JP2017523774A - サンプル分析のための方法及び組成物 - Google Patents
サンプル分析のための方法及び組成物 Download PDFInfo
- Publication number
- JP2017523774A JP2017523774A JP2016575081A JP2016575081A JP2017523774A JP 2017523774 A JP2017523774 A JP 2017523774A JP 2016575081 A JP2016575081 A JP 2016575081A JP 2016575081 A JP2016575081 A JP 2016575081A JP 2017523774 A JP2017523774 A JP 2017523774A
- Authority
- JP
- Japan
- Prior art keywords
- read data
- nucleic acid
- sequence
- less
- contaminant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
- C12Q2535/122—Massive parallel sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/159—Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Ecology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462017580P | 2014-06-26 | 2014-06-26 | |
| US62/017,580 | 2014-06-26 | ||
| US201462063870P | 2014-10-14 | 2014-10-14 | |
| US62/063,870 | 2014-10-14 | ||
| PCT/US2015/038143 WO2015200871A1 (en) | 2014-06-26 | 2015-06-26 | Methods and compositions for sample analysis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2017523774A true JP2017523774A (ja) | 2017-08-24 |
| JP2017523774A5 JP2017523774A5 (enExample) | 2018-08-02 |
Family
ID=54929861
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016575081A Pending JP2017523774A (ja) | 2014-06-26 | 2015-06-26 | サンプル分析のための方法及び組成物 |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US20150376605A1 (enExample) |
| EP (1) | EP3161161A4 (enExample) |
| JP (1) | JP2017523774A (enExample) |
| KR (1) | KR20170023011A (enExample) |
| CN (1) | CN106574298A (enExample) |
| AU (1) | AU2015279619A1 (enExample) |
| CA (1) | CA2953473A1 (enExample) |
| IL (1) | IL249618A0 (enExample) |
| MX (1) | MX2016016898A (enExample) |
| WO (1) | WO2015200871A1 (enExample) |
Families Citing this family (96)
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| US11591637B2 (en) | 2012-08-14 | 2023-02-28 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US10221442B2 (en) | 2012-08-14 | 2019-03-05 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US10752949B2 (en) | 2012-08-14 | 2020-08-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10273541B2 (en) | 2012-08-14 | 2019-04-30 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US9701998B2 (en) | 2012-12-14 | 2017-07-11 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| EP2885418A4 (en) | 2012-08-14 | 2016-03-02 | 10X Genomics Inc | MICROCAPSE COMPOSITIONS AND METHOD THEREFOR |
| US9951386B2 (en) | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10584381B2 (en) | 2012-08-14 | 2020-03-10 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| WO2014093676A1 (en) | 2012-12-14 | 2014-06-19 | 10X Technologies, Inc. | Methods and systems for processing polynucleotides |
| US10533221B2 (en) | 2012-12-14 | 2020-01-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| CN108753766A (zh) | 2013-02-08 | 2018-11-06 | 10X基因组学有限公司 | 多核苷酸条形码生成 |
| US10395758B2 (en) | 2013-08-30 | 2019-08-27 | 10X Genomics, Inc. | Sequencing methods |
| US9824068B2 (en) | 2013-12-16 | 2017-11-21 | 10X Genomics, Inc. | Methods and apparatus for sorting data |
| KR102596508B1 (ko) | 2014-04-10 | 2023-10-30 | 10엑스 제노믹스, 인크. | 시약을 캡슐화 및 구획화하기 위한 유체 디바이스, 시스템, 및 방법, 및 이의 응용 |
| CN114214314A (zh) | 2014-06-24 | 2022-03-22 | 生物辐射实验室股份有限公司 | 数字式pcr条码化 |
| EP3161700B1 (en) | 2014-06-26 | 2023-03-29 | 10X Genomics, Inc. | Processes and systems for nucleic acid sequence assembly |
| CA2953469A1 (en) | 2014-06-26 | 2015-12-30 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| US12312640B2 (en) | 2014-06-26 | 2025-05-27 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| EP4574974A3 (en) | 2014-06-26 | 2025-10-08 | 10x Genomics, Inc. | Methods of analyzing nucleic acids from individual cells or cell populations |
| KR20170073667A (ko) | 2014-10-29 | 2017-06-28 | 10엑스 제노믹스, 인크. | 표적화 핵산 서열 분석을 위한 방법 및 조성물 |
| US9975122B2 (en) | 2014-11-05 | 2018-05-22 | 10X Genomics, Inc. | Instrument systems for integrated sample processing |
| US10221436B2 (en) | 2015-01-12 | 2019-03-05 | 10X Genomics, Inc. | Processes and systems for preparation of nucleic acid sequencing libraries and libraries prepared using same |
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| CN107208156B (zh) | 2015-02-09 | 2021-10-08 | 10X基因组学有限公司 | 用于使用变异识别数据来确定结构变异和定相的系统和方法 |
| MX2017010857A (es) | 2015-02-24 | 2017-12-11 | 10X Genomics Inc | Metodos para la cobertura de secuencia de acidos nucleicos seleccionados como diana. |
| WO2016137973A1 (en) | 2015-02-24 | 2016-09-01 | 10X Genomics Inc | Partition processing methods and systems |
| US11371094B2 (en) | 2015-11-19 | 2022-06-28 | 10X Genomics, Inc. | Systems and methods for nucleic acid processing using degenerate nucleotides |
| SG10202108763UA (en) | 2015-12-04 | 2021-09-29 | 10X Genomics Inc | Methods and compositions for nucleic acid analysis |
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| US10550429B2 (en) | 2016-12-22 | 2020-02-04 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10011872B1 (en) | 2016-12-22 | 2018-07-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10815525B2 (en) | 2016-12-22 | 2020-10-27 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
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| US11655499B1 (en) | 2019-02-25 | 2023-05-23 | 10X Genomics, Inc. | Detection of sequence elements in nucleic acid molecules |
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| US12235262B1 (en) | 2019-09-09 | 2025-02-25 | 10X Genomics, Inc. | Methods and systems for single cell protein analysis |
| EP4461810A3 (en) | 2019-12-11 | 2025-04-09 | 10X Genomics, Inc. | Reverse transcriptase variants |
| US12112833B2 (en) | 2020-02-04 | 2024-10-08 | 10X Genomics, Inc. | Systems and methods for index hopping filtering |
| CN115428088A (zh) | 2020-02-13 | 2022-12-02 | 10X基因组学有限公司 | 用于基因表达和dna染色质可及性的联合交互式可视化的系统和方法 |
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| US12168801B1 (en) | 2020-07-02 | 2024-12-17 | 10X Genomics, Inc. | Hybrid/capture probe designs for full-length cDNA |
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| WO2014028537A1 (en) * | 2012-08-14 | 2014-02-20 | 10X Technologies, Inc. | Microcapsule compositions and methods |
| WO2014093676A1 (en) * | 2012-12-14 | 2014-06-19 | 10X Technologies, Inc. | Methods and systems for processing polynucleotides |
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| AU2014302277A1 (en) * | 2013-06-27 | 2015-12-24 | 10X Genomics, Inc. | Compositions and methods for sample processing |
-
2015
- 2015-06-26 US US14/752,602 patent/US20150376605A1/en not_active Abandoned
- 2015-06-26 KR KR1020167036360A patent/KR20170023011A/ko not_active Withdrawn
- 2015-06-26 AU AU2015279619A patent/AU2015279619A1/en not_active Abandoned
- 2015-06-26 CN CN201580034146.0A patent/CN106574298A/zh active Pending
- 2015-06-26 MX MX2016016898A patent/MX2016016898A/es unknown
- 2015-06-26 WO PCT/US2015/038143 patent/WO2015200871A1/en not_active Ceased
- 2015-06-26 JP JP2016575081A patent/JP2017523774A/ja active Pending
- 2015-06-26 EP EP15812045.1A patent/EP3161161A4/en not_active Withdrawn
- 2015-06-26 CA CA2953473A patent/CA2953473A1/en not_active Abandoned
-
2016
- 2016-12-18 IL IL249618A patent/IL249618A0/en unknown
-
2019
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014028537A1 (en) * | 2012-08-14 | 2014-02-20 | 10X Technologies, Inc. | Microcapsule compositions and methods |
| WO2014093676A1 (en) * | 2012-12-14 | 2014-06-19 | 10X Technologies, Inc. | Methods and systems for processing polynucleotides |
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|---|---|
| IL249618A0 (en) | 2017-02-28 |
| MX2016016898A (es) | 2017-04-25 |
| US20200399631A1 (en) | 2020-12-24 |
| CA2953473A1 (en) | 2015-12-30 |
| WO2015200871A1 (en) | 2015-12-30 |
| US20150376605A1 (en) | 2015-12-31 |
| EP3161161A1 (en) | 2017-05-03 |
| EP3161161A4 (en) | 2018-02-28 |
| CN106574298A (zh) | 2017-04-19 |
| KR20170023011A (ko) | 2017-03-02 |
| AU2015279619A1 (en) | 2017-01-12 |
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