CN106617043A - Preparation method and application of bloom cyanobacteria nutrient solution - Google Patents
Preparation method and application of bloom cyanobacteria nutrient solution Download PDFInfo
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- CN106617043A CN106617043A CN201610840310.3A CN201610840310A CN106617043A CN 106617043 A CN106617043 A CN 106617043A CN 201610840310 A CN201610840310 A CN 201610840310A CN 106617043 A CN106617043 A CN 106617043A
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention discloses a preparation method of a bloom cyanobacteria nutrient solution. The preparation comprises the steps of putting bloom cyanobacteria into a containing space with certain volume, introducing 5g/m<3> of ozone to the bottom of the containing space, and sterilizing for 8-10h, meanwhile, illuminating for 36-48h by adopting 6000-200001x light beams, then adding a leavening agent, fermenting for 62-74h, and finally carrying out aftertreatment to prepare the bloom cyanobacteria nutrient solution. The invention also provides application of the bloom cyanobacteria nutrient solution prepared by the preparation method in the aspects of feed preparation and enhancement of immunity. According to the invention, the bloom cyanobacteria which damages water quality and endangers water body is utilized by applying a biological engineering technology, the nutrient substances in the bloom cyanobacteria are extracted, and used as the raw materials of the poultry and livestock feed, so that the waste material is changed into things of value, not only can the damage of the bloom cyanobacteria to the environment be alleviated, but also the feeding cost is lowered, and the cultivation income is increased. The bloom cyanobacteria nutrient solution also can be used as a medicament for improving the immunity of animals.
Description
Technical field
Present invention relates particularly to technical field of bioengineering, the preparation method of more particularly to a kind of bloom blue algae nutrient solution and
Its application.
Background technology
Bloom blue algae, a kind of non-blue-green algae, but by microcystis kutz（Microcystis）, Anabaena（Anobaena）, read
Pearl Trentepohlia（Nostoc）, Oscillatoria（Osclltoria）, blue-green algae（Cyanophyte）And green alga（Chlorophyceae）Deng many
Kind algae is mixed to get together, and floats on the algae colony of the water surface.
Above-described algae colony is into polluted water region, destruction water quality, water ecological environment and impact industrial and agricultural production
Key factor, thus resulted in huge economic loss, become the killer being detrimental to health, and its harmfulness day is increasingly
It is acute.Since more than ten years, people take substantial amounts of financial resources, material resources, manpower and they are prevented and treated, regrettably to these algae
Result of preventing and treating be：Control more and more, water pollution is increasingly severe, so preventing and treating " bloom blue algae " has become quite thorny difficulty
Topic.
Form " bloom blue algae " is got together by various blue-green algaes in algae and other algaes are mixed, wherein in each category just
There are various algae, they are both can to destroy water quality with polluted-water, and containing various useful (composition) materials, are such as applied,
Can turn waste into wealth.Such as Oscillatoria, just there are various 86 kinds of an algaes that quiver, Ru Ana quivers algae（O.annae）, mud life quivers algae
（O.limosa）, creep the algae that quivers（O.animalis）Deng；There is 12 kinds of micro-capsule kind in Microcystis aeruginosa generic, such as microcystic aeruginosa
（M.aeruginosa）, thick Microcystis aeruginosa（M.robasta）And Anabaena Flos-aquae（A.flas-auae）, Nostoc Commune
（N.commune）, hair-like nostoc（N.fllagelliforme）Etc., remaining algae repeats no more）Though these algae pollutions
Water body, destroys water quality, but also all respectively has characteristic.Below citing is illustrated：
1）Quiver algae：40wt% containing protein or so, 18 kinds of amino acid, multivitamin, mineral element and bioactivator.
1986, bis- professors of Harvard Medical School of the U.S. Schwarta and Shklar had found that one kind does not know kind of a name in Oscillariaceae
Algae extract --- algae albumen has inhibitory action to some cancer cells, and used anti-aging and treatment AIDS sick.Opened by this
Send out, we set about test in 1989, as a result show that mud is given birth to the algae that quivers and quivers algae (O.pianctonica) with AntiHIV1 RT activity work with swimming
Property.
2）Microcystis aeruginosa：47wt% containing protein or so, 18 kinds of amino acid, multivitamin, mineral element.We are more than 30
Year applies algae purification（Process sewage）During, Microcystis aeruginosa is had been found that Jing after hydrolysis " detoxification ", containing asparatate, silk
Propylhomoserin, a word used in person's names propylhomoserin, alanine, leucine, arginine, these amino acid some of which are the necessary 8 kinds of amino acid of body, can be used
In the raw material of chemical industry fertilizer.
3）Nostoc：China has 31 kinds, is grown in various water bodys and moist place with the presence of it, is referred to as wherein having and planting
The nostoc of " nostoc ", with food therapy effect, can improve body immunity.One kind is referred to as Nostoc Commune
（N.commune）It is commonly called as " Nostoc commune " and hair-like nostoc（N.fllagelliforme）" delivering vegetables " is commonly called as, all because it contains battalion
Support abundant, liked by temperature-sensitive by deep as delicious food.
4）Anabena：There are 32 kinds and 15 mutation in China, is also that " bloom blue algae " plants common species, still there is various green
Algae, such as chlorella（Chloreella）, Gate algaes（Scenedsmbxs）Deng all containing abundant nutriment, these species algae
Early in the fifties in last century by China algology researcher, artificial large area culture is used successfully to food, is that part is alleviated
Disaster famine in 3 years plays a role, and is put into 1962《Hubei pharmacopeia》It is interior.And diatom, the various algaes of hidden algae etc.
Class mixes wherein, forms algae group, is corruptly called " bloom blue algae ".
In sum, since bloom blue algae has many abundant nutriments, if being used, not only can reduce
Harm of the algae to environment, and can turn waste into wealth, thus the preparation method and applications of bloom blue algae nutrient solution how are realized,
Become the emphasis of those skilled in the art's study.
The content of the invention
Present invention is primarily targeted at providing a kind of preparation method and applications of bloom blue algae nutrient solution, algae is reduced
Harm to the pollution of environment, and these resources are given into utilization, turns harm into good, and turns waste into wealth, with overcome it is of the prior art not
Foot.
To realize aforementioned invention purpose, the technical solution used in the present invention includes：
A kind of preparation method of bloom blue algae nutrient solution, including：
Have in the receiving space of a constant volume one and put into bloom blue algae, be passed through 5g/m3's in the bottom of the receiving space
Ozone carries out sterilization processing 8-10h, meanwhile, 36-48h is shone using the light beam light of 6000-200001x, then, add leavening pair
Bloom blue algae carries out fermentation process 62-74h, finally, carries out post processing and the bloom blue algae nutrient solution is obtained.
Preferably, the mass fraction that the quality of the leavening accounts for the bloom blue algae is 35wt%-45wt%.
In some more preferred embodiment, the preparation method of the leavening includes：
To include at least：Lime 41-43wt%, urea 20-24wt%, sugar 2-3wt%, salt 2-4wt%, monosodium glutamate 6-8wt% are little
Soda 22-24wt%, micro- 0.5wt%, carbohydrase 30-32wt%, proteinase 8-10wt%, saccharomycete 15-17wt%, fiber
Plain enzyme 25-27wt%, the mixture of lactic acid bacteria 10-12wt% is stirred, and then, carries out pulverization process, then crosses 100 mesh
After sieve, the leavening of fine-powdered is made.
Further, the trace element at least includes Cu, Na, K, Mn, Fe, Zn, one or more groups in Co
Close.
In some more preferred embodiment, the receiving space is the heatable adobe sleeping platform that nearly waterfront is excavated out, the heatable adobe sleeping platform
Bottom and four walls laying plastic film.
Further, the heatable adobe sleeping platform is the heatable adobe sleeping platform of each 0.5m of length.
Further, the length for putting into the bloom blue algae in the heatable adobe sleeping platform is respectively 0.4m.
The bloom blue algae nutrient solution that the method for being prepared as described above is prepared is in the one of which as livestock rearing feed point
On application.
Application of the bloom blue algae nutrient solution that the method for being prepared as described above is prepared on immunity of organisms is strengthened.
Compared with prior art, applied bioengineering technology of the present invention will destroy water quality, the bloom blue algae of harm water body adds
To utilize, nutriment therein is extracted, it is possible to as the raw material of poultry and livestock feed, such that it is able to turn waste into wealth,
Harm of the bloom blue algae to environment is not only reduced, feeding cost can also be reduced, increase cultivation income；The nutrition of the bloom blue algae
Liquid is also used as the medicament of animal strengthen immunity.
Specific embodiment
In view of deficiency of the prior art, inventor Jing studies for a long period of time and puts into practice in a large number, is able to propose the present invention's
Technical scheme.The technical scheme, its implementation process and principle etc. will be further explained as follows.
A kind of preparation method of bloom blue algae nutrient solution provided in an embodiment of the present invention, including：
Have in the receiving space of a constant volume one and put into bloom blue algae, be passed through 5g/m3's in the bottom of the receiving space
Ozone carries out sterilization processing 8-10h, meanwhile, 36-48h is shone using the light beam light of 6000-200001x, then, add leavening pair
Bloom blue algae carries out fermentation process 62-74h, finally, carries out post processing and the bloom blue algae nutrient solution is obtained.
Preferably, the mass fraction that the quality of the leavening accounts for the bloom blue algae is 35wt%-45wt%.
In some more preferred embodiment, the preparation method of the leavening includes：
To include at least：Lime 41-43wt%, urea 20-24wt%, sugar 2-3wt%, salt 2-4wt%, monosodium glutamate 6-8wt% are little
Soda 22-24wt%, micro- 0.5wt%, carbohydrase 30-32wt%, proteinase 8-10wt%, saccharomycete 15-17wt%, fiber
Plain enzyme 25-27wt%, the mixture of lactic acid bacteria 10-12wt% is stirred, and then, carries out pulverization process, then crosses 100 mesh
After sieve, the leavening of fine-powdered is made.
Further, the trace element at least includes Cu, Na, K, Mn, Fe, Zn, one or more groups in Co
Close.
In some more preferred embodiment, the receiving space is the heatable adobe sleeping platform that nearly waterfront is excavated out, the heatable adobe sleeping platform
Bottom and four walls laying plastic film.
Further, the heatable adobe sleeping platform is the heatable adobe sleeping platform of each 0.5m of length.
Further, the length for putting into the bloom blue algae in the heatable adobe sleeping platform is respectively 0.4m.
The bloom blue algae nutrient solution that the method for being prepared as described above is prepared is in the one of which as livestock rearing feed point
On application.
Application of the bloom blue algae nutrient solution that the method for being prepared as described above is prepared on immunity of organisms is strengthened.
Applied bioengineering technology of the present invention will destroy water quality, the bloom blue algae of harm water body is used, will be therein
Nutriment is extracted, it is possible to used as the raw material of poultry and livestock feed, such that it is able to turn waste into wealth, not only reduces bloom blue algae
Harm to environment, can also reduce feeding cost, increase cultivation income；The nutrient solution of the bloom blue algae is also used as animal
The medicament of strengthen immunity.
Below in conjunction with the technical solution of the present invention is further explained the explanation of some embodiments.
Embodiment 1：
The preparation method of bloom blue algae nutrient solution：
The heatable adobe sleeping platform of each 0.5m of length is dug on offshore side, in its bottom and four walls laying plastic film, by the wawter bloom fished in water indigo plant
In algae input hole, the length of bloom blue algae is respectively 0.4m or so, and in algae heap bottom the ozone of 5g/m3 is passed through, and is carried out continuously
Sterilization processing 8-10h, meanwhile, 36-48h is shone using the light beam light of 6000-200001x, then, leavening is added to bloom blue algae
Fermentation process 62-74h is carried out, finally, post processing is carried out and the bloom blue algae nutrient solution is obtained.
Wherein, the preparation method of leavening includes：To include at least：Lime 42wt%, urea 22wt%, sugar 2.5wt%,
Salt 3.5wt%, monosodium glutamate 7wt%, sodium bicarbonate 23wt%, micro- 0.5wt%, carbohydrase 31wt%, proteinase 9 wt%, saccharomycete
16wt%, cellulase 26wt%, the mixture of lactic acid bacteria 11wt% is stirred, and then, carries out pulverization process, then mistake
After 100 mesh sieves, the leavening of fine-powdered is made.
More specifically, trace element is comprising at least including Cu, Na, K, Mn, Fe, Zn, one or more in Co
Combination.
The nutrient component meter of bloom blue algae nutrient solution is as follows：
The nutriment of table 1（wt%）
| Protein（Amino acid） | 40wt%-50wt% |
| Carbohydrate | 12%wt-14wt% |
| Fat | 12wt%-23wt% |
| Mineral matter | 2wt%-4wt% |
| Ash content | 2wt%-3wt% |
The mineral matter of table 2 and trace element (mg/Kg）
| Ca | 890-1100 |
| Mg | 65-72 |
| Fe | 368-445 |
| Zn | 16-18 |
| K | 765-934 |
| Mn | 8-12 |
| Na | 134-225 |
| Other（Germanium, selenium etc.） | 28-36 |
Vitamin (the Cg/Kg of table 3）
| Beta carotene | 0.8-1.4 |
| VB1 | 23-30 |
| VB2 | 14-18 |
| VB6 | 1.5-2.0 |
| VB12 | 0.4-0.6 |
| Algocyan（It is blue） | 0.8-1.1/10g |
| Chlorophyll a (green) | 0.1-0.12/10g |
Embodiment 2：
Application of the bloom blue algae nutrient solution on feed obtained in embodiment 1 be：Using above-described bloom blue algae nutrient solution
The feed to form domestic birds and animals etc. is prepared as raw material, i.e. by bloom blue algae nutrient solution and other raw materials according to certain proportioning
The step such as mixed, solidified, so as to form feed.
Embodiment 3：
Application of the bloom blue algae nutrient solution in terms of immunity of organism obtained in embodiment 1 be：Can be used as the body for improving animal
The injection of immunity, will bloom blue algae nutrient solution and other raw materials mixed and allocated according to certain proportioning, so as to
Form injection.
Below again by the nontoxicitys of two test explanation bloom blue algae nutrient solutions, so as to illustrate it in above-mentioned application
On feasibility, it is specific as follows：
Bloom blue algae nutrient solution obtained in the present embodiment 1 carries out acute toxicity test：
Prepare before test：Kunming mice is provided（Suzhou Medical College animal section）：20.9 ± 1.88 grams of body weight is female, male mouse each 10
Only；Bloom blue algae nutrient solution obtained in the present embodiment, plus distilled water are prepared into 15wt% suspensions.
Process of the test：15wt% suspensions obtained above are poured into 20 mouse bodies, the consumption of every mouse is
0.04mL/g, in normal rearing conditions Continuous Observation seven days, has no that mouse has exception or the phenomena of mortality.
The immunity test of bloom blue algae nutrient solution obtained in embodiment 1：
Male mice is taken, body weight 20-22g causes injury method for 5%II burns, the wawter bloom per only daily hypodermic injection 4mg/ only once
Blue-green alga extract, totally 6 times, as a result such as following table：
The immunity of the bloom blue algae extract of table 4 tests totally 6 results
As shown in Table 4：Bloom blue algae extract has abundant nutriment, without acute toxicity, the work with strengthen immunity
With can apply on immunity of organism.Additionally, bloom blue algae extract can be used as the raw material of poultry and livestock feed.
It should be appreciated that above-described embodiment technology design only to illustrate the invention and feature, its object is to allow and are familiar with this
The personage of item technology will appreciate that present disclosure and implement according to this, can not be limited the scope of the invention with this.It is all
The equivalence changes made according to spirit of the invention or modification, all should be included within the scope of the present invention.
Claims (9)
1. a kind of preparation method of bloom blue algae nutrient solution, it is characterised in that include：
Have in the receiving space of a constant volume one and put into bloom blue algae, be passed through 5g/m3's in the bottom of the receiving space
Ozone carries out sterilization processing 8-10h, meanwhile, 36-48h is shone using the light beam light of 6000-200001x, then, add leavening pair
Bloom blue algae carries out fermentation process 62-74h, finally, carries out post processing and the bloom blue algae nutrient solution is obtained.
2. the preparation method of bloom blue algae nutrient solution according to claim 1, it is characterised in that：The quality of the leavening
The mass fraction for accounting for the bloom blue algae is 35wt%-45wt%.
3. the preparation method of bloom blue algae nutrient solution according to claim 1, it is characterised in that：The preparation of the leavening
Method includes：
To include at least：Lime 41-43wt%, urea 20-24wt%, sugar 2-3wt%, salt 2-4wt%, monosodium glutamate 6-8wt% are little
Soda 22-24wt%, micro- 0.5wt%, carbohydrase 30-32wt%, proteinase 8-10wt%, saccharomycete 15-17wt%, fiber
Plain enzyme 25-27wt%, the mixture of lactic acid bacteria 10-12wt% is stirred, and then, carries out pulverization process, then crosses 100 mesh
After sieve, the leavening of fine-powdered is made.
4. the preparation method of bloom blue algae nutrient solution according to claim 3, it is characterised in that：The trace element is at least
Including Cu, Na, K, Mn, Fe, Zn, one or more combinations in Co.
5. the preparation method of bloom blue algae nutrient solution according to claim 1, it is characterised in that：The receiving space is near
The heatable adobe sleeping platform that waterfront is excavated out, the bottom of the heatable adobe sleeping platform and four walls laying plastic film.
6. the preparation method of bloom blue algae nutrient solution according to claim 5, it is characterised in that：The heatable adobe sleeping platform be length and width,
The heatable adobe sleeping platform of high each 0.5m.
7. the preparation method of bloom blue algae nutrient solution according to claim 6, it is characterised in that：Put into the heatable adobe sleeping platform
The length of bloom blue algae is respectively 0.4m.
8. the bloom blue algae battalion that the preparation method of the bloom blue algae nutrient solution as any one of claim 1-7 is prepared
Application of the nutrient solution in the one of which as livestock rearing feed point.
9. the bloom blue algae battalion that the preparation method of the bloom blue algae nutrient solution as any one of claim 1-7 is prepared
Application of the nutrient solution on immunity of organisms is strengthened.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112299811A (en) * | 2020-01-09 | 2021-02-02 | 兰方环保工程(南京)有限公司 | Soil curing agent for road base and preparation method thereof |
Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1177450A (en) * | 1996-09-26 | 1998-04-01 | 黄志农 | Leaven for straw biological feeds |
| CN101215539A (en) * | 2008-01-07 | 2008-07-09 | 周文彩 | Microorganism bacterium composition, blue algae biological bacterium leaven containing the same, nutriment and biological organic fertilizer, and preparing method thereof |
| CN101337758A (en) * | 2008-08-15 | 2009-01-07 | 江苏省农业科学院 | Method for anaerobic fermentation of bloom blue algae |
| CN101386817A (en) * | 2008-10-31 | 2009-03-18 | 江南大学 | Method for producing yeast single cell protein by blue algae fermentation |
| CN102488084A (en) * | 2011-12-10 | 2012-06-13 | 甘肃鑫盛源牧业科技有限公司 | Method for preparing protein feed by fermentation on straw and cake using aerobic mixed bacteria |
| CN102515945A (en) * | 2011-12-06 | 2012-06-27 | 中国农业科学院农业资源与农业区划研究所 | Algae-enhanced urea, production method thereof, and purpose thereof |
| CN102719383A (en) * | 2012-06-29 | 2012-10-10 | 武汉工业学院 | Bacillus amyloliquefaciens, inoculant and application thereof |
| CN102887735A (en) * | 2012-10-26 | 2013-01-23 | 江汉大学 | Method and fermentation reactor for making organic fertilizer from blue algae |
| CN103232116A (en) * | 2013-04-08 | 2013-08-07 | 南京师范大学 | Method for treating heavy-metal wastewater by using biological water purifying agent prepared from cyanobacteria bloom |
| CN103274852A (en) * | 2013-06-17 | 2013-09-04 | 中国农业科学院农业资源与农业区划研究所 | Algae synergistic ammonium phosphate fertilizer and preparation method thereof |
| CN103749957A (en) * | 2013-12-31 | 2014-04-30 | 宜兴市天石饲料有限公司 | Preparation method for blue-green algae single-cell protein feed |
| CN103773721A (en) * | 2013-12-31 | 2014-05-07 | 陕西万源生物农业科技有限公司 | Composite probiotics for raising pigs |
| CN103783307A (en) * | 2012-10-31 | 2014-05-14 | 张兴文 | Method for producing organic sacchariferous straw feed |
| CN104962490A (en) * | 2015-06-09 | 2015-10-07 | 日照益康有机农业科技发展有限公司 | Enteromorpha microbial agent and preparation method thereof |
| CN105272420A (en) * | 2015-11-13 | 2016-01-27 | 云南云叶化肥股份有限公司 | Organic fertilizer containing blue algae and preparing method of organic fertilizer |
-
2016
- 2016-09-22 CN CN201610840310.3A patent/CN106617043A/en active Pending
Patent Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1177450A (en) * | 1996-09-26 | 1998-04-01 | 黄志农 | Leaven for straw biological feeds |
| CN101215539A (en) * | 2008-01-07 | 2008-07-09 | 周文彩 | Microorganism bacterium composition, blue algae biological bacterium leaven containing the same, nutriment and biological organic fertilizer, and preparing method thereof |
| CN101337758A (en) * | 2008-08-15 | 2009-01-07 | 江苏省农业科学院 | Method for anaerobic fermentation of bloom blue algae |
| CN101386817A (en) * | 2008-10-31 | 2009-03-18 | 江南大学 | Method for producing yeast single cell protein by blue algae fermentation |
| CN102515945A (en) * | 2011-12-06 | 2012-06-27 | 中国农业科学院农业资源与农业区划研究所 | Algae-enhanced urea, production method thereof, and purpose thereof |
| CN102488084A (en) * | 2011-12-10 | 2012-06-13 | 甘肃鑫盛源牧业科技有限公司 | Method for preparing protein feed by fermentation on straw and cake using aerobic mixed bacteria |
| CN102719383A (en) * | 2012-06-29 | 2012-10-10 | 武汉工业学院 | Bacillus amyloliquefaciens, inoculant and application thereof |
| CN102887735A (en) * | 2012-10-26 | 2013-01-23 | 江汉大学 | Method and fermentation reactor for making organic fertilizer from blue algae |
| CN103783307A (en) * | 2012-10-31 | 2014-05-14 | 张兴文 | Method for producing organic sacchariferous straw feed |
| CN103232116A (en) * | 2013-04-08 | 2013-08-07 | 南京师范大学 | Method for treating heavy-metal wastewater by using biological water purifying agent prepared from cyanobacteria bloom |
| CN103274852A (en) * | 2013-06-17 | 2013-09-04 | 中国农业科学院农业资源与农业区划研究所 | Algae synergistic ammonium phosphate fertilizer and preparation method thereof |
| CN103749957A (en) * | 2013-12-31 | 2014-04-30 | 宜兴市天石饲料有限公司 | Preparation method for blue-green algae single-cell protein feed |
| CN103773721A (en) * | 2013-12-31 | 2014-05-07 | 陕西万源生物农业科技有限公司 | Composite probiotics for raising pigs |
| CN104962490A (en) * | 2015-06-09 | 2015-10-07 | 日照益康有机农业科技发展有限公司 | Enteromorpha microbial agent and preparation method thereof |
| CN105272420A (en) * | 2015-11-13 | 2016-01-27 | 云南云叶化肥股份有限公司 | Organic fertilizer containing blue algae and preparing method of organic fertilizer |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112299811A (en) * | 2020-01-09 | 2021-02-02 | 兰方环保工程(南京)有限公司 | Soil curing agent for road base and preparation method thereof |
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