CN106665089A - Tissue re-culture replacement interplanting method in mint planting in saline-alkali soil - Google Patents

Tissue re-culture replacement interplanting method in mint planting in saline-alkali soil Download PDF

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Publication number
CN106665089A
CN106665089A CN201710000710.8A CN201710000710A CN106665089A CN 106665089 A CN106665089 A CN 106665089A CN 201710000710 A CN201710000710 A CN 201710000710A CN 106665089 A CN106665089 A CN 106665089A
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CN
China
Prior art keywords
herba menthae
saline
seedling
tissue culture
seedlings
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Pending
Application number
CN201710000710.8A
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Chinese (zh)
Inventor
魏清泉
杨长军
王海洋
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SHANDONG HUANGHE DELTA SUSTAINABLE DEVELOPMENT RESEARCH INSTITUTE
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SHANDONG HUANGHE DELTA SUSTAINABLE DEVELOPMENT RESEARCH INSTITUTE
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Priority to CN201710000710.8A priority Critical patent/CN106665089A/en
Publication of CN106665089A publication Critical patent/CN106665089A/en
Pending legal-status Critical Current

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Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for

Abstract

The invention discloses a tissue re-culture replacement interplanting method in mint planting in saline-alkali soil. The method is characterized in that in the first year, selected saline-alkali resistant peppermint seedlings are planted in the saline-alkali soil field with super-wide row spacing, in the first year, strong seedlings in a growth and development period are selected and second generation field mint seedlings are cultivated by a tissue culture and rapid propagation method. The seedlings are planted in current period and join the second season harvest. Then, by the same method and procedures, tissue culture replaces outdated-matured seedlings to interplant every two years. The mint growing in the field inherits and strengthens saline-alkali resistant gene and property of the mint all the time. The continuous high yield of the mint and gradual improvement of the soil are achieved. Compared with the prior art, the tissue re-culture replacement interplanting method in mint planting in the saline-alkali soil completely eradicates yield fluctuation of a one-time reseeding and trouble of new seeding import after the yield decreases year after year because of degeneration variation of the seedlings. The continuous high yield of the field planting and the gradual improvement of the soil are guaranteed and meanwhile, the seedlings inherit and strengthen the saline-alkali resistant gene and property.

Description

Again tissue culture substitutes interplanting method in the Herba Menthae plantation of salt-soda soil
First, technical field
The invention belongs to agricultural planting biotechnology, again tissue culture is substituted in specifically a kind of salt-soda soil Herba Menthae plantation Interplanting method.
2nd, background technology
Wetland with saline-alkaline and saline Land are the important problems for perplexing agricultural production, how using salt-soda soil and prevention soil Ground salinization of soil, is the professional and technical personnel's important topic for facing and object of tackling key problems.Study for many years and test and find Mentha piperita tool There is Salt And Alkali Tolerance and reduce the critical function of soil saline and alkaline, while with very high economic worth.But, in salt-soda soil green pepper is planted The process of sample Herba Menthae and empirical discovery:In the production process of salt-soda soil, saline and alkaline tolerance declines Mentha piperita, is finally difficult to keep away Exempt from withered death final result.The Basic practice of prior art is the overall change of crops, and initial seedling is planted again.
3rd, the content of the invention
Again tissue culture substitutes interplanting method in a kind of salt-soda soil Herba Menthae plantation, it is characterized in that in salt-soda soil field planting Herba Menthae First Year, ultra-wide line-spacing plantation choose salt resistance alkali Herba Menthae seedling.Period of development strong sprout is chosen after emerging, using tissue-culturing rapid propagation side Method, cultivates the lineal secondary seedling of land for growing field crops Herba Menthae, interplants in big ridge Herba Menthae land for growing field crops in current, and the Herba Menthae of secondary seedling participates in regrowth hair Harvest, the symbiosis of two generations and output are realized then.
Again tissue culture substitutes interplanting method in a kind of described salt-soda soil Herba Menthae plantation, it is characterized in that next year in land for growing field crops Herba Menthae After emerging, the strong sprout tissue culture three generations land for growing field crops Herba Menthae seedling in secondary Herba Menthae Seedling is chosen, after the first planting is harvested, interplant again or replace Generation Lao Miao.Realize land for growing field crops three generations's Herba Menthae symbiosis, second and third generation symbiosis.
Again tissue culture substitutes interplanting method in a kind of described salt-soda soil Herba Menthae plantation, it is characterized in that in per generation Herba Menthae salt resistance Alkalescence can occur before degeneration variation, at once substituted interplanting of new generation, and method described above and the continuous tissue culture of program are more of new generation The new seed of salt resistance alkali Herba Menthae.
Again tissue culture substitutes interplanting method in a kind of described salt-soda soil Herba Menthae plantation, it is characterized in that above-mentioned replacement Herba Menthae kind The cultivating process of Seedling, is choosing and is carrying out in field soil substrate identical experimental plot, to keep Herba Menthae seedling of new generation One's best quality pure between generation.
Again tissue culture substitutes interplanting method compared with prior art in the salt-soda soil Herba Menthae plantation of the present invention, with following aobvious Work feature and advantage:
1. Mentha piperita can germinate and healthy and strong development in the soil of high salinity quality, decline in production cycle and the change of crops Lose and dead.Confirm that Mentha piperita has the saline and alkaline gene of opposing.Can resist and fit in the life animated period of its germinating growth Answer saline and alkaline erosion damage.And the duration and period of decline in growth, then it is difficult to resist saline and alkaline lasting infringement and accumulation is injured, And there is degeneration variation, lose salt resistance alkali gene and respective capabilities.This method selection-breeding has salt resistance alkali gene and in saline and alkaline ring Further the strong sprout of reinforcing, as seed cultivation seedling of lower generation, passes on and enhances the saline-alkali tolerance energy of Mentha piperita in border.It is raw Prevent the underproduction season by season caused because of variation of degrading during product, form the benign cycle even increased production season by season without season difference.
2. in production land for growing field crops or breeding experiment Tian Xuanmiao tissue cultures, process is simple and easy to control, and sequencing is strong, with low content of technology, into Fruit is reliable, and technology is easy to excavate and promotes.
3. updated using the substituting interplanting of interplanting and part, it is to avoid large stretch of, large area updates the periodicity that multiple cropping brings Personnel's busy, seedling be full of it is scarce, yield Feng Qian etc. change and fluctuate.Stable and sustainable development to enterprise staff, technology, yield is carried Supply to ensure.
4. constantly update regenerate, persistently and stronger salt resistance alkali Herba Menthae production process be more conducive to soil salinity reduction and The improvement of soil.
4th, specific embodiment
With reference in salt-soda soil Herba Menthae plantation of the embodiment to the present invention, again tissue culture substitutes interplanting method work further Describe in detail:
The present invention is embodied as in Saline-alkaline Soils of Yellow River Delta production land for growing field crops, and farm soil top layer arm's length basis is measured in advance Salinity changes obvious in 0.4-0.6% with season and drought and waterlogging situation salinity.In spring in the whole land for growing field crops trained, with ridge width 50cm, spacing in the rows 8-10cm plants the Mentha piperita of selection-breeding tissue culture.Emergence rate 90%, growth course is good.The July first planting is gathered in, Average 450kg/ mus of harvest yield or so, the harvesting of October regrowth hair, the average 250kg/ mus of harvest yield or so.Winter management is focused on protecting Moisture in the soil.Spring in the coming year chooses strong sprout after emerging, and using tissue culture and rapid propagation method, to cultivate and interplant land for growing field crops, secondary seedling after the secondary seedling in land for growing field crops Emergence rate 98%.Participate in regrowth hair then to harvest, the average 450kg/ mus of harvest yield.In secondary Seedling development working days selection-breeding strong sprout tissue culture the Three generations land for growing field crops seedling, replaces generation Lao Miao to interplant in land for growing field crops on the 3rd year.Emergence rate up to 100%, the average 800kg/ of one batch of harvest yield Mu, the average 500kg/ mus of regrowth hair harvest yield.Next year repeat procedure above later, Lao Miao interplantings a new generation is substituted in process of production Land for growing field crops seedling, realizes continuous high yield.

Claims (4)

1. again tissue culture substitutes interplanting method in a kind of salt-soda soil Herba Menthae plantation, it is characterized in that in salt-soda soil field planting Herba Menthae First Year, the salt resistance alkali Herba Menthae seedling that the plantation of ultra-wide line-spacing is chosen chooses period of development strong sprout, using tissue-culturing rapid propagation side after emerging Method, cultivates the lineal secondary seedling of land for growing field crops Herba Menthae, interplants in big ridge Herba Menthae land for growing field crops in current, and the Herba Menthae of secondary seedling participates in regrowth hair Harvest, the symbiosis of two generations and output are realized then.
2. again tissue culture substitutes interplanting method in a kind of salt-soda soil Herba Menthae plantation according to claim 1, it is characterized in that every Year after land for growing field crops Herba Menthae emerges, chooses the strong sprout tissue culture three generations land for growing field crops Herba Menthae seedling in secondary Herba Menthae Seedling, after the first planting is harvested, then Secondary interplanting replaces generation Lao Miao.Realize land for growing field crops three generations Herba Menthae symbiosis or second and third generation symbiosis.
3. again tissue culture substitutes interplanting method in a kind of salt-soda soil Herba Menthae plantation according to claim 1 and 2, it is characterized in that Before per generation Herba Menthae saline-alkali tolerance can occur to degrade and make a variation, at once substituted of new generation is interplanted, and method described above and program connect The new seed of continuous tissue culture salt resistance alkali Herba Menthae more of new generation.
4. again tissue culture substitutes interplanting method, its feature in a kind of salt-soda soil Herba Menthae plantation according to claim 1,2 and 3 It is the cultivating process of above-mentioned replacement Herba Menthae seedling, is choosing and carrying out in field soil substrate identical experimental plot, to keep One's best quality pure between the generation of Herba Menthae seedling of new generation.
CN201710000710.8A 2017-01-03 2017-01-03 Tissue re-culture replacement interplanting method in mint planting in saline-alkali soil Pending CN106665089A (en)

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Application Number Priority Date Filing Date Title
CN201710000710.8A CN106665089A (en) 2017-01-03 2017-01-03 Tissue re-culture replacement interplanting method in mint planting in saline-alkali soil

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Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102630564A (en) * 2012-03-29 2012-08-15 山东省科学院中日友好生物技术研究中心 Tissue culture and rapid propagation method of salt-tolerant field mint

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102630564A (en) * 2012-03-29 2012-08-15 山东省科学院中日友好生物技术研究中心 Tissue culture and rapid propagation method of salt-tolerant field mint

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
孟祥卫: "盐碱地薄荷的丰产栽培技术", 《农业科技通讯》 *
童启庆主编: "《茶树栽培学 第3版》", 30 June 2007, 中国农业出版社 *
贾晓东等: "3种药赏两用植物对滨海盐碱土改良效应的比较", 《植物资源与环境学报》 *
赵国芳等: "薄荷的退化混杂与防止", 《农业科技通讯》 *
陈连官: "薄荷留兰香品种耐盐特性研究", 《上海农业科技》 *
黄龙斌等: "椒样薄荷收获加工后生长势衰弱的原因及预防措施", 《新疆农垦科技》 *

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Inventor after: Wang Haiyang

Inventor after: Wei Qingquan

Inventor after: Yang Changjun

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