CN106576893A - Three-dimensional box for efficient and environment-friendly culture of lactarius deliciosus and in-box culture method for lactarius deliciosus - Google Patents

Three-dimensional box for efficient and environment-friendly culture of lactarius deliciosus and in-box culture method for lactarius deliciosus Download PDF

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Publication number
CN106576893A
CN106576893A CN201611011365.XA CN201611011365A CN106576893A CN 106576893 A CN106576893 A CN 106576893A CN 201611011365 A CN201611011365 A CN 201611011365A CN 106576893 A CN106576893 A CN 106576893A
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China
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bacterium
red fir
fir bacterium
cultivation
nutritive layer
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CN201611011365.XA
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余虹仪
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Individual
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/20Culture media, e.g. compost
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/60Cultivation rooms; Equipment therefor

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  • Life Sciences & Earth Sciences (AREA)
  • Mycology (AREA)
  • Environmental Sciences (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention relates to a three-dimensional box for efficient and environment-friendly culture of lactarius deliciosus and belongs to the field of original-ecological three-dimensional culture of the lactarius deliciosus. Hierarchical sheets are transversely and uniformly distributed in a culture box, culture shelves are uniformly placed at the internal bottom of the culture box and on the hierarchical sheets, trays are fixed at lower ends of lining tubes, worm wheels close to the lower ends are matched with worms, and nutritive culture layers are laid on the lining tubes in an enclosing manner. Connecting pipes at end parts of the culture shelves are connected with five-way tubes which are connected with a clean water tank pipeline, a disinfecting solution tank pipeline, a nutrient solution storage tank pipeline and an air accumulator pipeline. The three-dimensional box for the efficient and environment-friendly culture of the lactarius deliciosus is simple and reasonable in structure, the culture of the lactarius deliciosus is convenient and easy in operation, and pesticides are not used during culture, so that large-scale and industrialized culture of the lactarius deliciosus is facilitated; wild-environment-imitated original-ecological lactarius deliciosus culture is adopted, so that the yield is high, the quality is good, and the commercial value of industrialization is high; and a lactarius deliciosus culture medium is matched with a nutrient solution and can be applied to repeated culture repeatedly, so that the culture cost of the lactarius deliciosus is reduced.

Description

Efficient green cultivates breeding method in the case of red fir bacterium solid case and red fir bacterium
Technical field
The present invention relates to a kind of efficient green in the red fir bacterium field of stereo cultivation of simulated primitive ecology cultivates red fir in three-dimensional case Breeding method in the case of bacterium solid case and red fir bacterium.
Background technology
Red fir bacterium is the hemispherical mushroom of medial recess, is the wild wild mushroom for being grown in pine forest, because of delicious, nutritive value It is high and standby welcomed by the people;But the difficulty of artificial culture is very big, cause to there is no that red fir bacterium is sold on market.In order that Red fir bacterium can become the daily delicacies on people's dining table, and we carry out test of many times at the artificial stereoscopic culture to red fir bacterium, Repeatedly summing up experience and lesson after failure, cultivates through the research up to 7 years as long as and test, have successfully completed finally The new industrial research of the red fir bacterium of artificial stereoscopic culture.Meanwhile, we also devise suitable for family cultivate on a small scale red fir bacterium and The stereo cultivation case of red fir bacterium is cultivated in industrialization.
The content of the invention
The purpose that the present invention is related to is to provide for a kind of green organic of the efficient, high-quality in stereo cultivation case and cultivates The efficient green of red fir bacterium cultivates breeding method in the case of red fir bacterium solid case and red fir bacterium.
For achieving the above object, efficient green of the present invention cultivates red fir bacterium solid case mainly includes incubator, cold Freeze heat sink or cold air heat sink, heater, level plate, culturing frame, cultivation nutritive layer, clean water tank, disinfectant solution tank, battalion Foster fluid reservoir, air accumulator;Described culturing frame includes fixed culturing frame and rotatable culturing frame.
Heater and frozen cooling device or cold air heat sink are installed in incubator, one before incubator Face is provided with door, the both sides of incubator and it is disposed behind have vent window, the laterally uniform plate that has levels in incubator, Level plate is fixed on the both sides in incubator, or, level plate is arranged on and is fixed on the fixed horizontal stripe of the both sides in incubator; Bottom in incubator constitutes multi-level cultivation layer with level plate, and bottom and top and the level plate of incubator are parallel to each other. Fixed culturing frame or rotatable culturing frame are uniformly placed with above bottom and every plate secondary from level to level in incubator, are being fixed Cultivation nutritive layer is fixed with the pallet of culturing frame or on the pallet of rotatable culturing frame;Each fixes culturing frame or rotatable Culturing frame is one and cultivates row have multiple cultivation to go on the bottom and each level plate in incubator;It is fixed on fixed cultivation The connecting tube of the end of one end of frame or rotatable culturing frame is connected with Five-way tube port, and remaining four of Five-way tube are led to Mouth connecting pipe respectively with nutrition fluid reservoir, the connecting pipe of air accumulator, the connecting pipe of disinfectant solution tank and clean water tank Connecting pipe connection, in connection clean water tank, disinfectant solution tank, air accumulator, be separately installed with the pipeline of nutrition fluid reservoir The switch of control.Top below per plate secondary from level to level in one side and incubator is provided with sprayer unit, electric filament lamp, green glow Lamp and high intensity flasher, the circuit of electric filament lamp, green light lamp and high intensity flasher is connected with light-illuminating controller, lamp When the circulation opening time of the light of light irradiation controller control electric filament lamp, green light lamp and high intensity flasher and circulation are closed Between.
Described fixed culturing frame includes pedestal, stull, internal lining pipe, pallet, rubber blanket, connecting tube;Described rubber blanket It is that centre has the Gask-O-Seal in hole;Pallet is fixed with the internal lining pipe below via outside convergence hole, under internal lining pipe The convergence hole at end is socketed on the protrusion joint of impact faces, rubber blanket pad the inner of convergence hole with protrusion joint upper end it Between be tightly connected place, the end insertion of connecting tube or screw in the connecting hole of base ends, the upper through hole and internal lining pipe in pedestal The internal run-through of insertion, clear opening and connecting tube;Stull is fixed on the both sides of pedestal and stably fixes culturing frame not to both sides inclination, Connecting tube is fixed on the rear and front end of pedestal and pedestal is together in series and culturing frame not rear-inclined forward is stably fixed, fixed to cultivate The connecting hole of last pedestal of frame one end has plug to block passage, last pedestal for the culturing frame other end that is connected Connecting tube be connected with one end of Five-way tube;One fixed culturing frame has multiple pedestals, multiple stulls, multiple internal lining pipes and multiple Pallet.
Described rotatable culturing frame includes pedestal, stull, internal lining pipe, pallet, turbine, worm screw, the gasket seal that trackslips, company Adapter;The described gasket seal that trackslips creates the compound gasket that upper and lower surface can mutually trackslip and seal, compound gasket Centre have through hole;In the end insertion of connecting tube or the connecting hole of screw-in base ends, the upper through hole and internal lining pipe in pedestal The internal run-through of insertion, clear opening and connecting tube;The convergence hole of the lower end of internal lining pipe is socketed on the protrusion joint of impact faces, The gasket seal pad that trackslips is tightly connected place between inner and protrusion joint the upper end of convergence hole;Lower end outside internal lining pipe or Turbine is fixed with lower end, pallet is fixed with the internal lining pipe between nethermost via above turbine;Turbine Coordinate with worm screw, worm screw can drive turbine to rotate, the axle of worm screw directly with the axle Hard link of adjustable frequency motor or be flexible coupling, or Person, the axle of worm screw is connected again by variator with the axle of common motor;Stull is fixed on the both sides of pedestal and stablizes rotatable training Educate frame not incline to both sides, connecting tube is fixed on the rear and front end of pedestal and is together in series pedestal and stablizes culturing frame not forwards, backwards Incline, the connecting hole of last pedestal of rotatable culturing frame one end has plug to block passage, connects rotatable culturing frame another The connecting tube of last pedestal of one end is connected with one end of Five-way tube;One rotatable culturing frame has multiple pedestals, multiple Stull, multiple internal lining pipes and multiple pallets;Described turbine creates an entirety or is sleeved under internal lining pipe with internal lining pipe The outside of the convergence hole in portion.
Manufacture above described pedestal has less than the lower sections of protrusion joint of pedestal, and the right and left of pedestal can be consolidated Determine stull, the front-end and back-end of pedestal are manufactured with manufacture in connecting hole, pedestal has clear opening with the front-end and back-end of pedestal Connecting hole insertion, the upper through hole of and insertion vertical with the clear opening in pedestal wears out protrusion joint lower sections of less than pedestal Upper surface, clear opening forms interior threeway with upper through hole.
Described stull creates hollow or solid cross-brace frame;After described pedestal is separately manufactured with stull Assembly or create an entirety;Described internal lining pipe creates hollow pipe, and the lower end manufacture of internal lining pipe has linking Manufacture around hole, the upper end of internal lining pipe and the internal lining pipe of convergence hole above section has via, in convergence hole upper part The hollow parts of bushing pipe create nutrition supply chamber;Described pallet creates centre the flat board or disk in hole, pallet set Be mounted on internal lining pipe or pallet and internal lining pipe create an entirety, pallet is to support the carrier for cultivating nutritive layer, pallet with Internal lining pipe coordinates can fix cultivation nutritive layer;Described connecting tube creates hollow siphunculus, for conveying nutritional solution, disappearing Venom, clear water and compressed air;Described via creates the hole for allowing nutritional solution, disinfectant solution, clear water and compressed air to pass through, Via is used to convey nutritional solution, disinfectant solution, clear water and compressed air to cultivation nutritive layer;Described nutrition supply chamber is used to transmit Nutritional solution, disinfectant solution, clear water and compressed air are at via.
Described cultivation nutritive layer is that red fir bacterium cultivates base, is the part for storing nutritional labeling and the combination for cultivating red fir bacterium The solid layer of composition, be cultivate red fir bacterium nutritional labeling materials processing become remix after powder together add Folium Pini water after Fermentation body;Described Folium Pini water is the water juice being boiled into Folium Pini;The material of described red fir bacterium culture medium includes:Ramulus Pini, jade Meter Xin, pine forest thin sand and soil, rice husk, Gypsum Fibrosum, white sugar, the ratio of its composition is:5 parts of Ramulus Pini, 2 parts of corn cob, pine forest thin sand and soil 2 Part, 1 part of rice husk, 1 part of Gypsum Fibrosum, 1 part of white sugar.Described cultivation nutritive layer be able to can repeat to cultivate red fir with nutritious liquid prescription Bacterium, and ensure that the quality of the red fir bacterium cultivated.
Described clean water tank is the tank body for containing clear water;Described disinfectant solution tank is the tank body for containing disinfectant solution, described Disinfectant solution is lime water;Described nutrition fluid reservoir is the tank body for containing nutritional solution;Described air accumulator is storage pressure The tank body of contracting air, the air in tank has pressure;Described nutrition fluid reservoir, air accumulator, disinfectant solution tank and clean water tank are The separate component being individually separated, or, air accumulator is combined into a multi-cavity with nutrition fluid reservoir, disinfectant solution tank and clean water tank United joint tank;Air accumulator is combined into a multi-cavity and joins in combination with nutrition fluid reservoir, disinfectant solution tank and clean water tank Close tank when, combine tank inside by divides become nutrition sap cavity, sterilization sap cavity and clear water chamber three it is each self-packing Chamber, in passage connection nutrition sap cavity, three chambers of sterilization sap cavity and clear water chamber on demarcation strip top, nutrition sap cavity, sterilization sap cavity With the part for above three chambers of clear water chamber being partly storing compressed air, nutrition sap cavity, three chambers of sterilization sap cavity and clear water chamber Below be partly the part for storing nutritional solution, disinfectant solution and clear water, what is be connected with nutrition supply chamber conveys the pipeline of nutritional solution Nutritional solution mouth, conveying disinfectant solution pipeline disinfectant solution mouth and conveying clear water pipeline clean water outlet all joint tank inside The bottom of cavity, the gas tube orifice of the compressed air piping being connected with nutrition supply chamber is at the top of the internal cavities for combining tank;Connection The compressed air closed in tank serves the effect of pump above nutritional solution, disinfectant solution and clear water and reduces infusion pump.
In order that turn out the red fir bacterium for coming yield is higher, quality more preferably, will be to training in the incubation of red fir bacterium Educate conveying nutritional solution in nutritive layer;Described nutritional solution be by the slurry that breaks into after Testa Tritici, Folium Pini, Semen Allii Tuberosi, Semen Pini, Gypsum Fibrosum mixing again The emulsion of Jing everfermentations after a small amount of white sugar and potassium dihydrogen phosphate mix homogeneously is added, then the emulsion elimination slag after fermentation Obtain without slag emulsion, the ratio of nutritional solution material composition is:15 parts of Testa Tritici, 6 parts of Folium Pini, 3 parts of Semen Allii Tuberosi, 2 parts of Semen Pini, white sugar 1 Part, 1 part of Gypsum Fibrosum, 1 part of potassium dihydrogen phosphate.Biphosphate potassium composition is added in nutritional solution, make the nutrition of red fir bacterium culture medium into Divide and keep permanent, and the fast-growth of red fir bacterium can be promoted, make the fresh and tender of red fir bacterium that grow out;Pine is added in nutritional solution Pin composition, the nutritional labeling for making red fir bacterium culture medium keeps permanent, and can promote the fast-growth of red fir bacterium, makes to grow out Red fir bacterium it is bright-colored and fresh and tender;Gypsum component is added in nutritional solution, the clean taste of red fir bacterium can be made fine and smooth;In battalion Testa Tritici composition is added in nutrient solution, the fast-growth of red fir bacterium and the fair and tender degree of the red fir bacterium of increase can be promoted;Add in nutritional solution Enter Semen Allii Tuberosi composition, the fast-growth of red fir bacterium can be promoted, increase the nutritional labeling and exquisiteness of red fir bacterium, make the mouth of red fir bacterium Feel soft and smooth exquisiteness.
In order to prevent convey in nutritive layer after nutritional solution to cultivating, the moisture cultivated in nutritive layer is too high, employs Incline dewatering and blowing is dried two methods to reduce the moisture cultivated in nutritive layer;Described inclination dewatering be by incubator and Level plate inclines certain angle;It is to blow to training through via from nutrition supply chamber by compressed air that described blowing is dried In educating nutritive layer, air is taken the unnecessary moisture in cultivation nutritive layer outside cultivation nutritive layer out of.
In order to increase cultivation face, placed using two inclined-planes and cultivate nutritive layer to increase cultivation face.
In order that red fir bacterium has wild taste, fog is sprayed using sprayer unit, make red fir bacterium obtain rain and dew;Using white Vehement lamp makes red fir bacterium obtain wild environment with the flash irradiation of the moment of the circulation irradiation and high intensity flasher of green light lamp Growth conditionss, the ability that the ring grain ring on the cap of red fir bacterium can be promoted to form and strengthen the absorption nutritional labeling of red fir bacterium.
In order that the red fir bacterium vaccine cultivated on nutritive layer can uniformly receive the irradiation of light, in irradiation light light, The power supply for driving worm screw is first connected, makes worm screw drive turbine to rotate, then open the switch of light, the light for making light is radiated at On the vaccine cultivated on nutritive layer of rotation.
The material of the inoculation bacterium bag of red fir bacterium is mixed using addition white sugar after the crushing of pine sawdust, Testa oryzae or Testa Tritici and Gypsum Fibrosum powder It is even, add Folium Pini water mix it is wet after form, the ratio of its composition is pine sawdust 78%, Testa oryzae or Testa Tritici 20%, white sugar 1%, stone Cream powder 1%.In order that the bacterium bag of postvaccinal red fir bacterium is easy to be trapped among on the face of the cultivation nutritive layer outside internal lining pipe, it is described Inoculation bacterium bag makes the bacterium bag of rectangular thin type.Enclose the cultivation nutrition being taped against on internal lining pipe after postvaccinal bacterium bag is cut off again On the surface of layer, enclose be taped against it is fixed on the surface for cultivating nutritive layer after blowed to through via from nutrition supply chamber with compressed air Cultivate nutritive layer in, the mycelia for making inoculation rocks with compressed air, reach wake up mycelia emerge and long bacterium purpose;Reduce biography System cultivate manually scratching during red fir bacterium wrap wake up mycelia emerge and long bacterium program, while decreasing manually scratch bag When the waste of the material of red fir bacterium culture medium drawn out.
In order that red fir bacterium culture medium fixation and allows inoculum bag in red fir bacterium culture medium fix tightly on internal lining pipe Gu, red fir bacterium culture medium is held using pallet and is fixed on internal lining pipe with interior fixed net, using outer fixed net by inoculum bag It is fixed on red fir bacterium culture medium.
Nurturing an environment in described incubator is to imitate the nurturing an environment under wild natural ecological environment, using in training Educate the measure of the interior setting insulation of case and cooling to realize temperature control;Without following the Seasonal Regularity of cultivation when cultivating red fir bacterium, Can start the imitative wild nurturing an environment in incubator at any time in arbitrary season and arbitrary natural environment and be had Machine biological breeding;Collect imitative Wild ecological environment with organic cultivation and stereo cultivation in each incubator, realize organic vertical The automated management that body agricultural is cultivated.
Described frozen cooling device and cold air heat sink is heat sink, using freezing cooling water circulation, or, adopt Lowered the temperature with circulating cold air, or, lowered the temperature using compressor cooling;Described heater be using electric energy heating heating tube or Steam circulation heating tube or hot-air blower, are heat risers.
In order to improve the quality of red fir bacterium and the growth beneficial to red fir bacterium, pallet and internal lining pipe are during fabrication using nontoxic Plastics or glass steel or stainless steel are manufactured.
In order that nutritional solution can be input in cultivation nutritive layer, nutrition fluid reservoir, disinfectant solution tank and clean water tank and sky The internal cavity of gas accumulator creates the joint cavity of an entirety, and compressed air is input into joint cavity using inflating pump It is interior, the pressure instead work of infusion pump that compressed air is produced;Or, by nutrition fluid reservoir, clean water tank and disinfectant solution tank peace It is mounted in above incubator;Or, in the tank of nutrition fluid reservoir, clean water tank and disinfectant solution tank or connection nutrition fluid reservoir, Infusion pump is installed in the pipeline of clean water tank and disinfectant solution tank.
In order that have air pressure in air accumulator or in joint tank, using inflating pump gases at high pressure input air accumulator In or joint tank in.
In order to prevent virus and can sterilize to cultivating nutritive layer, using 2-3% lime water to cultivating nutrition Layer carries out disinfection sterilization;The method of its disinfection is that 2-3% lime water is put in disinfectant solution tank, by pipeline and battalion Foster feeding chamber is input on red fir bacterium cultivation base to be planted and red fir bacterium cultivation base is carried out disinfection sterilization.
When red fir bacterium is cultivated in incubator, first the inoculation bacterium bag of red fir bacterium is sent into into freezing indoor subzero less than 15 DEG C Cold air freeze 10 hours, reach freezing sterilizing purpose;After sterilizing, the inoculation bacterium bag after defrosting is sent into 23 DEG C to 25 DEG C aseptic inoculating room in, by vaccine put into inoculation bacterium bag be inoculated with, complete within 24 hours inoculation;Afterwards, by postvaccinal bacterium Bag is sent into 25 DEG C to 32 DEG C of interior and is cultivated, and after 40 to 45 days, the mycelia that being inoculated with has white in bacterium bag is paved with bacterium bag and is Mycelia culture success;Afterwards, the inoculation bacterium bag for covering with mycelia is put in cultivation box and red fir bacterium is cultivated.
Before the inoculation bacterium bag for covering with mycelia is put in cultivation box, first with 2-3% lime water to cultivating nutritive layer Carry out disinfection sterilization;The method of its disinfection is that 2-3% lime water is put in disinfectant solution tank, by pipeline and nutrition Feeding chamber is input on red fir bacterium cultivation base to be planted and red fir bacterium cultivation base is carried out disinfection sterilization, and the input quantity of lime water is It is standard just to soak cultivation nutritive layer, cultivates the switch that sterilizing path is closed after nutritive layer soaks;After sterilization one day, pressure is opened The switch compressed air of contracting air dries up red fir bacterium and cultivates the switch that compressed air is closed after base;Afterwards, by incubator Temperature is adjusted to 25 DEG C to 32 DEG C, and more than 25 DEG C, in cultivation box are adjusted to heating devices heat when temperature is less than 25 DEG C Less than 32 DEG C are adjusted to when temperature is higher than 32 DEG C with frozen cooling device or the cooling of cold air heat sink;Afterwards, mycelia will be covered with Inoculation bacterium bag cut off after enclose fixed on the surface of the cultivation nutritive layer being taped against around internal lining pipe again;Covering with the inoculation bacterium of mycelia Encirclement be taped against cultivate nutritive layer surface on after mycelia is carried out scratching within first day disturb, be commonly called as scratching bag.
Scratch when disturbing mycelia, open the switch that airport is connected with nutrition supply chamber, compressed air is into nutrition supply intracavity By via enter cultivate nutritive layer after blow mycelia again, by compressed air rock mycelia three times, rock every time mycelia 10 to The air inlet switch of compressed air is closed after 20 minutes, mycelia can be waken up when rocking mycelia, make mycelia emerge and long bacterium, after one day Stopping is scratched and disturbs mycelia;Mycelia is grown after vaccine, and the root of vaccine is deeply cultivated in nutritive layer and absorbs nutrition and grow up, and now, to give Cultivate nutritive layer supply nutrition and carry out light-illuminating;The mode of supply nutrition adopts nutritional solution replenishment, goes out the initial stage of vaccine Per 2 days, supplement was once, supplement once daily into after bacterium;During extra-nutrition, the connection of nutrition fluid reservoir and nutrition supply chamber is opened Switch, nutritional solution is entered to be entered through via after nutrition supply intracavity and cultivated in nutritive layer, and the surface of nutritive layer to be cultivated occurs Moistening and closing switch during color burn, stop conveying nutritional solution;When the irradiation light light of fixed culturing frame is placed with incubator, Directly open the switch of light;When the irradiation light light of rotatable culturing frame is placed with incubator, drive worm screw rotation is first connected The power supply of the motor for turning, makes worm screw drive after turbine rotation and opens the switch of light again, and makes the light of light be radiated at rotation On the cultivation nutritive layer for turning, the efficient uniform for then obtaining the vaccine on cultivation nutritive layer, cut-out when closing light drives snail The power supply of the motor of bar rotation;During irradiation light light, the switch of lamp dimmer is opened, used again after being irradiated 1 minute with electric filament lamp Green glow light irradiation 5 minutes, electric filament lamp is closed after replacing irradiation with green light lamp 2 hours, and interval is after 1 hour again with electric filament lamp and green glow Lamp alternately irradiates, and opened high intensity flasher high intensity flash irradiation when green light lamp is opened every 9 hours and cultivates nutrition Layer 3 seconds, so circulation ceaselessly illumination until Mining harvests red fir bacterium till, the light for making light is radiated at the cultivation of rotation and seeks The light for obtaining vaccine on foster layer;The circulation irradiation of different light makes red fir bacterium obtain the growth conditionss of wild environment, energy The ability for enough promoting ring grain ring on the cap of red fir bacterium to form and strengthen the absorptions nutritional labeling of red fir bacterium, make cap hypertrophy, Bacterium bar is sturdy.
In the cultivating process of red fir bacterium, at least daily with the compressed air in air accumulator by nutrition supply chamber One is blown to secondary, at least 1 minute every time, make the root of red fir bacterium exist to increase the ventilation of organic soil to nutritive layer is cultivated Respiratory ventilation in organic soil, meanwhile, Jing often to open vent window to ventilation in incubator, it is ensured that the air in incubator It is fresh, and slightly spray a spray water with sprayer unit daily and make red fir bacterium obtain the dew of wild environment;Cultivating The red fir bacterium cultivated in case is ripe successively, and when the bacteria cover diameter of red fir bacterium reaches 8 to 10cm, the color of cap is purplish red and fresh Gorgeous, ring grain substantially, bacterium bar is mellow and full and appear yellowish pink, whole red fir bacterium is suffused an exquisite fragrance all around, and is now the ripe mark of red fir bacterium, and Mining is received Ripe red fir bacterium;The strain implantation of red fir bacterium to be cultivated and can again bear strain after nutritive layer, adds electric filament lamp and green light lamp Illumination and the lasting supplementary of nutritional solution cultivate nutritive layer, daily Mining is received to enable red fir bacterium, is continued Mining and is received for up to 18 Month, complete the overall process of the green organic stereo cultivation of a round of the red fir bacterium of high-quality in incubator.
Efficient green of the present invention cultivates the simple for structure, reasonable of red fir bacterium solid case, is adapted to the small-scale red fir bacterium of family The red fir bacterium of cultivation with industrialization is cultivated, red fir bacterium is cultivated conveniently and is easily operated, be easy to scale and industrialization to cultivate red fir Bacterium, the yield height of the red fir bacterium cultivated using the ecosystem of imitative wild environment, quality better rationally make use of cultivation space, industry The commercial value of change is high;Meanwhile, red fir bacterium cultivates base and can repeatedly cultivate red fir bacterium with nutritious liquid prescription, reduces red fir The cultivation cost of bacterium.
Description of the drawings
Fig. 1 is the cultivation structural representation of the fixed culturing frame that horizontal stripe is not fixed in incubator;
Fig. 2 is the cultivation structural representation of the rotatable culturing frame that horizontal stripe is not fixed in incubator;
Fig. 3 is the cultivation structural representation of the fixed culturing frame for having fixed horizontal stripe in incubator;
Fig. 4 is the cultivation structural representation of the rotatable culturing frame for having fixed horizontal stripe in incubator;
Fig. 5 is the schematic appearance fixed culturing frame and cultivate the cultivation row that nutritive layer is combined into;
Fig. 6 is rotatable culturing frame and the schematic appearance for cultivating the cultivation row that nutritive layer is combined into;
Fig. 7 is the schematic appearance of the front view for cultivating nutritive layer and fixed culturing frame combination;
Fig. 8 is the schematic appearance of the front view for cultivating nutritive layer and the combination of rotatable culturing frame.
Fig. 9 is the cross section structure diagram of the front view for cultivating nutritive layer and fixed culturing frame combination;
Figure 10 is the cross section structure diagram of the front view for cultivating nutritive layer and the combination of rotatable culturing frame;
Figure 11 is the structural representation of internal lining pipe and the combination of pallet;
Figure 12 is the structural representation of the combination for fixing turbo- internal lining pipe and pallet;
Figure 13 is the schematic appearance that pedestal is combined with stull;
Figure 14 is the schematic appearance of the top view that pedestal is combined with stull;
Figure 15 is the structural representation of joint tank.
Shown in figure:Pedestal 1, stull 2, protrusion joint 3, clear opening 4, connecting hole 5, upper through hole 6, internal lining pipe 7, convergence hole 8th, nutrition supply chamber 9, via 10, the gasket seal 11 that trackslips, pad holes 12, connecting tube 13, turbine 14, cultivation nutritive layer 15, worm screw 16th, incubator 17, level plate 18, rubber blanket 19, joint tank 20, gas tube orifice 21, clean water outlet 22, passage 23, demarcation strip 24, disappear Venom mouth 25, nutritional solution mouth 26.
Specific embodiment
Efficient green of the present invention cultivates red fir bacterium solid case mainly includes incubator 17, frozen cooling device or cold Gas heat sink, heater, level plate 18, culturing frame, cultivate nutritive layer 15, clean water tank, disinfectant solution tank, nutrition fluid reservoir, Air accumulator;Described culturing frame includes fixed culturing frame and rotatable culturing frame.
Heater and frozen cooling device or cold air heat sink are installed in incubator 17, before incubator 17 Face one side be provided with door, the both sides of incubator 17 and it is disposed behind have vent window, it is laterally uniform in incubator 17 Have levels plate 18, and level plate 18 is fixed on the both sides in incubator 17, or, level plate 18 is arranged on and is fixed in incubator 17 Both sides fixed horizontal stripe on;Bottom in incubator 17 constitutes multi-level cultivation layer, the bottom of incubator 17 with level plate 18 Portion and top are parallel to each other with level plate 18.Uniformly it is placed with above bottom and every secondary plate 18 from level to level in incubator 17 Fixed culturing frame or rotatable culturing frame, on the pallet of fixed culturing frame or on the pallet 12 of rotatable culturing frame training is fixed with Educate nutritive layer 15;Each is fixed culturing frame or rotatable culturing frame and is one and cultivates row, bottom in incubator and each There is multiple cultivation to go on level plate;It is fixed on the connecting tube 13 and five of the end of one end of fixed culturing frame or rotatable culturing frame One port connection of siphunculus, remaining four port of Five-way tube connecting pipe, air accumulator respectively with nutrition fluid reservoir Connecting pipe, the connecting pipe of disinfectant solution tank and clean water tank connecting pipe connection, connection clean water tank, disinfectant solution tank, sky Controlled switch is respectively mounted in gas accumulator, the pipeline of nutrition fluid reservoir.Below per secondary plate 18 from level to level one side and Top in incubator 17 is provided with sprayer unit, electric filament lamp, green light lamp and high intensity flasher, electric filament lamp, green light lamp and The circuit of high intensity flasher is connected with light-illuminating controller, light-illuminating controller control electric filament lamp, green light lamp and height The circulation opening time and circulation shut-in time of the light of intensity flash device.
Described incubator 17 is using one or more in timber, insulation material, plastics, nylon, cement, metal material Material manufacture, creates the insulation housing of the cultivation thing in the supporter and guard box that support level plate 18 or has insulation The housing of provision for thermal insulation;The effect of incubator 17:First, prevent antibacterial from entering to cultivate in nutritive layer 15 and play the work of virus-isolating With two, the loss for making cultivation nutritive layer 15 separate with soil and reducing the nutritional labeling of organic soil.Incubator 17 is being installed When, it is placed on the bracing frame of 5 ° of skewed horizontal loads to 30 ° of gradients, or, it is placed on the gradient for being organized into 5 ° to 30 ° Flooring or ground on.
Described level plate 18 adopts one or more material manufactures in timber, plastics, nylon, cement, metal material, Create the carrier placed fixed culturing frame or rotatable culturing frame and cultivate nutritive layer 15.
Described fixed horizontal stripe adopts one or more material manufactures in timber, plastics, nylon, cement, metal material, The horizontal fixed-length block parallel with the bottom and top of incubator 17 or strip are created, it is same when in incubator 17 The fixed horizontal stripe of individual level it is highly consistent, it is parallel with level plate 18 with the bottom and top that ensure incubator 17.
Described fixed culturing frame includes pedestal 1, stull 2, internal lining pipe 7, pallet 12, rubber blanket 19, connecting tube 13;It is described Rubber blanket be that centre has the Gask-O-Seal in hole;Pallet is fixed with internal lining pipe 7 below the via of the outside of convergence hole 8 12, the convergence hole 8 of the lower end of internal lining pipe 7 is socketed on the protrusion joint 3 above pedestal 1, and rubber blanket 19 is padded in convergence hole 8 Place, the end insertion of connecting tube 13 or the connecting hole 5 of screw-in pedestal 1 end are tightly connected between the upper end of end and protrusion joint 3 It is interior, the internal run-through of upper through hole 6 and the insertion of internal lining pipe 7, clear opening 4 and connecting tube 13 in pedestal 1;Stull 2 is fixed on pedestal 1 Both sides stably fix culturing frame and do not incline to both sides, connecting tube 13 is fixed on the rear and front end of pedestal 1 and pedestal 1 is together in series And culturing frame not rear-inclined forward is stably fixed, the connecting hole 5 of last pedestal 1 of fixed culturing frame one end has plug to block Passage, the connecting tube 13 of last pedestal 1 of the culturing frame other end that is connected is connected with one end of Five-way tube;One fixation Culturing frame has multiple pedestals 1, multiple stulls 2, multiple internal lining pipes 7 and multiple pallets 12.
Described rotatable culturing frame include pedestal 1, stull 2, internal lining pipe 7, pallet 12, turbine 14, worm screw 16, trackslip it is close Packing/11, connecting tube 13;The described gasket seal 11 that trackslips creates the combination that upper and lower surface can mutually trackslip and seal There is through hole pad, the centre of compound gasket;In the end insertion of connecting tube 13 or the connecting hole 5 of screw-in pedestal 1 end, pedestal 1 Interior upper through hole 6 and the insertion of internal lining pipe 7, the internal run-through of clear opening 4 and connecting tube 13;8 sets of the convergence hole of the lower end of internal lining pipe 7 Be connected on the protrusion joint 3 above pedestal 1, the gasket seal 11 that trackslips pad the inner of convergence hole 8 with protrusion joint 3 upper end it Between be tightly connected place;The lower end of the outside of internal lining pipe 7 is fixed with turbine 14 at lower end, with bottom above turbine 14 Via 10 between internal lining pipe 7 on be fixed with pallet 12;Turbine 14 coordinates with worm screw 16, and worm screw 16 can drive turbine 14 to revolve Turn, the axle of worm screw 16 directly with the axle Hard link of adjustable frequency motor or be flexible coupling, or, the axle of worm screw 16 by variator again with The axle connection of common motor;Stull 2 is fixed on the both sides of pedestal 1 and stablizes rotatable culturing frame not to both sides inclination, connecting tube 13 are fixed on the rear and front end of pedestal 1 is together in series pedestal 1 and stable culturing frame not rear-inclined forward, rotatable culturing frame one The connecting hole 5 of last pedestal 1 at end has plug to block passage, connects last pedestal of the rotatable culturing frame other end 1 connecting tube 13 is connected with one end of Five-way tube;One rotatable culturing frame has multiple pedestals 1, multiple stulls 2, multiple liners Pipe 7 and multiple pallets 12.
There is protrusion joint 3 lower sections of less than pedestal above described pedestal 1, the right and left of pedestal 1 can be consolidated Determine stull 2, there is connecting hole 5 front-end and back-end of pedestal 1, the company of the front-end and back-end of the clear opening 4 in pedestal 1 and pedestal 1 The insertion of hole 5 is connect, the upper through hole 6 of and insertion vertical with the clear opening 4 in pedestal 1 is worn out less than the lower sections of protrusion joint of pedestal 3 upper surface, clear opening 4 forms interior threeway with upper through hole 6.
Described stull 2 creates hollow or solid cross-brace frame;Described pedestal 1 and stull 2 are to separate system Assembly after making creates an entirety;Described internal lining pipe 7 creates hollow pipe, the lower end manufacture of internal lining pipe 7 There is convergence hole 8, manufacture around the upper end of internal lining pipe 7 and the internal lining pipe 7 of the above section of convergence hole 8 has via 10, in convergence hole 8 The hollow parts of the internal lining pipe 7 of upper part create nutrition supply chamber 9;Described pallet 12 creates centre hole Flat board or disk, pallet 12 is sleeved on internal lining pipe 7 or pallet 12 creates an entirety with internal lining pipe 7, and pallet 12 is to support The carrier of nutritive layer 15 is cultivated, pallet 12 coordinates with internal lining pipe 7 can fix cultivation nutritive layer 15;Described connecting tube 13 is manufactured Become hollow siphunculus, for conveying nutritional solution, disinfectant solution, clear water and compressed air;Described via 10 is created and allows battalion The hole that nutrient solution, disinfectant solution, clear water and compressed air are passed through, via 10 is used to convey nutritional solution, disinfectant solution, clear water and compressed air To cultivation nutritive layer 15;Described nutrition supply chamber 9 is used to transmit nutritional solution, disinfectant solution, clear water and compressed air at via.
Described cultivation nutritive layer 15 is that red fir bacterium cultivates base, is the part for storing nutritional labeling and the group for cultivating red fir bacterium Synthesis point solid layer, be cultivate red fir bacterium nutritional labeling materials processing become remix after powder together add Folium Pini water Fermentation body afterwards;Described Folium Pini water is the water juice being boiled into Folium Pini;The material of described red fir bacterium culture medium includes:Ramulus Pini, Corn cob, pine forest thin sand and soil, rice husk, Gypsum Fibrosum, white sugar, the ratio of its composition is:5 parts of Ramulus Pini, 2 parts of corn cob, pine forest thin sand and soil 2 Part, 1 part of rice husk, 1 part of Gypsum Fibrosum, 1 part of white sugar.Described cultivation nutritive layer 15 be able to can repeat to cultivate red with nutritious liquid prescription Fir bacterium, and ensure that the quality of the red fir bacterium cultivated.
Described clean water tank is the tank body for containing clear water;Described disinfectant solution tank is the tank body for containing disinfectant solution, described Disinfectant solution is lime water;Described nutrition fluid reservoir is the tank body for containing nutritional solution;Described air accumulator is storage pressure The tank body of contracting air, the air in tank has pressure;Described nutrition fluid reservoir, air accumulator, disinfectant solution tank and clean water tank are The separate component being individually separated, or, air accumulator is combined into a multi-cavity with nutrition fluid reservoir, disinfectant solution tank and clean water tank United joint tank 20;It is united that air accumulator is combined into a multi-cavity with nutrition fluid reservoir, disinfectant solution tank and clean water tank During joint tank 20, the inside for combining tank 20 is separated into nutrition sap cavity, sterilization sap cavity and clear water chamber three respectively by demarcation strip 24 Self-packing chamber, in the connection nutrition sap cavity of passage 23, three chambers of sterilization sap cavity and clear water chamber on the top of demarcation strip 24, nutritional solution It is partly the part of storing compressed air above chamber, sterilization sap cavity and clear water chamber three chambers, nutrition sap cavity, sterilization sap cavity and clear It is partly the part for storing nutritional solution, disinfectant solution and clear water below three chambers of water cavity, the conveying battalion being connected with nutrition supply chamber 9 The clean water outlet 22 of the pipeline of the nutritional solution mouth 26, the disinfectant solution mouth 25 of the pipeline of conveying disinfectant solution and conveying clear water of the pipeline of nutrient solution All in the bottom of the internal cavities of joint tank 20, the gas tube orifice 21 of the compressed air piping being connected with nutrition supply chamber 9 is in joint The top of the internal cavities of tank 20;Compressed air in joint tank 20 serves pump above nutritional solution, disinfectant solution and clear water Effect and reduce infusion pump.
In order that turn out the red fir bacterium for coming yield is higher, quality more preferably, will be to training in the incubation of red fir bacterium Educate conveying nutritional solution in nutritive layer 15;Described nutritional solution is by the slurry broken into after Testa Tritici, Folium Pini, Semen Allii Tuberosi, Semen Pini, Gypsum Fibrosum mixing The emulsion of Jing everfermentations after a small amount of white sugar and potassium dihydrogen phosphate mix homogeneously is added, then the emulsion after fermentation is filtered off slag Obtained from be without slag emulsion, the ratio of nutritional solution material composition:15 parts of Testa Tritici, 6 parts of Folium Pini, 3 parts of Semen Allii Tuberosi, 2 parts of Semen Pini, white sugar 1 part, 1 part of Gypsum Fibrosum, 1 part of potassium dihydrogen phosphate.Biphosphate potassium composition is added in nutritional solution, make the nutrition of red fir bacterium culture medium into Divide and keep permanent, and the fast-growth of red fir bacterium can be promoted, make the fresh and tender of red fir bacterium that grow out;Pine is added in nutritional solution Pin composition, the nutritional labeling for making red fir bacterium culture medium keeps permanent, and can promote the fast-growth of red fir bacterium, makes to grow out Red fir bacterium it is bright-colored and fresh and tender;Gypsum component is added in nutritional solution, the clean taste of red fir bacterium can be made fine and smooth;In battalion Testa Tritici composition is added in nutrient solution, the fast-growth of red fir bacterium and the fair and tender degree of the red fir bacterium of increase can be promoted;Add in nutritional solution Enter Semen Allii Tuberosi composition, the fast-growth of red fir bacterium can be promoted, increase the nutritional labeling and exquisiteness of red fir bacterium, make the mouth of red fir bacterium Feel soft and smooth exquisiteness.
In order to prevent to cultivating in nutritive layer 15 after conveying nutritional solution, the moisture cultivated in nutritive layer 15 is too high, adopts It is dried two methods to reduce the moisture cultivated in nutritive layer 15 with inclining dewatering and drying;Described inclination dewatering is to train Educate case 17 and level plate 18 inclines certain angle;It is from Jing in nutrition supply chamber 9 by compressed air that described blowing is dried Via 10 is blowed in cultivation nutritive layer 15, and air takes cultivation nutritive layer 15 out of the unnecessary moisture in cultivation nutritive layer 15 Outward.
In order to increase cultivation face, placed using two inclined-planes and cultivate nutritive layer 15 to increase cultivation face.
In order that red fir bacterium has wild taste, fog is sprayed using sprayer unit, make red fir bacterium obtain rain and dew;Using white Vehement lamp makes red fir bacterium obtain wild environment with the flash irradiation of the moment of the circulation irradiation and high intensity flasher of green light lamp Growth conditionss, the ability that the ring grain ring on the cap of red fir bacterium can be promoted to form and strengthen the absorption nutritional labeling of red fir bacterium.
In order that the red fir bacterium vaccine cultivated on nutritive layer 15 can uniformly receive the irradiation of light, in irradiation light When, the power supply for driving worm screw 16 is first connected, make worm screw 16 drive turbine 14 to rotate, then the switch of light is opened, make light Light is radiated on the vaccine cultivated on nutritive layer 15 of rotation.
The material of the inoculation bacterium bag of red fir bacterium is mixed using addition white sugar after the crushing of pine sawdust, Testa oryzae or Testa Tritici and Gypsum Fibrosum powder It is even, add Folium Pini water mix it is wet after form, the ratio of its composition is pine sawdust 78%, Testa oryzae or Testa Tritici 20%, white sugar 1%, stone Cream powder 1%.In order that the bacterium bag of postvaccinal red fir bacterium is easy to be trapped among on the face of the cultivation nutritive layer 15 of the outside of internal lining pipe 7, institute The inoculation bacterium bag stated makes the bacterium bag of rectangular thin type.Enclose the training being taped against on internal lining pipe 7 after postvaccinal bacterium bag is cut off again Educate on the surface of nutritive layer 15, enclose and be taped against on the surface for cultivating nutritive layer 15 after fixation with compressed air from nutrition supply chamber 9 Blow in cultivation nutritive layer 15 through via 10, the mycelia for making inoculation rocks with compressed air, reach wake-up mycelia and emerge and grow The purpose of bacterium;Reduce tradition cultivate manually scratching during red fir bacterium wrap wake up mycelia emerge and long bacterium program, together When decrease the waste of the material for manually scratching the red fir bacterium culture medium drawn out during bag.
In order that red fir bacterium culture medium fixation and allows inoculum bag in red fir bacterium culture medium fix tightly on internal lining pipe 7 Gu, red fir bacterium culture medium is held using pallet and is fixed on internal lining pipe 7 with interior fixed net, using outer fixed net by inoculum bag It is fixed on red fir bacterium culture medium.
Nurturing an environment in described incubator 17 is to imitate the nurturing an environment under wild natural ecological environment, is adopted Arrange the measure of insulation and cooling in incubator 17 to realize temperature control;Season when cultivating red fir bacterium without following cultivation advises Rule, can start at any time the imitative wild nurturing an environment in incubator 17 and enter in arbitrary season and arbitrary natural environment Row ecological organic is cultivated;Collect imitative Wild ecological environment with organic cultivation and stereo cultivation in each incubator 17, realize The automated management that organic three-dimensional agriculture is cultivated.
Described frozen cooling device and cold air heat sink is heat sink, using freezing cooling water circulation, or, adopt Lowered the temperature with circulating cold air, or, lowered the temperature using compressor cooling;Described heater be using electric energy heating heating tube or Steam circulation heating tube or hot-air blower, are heat risers.
In order to improve the quality of red fir bacterium and the growth beneficial to red fir bacterium, pallet 12 and internal lining pipe 7 adopt during fabrication nothing The plastics or glass steel or stainless steel manufacture of poison.
In order that nutritional solution can be input to cultivation nutritive layer 15 in, nutrition fluid reservoir, disinfectant solution tank and clean water tank with The internal cavity of air accumulator creates the joint cavity of an entirety, using inflating pump that compressed air input joint is empty Intracavity, the pressure instead work of infusion pump that compressed air is produced;Or, by nutrition fluid reservoir, clean water tank and disinfectant solution tank Above incubator 17;Or, in the tank of nutrition fluid reservoir, clean water tank and disinfectant solution tank or in connection nutrition liquid storage Infusion pump is installed in the pipeline of tank, clean water tank and disinfectant solution tank.
In order that have air pressure in air accumulator or in joint tank, using inflating pump gases at high pressure input air accumulator In or joint tank in.
In order to prevent virus and can sterilize to cultivating nutritive layer 15, using 2-3% lime water to cultivating battalion Foster layer 15 carries out disinfection sterilization;The method of its disinfection is that 2-3% lime water is put in disinfectant solution tank, by pipeline Is input on red fir bacterium cultivation base to be planted with nutrition supply chamber 9 and red fir bacterium cultivation base is carried out disinfection sterilization.
When cultivating red fir bacterium in the incubator 17, first by the inoculation bacterium bag of red fir bacterium send into freezing it is indoor subzero 15 DEG C with Under cold air freeze 10 hours, reach freezing sterilizing purpose;After sterilizing, the inoculation bacterium bag after defrosting is sent into 23 DEG C extremely In 25 DEG C of aseptic inoculating room, put vaccine into inoculation bacterium bag and be inoculated with, complete within 24 hours inoculation;Afterwards, will be postvaccinal Bacterium bag is sent into 25 DEG C to 32 DEG C of interior and is cultivated, and after 40 to 45 days, the mycelia that being inoculated with has white in bacterium bag is paved with bacterium bag i.e. For mycelia culture success;Afterwards, the inoculation bacterium bag for covering with mycelia is put in cultivation box and red fir bacterium is cultivated.
Before the inoculation bacterium bag for covering with mycelia is put in cultivation box, first with 2-3% lime water to cultivating nutritive layer 15 carry out disinfection sterilization;The method of its disinfection is that 2-3% lime water is put in disinfectant solution tank, by pipeline and battalion Foster feeding chamber 9 is input on red fir bacterium cultivation base to be planted and red fir bacterium cultivation base is carried out disinfection sterilization, the input of lime water Measure and cultivate nutritive layer 15 for standard just to soak, cultivate the switch that sterilizing path is closed after nutritive layer 15 soaks;Sterilization one day Afterwards, the switch compressed air for opening compressed air is dried up after red fir bacterium cultivation base, closes the switch of compressed air;Afterwards, will Temperature in incubator 17 is adjusted to 25 DEG C to 32 DEG C, when temperature be less than 25 DEG C when with heating devices heat be adjusted to 25 DEG C with On, it is adjusted to less than 32 DEG C with frozen cooling device or the cooling of cold air heat sink when the temperature in cultivation box is higher than 32 DEG C;It Afterwards, enclose fixed on the surface of the cultivation nutritive layer 15 being taped against around internal lining pipe 7 after the inoculation bacterium bag for covering with mycelia is cut off again; Cover with mycelia inoculation bacterium bag enclose be taped against cultivate nutritive layer 15 surface on after mycelia is carried out scratching within first day disturb, be commonly called as scratching Bag.
Scratch when disturbing mycelia, open the switch that airport is connected with nutrition supply chamber 9, compressed air is into nutrition supply chamber 9 Interior entrance after cultivation nutritive layer 15 by via 10 blows again mycelia, rocks mycelia three times, rocks bacterium every time by compressed air Silk closes the air inlet switch of compressed air after 10 to 20 minutes, mycelia can be waken up when rocking mycelia, makes mycelia emerge and long bacterium, Stop scratching after one day disturbing mycelia;Mycelia is grown after vaccine, and the root of vaccine is deeply cultivated in nutritive layer 15 and absorbs nutrition and grow up, this When, to the supply nutrition of nutritive layer 15 is cultivated and to carry out light-illuminating;The mode of supply nutrition adopts nutritional solution replenishment, goes out bacterium The initial stage of Seedling, supplement was once, supplement once daily into after bacterium per 2 days;During extra-nutrition, nutrition fluid reservoir and nutrition supply are opened The connecting valve in chamber 9, nutritional solution is entered through via 10 after entering in nutrition supply chamber 9 and cultivated in nutritive layer 15, waits to cultivate battalion There is moistening and closing switch during color burn in the surface of foster layer 15, stops conveying nutritional solution;Fixed training is placed with incubator When educating the irradiation light light of frame, the switch of light is directly opened;When the irradiation light light of rotatable culturing frame is placed with incubator, The power supply of the motor for driving the rotation of worm screw 16 is first connected, makes worm screw 16 drive turbine 14 to open the switch of light after rotating again, And the light of light is radiated on the cultivation nutritive layer 15 of rotation, make that the vaccine on cultivation nutritive layer 15 obtains then is efficient Uniformly, cut-out when closing light drives the power supply of the motor of the rotation of worm screw 16;During irradiation light light, opening for lamp dimmer is opened Close, green glow light irradiation used 5 minutes again after being irradiated 1 minute with electric filament lamp, electric filament lamp is closed after replacing irradiation with green light lamp 2 hours, Interval replaces irradiation with green light lamp with electric filament lamp again after 1 hour, opens high intensity flash of light dress when green light lamp is opened every 9 hours Put and cultivate nutritive layer 3 seconds with high intensity flash irradiation, so ceaselessly illumination till Mining harvests red fir bacterium, makes lamp for circulation The light of light is radiated at cultivating on nutritive layer 15 and making the light of vaccine acquisition for rotation;The circulation irradiation of different light makes red fir Bacterium obtains the growth conditionss of wild environment, and the ring grain ring on the cap of red fir bacterium can be promoted to form and strengthen the absorption of red fir bacterium The ability of nutritional labeling, makes cap hypertrophy, bacterium bar sturdy.
In the cultivating process of red fir bacterium, at least daily with the compressed air in air accumulator by nutrition supply chamber 9 blow one to secondary to nutritive layer 15 is cultivated, and at least 1 minute every time, to increase the ventilation of organic soil the root of red fir bacterium are made Portion's respiratory ventilation in organic soil, meanwhile, Jing often to open vent window to ventilation in incubator 17, it is ensured that incubator 17 Interior air is fresh, and slightly sprays a spray water with sprayer unit daily and make red fir bacterium obtain the dew of wild environment Water;The red fir bacterium cultivated in incubator 17 is ripe successively, when the bacteria cover diameter of red fir bacterium reaches 8 to 10cm, cap Color is purplish red and bright-coloured, ring grain substantially, bacterium bar is mellow and full and appear yellowish pink, whole red fir bacterium is suffused an exquisite fragrance all around, be now red fir bacterium into Ripe mark, the ripe red fir bacterium of Mining harvests;The strain implantation of red fir bacterium to be cultivated and can again bear strain after nutritive layer 15, is added Electric filament lamp cultivates nutritive layer 15 with the illumination of green light lamp and the lasting supplementary of nutritional solution, and daily Mining is received to enable red fir bacterium, is continued Mining was received for up to 18 months, completed the green organic stereo cultivation of a round of the red fir bacterium of high-quality in incubator 17 Overall process.
Red fir bacterium is cultivated using the equipment in the three-dimensional case and three-dimensional case of the present invention, even if by the composition of nutritional solution and red fir Bacterium cultivates the composition of base and is changed, and falls within protection scope of the present invention.

Claims (6)

1. a kind of efficient green cultivates red fir bacterium solid case, it is characterised in that:Described efficient green cultivates red fir bacterium solid case Including incubator, frozen cooling device or cold air heat sink, heater, level plate, fixed culturing frame, cultivate nutritive layer, Clean water tank, disinfectant solution tank, nutrition fluid reservoir, air accumulator;
Heater and frozen cooling device or cold air heat sink are installed in incubator, are simultaneously set before incubator Be equipped with door, the both sides of incubator and it is disposed behind have vent window, the laterally uniform plate that has levels in incubator, level Plate is fixed on the both sides in incubator, or, level plate is arranged on and is fixed on the fixed horizontal stripe of the both sides in incubator;Cultivate Bottom in case constitutes multi-level cultivation layer with level plate, and bottom and top and the level plate of incubator are parallel to each other;In training Educate and be uniformly placed with fixed culturing frame above the bottom in case and every plate secondary from level to level, it is fixed on the pallet of fixed culturing frame There is cultivation nutritive layer;It is a cultivation row that each fixes culturing frame, has many on the bottom and each level plate in incubator It is individual to cultivate row;The connecting tube of end for being fixed on one end of fixed culturing frame is connected with a port of Five-way tube, Five-way tube Remaining four port connecting pipe, the connecting pipe of air accumulator, connecting tube of disinfectant solution tank respectively with nutrition fluid reservoir The connecting pipe connection of road and clean water tank, in connection clean water tank, disinfectant solution tank, air accumulator, the pipeline of nutrition fluid reservoir It is respectively mounted controlled switch;Top in per one side and incubator below plate secondary from level to level be provided with sprayer unit, Electric filament lamp, green light lamp and high intensity flasher, electric filament lamp, the circuit of green light lamp and high intensity flasher and light-illuminating control Device connection processed, the circulation opening time of the light of light-illuminating controller control electric filament lamp, green light lamp and high intensity flasher With the circulation shut-in time;
Described incubator is using one or more the material systems in timber, insulation material, plastics, nylon, cement, metal material Make, create the insulation housing of the cultivation thing in the supporter and guard box that support level plate or have heat insulation measure Housing;The effect of incubator:First, prevent antibacterial from entering to cultivate in nutritive layer and play a part of virus-isolating, two, make cultivation Nutritive layer is separated with soil and reduces the loss of the nutritional labeling of organic soil;Incubator when mounted, has been placed on 5 ° to 30 ° On the bracing frame of the skewed horizontal load of gradient, or, it is placed on and is organized on the flooring of 5 ° to 30 ° of gradient or ground;
Described level plate is manufactured into using one or more material manufactures in timber, plastics, nylon, cement, metal material To place fixed culturing frame and cultivating the carrier of nutritive layer;
Described fixed horizontal stripe is using one or more material manufactures in timber, plastics, nylon, cement, metal material, manufacture Become the horizontal fixed-length block parallel with the bottom of incubator and top or strip, same level when in incubator The highly consistent of horizontal stripe is fixed, it is parallel with level plate with the bottom and top that ensure incubator;
Described fixed culturing frame includes pedestal, stull, internal lining pipe, pallet, rubber blanket, connecting tube;During described rubber blanket is Between have the Gask-O-Seal in hole;Pallet is fixed with the internal lining pipe below via outside convergence hole, the lower end of internal lining pipe Convergence hole is socketed on the protrusion joint of impact faces, and rubber blanket pad is close between inner and protrusion joint the upper end of convergence hole Envelope junction, the end insertion of connecting tube or screws in the connecting hole of base ends, upper through hole in pedestal and internal lining pipe insertion, The internal run-through of clear opening and connecting tube;Stull is fixed on the both sides of pedestal and stably fixes culturing frame not to both sides inclination, connection Pipe is fixed on the rear and front end of pedestal and pedestal is together in series and culturing frame not rear-inclined forward, fixed culturing frame one is stably fixed The connecting hole of last pedestal at end has plug to block passage, the company of last pedestal of the culturing frame other end that is connected Adapter is connected with one end of Five-way tube;One fixed culturing frame has multiple pedestals, multiple stulls, multiple internal lining pipes and multiple supports Disk;
Have above described pedestal less than the lower sections of protrusion joint of pedestal, the right and left of pedestal can fix stull, There is connecting hole the front-end and back-end of pedestal, the connecting hole insertion of the front-end and back-end of the clear opening in pedestal and pedestal, with base Clear opening in seat is vertical and upper through hole of insertion wears out the upper surface of protrusion joint lower sections of less than pedestal, clear opening with Upper through hole forms interior threeway;
Described stull creates hollow or solid cross-brace frame;Described pedestal and stull be it is separately manufactured after group Zoarium creates an entirety;Described internal lining pipe creates hollow pipe, and the lower end manufacture of internal lining pipe has convergence hole, interior Manufacture around the upper end of bushing pipe and the internal lining pipe of convergence hole above section has via, in the internal lining pipe of convergence hole upper part Hollow parts create nutrition supply chamber;Described pallet creates centre the flat board or disk in hole, and pallet is sleeved on interior On bushing pipe or pallet and internal lining pipe create an entirety, pallet is to support the carrier for cultivating nutritive layer, pallet and internal lining pipe Cooperation can fix cultivation nutritive layer;Described connecting tube creates hollow siphunculus, for convey nutritional solution, disinfectant solution, Clear water and compressed air;Described via creates the hole for allowing nutritional solution, disinfectant solution, clear water and compressed air to pass through, via For conveying nutritional solution, disinfectant solution, clear water and compressed air to cultivation nutritive layer;Described nutrition supply chamber is used to transmit nutrition Liquid, disinfectant solution, clear water and compressed air are at via;
Described cultivation nutritive layer is that red fir bacterium cultivates base, is the part for storing nutritional labeling and the composition for cultivating red fir bacterium Solid layer, be cultivate red fir bacterium nutritional labeling materials processing become remix after powder together add Folium Pini water after send out Ferment body;Described Folium Pini water is the water juice being boiled into Folium Pini;The material of described red fir bacterium culture medium includes:Ramulus Pini, corn cob, Pine forest thin sand and soil, rice husk, Gypsum Fibrosum, white sugar;Described cultivation nutritive layer be able to can repeat to cultivate red fir with nutritious liquid prescription Bacterium, and ensure that the quality of the red fir bacterium cultivated;
Described clean water tank is the tank body for containing clear water;Described disinfectant solution tank be contain disinfectant solution tank body, described sterilization Liquid is lime water;Described nutrition fluid reservoir is the tank body for containing nutritional solution;Described air accumulator is that storage compression is empty The tank body of gas, the air in tank has pressure;Described nutrition fluid reservoir, air accumulator, disinfectant solution tank and clean water tank are respective Separate separate component, or, air accumulator is combined into a multi-cavity and combines with nutrition fluid reservoir, disinfectant solution tank and clean water tank Joint tank;Air accumulator is combined into a multi-cavity and combines tank in combination with nutrition fluid reservoir, disinfectant solution tank and clean water tank When, combine the inside of tank becomes nutrition sap cavity, sterilization sap cavity and each self-packing chamber of clear water chamber three by divides, The passage connection nutrition sap cavity on demarcation strip top, three chambers of sterilization sap cavity and clear water chamber, nutrition sap cavity, sterilization sap cavity and clear water It is partly the part of storing compressed air above the chamber of three, chamber, below nutrition sap cavity, three chambers of sterilization sap cavity and clear water chamber Part is the part for storing nutritional solution, disinfectant solution and clear water, the nutrition of the pipeline of the conveying nutritional solution being connected with nutrition supply chamber The clean water outlet of the pipeline of liquid mouth, the disinfectant solution mouth of the pipeline of conveying disinfectant solution and conveying clear water is all in the internal cavities for combining tank Bottom, the gas tube orifice of the compressed air piping being connected with nutrition supply chamber is at the top of the internal cavities for combining tank;In joint tank Compressed air the effect of pump is served above nutritional solution, disinfectant solution and clear water and infusion pump is reduced;
In order that turn out the red fir bacterium for coming yield is higher, quality more preferably, will be to cultivating battalion in the incubation of red fir bacterium Conveying nutritional solution in foster layer;Described nutritional solution is added by the slurry broken into after Testa Tritici, Folium Pini, Semen Allii Tuberosi, Semen Pini, Gypsum Fibrosum mixing The emulsion of Jing everfermentations after a small amount of white sugar and potassium dihydrogen phosphate mix homogeneously, then the emulsion after fermentation is filtered off slag and is obtained Biphosphate potassium composition is added in nutritional solution without slag emulsion, the nutritional labeling for making red fir bacterium culture medium keeps permanent, and energy Enough promote the fast-growth of red fir bacterium, make the fresh and tender of red fir bacterium that grow out;Folium Pini composition is added in nutritional solution, red fir bacterium is made The nutritional labeling of culture medium keeps permanent, and can promote the fast-growth of red fir bacterium, and the color of the red fir bacterium that makes to grow out is fresh It is gorgeous and fresh and tender;Gypsum component is added in nutritional solution, the clean taste of red fir bacterium can be made fine and smooth;Testa Tritici is added in nutritional solution Composition, the fast-growth that red fir bacterium can be promoted and the fair and tender degree for increasing red fir bacterium;Semen Allii Tuberosi composition is added in nutritional solution, can Promote the fast-growth of red fir bacterium, increase the nutritional labeling and exquisiteness of red fir bacterium, make the smooth mouth feel of red fir bacterium fine and smooth;
In order to increase cultivation face, placed using two inclined-planes and cultivate nutritive layer to increase cultivation face;
In order that red fir bacterium has wild taste, fog is sprayed using sprayer unit, make red fir bacterium obtain rain and dew;Using electric filament lamp Red fir bacterium is set to obtain the growth of wild environment with the flash irradiation of the moment of the circulation irradiation and high intensity flasher of green light lamp Condition, the ability that the ring grain ring on the cap of red fir bacterium can be promoted to form and strengthen the absorption nutritional labeling of red fir bacterium;
In order that the red fir bacterium vaccine cultivated on nutritive layer can uniformly receive the irradiation of light, and in irradiation light light, be first The power supply for driving worm screw is connected, makes worm screw drive turbine to rotate, then open the switch of light, the light for making light is radiated at rotation Cultivate nutritive layer on vaccine on;
The material of the inoculation bacterium bag of red fir bacterium is mixed thoroughly using addition white sugar after the crushing of pine sawdust, Testa oryzae or Testa Tritici and Gypsum Fibrosum powder, then Add Folium Pini water mix it is wet after form, the ratio of its composition is pine sawdust 78%, Testa oryzae or Testa Tritici 20%, white sugar 1%, Gypsum Fibrosum powder 1%;In order that the bacterium bag of postvaccinal red fir bacterium is easy to be trapped among on the face of the cultivation nutritive layer outside internal lining pipe, described inoculation Bacterium bag makes the bacterium bag of rectangular thin type;Enclose the cultivation nutritive layer being taped against on internal lining pipe after postvaccinal bacterium bag is cut off again On surface, enclose be taped against it is fixed on the surface for cultivating nutritive layer after blow to cultivation through via from nutrition supply chamber with compressed air In nutritive layer, the mycelia for making inoculation rocks with compressed air, reach wake up mycelia emerge and long bacterium purpose;Reduce traditional training Educate manually scratching during red fir bacterium wrap wake up mycelia emerge and long bacterium program, while decreasing manually scratch bag when institute The waste of the material of the red fir bacterium culture medium drawn out;
In order that red fir bacterium culture medium fixation and allows inoculum bag in red fir bacterium culture medium fixation on internal lining pipe, adopt Red fir bacterium culture medium is held with pallet and be fixed on internal lining pipe with interior fixed net, be fixed on inoculum bag using outer fixed net On red fir bacterium culture medium;
Nurturing an environment in described incubator is to imitate the nurturing an environment under wild natural ecological environment, using in incubator It is interior to arrange the measure of insulation and cooling to realize temperature control;It is in office without following the Seasonal Regularity of cultivation when cultivating red fir bacterium Can start the imitative wild nurturing an environment in incubator at any time in the season of meaning and arbitrary natural environment and carry out organic life State is cultivated;Collect imitative Wild ecological environment with organic cultivation and stereo cultivation in each incubator, realize organic three-dimensional agriculture The automated management that industry is cultivated;
Described frozen cooling device and cold air heat sink is heat sink, using freezing cooling water circulation, or, using cold Gas circulation temperature lowering, or, lowered the temperature using compressor cooling;Described heater is using the heating tube or steam of electric energy heating Circulating-heating pipe or hot-air blower, are heat risers;
In order that nutritional solution can be input in cultivation nutritive layer, nutrition fluid reservoir, disinfectant solution tank and clean water tank and air are stored up The internal cavity of pressure tank creates the joint cavity of an entirety, and compressed air is input in joint cavity using inflating pump, The pressure instead work of infusion pump that compressed air is produced;Or, nutrition fluid reservoir, clean water tank and disinfectant solution tank are arranged on Above incubator;Or, in the tank of nutrition fluid reservoir, clean water tank and disinfectant solution tank or in connection nutrition fluid reservoir, clear water Infusion pump is installed in the pipeline of tank and disinfectant solution tank.
2. a kind of efficient green as claimed in claim 1 cultivates red fir bacterium solid case, the method for the red fir bacterium of cultivation in its case, its It is characterised by:When red fir bacterium is cultivated in incubator, first by the inoculation bacterium bag of red fir bacterium send into freezing it is indoor subzero 15 DEG C with Under cold air freeze 10 hours, reach freezing sterilizing purpose;After sterilizing, the inoculation bacterium bag after defrosting is sent into 23 DEG C extremely In 25 DEG C of aseptic inoculating room, put vaccine into inoculation bacterium bag and be inoculated with, complete within 24 hours inoculation;Afterwards, will be postvaccinal Bacterium bag is sent into 25 DEG C to 32 DEG C of interior and is cultivated, and after 40 to 45 days, the mycelia that being inoculated with has white in bacterium bag is paved with bacterium bag i.e. For mycelia culture success;Afterwards, the inoculation bacterium bag for covering with mycelia is put in cultivation box and red fir bacterium is cultivated;
Before the inoculation bacterium bag for covering with mycelia is put in cultivation box, first carried out to cultivating nutritive layer with 2-3% lime water Disinfection;The method of its disinfection is that 2-3% lime water is put in disinfectant solution tank, by pipeline and nutrition supply Chamber is input on red fir bacterium cultivation base to be planted and red fir bacterium cultivation base is carried out disinfection sterilization, and the input quantity of lime water is for just It is standard to soak cultivation nutritive layer, cultivates the switch that sterilizing path is closed after nutritive layer soaks;After sterilization one day, compression is opened empty The switch compressed air of gas dries up red fir bacterium and cultivates the switch that compressed air is closed after base;Afterwards, by the temperature in incubator 25 DEG C to 32 DEG C are adjusted to, more than 25 DEG C are adjusted to heating devices heat when temperature is less than 25 DEG C, the temperature in cultivation box Less than 32 DEG C are adjusted to during higher than 32 DEG C with frozen cooling device or the cooling of cold air heat sink;Afterwards, connecing for mycelia will be covered with Plant fixed on the surface for enclosing the cultivation nutritive layer being taped against around internal lining pipe after bacterium bag is cut off again;Enclose in the inoculation bacterium bag for covering with mycelia Be taped against cultivate nutritive layer surface on after mycelia is carried out scratching within first day disturb, be commonly called as scratching bag;
Scratch when disturbing mycelia, open the switch that airport is connected with nutrition supply chamber, compressed air passes through into nutrition supply intracavity Via enters to cultivate blow mycelia again after nutritive layer, rocks mycelia three times, rocks 10 to 20 points of mycelia every time by compressed air The air inlet switch of compressed air is closed after clock, mycelia can be waken up when rocking mycelia, make mycelia emerge and long bacterium, stopped after one day Scratch and disturb mycelia;Mycelia is grown after vaccine, and the root of vaccine is deeply cultivated in nutritive layer and absorbs nutrition and grow up, now, be to cultivation Nutritive layer supplies nutrition and carries out light-illuminating;The mode of supply nutrition adopts nutritional solution replenishment, goes out the initial stage of vaccine per 2 days Supplement once, supplement once daily into after bacterium;During extra-nutrition, the connecting valve of nutrition fluid reservoir and nutrition supply chamber is opened, Nutritional solution is entered to be entered through via after nutrition supply intracavity and cultivated in nutritive layer, the surface of nutritive layer to be cultivated occur moistening and Closing switch during color burn, stops conveying nutritional solution;During irradiation light light, the switch of lamp dimmer is opened, use incandescent bulb Green glow light irradiation is used after penetrating 1 minute 5 minutes again, electric filament lamp is closed after replacing irradiation with green light lamp 2 hours, after being spaced 1 hour again Replace irradiation with green light lamp with electric filament lamp, opened high intensity flasher when green light lamp is opened every 9 hours and dodged with high intensity Light irradiation cultivates nutritive layer 3 seconds, and till Mining harvests red fir bacterium, the light for making light irradiates so to circulate ceaselessly illumination The light on nutritive layer is cultivated obtaining vaccine;The circulation irradiation of different light makes red fir bacterium obtain the growth of wild environment Condition, the ability that the ring grain ring on the cap of red fir bacterium can be promoted to form and strengthen the absorption nutritional labeling of red fir bacterium, makes bacterium Lid is loose, bacterium bar is sturdy;
In the cultivating process of red fir bacterium, at least given by nutrition supply chamber with the compressed air in air accumulator daily and trained Educate nutritive layer and blow one to secondary, at least 1 minute every time, the root of red fir bacterium is made organic to increase the ventilation of organic soil Respiratory ventilation in soil, meanwhile, Jing often to open vent window to ventilation in incubator, it is ensured that the air in incubator is new It is fresh, and slightly spray a spray water with sprayer unit daily and make red fir bacterium obtain the dew of wild environment;In incubator The red fir bacterium of interior cultivation is ripe successively, and when the bacteria cover diameter of red fir bacterium reaches 8 to 10cm, the color of cap is purplish red and fresh Gorgeous, ring grain substantially, bacterium bar is mellow and full and appear yellowish pink, whole red fir bacterium is suffused an exquisite fragrance all around, and is now the ripe mark of red fir bacterium, and Mining is received Ripe red fir bacterium;The strain implantation of red fir bacterium to be cultivated and can again bear strain after nutritive layer, adds electric filament lamp and green light lamp Illumination and the lasting supplementary of nutritional solution cultivate nutritive layer, daily Mining is received to enable red fir bacterium, is continued Mining and is received for up to 18 Month, complete the overall process of the green organic stereo cultivation of a round of the red fir bacterium of high-quality in incubator.
3. efficient green according to claim 1 cultivates red fir bacterium solid case, it is characterised in that:In order to prevent seeking to cultivating In foster layer after conveying nutritional solution, the moisture cultivated in nutritive layer is too high, employs inclination dewatering and blowing is dried two kinds of sides Method come reduce cultivate nutritive layer in moisture;Described inclination dewatering is that incubator and level plate are inclined into certain angle;Institute The blowing stated is dried to be blowed in cultivation nutritive layer through via from nutrition supply chamber by compressed air, and air is cultivation battalion Unnecessary moisture in foster layer is taken out of outside cultivation nutritive layer.
4. efficient green according to claim 1 cultivates red fir bacterium solid case, it is characterised in that:In order to improve red fir bacterium Quality and the growth beneficial to red fir bacterium, pallet and internal lining pipe are during fabrication using nontoxic plastics or glass steel or stainless steel system Make;
In order that have air pressure in air accumulator or in joint tank, using inflating pump in gases at high pressure input air accumulator or In joint tank.
5. efficient green according to claim 1 cultivates red fir bacterium solid case, it is characterised in that:In order to prevent virus and energy It is enough to sterilize to cultivating nutritive layer, carried out disinfection sterilization to cultivating nutritive layer using 2-3% lime water;Its disinfection Method be that 2-3% lime water is put in disinfectant solution tank, red fir to be planted is input to by pipeline and nutrition supply chamber Bacterium is cultivated and cultivates base to red fir bacterium on base and carry out disinfection sterilization.
6. efficient green according to claim 1 cultivates red fir bacterium solid case, it is characterised in that:Described efficient green training Red fir bacterium solid case is educated, is adapted to the red fir bacterium of cultivation of the small-scale red fir bacterium cultivation of family and industrialization, be easy to scale to manage, Red fir bacterium is cultivated using the ecosystem of imitative wild environment, red fir bacterium cultivates base and can repeatedly cultivate red fir with nutritious liquid prescription Bacterium;
Red fir bacterium is cultivated using the equipment in three-dimensional case and three-dimensional case, additionally it is possible to which the composition and red fir bacterium for changing nutritional solution cultivates base Composition.
CN201611011365.XA 2016-11-17 2016-11-17 Three-dimensional box for efficient and environment-friendly culture of lactarius deliciosus and in-box culture method for lactarius deliciosus Pending CN106576893A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111165266A (en) * 2020-01-08 2020-05-19 华南农业大学 Method for continuously expanding propagation of arbuscular mycorrhizal fungal spores through compartment culture

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111165266A (en) * 2020-01-08 2020-05-19 华南农业大学 Method for continuously expanding propagation of arbuscular mycorrhizal fungal spores through compartment culture

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