CN106490052B - Compound spray and its application in promotion crop kernel grouting and seed vitality - Google Patents
Compound spray and its application in promotion crop kernel grouting and seed vitality Download PDFInfo
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- CN106490052B CN106490052B CN201610817301.2A CN201610817301A CN106490052B CN 106490052 B CN106490052 B CN 106490052B CN 201610817301 A CN201610817301 A CN 201610817301A CN 106490052 B CN106490052 B CN 106490052B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/02—Amines; Quaternary ammonium compounds
- A01N33/04—Nitrogen directly attached to aliphatic or cycloaliphatic carbon atoms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/44—Poaceae or Gramineae [Grass family], e.g. bamboo, lemon grass or citronella grass
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Abstract
The invention discloses compound spray and its promoting the application in crop kernel grouting and seed vitality, which is made of the component A and B component of volume ratio 1:1~100；The component A takes water as a solvent, and includes: 1~50mM of spermidine, 1~15mM of methyl jasmonate, 10~50mM of ethyl alcohol；The B component is corn children fringe leaching liquor.The present invention is using spermidine and methyl jasmonate as the effective component of spray, mix corn children fringe leaching liquor, blade face or the female fringe of crop are sprayed with debita spissitudo, not only effectively promote the dry-matter accumulation of corn seed, corn mass of 1000 kernel is increased, and improves the seed vitality after harvest.
Description
Technical field
The present invention relates to crop production technology field more particularly to compound spray and its promote crop kernel grouting and
Application in seed vitality.
Background technique
Corn is also known as vegetable corn, is one of faster new vegetable variety of development in recent years.
Corn because have the characteristics that nutrition abundant and sweet tea, it is fresh, crisp, tender due to deep liked by each stratum consumer.In recent years
Come, planting of sweet corn scale keeps surely increasing situation year by year, and constantly substantial increase, breeding of new varieties further mention export volume
Speed, postpartum process scale go from strength to strength, and planting income keeps the impetus favourable.But sweet corn seeds poor quality, it is serious to hinder
The development of corn industry.Currently, the sweet corn seeds market price is expensive, monoseeding is the development side of Cultivation mode
To the sweet corn seeds of high vigor can realize that abridged edition increases production while realizing simple grain precision drilling.It is compared with conventional corn, sweet tea
Vigor of Zea mays L. Seeds is lowly mainly that itself repertory source as caused by starch deficiency, imbibition damage and germ infection etc. is supplied
Caused by scarce capacity.
Polyamines is generally existing in higher plant, is a new class of important physiological activator.Putrescine, spermidine and essence
Amine is three kinds of most common polyamines, participate in the various growth and development processes of plant, can be improved plant to high salt, low temperature, ozone and
The repellence of the adverse circumstances such as potassium deficiency.Currently, have proven to polyamines and metabolism with bloom bear fruit, fruit quality, embryo development and seed matter
It measures closely related.
Application publication number is that the application for a patent for invention document of CN104012534A discloses a kind of wheat cold-resistant regulator, should
The proportion of regulator are as follows: sea grass polysaccharide 100-1000mg/L, salicylic acid 0mg/L or 100-200mg/L, brassinosteroid 0mg/L
Or 0.01-0.1mg/L, glycine betaine 0mg/L or 100-1000mg/L, spermidine 0mg/L or 10-100mg/L, surfactant
0mg/L or 1000-20000mg/L, water is as solvent；Sea grass polysaccharide is the low molecular weight sea grass polysaccharide of molecular weight 1k-50kDa；
The surfactant being related to is one of TWEEN Series, Span series, OP series and/or NP series or a variety of mixtures.
The regulator component is simple, and raw material is easy to get, low in cost, significant effect, and environmental-friendly.
In existing sweet corn seeds production process, mainly adjusted by genetic improvement, maturity control, holding conditions
Sweet corn seeds vigor is improved with seed processing technology etc..The application selects a kind of compound during the corn production of hybrid seeds
Hormone sprays reagent to promote accumulation of seed's dry matter, and improves seed vitality, and at home and abroad there is not been reported for this.
Summary of the invention
The present invention provides a kind of compound spray and its promoting the application in crop kernel grouting and seed vitality, it should
Compound spray can not only promote the dry-matter accumulation of crop kernel, increase crop mass of 1000 kernel, additionally it is possible to improve seed vitality.
A kind of compound spray is made of the component A and B component of volume ratio 1:1~100；
The component A takes water as a solvent, and includes: 1~50mM of spermidine, 1~15mM of methyl jasmonate, and ethyl alcohol 10~
50mM；
The B component is corn children fringe leaching liquor.
In above-mentioned compound spray, methyl jasmonate is a kind of plant endogenous growth substance, and spermidine can be stimulated to synthesize base
The expression of cause, and then promote the synthesis of plant endogenous spermidine.
Corn children's fringe refers to 5~10 days after pollination maize ears.
Specifically, the preparation method of the component A, comprising: methyl jasmonate is added in ethyl alcohol, stirring and dissolving is made into
Methyl jasmonate mother liquor；Spermidine and methyl jasmonate mother liquor are added to the water, stirred evenly to get component A；
Preferably, the volume ratio of the component A and B component is 1:1~50.It is further preferred that the component A and B component
Volume ratio is 1:20.
Specifically, the preparation method of the corn children fringe leaching liquor includes: to air-dry corn children's fringe, obtain after crushing
Powder be placed in water extraction, remove insoluble matter, obtain corn children fringe leaching liquor.
Preferably, the additive amount of the powder is 30~100g/L.
Preferably, the temperature of the extraction is 25~35 DEG C, the time is 3~5 days.
The present invention also provides the compound sprays to promote the application in crop kernel grouting.
The present invention also provides the compound sprays to promote the application in Vigor of Crop.
Specifically, the plant is corn.
The application, comprising: the compound spray is uniformly sprayed on three leaf of plant female fringe and fringe.
Preferably, the compound spray spraying time are as follows: 1~3 day afternoon after corn pollination.
Preferably, the dosage of the compound spray is 100~500L/ mus.
Compared with prior art, the invention has the following advantages:
(1) present invention is using spermidine and methyl jasmonate as the effective component of spray, mixing corn children fringe extraction
Liquid is sprayed on blade face or the female fringe of crop with debita spissitudo, not only effectively promotes the dry-matter accumulation of corn seed, increases
Add corn mass of 1000 kernel, and improves the seed vitality after harvest.
(2) for the compound spray of the present invention using water as matrix, preparation process is simple, easy to use, will not generate product and ring
Border pollution, is not likely to produce phytotoxicity, has broad application prospects.
Specific embodiment
In following embodiment, corn Parent self-mating system comes from Zhejiang University's seed scientific center；Spermidine and jasmine
Sour methyl esters is purchased from Aladdin reagent Co., Ltd；Magnetic stirring apparatus model ZNCL-BS has purchased from the more instrument and equipments in Shanghai
Limit company.
Embodiment 1
One, the preparation of compound spray
The preparation of component A: 2.24g methyl jasmonate and 5ml ethyl alcohol are mixed, and are sufficiently dissolved, and methyl jasmonate mother is obtained
Liquid；5ml methyl jasmonate mother liquor and 2.76g spermidine are added in 995ml water, dissolution is sufficiently stirred, obtains component A.
The preparation of component B: corn children's fringe is air-dried, and is ground to obtain the powder of 0.10~0.15mm (partial size), is taken 200g
Powder be placed in the water of 4L and impregnate 3 days at room temperature after, filter off insoluble matter, obtain component B.
Component A and component B is mixed by 1:20 (v/v), obtains compound spray.
Two, compound spray sprays
1, material to be tested: inbred line of sweet corn T49-2-6-1-1 (♀) and S29-6-2-3 (♂)
2, reagent agent: compound spray
3, test method
Before maternal fruit ear reels off raw silk from cocoons, bagging is carried out, male parent carries out artificial pollination after taking out male flowering, obtains the hybridization of the material
F1Seed.Growth period carries out consistent field management.
Spray handles time and method: 1 day after pollination, 4 kinds of spray processing, respectively spermidine (Spd) are arranged
(concentration is identical as Spd concentration in A processing), A (component A/water=1/20, v/v), B (water/B component=1/20, v/v), A+B (A
Component/B component=1/20, v/v).Three leaf of fringe is uniformly sprayed according to the dose of 252L per acre, control sprays equivalent clear water.
Sampling method: 26 days after pollination, being sampled, each to handle the corn kernel for taking female fringe middle section.
The long generous, volume of sweet corn seeds and dry weight and fresh weight of plant seedlings measurement: 3 repetitions, 10 kinds of each repetition are arranged in each processing
Son.Seed length and width and thickness are measured with ruler, is accurate to 0.01cm, seed volume is measured with graduated cylinder, is weighed with one thousandth balance
Seed fresh weight, and dried in 80 DEG C of baking ovens and claim its dry weight afterwards for 24 hours, it is accurate to 0.01g.
4. test result
26 days after pollination, the thickness and volume of compound spray (A+B) processing seed are significantly higher than other 4 processing, long
It is handled with other without significant difference with wide；The fresh weight and dry weight of compound spray (A+B) processing seed are significantly higher than at other 4
It manages (table 1), it is seen that compound spray can remarkably promote expanding for sweet corn seeds volume, promote dry matter during Grain Development
Accumulation improves mass of 1000 kernel, increases yield.
1 different disposal of table is to long generous, dry weight and fresh weight of plant seedlings, volume the influence of 26 days sweet corn seeds after pollination
* the significance of difference between lowercase expression processing in α=0.05 (LSD test) level.
Embodiment 2
One, the preparation of compound spray
4 groups of sprays processing of the present embodiment design and 1 group of control treatment are same as Example 1.
Two, compound spray sprays
1, material to be tested: inbred line of sweet corn T49-2-6-1-1 (♀) and S29-6-2-3 (♂) hybridizes F1 seed.
2, test method
Seed germination experiment: germination test is carried out to the seed of 26 days harvests after pollination.Referring to ISTA inspection procedure, use
6 × 12cm moistens paper roll germination, 4 repetitions, 100 seeds of every repetition.Seed is placed in 25 DEG C of constant incubators, illumination setting
It is dark for 12h illumination/12h.1mm is broken in the seed coat as germination standard so that seed base-root is prominent, germinative number is recorded daily, in germination the 4th day
Germinating energy and germination percentage were calculated separately with the 7th day.
Germination index GI=∑ (Gt/Tt), wherein Gt is t days germinative numbers, and Tt is number of days.
Vitality index VI=GI × seedling stem weight.In the 7th day measurement the seedling root long, height of seedling, dry weight and fresh weight of plant seedlings of germinateing.
The measurement of sweet corn seeds leachate relative conductivity: sweet corn seeds 0.2g is taken, is washed with deionized water, tool is put into
It fills in test tube, deionized water 15ml is added, stand 1h, measure conductivity value (E with DDS-11A type conductivity gauge1).Then boiling water bath
30min is handled, conductivity (E is measured2), the conductivity value of deionized water is E0。
Then relative conductivity is calculated: REL=(E1-E0)/(E2-E0) × 100%
3, test result
The percentage of seedgermination of compound spray (A+B) processing is handled with other 4 without significant difference, but its germinating energy will be shown
It writes and is higher than control, while its germination index and vitality index will be significantly higher than other 4 processing (table 2), illustrate compound spray
(A+B) processing significantly improves the germinating capacity of sweet corn seeds.
Compared with other 4 processing, it is relatively electric that compound spray (A+B) processing significantly reduces sweet corn seeds leachate
Conductance shows that compound spray processing has certain protective effect to sweet corn seeds cell membrane integrity.
Compared with the control, compound spray (A+B) handles while significantly improving root long, seedling stem after corn germinates 7 days
Weight and root dry weight (table 3), and promote benefit throughout manage in put up the best performance, sweet tea can be effectively improved by illustrating that compound spray is handled
Seedling quality after corn seed germination.
Influence of 2 different disposal of table to 26 days sweet corn seeds germinations after pollination
* the significance of difference between lowercase expression processing in α=0.05 (LSD test) level.
Influence of 3 different disposal of table to 26 days sweet corn seeds germination seedling qualities after pollination
* the significance of difference between lowercase expression processing in α=0.05 (LSD test) level.
Claims (9)
1. a kind of compound spray, which is characterized in that be made of the component A and B component of volume ratio 1: 1 ~ 100；
The component A takes water as a solvent, and includes: 1~50 mM of spermidine, 1~15 mM of methyl jasmonate, ethyl alcohol 10~50
mM；
The B component is corn children fringe leaching liquor；
The preparation method of the corn children fringe leaching liquor include: after corn children's fringe is air-dried, is crushed the powder that obtains be placed in
It is extracted in water, removes insoluble matter, obtain corn children fringe leaching liquor.
2. compound spray as described in claim 1, which is characterized in that the volume ratio of the component A and B component is 1: 1 ~
50。
3. compound spray as described in claim 1, which is characterized in that the additive amount of the powder is 30 ~ 100 g/L.
4. compound spray as described in claim 1, which is characterized in that the temperature of the extraction is 25 ~ 35 DEG C, the time is 3 ~
5 days.
5. the compound spray as described in claim 1 ~ 4 is any is promoting the application in crop kernel grouting.
6. the compound spray as described in claim 1 ~ 4 is any is promoting the application in Vigor of Crop.
7. such as application described in claim 5 or 6 characterized by comprising the compound spray is uniformly sprayed on plant
On three leaf of female fringe and fringe.
8. the use as claimed in claim 7, which is characterized in that the compound spray spraying time are as follows: 1 after corn pollination
~ 3 days afternoons.
9. the use as claimed in claim 7, which is characterized in that the dosage of the compound spray is 100 ~ 500 L/ mus.
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| CN201610817301.2A CN106490052B (en) | 2016-09-12 | 2016-09-12 | Compound spray and its application in promotion crop kernel grouting and seed vitality |
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| CN107135758A (en) * | 2017-03-27 | 2017-09-08 | 苏州大学 | A kind of method that utilization Exogenous Putrescine Application improves red palm cold resistance |
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| CN109511657B (en) * | 2018-11-22 | 2021-01-15 | 内蒙古农业大学 | Method for improving corn yield |
| CN112400629A (en) * | 2020-11-23 | 2021-02-26 | 安徽农业大学 | Method for improving seed production quality of non-heading Chinese cabbages |
| CN115997765A (en) * | 2022-11-29 | 2023-04-25 | 中国农业大学 | Spermidine-containing plant germination accelerator and application thereof in promoting germination of plant seeds |
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