CN106673877B - Agaricus bisporus casing material mainly prepared from chestnut calcium soil and preparation method thereof - Google Patents

Agaricus bisporus casing material mainly prepared from chestnut calcium soil and preparation method thereof Download PDF

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CN106673877B
CN106673877B CN201611250796.1A CN201611250796A CN106673877B CN 106673877 B CN106673877 B CN 106673877B CN 201611250796 A CN201611250796 A CN 201611250796A CN 106673877 B CN106673877 B CN 106673877B
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soil
vinegar residue
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agaricus bisporus
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CN106673877A (en
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郭尚
南晓洁
周林
刘秀珍
罗素兰
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Li Xia
Shanxi Institute Of Functional Food Shanxi Agricultural University
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Edible Fungi Research Institute Shanxi Academy Of Agricultural Sciences
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D9/00Other inorganic fertilisers
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/60Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants

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  • Chemical & Material Sciences (AREA)
  • Pest Control & Pesticides (AREA)
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Abstract

The invention discloses an agaricus bisporus casing material taking chestnut calcium soil as a main material, which comprises the following raw materials in parts by weight: 92-96 parts of chestnut calcium soil, 3-4 parts of bran coat,2-3 parts of vinegar residue. The preparation method of the casing material comprises the following steps: (1) collecting chestnut calcium soil below 20cm from ground surface, digging out, insolating for 2 days, and sieving with 3mm sieve; (2) soaking clean bran in 2% clear lime water for 24 hr, taking out, and draining; (3) crushing the clean vinegar residue, sieving the crushed vinegar residue with a 3mm sieve, soaking the crushed vinegar residue in 2% clear lime water for 24 hours, taking out the soaked vinegar residue, and draining the soaked vinegar residue for later use; (4) uniformly mixing the raw materials prepared in the steps (1), (2) and (3), adding water to regulate the moisture content to 18-22%, and using FeSO4Adjusting pH to 7.8-8.2, covering with plastic film, and sealing at room temperature for 20 days; (5) spraying 3% formaldehyde and 0.5% malathion with the spraying amount of 2% -3% of the weight of the soil covering material, and turning over the pile 3-4 times within two days to obtain the soil covering material of the agaricus bisporus.

Description

Agaricus bisporus casing material mainly prepared from chestnut calcium soil and preparation method thereof
Technical Field
The invention relates to the technical field of edible mushroom cultivation, in particular to an earth covering material for cultivating agaricus bisporus and a preparation method thereof.
Background
Agaricus bisporus (A)Agaricus bisporus) The edible fungus is a famous edible fungus with the widest cultivation area, the largest production scale and the largest yield in the world at present, has fat and tender meat quality, delicious and delicious taste, rich nutrition, high protein content, low fat content and high protein digestibility up to 88.5 percent, contains calcium, iron, zinc and other elements which are easy to be absorbed by human bodies, is rich in nutrient components such as various mineral substances, nucleotide, nicotinic acid, trace elements, vitamins and the like, also contains various amino acids and dozens of rare amino acids, has extremely high nutritional value, and is favored by people. The covering soil plays an important role in the cultivation process of the agaricus bisporus, the covering soil provides environmental change for the agaricus bisporus to grow so that the agaricus bisporus is changed from vegetative growth to reproductive growth, the specific physicochemical properties of the covering soil layer and the activity of microorganisms play an important role in the formation of agaricus bisporus sporocarp, the agaricus bisporus cultivation has the characteristic of no covering soil and no fruiting, and the covering soil is an essential link for stimulating the agaricus bisporus to form the sporocarp after mycelia grow to a certain stage. Meanwhile, the covering soil is also a main influence factor of the yield and quality of the agaricus bisporus and the uniformity of the commercial agaricus bisporus.
At present, the most ideal soil covering material is peat soil (also called peat soil), the maximum water holding capacity of the peat soil can reach 90 percent (the common soil is only 20 to 30 percent), the granular structure is good, the peat soil is loose and breathable, the peat soil is not hardened, the water spraying frequency is low in the growth and development process of young mushrooms, and the mushroom quality is good. The application of turfy soil in industrial cultivation in developed countries in the west is very common. The turfy soil is mainly produced in original forests in northeast China, although the turfy soil is widely applied to soil covering, the turfy soil is a non-renewable resource, and because a large amount of turfy soil is consumed in industrial production of agaricus bisporus every year, the turfy soil is expensive from purchasing and transportation to later-stage production. Therefore, under the current conditions of China, how to replace the simulated turfy soil with the local current resource agricultural wastes to achieve the purpose of improving the yield and the quality of the agaricus bisporus becomes a new trend for improving the cultivation means. If a method for imitating turfy soil with low price and convenient manufacture can be found, the method has great application and popularization values, and has important significance for reducing natural resource consumption and protecting the environment. Chinese patent 200810034603.8 discloses a preparation method using cow dung as an agaricus bisporus casing material, 201180055685.4 discloses a production method using bagasse as an agaricus bisporus casing material, 201410845464.2 discloses a production method using vermiculite as an agaricus bisporus casing material, and the like, all of which are agaricus bisporus casing materials developed by the inventors according to local conditions, and the above reports have positive significance for reducing the consumption of turfy soil resources in China, but there are some limitations due to different casing materials.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides the low-cost agaricus bisporus casing material which mainly comprises chestnut calcium soil and the preparation method thereof, and the invention is suitable for cultivating the agaricus bisporus in areas distributed with chestnut calcium soil.
The invention adopts the following technical scheme.
An agaricus bisporus casing material taking chestnut calcium soil as a main material comprises the following raw materials in parts by weight: 92-96 parts of chestnut calcium soil, 3-4 parts of bran coat and 2-3 parts of vinegar residue.
The invention relates to a preparation method of an agaricus bisporus casing material taking chestnut calcium soil as a main material, which comprises the following steps:
(1) preparing chestnut calcium soil raw materials, collecting chestnut calcium soil below 20cm from the ground surface, digging out, insolating for 2 days, and sieving with a 3mm sieve for later use;
(2) preparing bran raw materials, taking clean bran, soaking the clean bran in 2% clear lime water for 24 hours, taking out the clean bran and draining the clean bran for later use;
(3) preparing vinegar residue raw materials, crushing the clean vinegar residue, sieving the crushed vinegar residue with a 3mm sieve, soaking the crushed vinegar residue raw materials in 2% clear lime water for 24 hours, taking out the soaked vinegar residue raw materials, and draining the soaked vinegar residue raw materials for later use;
(4) uniformly mixing the raw materials prepared in the steps (1), (2) and (3), adding water to regulate the humidity to 18-22 percent of water content,
with FeSO4Adjusting pH to 7.8-8.2, covering with plastic film, and sealing at room temperature for 20 days;
(5) spraying 3% formaldehyde and 0.5% malathion with the spraying amount of 2% -3% of the weight of the soil covering material, and turning over the pile 3-4 times within two days to obtain the soil covering material of the agaricus bisporus.
The chestnut calcium soil used for preparing the agaricus bisporus casing soil develops under temperate semi-arid grassland vegetation. The method is mainly distributed in northwest areas and inner Mongolia autonomous regions in China. The surface layer is of chestnut color or dark chestnut color humus, the thickness is 25-45 cm, and the organic matter content is more than 1.5-4.0%; the calcium lamination layer containing a large amount of grey-white speck or powdery lime is below the humus layer, and the content of the lime reaches 10-30%. The humus content is less than that of black soil, but the soil is relatively fertile. The agaricus bisporus mycelium is loose in texture and good in permeability, and meets the basic requirements of agaricus bisporus mycelium growth.
The bran coat is a byproduct of millet processing from the millet. The bran coat has light volume weight and good air permeability, and can be added into chestnut calcium soil to improve the air permeability of chestnut calcium soil. Meanwhile, the bran coat contains triglyceride, free fatty acid, acipimic acid, lipoprotein, vitamin B, vitamin E, oryzanol, phytic acid, penta-carbonic acid, hexa-carbonic acid, glucose, cellulose and other substances. Vitamin B, citric acid and other nutrients may be absorbed and utilized for the growth and development of agaricus bisporus.
The vinegar residue is a byproduct of vinegar processing. Shanxi is a big province for vinegar brewing, and the vinegar residue by-product is large in amount and is not effectively utilized. The vinegar residue has loose texture and good permeability. The vinegar residue contains more crude protein and crude fiber, and is rich in microelements such as iron, zinc, selenium, manganese, etc., and can be absorbed and utilized for the growth and development of agaricus bisporus.
Therefore, in the prepared agaricus bisporus casing material, besides a large amount of chestnut calcium soil with almost no cost, a small amount of bran coat and vinegar residue not only has the function of increasing the air permeability of the casing material, but also has the function of providing nutrition for the growth of agaricus bisporus hyphae. Experiments prove that after the bran coat and the vinegar residue are added into the soil covering material, the indexes of non-capillary porosity, capillary water holding capacity and maximum water holding capacity also have synergistic effect.
The invention relates to an agaricus bisporus casing material taking chestnut calcium soil as a main material and a comparative test with a control turfy soil
1. Content of the experiment
The soil covering material test is provided with 5 treatments, which are respectively as follows: (1) peatmoss, (2) chestnut calcareous soil, (3) chestnut calcareous soil (96.5%) + bran coat (3.5%), (4) chestnut calcareous soil (97.5%) + vinegar residue (2.5%), (5) chestnut calcareous soil (94%) + bran coat (3.5%) + vinegar residue (2.5%), and the area of a cell is 1.5 × 1.4=2.1m2Each treatment was repeated 3 times; the content of the investigation includes: (1) the volume weight, non-capillary porosity, capillary water-holding capacity and maximum water-holding capacity indexes of different processed soil covering materials; (2) the dynamic change of the conductivity of the different treatment casing materials during the growth of the agaricus bisporus and the influence on the yield of the agaricus bisporus.
2. Test method
The culture material is cultured in 2016 year for 3 months and 24 days, the cultivation management is carried out for 18 days, the soil is covered in 4 months and 10 days after hypha grows over the material layer, the soil thickness is 3.5cm, the materials are raked up in 4 months and 13 days, the treatment is randomly arranged at the second layer of the mushroom house, the pesticide spraying and the watering are carried out according to the conventional management during the test period, the room temperature of the mushroom house is 21.2 ℃, the material temperature is 25.4 ℃, the humidity is 96.5 percent, and CO is carried out in the cultivation period2Concentration of 0.54%, cooling in 4 months and 18 days, room temperature of 17.7 deg.C, material temperature of 21.0 deg.C, humidity of 98%, and CO2The concentration is 0.15%, and the cultivation is carried out according to a conventional cultivation method. Observing growth condition and bud shape of mushroom mycelium in different covering soilYield of mature and mushroom. And respectively collecting covering soil analysis covering soil matrix EC25 at 0 day (before covering soil), 3 days, 6 days, 9 days (hypha growth period), 17 days (first fruiting time), 24 days (after first fruiting time) and 42 days (third fruiting time) of covering soil, and calculating the conductivity random distribution deviation coefficient C value at the spawn running period. And (4) collecting the first tide of mushrooms at 28 days to 6 days in 4 months in 2016, collecting the second tide of mushrooms at 9 days to 16 days in 5 months, collecting the third tide of mushrooms at 19 days to 26 days in 5 months, and recording the yield of each tide of mushrooms.
3. Test results
(1) Different treated soil covering capillary water holding capacity, maximum water holding capacity, non-capillary porosity and compacted volume weight
The water content of the sporophore of the agaricus bisporus is generally about 90 percent, and the condition that the agaricus bisporus can be used for providing more water is necessary for the growth and development of the agaricus bisporus. The water in the fruiting body is mainly from the casing material and the culture material, and the mushroom absorbs the water from the casing material to improve the water content of the fruiting body is very important, so that the mushroom has certain water holding capacity as the casing material. As can be seen from the data in Table 1, the indexes of non-capillary porosity, capillary water content and maximum water holding capacity of the peatmoss are the maximum in five treatments, further indicating that the peatmoss is the optimal agaricus bisporus casing material in terms of physical properties. Compared with the other four alternative covering materials, the test results are as follows: when the chestnut calcareous soil, the bran coat and the vinegar residue are used as the soil covering materials, the indexes of non-capillary porosity, capillary water content and maximum water holding capacity are all remarkably higher than those of other three processing materials; the indexes of the chestnut calcium soil and the bran coat, such as non-capillary porosity, capillary water content and maximum water holding capacity, are remarkably higher than those of the chestnut calcium soil, the vinegar residue and the pure chestnut calcium soil; the indexes of the non-capillary porosity, the capillary water content and the maximum water holding capacity of the chestnut calcium soil and the vinegar residue are remarkably higher than those of the pure chestnut calcium soil. Therefore, the covering material formed by combining the chestnut calcareous soil, the bran coat and the vinegar residue has an interactive synergistic effect on the influence of three indexes of non-capillary porosity, capillary water content and maximum water holding capacity. The test also shows that the porosity, capillary water holding capacity and maximum water holding capacity of the five treated non-capillary tubes can meet the basic water holding capacity required by the growth and development of the agaricus bisporus.
Figure 625073DEST_PATH_IMAGE001
(2) Dynamic variation of conductivity of different casing materials and influence on yield
The conductivity is an important parameter of soil fertility, and the fertilizer retention and supply characteristics, hydrodynamic characteristics and structural characteristics of the soil are closely related to the electrochemical characteristics of soil colloid. From the test results in the table 2, the average value of EC25 for treating the chestnut calcium soil, the millet bran and the vinegar residue is 1505 muS/cm, the average value of EC25 for treating the chestnut calcium soil and the vinegar residue is 1405 muS/cm, the average value of EC25 for treating the chestnut calcium soil and the vinegar residue is 1403 muS/cm, the average value of chestnut calcium soil and the vinegar residue is 1322 muS/cm, and the average value of peat soil is 1296 muS/cm, wherein the statistical analysis shows that the treatment of the chestnut calcium soil, the millet bran and the vinegar residue is remarkably higher than that of other four treatments; the difference between the treatment of the chestnut calcium soil and the treatment of the vinegar residue and the treatment of the chestnut calcium soil and the treatment of the bran coat is not obvious, but the difference between the treatment of the chestnut calcium soil and the treatment of the vinegar residue and the treatment of the chestnut calcium soil and the treatment of the bran coat are both obviously higher than that between the treatment of the chestnut calcium soil and the treatment; the chestnut calcium soil treatment is remarkably higher than the peatmoss treatment. The total yield of the mushrooms in the growing period corresponding to the five earthing treatments also shows a positive correlation, namely the higher the conductivity is, the higher the total yield is, and the total yield between the five treatments shows very obvious difference.
Figure 542213DEST_PATH_IMAGE002
Compared with the prior art, the invention has the advantages that: (1) the main raw material chestnut calcium soil of the agaricus bisporus casing material is easy to obtain and is particularly suitable for northern areas of China; (2) the auxiliary raw materials of vinegar residue and bran coat are main agricultural product processing byproducts in Shanxi province, resources are rich, meanwhile, the two raw materials also provide partial nutrient components for agaricus bisporus, and the two raw materials simultaneously use three indexes of non-capillary porosity, capillary water capacity and maximum water capacity of the soil covering material and have interaction synergism (3) tests prove that the soil covering material is increased by more than 20% compared with the traditional turfy soil; (4) the production and the manufacture are simple, large equipment investment is not needed, and the soil covering material has the greatest advantage of low production cost.
Example 1
The technical scheme of the invention is adopted to prepare the agaricus bisporus casing material.
(1) Preparing chestnut calcium soil raw materials, collecting chestnut calcium soil below 20cm from the ground surface, digging out, insolating for 2 days, and sieving with a 3mm sieve for later use;
(2) preparing bran raw materials, taking clean bran, soaking the clean bran in 2% clear lime water for 24 hours, taking out the clean bran and draining the clean bran for later use;
(3) preparing vinegar residue raw materials, crushing the clean vinegar residue, sieving the crushed vinegar residue with a 3mm sieve, soaking the crushed vinegar residue raw materials in 2% clear lime water for 24 hours, taking out the soaked vinegar residue raw materials, and draining the soaked vinegar residue raw materials for later use;
(4) weighing 92 kg of chestnut calcareous soil, 3 kg of bran coat and 2 kg of vinegar residue, uniformly mixing, adding water to regulate humidity to 18%, and then using FeSO4Adjusting pH to 7.8, covering with plastic film, and sealing at room temperature for 20 days;
(5) spraying 3% formaldehyde and 0.5% malathion with the spraying amount of 2% of the weight of the soil covering material, and turning over the pile 3-4 times within two days to obtain the soil covering material for the agaricus bisporus.
Example 2
The technical scheme of the invention is adopted to prepare the agaricus bisporus casing material.
(1) The preparation method of the chestnut calcareous soil, the bran coat and the vinegar residue is the same as that of the example 1;
(2) weighing 96 kg of chestnut calcareous soil, 4 kg of bran coat and 3 kg of vinegar residue, uniformly mixing, adding water to regulate the humidity to 22% and then using FeSO4Adjusting pH to 8.2, covering with plastic film, and sealing at room temperature for 20 days;
(5) spraying 3% formaldehyde and 0.5% malathion with the spraying amount of 3% of the weight of the soil covering material, and turning over the pile 3-4 times within two days to obtain the soil covering material for the agaricus bisporus.
Example 3
The technical scheme of the invention is adopted to prepare the agaricus bisporus casing material.
(1) The preparation method of the chestnut calcareous soil, the bran coat and the vinegar residue is the same as that of the example 1;
(2) weighing 94 kg of chestnut calcareous soil, 3.5 kg of bran coat and 2.5 kg of vinegar residue, uniformly mixing, adding water for humidifying until the water content is 20%, and then usingFeSO4Adjusting pH to 8.2, covering with plastic film, and sealing at room temperature for 20 days;
(5) spraying 3% formaldehyde and 0.5% malathion with the spraying amount of 2.5% of the weight of the soil covering material, and turning over the pile 3-4 times within two days to obtain the soil covering material of the agaricus bisporus.
Example 4
The technical scheme of the invention is adopted to prepare the agaricus bisporus casing material.
(1) The preparation method of the chestnut calcareous soil, the bran coat and the vinegar residue is the same as that of the example 1;
(2) weighing 92 kg of chestnut calcareous soil, 4 kg of bran coat and 2 kg of vinegar residue, uniformly mixing, adding water to regulate humidity to 20%, and then using FeSO4Adjusting pH to 8.1, covering with plastic film, and sealing at room temperature for 20 days;
(5) spraying 3% formaldehyde and 0.5% malathion with the spraying amount of 2.2% of the weight of the soil covering material, and turning over the pile 3-4 times within two days to obtain the soil covering material of the agaricus bisporus.
Example 5
The technical scheme of the invention is adopted to prepare the agaricus bisporus casing material.
(1) The preparation method of the chestnut calcareous soil, the bran coat and the vinegar residue is the same as that of the example 1;
(2) weighing 96 kg of chestnut calcareous soil, 3 kg of bran coat and 3 kg of vinegar residue, uniformly mixing, adding water to regulate humidity to 20%, and then using FeSO4Adjusting pH to 7.9, covering with plastic film, and sealing at room temperature for 20 days;
(5) spraying 3% formaldehyde and 0.5% malathion with the spraying amount of 2.8% of the weight of the soil covering material, and turning over the pile 3-4 times within two days to obtain the soil covering material of the agaricus bisporus.

Claims (2)

1. An agaricus bisporus casing material taking chestnut calcium soil as a main material comprises the following raw materials in parts by weight: 92-96 parts of chestnut calcium soil, 3-4 parts of bran coat and 2-3 parts of vinegar residue.
2. A method for preparing Agaricus bisporus casing material mainly containing chestnut calcium soil comprises the following steps:
(1) preparing chestnut calcium soil raw materials, collecting chestnut calcium soil below 20cm from the ground surface, digging out, insolating for 2 days, and sieving with a 3mm sieve for later use;
(2) preparing bran raw materials, taking clean bran, soaking the clean bran in 2% clear lime water for 24 hours, taking out the clean bran and draining the clean bran for later use;
(3) preparing vinegar residue raw materials, crushing the clean vinegar residue, sieving the crushed vinegar residue with a 3mm sieve, soaking the crushed vinegar residue raw materials in 2% clear lime water for 24 hours, taking out the soaked vinegar residue raw materials, and draining the soaked vinegar residue raw materials for later use;
(4) uniformly mixing the raw materials prepared in the steps (1), (2) and (3), adding water to regulate the humidity to 18-22 percent of water content,
with FeSO4Adjusting pH to 7.8-8.2, covering with plastic film, and sealing at room temperature for 20 days;
(5) spraying 3% formaldehyde and 0.5% malathion with the spraying amount of 2% -3% of the weight of the soil covering material, and turning over the pile 3-4 times within two days to obtain the soil covering material of the agaricus bisporus.
CN201611250796.1A 2016-12-30 2016-12-30 Agaricus bisporus casing material mainly prepared from chestnut calcium soil and preparation method thereof Active CN106673877B (en)

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