CN106619440A - Herba Dendrobii combined eye cream with effect of promoting skin regeneration - Google Patents
Herba Dendrobii combined eye cream with effect of promoting skin regeneration Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/981—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of mammals or bird
- A61K8/982—Reproductive organs; Embryos, Eggs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/671—Vitamin A; Derivatives thereof, e.g. ester of vitamin A acid, ester of retinol, retinol, retinal
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/678—Tocopherol, i.e. vitamin E
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/736—Chitin; Chitosan; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/005—Preparations for sensitive skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Abstract
The invention discloses Herba Dendrobii combined eye cream with an effect of promoting skin regeneration. The Herba Dendrobii combined eye cream is prepared from the following raw materials in parts by weight: 15-25 parts of Herba Dendrobii extract, 10-20 parts of vitamin E, 3-6 parts of vitamin D, 5-10 parts of vitamin A, 3-6 parts of water lily fermentation broth, 10-15 parts of placenta extract, 3-7 parts of salmon ovary peptide, 5-10 parts of parsley seed extract, 2-3 parts of grape seed extract, 0.05-0.1 part of cucumber extraction liquid, 1.5-2.5 parts of water-soluble chitosan, 5-10 parts of Radix Ginseng and 5-10 parts of Radix Astragali. The Herba Dendrobii combined eye cream with the effect of promoting skin regeneration has remarkable functions of promoting cell neogenesis and repairing and reestablishing tissues, and has good moisturizing, ageing resisting and repairing functions.
Description
Technical field
The present invention relates to skin care field, and in particular to a kind of Dendrobidium huoshanness promotes skin regeneration combination eye cream.
Background technology
Sensitive skin has following characteristics mostly；
1st, epiderm skin is thin, and capilary is obvious；
2nd, skin lacks the easily congested redness of gloss, cheek；
3rd, cause skin that unsure state is easily presented because of seasonal variations；Situation, it may appear that itch, play fash；
4th, cold wind, food, water quality, ultraviolet, synthetic fibers, fragrance are easily received；Or the impact of material.The life of healthy skin
The reason cycle is 28 days, the compact texture of marshalling is formed from basalis to cuticula, there is provided skin is sufficiently protected and moisturizing
Function.Skin possesses normal physiological period, and the cuticula horn cell of the skin the superiors can arrange very neat, and lines is extremely thin
Cause, oil-water balance.The skin metabolism period staggering of sensitive skin, epidermal structure arrangement is mixed and disorderly, Skin Cell also prematurity, natural
When NMF and intercellular lipid are also manufactured without complete, cuticula is just peeled off, and keratoderma cell arrangement is irregular, and
And can up roll up crowded, the moisture content inside skin is easily lost in, the protection without sebum film, and excitor substance or allergin are easily invaded
Enter, cause skin inside inflammation.
At present, the product that on the market most of antiallergic senses are repaired, anti-inflammatory of only calming, this has side to immediate allergic skin
Help, but to the conditioning of sensitive in fact use less, and also cannot be truly the reparative regeneration for realizing skin.
Dendrobidium huoshanness, is the superfine product in the stem of noble dendrobium also known as rice dry measure used in former times, is the endangered rare medicinal material of China national.Its drying
Stem (Huo Fengdou) and fresh dry measure used in former times can be used as medicine.
《Compendium of Materia Medica》Record：The stem of noble dendrobium is sweet flat nontoxic.In curing mainly wound, except gas under numbness, tonifying five zang organs consumptive disease wins thin, Qiang Yinyi
Essence, long term usage thickness stomach, never sufficient in benefit, flat stomach gas, long muscle, by skin heat symptoms caused by an exopathgen miliaria gas, it is weak that pin knee aches cold numbness.Sedate removes frightened,
Make light of one's life by commiting suicide macrobiosis, beneficial gas heat extraction, it is weak to control man's waist pin, is good for sun.Cold long in bone by skin wandering arthritis, kidney tonifying benefit power strengthens the bone, and warms up
Water is dirty, the clear gas of intelligence development, controls plug in heating spontaneous perspiration, ulcer apocenosis.
Suddenly dry measure used in former times is rich in more than ten kinds of alkaloids such as polysaccharide, amino acid and dendrobine, stem of noble dendrobium amine bases.Jing Chinese Academy of Medical Sciences medicine
Identified with Institute of Zoology：Polyoses content is 18.974%, and total alkaloid content is 0.030%.Immunity of organism can be effectively improved
Function, to human body eye, pharynx, lung.The disease such as the organs such as stomach, intestines, kidney and blood, angiocarpy has special efficacy.Energy anti-cataract, prolongs
Slow aging, anti-mutation is antitumor.
The content of the invention
To solve the above problems, the invention provides a kind of Dendrobidium huoshanness promotes skin regeneration combination eye cream, can promote thin
The new life of born of the same parents and reconstruction are organized.
For achieving the above object, the technical scheme taken of the present invention is：
A kind of Dendrobidium huoshanness promotes skin regeneration combination eye cream, is prepared from by the raw material of following weight portion：
Dendrobidium huoshanness extract 15-25 parts, vitamin e 10-20 parts, vitamin D 3-6 parts, Alphalin 5-10 parts, water
Lotus flower fermentate 3-6 parts, placental hormone 10-15 parts, salmon ovary peptide 3-7 parts, Sheep's-parsley seed extract 5-10 parts, grape seed extract
2-3 parts, cucumber extract 0.05-0.1 parts, water soluble chitosan 1.5-2.5 parts, ginseng 5-10 parts, Radix Astragali 5-10 parts.
Preferably, the water lotus flower yeast powder is as obtained by prepared by following steps：
Take the water lotus flower cleaned after draining to be placed in steam-explosion jar, it is 0.7- to be first passed through nitrogen to steam explosion pressure inside the tank
1.5MPa, explosion treatment 7-23min；Then it is 1.5-2.1MPa to be passed through steam rapidly to steam explosion pressure inside the tank, at steam blasting
After reason 0.8-2.8min, according to the ratio microbe inoculation leavening of inoculum concentration 1.5-1.7%, keeping temperature 25-35 DEG C, fermentation
After 48-54 hours, high-temperature sterilization, freeze-drying obtains water lotus flower yeast powder.
Preferably, the microbe leaven is by Bacillus acidi lactici, bacillus licheniformis, Corynebacterium glutamicum in mass ratio 3:
2:1 mixing gained.
Preferably, the Dendrobidium huoshanness extract is as obtained by prepared by following steps：
S1, the Dendrobidium huoshanness that drains will be cleaned it is placed in steam-explosion jar, it is 0.6- to be first passed through nitrogen to steam explosion pressure inside the tank
1.4MPa, explosion treatment 8-25min；Then it is 1.4-1.8MPa to be passed through steam rapidly to steam explosion pressure inside the tank, at steam blasting
After reason 0.5-2.5min, room temperature is concentrated into relative density 1.10-1.20, is spray-dried concentrate, obtains powder；
S2, in powder add 3-8 times of water, by mass percentage be 0.5-2% ratio add neutral proteinase, 30-
0.5-2h is digested at 50 DEG C, is filtered, obtain enzymolysis liquid；
S3, by naturally to thaw again after the zymotic fluid quick freeze of gained, make age of starch precipitation in extract；Collect supernatant
Liquid, remainder centrifugation is collected centrifugal liquid and is incorporated in supernatant；
S4, by supernatant with the flow velocity of 3-7BV/h by macroporous resin column, after Dynamic Adsorption saturation, given birth to epidermal cell
The long factor is colourless to efflux with the above-mentioned macroporous resin column of flow velocity drip washing of 5-8BV/h, then is 50%-70%'s with volume fraction
Organic solvent is eluted with the flow velocity of 8-12BV/h, collects eluent, and reduced pressure concentration obtains medicinal extract；
S5, will after medicinal extract water dissolves with the flow velocity of 3-5BV/h by polyamide column, after Dynamic Adsorption saturation, use 3-7BV
Epithelical cell growth factor is with the above-mentioned polyamide column of flow velocity drip washing of 2-10BV/h, then is having for 30%-50% with volume fraction
Machine solvent aqueous solution is eluted with the flow velocity of 5-10BV/h, and collection has eluent, is spray-dried, and obtains Dendrobidium huoshanness extract.
Preferably, the cucumber extract is as obtained by prepared by following steps：
90% methyl alcohol or ethanol solution of 6 times of volumes will be added after fresh cucumber ultramicro grinding, process 1-3 minutes, Gu
After liquid is separated, methyl alcohol or ethanol solution, concentration are reclaimed so that extract is in paste, then with water-saturated n-butanol to extract
Extraction 3 times, after recycling design, obtains cucumber extract.
Preferably, the Sheep's-parsley seed extract is as obtained by prepared by following steps：
Caraway seed is cleaned and is placed in steam-explosion jar after draining, it is 0.6-1.4MPa to be first passed through nitrogen to steam explosion pressure inside the tank,
Explosion treatment 8-25min；Then it is 1.4-1.8MPa, Steam explosion treatment 0.5- to be passed through steam to steam explosion pressure inside the tank rapidly
After 2.5min, room temperature is down to, adds 90% ethanol solution of 6-10 times of volume, process 1-3 minutes, after separation of solid and liquid, reclaim second
Alcoholic solution, concentration so that extract is in paste, then is extracted 3 times to extract with water-saturated n-butanol, after recycling design, will
Extract drying concentrated in vacuo, obtains Sheep's-parsley seed extract.
The invention has the advantages that：
By water lotus flower yeast powder, placental hormone and salmon ovary peptide and homemade Dendrobidium huoshanness extract, cucumber extract
With the synergy of Sheep's-parsley seed extract so that the eye cream of gained has the new life and reconstruction tissue of significant promotion cell
Function；The reasonable addition of collaboration vitamin D, grape seed extract etc. so as to preferable moisturizing, anti-ageing and reparation work(
Energy.
Specific embodiment
In order that objects and advantages of the present invention become more apparent, the present invention is carried out further with reference to embodiments
Describe in detail.It should be appreciated that specific embodiment described herein is not used to limit this only to explain the present invention
It is bright.
In following examples, the water lotus flower yeast powder for being used is as obtained by prepared by following steps：
Take the water lotus flower cleaned after draining to be placed in steam-explosion jar, it is 0.7- to be first passed through nitrogen to steam explosion pressure inside the tank
1.5MPa, explosion treatment 7-23min；Then it is 1.5-2.1MPa to be passed through steam rapidly to steam explosion pressure inside the tank, at steam blasting
After reason 0.8-2.8min, according to the ratio microbe inoculation leavening of inoculum concentration 1.5-1.7%, keeping temperature 25-35 DEG C, fermentation
After 48-54 hours, high-temperature sterilization, freeze-drying obtains water lotus flower yeast powder, wherein, the microbe leaven by Bacillus acidi lactici,
Bacillus licheniformis, Corynebacterium glutamicum in mass ratio 3:2:1 mixing gained.
The Dendrobidium huoshanness extract for being used is as obtained by prepared by following steps：
S1, the Dendrobidium huoshanness that drains will be cleaned it is placed in steam-explosion jar, it is 0.6- to be first passed through nitrogen to steam explosion pressure inside the tank
1.4MPa, explosion treatment 8-25min；Then it is 1.4-1.8MPa to be passed through steam rapidly to steam explosion pressure inside the tank, at steam blasting
After reason 0.5-2.5min, room temperature is concentrated into relative density 1.10-1.20, is spray-dried concentrate, obtains powder；
S2, in powder add 3-8 times of water, by mass percentage be 0.5-2% ratio add neutral proteinase, 30-
0.5-2h is digested at 50 DEG C, is filtered, obtain enzymolysis liquid；
S3, by naturally to thaw again after the zymotic fluid quick freeze of gained, make age of starch precipitation in extract；Collect supernatant
Liquid, remainder centrifugation is collected centrifugal liquid and is incorporated in supernatant；
S4, by supernatant with the flow velocity of 3-7BV/h by macroporous resin column, after Dynamic Adsorption saturation, given birth to epidermal cell
The long factor is colourless to efflux with the above-mentioned macroporous resin column of flow velocity drip washing of 5-8BV/h, then is 50%-70%'s with volume fraction
Organic solvent is eluted with the flow velocity of 8-12BV/h, collects eluent, and reduced pressure concentration obtains medicinal extract；
S5, will after medicinal extract water dissolves with the flow velocity of 3-5BV/h by polyamide column, after Dynamic Adsorption saturation, use 3-7BV
Epithelical cell growth factor is with the above-mentioned polyamide column of flow velocity drip washing of 2-10BV/h, then is having for 30%-50% with volume fraction
Machine solvent aqueous solution is eluted with the flow velocity of 5-10BV/h, and collection has eluent, is spray-dried, and obtains Dendrobidium huoshanness extract.
The cucumber extract for being used is as obtained by prepared by following steps：
90% methyl alcohol or ethanol solution of 6 times of volumes will be added after fresh cucumber ultramicro grinding, process 1-3 minutes, Gu
After liquid is separated, methyl alcohol or ethanol solution, concentration are reclaimed so that extract is in paste, then with water-saturated n-butanol to extract
Extraction 3 times, after recycling design, obtains cucumber extract.
The Sheep's-parsley seed extract for being used is as obtained by prepared by following steps：
Caraway seed is cleaned and is placed in steam-explosion jar after draining, it is 0.6-1.4MPa to be first passed through nitrogen to steam explosion pressure inside the tank,
Explosion treatment 8-25min；Then it is 1.4-1.8MPa, Steam explosion treatment 0.5- to be passed through steam to steam explosion pressure inside the tank rapidly
After 2.5min, room temperature is down to, adds 90% ethanol solution of 6-10 times of volume, process 1-3 minutes, after separation of solid and liquid, reclaim second
Alcoholic solution, concentration so that extract is in paste, then is extracted 3 times to extract with water-saturated n-butanol, after recycling design, will
Extract drying concentrated in vacuo, obtains Sheep's-parsley seed extract.
The organic solvent for being used is the turpentine derivatives of natural origin, and the turpentine derivatives are different for firpene
Structure, disproportionation products.
Other materials is commercially available.
Embodiment 1
A kind of Dendrobidium huoshanness promotes skin regeneration combination eye cream, is prepared from by the raw material of following weight portion：
Dendrobidium huoshanness extract 15-25 parts, vitamin e 10-20 parts, vitamin D 3-6 parts, Alphalin 5-10 parts, water
Lotus flower fermentate 3-6 parts, placental hormone 10-15 parts, salmon ovary peptide 3-7 parts, Sheep's-parsley seed extract 5-10 parts, grape seed extract
2-3 parts, cucumber extract 0.05-0.1 parts, water soluble chitosan 1.5-2.5 parts, ginseng 5-10 parts, Radix Astragali 5-10 parts.
Embodiment 2
A kind of Dendrobidium huoshanness promotes skin regeneration combination eye cream, is prepared from by the raw material of following weight portion：
Dendrobidium huoshanness extract 15-25 parts, vitamin e 10-20 parts, vitamin D 3-6 parts, Alphalin 5-10 parts, water
Lotus flower fermentate 3-6 parts, placental hormone 10-15 parts, salmon ovary peptide 3-7 parts, Sheep's-parsley seed extract 5-10 parts, grape seed extract
2-3 parts, cucumber extract 0.05-0.1 parts, water soluble chitosan 1.5-2.5 parts, ginseng 5-10 parts, Radix Astragali 5-10 parts.
Embodiment 3
A kind of Dendrobidium huoshanness promotes skin regeneration combination eye cream, is prepared from by the raw material of following weight portion：
Dendrobidium huoshanness extract 15-25 parts, vitamin e 10-20 parts, vitamin D 3-6 parts, Alphalin 5-10 parts, water
Lotus flower fermentate 3-6 parts, placental hormone 10-15 parts, salmon ovary peptide 3-7 parts, Sheep's-parsley seed extract 5-10 parts, grape seed extract
2-3 parts, cucumber extract 0.05-0.1 parts, water soluble chitosan 1.5-2.5 parts, ginseng 5-10 parts, Radix Astragali 5-10 parts.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (6)
1. a kind of Dendrobidium huoshanness promotes skin regeneration combination eye cream, it is characterised in that be prepared from by the raw material of following weight portion：
Dendrobidium huoshanness extract 15-25 parts, vitamin e 10-20 parts, vitamin D 3-6 parts, Alphalin 5-10 parts, water lotus flower
Fermentate 3-6 parts, placental hormone 10-15 parts, salmon ovary peptide 3-7 parts, Sheep's-parsley seed extract 5-10 parts, grape seed extract 2-3
Part, cucumber extract 0.05-0.1 parts, water soluble chitosan 1.5-2.5 parts, ginseng 5-10 parts, Radix Astragali 5-10 parts.
2. a kind of Dendrobidium huoshanness as claimed in claim 1 promotes skin regeneration combination eye cream, it is characterised in that the water lotus flower
Yeast powder is as obtained by prepared by following steps：
Take the water lotus flower cleaned after draining to be placed in steam-explosion jar, it is 0.7-1.5MPa to be first passed through nitrogen to steam explosion pressure inside the tank, quick-fried
Tear manages 7-23min；Then it is 1.5-2.1MPa, Steam explosion treatment 0.8- to be passed through steam to steam explosion pressure inside the tank rapidly
After 2.8min, according to the ratio microbe inoculation leavening of inoculum concentration 1.5-1.7%, keeping temperature 25-35 DEG C, ferment 48-54
After hour, high-temperature sterilization, freeze-drying obtains water lotus flower yeast powder.
3. a kind of Dendrobidium huoshanness as claimed in claim 1 promotes skin regeneration combination eye cream, it is characterised in that the microorganism
Leavening is by Bacillus acidi lactici, bacillus licheniformis, Corynebacterium glutamicum in mass ratio 3:2:1 mixing gained.
4. a kind of Dendrobidium huoshanness as claimed in claim 1 promotes skin regeneration combination eye cream, it is characterised in that the Huoshan stone
Dry measure used in former times extract is as obtained by prepared by following steps：
S1, the Dendrobidium huoshanness that drains will be cleaned it is placed in steam-explosion jar, it is 0.6-1.4MPa to be first passed through nitrogen to steam explosion pressure inside the tank,
Explosion treatment 8-25min；Then it is 1.4-1.8MPa, Steam explosion treatment 0.5- to be passed through steam to steam explosion pressure inside the tank rapidly
After 2.5min, room temperature is concentrated into relative density 1.10-1.20, is spray-dried concentrate, obtains powder；
S2, in powder add 3-8 times of water, by mass percentage be 0.5-2% ratio add neutral proteinase, 30-50 DEG C
Lower enzymolysis 0.5-2h, filters, and obtains enzymolysis liquid；
S3, by naturally to thaw again after the zymotic fluid quick freeze of gained, make age of starch precipitation in extract；Collect supernatant,
Remainder is centrifuged, and collects centrifugal liquid and is incorporated in supernatant；
S4, by supernatant with the flow velocity of 3-7BV/h by macroporous resin column, after Dynamic Adsorption saturation, use epidermal growth because
Son is colourless to efflux with the above-mentioned macroporous resin column of flow velocity drip washing of 5-8BV/h, then is the organic of 50%-70% with volume fraction
Solvent is eluted with the flow velocity of 8-12BV/h, collects eluent, and reduced pressure concentration obtains medicinal extract；
S5, will after medicinal extract water dissolves with the flow velocity of 3-5BV/h by polyamide column, after Dynamic Adsorption saturation, use 3-7BV epidermises
Porcine HGF is with the above-mentioned polyamide column of flow velocity drip washing of 2-10BV/h, then is the organic molten of 30%-50% with volume fraction
The agent aqueous solution is eluted with the flow velocity of 5-10BV/h, and collection has eluent, is spray-dried, and obtains Dendrobidium huoshanness extract.
5. a kind of Dendrobidium huoshanness as claimed in claim 1 promotes skin regeneration combination eye cream, it is characterised in that the cucumber extraction
Take liquid as following steps prepare obtained by：
90% methyl alcohol or ethanol solution of 6 times of volumes will be added after fresh cucumber ultramicro grinding, process 1-3 minutes, solid-liquid point
From rear, methyl alcohol or ethanol solution, concentration are reclaimed so that extract is in paste, then with water-saturated n-butanol to extract extraction 3
It is secondary, after recycling design, obtain cucumber extract.
6. a kind of Dendrobidium huoshanness as claimed in claim 1 promotes skin regeneration combination eye cream, it is characterised in that the caraway seed
Extract is as obtained by prepared by following steps：
By caraway seed clean drain after be placed in steam-explosion jar, be first passed through nitrogen to steam explosion pressure inside the tank be 0.6-1.4MPa, explosion
Process 8-25min；Then it is 1.4-1.8MPa, Steam explosion treatment 0.5-2.5min to be passed through steam to steam explosion pressure inside the tank rapidly
Afterwards, room temperature is down to, 90% ethanol solution of 6-10 times of volume is added, 1-3 minutes are processed, after separation of solid and liquid, ethanol solution is reclaimed,
Concentration so that extract is in paste, then is extracted 3 times to extract with water-saturated n-butanol, after recycling design, extract is true
Empty concentrate drying, obtains Sheep's-parsley seed extract.
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Application publication date: 20170510 |