CN106578522A - Preparation method of mixed-culture solid state fermentation piglet compound feed - Google Patents
Preparation method of mixed-culture solid state fermentation piglet compound feed Download PDFInfo
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Abstract
The invention discloses a preparation method of a mixed-culture solid state fermentation piglet compound feed, and relates to the technical field of mixed-culture feed fermentation. The preparation method comprises the following steps: (1) preparing a single-culture seed solvent: mixing a feed and water, sterilizing, inoculating a single culture respectively, and culturing in groups at the temperature of 25 to 30 DEG C for 4 to 6 hours to obtain a plurality of groups of single-culture seed solvents; (2) preparing a mixed-culture seed solvent: mixing the plurality of groups of single-culture seed solvents obtained in the step (1) to obtain the mixed-culture seed solvent; (3) performing mixed-culture solid fermentation: continually mixing the mixed-culture seed solvent prepared in the step (2) with the feed, adding water for humidifying, performing solid fermentation at the temperature of 25 to 30 DEG C for 24 to 36 hours to obtain the mixed-culture solid fermentation feed, vacuuming, packaging and storing. After the preparation method is used for producing the mixed-culture solid fermentation feed, the fermentation time can be effectively controlled within 2 days, the fermentation time is shortened greatly, and the production cost is lowered effectively; the preparation method is suitable for industrial production.
Description
Technical field
The present invention relates to mixed bacterium feed fermentation technical field, and in particular to a kind of mixed culture solid state fermentation compound feed for piglets
Preparation method.
Background technology
Piglet lacks because of innate immunity, the physiological property such as digestive system is unsound, occurs in that on the market many for son
The feed of pig, but some practical problems are often being occurred in that using on, such as feed intake reduction, growth retardation, diarrhoea increases
Situation.
In order to solve the above problems, primary raw material dregs of beans in baby pig feedstuff can be fermented with corn, can be degraded or portion
Divide the degraded wherein component such as antigen, albumen, starch, cellulose, resolve into small molecular protein, small peptide, compound sugar, monose, amino
The digestible composition such as acid, improves the abilities of digestive and absorption of piglet；Generate a large amount of regulation piglet stomaches in sweat simultaneously
Gut flora balances viable bacteria such as bacillus, lactic acid bacteria and saccharomycete etc., is conducive to controlling the intestinal health of piglet.
At present, because of low cost, fermented feed for piglets is based on solid state fermentation.Because single strain fermentation can only often solve certain
The problem of one side is restricted in use, is gradually substituted by mixed fungus fermentation.Mixed fungus fermentation is mixed with various inhomogeneities
The bacterial classification of type, its fermentation time is general all longer, usual more than 3 days, having or even longer.This inevitably increases labor
Fatigue resistance and production cost, while constraining the yield of product.
The content of the invention
It is an object of the present invention to provide a kind of preparation method of mixed culture solid state fermentation compound feed for piglets, to solve prior art
The high problem of fermentation time length, high labor intensive and production cost.
To solve the above problems, the technical scheme that the present invention is provided is：A kind of mixed culture solid state fermentation compound feed for piglets
Preparation method, comprises the steps：
(1) single bacterium seed liquor is prepared：Compound feed for piglets is mixed with water, being respectively connected to single culture after sterilizing again is carried out
Packet culture, cultivation temperature is 25-30 DEG C, and incubation time is 4-6 hours, and multigroup single bacterium seed liquor is obtained after the completion of culture；
(2) mixed bacterium seed liquor is prepared：Multigroup single bacterium seed liquor obtained in step (1) is mixed to get into mixed bacterium seed liquor；
(3) mixed culture solid state fermentation：Bacterium seed liquor will be mixed obtained in step (2) to continue to mix with feed, and the damping that adds water, enter
Row solid state fermentation, fermentation temperature is 25-30 DEG C, and fermentation time is 24-36 hours, and mixed culture solid state fermentation feeding is obtained after fermentation
Material, finally vacuumizes subpackage dress and preserves.
More excellent, in step (1), feed is 1 with the weight ratio of water:9.
It is more excellent, at least lactic acid bacteria, saccharomycete and bacillus subtilis in the single culture accessed in step (1).
More excellent, the single culture accessed in step (1) is lactic acid bacteria, saccharomycete and bacillus subtilis, and three kinds are single
Spawn incubation temperature, time and mode are respectively：Lactic acid bacteria：30 DEG C, quiescent culture 6 hours；Saccharomycete：25 DEG C, shaking table culture 4
Hour；Bacillus subtilis：25 DEG C, shaking table culture 4 hours.
More excellent, in step (2), three kinds of single bacterium seed liquors are mixed by following parts by volume：Lactic acid bacteria 1-3 parts；Saccharomycete 1-2
Part；Bacillus subtilis 1-2 parts.
More excellent, in step (3), mixed bacterium seed liquor is mixed by the following materials by weight with feed：Mixed bacterium seed liquor 1-10 part；Raise
Material 90-100 parts.
More excellent, in step (3), mix the damping that adds water after bacterium seed liquor mixes with feed and account for 35- to the weight ratio of moisture
45%.
The present invention has the advantage that compared with prior art：
(1) mixed culture solid state fermentation feed is produced using the inventive method, effectively fermentation time can be controlled within 2 days,
Fermentation time is substantially reduced, production cost is effectively reduced, is adapted to industrialized production.
(2) the mixed culture solid state fermentation feed nutrition that produced using the inventive method is abundant, greater homogeneity, stable performance, energy
Piglet is effectively improved to the digestion of feed, absorptivity and resistance against diseases, and is easy to store and is transported；
(3) after method of the present invention solid state fermentation, crude protein and small peptide are significantly improved, and crude fibre is degraded,
Content is substantially reduced, and product special flavour feature is substantially, and sour aromatic flavour can improve animal feed intake.
Specific embodiment
Embodiment 1
Piglet perfect compound feed is with water by weight 1:9 mixing, sterilizing, be respectively connected to various lactic acid bacterias, saccharomycete and
Bacteria suspension after the activation of bacillus subtilis parent species, the cultivation temperature of each bacterial classification, time and mode are：30 DEG C of lactic acid bacteria, stands
Culture 6 hours；25 DEG C of saccharomycete, shaking table culture 4 hours；Bacillus subtilis：25 DEG C, shaking table culture 4 hours.Each will be obtained
The seed liquor of bacterial classification presses volume mixture, and ratio is lactic acid bacteria:Saccharomycete:Bacillus subtilis=1:1:1, obtain mixed bacterium solid-state and send out
The seed liquor of kefir milk feed.
Prepared mixed bacterium seed liquor is mixed with complete feed for piglets, adds seed liquor to account for complete feed for piglets weight
1%, the damping that then adds water is to moisture 40% (weight ratio).Solid state fermentation 36 hours, temperature 25-30 DEG C, vacuum packaging,
Obtain mixed culture solid state fermentation feed.
Mixed culture solid state fermentation feed obtained in the present embodiment and the piglet perfect compound feed contrast situation fermented
Such as table 1：
The mixed culture solid state fermentation feed main component of table 1 and local flavor analysis contrast table
| Crude protein (%) | Crude fibre (%) | Small peptide (%) | Local flavor | |
| Complete feed for piglets (does not ferment) | 19.2 | 3.6 | 1.6 | Light fragrance |
| Mixed culture solid state fermentation feed | 24.5 | 1.2 | 4.8 | It is sour fragrant |
As it can be seen from table 1 in the present embodiment complete feed for piglets after method of the present invention solid state fermentation, crude protein
And small peptide is significantly improved, crude fibre is degraded, and content is substantially reduced, and product special flavour feature is substantially, sour aromatic flavour,
Animal feed intake can be improved, and fermentation time is short, reduces production cost.
Embodiment 2
Piglet perfect compound feed is with water by weight 1:9 mixing, sterilizing, be respectively connected to various lactic acid bacterias, saccharomycete and
Bacteria suspension after the activation of bacillus subtilis parent species, the cultivation temperature of each bacterial classification, time and mode are：30 DEG C of lactic acid bacteria, stands
Culture 6 hours；25 DEG C of saccharomycete, shaking table culture 4 hours；Bacillus subtilis：25 DEG C, shaking table culture 4 hours.Each will be obtained
The seed liquor of bacterial classification presses volume mixture, and ratio is lactic acid bacteria:Saccharomycete:Bacillus subtilis=3:2:2, obtain mixed bacterium solid-state and send out
The seed liquor of kefir milk feed.
Prepared mixed bacterium seed liquor is mixed with complete feed for piglets, adds seed liquor to account for complete feed for piglets weight
10%, the damping that then adds water is to moisture 40% (weight ratio).Solid state fermentation 24 hours, temperature 25-30 DEG C, vacuum packet
Dress, that is, obtain mixed culture solid state fermentation feed.
Mixed culture solid state fermentation feed obtained in the present embodiment and the piglet perfect compound feed contrast situation fermented
Such as table 2：
The mixed culture solid state fermentation feed main component of table 2 and local flavor analysis contrast table
| Crude protein (%) | Crude fibre (%) | Small peptide (%) | Local flavor | |
| Complete feed for piglets (does not ferment) | 19.2 | 3.6 | 1.6 | Light fragrance |
| Mixed culture solid state fermentation feed | 26.8 | 1.0 | 5.9 | It is sour fragrant |
From table 2 it can be seen that in the present embodiment complete feed for piglets after method of the present invention solid state fermentation, crude protein
And small peptide is significantly improved, crude fibre is degraded, and content is substantially reduced, and product special flavour feature is substantially, sour aromatic flavour,
Animal feed intake can be improved, and fermentation time is short, reduces production cost.
Embodiment 3
The process of the test of product of the present invention：
Piglet perfect compound feed, the piglet full price are selected according to current Chinese feeding standard (NY/T 65-2004)
Mixed feed mainly includes corn, dregs of beans, wheat, rice bran meal, stone flour, calcium monohydrogen phosphate, threonine, lysine, salt etc., its
Composition is shown in Table shown in 3, table 4 with trophic level：
The piglet perfect compound feed of table 3 constitutes (air bells dry basis)
| Project | Content (percentage by weight) |
| Corn | 67.74 |
| Dregs of beans (46%) | 17.83 |
| Wheat bran | 3.9 |
| Wheat-middlings | 1.52 |
| Fish meal | 3.2 |
| Lysine (98%) | 0.42 |
| Methionine (99%) | 0.2 |
| Threonine (98.5%) | 0.19 |
| Soya-bean oil | 1.00 |
| Premix | 4.00 |
| It is total | 100.00 |
The trophic level (dry matter basis) of the piglet perfect compound feed of table 4
| Project | Content |
| Thick protein (%) | 17.06 |
| Lysine (%) | 1.17 |
| Methionine (%) | 0.46 |
| Methionine+cysteine (%) | 0.67 |
| Threonine (%) | 0.88 |
| Calcium (%) | 0.69 |
| Total phosphorus (%) | 0.64 |
| Available phosphorus (%) | 0.37 |
| Crude fat (%) | 4.14 |
| Digestible energy (MJ/kg) | 14.00 |
Wherein, premix is made up of copper, zinc, iron, manganese, iodine and commercially available vitamin etc., and concrete every kilogram of diet is provided：
FeSO4·7H2O 500mg, ZnSO4·7H2O 60mg, CuSO4·5H2O 19.9mg, MgSO310mg, KI 0.22mg,
MnSO4·H2O 10.3mg, VA 5 400IU, VD3110IU, VB12.1mg, VB215mg, VB120.04mg, VE 18IU,
Citric acid 12mg, carnitine 0.5mg, antioxidant 5mg, mould inhibitor 12.1mg, chromium picolinate 5mg, calcium dihydrogen phosphate Ca
(H2PO4)2285mg, stone flour 300mg, carrier is zeolite powder.
Test is randomly divided into 2 groups from the DLY piglet 160 of body weight 10.20kg ± 0.10kg：Control group and test
Group, per group of 8 repetitions, control group ration ingredient is the piglet perfect compound feed shown in table 3, and test group daily ration is according to this
The mixed culture solid state fermentation feed obtained after piglet perfect compound feed shown in the method fermentation table 3 of invention, test period is 30
My god.
Testing index and method：
ADFI=(feeding doses-surplus doses)/test number of days；
ADG=(last weight-starting weight)/test number of days；
The average day Bian appetite/average daily gains of F/G=；
The diarrhea rate %=grice diarrhoeas head number/total head number * 100 of piglet.
Data process＆analysis carry out variance analysis, multiple ratio using the one-way ANOVA programs of the softwares of SPSS 13.0
Compared with；Using Duncan methods, with P<0.05, as significance of difference criterion, is as a result represented with " mean value ± standard error ", is obtained
The concrete data for arriving are as shown in table 5：
The mixed culture solid state fermentation feed of table 5 uses tables of data
| Project | Control group | Test group | P values |
| Begin /kg | 10.11±0.30 | 10.26±0.26 | 0.598 |
| Last counterpoise/kg | 22.06±0.67 | 26.87±0.91 | 0.091 |
| Average daily gain ADFI/ (kg/d) | 0.65±0.03 | 0.73±0.06 | 0.220 |
| Average daily gain ADG/ (kg/d) | 0.36±0.03 | 0.46±0.04 | 0.097 |
| Feed-weight ratio F/G | 1.80±0.07a | 1.58±0.08b | 0.049 |
| Diarrhea rate % | 18.00±2.0A | 7.00±0.8B | ＜ 0.001 |
| IgA(g/L) | 1.20±0.01 | 1.26±0.01 | 0.721 |
| IgG(g/L) | 6.01±0.25b | 7.23±0.30a | 0.030 |
| IgM(g/L) | 0.78±0.05b | 1.05±0.07a | 0.011 |
Note：Colleague is without letter or represents difference not significantly (P ＞ 0.05), different lowercase letter indication differences significantly (P ＜
0.05), different capitalizations represent difference extremely significantly (P ＜ 0.01).
As a result show, mixed culture solid state fermentation feed has the trend (0.05 ＜ P ＜ 0.10) for increasing piglet daily gain, can be significantly
Piglet feedstuff-meat ratio (P ＜ 0.05) is reduced, pole significantly reduces grice diarrhoea rate (P ＜ 0.01), also can significantly improve in piglet serum
IgG and IgM contents (P ＜ 0.05), improve piglet resistance, fully proves the present invention provide method preparation mixed bacterium solid-state
Fermented feed can effectively improve piglet to the digestion of feed, absorptivity and resistance against diseases.
Embodiment described above only expresses the specific embodiment of the application, and its description is more concrete and detailed, but and
Therefore the restriction to the application protection domain can not be interpreted as.It should be pointed out that for one of ordinary skill in the art
For, on the premise of conceiving without departing from technical scheme, some deformations and improvement can also be made, these belong to this
The protection domain of application.
Claims (7)
1. a kind of preparation method of mixed culture solid state fermentation compound feed for piglets, it is characterised in that comprise the steps：
(1) single bacterium seed liquor is prepared：Compound feed for piglets is mixed with water, single culture is respectively connected to after sterilizing again and is grouped
Culture, cultivation temperature is 25-30 DEG C, and incubation time is 4-6 hours, and multigroup single bacterium seed liquor is obtained after the completion of culture；
(2) mixed bacterium seed liquor is prepared：Multigroup single bacterium seed liquor obtained in step (1) is mixed to get into mixed bacterium seed liquor；
(3) mixed culture solid state fermentation：Bacterium seed liquor will be mixed obtained in step (2) to continue to mix with feed, and the damping that adds water, carry out solid
State is fermented, and fermentation temperature is 25-30 DEG C, and fermentation time is 24-36 hours, mixed culture solid state fermentation feed is obtained after fermentation, most
After vacuumize subpackage dress preserve.
2. a kind of preparation method of mixed culture solid state fermentation compound feed for piglets according to claim 1, it is characterised in that step
Suddenly in (1), feed is 1 with the weight ratio of water:9.
3. a kind of preparation method of mixed culture solid state fermentation compound feed for piglets according to claim 1 and 2, its feature exists
At least lactic acid bacteria, saccharomycete and bacillus subtilis in the single culture accessed in, step (1).
4. a kind of preparation method of mixed culture solid state fermentation compound feed for piglets according to claim 3, it is characterised in that step
Suddenly the single culture for accessing in (1) is lactic acid bacteria, saccharomycete and bacillus subtilis, three kinds of single culture cultivation temperatures, times
And mode is respectively：
Lactic acid bacteria：30 DEG C, quiescent culture 6 hours；
Saccharomycete：25 DEG C, shaking table culture 4 hours；
Bacillus subtilis：25 DEG C, shaking table culture 4 hours.
5. a kind of preparation method of mixed culture solid state fermentation compound feed for piglets according to claim 4, it is characterised in that step
Suddenly in (2), three kinds of single bacterium seed liquors are mixed by following parts by volume：
Lactic acid bacteria 1-3 parts；
Saccharomycete 1-2 parts；
Bacillus subtilis 1-2 parts.
6. a kind of preparation method of mixed culture solid state fermentation compound feed for piglets according to claim 5, it is characterised in that step
Suddenly in (3), mixed bacterium seed liquor is mixed by the following materials by weight with feed：
Mixed bacterium seed liquor 1-10 part；
Feed 90-100 parts.
7. a kind of preparation method of mixed culture solid state fermentation compound feed for piglets according to claim 6, it is characterised in that step
Suddenly in (3), mix the damping that adds water after bacterium seed liquor mixes with feed and account for 35-45% to the weight ratio of moisture.
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| CN108902496A (en) * | 2018-07-04 | 2018-11-30 | 象山互易科技咨询有限公司 | A kind of preparation method and applications of feed |
| CN109329569A (en) * | 2018-12-05 | 2019-02-15 | 湖南赛福资源饲料科技有限公司 | Fermented feed preprocessing method of raw materials |
| CN110870511A (en) * | 2019-10-24 | 2020-03-10 | 辽宁波尔莱特农牧实业有限公司 | Dry-wet mixed concentrated feed and preparation method thereof |
| CN111466481A (en) * | 2020-04-13 | 2020-07-31 | 博益德（北京）生物科技有限公司 | Liquid bacterium solution for fermenting liquid piglet feed, preparation method and application of liquid fermented piglet feed |
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| CN110870511A (en) * | 2019-10-24 | 2020-03-10 | 辽宁波尔莱特农牧实业有限公司 | Dry-wet mixed concentrated feed and preparation method thereof |
| CN111466481A (en) * | 2020-04-13 | 2020-07-31 | 博益德（北京）生物科技有限公司 | Liquid bacterium solution for fermenting liquid piglet feed, preparation method and application of liquid fermented piglet feed |
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Application publication date: 20170426 |