CN106550939A - A kind of method that Radix seu cortex hberberidis heteropodae seed is preserved for a long time - Google Patents
A kind of method that Radix seu cortex hberberidis heteropodae seed is preserved for a long time Download PDFInfo
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- CN106550939A CN106550939A CN201610922686.9A CN201610922686A CN106550939A CN 106550939 A CN106550939 A CN 106550939A CN 201610922686 A CN201610922686 A CN 201610922686A CN 106550939 A CN106550939 A CN 106550939A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
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- Agronomy & Crop Science (AREA)
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Abstract
The invention discloses a kind of method that Radix seu cortex hberberidis heteropodae seed is preserved for a long time, comprises the steps：Dry in the sun will be carried out after seed sterilizing, then dried Radix seu cortex hberberidis heteropodae seed is placed under the conditions of 2～5 DEG C, soaked 45～60 minutes in the mixed liquor of ethylene glycol 15%+ dimethyl sulfoxide (DMSO) (10%)+sucrose 10%；The Radix seu cortex hberberidis heteropodae seed that precooling is handled well is placed in absorbent carbasuss bag, is placed in the exsiccator that lower floor is silica gel together with gauze and is dried；Then ceresine is equably smeared on seed after the drying, after the completion of smearing, by seed tinfoil paper paper bag airtight package, first being put under the low temperature environment that temperature is 25 DEG C～40 DEG C and preserve 30～60 minutes, then be loaded into the hermetic bag filled with liquid nitrogen (196 degrees Celsius) carries out Excised Embryos.The present invention can keep seed activity for a long time, it is ensured that percentage of seedgermination, improve emergence rate and seedling establishment ability.
Description
Technical field
The present invention relates to Seed storage field, and in particular to a kind of method that Radix seu cortex hberberidis heteropodae seed is preserved for a long time.
Background technology
Radix seu cortex hberberidis heteropodae (scientific name：Berberis atrocarpa) be Berberidaceae Berberiss plant, be China peculiar plant
Thing.The ground such as Hunan of China's Mainland, Yunnan, Sichuan, Xinjiang are distributed in, the area of 600 meters to 2,800 meters of height above sea level is grown on.It is black
Fruit Radix Berberidis Amurensis are the dominant trees of the shallow mountain belts distribution of arid biogeographic zone, have important ecological work(at aspects such as water conservation, soil conservations
Energy.Meanwhile, Radix seu cortex hberberidis heteropodae is good wild plant medicine, has been widely used and payes attention in terms of national medicine, and Radix osteomelis schwerinais are little
The peel of stem and root of bark of a cork tree all contains berberine, pathogenic fire purging of can detoxifying of being used as medicine, fruit medicine, can stomach invigorating, heat-clearing and toxic substances removing.For gastroenteritis,
Dysentery, dyspepsia and vitamin C deficiency etc..Because which is widely distributed, and there is above-mentioned medicinal health care function, with larger
Developing and utilizingpotentiality.
At present due to being disturbed by mankind's high intensity activity, Radix seu cortex hberberidis heteropodae group constantly degenerates, and year has lower productivity only, resists
Interference performance and stability weaken, it is natural more can ability it is weak.One of the reason for restriction arid biogeographic zone Radix seu cortex hberberidis heteropodae population recruitment is to plant
The sub- holding time is short, and germinating capacity is weak.The life-span of Radix seu cortex hberberidis heteropodae seed is 25～40 days under field conditions (factors).In traditional renewal
During, after fruit maturation, seed is deposited at normal temperatures, such as sowed not in time, then largely reduce sending out for seed
Bud ability, limits natural regeneration.
Modern Seed is preserved, and actually adopts refrigerating and air conditioning technology in time, along with control seed moisture content and seed packaging
Closing, so as to reach low temperature, low humidity, closed preservation condition, makes seed safety, long-term preservation.High damp condition is promoted
The growth of mycete so that seed mildew or insect pest occur.High temperature accelerates enzymatic reaction.Therefore, control suitable temperature and wet
Degree is the essential condition for preserving seed for a long time.But typically should the control condition of low temperature low humidity again be not readily reachable by.Solve at present
Certainly low humidity mainly has by desiccant to control water content；Seed is mainly placed in -196 DEG C of liquid nitrogen and is preserved by low humidity.
But if directly seed not being put in liquid nitrogen, the cellularity of seed can be destroyed, while seed is by cryoprotection process
Make also got damp after ingress of air after drying again in desiccant, do not utilize the preservation of seed.
To sum up, it is badly in need of a kind of method that Radix seu cortex hberberidis heteropodae seed is preserved for a long time in Radix seu cortex hberberidis heteropodae renewal process, to protect for a long time
Hold seed activity, it is ensured that percentage of seedgermination, improve emergence rate and seedling establishment ability.
The content of the invention
To solve the above problems, the invention provides a kind of method that Radix seu cortex hberberidis heteropodae seed is preserved for a long time, can be protected for a long time
Hold seed activity, it is ensured that percentage of seedgermination, improve emergence rate and seedling establishment ability.
For achieving the above object, the technical scheme taken of the present invention is：
A kind of method that Radix seu cortex hberberidis heteropodae seed is preserved for a long time, comprises the steps：
S1, sterilizing：After health full Radix seu cortex hberberidis heteropodae seed is soaked 45～60 minutes in carbendazim soak,
With distilled water flushing 3～5 times；Then seed is laid on the mesh screen with yoke, the thickness of tiling is 1～1.5cm；In sulfur
Sulfur powder is added in fumigating device, temperature be under conditions of 30 DEG C to mesh screen on the seed that tiles carry out stifling 1～1.5h；Often
Fumigate once within 5～7 days, continuous fumigation 2～3 times；
S2, seed dry in the sun：Radix seu cortex hberberidis heteropodae seed after sterilizing is placed on into dry in the sun 3～5 days on seed dry in the sun box, often
1～2h is stirred once；Forbid seed directly contact soil or concrete floor dry in the sun directly, the seed for otherwise contacting ground can be because
High temperature and affect intraseminal enzymatic activity；
S3, the precooling of cryoprotection process：Dried Radix seu cortex hberberidis heteropodae seed is placed under the conditions of -2～5 DEG C, in second
Soak 45～60 minutes in the mixed liquor of glycol I5%+ dimethyl sulfoxide (DMSO) (10%)+sucrose 10%；
S4, drying：The Radix seu cortex hberberidis heteropodae seed that precooling is handled well is placed in absorbent carbasuss bag, is placed on lower floor together with gauze
For, in the exsiccator of silica gel, silica gel is 8: 1～10: 1 with seed quality ratio, dry silica gel is changed daily, be dried 5～10 days, will plant
Sub- water content control is between 2.9～6.9%；
S5, ceresine protection seed get damp once again：In order to prevent dried seed from made moist after taking out once again, sponge is stained with
Ceresine, equably smears 3～4 times on seed, then falls ceresine brush with absorbent carbasuss；
S6, deepfreeze：Seed after ceresine is smeared tinfoil paper paper bag airtight package, be first put into temperature for -25 DEG C～
Under 40 DEG C of low temperature environment preserve 30～60 minutes, then be loaded into the hermetic bag filled with liquid nitrogen (- 196 degrees Celsius) carry out it is ultralow
Temperature is preserved.
Preferably, in step S1, carbendazim soak is formed with water exchange by 50% carbendazol wettable powder,
2～2.5L is watered per the 50% of 8～10 grams carbendazol wettable powder.
Preferably, the seed dry in the sun box surrounding in step S2 is wide 2-4cm, the batten of high 3-5cm, and length can be according to need
Make by oneself, the bottom of dry in the sun box for good permeability aperture be 8 mesh high density polyethylene (HDPE) (HDPE) fly net.
The invention has the advantages that：
1st, seed is protected by ceresine, is realized moistureproof once again：Seed moisture content is the main of impact seed retention time
One of factor.During conventional seed drying, seed moisture content is controlled using desiccant simply.But actually seed
As long as with air contact, the moisture in air can enter the preservation that seed is affected in seed once again by the Repiration of seed
Time, seed longeivity, seed vitality.This patent is by smearing the ground with moisture-proof function on the seed after desiccant dryness
Wax controls seed moisture content；
2nd, precooling is carried out before deepfreeze using cryoprotective agent, effectively in restraint seed moisture crystallization
Journey, protects seed cell structure.
3rd, seed activity is kept for a long time, it is ensured that percentage of seedgermination, improve emergence rate and seedling establishment ability, reduce and avoid
Germplasm is lost in, and effectively protects germ plasm resource and biological heredity multiformity, is especially the shallow mountain belts dominant shrub species Radix osteomelis schwerinais of arid biogeographic zone
Radix Berberidis Amurensis update and recover to provide technological guidance.
Specific embodiment
In order that objects and advantages of the present invention become more apparent, with reference to embodiments the present invention is carried out further
Describe in detail.It should be appreciated that specific embodiment described herein is not used to limit this only to explain the present invention
It is bright.
Embodiment 1
A kind of method that Radix seu cortex hberberidis heteropodae seed is preserved for a long time, comprises the steps：
Disinfection：The healthy Radix seu cortex hberberidis heteropodae kind that will be gathered in board house ditch branch office of Xinjiang Tianshan east management of state-owned forest office
Son, obtains seed after taking out outer peel, is soaked 45 minutes after drying naturally in carbendazim soak.Per 8 grams 50% it is many
Bacterium spirit wettable powder is watered 2L.Seed is laid on the mesh screen with yoke, the thickness of tiling is 1cm.In sulfurator
Middle addition sulfur powder, carries out stifling 1h to the seed that net shines.Fumigated once per 5 days, continuous fumigation 2 times.
Seed dry in the sun：Radix seu cortex hberberidis heteropodae seed after sterilizing is placed on into dry in the sun 5 days on seed dry in the sun box, is stirred per 1h
Once.Seed dry in the sun box surrounding is wide 2cm, high 3.5cm, long 2.5 meters wooden frame, and the bottom of dry in the sun box is the hole of good permeability
Footpath is high density polyethylene (HDPE) (HDPE) fly net of 8 mesh.
Cryoprotection process：Dried Radix seu cortex hberberidis heteropodae seed is placed under the conditions of 2.5 DEG C, in ethylene glycol I5%+ diformazans
Soak 45 minutes in the mixed liquor of base sulfoxide (DMSO) (10%)+sucrose 10%.
It is dried：Dry in the sun good Radix seu cortex hberberidis heteropodae seed is placed in absorbent carbasuss bag, lower floor is placed on for silica gel together with gauze
In exsiccator.Silica gel is 10: 1 with seed quality ratio, changes dry silica gel daily.It is dried 7 days, seed moisture content is controlled 5.5
Between～6.0%.
Ceresine protects seed：In order to prevent dried seed from made moist after taking out once again, sponge is stained with into ceresine, equably
Smear on seed 3 times, then ceresine brush is fallen with absorbent carbasuss.
Deepfreeze：Seed after ceresine is protected tinfoil paper paper bag airtight package, is first put into the low temperature that temperature is -30 DEG C
Preserving 45 minutes under environment, then be loaded into the hermetic bag filled with liquid nitrogen (- 196 degrees Celsius) carries out Excised Embryos.
Embodiment 2
A kind of method that Radix seu cortex hberberidis heteropodae seed is preserved for a long time, comprises the steps：
Disinfection：The healthy Radix seu cortex hberberidis heteropodae kind that will be gathered in Altai in Xinjiang Province management of state-owned forest Ju little Donggous branch office
Son, obtains seed after taking out outer peel, is soaked 60 minutes after drying naturally in carbendazim soak.Per 10 grams 50% it is many
Bacterium spirit wettable powder is watered 2.5L.Seed is laid on the mesh screen with yoke, the thickness of tiling is 1.5cm.It is smoked in sulfur
Sulfur powder is added in steaming device, stifling 1h is carried out to the seed that net shines.Fumigated once per 7 days, continuous fumigation 2 times.
Seed dry in the sun：Radix seu cortex hberberidis heteropodae seed after sterilizing is placed on into dry in the sun 5 days on seed dry in the sun box, is stirred per 1h
Once.Seed dry in the sun box surrounding is wide 2cm, high 3.5cm, long 2.5 meters wooden frame, and the bottom of dry in the sun box is the hole of good permeability
Footpath is high density polyethylene (HDPE) (HDPE) fly net of 8 mesh.
Cryoprotection process：Radix seu cortex hberberidis heteropodae seed after dry in the sun is placed under the conditions of 5 DEG C, in ethylene glycol I5%+ dimethyl
Soak 60 minutes in the mixed liquor of sulfoxide (DMSO) (10%)+sucrose 10%.
It is dried：Dry in the sun good Radix seu cortex hberberidis heteropodae seed is placed in absorbent carbasuss bag, lower floor is placed on for silica gel together with gauze
In exsiccator.Silica gel is 8: 1 with seed quality ratio, changes dry silica gel daily.It is dried 5 days, seed moisture content is controlled 2.9%
Between.
Ceresine protects seed：In order to prevent dried seed from made moist after taking out once again, sponge is stained with into ceresine, equably
Smear on seed 3 times, then ceresine brush is fallen with absorbent carbasuss.
Deepfreeze：Seed after ceresine is protected tinfoil paper paper bag airtight package, is first put into the low temperature that temperature is -30 DEG C
Preserving 30 minutes under environment, then be loaded into the hermetic bag filled with liquid nitrogen (- 196 degrees Celsius) carries out Excised Embryos.
At 1 20 DEG C of table, ceresine is smeared and is not had the Radix seu cortex hberberidis heteropodae seed moisture content that ceresine smears and compares that (initial moisture content is
6.9%)
| Holding time (my god) | Ceresine smears seed moisture content (%) | Seed moisture content (%) is smeared without ceresine |
| 15 | 6.9 | 6.9 |
| 30 | 6.9 | 7 |
| 45 | 6.8 | 7.2 |
| 60 | 6.8 | 7.2 |
| 75 | 6.7 | 7.3 |
| 90 | 6.7 | 7.2 |
| 105 | 6.7 | 7.3 |
| 120 | 6.5 | 7.3 |
| 135 | 6.5 | 7.4 |
| 150 | 6.5 | 7.4 |
| 165 | 6.5 | 7.5 |
| 180 | 6.3 | 7.6 |
Different holding time Radix seu cortex hberberidis heteropodae percentages of seedgermination (25 DEG C) under 2 different moisture content of table
(DEG C) different holding time under 3 different temperatures of table (my god) Radix seu cortex hberberidis heteropodae percentage of seedgermination (water content 6.9%)
Knowable to table 1- tables 3, with not through the seed that ceresine is smeared compared with, ceresine smears seed in longer one section
It is interior to keep stable water content；Seed is (to contain less than -10 DEG C less than 6.9% (under room temperature) and temperature in water content
The water yield be 6.9%) under the conditions of, still have germinating capacity after 1 year.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (3)
1. a kind of method that Radix seu cortex hberberidis heteropodae seed is preserved for a long time, it is characterised in that comprise the steps：
S1, sterilizing：After health full Radix seu cortex hberberidis heteropodae seed is soaked 45～60 minutes in carbendazim soak, with steaming
Distilled water is rinsed 3～5 times；Then seed is laid on the mesh screen with yoke, the thickness of tiling is 1～1.5cm；In sulfur fumigation
Sulfur powder is added in device, temperature be under conditions of 30 DEG C to mesh screen on the seed that tiles carry out stifling 1～1.5h；Per 5～7
It is stifling once, continuous fumigation 2～3 times；
S2, seed dry in the sun：Radix seu cortex hberberidis heteropodae seed after sterilizing is placed on into dry in the sun 3～5 days on seed dry in the sun box, per 1～
2h is stirred once；Forbid directly by seed directly contact soil or concrete floor dry in the sun, the seed for otherwise contacting ground can be because of height
Temperature and affect intraseminal enzymatic activity；
S3, the precooling of cryoprotection process：Dried Radix seu cortex hberberidis heteropodae seed is placed under the conditions of -2～5 DEG C, in ethylene glycol
Soak 45～60 minutes in the mixed liquor of 15%+ dimethyl sulfoxide (DMSO) (10%)+sucrose 10%；
S4, drying：The Radix seu cortex hberberidis heteropodae seed that precooling is handled well is placed in absorbent carbasuss bag, is placed on lower floor for silicon together with gauze
In the exsiccator of glue, silica gel is 8: 1～10: 1 with seed quality ratio, changes dry silica gel daily, is dried 5～10 days, seed is contained
Water rate control is between 2.9～6.9%；
S5, ceresine protection seed get damp once again：Sponge is stained with into ceresine, is equably smeared 3～4 times on seed, then is used defat
Ceresine brush is fallen by gauze；
S6, deepfreeze：Seed after ceresine is smeared tinfoil paper paper bag airtight package, is first put into temperature for -25 DEG C～40 DEG C
Low temperature environment under preserve 30～60 minutes, then be loaded into the hermetic bag filled with liquid nitrogen (- 196 DEG C) and carry out Excised Embryos.
2. the method that a kind of Radix seu cortex hberberidis heteropodae seed as claimed in claim 1 is preserved for a long time, it is characterised in that in step S1
Carbendazim soak is formed with water exchange by 50% carbendazol wettable powder, and the carbendazim per the 50% of 8～10 grams is wettable
Property powder is watered 2～2.5L.
3. the method that a kind of Radix seu cortex hberberidis heteropodae seed as claimed in claim 1 is preserved for a long time, it is characterised in that in step S2
Seed dry in the sun box surrounding be wide 2-4cm, the batten of high 3-5cm, length can be made by oneself as needed, and the bottom of dry in the sun box is breathability
Good aperture is high density polyethylene (HDPE) (HDPE) fly net of 8 mesh.
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| CN201610922686.9A CN106550939A (en) | 2016-10-21 | 2016-10-21 | A kind of method that Radix seu cortex hberberidis heteropodae seed is preserved for a long time |
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|---|---|---|---|
| CN201610922686.9A CN106550939A (en) | 2016-10-21 | 2016-10-21 | A kind of method that Radix seu cortex hberberidis heteropodae seed is preserved for a long time |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111642170A (en) * | 2020-07-10 | 2020-09-11 | 四川省林业科学研究院 | Method for preserving seed of Acer catalpa |
| CN114747375A (en) * | 2022-04-16 | 2022-07-15 | 朱杰 | Hybrid seed storage method |
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| CN102696579A (en) * | 2012-06-05 | 2012-10-03 | 杭州师范大学 | Encapsulation-vitrification ultra-low temperature preservation method for dendrobium protocorm |
| CN104012523A (en) * | 2014-05-28 | 2014-09-03 | 中国林业科学研究院亚热带林业研究所 | Embedding vitrification ultralow-temperature storage method for bletilla striata seeds |
| CN104222072A (en) * | 2014-08-27 | 2014-12-24 | 中国农业科学院麻类研究所 | Vitrification ultralow-temperature preservation method of flax seeds |
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| CN104012523A (en) * | 2014-05-28 | 2014-09-03 | 中国林业科学研究院亚热带林业研究所 | Embedding vitrification ultralow-temperature storage method for bletilla striata seeds |
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| CN111642170A (en) * | 2020-07-10 | 2020-09-11 | 四川省林业科学研究院 | Method for preserving seed of Acer catalpa |
| CN114747375A (en) * | 2022-04-16 | 2022-07-15 | 朱杰 | Hybrid seed storage method |
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