper, be allowed to be in close contact with soil.Conventional watering can waters, it is impossible to
Table soil is allowed to dry.After after seed sprouting, covering is thrown off.This traditional method germination percentage is relatively low, and the seedling for growing out is held
Easily die young.
The content of the invention
For above-mentioned deficiency, the invention provides the culture medium and preparation method thereof of polyphyll maidenhair, the present invention
Culture medium solve polyphyll maidenhair division propagation coefficient it is low, crossbreeding reproduction speed is slow, and hybrid seed is in natural bar
The problem for being difficult to sprout under part, meets the condition of multiple kind factorial praluctions.
To achieve these goals, the technical solution adopted by the present invention is as follows：
A kind of culture medium of polyphyll maidenhair, it is characterised in that be made up of the component of following weight portion：Lard stearin 5-15 parts, sugarcane
It is sugared 5-10 parts, activated carbon 15-30 parts, pineapple peel 5-20 parts, black earth 30-35 parts, bananas juice 2-6 parts, common fig leaf juice 10-15 parts,
Spinach juice 1-2 parts, 3-5 parts, soya-bean milk 3-5 parts, fertilizer 6-15 parts of rice water.
It is made up of the component of following weight portion as preferred, described fertilizer：Dandelion 8-10 parts, folium cortex eucommiae 8-10
Part, Ligusticum wallichii 5-8 part, Herba portulacae 8-15 parts, felwort 3-9 parts, Bark of Eucommia Ulmoides 16-26 parts, the old and the young year 19-27 part, soft-shelled turtle bone 1-2
Part, green tea 1-2 parts, each component process mixing, are then milled to 20-50 μm of powder and obtain final product.
Used as preferred, the preparation method of polyphyll maidenhair culture medium is comprised the following steps：
(1) aerobic fermentation after mixing lard stearin, meter Shui, sucrose, pineapple peel, fermentation pH control is in 7.0-8.0, fermentation
Time is 3-5 days, then carries out turning, and fermentation is stopped when fermentation temperature rises to 55-65 DEG C, is ground after tunning is dried
It is broken into 20-50 μm of powder；
(2) black earth, activated carbon, common fig leaf juice are mixed and is milled to 100-150 μm of powder, then airing is in the 5-7 that is exposed to the sun under sunlight
My god, add spinach juice, bananas juice, soya-bean milk and fertility preparation to be sufficiently mixed and obtain final product Nutrient medium after collecting powder.
In sum, as a result of such scheme, the present invention has advantages below to the present invention：
After planting sprout time is shorter for the seed that culture medium of the present invention is sprouted, and sprouts and forms protocorms in vivo within 40 days, point
Change multiple and reach as high as 15 plants, a bud can breed young plants up to a million in 1 year in theory；Culture medium of the present invention makes polyphyll peacock
The careless seedling speed of growth is accelerated to take root, and the young plant regularity produced is high；Prescription of rooting medium rooting rate was high, when inoculation 40 days
Afterwards, survival rate is more than 95%.
Specific embodiment
With reference to embodiment to culture medium of polyphyll maidenhair of the present invention and preparation method thereof, it is described further.
Embodiment 1：
A kind of culture medium of polyphyll maidenhair, is made up of the component of following weight portion：5 parts of lard stearin, 5 parts of sucrose, activated carbon 15
Part, 5 parts of pineapple peel, 30 parts of black earth, 2 parts of bananas juice, 10 parts of common fig leaf juice, 3 parts of 1 part, meter water of spinach juice, 3 parts of soya-bean milk, fertilizer 6
Part.
Described fertilizer is made up of the component of following weight portion：8 parts of dandelion, 8 parts of folium cortex eucommiae, 5 parts of Ligusticum wallichii, Herba portulacae 8
Then part, 3 parts of felwort, 16 parts of Bark of Eucommia Ulmoides, old and young 19 parts of year, 1 part of soft-shelled turtle bone, 1 part of green tea, each component grind by mixing
30 μm of powder are broken into obtain final product.
The culture medium preparation method of polyphyll maidenhair is comprised the following steps：
(1) aerobic fermentation after mixing lard stearin, meter Shui, sucrose, pineapple peel, fermentation pH is controlled in 7.0, fermentation time
It is 3 days, then carries out turning, fermentation is stopped when fermentation temperature rises to 55 DEG C, 30 μm is milled to after tunning is dried
Powder；
(2) black earth, activated carbon, common fig leaf juice are mixed and is milled to 120 μm of powder, then airing is collected in being exposed to the sun under sunlight 5 days
Spinach juice, bananas juice, soya-bean milk and fertility preparation is added to be sufficiently mixed and obtain final product Nutrient medium after powder.
Embodiment 2：
A kind of culture medium of polyphyll maidenhair, is made up of the component of following weight portion：10 parts of lard stearin, 8 parts of sucrose, activated carbon 20
Part, 10 parts of pineapple peel, 32 parts of black earth, 4 parts of bananas juice, 12 parts of common fig leaf juice, 4 parts of 1.5 part, meter water of spinach juice, 4 parts of soya-bean milk, fertilizer
10 parts of material.
Described fertilizer is made up of the component of following weight portion：9 parts of dandelion, 9 parts of folium cortex eucommiae, 6 parts of Ligusticum wallichii, Herba portulacae 10
Part, 6 parts of felwort, 20 parts of Bark of Eucommia Ulmoides, old and young 34 parts of year, 1.5 parts of soft-shelled turtle bone, 1.5 parts of green tea, each component pass through mixing, so
After be milled to 30 μm of powder and obtain final product.
The culture medium preparation method of polyphyll maidenhair is comprised the following steps：
(1) aerobic fermentation after mixing lard stearin, meter Shui, sucrose, pineapple peel, fermentation pH is controlled in 7.5, fermentation time
It is 4 days, then carries out turning, fermentation is stopped when fermentation temperature rises to 60 DEG C, 30 μm is milled to after tunning is dried
Powder；
(2) black earth, activated carbon, common fig leaf juice are mixed and is milled to 120 μm of powder, then airing is collected in being exposed to the sun under sunlight 6 days
Spinach juice, bananas juice, soya-bean milk and fertility preparation is added to be sufficiently mixed and obtain final product Nutrient medium after powder.
Embodiment 3：
A kind of culture medium of polyphyll maidenhair, is made up of the component of following weight portion：15 parts of lard stearin, 10 parts of sucrose, activated carbon
30 parts, 20 parts of pineapple peel, 35 parts of black earth, 6 parts of bananas juice, 15 parts of common fig leaf juice, 5 parts of 2 part, meter water of spinach juice, 5 parts of soya-bean milk, fertilizer
15 parts of material.
Described fertilizer is made up of the component of following weight portion：10 parts of dandelion, 10 parts of folium cortex eucommiae, 8 parts of Ligusticum wallichii, Herba portulacae
15 parts, 9 parts of felwort, 26 parts of Bark of Eucommia Ulmoides, old and young 27 parts of year, 2 parts of soft-shelled turtle bone, 2 parts of green tea, each component by mixing, then
30 μm of powder are milled to obtain final product.
The culture medium preparation method of polyphyll maidenhair is comprised the following steps：
(1) aerobic fermentation after mixing lard stearin, meter Shui, sucrose, pineapple peel, 8, fermentation time is for fermentation pH control
5 days, turning is then carried out, fermentation is stopped when fermentation temperature rises to 65 DEG C, 30 μm of powder are milled to after tunning is dried
End；
(2) black earth, activated carbon, common fig leaf juice are mixed and is milled to 120 μm of powder, then airing is collected in being exposed to the sun under sunlight 7 days
Spinach juice, bananas juice, soya-bean milk and fertility preparation is added to be sufficiently mixed and obtain final product Nutrient medium after powder.
The preferred embodiment of invention is the foregoing is only, is not intended to limit the invention, it is all in spirit of the invention
Within principle, any modification, equivalent substitution and improvements made etc. should be included within the scope of the present invention.
Claims (3)
1. a kind of culture medium of polyphyll maidenhair, it is characterised in that be made up of the component of following weight portion：Lard stearin 5-15 parts,
Sucrose 5-10 parts, activated carbon 15-30 parts, pineapple peel 5-20 parts, black earth 30-35 parts, bananas juice 2-6 parts, common fig leaf juice 10-15
Part, spinach juice 1-2 parts, 3-5 parts, soya-bean milk 3-5 parts, fertilizer 6-15 parts of rice water.
2. the culture medium of a kind of polyphyll maidenhair according to claim 1, it is characterised in that described fertilizer is by following heavy
The component for measuring part is made：Dandelion 8-10 parts, folium cortex eucommiae 8-10 parts, Ligusticum wallichii 5-8 parts, Herba portulacae 8-15 parts, felwort 3-9 parts,
Bark of Eucommia Ulmoides 16-26 parts, old and young year 19-27 part, 1-2 part of soft-shelled turtle bone, green tea 1-2 parts, each component through mixing, be then milled to
20-50 μm of powder is obtained final product.
3. the preparation method of the culture medium of a kind of polyphyll maidenhair according to claim 1 and 2, it is characterised in that prepare
Method is comprised the following steps：
(1) aerobic fermentation after mixing lard stearin, meter Shui, sucrose, pineapple peel, fermentation pH control is in 7.0-8.0, fermentation
Time is 3-5 days, then carries out turning, and fermentation is stopped when fermentation temperature rises to 55-65 DEG C, is ground after tunning is dried
It is broken into 20-50 μm of powder；
(2) black earth, activated carbon, common fig leaf juice are mixed and is milled to 100-150 μm of powder, then airing is in the 5-7 that is exposed to the sun under sunlight
My god, add spinach juice, bananas juice, soya-bean milk and fertility preparation to be sufficiently mixed and obtain final product Nutrient medium after collecting powder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611011278.4A CN106688883A (en) | 2016-11-17 | 2016-11-17 | Culture medium of double French marigold and preparation method of culture medium |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611011278.4A CN106688883A (en) | 2016-11-17 | 2016-11-17 | Culture medium of double French marigold and preparation method of culture medium |
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| CN106688883A true CN106688883A (en) | 2017-05-24 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201611011278.4A Pending CN106688883A (en) | 2016-11-17 | 2016-11-17 | Culture medium of double French marigold and preparation method of culture medium |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107372062A (en) * | 2017-07-25 | 2017-11-24 | 兴义市龙安园林绿化有限公司 | A kind of Bougainvillea spectabilis culture medium and preparation method thereof |
Citations (4)
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|---|---|---|---|---|
| CN102845311A (en) * | 2012-10-11 | 2013-01-02 | 山东鑫秋种业科技有限公司 | Method for preparing phalaenopsis plant tissue medium |
| CN104982185A (en) * | 2015-06-23 | 2015-10-21 | 柳州市天姿园艺有限公司 | French marigold planting method |
| CN105706883A (en) * | 2016-03-17 | 2016-06-29 | 陆开云 | Combined nutrition medium for planting Chinese flowering cabbage |
| CN105837310A (en) * | 2016-03-17 | 2016-08-10 | 陆开云 | Pollution-free nutrient medium for endive cultivation |
-
2016
- 2016-11-17 CN CN201611011278.4A patent/CN106688883A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102845311A (en) * | 2012-10-11 | 2013-01-02 | 山东鑫秋种业科技有限公司 | Method for preparing phalaenopsis plant tissue medium |
| CN104982185A (en) * | 2015-06-23 | 2015-10-21 | 柳州市天姿园艺有限公司 | French marigold planting method |
| CN105706883A (en) * | 2016-03-17 | 2016-06-29 | 陆开云 | Combined nutrition medium for planting Chinese flowering cabbage |
| CN105837310A (en) * | 2016-03-17 | 2016-08-10 | 陆开云 | Pollution-free nutrient medium for endive cultivation |
Non-Patent Citations (2)
| Title |
|---|
| 余琼芳等: "几种生物制药药渣对花卉生长发育的影响", 《西南农业学报》 * |
| 齐迎春等: "不同基因型孔雀草高效植株再生体系的建立", 《中国农业科学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107372062A (en) * | 2017-07-25 | 2017-11-24 | 兴义市龙安园林绿化有限公司 | A kind of Bougainvillea spectabilis culture medium and preparation method thereof |
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Application publication date: 20170524 |