CN106495961A - The strong plantlets and rootage fertilizer that a kind of liquid fermentation production makes - Google Patents

The strong plantlets and rootage fertilizer that a kind of liquid fermentation production makes Download PDF

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Publication number
CN106495961A
CN106495961A CN201611187116.6A CN201611187116A CN106495961A CN 106495961 A CN106495961 A CN 106495961A CN 201611187116 A CN201611187116 A CN 201611187116A CN 106495961 A CN106495961 A CN 106495961A
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CN
China
Prior art keywords
fertilizer
bacillus
fruit
rootage
liquid fermentation
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Pending
Application number
CN201611187116.6A
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Chinese (zh)
Inventor
戴宝
卢德鹏
王树松
张焕刚
窦立志
刘泽凯
谭晓庆
丁克
冯学军
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WEIFANG XINDE BIOLOGICAL TECHNOLOGY CO LTD
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WEIFANG XINDE BIOLOGICAL TECHNOLOGY CO LTD
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Priority to CN201611187116.6A priority Critical patent/CN106495961A/en
Publication of CN106495961A publication Critical patent/CN106495961A/en
Pending legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80Soil conditioners
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B1/00Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
    • C05B1/02Superphosphates
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor

Abstract

The invention discloses the strong plantlets and rootage fertilizer that a kind of liquid fermentation production makes, makes after being sufficiently mixed by the raw material of following weight portion, bacillus subtilis, 10~15;Bacillus megaterium, 240~260;Kaolin, 190~210;Amino acid powder, 90~110;Humic acid, 90~110;Calcium magnesium phosphate, 90~110;White carbon, 4~6;Dodecyl sodium sulfate, 4~6;Zeolite powder, 90~110.The surface that this oranges and tangerines foliar fertilizer is sprayed onto Citrus leaf, the long-term dormancy of oranges and tangerines can be broken, promote oranges and tangerines to bloom, and coming off too early for fruit can be prevented after fruit is grown, improve the yield of fruit, the coat color of citrusfruit is vivid simultaneously, fruit of good quality, fruit freeze proof split and diseases prevention ability, and the sugar of fruit is lifted well, oranges and tangerines can units increased in production 6.5% or so, Gu acid than improve 40% or so.

Description

The strong plantlets and rootage fertilizer that a kind of liquid fermentation production makes
Technical field
The invention belongs to technical field of fertilizers, more particularly to be a kind of strong plantlets and rootage fertilizer that liquid fermentation production makes Material.
Background technology
The fragrant and sweet soft cunning of banana pulp, is one of fruit that people like.Banana contains the phosphorus of referred to as " salt of wisdom ", has again Rich in protein, sugar, potassium, vitamin A and C, while fiber is also more, it is one of often edible nutraceutical of people.Banana Pulp is per 100 grams containing sugar more than 15%, acid 0.2~0.3%, protein 1.5%, 53 milligrams of the phosphorus for also enriching, 19 milligrams of calcium, potassium 400 milligrams, 4 milligrams of Catergen.Banana is also containing pectin, multiple enzyme materials and trace element etc..
Banana is the crop of fast growing and high yield, and biomass is very big, except need a great number of elements such as nitrogen, phosphorus, potassium and calcium, magnesium, Outside the moderate-elements such as sulphur, in addition it is also necessary to the certain trace element of supplement, the effect of high yield and high quality could be obtained.Due to most of at present Banana producing region rainfall larger, soil application liquid fertilizer is easily lost in, it is difficult to absorb.For this purpose, Banana tree needs periodically to be applied Fertilizer.Although current strong plantlets and rootage fertilizer contains various bioactivators, energy activated plant vivo biodistribution hormone and biology enzyme, Promote crops unconventional take root, strong sprout, but its beneficiating ingredient species for containing not enough enriches, and particularly improves soil loosening the soil Aspect effect is poor, it is difficult to give full play of the maximum fertilizer efficiency of fertilizer, therefore also has very big room for improvement.
Content of the invention
The technical problem to be solved is to provide the strong plantlets and rootage fertilizer that a kind of liquid fermentation production makes.
For solving above-mentioned technical problem, the strong plantlets and rootage fertilizer that a kind of liquid fermentation production makes is the technical scheme is that Material, makes after being sufficiently mixed by the raw material of following weight portion:Bacillus subtilis, 10~15;Bacillus megaterium, 240~ 260;Kaolin, 190~210;Amino acid powder, 90~110;Humic acid, 90~110;Calcium magnesium phosphate, 90~110;White carbon, 4 ~6;Dodecyl sodium sulfate, 4~6;Zeolite powder, 90~110.
The fertilizer of taking root preferably, above-mentioned seedling-protection loosens the soil, makes after being sufficiently mixed by the raw material of following weight portion:Withered grass bud Spore bacillus, 12;Bacillus megaterium, 250;Kaolin, 200;Amino acid powder, 200;Humic acid, 100;Calcium magnesium phosphate, 100;In vain Carbon black, 5;Dodecyl sodium sulfate, 5;Zeolite powder, 100.
Preferably, the bacillus subtilis is 1,500,000,000 per gram, bacillus megaterium is 800,000,000 per gram.
Preferably, the bacillus subtilis are obtained by following methods:
A) prepared by seed:Strain idenfication meets characteristic, without variation, without miscellaneous bacteria;
B) culture medium is prepared:Weighing corn flour 1kg, ocean fish meal 0.5kg, groundnut meal 0.5kg adds 18kg water fully to dissolve; Then 10g, 20g, 5g, 100g, 15g are separately added into again(NH4)2SO4, CaCO3, KH2PO4, NaCI, MgSO4 7H2O, fills Appropriate defoamer is added after dividing dissolving, defoamer addition is with eliminating bubble;
C) ferment:Slack tank is sterilized before fermentation, 121~130 DEG C of sterilising temp is continually fed into steam, until tank pressure reaches 0.09 ~0.15Mpa, holds time 20~40 minutes, is down to 30 DEG C after cooling, culture medium is added slack tank then, and and is added aseptic Water adjusts cumulative volume, 5 times for culture medium weight of the weight of the water of addition, is subsequently adding Bacillus, addition according to Then 1% interpolation of culture medium weight portion, every gram of bacterial classification is cultivated containing bacillus 1.2~1.5 hundred million, and cultivation temperature is 29 ~31 DEG C, cultivate 36~40 hours, and keep pressure inside the tank in 0.05~0.08Mpa, pH is maintained between 6.5~7.0, and DO exists More than 25%, the culture when spore forming rate reaches 98% terminates, liquid bacterium number >=2.0 × 109cfu/ml, and is incubated step-down and obtains Nutrient solution;
D) it is spray-dried:Then nutrient solution is crossed 40 eye mesh screens, is spray-dried by the maltodextrin that nutrient solution is added 5%, EAT during spray drying is 160~170 DEG C, and leaving air temp is 80~85 DEG C, finally by the bacillus powder for obtaining 300 eye mesh screens are crossed, is packed.
As a result of above-mentioned technical proposal, the invention has the advantages that:
1st, rehabilitating soil, rich in the beneficial functions flora that can improve soil environment, such as bacillus subtilis, bacillus megaterium pine Native sterilized, and also grow containing other profitable strains in fertilizer while be the nutriment for plant growth, such as amino acid again Powder, humic acid, calcium magnesium phosphate etc., make beneficial bacterium in soil occupy absolute predominance, and can discharge the units such as phosphorus, potassium from soil Element, degrading organic phosphor pesticides are remained, and soil is significantly improved and is repaired.
2nd, fertilizer efficiency is improved, is used in mixed way with organic fertilizer, such as spreads fertilizer over the fields or spray on organic fertilizer, fertilizer can be promoted The decomposition of material, fully absorbs crop, improves fertilizer efficiency, uses cooperatively with npk fertilizer, and after such as applying azophoska, cooperation is sprayed Or drip irrigation this seedling-protection loosens the soil and takes root fertilizer solution on the surface of crop, can promote crop metabolism, have additional nutrients demand, improves nitrogen The effective rate of utilization of phosphorus potash fertilizer.
3rd, the fertilizer of taking root of this seedling-protection loosening the soil sprays or spreads fertilizer over the fields root or the blade face of banana, and banana tender leaf can be promoted to transcribe Aging, increase chlorophyll content, speeding-up blade grows, and makes any of several broadleaf plants seedling sturdy, and the speed of growth is accelerated;Any of several broadleaf plants shoot survival percent can be promoted to carry Height, promotes really full, the notable growth of any of several broadleaf plants, increases slightly, and single fruit fruit weight once adds, and improves the yield of any of several broadleaf plants fruit.
4th, culture medium main component is corn flour, ocean fish meal, groundnut meal, after fermenting through the organic thing liquid body of this mixing Product, in product, containing cellulose etc. is not difficult to the material of bacterial digestion absorption, and crude fat content is high, the effective energy value of fish meal Height, is a kind of high energy bacteria culture media;B family vitamins are rich in the fish meal of ocean, especially high with vitamin B12, B2 contents, also contain Contain the somatotrophic unknown factor in the liposoluble vitamins, and fish meal such as vitamin A, D and vitamin E, this material does not also have Compound is purified to, this material can stimulate the growth of bacterial classification.
Specific embodiment
The strong plantlets and rootage fertilizer that a kind of liquid fermentation production makes, makes after being sufficiently mixed by the raw material of following weight portion: Bacillus subtilis, 12;Bacillus megaterium, 250;Kaolin, 200;Amino acid powder, 200;Humic acid, 100;Calcium magnesium phosphate, 100;White carbon, 5;Dodecyl sodium sulfate, 5;Zeolite powder, 100;And bacillus subtilis is 1,500,000,000 per gram, huge gemma Bacillus is 800,000,000 per gram.
Wherein calcium magnesium phosphate is thoroughly mixed by following raw material and is formed:18 parts of ammonium hydrogen carbonate, 8 parts of calcium nitrate, 22 parts of calcium superphosphate, 13 parts of ground phosphate rock, 11 parts of bone meal, 4 parts of plant ash, 9 parts of calcium phosphate, 6 parts of phosphorus pentoxide, 5 parts of calcium silicates, 4 parts of magnesium silicate.
The bacillus subtilis are obtained by following methods:
A) prepared by seed:Strain idenfication meets characteristic, without variation, without miscellaneous bacteria;
B) culture medium is prepared:Weighing corn flour 1kg, ocean fish meal 0.5kg, groundnut meal 0.5kg adds 18kg water fully to dissolve; Then 10g, 20g, 5g, 100g, 15g are separately added into again(NH4)2SO4, CaCO3, KH2PO4, NaCI, MgSO4 7H2O, fills Appropriate defoamer is added after dividing dissolving, defoamer addition is with eliminating bubble;
C) ferment:Slack tank is sterilized before fermentation, 125 DEG C of sterilising temp is continually fed into steam, until tank pressure reaches 0.12Mpa, tieed up Hold the time 30 minutes, after cooling, be down to 30 DEG C, culture medium is added slack tank then, and and add sterilized water adjustment cumulative volume, plus The weight of the water for entering is 5 times of culture medium weight, is subsequently adding Bacillus, addition according to culture medium weight portion 1% Add, then every gram of bacterial classification is cultivated containing bacillus 1.2~1.5 hundred million, cultivation temperature is 30 DEG C, cultivates 36 hours, and Pressure inside the tank is kept in 0.065Mpa, pH is maintained at 7.0, DO more than 25%, and the culture when spore forming rate reaches 98% terminates, Liquid bacterium number >=2.0 × 109cfu/ml, and be incubated step-down and obtain nutrient solution;
D) it is spray-dried:Then nutrient solution is crossed 40 eye mesh screens, is spray-dried by the maltodextrin that nutrient solution is added 5%, EAT during spray drying is 160 DEG C, and leaving air temp is 80 DEG C, and the bacillus powder for obtaining is crossed 300 mesh sieves finally Net, is packed.
Seedling-protection of the present invention loosens the soil research of the fertilizer to banana qualitative effects of taking root
Test site:Gaozhou City of Guangdong Province Pingshan County experimental plot.
Test arranges 3 process:
1. spray this seedling-protection to loosen the soil fertilizer solution of taking root;
2. strength root-growing agent on the market, manufacturer are sprayed:Henan Shen Yu bio tech ltd,
Trade name:Strengthening root, high-effective root-growing powder, root are contained more,
Composition:Plant growth promoter, cell division reinforcing agent, Nafusaku, indolebutyric acid etc.
Physicochemical property:This product for white to buff powder, soluble in water, be dissolved in the organic solvents such as ethanol, methyl alcohol,
Formulation:98% pulvis
Packaging:1kg/ bags, 25kg/ buckets,
3. clear water.
The kind of test is Guangdong 63-1, is planted using plantlet in vitro in March, 2014, is spaced in May and sprays twice This seedling-protection loosens the soil 1000 times of fertilizer solution of taking root, and control treatment then sprays clear water simultaneously, and another set spray strength on the market is taken root Agent, strength root-growing agent consumption are applied according to its packing instruction.
By above test, and through contrast, finally show that loosen the soil fertilizer solution of taking root of this seedling-protection is contrasted with clear water, averagely Make Jiao Miaoduo grow 0.92 leaf, averagely make any of several broadleaf plants seedling increase 6.15 centimetres, averagely increase any of several broadleaf plants stem thick 0.83 centimetre, reach notable water Flat, and chlorophyll content contrast clear water, value added about 0.352mg/g, equivalent to 31.82%, and with the market strong that participates in the experiment Power root-growing agent chlorophyll value added has 0.24mg/g, and equivalent to 19.1%, loosen the soil effect of fertilizer of taking root of this seedling-protection is superior on the market Strength root-growing agent.
Schematically specific embodiment of the invention is the foregoing is only, the scope of the present invention is not limited to.Any Those skilled in the art, done equivalent variations and modification on the premise of the design without departing from the present invention and principle, The scope of protection of the invention should be belonged to.

Claims (4)

1. the strong plantlets and rootage fertilizer that a kind of liquid fermentation production makes, it is characterised in that fully mixed by the raw material of following weight portion Make after conjunction:Bacillus subtilis, 10~15;Bacillus megaterium, 240~260;Kaolin, 190~210;Amino acid powder, 90~110;Humic acid, 90~110;Calcium magnesium phosphate, 90~110;White carbon, 4~6;Dodecyl sodium sulfate, 4~6;Zeolite Powder, 90~110.
2. the strong plantlets and rootage fertilizer that a kind of liquid fermentation production makes as claimed in claim 1, it is characterised in that by following heavy The raw material of amount part is made after being sufficiently mixed:Bacillus subtilis, 12;Bacillus megaterium, 250;Kaolin, 200;Amino acid Powder, 200;Humic acid, 100;Calcium magnesium phosphate, 100;White carbon, 5;Dodecyl sodium sulfate, 5;Zeolite powder, 100.
3. the strong plantlets and rootage fertilizer that a kind of liquid fermentation production makes as claimed in claim 1, it is characterised in that:The withered grass Bacillus is 1,500,000,000 per gram, and bacillus megaterium is 800,000,000 per gram.
4. a kind of strong plantlets and rootage fertilizer that liquid fermentation production makes as claimed in claim 1, it is characterised in that the withered grass Gemma bar is obtained by following methods:
A) prepared by seed:Strain idenfication meets characteristic, without variation, without miscellaneous bacteria;
B) culture medium is prepared:Weighing corn flour 1kg, ocean fish meal 0.5kg, groundnut meal 0.5kg adds 18kg water fully to dissolve; Then 10g, 20g, 5g, 100g, 15g are separately added into again(NH4)2SO4, CaCO3, KH2PO4, NaCI, MgSO4 7H2O, fills Appropriate defoamer is added after dividing dissolving, defoamer addition is with eliminating bubble;
C) ferment:Slack tank is sterilized before fermentation, 121~130 DEG C of sterilising temp is continually fed into steam, until tank pressure reaches 0.09 ~0.15Mpa, holds time 20~40 minutes, is down to 30 DEG C after cooling, culture medium is added slack tank then, and and is added aseptic Water adjusts cumulative volume, 5 times for culture medium weight of the weight of the water of addition, is subsequently adding Bacillus, addition according to Then 1% interpolation of culture medium weight portion, every gram of bacterial classification is cultivated containing bacillus 1.2~1.5 hundred million, and cultivation temperature is 29 ~31 DEG C, cultivate 36~40 hours, and keep pressure inside the tank in 0.05~0.08Mpa, pH is maintained between 6.5~7.0, and DO exists More than 25%, the culture when spore forming rate reaches 98% terminates, liquid bacterium number >=2.0 × 109Cfu/ml, and be incubated step-down and obtain Nutrient solution;
D) it is spray-dried:Then nutrient solution is crossed 40 eye mesh screens, is spray-dried by the maltodextrin that nutrient solution is added 5%, EAT during spray drying is 160~170 DEG C, and leaving air temp is 80~85 DEG C, finally by the bacillus powder for obtaining 300 eye mesh screens are crossed, is packed.
CN201611187116.6A 2016-12-21 2016-12-21 The strong plantlets and rootage fertilizer that a kind of liquid fermentation production makes Pending CN106495961A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107056442A (en) * 2017-04-13 2017-08-18 诸城常山永辉生态农业家庭农场 A kind of anti-continuous cropping fertilizer

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102258059A (en) * 2011-07-13 2011-11-30 山东玉成生化农药有限公司 Method for preparing ralstonia solanacearum-resistant bacillus subtilis microbial inoculum
CN102627514A (en) * 2012-04-27 2012-08-08 深圳市芭田生态工程股份有限公司 Soil-acidification-resistant organic fertilizer additive and preparation method thereof
CN103288536A (en) * 2013-05-29 2013-09-11 王龙潮 Probiotic wettable powder fertilizer
CN103553814A (en) * 2013-11-08 2014-02-05 苏州仁成生物科技有限公司 Nano sustained-release fertilizer prepared by multiple fermentation and preparation method thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102258059A (en) * 2011-07-13 2011-11-30 山东玉成生化农药有限公司 Method for preparing ralstonia solanacearum-resistant bacillus subtilis microbial inoculum
CN102627514A (en) * 2012-04-27 2012-08-08 深圳市芭田生态工程股份有限公司 Soil-acidification-resistant organic fertilizer additive and preparation method thereof
CN103288536A (en) * 2013-05-29 2013-09-11 王龙潮 Probiotic wettable powder fertilizer
CN103553814A (en) * 2013-11-08 2014-02-05 苏州仁成生物科技有限公司 Nano sustained-release fertilizer prepared by multiple fermentation and preparation method thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107056442A (en) * 2017-04-13 2017-08-18 诸城常山永辉生态农业家庭农场 A kind of anti-continuous cropping fertilizer

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Application publication date: 20170315

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