AU2015248875B9 - Method of preparing cells for 3D tissue culture - Google Patents

Method of preparing cells for 3D tissue culture Download PDF

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Publication number
AU2015248875B9
AU2015248875B9 AU2015248875A AU2015248875A AU2015248875B9 AU 2015248875 B9 AU2015248875 B9 AU 2015248875B9 AU 2015248875 A AU2015248875 A AU 2015248875A AU 2015248875 A AU2015248875 A AU 2015248875A AU 2015248875 B9 AU2015248875 B9 AU 2015248875B9
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Australia
Prior art keywords
cells
tissue culture
cell
tissue
culture
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Ceased
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AU2015248875A
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AU2015248875B2 (en
AU2015248875A1 (en
Inventor
Jens M. KELM
Jan Lichtenberg
Simon MESSNER
Wolfgang Moritz
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INSPHERO AG
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INSPHERO AG
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Publication of AU2015248875B2 publication Critical patent/AU2015248875B2/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062General methods for three-dimensional culture
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068General culture methods using substrates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/067Hepatocytes
    • C12N5/0671Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5014Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5067Liver cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/003D culture
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00Supports or coatings for cell culture, characterised by material

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2015248875A 2014-04-15 2015-04-15 Method of preparing cells for 3D tissue culture Ceased AU2015248875B9 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1406716.9A GB201406716D0 (en) 2014-04-15 2014-04-15 Method of preparing cells for 3D tissue culture
GB1406716.9 2014-04-15
PCT/EP2015/058174 WO2015158777A1 (en) 2014-04-15 2015-04-15 Method of preparing cells for 3d tissue culture

Publications (3)

Publication Number Publication Date
AU2015248875A1 AU2015248875A1 (en) 2016-10-27
AU2015248875B2 AU2015248875B2 (en) 2019-04-04
AU2015248875B9 true AU2015248875B9 (en) 2019-05-23

Family

ID=50844988

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2015248875A Ceased AU2015248875B9 (en) 2014-04-15 2015-04-15 Method of preparing cells for 3D tissue culture

Country Status (10)

Country Link
US (2) US20170037364A1 (https=)
EP (1) EP3132023B1 (https=)
JP (1) JP6492106B2 (https=)
CN (1) CN106459895B (https=)
AU (1) AU2015248875B9 (https=)
CA (1) CA2945548C (https=)
DK (1) DK3132023T3 (https=)
GB (1) GB201406716D0 (https=)
SG (1) SG11201608463WA (https=)
WO (1) WO2015158777A1 (https=)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6758026B2 (ja) * 2015-04-17 2020-09-23 株式会社日立ハイテク 成分分析装置、薬剤成分分析装置、成分分析方法及び薬剤成分分析方法
EP3397753B1 (en) * 2015-12-30 2021-10-27 FUJIFILM Cellular Dynamics, Inc. Microtissue formation using stem cell-derived human hepatocytes
GB201609663D0 (en) 2016-06-02 2016-07-20 Stemtek Therapeutics Sl Methods for producing cancer stem cell spheroids
WO2018162623A1 (en) * 2017-03-10 2018-09-13 Merck Patent Gmbh Infected cell cultures
CN109294971A (zh) * 2018-09-29 2019-02-01 西安交通大学 一种用于药物筛选的颈动脉分叉三维细胞培养模型及其构建方法
WO2020232436A1 (en) 2019-05-16 2020-11-19 Brown University A human in vitro cardiotoxicity model
DE102019216886A1 (de) 2019-10-31 2021-05-06 Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. Transfer zumindest eines partikels in einer flüssigkeit von einer abgabeeinheit zu einer empfangseinheit
CN111471641B (zh) * 2020-02-03 2021-11-05 东华大学 多片层单元水凝胶包被的仿生毛细血管网的3d打印制法
CN111150885A (zh) * 2020-03-05 2020-05-15 陕西佰傲干细胞再生医学有限公司 软骨微囊及其制备方法和应用
US12076437B2 (en) 2021-07-12 2024-09-03 Brown University Proangiogenic protein cocktails delivered in custom biomaterials to revascularize ischemic tissue
CN121969735A (zh) 2023-10-06 2026-05-01 银丝佛若有限公司 用于研究生物样本相互作用的装置

Citations (2)

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WO2008100497A1 (en) * 2007-02-12 2008-08-21 Anthrogenesis Corporation Hepatocytes and chondrocytes from adherent placental stem cells; and cd34+, cd45- placental stem cell-enriched cell populations
WO2011154552A1 (en) * 2010-06-11 2011-12-15 Cellartis Ab 3-dimensional scaffolds for improved differentiation of pluripotent stem cells to hepatocytes

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FR2545100B1 (fr) * 1983-04-29 1985-12-20 Inst Nat Sante Rech Med Procede d'obtention de cultures d'hepatocytes humains, les cultures obtenues et leurs applications biologiques et biochimiques
WO2003055487A1 (en) * 2002-01-04 2003-07-10 The University Of Adelaide METHOD OF CONTROLLING DAMAGE MEDIATED BY α, β- UNSATURATED ALDEHYDES
KR20040094910A (ko) * 2002-04-03 2004-11-10 아르테셀 사이언스, 인크. 개선된 지방세포 분화된 지방 유래 성체 줄기세포 및 이의용도
JP2004254674A (ja) * 2003-02-25 2004-09-16 Mutsumi Takagi 動物細胞の分化誘導培養方法
CN101506355B (zh) * 2004-09-24 2012-06-27 成血管细胞系统公司 多能扩增间充质前体细胞子代(memp)及其应用
WO2007124023A2 (en) * 2006-04-21 2007-11-01 Wake Forest University Health Sciences Ink-jet printing of tissues
ZA200902233B (en) * 2006-10-23 2010-07-28 Anthrogenesis Corp Methods and compositions for treatment of bone defects with placental cell populations
AU2009316541B2 (en) * 2008-11-19 2015-08-06 Celularity Inc. Amnion derived adherent cells
US20110250688A1 (en) * 2008-11-24 2011-10-13 Immunotrex Corporation Three Dimensional Tissue Generation
ES2609006T3 (es) * 2009-10-16 2017-04-18 Rutgers, The State University Of New Jersey Procedimeinto de tratamiento de lesión crónica de tejido nervioso usando una estrategia de terapia celular
EP2977445B1 (en) * 2009-11-30 2018-07-25 Pluristem Ltd. Adherent cells from placenta and use of same in disease treatment
US20110229970A1 (en) * 2010-03-05 2011-09-22 Florida State University Research Foundation Dual-chamber perfusion bioreactor for orthopedic tissue interfaces and methods of use
MX2013006592A (es) * 2010-12-17 2014-02-06 Anthrogenesis Corp Tratamiento de lesion de la medula espinal y lesion traumatica de cerebro empleando celulas adherentes obtenidas del amnios.
HK1200483A1 (en) * 2011-09-12 2015-08-07 奥加诺沃公司 Engineered tissues for in vitro research uses, arrays thereof, and methods of making the same

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008100497A1 (en) * 2007-02-12 2008-08-21 Anthrogenesis Corporation Hepatocytes and chondrocytes from adherent placental stem cells; and cd34+, cd45- placental stem cell-enriched cell populations
WO2011154552A1 (en) * 2010-06-11 2011-12-15 Cellartis Ab 3-dimensional scaffolds for improved differentiation of pluripotent stem cells to hepatocytes

Also Published As

Publication number Publication date
JP6492106B2 (ja) 2019-03-27
EP3132023B1 (en) 2021-06-02
CN106459895B (zh) 2021-06-01
SG11201608463WA (en) 2016-11-29
AU2015248875B2 (en) 2019-04-04
WO2015158777A1 (en) 2015-10-22
CA2945548A1 (en) 2015-10-22
AU2015248875A1 (en) 2016-10-27
CA2945548C (en) 2019-11-12
GB201406716D0 (en) 2014-05-28
JP2017511146A (ja) 2017-04-20
US20230235277A1 (en) 2023-07-27
DK3132023T3 (da) 2021-08-09
EP3132023A1 (en) 2017-02-22
US20170037364A1 (en) 2017-02-09
CN106459895A (zh) 2017-02-22

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