CN106459950A - 通过交联的高负载酶固定化 - Google Patents

通过交联的高负载酶固定化 Download PDF

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Publication number
CN106459950A
CN106459950A CN201580017609.2A CN201580017609A CN106459950A CN 106459950 A CN106459950 A CN 106459950A CN 201580017609 A CN201580017609 A CN 201580017609A CN 106459950 A CN106459950 A CN 106459950A
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CN
China
Prior art keywords
molecule
cross
enzyme
present
linking
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CN201580017609.2A
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English (en)
Chinese (zh)
Inventor
C.霍恩
S.加文达
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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Publication of CN106459950A publication Critical patent/CN106459950A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/06—Enzymes or microbial cells immobilised on or in an organic carrier attached to the carrier via a bridging agent
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/089—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01047—Glucose 1-dehydrogenase (1.1.1.47)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/902—Oxidoreductases (1.)
    • G01N2333/904—Oxidoreductases (1.) acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Emergency Medicine (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
CN201580017609.2A 2014-03-31 2015-03-27 通过交联的高负载酶固定化 Pending CN106459950A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP14162678.8A EP2927319A1 (en) 2014-03-31 2014-03-31 High load enzyme immobilization by crosslinking
EP14162678.8 2014-03-31
PCT/EP2015/056732 WO2015150263A1 (en) 2014-03-31 2015-03-27 High load enzyme immobilisation by crosslinking

Publications (1)

Publication Number Publication Date
CN106459950A true CN106459950A (zh) 2017-02-22

Family

ID=50396979

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201580017609.2A Pending CN106459950A (zh) 2014-03-31 2015-03-27 通过交联的高负载酶固定化

Country Status (7)

Country Link
US (1) US10400233B2 (ko)
EP (2) EP2927319A1 (ko)
JP (1) JP2017512478A (ko)
KR (1) KR20160138085A (ko)
CN (1) CN106459950A (ko)
CA (1) CA2943558A1 (ko)
WO (1) WO2015150263A1 (ko)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110133271A (zh) * 2018-02-09 2019-08-16 北京豪迈生物工程股份有限公司 一种将抗体或其抗原结合片段共价结合至颗粒表面的方法

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017151952A1 (en) 2016-03-04 2017-09-08 Abbott Diabetes Care Inc. Nad(p)-dependent responsive enzymes, electrodes and sensors, and methods for making and using the same

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CN101076601A (zh) * 2004-12-13 2007-11-21 拜尔保健有限公司 用于测量生物液体中的分析物的自我限定大小的组合物和检验设备
CN101303347A (zh) * 2007-04-20 2008-11-12 天津杏林书院医疗器械有限公司 生物传感器
KR20120130731A (ko) * 2011-05-23 2012-12-03 고려대학교 산학협력단 효소면역측정용 복합체 및 그 제조방법
CN102928479A (zh) * 2011-08-11 2013-02-13 北京怡成生物电子技术有限公司 电化学生物传感器试条及其制作方法
US9737250B2 (en) * 2013-03-15 2017-08-22 Dexcom, Inc. Membrane for continuous analyte sensors

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US5108564A (en) 1988-03-15 1992-04-28 Tall Oak Ventures Method and apparatus for amperometric diagnostic analysis
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EP0654079B1 (en) 1992-07-02 2000-03-22 Roche Diagnostics Corporation A reagent stabilized by bivalent metal salts
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US6054039A (en) 1997-08-18 2000-04-25 Shieh; Paul Determination of glycoprotein and glycosylated hemoglobin in blood
DE19753851A1 (de) 1997-12-04 1999-06-10 Roche Diagnostics Gmbh Vorrichtung zum kapillaren Flüssigkeitstransport
DE19753850A1 (de) 1997-12-04 1999-06-10 Roche Diagnostics Gmbh Probennahmevorrichtung
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JP4070050B2 (ja) 1998-07-24 2008-04-02 テルモ株式会社 血糖値測定方法及び装置
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PL205561B1 (pl) 2000-10-27 2010-05-31 Hoffmann La Roche Zmutowane białko zależnej od pirolochinolinochinonu rozpuszczalnej dehydrogenazy glukozowej, wyizolowany polinukleotyd kodujący to białko, wektor ekspresyjny, komórka gospodarza, sposób wytwarzania wariantów s-GDH, sposób wykrywania, oznaczania lub pomiaru glukozy i urządzenie do wykrywania lub pomiaru glukozy
EP1942195B1 (en) 2001-12-28 2010-09-22 ARKRAY, Inc. Colorimetric method and reagent used for the same
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110133271A (zh) * 2018-02-09 2019-08-16 北京豪迈生物工程股份有限公司 一种将抗体或其抗原结合片段共价结合至颗粒表面的方法

Also Published As

Publication number Publication date
EP2927319A1 (en) 2015-10-07
CA2943558A1 (en) 2015-10-08
US20170159039A1 (en) 2017-06-08
EP3126493A1 (en) 2017-02-08
JP2017512478A (ja) 2017-05-25
WO2015150263A1 (en) 2015-10-08
KR20160138085A (ko) 2016-12-02
EP3126493B1 (en) 2018-05-09
US10400233B2 (en) 2019-09-03

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