CN106574298A - 用于样品分析的方法和组合物 - Google Patents
用于样品分析的方法和组合物 Download PDFInfo
- Publication number
- CN106574298A CN106574298A CN201580034146.0A CN201580034146A CN106574298A CN 106574298 A CN106574298 A CN 106574298A CN 201580034146 A CN201580034146 A CN 201580034146A CN 106574298 A CN106574298 A CN 106574298A
- Authority
- CN
- China
- Prior art keywords
- read
- less
- methods
- sequence
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
- C12Q2535/122—Massive parallel sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/159—Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Ecology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462017580P | 2014-06-26 | 2014-06-26 | |
| US62/017,580 | 2014-06-26 | ||
| US201462063870P | 2014-10-14 | 2014-10-14 | |
| US62/063,870 | 2014-10-14 | ||
| PCT/US2015/038143 WO2015200871A1 (en) | 2014-06-26 | 2015-06-26 | Methods and compositions for sample analysis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106574298A true CN106574298A (zh) | 2017-04-19 |
Family
ID=54929861
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201580034146.0A Pending CN106574298A (zh) | 2014-06-26 | 2015-06-26 | 用于样品分析的方法和组合物 |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US20150376605A1 (enExample) |
| EP (1) | EP3161161A4 (enExample) |
| JP (1) | JP2017523774A (enExample) |
| KR (1) | KR20170023011A (enExample) |
| CN (1) | CN106574298A (enExample) |
| AU (1) | AU2015279619A1 (enExample) |
| CA (1) | CA2953473A1 (enExample) |
| IL (1) | IL249618A0 (enExample) |
| MX (1) | MX2016016898A (enExample) |
| WO (1) | WO2015200871A1 (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110462060A (zh) * | 2017-12-08 | 2019-11-15 | 10X基因组学有限公司 | 用于标记细胞的方法和组合物 |
Families Citing this family (95)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10400280B2 (en) | 2012-08-14 | 2019-09-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10752949B2 (en) | 2012-08-14 | 2020-08-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10221442B2 (en) | 2012-08-14 | 2019-03-05 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US9701998B2 (en) | 2012-12-14 | 2017-07-11 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10273541B2 (en) | 2012-08-14 | 2019-04-30 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US9951386B2 (en) | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US11591637B2 (en) | 2012-08-14 | 2023-02-28 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| EP2885418A4 (en) | 2012-08-14 | 2016-03-02 | 10X Genomics Inc | MICROCAPSE COMPOSITIONS AND METHOD THEREFOR |
| WO2014093676A1 (en) | 2012-12-14 | 2014-06-19 | 10X Technologies, Inc. | Methods and systems for processing polynucleotides |
| US10533221B2 (en) | 2012-12-14 | 2020-01-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| KR20230003659A (ko) | 2013-02-08 | 2023-01-06 | 10엑스 제노믹스, 인크. | 폴리뉴클레오티드 바코드 생성 |
| US10395758B2 (en) | 2013-08-30 | 2019-08-27 | 10X Genomics, Inc. | Sequencing methods |
| US9824068B2 (en) | 2013-12-16 | 2017-11-21 | 10X Genomics, Inc. | Methods and apparatus for sorting data |
| EP4219010A1 (en) | 2014-04-10 | 2023-08-02 | 10X Genomics, Inc. | Methods for encapsulating and partitioning reagents |
| WO2015200541A1 (en) | 2014-06-24 | 2015-12-30 | Bio-Rad Laboratories, Inc. | Digital pcr barcoding |
| WO2015200869A1 (en) | 2014-06-26 | 2015-12-30 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| US12312640B2 (en) | 2014-06-26 | 2025-05-27 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| CN106575322B (zh) | 2014-06-26 | 2019-06-18 | 10X基因组学有限公司 | 核酸序列装配的方法和系统 |
| MX2016016902A (es) | 2014-06-26 | 2017-03-27 | 10X Genomics Inc | Metodos para analizar acidos nucleicos de celulas individuales o poblaciones de celulas. |
| JP2017532042A (ja) | 2014-10-29 | 2017-11-02 | １０エックス ゲノミクス，インコーポレイテッド | 標的化核酸配列決定のための方法及び組成物 |
| US9975122B2 (en) | 2014-11-05 | 2018-05-22 | 10X Genomics, Inc. | Instrument systems for integrated sample processing |
| EP3244992B1 (en) | 2015-01-12 | 2023-03-08 | 10X Genomics, Inc. | Processes for barcoding nucleic acids |
| CA2968417A1 (en) | 2015-01-13 | 2016-07-21 | 10X Genomics, Inc. | Systems and methods for visualizing structural variation and phasing information |
| EP3256606B1 (en) | 2015-02-09 | 2019-05-22 | 10X Genomics, Inc. | Systems and methods for determining structural variation |
| US10697000B2 (en) | 2015-02-24 | 2020-06-30 | 10X Genomics, Inc. | Partition processing methods and systems |
| CA2975958A1 (en) | 2015-02-24 | 2016-09-01 | 10X Genomics, Inc. | Methods for targeted nucleic acid sequence coverage |
| US11371094B2 (en) | 2015-11-19 | 2022-06-28 | 10X Genomics, Inc. | Systems and methods for nucleic acid processing using degenerate nucleotides |
| PT3882357T (pt) | 2015-12-04 | 2022-09-05 | 10X Genomics Inc | Métodos e composições para análise de ácidos nucleicos |
| SG11201806757XA (en) | 2016-02-11 | 2018-09-27 | 10X Genomics Inc | Systems, methods, and media for de novo assembly of whole genome sequence data |
| WO2017197343A2 (en) | 2016-05-12 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic on-chip filters |
| WO2017197338A1 (en) | 2016-05-13 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic systems and methods of use |
| US10550429B2 (en) | 2016-12-22 | 2020-02-04 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10815525B2 (en) | 2016-12-22 | 2020-10-27 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10011872B1 (en) | 2016-12-22 | 2018-07-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| CN110214186B (zh) | 2017-01-30 | 2023-11-24 | 10X基因组学有限公司 | 用于基于微滴的单细胞条形编码的方法和系统 |
| US12264411B2 (en) | 2017-01-30 | 2025-04-01 | 10X Genomics, Inc. | Methods and systems for analysis |
| US10995333B2 (en) | 2017-02-06 | 2021-05-04 | 10X Genomics, Inc. | Systems and methods for nucleic acid preparation |
| SG11201908596WA (en) | 2017-04-26 | 2019-11-28 | 10X Genomics Inc | Mmlv reverse transcriptase variants |
| EP4215616B1 (en) | 2017-05-18 | 2024-09-04 | 10X Genomics, Inc. | Methods and systems for sorting droplets and beads |
| US10544413B2 (en) | 2017-05-18 | 2020-01-28 | 10X Genomics, Inc. | Methods and systems for sorting droplets and beads |
| WO2018213774A1 (en) | 2017-05-19 | 2018-11-22 | 10X Genomics, Inc. | Systems and methods for analyzing datasets |
| US10844372B2 (en) | 2017-05-26 | 2020-11-24 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
| CN116064732A (zh) | 2017-05-26 | 2023-05-05 | 10X基因组学有限公司 | 转座酶可接近性染色质的单细胞分析 |
| KR101979834B1 (ko) * | 2017-08-11 | 2019-05-17 | 한국과학기술원 | 미세유체장치를 이용한 다중유전자 디지털 신호 분석장치 및 이의 분석방법 |
| US10610865B2 (en) | 2017-08-22 | 2020-04-07 | 10X Genomics, Inc. | Droplet forming devices and system with differential surface properties |
| US10748643B2 (en) | 2017-08-31 | 2020-08-18 | 10X Genomics, Inc. | Systems and methods for determining the integrity of test strings with respect to a ground truth string |
| US10590244B2 (en) | 2017-10-04 | 2020-03-17 | 10X Genomics, Inc. | Compositions, methods, and systems for bead formation using improved polymers |
| US10837047B2 (en) | 2017-10-04 | 2020-11-17 | 10X Genomics, Inc. | Compositions, methods, and systems for bead formation using improved polymers |
| WO2019084043A1 (en) | 2017-10-26 | 2019-05-02 | 10X Genomics, Inc. | METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS |
| WO2019083852A1 (en) | 2017-10-26 | 2019-05-02 | 10X Genomics, Inc. | MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING |
| CN114525273B (zh) | 2017-10-27 | 2025-01-28 | 10X基因组学有限公司 | 用于样品制备和分析的方法和系统 |
| WO2019099751A1 (en) | 2017-11-15 | 2019-05-23 | 10X Genomics, Inc. | Functionalized gel beads |
| US10829815B2 (en) | 2017-11-17 | 2020-11-10 | 10X Genomics, Inc. | Methods and systems for associating physical and genetic properties of biological particles |
| WO2019108851A1 (en) | 2017-11-30 | 2019-06-06 | 10X Genomics, Inc. | Systems and methods for nucleic acid preparation and analysis |
| CN118818037A (zh) | 2017-12-12 | 2024-10-22 | 10X基因组学有限公司 | 用于单细胞处理的系统和方法 |
| CN111712579B (zh) | 2017-12-22 | 2024-10-15 | 10X基因组学有限公司 | 用于处理来自一个或多个细胞的核酸分子的系统和方法 |
| WO2019157529A1 (en) | 2018-02-12 | 2019-08-15 | 10X Genomics, Inc. | Methods characterizing multiple analytes from individual cells or cell populations |
| US11639928B2 (en) | 2018-02-22 | 2023-05-02 | 10X Genomics, Inc. | Methods and systems for characterizing analytes from individual cells or cell populations |
| WO2019169028A1 (en) | 2018-02-28 | 2019-09-06 | 10X Genomics, Inc. | Transcriptome sequencing through random ligation |
| WO2019195166A1 (en) | 2018-04-06 | 2019-10-10 | 10X Genomics, Inc. | Systems and methods for quality control in single cell processing |
| KR101913735B1 (ko) * | 2018-05-03 | 2018-11-01 | 주식회사 셀레믹스 | 차세대 염기서열 분석을 위한 시료 간 교차 오염 탐색용 내부 검정 물질 |
| WO2019217758A1 (en) | 2018-05-10 | 2019-11-14 | 10X Genomics, Inc. | Methods and systems for molecular library generation |
| US11932899B2 (en) | 2018-06-07 | 2024-03-19 | 10X Genomics, Inc. | Methods and systems for characterizing nucleic acid molecules |
| US11703427B2 (en) | 2018-06-25 | 2023-07-18 | 10X Genomics, Inc. | Methods and systems for cell and bead processing |
| US12188014B1 (en) | 2018-07-25 | 2025-01-07 | 10X Genomics, Inc. | Compositions and methods for nucleic acid processing using blocking agents |
| US20200032335A1 (en) | 2018-07-27 | 2020-01-30 | 10X Genomics, Inc. | Systems and methods for metabolome analysis |
| WO2020028882A1 (en) | 2018-08-03 | 2020-02-06 | 10X Genomics, Inc. | Methods and systems to minimize barcode exchange |
| US12065688B2 (en) | 2018-08-20 | 2024-08-20 | 10X Genomics, Inc. | Compositions and methods for cellular processing |
| WO2020041148A1 (en) | 2018-08-20 | 2020-02-27 | 10X Genomics, Inc. | Methods and systems for detection of protein-dna interactions using proximity ligation |
| US11459607B1 (en) | 2018-12-10 | 2022-10-04 | 10X Genomics, Inc. | Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes |
| TWI725686B (zh) | 2018-12-26 | 2021-04-21 | 財團法人工業技術研究院 | 用於產生液珠的管狀結構及液珠產生方法 |
| US12169198B2 (en) | 2019-01-08 | 2024-12-17 | 10X Genomics, Inc. | Systems and methods for sample analysis |
| US11845983B1 (en) | 2019-01-09 | 2023-12-19 | 10X Genomics, Inc. | Methods and systems for multiplexing of droplet based assays |
| WO2020167862A1 (en) | 2019-02-12 | 2020-08-20 | 10X Genomics, Inc. | Systems and methods for transfer of reagents between droplets |
| WO2020167866A1 (en) | 2019-02-12 | 2020-08-20 | 10X Genomics, Inc. | Systems and methods for transposon loading |
| US11851683B1 (en) | 2019-02-12 | 2023-12-26 | 10X Genomics, Inc. | Methods and systems for selective analysis of cellular samples |
| US12305239B2 (en) | 2019-02-12 | 2025-05-20 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| US11584953B2 (en) | 2019-02-12 | 2023-02-21 | 10X Genomics, Inc. | Methods for processing nucleic acid molecules |
| US12275993B2 (en) | 2019-02-12 | 2025-04-15 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| US11467153B2 (en) | 2019-02-12 | 2022-10-11 | 10X Genomics, Inc. | Methods for processing nucleic acid molecules |
| US11655499B1 (en) | 2019-02-25 | 2023-05-23 | 10X Genomics, Inc. | Detection of sequence elements in nucleic acid molecules |
| WO2020185791A1 (en) | 2019-03-11 | 2020-09-17 | 10X Genomics, Inc. | Systems and methods for processing optically tagged beads |
| US12235262B1 (en) | 2019-09-09 | 2025-02-25 | 10X Genomics, Inc. | Methods and systems for single cell protein analysis |
| EP4073241A2 (en) | 2019-12-11 | 2022-10-19 | 10X Genomics, Inc. | Reverse transcriptase variants |
| US12112833B2 (en) | 2020-02-04 | 2024-10-08 | 10X Genomics, Inc. | Systems and methods for index hopping filtering |
| US12421558B2 (en) | 2020-02-13 | 2025-09-23 | 10X Genomics, Inc. | Systems and methods for joint interactive visualization of gene expression and DNA chromatin accessibility |
| US11851700B1 (en) | 2020-05-13 | 2023-12-26 | 10X Genomics, Inc. | Methods, kits, and compositions for processing extracellular molecules |
| US12168801B1 (en) | 2020-07-02 | 2024-12-17 | 10X Genomics, Inc. | Hybrid/capture probe designs for full-length cDNA |
| US12480158B1 (en) | 2020-11-05 | 2025-11-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US12084715B1 (en) | 2020-11-05 | 2024-09-10 | 10X Genomics, Inc. | Methods and systems for reducing artifactual antisense products |
| US12398262B1 (en) | 2021-01-22 | 2025-08-26 | 10X Genomics, Inc. | Triblock copolymer-based cell stabilization and fixation system and methods of use thereof |
| WO2022182682A1 (en) | 2021-02-23 | 2022-09-01 | 10X Genomics, Inc. | Probe-based analysis of nucleic acids and proteins |
| WO2024006392A1 (en) * | 2022-06-29 | 2024-01-04 | 10X Genomics, Inc. | Probe-based analysis of nucleic acids and proteins |
| US12467087B1 (en) | 2024-06-25 | 2025-11-11 | Guardant Health, Inc. | Sequencing methods with partitioning |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012047889A2 (en) * | 2010-10-04 | 2012-04-12 | Genapsys Inc. | Systems and methods for automated reusable parallel biological reactions |
| WO2012112970A2 (en) * | 2011-02-18 | 2012-08-23 | Bio-Rad Laboratories, Inc. | Methods and compositions for detecting genetic material |
| US20120316074A1 (en) * | 2011-04-25 | 2012-12-13 | Bio-Rad Laboratories, Inc. | Methods and compositions for nucleic acid analysis |
| WO2013019751A1 (en) * | 2011-07-29 | 2013-02-07 | Bio-Rad Laboratories, Inc., | Library characterization by digital assay |
| US20130130919A1 (en) * | 2011-10-18 | 2013-05-23 | The Regents Of The University Of California | Long-Range Barcode Labeling-Sequencing |
| US20130225418A1 (en) * | 2012-02-24 | 2013-08-29 | Andrew Watson | Labeling and sample preparation for sequencing |
| WO2014028537A1 (en) * | 2012-08-14 | 2014-02-20 | 10X Technologies, Inc. | Microcapsule compositions and methods |
| WO2014074611A1 (en) * | 2012-11-07 | 2014-05-15 | Good Start Genetics, Inc. | Methods and systems for identifying contamination in samples |
| WO2014093676A1 (en) * | 2012-12-14 | 2014-06-19 | 10X Technologies, Inc. | Methods and systems for processing polynucleotides |
| WO2014210353A2 (en) * | 2013-06-27 | 2014-12-31 | 10X Technologies, Inc. | Compositions and methods for sample processing |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2385143B1 (en) * | 2006-02-02 | 2016-07-13 | The Board of Trustees of the Leland Stanford Junior University | Non-invasive fetal genetic screening by digital analysis |
| WO2008134153A1 (en) * | 2007-04-23 | 2008-11-06 | Advanced Liquid Logic, Inc. | Bead-based multiplexed analytical methods and instrumentation |
| US9625454B2 (en) * | 2009-09-04 | 2017-04-18 | The Research Foundation For The State University Of New York | Rapid and continuous analyte processing in droplet microfluidic devices |
| WO2012112804A1 (en) * | 2011-02-18 | 2012-08-23 | Raindance Technoligies, Inc. | Compositions and methods for molecular labeling |
-
2015
- 2015-06-26 EP EP15812045.1A patent/EP3161161A4/en not_active Withdrawn
- 2015-06-26 CA CA2953473A patent/CA2953473A1/en not_active Abandoned
- 2015-06-26 AU AU2015279619A patent/AU2015279619A1/en not_active Abandoned
- 2015-06-26 US US14/752,602 patent/US20150376605A1/en not_active Abandoned
- 2015-06-26 KR KR1020167036360A patent/KR20170023011A/ko not_active Withdrawn
- 2015-06-26 CN CN201580034146.0A patent/CN106574298A/zh active Pending
- 2015-06-26 MX MX2016016898A patent/MX2016016898A/es unknown
- 2015-06-26 JP JP2016575081A patent/JP2017523774A/ja active Pending
- 2015-06-26 WO PCT/US2015/038143 patent/WO2015200871A1/en not_active Ceased
-
2016
- 2016-12-18 IL IL249618A patent/IL249618A0/en unknown
-
2019
- 2019-12-23 US US16/725,673 patent/US20200399631A1/en not_active Abandoned
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012047889A2 (en) * | 2010-10-04 | 2012-04-12 | Genapsys Inc. | Systems and methods for automated reusable parallel biological reactions |
| WO2012112970A2 (en) * | 2011-02-18 | 2012-08-23 | Bio-Rad Laboratories, Inc. | Methods and compositions for detecting genetic material |
| US20120316074A1 (en) * | 2011-04-25 | 2012-12-13 | Bio-Rad Laboratories, Inc. | Methods and compositions for nucleic acid analysis |
| WO2013019751A1 (en) * | 2011-07-29 | 2013-02-07 | Bio-Rad Laboratories, Inc., | Library characterization by digital assay |
| US20130130919A1 (en) * | 2011-10-18 | 2013-05-23 | The Regents Of The University Of California | Long-Range Barcode Labeling-Sequencing |
| US20130225418A1 (en) * | 2012-02-24 | 2013-08-29 | Andrew Watson | Labeling and sample preparation for sequencing |
| WO2014028537A1 (en) * | 2012-08-14 | 2014-02-20 | 10X Technologies, Inc. | Microcapsule compositions and methods |
| WO2014074611A1 (en) * | 2012-11-07 | 2014-05-15 | Good Start Genetics, Inc. | Methods and systems for identifying contamination in samples |
| WO2014093676A1 (en) * | 2012-12-14 | 2014-06-19 | 10X Technologies, Inc. | Methods and systems for processing polynucleotides |
| WO2014210353A2 (en) * | 2013-06-27 | 2014-12-31 | 10X Technologies, Inc. | Compositions and methods for sample processing |
Non-Patent Citations (1)
| Title |
|---|
| ROBERT SCHMIEDER等: "Fast Identification and Removal of Sequence Contamination from Genomic and Metagenomic Datasets", 《PLOS ONE》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110462060A (zh) * | 2017-12-08 | 2019-11-15 | 10X基因组学有限公司 | 用于标记细胞的方法和组合物 |
| CN110462060B (zh) * | 2017-12-08 | 2022-05-03 | 10X基因组学有限公司 | 用于标记细胞的方法和组合物 |
Also Published As
| Publication number | Publication date |
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| US20200399631A1 (en) | 2020-12-24 |
| JP2017523774A (ja) | 2017-08-24 |
| AU2015279619A1 (en) | 2017-01-12 |
| US20150376605A1 (en) | 2015-12-31 |
| CA2953473A1 (en) | 2015-12-30 |
| KR20170023011A (ko) | 2017-03-02 |
| WO2015200871A1 (en) | 2015-12-30 |
| EP3161161A4 (en) | 2018-02-28 |
| EP3161161A1 (en) | 2017-05-03 |
| MX2016016898A (es) | 2017-04-25 |
| IL249618A0 (en) | 2017-02-28 |
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