CN101473720B - Method for promoting morphological after-ripening of ginseng embryo using gibberellic acid mixed with sulfourea - Google Patents
Method for promoting morphological after-ripening of ginseng embryo using gibberellic acid mixed with sulfourea Download PDFInfo
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- CN101473720B CN101473720B CN2009100664603A CN200910066460A CN101473720B CN 101473720 B CN101473720 B CN 101473720B CN 2009100664603 A CN2009100664603 A CN 2009100664603A CN 200910066460 A CN200910066460 A CN 200910066460A CN 101473720 B CN101473720 B CN 101473720B
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Abstract
The invention relates to a method for promoting the morphological post maturation of ginseng embryo by the blending of gibberellic acid and thiourea, belonging to the agriculture technical field. The method solves the problems of long morphological post maturation time and low rate of emergence in the process of morphological post maturation treatment of the ginseng embryo. The method comprises the following steps: by percentage by weight, 0.002-0.01percent of gibberellic acid, 0.2-0.5 percent of thiourea and the balance of water are mixed to form a mixed water solution; and prior to artificial germination, ginseng seeds are soaked for 15-36 hours, and then the artificial germination is carried out. Applied to the treatment of the morphological post maturation of ginseng embryo, the method can shorten the morphological post maturation time and increase the split rate, the embryo rate and the emergence rate of the ginseng seeds.
Description
Technical field:
The invention belongs to agricultural technology field, relate to a kind of gibberellic acid and thiocarbamide and use the method that promotes morphological post maturation of ginseng embryo with.
Background technology:
Ginseng seeds belongs to the incomplete type of embryonic development.Behind the seed collection, other each several part is organized and all or has substantially been reached the stage of ripeness, and embryo is not also grown fully, only is made of minority embryo archaeocyte.The ginseng seeds embryo of newly gathering is very little, is about 0.32～0.43mm, wide about 0.25mm, and endosperm is about 5～6mm, wide about 4mm, and the endosperm area is 180～300 times of embryo area.Therefore, must be after ginseng seeds is gathered through after-ripening process ability germination and emergence, ginseng seeds after-ripening process is divided into the after-ripening of embryo form and two stages of physiology after-ripening, and do not finish early stage, later stage then can not pass through, so whether the embryo form after-ripening stage finishes very important smoothly.The form after-ripening is embryo slow Growth and Differentiation and type-approval process in endosperm, if the ginseng seeds autumn sowing after will gathering in the field, needed 18～22 months just can finish the after-ripening process under field conditions (factors).If under suitable conditions such as temperature, moisture and oxygen, carry out artificial germination, need 3～4 months the time could the completion morphology after-ripening, but emergence rate be still lower.Therefore, take certain measure, promote the differentiation and the growth of embryo, help morphological post maturation of ginseng embryo, it is all higher to be embodied in seed breach rate, embryo rate and emergence rate.
Retrieval shows, at present but still find no gibberellic acid and thiocarbamide are mixed with mixed aqueous solution, is used to promote the patented technology of morphological post maturation of ginseng embryo aspect.The present invention is mixed with mixed aqueous solution with gibberellic acid and thiocarbamide, at vernalization pre-treatment ginseng seeds, solved the long and low problem of emergence rate of the form ripening time that in the morphological post maturation of ginseng embryo processing procedure, exists, reached and promoted morphological post maturation of ginseng embryo, the purpose of raising seed breach rate, embryo rate and emergence rate.
Summary of the invention:
The object of the invention provides a kind of gibberellic acid and thiocarbamide is used the method that promotes morphological post maturation of ginseng embryo with, is used for solving the problems referred to above that ginseng seeds form after-ripening processing procedure exists.
Gibberellic acid of the present invention and thiocarbamide are used the method that promotes morphological post maturation of ginseng embryo with, its technical characterictic is, by weight percentage 0.002%～0.01% gibberellic acid, 0.2%～0.5% thiocarbamide and water (surplus) are mixed with mixed aqueous solution, before artificial germination, soak ginseng seeds 15～36 hours, and then carry out artificial germination.
Gibberellic acid of the present invention and thiocarbamide are used the optimization method that promotes morphological post maturation of ginseng embryo with, its technical characterictic is, by weight percentage 0.004%～0.008% gibberellic acid, 0.3%～0.4% thiocarbamide and water (surplus) are mixed with mixed aqueous solution, before artificial germination, soak ginseng seeds 20～24 hours, and then carry out artificial germination.
Raw materials used be in the agricultural production allows to use among the present invention, and cost is low, and is easy to use.
The effect of each component among the present invention:
1, separated so far and the gibberellin (GA) that identifies has kind more than 100, it is ubiquitous a kind of endogenous hormones in the plant corpus, extensively be present in the organs such as root, stem, leaf, flower, fruit and seed of higher plant, but content seldom.The gibberellin that uses among the present invention is gibberellic acid (GA
3), be 85% pulvis, physiologically active is strong, and purposes is wide; Molecular formula: C
19H
22O
6, relative molecular mass: 346.0; Be insoluble in water, be soluble in organic solvents such as ethanol.Its major physiological effect is: break the dormancy of seed, stem tuber and piece root, impel sprouting; The stimulating plant cell elongation is grown tall plant and blade increases; Stimulate fruit growth and improve ripening rate etc.
2, thiocarbamide is a kind of thio urea that weak hormonal action is arranged, and pure product are white crystals, is dissolved in cold water and alcohols.The thiocarbamide that uses among the present invention is 98.0% crystalline solid, molecular formula: CH
4N
2S, relative molecular mass: 76.12.Its major physiological effect is: can suppress the activity of plant corpus hydrogen peroxide enzyme, and catalase can hinder the sprouting of seed usually, thereby handle the effect that some seed has breaking dormancy, promotes sprouting with thiocarbamide.
Test effect of the present invention:
Test is carried out in the Jilin Agriculture University laboratory.
1 materials and methods
1) test material
Gibberellic acid is the pulvis of purity 85.0%, and thiocarbamide is the crystalline solid of purity 98.0%.Germinating box is made by the 3cm solid wood, and vernalization matrix is formulated by 3: 1 by humus soil and river sand.Test is the dry seeds of gathering last one year with ginseng seeds, and kind is " Da-maya ".
3) test is handled and method
Corresponding seed quality criteria meets GB6941-86 ginseng seeds standard, thousand kernel weight＞30g, vitality＞98% (red tetrazolium decoration method mensuration).The polyoxin aqueous solution soaking seed of the preceding usefulness of test 150mg/kg 24 hours carries out disinfection.
Test is handled: 4 processing are established in test: a. clear water (CK) soaked ginseng seeds 24 hours; B.0.006% gibberellic acid aqueous solution soaking seed 24 hours; C.0.35% thiourea solution soaked seed 24 hours; D.0.006% the mixed aqueous solution of gibberellic acid+0.35% thiocarbamide soaked seed 24 hours.Each handles 1000 seeds, 3 repetitions.
Test method: before the vernalization gibberellic acid and thiocarbamide are mixed with the aqueous solution by processing requirements.Before the preparation, with micro-liquor or alcohol dissolving, thiocarbamide dissolves with low amounts of water gibberellic acid, and then preparation respectively earlier; Handling a is that clear water soaks, and each is handled the seed soak time and is 24 hours.
Beginning vernalization on June 25th, 2007.Earlier humus soil and river sand (sieving) were mixed with mixed-matrix by 3: 1, spread the thick matrix of 5cm, reinstall the mixture (matrix is mixed by 3: 1 with seed) of matrix and seed, the thick matrix of upper strata covering 10cm in germinating box (groove) bottom.In earlier stage (before the seed breach) keeps 18～20 ℃ of substrate temperature, and the later stage (behind the seed breach) keeps 13～15 ℃ of substrate temperature; In earlier stage (before the seed breach) keeps soil humidity 19%～20%, and the later stage (behind the seed breach) keeps soil humidity 14%～15%; The seed breach fell to plant once in preceding 15 days, fell to plant once in 7 days behind the breach.Other requirement by artificial germination manages, and seed breach rate and embryo rate were measured in vernalization on the 90th day.On October 15th, 2007 is with pressing an eye device by artificial seeding, one in every cave, 4cm * 4cm program request, other field management requirements by genseng production is carried out, on June 20th, 2008 the mensuration emergence rate.
2, result of the test
Result of the test shows (table 1), mixed aqueous solution with 0.006% gibberellic acid+0.35% thiocarbamide soaked ginseng seeds 24 hours, ginseng seeds breach rate, embryo rate and emergence rate are respectively 96.2%, 92.5% and 90.5%, and effect significantly is better than clear water (CK), uses 0.006% gibberellin (GA separately
3) and the processing of 0.35% thiocarbamide, reach extremely significantly standard.
Table 1 different disposal is to the influence of morphological post maturation of ginseng embryo effect
Annotate: compare with control group,
*P＜0.01
Illustrate:
1) breach rate: breach seed/seed total amount * 100% (after the ginseng embryo Growth and Differentiation, idiosome is long-pending to be increased gradually, and the turgescence of embryo forces pericarp to be called " breach " along the phenomenon in conjunction with the trace cracking, and the breach rate is the ratio of breach seed and seed total amount);
2) embryo rate: embryo length/endosperm long * 100%;
3) emergence rate: the number/seeding quantity of emerging * 100%.
3, conclusion
Result of the test of the present invention shows, gibberellic acid and thiocarbamide are mixed with mixed aqueous solution, and at vernalization pre-treatment ginseng seeds, after-ripening has appreciable impact to the embryo form, and breach rate, embryo rate and the emergence rate of seed are significantly increased.The invention solves the long and low problem of emergence rate of the form ripening time that in the morphological post maturation of ginseng embryo processing procedure, exists, reached and promoted morphological post maturation of ginseng embryo, the purpose of raising seed breach rate, embryo rate and emergence rate.
Embodiment:
By weight percentage, get 0.002%～0.01% gibberellic acid (GA
3, purity is 85.0% pulvis), with micro-liquor or alcohol dissolving; Get 0.2%～0.5% thiocarbamide (purity is 98.0% crystalline solid),, successively inject gauge water again, be mixed with mixed aqueous solution, before artificial germination, soaked ginseng seeds 15～36 hours, and then carry out artificial germination with the low amounts of water dissolving.
Embodiment: by weight percentage, get 0.006% gibberellic acid, 0.35% thiocarbamide, water surplus is made mixed aqueous solution, soaks ginseng seeds 24 hours, carries out artificial germination then.
For example, get gibberellic acid 60mg, with micro-liquor or alcohol dissolving; Get thiocarbamide 3500mg, dissolve with low amounts of water.And then above-mentioned 2 kinds of materials are successively injected the 1.0Kg clear water, and be mixed with mixed aqueous solution, before artificial germination, soaked ginseng seeds 24 hours.Other ginseng seeds vernalization condition is handled by the requirement of artificial germination, can reach purpose of the present invention.
Claims (1)
1. gibberellic acid and thiocarbamide are used the method that promotes morphological post maturation of ginseng embryo with, its technical characterictic is: get gibberellic acid 60mg, with micro-liquor or alcohol dissolving, get thiocarbamide 3500mg, dissolve with low amounts of water, and then above-mentioned 2 kinds of materials are successively injected the 1.0Kg clear water, and be mixed with mixed aqueous solution, before artificial germination, soaked ginseng seeds 24 hours.
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| CN2009100664603A CN101473720B (en) | 2009-01-20 | 2009-01-20 | Method for promoting morphological after-ripening of ginseng embryo using gibberellic acid mixed with sulfourea |
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| CN2009100664603A CN101473720B (en) | 2009-01-20 | 2009-01-20 | Method for promoting morphological after-ripening of ginseng embryo using gibberellic acid mixed with sulfourea |
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| CN101473720A CN101473720A (en) | 2009-07-08 |
| CN101473720B true CN101473720B (en) | 2011-06-22 |
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| CN106212020A (en) * | 2016-08-16 | 2016-12-14 | 集安市小青沟人参种植专业合作社 | A kind of implantation methods of high yield Panax Ginseng |
| CN108064885A (en) * | 2017-12-14 | 2018-05-25 | 吉林师范大学 | A kind of microbial bacterial agent for improving ginseng quality and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1016837B (en) * | 1988-11-24 | 1992-06-03 | 中国医学科学院药用植物资源开发研究所 | Accelerating germination technology for seed of american ginseng |
| CN101228828A (en) * | 2008-02-20 | 2008-07-30 | 延边长白山红景天种植有限公司 | Culture technic of introducing rhodiola rosea to low elevation |
-
2009
- 2009-01-20 CN CN2009100664603A patent/CN101473720B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1016837B (en) * | 1988-11-24 | 1992-06-03 | 中国医学科学院药用植物资源开发研究所 | Accelerating germination technology for seed of american ginseng |
| CN101228828A (en) * | 2008-02-20 | 2008-07-30 | 延边长白山红景天种植有限公司 | Culture technic of introducing rhodiola rosea to low elevation |
Non-Patent Citations (4)
| Title |
|---|
| 刘继生等.外源赤霉素对东北刺人参种子萌发的影响.《林业科技》.2005,第30卷(第2期),45-47. * |
| 王艳等.赤霉素、硫脲对陈化藿香种子活力的影响.《种子》.2006,第25卷(第3期),13-14、18. * |
| 董玉明等.西洋参种子催芽技术研究.《中国药科大学学报》.1996,第27卷(第8期),511-512. * |
| 郭耀忠等.GA对人参种子出苗率影响的试验.《吉林林业科技》.1989,(第2期),51-52. * |
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