CN110720277A - Application of hydrogen sulfide donor sodium hydrosulfide in increasing crop yield and improving nutritional quality - Google Patents
Application of hydrogen sulfide donor sodium hydrosulfide in increasing crop yield and improving nutritional quality Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/40—Fabaceae, e.g. beans or peas
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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Abstract
The invention discloses an application of hydrogen sulfide donor sodium hydrosulfide in improving crop yield and improving nutritional quality, and particularly relates to a method for improving crop yield and improving nutritional quality by preparing sodium hydrosulfide into a sodium hydrosulfide aqueous solution with the concentration of 0.01 ~ 0.8.8 mmol/L, soaking roots of the sodium hydrosulfide aqueous solution with different concentration gradients from the seedling stage of crops, and spraying plants with a sodium hydrosulfide Tween solution with the concentration of 0.01 ~ 0.8.8 mmol/L in the flowering stage of the crops.
Description
Technical Field
The invention relates to the technical field of crop planting, in particular to a method for improving crop yield and improving nutritional quality by using hydrogen sulfide, and belongs to the field of plant growth regulators.
Background
The vegetables are the crops with the widest cultivation area and the most important economic status except the grain crops in China. In recent years, the rapid development of socioeconomic performance in China causes the corresponding changes of dietary structure, life style and disease spectrum. The vegetable is used as an essential subsidiary food in daily life, and provides abundant nutrient substances such as carbohydrate, vitamin, cellulose and the like for people. With the improvement of living standard, the demand of people for leaf vegetables is continuously increasing. The leaf vegetables are a large category of vegetables and have high nutritional value. Due to higher economic value and demand, the planting area is also increased continuously. The traditional production mode of the leaf vegetables in China cannot meet the market demand, so that the improvement of the yield and the quality of the leaf vegetables is urgently needed.
The bean crops are one of the important economic crops in China and also are the main sources for obtaining proteins by people. It is an important grain crop for the agricultural development of China at present. It is rich in nutrients such as vitamins, fat and protein. Legume crops not only contain large amounts of proteins, but also contain a very large number of amino acids, particularly lysine, which are the main source of protein. At present, bean crops gradually become necessities of daily life of people, and along with the increasing demand of people on the bean crops, the improvement of the nutritional quality of the crops and the improvement of the crop yield are particularly important. The bean crops are rich in nutrient elements necessary for human growth, and the increasing demands of people can be met only by continuously improving the nutritional quality of the crops and increasing the yield of the bean crops.
H2S has gained wide acceptance as an important signaling molecule in plants. The change of H2S level in plants plays an important role in researching the function of H2S in plants. H2S, like NO and CO, is an important gas signaling molecule that can be involved in regulating various physiological processes in animal cells at low concentrations. In recent years, H2S has received more and more attention in the field of plant research, and it is found that the signal molecule is involved in many processes for regulating and controlling plant growth and development, such as increasing seed germination rate, regulating and controlling stomatal movement, relieving drought osmotic stress, salt stress, heat shock and heavy metal damage to plants, promoting the biogenesis of adventitious roots, delaying plant senescence, prolonging flowering phase, promoting fruit preservation and the like. It was found that H2S promotes the synthesis of plant chlorophyll and regulates stomatal movement in a dose-dependent manner, suggesting that H2S may be involved in regulating plant photosynthesis as well as NO and CO signaling molecules.
NaHS, as a donor for H2S, has been widely used in the field of zoology research. NaHS, when dissolved in water, ionizes to produce Na + and HS-, which then combine with H + to form H2S. Research shows that the content of chlorophyll in plant leaves can be effectively increased by exogenously adding H2S, and NaHS can enhance photosynthesis in a dose-dependent mode so as to promote the growth of seedlings.
Until now, sodium hydrosulfide has been studied for improving crop stress resistance, and has been used as a plant pollen germination promoter to inhibit the abscission of plant organs, but the research on increasing crop photosynthetic rate, promoting crop growth and development, increasing crop yield and improving nutritional quality by using hydrogen sulfide donor sodium hydrosulfide is relatively less.
Disclosure of Invention
In order to solve the defects of the prior art, the invention provides an application method of a hydrogen sulfide donor sodium hydrosulfide in improving crop yield and improving nutritional quality.
In order to achieve the above object, the present invention adopts the following technical solutions:
use of a hydrogen sulfide donor sodium hydrosulfide for increasing crop yield and improving nutritional quality.
Further, the crops comprise leaf vegetable crops and bean crops.
Furthermore, accelerating germination and culturing seeds of the leaf vegetables, and soaking roots by using a sodium hydrosulfide solution with the concentration of 0 ~ 0.8.8 mmol/L when the crops grow to have two leaves and one heart.
Further, the specific method for pregermination culture comprises the following steps:
a. accelerating germination of seeds of leaf vegetables at the temperature of 22 ~ 25 ℃;
b. seedlings after growing one leaf and one heart are transferred to Hoagland nutrient solution for growth under the conditions that the light/dark period is 16/8 h and the temperature is 20 ~ 26 ℃.
Further, the concentration of the sodium hydrosulfide solution is 0.01 ~ 0.2.2 mmol/L.
Further, seeds of the leguminous crops are planted and cultured, when the leguminous crops grow to the seedling stage, root irrigation treatment is carried out on the leguminous crops by using a sodium hydrosulfide solution with the concentration of 0 ~ 0.8.8 mmol/L, and when the leguminous crops grow to the flowering and pod stage, the leaf surfaces of the leguminous crops are sprayed by using a sodium hydrosulfide and tween mixed solution.
Further, the concentration of the sodium hydrosulfide solution is 0.05 ~ 0.2.2 mmol/L.
Furthermore, the sodium hydrosulfide and tween mixed solution is prepared by uniformly mixing 0.01 ~ 0.8.8 mmol/L sodium hydrosulfide solution and tween-80, and the tween-80 is a hydrophilic surfactant.
Compared with the prior art, the invention has the beneficial effects that:
1. the reagent of the invention has wide source of sodium hydrosulfide, combines the growth characteristics of crops and selects proper concentration of sodium hydrosulfide solution, thereby avoiding the damage to the crops and promoting the growth and development of the crops.
2. The invention has obvious effect and low cost, is suitable for large-scale application and has good economic benefit and social value.
3. The invention can effectively improve the yield of crops and the quality of the crops, and provides a new way for improving the yield of the crops and the nutritional quality.
4. The sodium hydrosulfide used as a signal molecule hydrogen sulfide provider has low exogenous application concentration and no pollution to the environment.
5. The sodium hydrosulfide aqueous solution is simple to prepare, the sodium hydrosulfide is directly dissolved in water, and the method is easy to operate.
6. The sodium hydrosulfide aqueous solution is characterized in that the sodium hydrosulfide slowly releases hydrogen sulfide in water to play a role in promoting photosynthesis of crops.
Drawings
FIG. 1 is a graphical representation of the sodium hydrosulfide increasing "American Rapid Heat resistant" lettuce biomass profile of example 1;
FIG. 2 is a table diagram of the sodium hydrosulfide improving the biomass of the Chinese cabbage;
FIG. 3 is a graphical representation of the sodium hydrosulfide enhanced biomass profile of "Suzhou green" oilseed rape of example 3;
FIG. 4 is a table diagram of example 4 showing that sodium hydrosulfide improves the biomass of "four seasons" chinese little greens;
FIG. 5 is a graph of the fruit grain size of the "big pods 618" pea fruit increased by sodium hydrosulfide of example 5;
FIG. 6 is a graph of the fruit grain size of sodium hydrosulfide increasing the yield of "RL 109" peas of example 6;
FIG. 7 is a graph of the grain size of the fruits of mung beans of "Sukang No. 3" improved by sodium hydrosulfide in example 7.
Detailed Description
The present invention will be described in further detail with reference to examples, but the present invention is not limited thereto.
The reagent used in the present invention was NaHS, purchased from Sigma, as a H2S donor. The sodium hydrosulfide is solid and needs to be stored in a sealed, shady and cool place away from light, so as to avoid contact with oxidant and acid, and is not suitable for long-term storage so as to avoid deterioration; the sodium hydrosulfide aqueous solution should be prepared as it is.
Example 1
The application of sodium hydrosulfide as hydrogen sulfide donor in raising the yield of heat-resisting lettuce and improving the nutritive quality in America.
The seeds need to be subjected to pregermination treatment at the temperature of 22 ~ 25 ℃, seedlings after one leaf and one heart are grown are transferred to Hoagland nutrient solution (Hoagland nutrient solution) to grow under the conditions that the light/dark period is 16/8 h, the temperature is 20 ~ 26 ℃, and the root of the American fast heat-resistant lettuce is soaked by a sodium hydrosulfide solution with the concentration of 0 ~ 0.8.8 mmol/L (roots are completely soaked) from the period of two leaves and one heart.
The method comprises the following specific steps:
1. selecting plump and uniform seeds, washing with tap water for 3 times to remove impurities in the seeds, sterilizing with 5% sodium hypochlorite aqueous solution for 10 ~ 15 min, washing with tap water for 20 ~ 30 min to completely remove sodium hypochlorite;
2. after the seeds germinate, selecting uniform and consistent seeds, putting the seeds in a culture dish, culturing the seeds with deionized water, and replacing the deionized water every 12 ~ 24 hours until the seeds are cultured to be one leaf and one core;
3. under the temperature condition of 25 ~ 30 ℃ and 30 ℃, the seedlings with uniform growth vigor are cultured by Hoagland nutrient solution, namely, water culture planting, and each water culture box contains 300 ~ 700ml of nutrient solution;
4. weighing 0.056 g of sodium hydrosulfide, dissolving the sodium hydrosulfide into 10mL of deionized water to prepare a mother solution A with the concentration of 100 mmol/L, adding the mother solutions A with different volumes into a water culture box according to a certain proportion to dilute the mother solutions A into sodium hydrosulfide solutions with different concentrations of 0 ~ 0.8.8 mmol/L, carrying out root soaking treatment on crops (immersing the roots completely) by using the sodium hydrosulfide solution with the concentration of 0 ~ 0.8mmol/L from the first two leaves in the first heart period, continuously treating for 2 ~ 5 times, replacing the treatment solution once every 12 ~ 24h, then culturing for 72 ~ 120 h by using Hoagland nutrient solution, and replacing the nutrient solution once every 24 ~ 48h, wherein the treatment is a period, and the treatment period is 5 ~ 10 days.
5. Thereafter, the culture of step 4 was repeated for 4 ~ 8 continuous cycles.
In the embodiment, American big-speed heat-resistant lettuce is adopted to carry out experiments of increasing crop yield and improving nutritional quality by using sodium hydrosulfide as a hydrogen sulfide donor, the fresh weight of the picked American big-speed heat-resistant lettuce is weighed by an electronic balance, and lettuce leaves are collected to determine the content of chlorophyll and soluble protein. The experiment is repeated three times, and the content of chlorophyll and protein in the leaf at the same position of the lettuce in the sample is measured each time. The results are shown in table 1 and are expressed as "mean ± standard deviation", and the difference between the treatment and control groups was analyzed using the Duncan test, indicating a significant difference at a P <0.05 level.
TABLE 1 sodium hydrosulfide treatment of biomass, chlorophyll, soluble proteins of American Suaeda salsa
As can be seen from Table 1, the biomass, chlorophyll content and soluble protein content of the American Susbane lettuce treated by sodium hydrosulfide solutions with different concentrations (0, 0.01, 0.05, 0.1, 0.2, 0.4 and 0.8 mmol/L) are respectively counted, and the biomass, chlorophyll content and soluble protein content of the American Susbane lettuce treated by the sodium hydrosulfide solution with the concentration of 0.01 ~ 0.2.2 mmol/L are all improved to a certain extent compared with the control group, but the biomass, chlorophyll content and soluble protein content of the American Susbane lettuce treated by the sodium hydrosulfide solution with the concentration of 0.1 mmol/L have the best treatment effect, and when the treatment concentration of the sodium hydrosulfide is 0.1 mmol/L, the biomass of each lettuce is averagely increased by 3.74g, the chlorophyll content of 0.16 mg/g and the soluble protein content of the lettuce is averagely increased by 0.47 g/100 g compared with the control group.
Example 2
The application of sodium hydrosulfide as the donor of hydrogen sulfide gas in improving the yield of Chinese cabbage and improving the nutritional quality.
In this case, the experiment of using chinese cabbage to make hydrogen sulfide donor sodium hydrosulfide to increase crop yield and improve nutritional quality was carried out in the same manner as in example 1. Weighing fresh weight of picked Chinese cabbage with electronic balance, collecting leaf of Chinese cabbage, and determining chlorophyll and soluble protein content. The experiment is repeated three times, and the content of chlorophyll and protein in the sample, which are leaves at the same position of the Chinese cabbage, is measured every time. The results are shown in table 2 and are expressed as "mean ± standard deviation", and the difference between the treatment and control groups was analyzed using the Duncan test, indicating a significant difference at a P <0.05 level.
TABLE 2 sodium hydrosulfide treatment of biomass, chlorophyll, soluble proteins of chinese cabbage
As can be seen from Table 2, the biomass, the chlorophyll content and the soluble protein content of the Chinese cabbage after being treated with sodium hydrosulfide solutions of different concentrations (0, 0.01, 0.05, 0.1, 0.2, 0.4 and 0.8 mmol/L) are respectively counted, and the biomass, the chlorophyll content and the soluble protein content of the Chinese cabbage after being treated with the sodium hydrosulfide solution of 0.01 ~ 0.2.2 mmol/L concentration are all improved to a certain extent compared with the control group, but the treatment effect of the sodium hydrosulfide solution of 0.1 mmol/L concentration is the best, when the treatment concentration of the sodium hydrosulfide is 0.1 mmol/L, the biomass of each Chinese cabbage is increased by 11.15g on average, the chlorophyll content is increased by 0.51 mg/g on average, and the soluble protein content is increased by 0.21 g/100 g on average compared with the control group.
Example 3
The application of sodium hydrosulfide as hydrogen sulfide donor in raising yield of Suzhou rape and improving nutritive quality.
In the embodiment of the invention, Suzhou green rape is adopted to carry out an experiment of increasing crop yield and improving nutritional quality by using hydrogen sulfide donor sodium hydrosulfide, and the specific implementation steps are the same as those in the embodiment 1. Weighing fresh weight of the picked Suzhou green rape by using an electronic balance, collecting rape leaves and determining the content of chlorophyll and soluble protein. The experiment is repeated three times, and the content of chlorophyll and protein in the sample is determined by the content of the leaf at the same position of Suzhou green rape. The results are shown in table 3 and are expressed as "mean ± standard deviation", and the difference between the treatment and control groups was analyzed using the Duncan test, indicating a significant difference at a P <0.05 level.
TABLE 3 sodium hydrosulfide treatment of biomass, chlorophyll, soluble proteins of Suzhou rape
As can be seen from Table 3, the biomass, chlorophyll content and soluble protein content of Suzhou green rape treated by sodium hydrosulfide solutions with different concentrations (0, 0.01, 0.05, 0.1, 0.2, 0.4 and 0.8 mmol/L) are respectively counted, and the biomass, chlorophyll content and soluble protein content of Suzhou green rape treated by the sodium hydrosulfide solution with the concentration of 0.01 ~ 0.2.2 mmol/L are all improved to a certain extent compared with the control group, but the treatment effect of the sodium hydrosulfide solution with the concentration of 0.1 mmol/L is the best, when the treatment concentration of the sodium hydrosulfide is 0.1 mmol/L, the biomass of each rape is increased by 5.28g on average, the chlorophyll content is increased by 0.39mg/g on average, and the soluble protein content is increased by 1.40 g/100 g on average compared with the control group.
Example 4
The application of sodium hydrosulfide as hydrogen sulfide donor in raising the yield of Chinese green Chinese cabbage and improving its nutritive quality.
In this case, the experiment of using "four seasons" of Chinese little greens to carry out the experiment of using sodium hydrosulfide as hydrogen sulfide donor to increase the crop yield and improve the nutritional quality is carried out in the same way as in example 1. Weighing fresh weight of picked four-season herba Solani Lyrati with electronic balance, collecting herba Solani Lyrati leaf, and determining chlorophyll and soluble protein content. The experiment is repeated three times, and the content of chlorophyll and protein in the sample, which are leaves at the same position of the four-season Chinese little greens, is measured each time. The results are shown in table 4 and are expressed as "mean ± standard deviation", and the difference between the treatment and control groups was analyzed using the Duncan test, indicating a significant difference at a P <0.05 level.
TABLE 4 sodium hydrosulfide treatment of biomass, chlorophyll, soluble protein of Siji swamp cabbage
As can be seen from Table 4, the biomass, the chlorophyll content and the soluble protein content of the four-season Chinese cabbage after being treated by sodium hydrosulfide solutions with different concentrations (0, 0.01, 0.05, 0.1, 0.2, 0.4 and 0.8 mmol/L) are respectively counted, and the biomass, the chlorophyll content and the soluble protein content of the four-season Chinese cabbage after being treated by the sodium hydrosulfide solution with the concentration of 0.01 ~ 0.2.2 mmol/L are all improved to a certain extent compared with a control group, but the treatment effect of the sodium hydrosulfide solution with the concentration of 0.1 mmol/L is the best, when the treatment concentration of the sodium hydrosulfide is 0.1 mmol/L, the biomass of each Chinese cabbage is increased by 6.73g on average, the chlorophyll content of 0.48mg/g on average, and the soluble protein content of 1.01g/100 g on average compared with the control group.
Example 5
Application of sodium hydrosulfide as hydrogen sulfide donor in increasing yield of Sukang No. 3 mung bean and improving nutritional quality.
In the period of growing true leaves of crops at 3 ~ 4 rd, performing root irrigation treatment on the crops for 1 ~ 3 times by using a sodium hydrosulfide solution with the concentration of 0 ~ 0.2.2 mmol/L at an interval of 2 ~ 5 days, and in the period of flowering and pod bearing, spraying a sodium hydrosulfide and tween mixed solution with the concentration of 0.01 ~ 0.2.2 mmol/L on the leaves of the crops for 3 ~ 6 times continuously at an interval of 7 ~ 10 days.
The spraying sodium hydrosulfide and tween mixed solution is prepared by uniformly mixing 0.01 ~ 0.2.2 mmol/L sodium hydrosulfide solution and tween-80 according to the volume of 500 ~ 1000: 1.
Experiment design: the test is carried out in an open-air test field, the test is designed and processed for 3 times, the test is repeated for 3 times, and random block group field arrangement is adopted, wherein the area of each cell is 9m2 (3 m multiplied by 3 m), the plant spacing is 30 cm, the row spacing is 60 cm, the behavior is 1 cell, and the first row spacing is 30 cm.
The method comprises the following specific steps:
1. selecting open-air large fields in the experimental land, selecting full, uniform and consistent pea seeds for sowing, ploughing and preparing soil before sowing, adopting an artificial dibbling mode and local conventional cultivation and management, and fixing seedlings for 1 time in the whole growth period;
2. in the period of growing true leaves of 3 rd 3 ~ 4 rd pair of crops, performing root irrigation treatment on the crops for 1 ~ 3 times by using sodium hydrosulfide solution with the concentration of 0 ~ 0.2.2 mmol/L, wherein each time interval is 2 ~ 5 days;
3. spraying the mixed solution of sodium hydrosulfide and tween with the concentration of 0 ~ 0.2.2 mmol/L on the leaf surfaces of the crops in the flowering and pod bearing period of the crops for 3 ~ 6 times continuously with the interval of 7 ~ 10 days each time;
4. when the seeds are mature, collecting 3 rows in the middle of each cell, randomly taking 10 plants from the middle of each cell for seed test, and removing 0.5m from two ends, wherein the seed test items comprise the number of pods of each plant, the number of grains of each plant, the yield of each plant and the weight of hundreds of grains; and selecting mature and plump seeds to measure the content of soluble sugar and soluble protein, and measuring the content of trace elements by using a mung bean powder sample ground by a grinding machine.
In the embodiment, the Sukang No. 3 mung beans are used for carrying out an experiment of increasing the crop yield and improving the nutritional quality by using sodium hydrosulfide as a hydrogen sulfide donor, when seeds are mature, 10 plants are randomly selected from the middle of each cell for seed test, and the number of pods of each plant, the number of grains of each plant, the yield of each plant and the weight of hundreds of grains are counted; selecting mature and plump seeds and measuring the content of soluble sugar and soluble protein; the content of trace elements in the beans is determined by using the ground mung bean powder sample. Data were observed and recorded, and the results are shown in table 5:
TABLE 5 Single-plant grain number, Single-plant yield, hundred-grain weight of sodium hydrosulfide treated Sukang No. 3 mung beans
As can be seen from table 5, four yield indexes of the number of pods per plant, the number of grains per plant, the yield per plant and the weight of grains of the sukang 3 mung beans treated with sodium hydrosulfide solutions of different concentrations (0, 0.1 and 0.2 mmol/L) were respectively counted, and it was found that the number of pods per plant, the number of grains per plant, the yield per plant and the weight of grains of the sukang 3 mung beans after being treated with the sodium hydrosulfide solution were all improved to a certain extent compared with the control group. When the treatment concentration of the sodium hydrosulfide is 0.1 mmol/L, the number of pods of a single plant, the number of grains of the single plant, the yield of the single plant and the weight of the hundred grains of the Sukang No. 3 mung bean achieve the best effect, compared with a control, the number of pods of the single plant is increased by 11.4 on average, the number of grains of the single plant is increased by 101.76 grains, the yield of the single plant is increased by 9.27 g, and the weight of the hundred grains is increased by 0.82 g.
Example 6
Use of sodium hydrosulfide as a donor of hydrogen sulfide gas for increasing yield of large pod 618 peas and improving nutritional quality.
In the 3 rd 3 ~ 4 th period of crop growth, sodium hydrosulfide solution with concentration of 0 ~ 0.8.8 mmol/L is used for carrying out root irrigation treatment for 1 ~ 3 times at intervals of 2 ~ 5 days for the true leaves, and sodium hydrosulfide Tween mixed solution with concentration of 0.01 ~ 0.8.8 mmol/L is used for carrying out spraying treatment on the leaves of the crops at the flowering and pod bearing period, and the spraying is continuously carried out for 3 ~ 6 times at intervals of 7 ~ 10 days for each time;
the spraying sodium hydrosulfide and tween mixed solution is prepared by uniformly mixing 0.01 ~ 0.8.8 mmol/L sodium hydrosulfide solution and tween-80 according to the volume ratio of 500 ~ 1000: 1.
Experiment design: the test is carried out in an open-air test field, the test is designed and processed for 6 times without repetition, and the field arrangement of random block, the plant spacing of 30 cm, the row spacing of 60 cm and the first row spacing of 30 cm are adopted.
The method comprises the following specific steps:
1. and selecting open-air large fields in the experimental land, selecting full, uniform and consistent pea seeds for sowing, ploughing and preparing soil before sowing, and adopting a manual dibbling mode and local conventional cultivation management.
2. In the period of growing 3 ~ 4 pairs of true leaves, performing root irrigation treatment on the crops with sodium hydrosulfide solution with the concentration of 0 ~ 0.8.8 mmol/L for 1 ~ 3 times at intervals of 2 ~ 5 days;
3. spraying the mixed solution of sodium hydrosulfide and tween with the concentration of 0.01 ~ 0.8.8 mmol/L on the leaf surfaces of the crops in the flowering and pod bearing period of the crops for 3 ~ 6 times at intervals of 7 ~ 10 days;
4. when the seeds are mature, collecting 3 rows in the middle of each cell, randomly taking 10 plants from the middle of each cell for seed test, and removing 0.5m from two ends, wherein the seed test items comprise the grain number of each plant, the yield of each plant and the weight of each hundred grains; and selecting mature and plump seeds to measure the content of soluble sugar and soluble protein, and measuring the content of trace elements by using a pea powder sample ground by a grinding machine.
In the embodiment, a large pod 618 pea is adopted to carry out an experiment of increasing the crop yield and improving the nutritional quality by using hydrogen sulfide donor sodium hydrosulfide, when seeds are mature, 10 plants are randomly selected from the middle of each cell to test the seeds, and the grain number, the yield and the hundred grain weight of each plant are counted; selecting mature and plump seeds and measuring the content of soluble sugar and soluble protein; the content of trace elements in the beans was determined using a ground pea powder sample. Data were observed and recorded, and the results are shown in tables 6, 7, and 8:
TABLE 6 Single-plant grain number, Single-plant yield, hundred-grain weight of sodium hydrosulfide treated big pod 618 peas
As can be seen from Table 6, four yield indexes of the single-plant grain number, the single-plant yield and the hundred grain weight of the big pod 618 peas treated by the sodium hydrosulfide solutions with different concentrations (0, 0.1 and 0.2 mmol/L) are respectively counted, and the single-plant grain number, the single-plant yield and the hundred grain weight of the big pod 618 peas treated by the sodium hydrosulfide solution with the concentration of 0.05 ~ 0.2.2 mmol/L are all improved to a certain extent compared with a control group.
TABLE 7 soluble protein, soluble sugar content of sodium hydrosulfide treated big pod 618 peas
| Treatment of | Soluble protein content (%) | Soluble sugar content (%) |
| Con-Con | 20.82±0.02c | 4.47±0.01d |
| 0.05-Con | 20.49±0.20b | 5.01±0.11c |
| 0.1-Con | 21.51±0.11d | 5.20±0.09bc |
| 0.2-Con | 21.83±0.09a | 5.27±0.04d |
| 0.4-Con | 19.18±0.06e | 5.11±0.05a |
| 0.8-Con | 20.92±0.10c | 4.48±0.09ab |
As can be seen from Table 7, the content of soluble protein and soluble sugar in the fruits of peas in big pod 618 treated with sodium hydrosulfide solutions of different concentrations (0, 0.05, 0.1, 0.2, 0.4, 0.8 mmol/L) is respectively counted, and the content of soluble protein and soluble sugar in the fruits of peas in big pod 618 treated with the sodium hydrosulfide solution of 0.05 ~ 0.2.2 mmol/L is improved to a certain extent compared with the content in a control group.
TABLE 8 microelement content of sodium hydrosulfide treated big pod 618 peas
| Treatment of | P(mg/g) | K(mg/g) | Zn(mg/kg) | Mg(mg/g) |
| Con-Con | 3.99±0.16 | 12.50±0.28 | 53.61±0.63 | 1.67±0.0462 |
| 0.05-Con | 4.13±0.06 | 12.20±0.06 | 53.81±1.44 | 1.59±0.0058 |
| 0.1-Con | 4.30±0.09 | 12.97±0.13 | 51.08±0.27 | 1.59±0.0153 |
| 0.2-Con | 4.47±0.04 | 12.54±0.06 | 54.64±0.74 | 1.62±0.0404 |
| 0.4-Con | 3.55±0.03 | 11.64±0.26 | 51.48±0.38 | 1.50±0.0300 |
| 0.8-Con | 4.20±0.09 | 12.28±0.26 | 57.68±0.68 | 1.57±0.0057 |
As can be seen from table 8, the content of trace elements in the big pod 618 pea powder samples treated with different concentrations of sodium hydrosulfide solutions (0, 0.05, 0.1, 0.2, 0.4, 0.8 mmol/L) was counted, and it was found that the content of trace elements in the big pod 618 pea treated with the sodium hydrosulfide solution was increased to different degrees compared with the control group.
Example 7
The application of sodium hydrosulfide as a hydrogen sulfide gas donor in improving the yield of RL109 peas and improving the nutritional quality.
In this example, RL109 peas were used to perform experiments on sodium hydrosulfide as a hydrogen sulfide donor to improve crop yield and improve nutritional quality, and the specific implementation steps were the same as those in example 6. When the seeds are mature, randomly taking 10 plants from the middle part of each cell for seed test, and counting the grain number of each plant, the yield of each plant and the weight of each hundred grains; selecting mature and plump seeds and measuring the content of soluble sugar and soluble protein; the content of trace elements in the beans was determined using a ground pea powder sample. Data were observed and recorded, and the results are shown in tables 9, 10, and 11:
TABLE 9 number of grains per plant, yield per plant, and weight per grain of sodium hydrosulfide treated RL109 peas
As can be seen from Table 9, four yield indexes of the number of grains per plant, the yield per plant and the weight per grain of the RL109 peas treated by sodium hydrosulfide solutions with different concentrations (0, 0.1 and 0.2 mmol/L) are respectively counted, and the number of grains per plant, the yield per plant and the weight per grain of the RL109 peas treated by the sodium hydrosulfide solution with the concentration of 0.05 ~ 0.2.2 mmol/L are all improved to a certain extent compared with a control group.
TABLE 10 soluble protein, soluble sugar content of sodium hydrosulfide treated RL109 peas
| Treatment of | Soluble protein content (%) | Soluble sugar content (%) |
| Con-Con | 22.61±0.02de | 5.01±0.08a |
| 0.05-Con | 22.67±0.01b | 4.91±0.07d |
| 0.1-Con | 23.72±0.08e | 5.24±0.06b |
| 0.2-Con | 24.54±0.09d | 5.35±0.09b |
| 0.4-Con | 22.77±0.13a | 4.39±0.06e |
| 0.8-Con | 22.98±0.07c | 4.83±0.05c |
As can be seen from Table 10, the content of soluble protein and soluble sugar in RL109 pea powder samples treated with different concentrations of sodium hydrosulfide solution (0, 0.05, 0.1, 0.2, 0.4, 0.8 mmol/L) was counted, and it was found that the content of soluble protein and soluble sugar in RL109 peas treated with 0.05 ~ 0.2.2 mmol/L sodium hydrosulfide solution was improved to a certain extent compared with the control group.
TABLE 11 microelement content of sodium hydrosulfide treated RL109 peas
| Treatment of | P(mg/g) | K(mg/g) | Zn(mg/kg) | Mg(mg/g) |
| Con-Con | 4.11±0.11 | 9.73±0.29 | 55.40±1.28 | 1.44±0.0153 |
| 0.05-Con | 4.18±0.07 | 9.64±0.09 | 50.75±0.41 | 1.47±0.0058 |
| 0.1-Con | 4.04±0.11 | 9.87±0.07 | 61.57±0.37 | 1.44±0.0100 |
| 0.2-Con | 4.26±0.14 | 9.56±0.12 | 55.76±1.39 | 1.47±0.0212 |
| 0.4-Con | 5.01±0.08 | 10.38±0.31 | 56.48±0.81 | 1.53±0.0603 |
| 0.8-Con | 4.03±0.10 | 10.16±0.09 | 53.21±1.16 | 1.51±0.0354 |
As can be seen from table 11, the content of trace elements in the RL109 pea powder samples treated with different concentrations of sodium hydrosulfide solutions (0, 0.05, 0.1, 0.2, 0.4, 0.8 mmol/L) was counted, and it was found that the content of trace elements in the RL109 peas treated with the sodium hydrosulfide solution was improved to different degrees compared with the control group.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It should be understood by those skilled in the art that the above embodiments do not limit the present invention in any way, and all technical solutions obtained by using equivalent alternatives or equivalent variations fall within the scope of the present invention.
Claims (8)
1. Use of a hydrogen sulfide donor sodium hydrosulfide for increasing crop yield and improving nutritional quality.
2. Use of a hydrogen sulfide donor sodium hydrosulfide for increasing the yield and improving the nutritional quality of crops according to claim 1, characterized in that said crops comprise leaf vegetables and legume crops.
3. The use of a hydrogen sulfide donor sodium hydrosulfide for improving the yield and the nutritional quality of crops as claimed in claim 2, characterized in that the seeds of leaf vegetables are subjected to germination acceleration culture, and when the crops grow to the heart of two leaves, the root soaking treatment is started by using a sodium hydrosulfide solution with the concentration of 0 ~ 0.8.8 mmol/L.
4. The use of a hydrogen sulfide donor, sodium hydrosulfide for improving crop yield and improving nutritional quality as claimed in claim 3, characterized in that the specific method of germination cultivation is:
a. accelerating germination of seeds of leaf vegetables at the temperature of 22 ~ 25 ℃;
b. seedlings after growing one leaf and one heart are transferred to Hoagland nutrient solution for growth under the conditions that the light/dark period is 16/8 h and the temperature is 20 ~ 26 ℃.
5. Use of a hydrogen sulfide donor sodium hydrosulfide for increasing the yield of crops and improving the nutritional quality according to claims 3 or 4, characterized in that the concentration of the sodium hydrosulfide solution is 0.01 ~ 0.2.2 mmol/L.
6. The use of the hydrogen sulfide donor sodium hydrosulfide for improving the yield and the nutritional quality of crops as claimed in claim 2, characterized in that seeds of legume crops are planted and cultured, when the crops grow to the seedling stage, the seeds are subjected to root irrigation treatment by using a sodium hydrosulfide solution with the concentration of 0 ~ 0.8.8 mmol/L, and when the crops grow to the flowering and pod stage, the leaves of the crops are sprayed by using a sodium hydrosulfide and tween mixed solution.
7. Use of a hydrogen sulfide donor sodium hydrosulfide for improving crop yield and improving nutritional quality according to claim 6, characterized in that the concentration of said sodium hydrosulfide solution is 0.05 ~ 0.2.2 mmol/L.
8. The use of sodium hydrosulfide as hydrogen sulfide donor in increasing crop yield and improving nutritional quality as claimed in claim 6, wherein said mixed solution of sodium hydrosulfide and tween is prepared by mixing 0.01 ~ 0.8.8 mmol/L sodium hydrosulfide solution and tween-80.
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