CN106577593A - High purity and high survival rate live ant acquisition method - Google Patents

High purity and high survival rate live ant acquisition method Download PDF

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Publication number
CN106577593A
CN106577593A CN201610988721.7A CN201610988721A CN106577593A CN 106577593 A CN106577593 A CN 106577593A CN 201610988721 A CN201610988721 A CN 201610988721A CN 106577593 A CN106577593 A CN 106577593A
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China
Prior art keywords
ant
container
living
acquisition method
nest
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CN201610988721.7A
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Chinese (zh)
Inventor
林芳花
陈志光
陈洁萍
陈鹤廷
陈捷阳
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Huizhou University
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Huizhou University
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Priority to CN201610988721.7A priority Critical patent/CN106577593A/en
Publication of CN106577593A publication Critical patent/CN106577593A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01MCATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
    • A01M1/00Stationary means for catching or killing insects
    • A01M1/20Poisoning, narcotising, or burning insects
    • A01M1/2022Poisoning or narcotising insects by vaporising an insecticide
    • A01M1/2027Poisoning or narcotising insects by vaporising an insecticide without heating
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01MCATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
    • A01M1/00Stationary means for catching or killing insects
    • A01M1/10Catching insects by using Traps
    • A01M1/103Catching insects by using Traps for crawling insects

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  • Life Sciences & Earth Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Engineering & Computer Science (AREA)
  • Insects & Arthropods (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Toxicology (AREA)
  • Catching Or Destruction (AREA)

Abstract

The invention belongs to the field of biology, and especially relates to a biological acquisition method. The method comprises: firstly, using the habit that live ants fear water, slowing filling water into a container which holds an ant nest, to separate the live ants from the ant nest, to obtain high purity live ants, and then using the characteristic that diethyl ether and other volatile general anesthesia liquid drugs can dizzy creatures by low dosage, and putting the live ant individuals into the clean container under the condition of swoon. The method can obtain high purity and high survival rate live ants, and is suitable for acquisition of red imported fire ants, white ants, and polyrhachis dives, and other medical ants.

Description

A kind of ant acquisition method living of high cleanliness and high-survival rate
Technical field
The invention belongs to field of biology, more particularly to a kind of biological acquisition method.
Background technology
Red fire ant and Coptotermes formosanus Shtrari. are to endanger two kinds of serious biologies to the mankind.Both biologies all live in the ant nest of underground In, there are hundreds thousand of to millions of individualities in each ant nest, belong to social insect.
When experiment interior carries out killing the ant test of pesticide effectiveness to red fire ant or Coptotermes formosanus Shtrari., experimenter is typically by red fire ant living Or Coptotermes formosanus Shtrari. brush pen is gently provoked one by one from ant nest, in being put into the vial of wide-mouth, impose medicament and observed.But due to Red fire ant or Coptotermes formosanus Shtrari. living can arbitrarily creep, and chosen wastes time and energy to wide mouthed bottle, and polypide is soft, and slightly imprudence is just held Easily polypide is killed, or polypide is still living when choosing in wide mouthed bottle, but brush pen is stabbed, soon will be dead, The accuracy of the test of pesticide effectiveness is affected, is often chosen into wide together with grogs when directly choosing from ant nest red fire ant or Coptotermes formosanus Shtrari. in addition Mouth bottle, polypide degree of purity is low.And, red fire ant also has the habit of the active attack mankind, and experimenter is often red when operating Fiery ant bites.Therefore there is very big defect in the experimental technique.
Polyhachis vicina Roger(Polyrhachis vicina Roger)And bitooth multi-ant(Polyrhachis dives Smith)It is two kinds of main medical ants.
Polyhachis vicina Roger mainly live in nature have abundant water resources, the weedtree of the forest that the density of trees is high and river bank It is social insect that population lives on trees in woods, pine forest, bamboo grove, camellia oleifera lam, fruit tree woods, often builds nest in tree is upper or underground, seeks colony Social life.Polyhachis vicina Roger(Black ant)With the kidney invigorating, strong bone, defying age, disease preventing and treating medical value, can as medicine or The raw material of health product.Yunnan Province's medical material standard version in 1996 is recorded:This product is the dry body of ant insect Polyhachis vicina Roger.The four seasons Can adopt, even ant nest is included suffocated in closed bag in the lump, or scald dead in input boiling water;Take out, remove ant nest, leaveves and soil etc. miscellaneous Matter, ant body is put and dry under daylight or less than 60 DEG C dry.
Bitooth multi-ant is a kind of insecticide of Formicidae Polyrhachis.Nest in the tree more than bitooth multi-ant and occupy, minority is built Nest is under thick grass, stone.In the winter time ant nest can be by being transferred to ground on tree.Ant nest is by tree branches, weeds chip, spinning thing etc. Constitute, in cellular in nest, there are many cells.Bitooth multi-ant have antiinflammatory, antalgic and sedative, spasmolytic of relievining asthma, hepatoprotective it is medicinal Value, can be used as medicine or the raw material of health product.Hunan Province's Chinese crude drug standard(2009 editions)Record:This product is ant insect bidentate The adult dry body of thorniness ant.Caught before March November to next year, put in appropriate vessel and suffocate, take out drying, or it is fast with 60 DEG C of water Speed scalds dead, dries.
The acquisition method of above two medical ant is wasted time and energy, and the body surface of polypide can adhere to soil or microorganism, Degree of purity is low.
The content of the invention
In view of this, the technical problem to be solved is to provide a kind of high cleanliness and the ant living of high-survival rate is adopted Diversity method.
The technical problem to be solved is achieved by the following technical programs:
The ant acquisition method living of a kind of high cleanliness and high-survival rate is:
(1)Last layer Pulvis Talci is applied at the edge of container, is taken whole ant nest with spade and is put in container;
(2)The little wooden branch of multistage on the tip shelf of ant nest;
(3)Slowly add water toward container, water is added at ant nest top 1/10 ~ 2/10, the speed for adding water is slow, so as to the ant that lives Can up withdraw in time;
(4)After the ant that lives is gathered in the wooden branch on the top of ant nest, gently the wooden branch for gathering ant living are put in advance with long branch In ready another clean container, cover, and put protective bag;
(5)By container band to operating room, lid is opened rapidly, the volatility general anesthesia liquid of Deca anaesthesia dosage into container Medicine;
(6)Ant to be lived at once is shaken off ant living from wooden branch by daze, takes out wooden branch, is proceeded in another container with spoon, Sealing, it is standby.
Further, the ant living is the tiny reptile afraid of water of the social sexual life of the battalion lived on trees.
Further, the tiny reptile afraid of water of the battalion's mass society life lived on trees includes red fire ant Or Coptotermes formosanus Shtrari. or medical ant.
Further, the medical ant includes Polyhachis vicina Roger or bitooth multi-ant.
Further, step(5)The volatility general anesthesia liquid medicine include ether, enflurane, isoflurane, fluorine At least one in alkane, Sevoflurane.
Further, step(5)The volatility general anesthesia liquid medicine is ether, and 2L containers consumption is 2 ~ 4 drops.
The present invention has the advantages that:
1st, the present invention fears the habit of water first with red fire ant or Coptotermes formosanus Shtrari., using to the side of slow water filling in the container equipped with ant nest Method, ant living is separated from ant nest, the ant living of high cleanliness is obtained, thereafter with volatile general anesthesia medicine such as ether With low dosage can the biological habit of daze, the ant individuality that makes to live is loaded into test container or other appearances in the case of by daze In device, the process is compared the method that existing brush pen chosen in ant living to wide mouthed bottle and has been saved the plenty of time, and significantly improves Survival rate.
2nd, the ant living of the high cleanliness obtained by the present invention, for the Formica fusca such as Polyhachis vicina Roger or bitooth multi-ant as raw material Production medicine or health product, can reduce the soil that traditional method harvests Formica fusca institute band, improve the cleanliness factor of raw material, reduce with It is the content that the impurity of raw material production medicine or health product is particularly heavy metal, with entering to avoid caused by soil and dead ant Microorganism pollution, so as to improve the quality of product.And gained is lived ant survival rate 100%, and the volatility general anesthesia such as ether Liquid medicine is volatile, noresidue, it is ensured that human or animal eats or pharmaceutical safety.
Specific embodiment
With reference to embodiment, the present invention will be described in detail, and embodiment is only the preferred embodiment of the present invention, It is not limitation of the invention.
Embodiment 1
Red fire ant test of pesticide effectiveness, red fire ant individuality need to be put in wide mouthed bottle impose medicament.Test preparation is as follows:
(1)Last layer Pulvis Talci is applied at the edge of Plastic Drum, is taken whole ant nest with spade and is put in Plastic Drum;
(2)The little wooden branch of multistage on the tip shelf of ant nest;
(3)Slowly add water toward Plastic Drum, water is added at ant nest top 1/10, the speed for adding water is slow, so as to ant energy of living Up withdraw in time, wait about 1 hour;
(4)After the ant that lives is gathered in the wooden branch on the top of ant nest, gently the wooden branch for gathering ant living are put in advance with long branch In the milk pot of ready clean 2L, cover, and put protective bag;
(5)By milk pot band to laboratory, lid is opened rapidly, the drop of Deca 2 ether into container;
(6)Ant to be lived at once is shaken off it from wooden branch by daze, takes out wooden branch, and with spoon corresponding wide mouthed bottle is proceeded to In, sealing is standby.Wait a moment, the ant living proceeded in wide mouthed bottle can all recover, survival rate 100%.
The method fears the habit of water first with red fire ant, using the method to slow water filling in the container equipped with ant nest, Ant living is separated from ant nest, the red fire ant for obtaining high cleanliness is individual, have the low dosage can daze life with ether thereafter The habit of thing, the ant individuality that makes to live is loaded in test wide mouthed bottle in the case of by daze, and the process is compared and existing uses brush pen The method chosen in ant living to wide mouthed bottle has been saved the plenty of time, and significantly improves survival rate.
Embodiment 2
Wild Polyhachis vicina Roger is gathered, method is as follows:
(1)Last layer Pulvis Talci is applied at the edge of Plastic Drum, is taken whole ant nest with spade and is put in container;
(2)The little wooden branch of multistage on the tip shelf of ant nest;
(3)Slowly add water toward Plastic Drum, water is added at ant nest top 1/10 ~ 2/10, the speed for adding water is slow, to live Ant can up be withdrawn in time;
(4)After the ant that lives is gathered in the wooden branch on the top of ant nest, gently the wooden branch for gathering ant living are put in advance with long branch In ready clean 2L milk pots, cover, and put protective bag;
(5)By milk pot band to operating room, lid is opened rapidly, the drop of Deca 3 ether into container;
(6)Treat that Polyhachis vicina Roger, by daze, is at once shaken off it from wooden branch, take out wooden branch, with spoon another appearance is proceeded to In device, sealing is standby.
Wait a moment, the recovery of wild Polyhachis vicina Roger, survival rate 100%, then carry out with wild Polyhachis vicina Roger as medicine or The making of health product.
The method is equally applicable to bitooth multi-ant.
The method collecting efficiency is high, high purity, and survival rate is high, can reduce traditional method harvest wild Polyhachis vicina Roger or The soil of bitooth multi-ant institute band, improves the cleanliness factor of raw material, reduces with impurity that it is raw material production medicine or health product The particularly content of heavy metal, with the microorganism pollution for entering to avoid soil and caused by dead ant, so as to improve the quality of product. And gained is lived ant survival rate 100%, and the volatility general anesthesia liquid medicine such as ether is volatile, noresidue, it is ensured that people or Animal edible or pharmaceutical safety.

Claims (6)

1. the ant acquisition method living of a kind of high cleanliness and high-survival rate, it is characterised in that:
(1)Last layer Pulvis Talci is applied at the edge of container, is taken whole ant nest with spade and is put in container;
(2)The little wooden branch of multistage on the tip shelf of ant nest;
(3)Slowly add water toward container, water is added at ant nest top 1/10 ~ 2/10, the speed for adding water is slow, so as to the ant that lives Can up withdraw in time;
(4)After the ant that lives is gathered in the wooden branch on the top of ant nest, gently the wooden branch for gathering ant living are put in advance with long branch In ready another clean container, cover, and put protective bag;
(5)By container band to operating room, lid is opened rapidly, the volatility general anesthesia liquid of Deca anaesthesia dosage into container Medicine;
(6)Ant to be lived at once is shaken off ant living from wooden branch by daze, takes out wooden branch, is proceeded in another container with spoon, Sealing, it is standby.
2. according to the ant acquisition method living described in claim 1, it is characterised in that:The ant living is the social sexual life of the battalion lived on trees Tiny reptile afraid of water.
3. ant acquisition method living according to claim 2, it is characterised in that:It is thin that the battalion's mass society lived on trees is lived Little reptile afraid of water includes red fire ant or Coptotermes formosanus Shtrari. or medical ant.
4. ant acquisition method living according to claim 3, it is characterised in that:The medical ant include Polyhachis vicina Roger or Bitooth multi-ant.
5. ant acquisition method living according to claim 1, it is characterised in that:Step(5)The volatility general anesthesia liquid Body medicine includes at least one in ether, enflurane, isoflurane, desflurane, Sevoflurane.
6. ant acquisition method living according to claim 1, it is characterised in that:Step(5)The volatility general anesthesia liquid Body medicine is ether, and 2L containers consumption is 2 ~ 4 drops.
CN201610988721.7A 2016-11-10 2016-11-10 High purity and high survival rate live ant acquisition method Pending CN106577593A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112674261A (en) * 2021-01-04 2021-04-20 贵州金山蚂蚁保健品有限公司 Method for reducing heavy metal content of polyrhachis vicina roger

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110214340A1 (en) * 2010-03-04 2011-09-08 Arnold Gregory Klein Ant barrier for hummingbird feeders
CN102217580A (en) * 2010-04-16 2011-10-19 蚂蚁陷阱有限公司 Improved ant trap
CN202122017U (en) * 2011-06-13 2012-01-25 深圳职业技术学院 Collection device for winged red imported fire ants
CN103053491A (en) * 2013-01-30 2013-04-24 广东省昆虫研究所 Method for collecting young red imported fire ants
CN204860700U (en) * 2015-08-05 2015-12-16 中国农业科学院草原研究所 Open -air insect collection device

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110214340A1 (en) * 2010-03-04 2011-09-08 Arnold Gregory Klein Ant barrier for hummingbird feeders
CN102217580A (en) * 2010-04-16 2011-10-19 蚂蚁陷阱有限公司 Improved ant trap
CN202122017U (en) * 2011-06-13 2012-01-25 深圳职业技术学院 Collection device for winged red imported fire ants
CN103053491A (en) * 2013-01-30 2013-04-24 广东省昆虫研究所 Method for collecting young red imported fire ants
CN204860700U (en) * 2015-08-05 2015-12-16 中国农业科学院草原研究所 Open -air insect collection device

Non-Patent Citations (2)

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Title
丛福君 等: "石狩红蚁人工采集方法初步研究", 《森林病虫通讯》 *
吕利华 等: "介绍红火蚁的野外采集和实验室饲养的方法", 《昆虫知识》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112674261A (en) * 2021-01-04 2021-04-20 贵州金山蚂蚁保健品有限公司 Method for reducing heavy metal content of polyrhachis vicina roger

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Application publication date: 20170426

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