CN106631446A - Organic master batch, and preparation method and application thereof - Google Patents
Organic master batch, and preparation method and application thereof Download PDFInfo
- Publication number
- CN106631446A CN106631446A CN201611178333.9A CN201611178333A CN106631446A CN 106631446 A CN106631446 A CN 106631446A CN 201611178333 A CN201611178333 A CN 201611178333A CN 106631446 A CN106631446 A CN 106631446A
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- master batch
- polyglutamic acid
- organic
- mass percent
- organic master
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/10—Solid or semi-solid fertilisers, e.g. powders
- C05G5/12—Granules or flakes
Abstract
The invention discloses an organic master batch. The organic master batch contains 500-600 mg/kg of polyglutamic acid, has a living bacterium count of 0.2-0.9 billion/g, and comprises 0-8% of nutrient in percentage by mass and 40-60% of organic matter in percentage by mass. The invention also discloses a preparation method and application of the organic master batch. Compared with the prior art, the product disclosed by the invention is low in water content and high in particle hardness, can be singly applied or mixed with a composite fertilizer, and has favorable effects in the aspects of preventing premature senescence of crops, realizing early harvesting of the crops and improving quality of the crops. Besides, the product has the effects of moisture preservation, fertilizer preservation and soil moisture preservation. Meanwhile, the preparation method of the product is simple and easy to popularize.
Description
Technical field
The invention belongs to agriculture field, and in particular to a kind of organic master batch and preparation method and application.
Background technology
Due to long-term irrational farming and irrational irrigation, a large amount of administrations of chemical fertilizer, soil aggregate is exacerbated
Destruction, cause soil compaction increasingly severe, directly influence the natural vigor and capacity of self-regulation of soil.It is excessively used
Chemical fertilizer, agricultural chemicals, the residual contamination of agricultural film, undressed fertilizer pollution, and continuous cropping and the pollution of pest and disease damage pathogen, once
" will overflow " after soil self-purification ability.This can cause soil ecology unbalance, make soil beneficial organism and beneficial micro- life
Thing mortality, geobiont population is reduced, and soil physical chemistry biological property deteriorates, and soil activation declines, soil function is deteriorated, and loses
Agricultural use is gone to be worth.Also corresponding some germs is bred year after year, accumulate in a large number in soil, also preceding crop root
The secretion of system forms sick soil because of build up as noxious material.
Containing the effective active composition such as lignin, humic acid and polyglutamic acid and withered in the organic master batch of gamma-polyglutamic acid
The microbial inoculum of this kind of raising soil disease resistance of careless bacillus.Recently polyglutamic acid, withered grass active microbial inoculum and lignin are in agricultural
Application in production is more, and it is mainly manifested in the following aspects：
1) polyglutamic acid is a kind of macromolecule amino acid polymer, and chemical constitution is special, can effectively prevent waving for nitrogenous fertilizer
Loss, the fixation of potash fertilizer are sent out, prevents phosphate radical, sulfate radical and metallic element from producing precipitation, trace element is improved in chelating
Crop rhizosphere soils nutrient density.Because it has good water retention property, liquid manure good around crop rhizosphere can be built bad
Border, improves the absorbability of crop nutrition content, makes absorption of the crop to nutrient have fast and efficiently characteristic.Crop is stimulated to produce
Endogenous hormone, stimulates crop root development, improves photosynthesis, improves crop anti-adversity energy.
2) bacillus subtilis is Gram-positive bacillus, can produce endogenous spore, heat-resisting strong stress resistance, in soil
With the surface generally existing of plant.It is a kind of endophyte common in plant body, nontoxic to people and animals, free from environmental pollution.
Simple production process, preparation stabilization applies convenient, and Storage period is long.Bacillusmusilaginosiengineering is also referred to as silicate bacteria, the bacterium tool
Have the function of phosphorus decomposing, potassium decomposing, can silicon, calcium, the effect of magnesium moderate-element in activating soil, improve or extend fertilizer efficiency, reduce chemical fertilizer
Consumption, and crop anti-adversity is improved, prevent or mitigate disease.Bacillus subtilis and bacillusmusilaginosiengineering are widely used in life
Thing fertilizer.When crop or soil is acted on. fast growth, nutritional need are simple, are easy to survive, colonize on the surface of plant
With breeding.
3) in the scope of agricultural application also than wide, it has spy that is nontoxic, inexpensive, being decomposed by the microorganisms to lignin
Property.Prolongation fertilizer efficiency time can be played when using cooperatively with the element such as Nitrogen, phosphorus, potassium, the effect of fertilizer loss is reduced.
Lignin is alternatively arranged as soil conditioner, for the hardened serious, salinization soil of improved soil, makes soil produce crumb structure,
And then change soil moisture characteristic.Lignin also with sequestering, can effectively prevent phosphate fertilizer to be fixed on soil colloid, significantly
Improve fertilizer efficiency.
To sum up, using polyglutamic acid, active microbial inoculum, npk nutrient, lignin and concentration liquid molasses ammoniation-granulation
Organic master batch will have huge application prospect.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of organic master batch, to solve the effect on driving birds is not good of prior art presence
The problems such as.
The technical problem also to be solved of the invention is to provide the preparation method of above-mentioned organic master batch.
The technical problem finally to be solved of the invention is to provide the application of above-mentioned organic master batch.
To solve above-mentioned technical problem, the technical solution used in the present invention is as follows：
A kind of organic master batch, it includes the polyglutamic acid of 500mg/kg～600mg/kg, effective work of 200,000,000/g～900,000,000/g
Bacterium number, the organic matter that mass percent is 0～8% nutrient and mass percent is 40～60%.
Wherein, described polyglutamic acid be polyglutamic acid sterling, polyglutamate sterling, comprising polyglutamic acid fermentation training
Any one or a few combination in foster thing, polyglutamic acid solution and the wettable powder containing polyglutamic acid zymotic fluid.
Wherein, the molecular weight of described polyglutamic acid is 1～3000kDa.
Wherein, described effective viable bacteria is bacillus subtilis and/or bacillusmusilaginosiengineering.
Wherein, described Source Organic Matter is lignin and concentration molasses bacterium solution.
Wherein, described nutrient is N, P2O5And K2O。
Wherein, N is from ammonium sulfate and MAP, P2O5From MAP, K2O is from concentration molasses bacterium solution.
Wherein, described lignin is prepared by complete water-soluble lignin Jing after 50～58 DEG C of 7～10d that become thoroughly decomposed.
Wherein, described concentration molasses bacterium solution is prepared by the following method：
By bacillus subtilis and/or bacillusmusilaginosiengineering Jing after seed culture medium culture, with the ratio of 10～20%v/v
Example accesses fermented and cultured in fermentation medium so that living bacteria count reaches 100～30,000,000,000/g in zymotic fluid；By cane molasses
After being configured to the molasses aqueous solution of mass percent 50～60%, add molasses water-soluble with 10～20% volume ratio zymotic fluid
In liquid, 30～40 DEG C of bottom fermentations are obtained final product for 2～3 days.
Wherein, the formula of described fermentation medium be sucrose 2.5～3%, cornstarch 3.5～4%, potassium dihydrogen phosphate
1-1～5%, dusty yeast 0.5～0.6%, magnesium sulfate 0.05～0.06%, water 91%～92.5%, incubation time is 72h, training
Foster temperature is 36～37 DEG C.
The preparation method of above-mentioned organic master batch, it comprises the steps：
By granulating of reuniting in ammonium sulfate, MAP, concentration molasses bacterium solution, lignin and polyglutamic acid input comminutor,
The wet stock of the granulating for obtaining is obtained final product Jing after hardening by cooling, drying and sieving.
Wherein, in organic master batch, the mass percent of ammonium sulfate is 15～20%, and the mass percent of MAP is 3
～8%, the mass percent for concentrating molasses bacterium solution is 15～20%, and the mass percent of lignin is 50～65%, polyglutamic
The mass percent of acid is 0.05～-0.06%.
Wherein, in comminutor, reaction temperature is 70～80 DEG C.
Application of the above-mentioned organic master batch in crop yield is improved is also within protection scope of the present invention.
Wherein, application process is, by organic master batch and composite fertilizer with 1:Use after 3～4 mass ratio mixing.
Beneficial effect：
Compared with prior art, the present invention has following advantage：
1st, product water content of the present invention is low, and pellet hardness is high, can be administered alone or apply with the blending such as urea, composite fertilizer or
Apply with the blending such as urea, composite fertilizer or to apply shelf life with the blending such as urea, composite fertilizer long, be not result in that fertiliser granulates collapse
Solution.It is the bulk blended fertilizer particle of raw material substitution about 20～25%/nutrient (10kg/40kg～12.5kg/ when blending with composite fertilizer
37.5kg this master batch blending application effects, can be better than 50kg common fertilizer application effects).
2nd, the peculiar addition highly-concentrated polyglutamic acid of this master batch and active gemma flora, can significantly improved soil nodule structure and
Physicochemical structure property, reduces hardened, dissolving phosphor and dissolving potassium, increases edaphon and retentiveness；And resist with crop is dramatically increased
Drought, cold-resistant, resistant to lodging, salt resistance alkali ability, and resist continuous cropping there is certain effect.
3rd, the active factors polyglutamic acid that this product contains stimulates crop to produce endogenous hormones, to preventing crop early ageing, doing sth. in advance
Crop collecting time, lifting crop quality (fruit sugariness, storage stability, increase Vc contents etc.) have certain effect.
4th, the product is applied and have in soil good soil moisture conservation effect, and retain water and nutrients, lignin is slowly resolved into corruption
Grow acid, improved soil and do not produce any harmful substance, environmental protection.
Specific embodiment
According to following embodiments, the present invention may be better understood.The raw materials used source of following examples is as follows：
Gamma-polyglutamic acid：Nanjing Shinekingbiotech, Ltd. provides.
Bacillus subtilis or bacillusmusilaginosiengineering are separated Jing after culture, then 10% ratio is linked into fermentation medium
In 36～37 DEG C of bottom fermentation culture 3d, the bacillus subtilis suspension or bacillusmusilaginosiengineering will be added in molasses fermented liquid
Suspension 20% simultaneously carries out continuation fermentation, and MgSO is added in culture40.5g/L, MnSO4·H2O 0.5g/L, improve gemma ratio
For more than 80%, fermentation temperature is 30～40 DEG C, is fermented 2-3 days.
Lignin main source is papermaking black liquor lignin, is become thoroughly decomposed fermentation, and above-mentioned polyglutamic acid, withered grass gemma by high temperature
The granulation of the same lignin of bacillus molasses concentrated broth, ammonium sulfate and MAP, obtains the organic master batch of polyglutamic acid.
Embodiment 1：
One kind granulates master batch based on the gamma-polyglutamic acid and high temperature lignin that becomes thoroughly decomposed with the molasses fermented bacterium solution of concentration, its formula
For polyglutamic acid fermenation raw liquid 1.5%, molasses fermented bacterium solution 15%, ammonium sulfate 15.5%, MAP 3%, lignin are concentrated
65%.Wherein 33～the 40g/L of content containing polyglutamic acid in polyglutamic acid stoste, bacteria used thereby is withered grass gemma in concentrated fermenting liquid
Bacillus, viable count reaches 4.0*10 after fermentation9cfu/g.The fertilizer items component target for obtaining is detected as polyglutamic acid 550mg/
kg；Organic matter 59%；N+P2O5+K2O is 5.04%；PH value 6.7；Moisture 8.4%；Living bacteria count (cfu) hundred million/g：5.6；Excrement
Coliform count, individual/g：78；The roundworm death rate：98.3%.
The concrete preparation method of above-mentioned organic master batch includes following step：
1) culture of bacillus subtilis, after cultivating in seeding tank, is linked into fermentation medium according to 10% ratio
80% molasses fermented liquid after fermented and cultured, then 20% ratio addition steam sterilizing carries out secondary fermentation, adds in culture
MgSO40.5g/L, MnSO4·H2O 0.5g/L, it is more than 80% to improve gemma ratio, and fermentation temperature is 37 DEG C, and ferment 2-3
My god.
2) lignin is proportionally added into polyglutamic acid stoste, ammonium sulfate, MAP, wood after 58 DEG C of high temperature nuclear reactors are rotten 3 weeks
Quality and the molasses fermented bacterium solution of concentration, are entered in comminutor by electronic measurement Belt Conveying.Made with unclassified stores at 70-80 DEG C
Grain, the wet stock of granulating enters back into drier dehydration after hardening by cooling on belt conveyer.Material after drying is by carrying
The machine of liter is transported to screening machine, and the bulky grain after screening returns comminutor and granulates again together with the lower fines of sieve after crushing.
By taking the tomato that grows fruit as an example, the great ecological park in Nanjing Pu is experimental field arranged on, tomato variety is Nanjing beautiful jade, is transplanted
Time is in March, 2015, and ripe about mid-May, sustained yield is to July.This test sets 2 process, three repetitions, cell face
Product 100m2, each cell random alignment, setting protection row.Conventional fertilizer application (COM) is composite fertilizer 50kg, using (OPT) generation of the invention
After in composite fertilizer 20% nutrient, i.e. 40kg composite fertilizers+10kg fertilizers, other fertilizer treatment all sames, concrete data are shown in
Following table.
Table 1 respectively processes the impact to tomato yield
By contrast, tomato fruit is harvested 6 days ahead of time by the more conventional fertilizer treatment of present invention fertilising, extends collecting time
About 13 days, yield increased by 18.46%.
Embodiment 2：
Substantially the same manner as Example 1, except that being added thereto, lignin, MAP ratio are different, it is specifically matched somebody with somebody
Side is polyglutamic acid stoste 1.5%；Concentrate molasses fermented bacterium solution 15%；Ammonium sulfate 16.5%；MAP 8%, lignin
59%.Wherein 33～the 40g/L of content containing polyglutamic acid in polyglutamic acid stoste, bacteria used thereby is withered grass gemma in concentrated fermenting liquid
Bacillus.The fertilizer items component target for obtaining is detected as polyglutamic acid 590mg/kg；Organic matter 52%；N+P2O5+K2O is
7.9%；PH value 6.3；Moisture 7.5%；Living bacteria count (cfu) hundred million/g：4.7；Colibacillus of excrement number, individual/g：64；Roundworm is dead
Rate：98.6%.
As a example by plant Chinese cabbage, being experimental field arranged in the Pan Cheng warmhouse booths of Nanjing is carried out, and varieties of Brassica campestris is that short-foot is yellow,
Transplanting time is in January, 2015, and this test sets 2 process, three repetitions, plot area 30m2, each cell random alignment, setting
Protection row.Conventional fertilizer application (COM) is composite fertilizer 50kg, and using (OPT) of the invention in composite fertilizer 20% nutrient, i.e. 40kg are replaced
Composite fertilizer+10kg, other fertilizer treatment all sames, concrete data see the table below.
Table 2 respectively processes the impact to yield of pakchoi
By contrast, pakchoi improves 5.9cm by the more conventional fertilizer treatment plant height of present invention fertilising, and chlorophyll improves
0.6mg/L, yield increases by 12.9%.
Embodiment 3：
Substantially the same manner as Example 1, except that the bacterial classification being added thereto is different, its Ju Ti Pei Fang is that polyglutamic acid is former
Liquid 1.5%；Concentrate molasses fermented bacterium solution 15%；Ammonium sulfate 15%；MAP 3%；Lignin 65%.Wherein polyglutamic acid is sent out
33～the 40g/L of content containing polyglutamic acid in ferment stoste, bacteria used thereby is bacillusmusilaginosiengineering in concentrated fermenting liquid.What is obtained has
Machine fertilizer items component target is detected as polyglutamic acid 550mg/kg；Organic matter 59%；N+P2O5+K2O is 5.04%；PH value 6.7；
Moisture 8.4%；Living bacteria count (cfu) hundred million/g：2.1；Colibacillus of excrement number, individual/g：75；The roundworm death rate：98.7%.
As a example by plant cucumber, the great ecological park in Nanjing Pu is experimental field arranged on, cucumber variety is southern water 2, transplanting time
For in March, 2015, it is ripe about by the end of April, sustained yield is to June.This test sets 2 process, three repetitions, plot area
100m2, each cell random alignment, setting protection row.Conventional fertilizer application (COM) is composite fertilizer 100kg, using (OPT) generation of the invention
After in composite fertilizer 20% nutrient, i.e. 80kg composite fertilizers+20kg fertilizers, other fertilizer treatment all sames, concrete data are shown in
Following table.
Table 3 respectively processes the impact to cucumber yield and quality
Apply the invention product and compare conventional composite fertilizer 100kg for per mu and compare, yield increases by 14.16%, and cucumber is adopted ahead of time
Receive 8 days, Vitamin C content improves 30%, and quality is high.
Embodiment 4
It is substantially the same manner as Example 1, except that the mass percent of organic matter is 40%.
Claims (12)
1. a kind of organic master batch, it is characterised in that it includes the polyglutamic acid of 500mg/kg～600mg/kg, 200,000,000/g～7.5
The living bacteria count of hundred million/g, the organic matter that mass percent is 0～8% nutrient and mass percent is 40～60%.
2. organic master batch according to claim 1, it is characterised in that described polyglutamic acid is polyglutamic acid sterling, poly-
Glutamate sterling, the fermentation culture medium comprising polyglutamic acid, polyglutamic acid solution and containing the wettable of polyglutamic acid zymotic fluid
Any one or a few combination in property pulvis.
3. organic master batch according to claim 1, it is characterised in that the molecular weight of described polyglutamic acid is 1～
3000kDa。
4. organic master batch according to claim 1, it is characterised in that described effective viable bacteria be bacillus subtilis and/
Or bacillusmusilaginosiengineering.
5. organic master batch according to claim 1, it is characterised in that described Source Organic Matter is lignin and concentration sugar
Sweet bacterium solution.
6. organic master batch according to claim 1, it is characterised in that described nutrient is N, P2O5And K2O。
7. organic master batch according to claim 5, it is characterised in that described lignin by complete water-soluble lignin Jing 50～
Prepare after 58 DEG C of 7～10d that become thoroughly decomposed.
8. organic master batch according to claim 5, it is characterised in that described concentration molasses bacterium solution is prepared by the following method
Obtain：
By bacillus subtilis and/or bacillusmusilaginosiengineering Jing after seed culture medium culture, connect with the ratio of 10～20%v/v
Enter fermented and cultured in fermentation medium so that living bacteria count reaches 100～30,000,000,000/g in zymotic fluid；Cane molasses are prepared
Into after the molasses aqueous solution of mass percent 50～60%, zymotic fluid is added into the molasses aqueous solution with 10～20% volume ratio
In, 30～40 DEG C of bottom fermentations are obtained final product for 2～3 days.
9. the preparation method of the organic master batch described in claim 1, it is characterised in that it comprises the steps：
By granulating of reuniting in ammonium sulfate, MAP, concentration molasses bacterium solution, lignin and polyglutamic acid input comminutor, obtain
Granulating wet stock Jing hardening by cooling, drying and sieve after, obtain final product.
10. preparation method according to claim 9, it is characterised in that in organic master batch, the mass percent of ammonium sulfate is
15～20%, the mass percent of MAP is 3～8%, and the mass percent for concentrating molasses bacterium solution is 15～20%, wooden
The mass percent of element is 50～62%, and the mass percent of polyglutamic acid is 0.05～-0.06%.
11. preparation methods according to claim 9, it is characterised in that in comminutor, reaction temperature is 70～80 DEG C.
Application of the organic master batch described in 12. claims 1 in crop yield is improved.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108794187A (en) * | 2018-07-24 | 2018-11-13 | 南京轩凯生物科技有限公司 | A kind of microbial liquid microbial inoculum and its preparation method and application |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104591887A (en) * | 2014-12-31 | 2015-05-06 | 山东宝源生物有限公司 | Compound microbial fertilizer having high nutrient content and preparation method thereof |
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2016
- 2016-12-19 CN CN201611178333.9A patent/CN106631446B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104591887A (en) * | 2014-12-31 | 2015-05-06 | 山东宝源生物有限公司 | Compound microbial fertilizer having high nutrient content and preparation method thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108794187A (en) * | 2018-07-24 | 2018-11-13 | 南京轩凯生物科技有限公司 | A kind of microbial liquid microbial inoculum and its preparation method and application |
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Address after: 210000 Building 10, Building A, Phase I, Zhongdan Life Science Industrial Park, 3-1 Xinjinhu Road, Jiangbei New District, Nanjing City, Jiangsu Province Applicant after: Nanjing xuankai Biotechnology Co., Ltd Address before: New Kumho 210000 Nanjing Road, Jiangsu province No. 3-1 in life science industrial park A building 10 floor Applicant before: NANJING SHINEKING BIOTECH Co.,Ltd. |
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