CN106459895B - 制备用于3d组织培养的细胞的方法 - Google Patents
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- CN106459895B CN106459895B CN201580020057.0A CN201580020057A CN106459895B CN 106459895 B CN106459895 B CN 106459895B CN 201580020057 A CN201580020057 A CN 201580020057A CN 106459895 B CN106459895 B CN 106459895B
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0062—General methods for three-dimensional culture
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0068—General culture methods using substrates
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/067—Hepatocytes
- C12N5/0671—Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
- G01N33/5067—Liver cells
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2513/00—3D culture
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
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- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
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- Genetics & Genomics (AREA)
- Cell Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1406716.9A GB201406716D0 (en) | 2014-04-15 | 2014-04-15 | Method of preparing cells for 3D tissue culture |
| GB1406716.9 | 2014-04-15 | ||
| PCT/EP2015/058174 WO2015158777A1 (en) | 2014-04-15 | 2015-04-15 | Method of preparing cells for 3d tissue culture |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106459895A CN106459895A (zh) | 2017-02-22 |
| CN106459895B true CN106459895B (zh) | 2021-06-01 |
Family
ID=50844988
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201580020057.0A Active CN106459895B (zh) | 2014-04-15 | 2015-04-15 | 制备用于3d组织培养的细胞的方法 |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US20170037364A1 (https=) |
| EP (1) | EP3132023B1 (https=) |
| JP (1) | JP6492106B2 (https=) |
| CN (1) | CN106459895B (https=) |
| AU (1) | AU2015248875B9 (https=) |
| CA (1) | CA2945548C (https=) |
| DK (1) | DK3132023T3 (https=) |
| GB (1) | GB201406716D0 (https=) |
| SG (1) | SG11201608463WA (https=) |
| WO (1) | WO2015158777A1 (https=) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6758026B2 (ja) * | 2015-04-17 | 2020-09-23 | 株式会社日立ハイテク | 成分分析装置、薬剤成分分析装置、成分分析方法及び薬剤成分分析方法 |
| EP3397753B1 (en) * | 2015-12-30 | 2021-10-27 | FUJIFILM Cellular Dynamics, Inc. | Microtissue formation using stem cell-derived human hepatocytes |
| GB201609663D0 (en) | 2016-06-02 | 2016-07-20 | Stemtek Therapeutics Sl | Methods for producing cancer stem cell spheroids |
| WO2018162623A1 (en) * | 2017-03-10 | 2018-09-13 | Merck Patent Gmbh | Infected cell cultures |
| CN109294971A (zh) * | 2018-09-29 | 2019-02-01 | 西安交通大学 | 一种用于药物筛选的颈动脉分叉三维细胞培养模型及其构建方法 |
| WO2020232436A1 (en) | 2019-05-16 | 2020-11-19 | Brown University | A human in vitro cardiotoxicity model |
| DE102019216886A1 (de) | 2019-10-31 | 2021-05-06 | Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. | Transfer zumindest eines partikels in einer flüssigkeit von einer abgabeeinheit zu einer empfangseinheit |
| CN111471641B (zh) * | 2020-02-03 | 2021-11-05 | 东华大学 | 多片层单元水凝胶包被的仿生毛细血管网的3d打印制法 |
| CN111150885A (zh) * | 2020-03-05 | 2020-05-15 | 陕西佰傲干细胞再生医学有限公司 | 软骨微囊及其制备方法和应用 |
| US12076437B2 (en) | 2021-07-12 | 2024-09-03 | Brown University | Proangiogenic protein cocktails delivered in custom biomaterials to revascularize ischemic tissue |
| CN121969735A (zh) | 2023-10-06 | 2026-05-01 | 银丝佛若有限公司 | 用于研究生物样本相互作用的装置 |
Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006032092A1 (en) * | 2004-09-24 | 2006-03-30 | Angioblast Systems, Inc. | Multipotential expanded mesenchymal precursor cell progeny (memp) and uses thereof |
| WO2007124023A2 (en) * | 2006-04-21 | 2007-11-01 | Wake Forest University Health Sciences | Ink-jet printing of tissues |
| WO2008051568A3 (en) * | 2006-10-23 | 2008-07-24 | Anthrogenesis Corp | Methods and compositions for treatment of bone defects with placental cell populations |
| WO2008100497A1 (en) * | 2007-02-12 | 2008-08-21 | Anthrogenesis Corporation | Hepatocytes and chondrocytes from adherent placental stem cells; and cd34+, cd45- placental stem cell-enriched cell populations |
| US20100124569A1 (en) * | 2008-11-19 | 2010-05-20 | Abbot Stewart | Amnion derived adherent cells |
| US20100129330A1 (en) * | 2002-04-03 | 2010-05-27 | Wilkison William O | Adipocytic differentiated adipose derived adult stem cells and uses thereof |
| WO2010060080A1 (en) * | 2008-11-24 | 2010-05-27 | Immunotrex Corporation | Three dimensional tissue generation |
| US20110229970A1 (en) * | 2010-03-05 | 2011-09-22 | Florida State University Research Foundation | Dual-chamber perfusion bioreactor for orthopedic tissue interfaces and methods of use |
| WO2011064669A3 (en) * | 2009-11-30 | 2011-12-15 | Pluristem Ltd. | Adherent cells from placenta and use of same in disease treatment |
| WO2011154552A1 (en) * | 2010-06-11 | 2011-12-15 | Cellartis Ab | 3-dimensional scaffolds for improved differentiation of pluripotent stem cells to hepatocytes |
| WO2012083023A1 (en) * | 2010-12-17 | 2012-06-21 | Anthrogenesis Corporation | Treatment of spinal cord injury and traumatic brain injury using amnion derived adherent cells |
| WO2013040078A2 (en) * | 2011-09-12 | 2013-03-21 | Organovo, Inc. | Engineered tissues for in vitro research uses, arrays thereof, and methods of making the same |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2545100B1 (fr) * | 1983-04-29 | 1985-12-20 | Inst Nat Sante Rech Med | Procede d'obtention de cultures d'hepatocytes humains, les cultures obtenues et leurs applications biologiques et biochimiques |
| WO2003055487A1 (en) * | 2002-01-04 | 2003-07-10 | The University Of Adelaide | METHOD OF CONTROLLING DAMAGE MEDIATED BY α, β- UNSATURATED ALDEHYDES |
| JP2004254674A (ja) * | 2003-02-25 | 2004-09-16 | Mutsumi Takagi | 動物細胞の分化誘導培養方法 |
| ES2609006T3 (es) * | 2009-10-16 | 2017-04-18 | Rutgers, The State University Of New Jersey | Procedimeinto de tratamiento de lesión crónica de tejido nervioso usando una estrategia de terapia celular |
-
2014
- 2014-04-15 GB GBGB1406716.9A patent/GB201406716D0/en not_active Ceased
-
2015
- 2015-04-15 US US15/303,455 patent/US20170037364A1/en not_active Abandoned
- 2015-04-15 CN CN201580020057.0A patent/CN106459895B/zh active Active
- 2015-04-15 SG SG11201608463WA patent/SG11201608463WA/en unknown
- 2015-04-15 WO PCT/EP2015/058174 patent/WO2015158777A1/en not_active Ceased
- 2015-04-15 EP EP15723435.2A patent/EP3132023B1/en active Active
- 2015-04-15 DK DK15723435.2T patent/DK3132023T3/da active
- 2015-04-15 JP JP2016562830A patent/JP6492106B2/ja active Active
- 2015-04-15 AU AU2015248875A patent/AU2015248875B9/en not_active Ceased
- 2015-04-15 CA CA2945548A patent/CA2945548C/en active Active
-
2023
- 2023-01-26 US US18/101,751 patent/US20230235277A1/en not_active Abandoned
Patent Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100129330A1 (en) * | 2002-04-03 | 2010-05-27 | Wilkison William O | Adipocytic differentiated adipose derived adult stem cells and uses thereof |
| WO2006032092A1 (en) * | 2004-09-24 | 2006-03-30 | Angioblast Systems, Inc. | Multipotential expanded mesenchymal precursor cell progeny (memp) and uses thereof |
| WO2007124023A2 (en) * | 2006-04-21 | 2007-11-01 | Wake Forest University Health Sciences | Ink-jet printing of tissues |
| WO2008051568A3 (en) * | 2006-10-23 | 2008-07-24 | Anthrogenesis Corp | Methods and compositions for treatment of bone defects with placental cell populations |
| WO2008100497A1 (en) * | 2007-02-12 | 2008-08-21 | Anthrogenesis Corporation | Hepatocytes and chondrocytes from adherent placental stem cells; and cd34+, cd45- placental stem cell-enriched cell populations |
| CN102282252A (zh) * | 2008-11-19 | 2011-12-14 | 人类起源公司 | 羊膜来源的贴壁细胞 |
| US20100124569A1 (en) * | 2008-11-19 | 2010-05-20 | Abbot Stewart | Amnion derived adherent cells |
| WO2010060080A1 (en) * | 2008-11-24 | 2010-05-27 | Immunotrex Corporation | Three dimensional tissue generation |
| WO2011064669A3 (en) * | 2009-11-30 | 2011-12-15 | Pluristem Ltd. | Adherent cells from placenta and use of same in disease treatment |
| US20110229970A1 (en) * | 2010-03-05 | 2011-09-22 | Florida State University Research Foundation | Dual-chamber perfusion bioreactor for orthopedic tissue interfaces and methods of use |
| WO2011154552A1 (en) * | 2010-06-11 | 2011-12-15 | Cellartis Ab | 3-dimensional scaffolds for improved differentiation of pluripotent stem cells to hepatocytes |
| CN103097517A (zh) * | 2010-06-11 | 2013-05-08 | 塞拉帝思股份公司 | 用于提高多能干细胞分化成肝细胞的3维支架 |
| WO2012083023A1 (en) * | 2010-12-17 | 2012-06-21 | Anthrogenesis Corporation | Treatment of spinal cord injury and traumatic brain injury using amnion derived adherent cells |
| WO2013040078A2 (en) * | 2011-09-12 | 2013-03-21 | Organovo, Inc. | Engineered tissues for in vitro research uses, arrays thereof, and methods of making the same |
Non-Patent Citations (1)
| Title |
|---|
| Enhanced cartilage tissue engineering by sequential exposure of chondrocytes to FGF-2 during 2D expansion and BMP-2 during 3D cultivation;Ivan Martin et al.;《Journal of cellular biochemistry》;20011231;第83卷;第121-128页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| JP6492106B2 (ja) | 2019-03-27 |
| EP3132023B1 (en) | 2021-06-02 |
| SG11201608463WA (en) | 2016-11-29 |
| AU2015248875B2 (en) | 2019-04-04 |
| WO2015158777A1 (en) | 2015-10-22 |
| CA2945548A1 (en) | 2015-10-22 |
| AU2015248875A1 (en) | 2016-10-27 |
| CA2945548C (en) | 2019-11-12 |
| GB201406716D0 (en) | 2014-05-28 |
| JP2017511146A (ja) | 2017-04-20 |
| US20230235277A1 (en) | 2023-07-27 |
| DK3132023T3 (da) | 2021-08-09 |
| EP3132023A1 (en) | 2017-02-22 |
| US20170037364A1 (en) | 2017-02-09 |
| CN106459895A (zh) | 2017-02-22 |
| AU2015248875B9 (en) | 2019-05-23 |
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