CN106538219A - A kind of asexual reproduction method of Wild Legume shrub horse spine - Google Patents
A kind of asexual reproduction method of Wild Legume shrub horse spine Download PDFInfo
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- CN106538219A CN106538219A CN201611039722.3A CN201611039722A CN106538219A CN 106538219 A CN106538219 A CN 106538219A CN 201611039722 A CN201611039722 A CN 201611039722A CN 106538219 A CN106538219 A CN 106538219A
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- horse spine
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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Abstract
The invention provides a kind of vegetative method of Wushan horse spine, growth hormone of the method by certain concentration（Naphthalene acetic acid（NAA）, heteroauxing（IBA）, zeatin（Z）, root-inducing powder）1～24h of immersion, then cutting propagation, improves the survival rate and rootability of horse spine.Experimental results demonstrate, the process of NAA 100 6 is the preferable asexual reproduction method of Wushan horse spine, can effectively improve Wushan horse spine branch bar survival rate using the cottage method.
Description
Technical field
The present invention relates to a kind of vegetative method of horse spine, belongs to Wild Legume shrub breeding field.
Background technology
Horse spine also known as Indigofera pseudotinctoria, call long fringe Drymotaenium miyoshianum (Mak.) Mak., are the perennial undershrub of pulse family Magnolia, have barren-resistant, growth strong
Strong, well developed root system, deep ability of pricking are strong, have good compatibility with lawn plant, the features such as widely distributed, are widely used at a high speed
The greenbelt of both sides of highway, wetland and park.In addition to the ecological revetment for expressway slope, water and soil conservation, horse spine is also
Forage grass, have concurrently it is medicinal, view and admire, eat, the energy, the multiple use such as health care.Although horse spine is of many uses, germ plasm resource ten
Divide scarcity.Also biological characteristicses, cultivation and compound recipe horse spine Chinese patent medicine side are focused primarily upon at present both at home and abroad to the research of horse spine
Face, and shorter mention horse spine germplasm innovation and exploitation.It is solution to the wild collection of horse spine resource and the fast-propagation of elite plant
The certainly deficient important channel of horse spine germ plasm resource.
Due to geographical isolation and long-term natural selection, wild horse spine defines the aspects such as form, physiology, ecology, biochemistry
Genetic diversity, wild resource is gathered by quick method how, and keeps the excellent characteristic of maternal plant, be to carry out horse spine kind
Matter innovates the top priority of efficient utilization.But collection seed carries out breed and there are many deficiencies：On the one hand, resource is adjusted
Look into time not necessarily seed maturity；On the other hand, the seed of collection is difficult to keep maternal good characteristic.Collection is excellent
Branch carries out cutting propagation, and with the excellent characteristic that is little, can keeping maternal plant that makes a variation, thus it is fast and efficiently numerous to become horse spine
The important means grown.But fresh horse spine branch bar cuttage, branch root of hair ability are weaker, and rooting rate is relatively low, and survival rate is not
It is high.
The content of the invention
In order to solve the problems of the prior art, it is an object of the invention to provide a kind of vegetative method of horse spine.
The generation of plant cuttage propagation adventitious root primordia and the key that development is cuttage success or not, its formation depends on many
Kind of the factor, the present invention horse spine branch bar is processed by specific variable concentrations growth hormone, certain soak time after skewer
Insert, the rooting rate and survival rate of horse spine can be improved.
Unless otherwise specified, number of the present invention is weight portion, and the percentage ratio is mass percent.
The object of the present invention is achieved like this：
A kind of vegetative method of horse spine, including growth hormone is to horse spine branch bar immersion treatment and cuttage step, which is special
Levy and be：The one kind or several of the growth hormone in naphthalene acetic acid (NAA), heteroauxing (IBA), zeatin (Z), root-inducing powder
Plant combination.
An embodiment of the invention, it is 50 that above-mentioned growth hormone carries out immersion treatment for concentration to horse spine branch bar
The naphthalene acetic acid of～400mg/L soaks 1～24h to horse spine branch bar；It is dense that above-mentioned growth hormone carries out immersion treatment to horse spine branch bar
Spend the zeatin for 2.5～20mg/L and 1～24h is soaked to horse spine branch bar；Above-mentioned growth hormone is carried out at immersion to horse spine branch bar
Manage and 1～24h is soaked to horse spine branch bar for the root-inducing powder that concentration is 1.5～12g/L；Above-mentioned growth hormone is soaked to horse spine branch bar
Bubble is processed as the heteroauxing that concentration is 50～400mg/L and soaks 1～24h to horse spine branch bar.
Find in research, the naphthalene acid solution of certain concentration carries out the immersion of certain hour to fresh horse spine branch bar, can be with
Dramatically increase the survival rate of horse spine, rooting rate, total fresh quench weight.Variable concentrations and different soak times, the situation of taking root to horse spine
And growing state has larger difference.
Preferred concentration is that the naphthalene acetic acid of 50～400mg/L soaks 1～6h to horse spine branch bar；More preferably concentration is 50mg/L's
Naphthalene acetic acid soaks 1～6h to horse spine branch bar；The naphthalene acetic acid of 100mg/L soaks 3～6h to horse spine branch bar；The naphthalene acetic acid of 200mg/L
1～6h is soaked to horse spine branch bar.
For heteroauxing, process of the variable concentrations heteroauxing solution to horse spine difference soak time, its rooting rate,
Obvious difference between always fresh quench weight each process.Preferred concentration soaks 3～24h to horse spine branch bar for the heteroauxing of 100mg/L.
Application of the naphthalene acetic acid or heteroauxing of 50～400mg/L in horse spine cutting propagation.
For root-inducing powder, in variable concentrations rooting powder solution and solution in the process of different soak times, rooting rate is each
Significant difference between process, total root weight is with regard to significant difference between each time-triggered protocol.Preferred concentration is the root-inducing powder of 6～12g/L to horse spine
Branch soaks 3～24h.
Application of the root-inducing powder of 1.5～12g/L in horse spine cutting propagation.
Also find in research, in the process of different zeatin concentration and different soak time, rooting rate, total fresh quench weight with it is right
Not notable according to difference, impact of the zeatin solution to horse spine rooting rate is limited, but has been surprisingly found that certain concentration zeatin is processed
Horse spine branch bar survival rate it is higher, thus can consider fresh-keeping for branch.
On the other hand, concentration is application of the zeatin solution of 2.5～20mg/L in horse spine branch bar is fresh-keeping.Preferred concentration
Zeatin for 2.5～20mg/L soaks 1～3h to horse spine branch bar.
An embodiment of the invention, the vegetative method of above-mentioned horse spine, using following steps：
(1) branch is selected and is gathered
Select plant raw then or annual branch, gathered with shears when cloudy day or morning dew be not dry, will be cut
Branch put people's bucket in or with wet cloth, plastic sheeting wrap up, and transport to as early as possible branch processing process；
(2) fringe processed
First rinsed well with clear water before cutting processing, indoors or branch first is gone band is cut into after terminal bud by the place in outdoor cool place
The cutting of 4-5 lateral bud, length 10-15 centimetre, 0.5-1 centimetre above the section, lower cut cuts off down upper cut in the lower section of section
The blade of 2 lateral buds in portion, retains the blade on top；Produced cutting is put into into moisturizing in bucket during fringe processed；
(3) growth hormone immersion
Produced cutting is soaked in the potassium permanganate solution of 0.1%-0.5% sterilization in 1 hour, then, cutting base
Soak 6 hours in naphthalene acetic acid (NAA) solution of the concentration for 100mg/L in portion；
(4) cuttage
The cutting that growth hormone was soaked carries out cuttage in indoor flowerpot, and cutting bottom at least ensures a section in soil
In, water after cuttage, and keep ground moistening；After branch is survived, move in land for growing field crops together with basin soil.
Beneficial effect：
1st, the invention provides a kind of vegetative method of horse spine, growth hormone immersion of the method by certain concentration
Certain hour, then cutting propagation, the rootability and survival rate of horse spine are significantly improved.
2nd, in the process of different concentration of NAA difference soak time, rooting rate, total fresh quench have significant difference (P again<
0.01), except the branch of high concentration (200mg/L, 400mg/L) and long period of soaking (24h) is not taken root or (process NAA- of taking root on a small quantity
100-24) outward, the branch rooting rate that other were processed in naphthalene acid solution is above matched group.Process NAA-50-1, NAA-
50-3, NAA-100-3, NAA-200-3, NAA-50-6, the rooting rate of process NAA-100-6 are above 50%, wherein processing
The rooting rate highest of NAA-100-6, is 77.78%, secondly to process NAA-50-6, NAA-200-3, is all 66.67%.Process
Total fresh quench weight of NAA-100-6 is most heavy, is 4.176g, secondly to process NAA-50-3, is 3.616g.
3rd, wild horse spine branch cutting is in the process of different zeatin concentration and different soak time, rooting rate, total fresh
Root weight is not notable with contrast difference, but the branch that zeatin was processed is not dead, considers from cost and actual rooting efficiency,
Zeatin is not suitable for taking root for horse spine cuttage branch, but zeatin to can be used for branch fresh-keeping.Wild horse spine branch cutting
In variable concentrations heteroauxing solution and solution in the process of different soak times, the rooting rate highest of IBA-100-3 is processed,
For 55.56%；The total root weight for processing IBA-100-24 is most heavy, is 5.305g.Wild horse spine branch cutting is taken root in variable concentrations
In powder solution and solution in the process of different soak times, process the survival rate and rooting rate highest of root-inducing powder -12-3, all for
55.56%.And process root-inducing powder -3-1 and root-inducing powder -3-24 and all do not survive branch.
The analysis of each index of the 4th, horse spine branch bar being taken root by hormon, the branch that NAA was processed its rooting rate highest,
For 34.03%, total root weight of its all branch is also most heavy, is 24.106g, and most long root length is also most long, is 15.9cm.Next is made a living
Root powder, rooting rate are 20.14, but the total root of root-inducing powder does not have the branch weight that IBA was processed again.By the survival rate to branch, life
The measure of root amount and most long root length, as a result shows：Process NAA-100-6 survival rate height to be shown compared with other process, is taken root
The features such as rate is high, branch root of hair ability is strong.Can be with Preliminary conclusion：It is the preferable asexual propagation of horse spine to process NAA-100-6
Method.Horse spine branch bar survival rate can be effectively improved using the cottage method.
Specific embodiment
The present invention is specifically described below by specific embodiment, it is pointed out here that following examples are served only for this
It is bright to be further described, it is impossible to be interpreted as limiting the scope of the invention, the person skilled in the art of this area can be with root
Some nonessential modifications and adaptations are made to the present invention according to foregoing invention content.The all raw materials of the present invention and reagent are commercially available
Product.Wushan horse spine of the present invention is the wild horse spine for being grown in Wushan.
Embodiment
The vegetative method of horse spine, using following steps：
(1) cutting bed prepares
Needed using potted plant cuttage, plastic seeding culturing case width 40cm, long 80cm, high 22cm according to test.Load in nursery box
15cm high pearlite interstitial substance.
(2) bar and fringe processed are adopted
Mid-April, select plant raw then or annual branch, adopted with shears when not dry at cloudy day or morning dew
The branch cut is put in people's bucket or is wrapped up with wet cloth, plastic sheeting, and processed with transporting to branch processing as early as possible by collection；Cutting
First rinsed well with clear water before processing, indoors or branch first is gone to be cut into 4-5 lateral bud after terminal bud by the place in outdoor cool place
Cutting, length 10-15 centimetre, 0.5-1 centimetre above the section, lower cut cuts off 2 lateral buds in bottom in the lower section of section to upper cut
Blade, retains the blade on top；Produced cutting is put into into moisturizing in bucket during fringe processed；
(3) growth hormone immersion
Produced cutting is soaked in the potassium permanganate solution of 0.1%-0.5% sterilization in 1 hour, then, cutting base
Soak 6 hours in naphthalene acetic acid (NAA) solution of the concentration for 100mg/L in portion；
(4) cuttage
The cutting that growth hormone was soaked carries out cuttage in indoor flowerpot, with perlite as cutting medium, cutting bottom
At least ensure that a section, in soil, waters after cuttage, and keeps ground moistening；After branch is survived, move into together with basin soil
In land for growing field crops.
EXPERIMENTAL DESIGN
This test is taken root contrast test using hormon variable concentrations and different three factor of soak time, respectively with difference
The naphthalene acetic acid (NAA) of concentration, heteroauxing (IBA), zeatin (Z), 4 plant growth of root-inducing powder element, and branch is in each concentration
In solution, different soak times (1h, 3h, 6h and 24h) are processed, meanwhile, clear water is compared.Specific test process are shown in Table
1, NAA-50-1 is in naphthalene acetic acid (NAA) solution of 50mg/L to soak 1h, NAA-100-1, NAA-200-24 etc. by that analogy.
Using completely random district's groups test design method, respectively test each and process 30 cutting, be repeated 3 times.Jing is different
The cutting of concentration auxin solution process is inserted perpendicularly in substrate, cuttage seeding row spacing 5cm × 10cm, and cuttage depth is to insert at least
1 minor matters.Ground moistening is kept after cuttage.After 45 days cuttage seedling rootings, number of taking root is investigated, determine most long root length, root amount and simultaneously unite
Meter rooting rate.
1 different soaking concentrations of table and time test process method
Interpretation of result
1. the impact that naphthalene acetic acid (NAA) the different solutions concentration difference immersion treatment time takes root to cuttage branch
After wild Wushan horse spine branch cutting, 20d starts to take root, in the process of different concentration of NAA difference soak time
In, rooting rate, total fresh quench have significant difference (P again<0.01).The results are shown in Table 2, except high concentration (200mg/L, 400mg/L) and
The branch of long period of soaking (24h) is not taken root or taken root on a small quantity, and (process NAA-100-24) outward, other are processed in naphthalene acid solution
The branch rooting rate crossed is above matched group.Process NAA-50-1, NAA-50-3, NAA-100-3, NAA-200-3, NAA-50-
6th, the rooting rate for processing NAA-100-6 is above the not notable (P of difference between 50%, but each process>0.05), wherein, process NAA-
The rooting rate highest of 100-6, is 77.78%, secondly to process NAA-50-6, NAA-200-3, is all 66.67%.Process NAA-
Total fresh quench weight of 100-6 is most heavy, is 4.176g, secondly to process NAA-50-3, is 3.616g.
The impact that table 2 naphthalene acetic acid (NAA) the different solutions concentration difference immersion treatment time takes root to cuttage branch
2. the impact that zeatin (Z) the different solutions concentration difference immersion treatment time takes root to cuttage branch
Wild Wushan horse spine branch cutting is in the process of different zeatin concentration and different soak time, rooting rate, total
Fresh quench weight (P not notable with contrast difference>0.05).The rooting rate highest of Z-2.5-1, Z-2.5-3 is processed, is 33.33%, but
The branch that zeatin was processed is not dead, processes the survival rate 55.56% of Z-20-6, that is, the branch for having 55.56% is living
, but only 22.22% branch has sent out root.Therefore, consider that zeatin is not suitable for from cost and actual rooting efficiency
Wushan horse spine cuttage branch is taken root, but zeatin can consider fresh-keeping for branch.
The impact that table 3 zeatin (Z) the different solutions concentration difference immersion treatment time takes root to cuttage branch
3. the impact that heteroauxing (IBA) the different solutions concentration difference immersion treatment time takes root to cuttage branch
The process of wild Wushan horse spine branch cutting different soak times in variable concentrations heteroauxing solution and solution
In, the not notable (P of difference between rooting rate, each process of total fresh quench weight>0.05).The rooting rate highest of IBA-100-3 is processed, is
55.56%.The total root weight for processing IBA-100-24 is most heavy, is 5.305g.
The impact that table 4 heteroauxing (IBA) the different solutions concentration difference immersion treatment time takes root to cuttage branch
4. the impact that the root-inducing powder different solutions concentration difference immersion treatment time takes root to cuttage branch
Wild Wushan horse spine branch cutting in variable concentrations rooting powder solution and solution in the process of different soak times,
Significant difference (P between rooting rate each process<0.05), total root weight is with regard to significant difference (P between each time-triggered protocol<0.05).Process is taken root
The survival rate and rooting rate highest of powder -12-3, is all 55.56%.And processing root-inducing powder -3-1 and root-inducing powder -3-24 does not all have into
Branch living.
The impact that 5 root-inducing powder different solutions concentration of the table difference immersion treatment time takes root to cuttage branch
5. the impact that hormon is taken root to cuttage branch
The analysis (being shown in Table 6) of each index of Wushan horse spine branch bar being taken root by hormon, the branch that NAA was processed its life
Root rate highest, is 34.03%, and total root weight of its all branch is also most heavy, is 24.106g, and most long root length is also most long, is
15.9cm.Secondly it is root-inducing powder, rooting rate is 20.14, but the total root of root-inducing powder does not have the branch weight that IBA was processed again.Comprehensively examine
Consider each factor, each hormone on the size that branch root of hair affects is：NAA>Root-inducing powder>IBA>Z.
The impact that 6 hormon of table is taken root to horse spine branch bar
Conclusion：The species of hormone, concentration and soak time are all had an impact to Wushan horse spine branch bar rooting rate.By the test
Can draw, hormone selection NAA, concentration 100mg/L, branch soak time in the solution with 6 hours, give birth to by horse spine branch cutting
Preferably, rooting rate can effectively improve branch and breed survival rate up to 66.67% root effect.
Claims (10)
1. a kind of vegetative method of horse spine, including growth hormone is to horse spine branch bar immersion treatment and cuttage step, its feature
It is：The growth hormone is selected from naphthalene acetic acid（NAA）, heteroauxing（IBA）, zeatin（Z）, one or more in root-inducing powder
Combination；The growth hormone is soaked to horse spine branch bar for the naphthalene acetic acid that concentration is 50～400 mg/L to horse spine branch bar immersion treatment
1～24h, or the zeatin that concentration is 2.5～20 mg/L soaks 1～24h to horse spine branch bar, or concentration is 1.5～12 g/L
Root-inducing powder to horse spine branch bar soak 1～24h, or the heteroauxing that concentration is 50～400 mg/L to horse spine branch bar immersion 1～
24h。
2. the method for claim 1, it is characterised in that：The growth hormone to horse spine branch bar immersion treatment for concentration is
The naphthalene acetic acid of 50～400 mg/L soaks 1～6h to horse spine branch bar.
3. method as claimed in claim 2, it is characterised in that：The growth hormone is concentration 50 to horse spine branch bar immersion treatment
The naphthalene acetic acid of mg/L soaks 1～6h to horse spine branch bar；Or the naphthalene acetic acid of 100 mg/L of concentration soaks 3～6h to horse spine branch bar；Or
The naphthalene acetic acid of 200 mg/L of concentration soaks 1～6h to horse spine branch bar.
4. the method for claim 1, it is characterised in that：The growth hormone is concentration to horse spine branch bar immersion treatment
The zeatin of 2.5～20mg/L soaks 1～3h to horse spine branch bar.
5. the method for claim 1, it is characterised in that：The growth hormone is concentration 6 to horse spine branch bar immersion treatment
The root-inducing powder of～12 g/L soaks 3～24h to horse spine branch bar.
6. the method for claim 1, it is characterised in that：The growth hormone is concentration to horse spine branch bar immersion treatment
The heteroauxing of 100 mg/L soaks 3～24h to horse spine branch bar.
7. a kind of vegetative method of horse spine, using following steps：
（1）Branch is selected and is gathered
Select plant raw then or annual branch, gathered with shears when cloudy day or morning dew be not dry, by the branch cut
Wrap up during bar puts people's bucket or with wet cloth, plastic sheeting, and process with transporting to branch processing as early as possible；
（2）Fringe processed
First rinsed well with clear water before cutting processing, indoors or branch first is gone to be cut into after terminal bud with 4-5 by outdoor shady and cool place
The cutting of individual lateral bud, length 10-15 centimetre, 0.5-1 centimetre above the section, lower cut cuts off bottom 2 in the lower section of section to upper cut
The blade of individual lateral bud, retains the blade on top；Produced cutting is put into into moisturizing in bucket during fringe processed；
（3）Growth hormone soaks
Produced cutting is soaked into sterilization in 1 hour in the potassium permanganate solution of 0.1%-0.5%, then, cutting base portion is dense
Spend the naphthalene acetic acid for 100mg/L（NAA）Soak 6 hours in solution；
（4）Cuttage
The cutting that growth hormone was soaked carries out cuttage in indoor flowerpot, and cutting bottom at least ensures a section in soil,
Water after cuttage, and keep ground moistening；After branch is survived, move in land for growing field crops together with basin soil.
8. concentration is the application of the naphthalene acetic acid or heteroauxing of 50～400 mg/L in horse spine cutting propagation.
9. concentration is application of the zeatin of 2.5～20 mg/L in horse spine branch bar is fresh-keeping.
10. concentration is application of the root-inducing powder of 1.5～12 g/L in horse spine cutting propagation.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611039722.3A CN106538219A (en) | 2016-11-11 | 2016-11-11 | A kind of asexual reproduction method of Wild Legume shrub horse spine |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201611039722.3A CN106538219A (en) | 2016-11-11 | 2016-11-11 | A kind of asexual reproduction method of Wild Legume shrub horse spine |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107182532A (en) * | 2017-07-21 | 2017-09-22 | 重庆市畜牧科学院 | The asexual reproduction method of wild Buffalo bur |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101861792A (en) * | 2010-06-21 | 2010-10-20 | 青岛坤源农业科技开发有限公司 | Taxus media culture technology |
| CN105210774A (en) * | 2015-10-16 | 2016-01-06 | 徐发辉 | A kind of big fruit sea-buckthorn female plant hardwood cutting and seedling raising technical method |
-
2016
- 2016-11-11 CN CN201611039722.3A patent/CN106538219A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101861792A (en) * | 2010-06-21 | 2010-10-20 | 青岛坤源农业科技开发有限公司 | Taxus media culture technology |
| CN105210774A (en) * | 2015-10-16 | 2016-01-06 | 徐发辉 | A kind of big fruit sea-buckthorn female plant hardwood cutting and seedling raising technical method |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107182532A (en) * | 2017-07-21 | 2017-09-22 | 重庆市畜牧科学院 | The asexual reproduction method of wild Buffalo bur |
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