CN107897368A - A kind of probiotics fermention breast and preparation method thereof - Google Patents
A kind of probiotics fermention breast and preparation method thereof Download PDFInfo
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- CN107897368A CN107897368A CN201711129306.7A CN201711129306A CN107897368A CN 107897368 A CN107897368 A CN 107897368A CN 201711129306 A CN201711129306 A CN 201711129306A CN 107897368 A CN107897368 A CN 107897368A
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- lactobacillus plantarum
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/13—Fermented milk preparations; Treatment using microorganisms or enzymes using additives
- A23C9/1307—Milk products or derivatives; Fruit or vegetable juices; Sugars, sugar alcohols, sweeteners; Oligosaccharides; Organic acids or salts thereof or acidifying agents; Flavours, dyes or pigments; Inert or aerosol gases; Carbonation methods
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/13—Fermented milk preparations; Treatment using microorganisms or enzymes using additives
- A23C9/1322—Inorganic compounds; Minerals, including organic salts thereof, oligo-elements; Amino-acids, peptides, protein-hydrolysates or derivatives; Nucleic acids or derivatives; Yeast extract or autolysate; Vitamins; Antibiotics; Bacteriocins
Abstract
The invention discloses a kind of probiotics fermention breast, which is made of the raw material of following mass percent：Leavening 0.001% 0.006%, lactobacillus plantarum ST III 0.001% 0.006%, lactobacillus plantarum ST III multiplication agents 16% 33%, stabilizer 0.25% 0.5%, sweetener 1% 8%, surplus are that the sum of raw milk, each component mass percent is 100%.Raw milk, lactobacillus plantarum ST III multiplication agents, sweetener and stabilizer are mixed first, stirred evenly, homogeneous, thermal sterilization processing, cooling；Leavening and lactobacillus plantarum ST III are seeded in the mixture after processing and carry out fermentation process, acidity value reaches 70 85oFermentation is terminated during T, turns over cylinder cooling, up to the probiotics fermention breast.Probiotics fermention breast, which has, adjusts intestinal flora balance, improves immunity of organisms function.There is reducing blood lipid, norcholesterol in terms of angiocardiopathy.
Description
Technical field
The present invention relates to dairy fermentation technical field, and in particular to a kind of probiotics fermention breast and preparation method thereof, more
Body is related to a kind of acidified milk containing high vigor high-content lactobacillus plantarum ST-III and prepares.
Background technology
Probiotics fermention breast is also metabolized the feature thing of generation while cow's milk nutrition composition is retained containing profitable probliotics
Matter, just plays an important role human body intestinal canal health and colony balance, and abundant probiotics fermention milk product is of great significance.It is commercially available
In probiotics fermention milk product, the probiotics used more has animal bifidobacteria Bb-12, lactobacillus acidophilus NCFMs and cheese breast
Bacillus Shirota etc., these probiotic strains all are from foreign study mechanism or enterprise, therefore to being produced with China's independent intellectual
Weigh bacterial strain and implement extensive commercial application, there is important strategic importance.
Lactobacillus plantarum ST-III has human body a huge prebiotic effect, Chinese invention patent ZL03116377.7 and
ZL200410066891.7 makes public for the first time lactobacillus plantarum ST-III bacterial strains and its adjusts the application of lipid aspects, and having reduces
The effect of serum cholesterol and raising high-density lipoprotein cholesterol content, have control or lose weight, auxiliary weight-reducing, it is seen that
Lactobacillus plantarum ST-III has human body many benefits, while lactobacillus plantarum ST-III full-length genomes were surveyed in 2010
It is fixed, and be used widely in actual production.However, the study found that lactobacillus plantarum ST-III is added to the newborn base such as cow's milk
Cultivated in matter, the biomass of acquisition is very low, and almost without growth, reason may be missing from utilizing relevant peptide with micromolecule polypeptide
Enzyme activity, can not independently synthesize some required nutriments using cow's milk.Enterprise solves the problems, such as that this method is directly to throw at present
Bacterium powder is put, which is to be prepared after lactobacillus plantarum ST-III zymotic fluids are concentrated by vacuum freeze drying, and advantage is
Bacteria concentration is high, energetic, and shortcoming is the of high cost of the equipment requirement of demand, technological means and cost.
A kind of safe source is found, is prepared simply, cheap promotion lactobacillus plantarum ST-III growth substances are very
Necessity, is added in cow's milk and promotes the growth and breeding of ST-III, fermented and be prepared into containing the high viable count ST- of high vigor
The probiotics fermention breast of III.
The content of the invention
The technical problem to be solved by the invention is to provide a kind of probiotics fermention breast.Probiotics fermention breast contains height
The lactobacillus plantarum ST-III of the high viable count of vigor.Lactobacillus plantarum ST-III has as probiotics in itself adjusts intestinal flora
Balance, improves immunity of organisms function, but because lactobacillus plantarum ST-III is difficult to be bred in raw milk, causes plant newborn
The product of bacillus ST-III fermentations has effects that reducing blood lipid, norcholesterol are difficult to be applied to acidified milk in terms of angiocardiopathy
Field.The present invention solves this technical problem, makes originally in raw milk by adding lactobacillus plantarum ST-III multiplication agents
It is difficult to the lactobacillus plantarum ST-III of breeding is largely bred, so that reach the activity of enhancing lactobacillus plantarum ST-III, increase
The effect of acidified milk functional application.
To achieve the above object, the present invention adopts the following technical scheme that：
A kind of probiotics fermention breast, the acidified milk are made of the raw material of following mass percent：Leavening 0.001%-
0.006%, lactobacillus plantarum ST-III 0.001%-0.006%, lactobacillus plantarum ST-III multiplication agent 16%-33%, stablize
Agent 0.25%-0.5%, sweetener 1%-8%, surplus are that the sum of raw milk, each component mass percent is 100%.
Further optimization, the lactobacillus plantarum ST-III multiplication agents are the fermentations of series bacillus CGMCC No.8333
One or more in supernatant, leucine, isoleucine, valine, tyrosine, methionine or phenylalanine, wherein class bud
The fermented supernatant fluid additive amount of spore bacillus CGMCC No.8333 is 15%-30%, and a variety of amino acid additive amounts are 1%-
3%.Experiment shows that the micromolecule polypeptide class in the fermented supernatant fluid or amino acid of series bacillus CGMCC No.8333 is produced
Thing and relevant peptase can make to be difficult the lactobacillus plantarum ST-III bred substantial amounts of numerous in raw milk in raw milk originally
Grow, so as to reach the activity of enhancing lactobacillus plantarum ST-III.By prolific lactobacillus plantarum ST-III conducts in itself
Probiotics, which has, adjusts intestinal flora balance, improves immunity of organisms function, and prolific lactobacillus plantarum ST-III
The tunning of generation has effects that reducing blood lipid, norcholesterol so that lactobacillus plantarum in terms of angiocardiopathy is treated
The activity of ST-III greatly enhances, so as to improve the function affect of the acidified milk.
Further optimization, the raw milk are fresh milk or processing breast.
Further optimization, the lactobacillus plantarum ST-III additive amounts are 106-108CFU/mL。
Further optimization, the leavening are lactobacillus bulgaricus, streptococcus thermophilus, Lactobacillus casei, newborn bifid bar
One or more in bacterium or Lactobacillus rhamnosus；The leavening additive amount is 106-108CFU/mL。
Further optimize, further included in the acidified milk in fruit juice, jam, essence, chocolate, coffee or mouthfeel modifying agent
One or more, content 1%-10%.
Further optimize, one kind in oligofructose, galactooligosaccharide, inulin or poly- glucose sugar is further included in the acidified milk
Or several, content 1%-1.5%.
A kind of method for preparing probiotics fermention breast, this method comprise the following steps：
(1) raw milk, lactobacillus plantarum ST-III multiplication agents, sweetener and stabilizer are mixed, stirred evenly, homogeneous,
Thermal sterilization processing, cooling；
(2) leavening and lactobacillus plantarum ST-III are seeded in the mixture after step (1) processing and carried out at fermentation
Reason, acidity value terminate fermentation when reaching 70-85 ° of T；Cylinder cooling is turned over, turns over cylinder cooling temperature as 15-25 DEG C, up to described prebiotic
Bacterium acidified milk.
Further optimization, temperature when step (1) raw milk, multiplication agent, sweetener and stabilizer mix is 45-55
DEG C, mixing time 5-15min；The homogenizing temperature is 55-65 DEG C, and level-one homogenization pressure is 15-20MPa, double-stage homogenization pressure
Power is 2-5MPa；The sterilization conditions are 85-95 DEG C of temperature, time 10-25min；The cooling temperature is 38-45 DEG C.
Further optimize the scheme of acidified milk
A kind of probiotics fermention breast, is made of the raw material of following mass percent：Leavening, lactobacillus plantarum ST-III,
Lactobacillus plantarum ST-III multiplication agents, stabilizer, sweetener, surplus are that the sum of raw milk, each component mass percent is
100%.
In the present invention, the raw milk is raw milk commonly used in the art, preferably fresh milk and processing breast；Institute
State fresh milk and be preferably raw milk；The processing breast is preferably reconstituted milk；The reconstituted milk is this area routine operation
Prepared by method, by milk powder and water after 35-45 DEG C is mixed, 8 DEG C of refrigerations are spare.
The stabilizer is this area conventional stabilizer, preferably pectin, carragheen, gelatin, agar, gellan gum, sea
One or more in propylene glycol alginate and converted starch are more preferably pectin, one kind in gelatin, agar and converted starch
It is or a variety of.The stabiliser content is preferably 0.25%-0.5%, is more preferably 0.35%-0.45%, most preferably for
0.4%, the percentage is the mass percent for accounting for the raw material gross mass.
The sweetener is this area conventional sweetener, preferably white granulated sugar, Aspartame, acesulfame potassium, fructose, fruit Portugal
One or more in syrup, xylitol and Sucralose are more preferably white granulated sugar, in Aspartame, acesulfame potassium and fructose syrup
One or more, be most preferably white granulated sugar, Aspartame and acesulfame potassium.When the sweetener is white granulated sugar, content is preferable
Ground is 1%-8%, is more preferably 3%-8%, is most preferably 5%-7%；When the sweetener is Aspartame and acesulfame potassium,
Content is preferably 0.01%-0.03%, is most preferably 0.02%, and the percentage is the quality hundred for accounting for the raw material gross mass
Divide ratio.
The leavening is this area normal fermentation agent, preferably lactobacillus bulgaricus, streptococcus thermophilus, cheese breast
One or more in bacillus, bifidobacterium lactis and Lactobacillus rhamnosus；More preferably it is lactobacillus bulgaricus and/or thermophilic chain
Coccus.The leavening additive amount is preferably 106-108CFU/mL, is more preferably 106-107CFU/mL, most preferably for
107CFU/mL。
The lactobacillus plantarum ST-III is this area routine bacterial strain.The additive amount is preferably 106-108CFU/
ML, is more preferably 106-107CFU/mL, is most preferably 107CFU/mL。
The lactobacillus plantarum ST-III multiplication agents can be series bacillus CGMCCNo.8333 fermented supernatant fluids, ammonia
One or more in base acid.The series bacillus CGMCC No.8333 fermented supernatant fluid additive amounts are preferably 15%-
30%, more it is 20%-25%, is most preferably 25%.The amino acid is preferably leucine, isoleucine, figured silk fabrics ammonia
One or more in acid, tyrosine, methionine and phenylalanine are more preferably leucine, in isoleucine and valine
It is one or more.The amino acid additive amount is preferably 1%-3%, is more preferably 1.5%-2.5%, optimal for 2%,
The percentage is the mass percent for accounting for the raw material gross mass.
The water is this area routine drinking water.
The invention also discloses the preparation method of above-mentioned probiotics fermention breast, including raw milk pretreatment, homogeneous, heat are killed
Bacterium, cooling, inoculation fermentation, turn over cylinder cooling, sterile filling.
More specifically, include the following steps：
(1) raw milk, lactobacillus plantarum ST-III multiplication agents, sweetener and stabilizer are mixed first, stirred evenly,
Matter, thermal sterilization processing, cooling；
(2) leavening and lactobacillus plantarum ST-III are seeded in the mixture after step (1) processing and carried out at fermentation
Reason.Acidity value terminates fermentation when reaching 70-85 ° of T, turns over cylinder cooling, up to probiotics fermention of the present invention breast.
Wherein, temperature when step (1) raw milk, multiplication agent, sweetener and stabilizer mix preferably is maintained at 45-
55℃；And/or mixing time is 5-15min；The homogenizing temperature is preferably maintained at 55-65 DEG C, and level-one homogenization pressure is preferably protected
Hold and be preferably maintained at 2-5MPa in 15-20MPa, double-stage homogenization pressure；The sterilization conditions are preferably 85-95 DEG C of temperature, time
10-25min；The cooling temperature is preferably maintained at 38-45 DEG C.
More preferably, the temperature when raw milk, multiplication agent, sweetener and stabilizer mix is 50-55 DEG C；Mixing time
For 10-15min.
The homogenizing temperature is 60 DEG C, and level-one homogenization pressure is 18MPa, double-stage homogenization pressure 3MPa.
The thermally-sterilized condition：Pasteurizing temperature is 90-95 DEG C, time 5-10min.
Step (2) fermentation process condition is preferably：Fermentation time is 5-24h, and fermentation temperature is 35-45 DEG C.More preferably, institute
It is 6-16h to state the fermentation process time, and fermentation temperature is 40-45 DEG C.Most preferably fermentation temperature is 42 DEG C.The terminal fermentation acid
Angle value is preferably 70-85 ° of T, is more preferably 70-80 ° of T, is most preferably 75 ° of T.Final fermentation time is determined by fermentation acidity.
Turn over cylinder cooling temperature and be preferably 15-25 DEG C, be more preferably 16-23 DEG C, be most preferably 20 DEG C.
In the present invention, raw material can also preferably include various flavor substances.The flavor substance is conventional for this area
One or more in material, preferably fruit juice, jam, essence, chocolate, coffee and mouthfeel modifying agent.The flavor substances
Matter content is preferably 1%-10%, is more preferably 1%-8%, and the percentage is the quality percentage for accounting for the raw material gross mass
Than.
In the present invention, raw material can also preferably include prebiotics, the prebiotics in step (1) with the raw material
Breast, multiplication agent, stabilizer and sweetener add together.The prebiotics is this area conventional substances, preferably oligomeric fruit
One or more in sugar, galactooligosaccharide, inulin and poly- glucose sugar.The prebiotics content is preferably 1%-1.5%, more
It is goodly 1%, the percentage is the mass percent for accounting for the raw material gross mass.
On the basis of common knowledge of the art, above-mentioned each optimum condition, can be combined, up to each good reality of the present invention
Example.
Above-mentioned probiotics fermention breast can be prepared into various fermented dairy products according to actual conditions, every to utilize the present invention's
Dairy products prepared by probiotics fermention breast, are within the scope of the present invention.
Beneficial effects of the present invention are：
Probiotics fermention breast prepared by the present invention, which has, adjusts intestinal flora balance, improves immunity of organisms function.In the heart
There is reducing blood lipid, norcholesterol in terms of vascular diseases；
Compared with customary preparation methods, probiotics fermention breast preparation method provided by the invention is simple, and equipment requirement is low, drop
Low production cost, has potential application value.
Embodiment
The present invention will be further explained by specific embodiment below, it being understood, however, that can be with each
Kind form realizes the present invention without should be limited by embodiments set forth here.Conversely, there is provided these embodiments are to be able to
The present invention is best understood from, and the scope of the present invention can be completely communicated to those skilled in the art.
"comprising" or " comprising " as mentioned in working as in specification in the whole text and claim are an open language, therefore should
It is construed to " including but not limited to ".To implement the better embodiment of the present invention, so description is specification subsequent descriptions
For the purpose of the rule of specification, the scope of the present invention is not limited to.Protection scope of the present invention is when regarding appended power
Profit is required subject to institute's defender.
There is no specified otherwise such as, various raw materials of the present invention are commercially available.
Embodiment 1
Prepared by one Bacillus species CGMCC No.8333 fermented supernatant fluids, specific as follows：
(1) activate：Series bacillus CGMCC No.8333 are inoculated in 50ml TYC culture mediums, temperature for 25 DEG C,
Shaking speed cultivates 24h as seed liquor under conditions of being 200rpm/min；
(2) spread cultivation：Seed liquor is inoculated in 500mL mass percents according to the inoculum concentration that percent by volume is 3% (V/V)
For in 10% degreasing milk medium, culture 120h is obtained pure under conditions of temperature is 25 DEG C, shaking speed is 200rpm/min
Culture；
(3) centrifuge：Pure culture obtained by step (2) is centrifuged under the conditions of temperature is 10 DEG C, rotating speed is 8000rpm/min
10min, takes supernatant up to the lactobacillus plantarum ST-III multiplication agents.
Embodiment 2
Probiotics fermention dairy milk starting material formula：Leavening 108CFU/mL, lactobacillus plantarum ST-III 108CFU/mL, class gemma
Bacillus CGMCC No.8333 fermented supernatant fluids 30%, pectin 0.5%, acesulfame potassium 0.02%, surplus are raw milk, each group sub-prime
It is 100% to measure the sum of percentage.
The preparation method of above-mentioned probiotics fermention breast is：
(1) by fresh milk, pectin, acesulfame potassium and series bacillus CGMCC No.8333 fermented supernatant fluids in 55 DEG C of conditions
Under, 5min is sufficiently stirred, is uniformly mixed, 15MPa level-ones homogeneous at 65 DEG C, 5MPa double-stage homogenizations.Under the conditions of 95 DEG C, thermal sterilization
10min is handled, is cooled to 45 DEG C.
(2) by leavening lactobacillus bulgaricus 108CFU/mL, streptococcus thermophilus 108CFU/mL and lactobacillus plantarum ST-
III 108CFU/mL is seeded in the cow's milk after step (1) processing to ferment under the conditions of 45 DEG C, after fermentation acidity reaches 70 ° of T,
Terminate fermentation.Turn over cylinder and be cooled to 15 DEG C, sterile filling is up to probiotics fermention of the present invention breast.
Embodiment 3
Probiotics fermention dairy milk starting material formula：Leavening 5 × 106CFU/mL, lactobacillus plantarum ST-III107CFU/mL, class bud
Spore bacillus CGMCC No.8333 fermented supernatant fluids 25%, converted starch 0.4%, white granulated sugar 6%, surplus are fresh milk, each component
The sum of mass percent is 100%.
The preparation method of above-mentioned probiotics fermention breast is：
(1) by fresh milk, converted starch, white granulated sugar and series bacillus CGMCC No.8333 fermented supernatant fluids in 50 DEG C
Under the conditions of, 10min is sufficiently stirred, is uniformly mixed, 18MPa level-ones homogeneous at 60 DEG C, 4MPa double-stage homogenizations.Under the conditions of 90 DEG C,
Thermal sterilization handles 20min, is cooled to 40 DEG C.
(2) by leavening lactobacillus bulgaricus 5 × 106CFU/mL, streptococcus thermophilus 5 × 106CFU/mL and plant breast bar
Bacterium ST-III 107CFU/mL is seeded in the cow's milk after step (1) processing to ferment under the conditions of 40 DEG C, and fermentation acidity reaches 75 °
After T, fermentation is terminated.Turn over cylinder and be cooled to 20 DEG C, sterile filling is up to probiotics fermention of the present invention breast.
Embodiment 4
Probiotics fermention dairy milk starting material formula：
Leavening 106CFU/mL, lactobacillus plantarum ST-III 106CFU/mL, valine 1%, gellan gum 0.25%, A Si
Ba Tian 0.03%, surplus are that the sum of reconstituted milk, each component mass percent is 100%.
The preparation method of above-mentioned probiotics fermention breast is：
(1) by reconstituted milk, gellan gum, Aspartame and valine under the conditions of 45 DEG C, 15min is sufficiently stirred, mixing is equal
It is even, 20MPa level-ones homogeneous at 55 DEG C, 2MPa double-stage homogenizations.Under the conditions of 85 DEG C, thermal sterilization processing 25min, is cooled to 38 DEG C.
(2) by leavening lactobacillus bulgaricus 108CFU/mL, streptococcus thermophilus 108CFU/mL and lactobacillus plantarum ST-
III 108CFU/mL is seeded in the cow's milk after step (1) processing to ferment under the conditions of 38 DEG C, after fermentation acidity reaches 85 ° of T,
Terminate fermentation.Turn over cylinder and be cooled to 25 DEG C, sterile filling is up to probiotics fermention of the present invention breast.
Embodiment 5
Probiotics fermention dairy milk starting material formula：Leavening 8 × 106CFU/mL, lactobacillus plantarum ST-III 3 × 107CFU/mL,
Leucine and valine 3%, gelatin and propylene glycol alginate 0.35%, fructose 5%, surplus are reconstituted milk, each component quality
The sum of percentage is 100%.
The preparation method of above-mentioned probiotics fermention breast is：
(1) by reconstituted milk, gelatin, propylene glycol alginate, fructose, leucine and valine under the conditions of 55 DEG C, fully
5min is stirred, is uniformly mixed, 15MPa level-ones homogeneous at 65 DEG C, 4MPa double-stage homogenizations.Under the conditions of 85 DEG C, thermal sterilization processing
25min, is cooled to 42 DEG C.
(2) by leavening lactobacillus bulgaricus 8 × 106CFU/mL, streptococcus thermophilus 8 × 106CFU/mL and plant breast bar
Bacterium ST-III 3 × 107CFU/mL is seeded in the cow's milk after step (1) processing to ferment under the conditions of 42 DEG C, and fermentation acidity reaches
After 71 ° of T, fermentation is terminated.Turn over cylinder and be cooled to 22 DEG C, sterile filling is up to probiotics fermention of the present invention breast.
Embodiment 6
Probiotics fermention dairy milk starting material formula：Leavening 3 × 107CFU/mL, lactobacillus plantarum ST-III 4 × 107CFU/mL,
Leucine 2%, series bacillus CGMCC No.8333 fermented supernatant fluids 20%, mango essence 1%, carragheen and gelatin
0.45%, fructose syrup 8%, surplus is that the sum of fresh milk, each component mass percent is 100%.
The preparation method of above-mentioned probiotics fermention breast is：
(1) by fresh milk, carragheen, gelatin, fructose syrup, mango essence, leucine and series bacillus CGMCC
No.8333 fermented supernatant fluids are sufficiently stirred 10min under the conditions of 55 DEG C, are uniformly mixed, 17MPa level-ones homogeneous, 4MPa at 60 DEG C
Double-stage homogenization.Under the conditions of 92 DEG C, thermal sterilization processing 10min, is cooled to 40 DEG C.
(2) by leavening lactobacillus bulgaricus 3 × 107CFU/mL, streptococcus thermophilus 3 × 107CFU/mL and plant breast bar
Bacterium ST-III4 × 107CFU/mL is seeded in the cow's milk after step (1) processing to ferment under the conditions of 40 DEG C, and fermentation acidity reaches
After 80 ° of T, fermentation is terminated.Turn over cylinder and be cooled to 24 DEG C, sterile filling is up to probiotics fermention of the present invention breast.
Embodiment 7
Probiotics fermention dairy milk starting material formula：Leavening 5 × 107CFU/mL, lactobacillus plantarum ST-III107CFU/mL, it is different bright
Propylhomoserin 2.5%, converted starch and pectin 0.42%, strawberry jam 1%, Sucralose 0.01%, surplus are reconstituted milk, each group sub-prime
It is 100% to measure the sum of percentage.
The preparation method of above-mentioned probiotics fermention breast is：
(1) by reconstituted milk, converted starch, pectin, acesulfame potassium, strawberry jam and isoleucine under the conditions of 50 DEG C, fully
10min is stirred, is uniformly mixed, 16MPa level-ones homogeneous at 55 DEG C, 5MPa double-stage homogenizations.Under the conditions of 90 DEG C, thermal sterilization processing
13min, is cooled to 39 DEG C.
(2) by leavening lactobacillus bulgaricus 5 × 107CFU/mL, streptococcus thermophilus 5 × 107CFU/mL and plant breast bar
Bacterium ST-III 107CFU/mL is seeded in the cow's milk after step (1) processing to ferment under the conditions of 39 DEG C, and fermentation acidity reaches 74 °
After T, fermentation is terminated.Turn over cylinder and be cooled to 25 DEG C, sterile filling is up to probiotics fermention of the present invention breast.
Embodiment 8
Probiotics fermention dairy milk starting material formula：Leavening 9 × 106CFU/mL, lactobacillus plantarum ST-III107CFU/mL, it is oligomeric
Fructose and galactooligosaccharide 1.5%, leucine 2.5%, agar and gellan gum 0.45%, white granulated sugar 8%, acesulfame potassium 0.03%,
Surplus is that the sum of fresh milk, each component mass percent is 100%.
The preparation method of above-mentioned probiotics fermention breast is：
(1) by fresh milk, agar, gellan gum, white granulated sugar, acesulfame potassium, oligofructose, galactooligosaccharide and leucine in 45
Under the conditions of DEG C, 10min is sufficiently stirred, is uniformly mixed, 19MPa level-ones homogeneous at 55 DEG C, 2MPa double-stage homogenizations.In 90 DEG C of conditions
Under, thermal sterilization processing 10min, is cooled to 42 DEG C.
(2) by leavening lactobacillus bulgaricus 9 × 106CFU/mL, streptococcus thermophilus 9 × 106CFU/mL and plant breast bar
Bacterium ST-III 107CFU/mL is seeded in the cow's milk after step (1) processing to ferment under the conditions of 42 DEG C, and fermentation acidity reaches 80 °
After T, fermentation is terminated.Turn over cylinder and be cooled to 18 DEG C, sterile filling is up to probiotics fermention of the present invention breast.
Embodiment 9
Probiotics fermention dairy milk starting material formula：Leavening 107CFU/mL, lactobacillus plantarum ST-III107CFU/mL, inulin and
Portugal's Fructooligosaccharides 1.2%, tyrosine and phenylalanine 2%, propylene glycol alginate and pectin 0.38%, acesulfame potassium and Aspartame
0.02%, surplus is that the sum of reconstituted milk, each component mass percent is 100%.
The preparation method of above-mentioned probiotics fermention breast is：
(1) by reconstituted milk, propylene glycol alginate, pectin, acesulfame potassium, Aspartame, inulin, Portugal's Fructooligosaccharides, tyrosine
With phenylalanine under the conditions of 50 DEG C, 15min is sufficiently stirred, is uniformly mixed, 18MPa level-ones homogeneous at 60 DEG C, 3MPa two levels are equal
Matter.Under the conditions of 92 DEG C, thermal sterilization processing 10min, is cooled to 42 DEG C.
(2) by leavening lactobacillus bulgaricus 107CFU/mL, streptococcus thermophilus 107CFU/mL and lactobacillus plantarum ST-
III 107CFU/mL is seeded in the cow's milk after step (1) processing to ferment under the conditions of 42 DEG C, after fermentation acidity reaches 77 ° of T,
Terminate fermentation.Turn over cylinder and be cooled to 22 DEG C, sterile filling is up to probiotics fermention of the present invention breast.
Comparative example 1
Commercially available fermented dairy product rich in lactobacillus plantarum ST-III.
Comparative example 2
The preparation method of this comparative example is same as embodiment 2, the difference is that only no added series bacillus CGMCC
No.8333 fermented supernatant fluids.
Comparative example 3
The preparation method of this comparative example is same as embodiment 4, the difference is that only no added valine.
Then the probiotics fermention breast prepared to embodiment 2-9 and comparative example 1-3 carries out index determining and sense organ is commented
It is fixed, specifically it is shown in Table 1.
1 each sample index determining of table and results of sensory evaluation
| Sample | ST-III clump counts | Structural state | Smell and flavour |
| Embodiment 2 | 109 | It is fine and smooth, uniform | Milk fragrance, mouthfeel are good |
| Embodiment 3 | 109 | It is fine and smooth, uniform | Milk fragrance, mouthfeel are good |
| Embodiment 4 | 109 | It is fine and smooth, uniform | Milk fragrance, mouthfeel are good |
| Embodiment 5 | 109 | It is fine and smooth, uniform | Milk fragrance, mouthfeel are good |
| Embodiment 6 | 109 | It is fine and smooth, uniform | Mango taste, mouthfeel are good |
| Embodiment 7 | 109 | It is fine and smooth, uniform | Strawberry flavor, mouthfeel are good |
| Embodiment 8 | 3×109 | It is fine and smooth, uniform | Milk fragrance, mouthfeel are good |
| Embodiment 9 | 3×109 | It is fine and smooth, uniform | Milk fragrance, mouthfeel are good |
| Comparative example 1 | 109 | It is fine and smooth, uniform | Milk fragrance, mouthfeel are good |
| Comparative example 2 | 107 | It is fine and smooth, uniform | Milk fragrance, mouthfeel are good |
| Comparative example 3 | 107 | It is fine and smooth, uniform | Milk fragrance, mouthfeel are good |
By contrast, it is newborn compared with commercially available ST-III acidified milks that the present invention prepares probiotics fermention, flavor and tissue
Structure is similar, free from extraneous odour, and rich in probiotics ST-III, and desired equipment, the cost that undertakes are decreased significantly；With comparative example 2-
3 compare, lactobacillus plantarum ST-III, improve two orders of magnitude, significant effect.Often eat probiotics provided by the invention
Acidified milk, balances human body intestinal canal flora, and improving immunity of organisms, reducing blood lipid, norcholesterol etc. has great help.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Any modification, equivalent substitution and simple modifications for being made in content etc., should all be included in the protection scope of the present invention.
Claims (9)
1. a kind of probiotics fermention breast, it is characterised in that the acidified milk is made of the raw material of following mass percent：Leavening
0.001%-0.006%, lactobacillus plantarum ST-III 0.001%-0.006%, lactobacillus plantarum ST-III multiplication agents 16%-
33%, stabilizer 0.25%-0.5%, sweetener 1%-8%, surplus are that the sum of raw milk, each component mass percent is
100%.
2. probiotics fermention breast according to claim 1, it is characterised in that the lactobacillus plantarum ST-III multiplication agents are classes
Fermented supernatant fluid, leucine, isoleucine, valine, tyrosine, methionine or the phenylpropyl alcohol of bacillus CGMCC No.8333
The fermented supernatant fluid additive amount of one or more in propylhomoserin, wherein series bacillus CGMCC No.8333 is 15%-30%,
A variety of amino acid additive amounts are 1%-3%.
3. probiotics fermention breast according to claim 1, it is characterised in that the raw milk is fresh milk or processing breast.
4. probiotics fermention breast according to claim 1, it is characterised in that the lactobacillus plantarum ST-III additive amounts are
106-108CFU/mL。
5. probiotics fermention breast according to claim 1, it is characterised in that the leavening is lactobacillus bulgaricus, thermophilic
One or more in hot streptococcus, Lactobacillus casei, bifidobacterium lactis or Lactobacillus rhamnosus；The leavening additive amount
For 106-108CFU/mL。
6. probiotics fermention breast according to claim 1, it is characterised in that fruit juice, jam, perfume (or spice) are further included in the acidified milk
One or more in essence, chocolate, coffee or mouthfeel modifying agent, content 1%-10%.
7. probiotics fermention breast according to claim 1, it is characterised in that oligofructose, oligomeric is further included in the acidified milk
One or more in galactolipin, inulin or poly- glucose sugar, content 1%-1.5%.
8. a kind of method for preparing probiotics fermention breast, this method comprise the following steps：
(1) raw milk, lactobacillus plantarum ST-III multiplication agents, sweetener and stabilizer are mixed, stirred evenly, homogeneous, heat is killed
Bacterium is handled, cooling；
(2) leavening and lactobacillus plantarum ST-III are seeded in the mixture after step (1) processing and carry out fermentation process, acid
Angle value reaches 70-85oFermentation is terminated during T；Cylinder cooling is turned over, cylinder cooling temperature is turned over as 15-25 DEG C, up to the probiotics fermention
Breast.
9. the method according to claim 8 for preparing probiotics fermention breast, it is characterised in that raw milk in step (1),
Temperature when multiplication agent, sweetener and stabilizer mix is 45-55 DEG C, mixing time 5-15min；The homogenizing temperature is
55-65 DEG C, level-one homogenization pressure is 15-20MPa, and double-stage homogenization pressure is 2-5MPa；The sterilization conditions are temperature 85-95
DEG C, time 10-25min；The cooling temperature is 38-45 DEG C.
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| CN109430397A (en) * | 2018-12-25 | 2019-03-08 | 光明乳业股份有限公司 | A kind of functional form acidified milk and preparation method thereof |
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| CN111500520B (en) * | 2020-06-02 | 2023-05-02 | 光明乳业股份有限公司 | Lactobacillus plantarum culture method |
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| CN115886074A (en) * | 2022-12-12 | 2023-04-04 | 甘肃普诺贝康生物科技有限责任公司 | Fermented milk and preparation method thereof |
| CN116616344B (en) * | 2023-07-26 | 2023-10-03 | 山东蒙天乳业有限公司 | Low-sugar lactobacillus beverage and preparation method thereof |
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