CN1258444A - Ultra-low temperature breeding method - Google Patents
Ultra-low temperature breeding method Download PDFInfo
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- CN1258444A CN1258444A CN 99125607 CN99125607A CN1258444A CN 1258444 A CN1258444 A CN 1258444A CN 99125607 CN99125607 CN 99125607 CN 99125607 A CN99125607 A CN 99125607A CN 1258444 A CN1258444 A CN 1258444A
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Abstract
Ultra-low temperature treatment of seed can induce physical and chemical mutation inside seed, activate mutation enzyme, promote gene activation and form hereditary variation. Seed after ultra-low temperature treatment, after being unfrozen, is seeded and cultured and its varied plants are screened in the first or second generation plants and directionally subcultured to develop new variety. The present invention has both basic theory research value and application value in genetic engineering. Compared with traditional breeding method, the present invention has short period, and compared with other breeding methods, such as radiation breeding and space breeding, the present invention is simple and advanced. The present invention is suitable for crops, fruits, etc.
Description
The invention belongs to a kind of ultra-low temperature breeding method.
At present, method at sowing or plantation pre-treatment seed has of all kinds, utilize genetic modification, utilize hybrid vigour, the screening of utilization or other physics breedings are arranged, as radiation, methods such as space breeding, the breeding method of patent 96109697.9 high resistance to white leaf-blast of hybrid rice for example, 97107703.7 1 kinds of new breeding of hybridized rice of patent and seeding technique, the method of patent 99105409.1 usefulness screening methods production hybrid rice seeds etc. can be improved the breed, but effect is not very good, breeding cycle is long, equipment is more expensive, it is also remarkable to operate, be difficult for promoting, prospect is wide inadequately.
The purpose of this invention is to provide a kind of ultra-low temperature breeding method, handle seed through ultralow temperature, the inner physics and chemistry that takes place of seed is suddenlyd change, the activation of mutant enzyme impels the activation of gene, forms mutation genetic.Seed with low temperature treatment, combine respectively at potted plant and land for growing field crops plantation after thawing,, seek consistent result by potted plant detail record, variation plant is by breeding conventional treatment subculture directive breeding, is suitable for crops such as paddy rice, peanut, Chinese cabbage, watermelon and woods, the improvement of fruit kind especially.
The present invention is that the seed that will handle is packaged into parcel with gauze, paraffin paper etc., place-196 ℃ liquid nitrogen dip again, the dip time be about 24 hours after, the seed that takes out from liquid nitrogen is put into 20 ℃-90 ℃ warm water and is thawed, seeds treated, and sowing is cultivated, find the mutation plant, screen, or screen in the plant that the second generation of population mixture is separated, directed subculture is cultivated the quality product kind that makes new advances.
The present invention has genetic engineering fundamental research value and using value, has than traditional breeding way to shorten the cycle in application process, than other physics breedings, as radiation, that space breeding has more equipment is simple, easy to operate, technological means advanced person, easily promote, characteristics such as have a extensive future.
In the present invention, ultralow temperature is meant the low-temperature space of the artificial manufacturing beyond the earth natural temperature district, and promptly-70 ℃ to-270 ℃, it is low-temperature receiver that the present invention adopts-196 ℃ of liquid nitrogens, this low-temperature receiver adopt liquid nitrogen jar, thermos bottle for transportation, preserve and handle the container of seed.
Embodiment 1:1, be equipped with one liter of ice chest, adorn-196 ℃ of 1000CC liquid nitrogens;
2, the seed of peanut or paddy is packed and paraffin paper packs each 50 gram of every bag respectively with gauze;
3, the seed with equivalent compares test;
4, prepare 2000-3000CC, the 32-47 ℃ of warm water that thaws;
5, prepare 4-5 culture dish and make to cultivate usefulness;
6, be equipped with in-196 ℃ of bottles and transferring peanut, paddy seeds fast;
7, seed after dip 34-65 minute, is placed on peanut, paddy rapidly 32-47 ℃ of warm water again and thaws in liquid nitrogen;
8, the seed after will thawing is placed on and cultivates ware and educate bud, writes treating method and thawing time;
9, will contrast seed is placed on culture dish at the warm water dip after 15 minutes and educates bud;
10, do test data sheet.
Through the cryogenic testing result, peanut, paddy seeds can both sprout growths, and seed can long preservation under ultralow temperature dormancy breath state, change the seed genetic engineering and can cultivate new mutational variety, high and cold and tropical crop is transformed each other, create new varieties with new method, for the mankind benefit.
Embodiment 2: be equipped with the jar that-196 ℃ of 29 liters of liquid nitrogens are housed, with each 50 gram of paddy, use gauze respectively, paraffin paper is wrapped and is left in the jar, coexistence was put 102 days, after thawing, at 25 ℃ to 33 ℃, educate bud with culture dish, educated bud 10-24 hour, i.e. germinating growth successively, this be various enzymes in the seed in ultralow temperature sharply cataclysm in the hope of adapting to the reflection under the new environment long preservation, be seed can be long-term in the ultralow temperature liquid nitrogen strong evidence of preservation under the dormancy breath state, it can preservation be difficult for preservation and the seed that is on the verge of to disappear and rare famous and precious seed realistic meaning, in seed crossbreeding work, according to actual needs, extract male parent or maternal planting seed at any time, accurately calculate the flowering and the fertilization time of seed, ensure that breeding work carries out smoothly.
Embodiment 3: the water intaking rice, each two sample of Chinese cabbage seed, each sample 25 gram, in the tanks of liquid nitrogen of each sample with-196 ℃ of the quick inputs of gauze parcel, rice paddy seed soaked 36 hours, high pin Chinese cabbage seed soaked 15 hours, take out then, seed is dropped in the warm water seed soaking 11 hours fast, again 19 ℃ of-28 ℃ of room temperature sprout growths 144 hours, the sample of rudiment test, the five grain germination seeds of at every turn sampling, triplicate, make root, the bud length records is also made photo, and four samples of two kinds of crops are found out the isoenzymes situation of change of each group sample with its peroxidase isoenzymes activity of poly amic acid gel electrophoresis therapy determining from zymogram.Paddy rice, Chinese cabbage seed are after ultralow temperature is handled and thawed, and the root of its rudiment, bud are all compared the length of photograph, measure its corresponding peroxidase isoenzymes increased activity, have obviously promoted growing of crop germination phase.Crop seed can be cultivated the quality product kind that makes new advances after ultralow temperature is handled.
Claims (2)
1, a kind of ultra-low temperature breeding method, it is characterized in that: the seed that will handle is packaged into parcel with gauze, paraffin paper etc., place-196 ℃ liquid nitrogen dip again, the dip time be about 24 hours after, the seed that takes out from liquid nitrogen is put into 20 ℃ to 90 ℃ warm water fast and is thawed, seeds treated, sowing is cultivated routinely, finds the mutation plant, screens, or in the plant that the second generation of population mixture is separated, screen, directed subculture is cultivated the quality product kind that makes new advances.
2, the described ultra-low temperature breeding method of a tree name claim 1 is characterized in that: the ultralow temperature liquid-nitrogen temperature can be-70 ℃ to-270 ℃, and the dip time is 24 hours to several days, and thaw point is 20 ℃ to 90 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN99125607A CN1094037C (en) | 1999-11-23 | 1999-11-23 | Ultra-low temperature breeding method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN99125607A CN1094037C (en) | 1999-11-23 | 1999-11-23 | Ultra-low temperature breeding method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1258444A true CN1258444A (en) | 2000-07-05 |
| CN1094037C CN1094037C (en) | 2002-11-13 |
Family
ID=5284032
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN99125607A Expired - Fee Related CN1094037C (en) | 1999-11-23 | 1999-11-23 | Ultra-low temperature breeding method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1094037C (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106550939A (en) * | 2016-10-21 | 2017-04-05 | 新疆林科院森林生态研究所 | A kind of method that Radix seu cortex hberberidis heteropodae seed is preserved for a long time |
| CN107258413A (en) * | 2017-07-25 | 2017-10-20 | 贵州秧坝生态农业发展有限责任公司 | A kind of method that duck is put in rice cropping in a suitable place to breed |
| CN107360912A (en) * | 2017-07-25 | 2017-11-21 | 贵州秧坝生态农业发展有限责任公司 | A kind of paddy rice planting method |
| CN107493729A (en) * | 2017-09-16 | 2017-12-22 | 中国农业科学院生物技术研究所 | Promote the method for spire Africa podocarpus germination and seedling |
| CN110754359A (en) * | 2019-10-24 | 2020-02-07 | 海丰县新三农微生物农业有限公司 | Seed is little freezes low temperature cold chain and is led technology management system of educating |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1118650A (en) * | 1994-09-15 | 1996-03-20 | 湖南师范大学 | Storage method for pollen of crops |
| GB9600698D0 (en) * | 1996-01-13 | 1996-03-13 | British American Tobacco Co | Genetic transformation of trees |
-
1999
- 1999-11-23 CN CN99125607A patent/CN1094037C/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106550939A (en) * | 2016-10-21 | 2017-04-05 | 新疆林科院森林生态研究所 | A kind of method that Radix seu cortex hberberidis heteropodae seed is preserved for a long time |
| CN107258413A (en) * | 2017-07-25 | 2017-10-20 | 贵州秧坝生态农业发展有限责任公司 | A kind of method that duck is put in rice cropping in a suitable place to breed |
| CN107360912A (en) * | 2017-07-25 | 2017-11-21 | 贵州秧坝生态农业发展有限责任公司 | A kind of paddy rice planting method |
| CN107493729A (en) * | 2017-09-16 | 2017-12-22 | 中国农业科学院生物技术研究所 | Promote the method for spire Africa podocarpus germination and seedling |
| CN107493729B (en) * | 2017-09-16 | 2020-09-29 | 中国农业科学院生物技术研究所 | Method for promoting seed germination and seedling formation of African Pinus parviflora |
| CN110754359A (en) * | 2019-10-24 | 2020-02-07 | 海丰县新三农微生物农业有限公司 | Seed is little freezes low temperature cold chain and is led technology management system of educating |
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| Publication number | Publication date |
|---|---|
| CN1094037C (en) | 2002-11-13 |
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