CN106420659B - A kind of bean-containing capsule and preparation method thereof - Google Patents
A kind of bean-containing capsule and preparation method thereof Download PDFInfo
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- CN106420659B CN106420659B CN201610968798.8A CN201610968798A CN106420659B CN 106420659 B CN106420659 B CN 106420659B CN 201610968798 A CN201610968798 A CN 201610968798A CN 106420659 B CN106420659 B CN 106420659B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
- A61K9/4841—Filling excipients; Inactive ingredients
- A61K9/4858—Organic compounds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
- A61K31/35—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom
- A61K31/352—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom condensed with carbocyclic rings, e.g. methantheline
- A61K31/353—3,4-Dihydrobenzopyrans, e.g. chroman, catechin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/63—Arthropods
- A61K35/64—Insects, e.g. bees, wasps or fleas
- A61K35/644—Beeswax; Propolis; Royal jelly; Honey
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/742—Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/43—Enzymes; Proenzymes; Derivatives thereof
- A61K38/46—Hydrolases (3)
- A61K38/48—Hydrolases (3) acting on peptide bonds (3.4)
- A61K38/482—Serine endopeptidases (3.4.21)
Abstract
The invention discloses a kind of bean-containing capsules, it is made of capsule shells and the content in capsule shells, wherein, content contains following component: 3-8 parts of median chain triglyceride oil, 0.1-0.5 parts of EGCG, 1-4 parts of Bacillus natto in parts by weight, 10-30 parts of Nattokinase, 50-75 parts of sunflower oil, 3-10 parts of content beeswax and 3-10 parts of fatty acid glyceride, capsule shells contain following component: 40-70 parts of gelatin, 15-30 parts of glycerol and 10-30 parts of capsule shells beeswax in parts by weight.The invention also discloses the preparation methods of the bean-containing capsule.The present invention dexterously combines above each ingredient of different role mechanism, make composition from the formation of inhibition thrombus, the dissolution of increase thrombus, accelerate the many-sided synergistic effect of elasticity of the thrombus discharge and enhancing blood vessel of dissolution, reaching makes human body become the constitution that will not be generated thrombus and fall the thrombolysis of formation and quickly the thrombus dissolved is excreted.
Description
Technical field
The invention belongs to medicines and health protection fields, and in particular to a kind of bean-containing capsule and preparation method thereof.
Background technique
Due to accumulating many pressure, disorder of variation and living habit of dietetic life etc., cause in our internal blood
Cholesterol and fat increase become muddy shape.Therefore it is easy to produce out thrombus, and blood component also disequilibrium, thus becomes
Even if thrombus occur also is difficult to the constitution dissolved.This thrombus is the cause of the major diseases such as myocardial infarction or brain gland.This
A little diseases no conscious sympton until falling ill mostly, it is most to be all suddenly seized with an illness, it even, can also there are sequelae.Therefore it is in
Among such social environment, mostly important is prevention work, will not generate thrombus and can be by shape to become
At the constitution fallen of thrombolysis, then must make the life better habit by movement and diet.
Natto is a kind of traditional food of Japan, is by hay bacillus (Bacillus subtilis) or Bacillus natto
(Bacillusnatto) ferment boiling soybean and a kind of manufactured functional food, has more than 2000 years edible history.Natto
It is raw containing some Nattokinases, isoflavones, lecithin, superoxide dismutase (SOD), unsaturated fatty acid and vitamin E etc.
Active material is managed, there is plurality of health care functions, often edible natto can not only effectively prevent and treat cardiovascular and cerebrovascular disease,
Also have the effects that anticancer, prevention and treatment osteoporosis, adjusts enteron aisle balance, is anti-oxidant.Especially Nattokinase is proved to have
Effect ground thrombus, mainly effectively dissolution insoluble fibrin, the albumen and cholesterol are all enhancing blood viscosities, make
At the substance of disorders of lipid metabolism, lipid material is deposited in vascular wall inner membrance, gradually forms small " patch ", medically known as " dynamic
Pulse atherosclerosis ".These patches increase, increase, and gradually block blood vessel, cause vessel lumen narrow, and blood circulation is unsmooth, if
Vitals cerebral arterial insufficiency, will lead to serious consequence.Usual serious cardiovascular and cerebrovascular atherosclerosis, can cause to be preced with
Heart trouble, myocardial infarction, angina pectoris, cerebral thrombosis, cerebral hemorrhage, apoplexy etc. or even threat to life.Since natto has preferable haemolysis
Bolt, blood pressure lowering and antitumor and other effects, therefore natto food is developed, good economic benefit and social benefit can be brought.However
Though natto itself has many healthcare functions, traditional Japanese Natto has ammonia odor taste and effect is relatively simple, and flavor is very
Embarrass Chinese to receive, furthermore existing natto product shelf life is short, absorptivity is not high, cannot make its effect bigizationner, make its
The effect of prevention and treatment cardiovascular and cerebrovascular disease is restricted.
EGCG (Epigallo-catechin gallate (EGCG)) is a kind of natural compounds that extracting and developing obtains from green tea
Object is most efficient active monomer component in tea polyphenols, has good safety and extensive physiological activity, has
Very strong inoxidizability acts as important role in terms of anticancer and cardiovascular disease.Studies have shown that EGCG has anti-blood
Bolt formation, anti arteriosclerosis, protection nervous system, free radical resisting damage, anti-oxidant, anti-oxidation stress, antiangiogenic, adjusting
Immune, protection cardiovascular and cerebrovascular and anti-inflammatory, antitumor, antiviral, antibacterial action, it is particularly significant for human health.
Flavone compound in propolis can play softening blood vessel, reduce fragility of blood vessels, effectively reduce thrombosis, prevent
Vascular sclerosis is controlled, the effect of microcirculation is improved.Not only flavones rich in but also there are many ingredient in propolis, there is similar dimension
The effect of raw element P sample reduces fragility of blood vessels equally to softening blood vessel, expands blood vessel, accelerates Hemodynamic environment, reduces red blood cell and blood
Platelet aggregation reduces and prevents thrombosis, and improving microcirculation has good effect.Therefore, propolis have " blood vessel scavenger " it
It says.
Application No. is 201310158684.3, the Chinese patent application of entitled " a kind of selenium-rich natto product " comprising
Following component and its parts by weight: 35-50 parts of selenium-rich natto powder, 10-16 parts of phytosterol powder, 10-15 parts of green tea extract powder,
The natto product combines selenium-rich natto powder, phytosterol powder and green tea extract powder together, from inhibition according to the ratio of science
The formation of thrombus, the dissolution for increasing thrombus, enhancing three aspect collective effect of vascular toughness, effectively prevent the hair of cardiovascular and cerebrovascular disease
It is raw, but in the natto product, since the intramolecular of EGCG has the carbolic acid water acidic group of plural number, so it is easily dissolved
In aqueous solvent, and it is difficult to be dissolved into oil-like solvent.Moreover, compared with by the oxidation in the oxygen and air deposited molten in water,
It is easier to be acidified, there is instability, be short of storage stability, and there is a problem of that selenium-rich natto powder absorptivity is low, so that
The excellent properties of EGCG and selenium-rich natto powder itself are unable to get sufficient use.
Summary of the invention
Single for natto food antithrombotic principle of the existing technology, poor storage stability and absorptivity be not high
Defect provides a kind of bean-containing capsule, while additionally providing the preparation method of the bean-containing capsule.
The purpose of the present invention is what is be achieved through the following technical solutions:
A kind of bean-containing capsule is made of capsule shells and the content being located in the capsule shells, and the content is with weight
It measures part meter and contains following component: 3-8 parts of median chain triglyceride oil, 0.1-0.5 parts of EGCG, 1-4 parts of Bacillus natto, Nattokinase 10-
30 parts, 45-75 parts of sunflower oil, 3-10 parts of beeswax and 3-10 parts of fatty acid glyceride, the capsule shells contain in parts by weight
Following component: 40-70 parts of gelatin, 15-30 parts of glycerol and 10-30 parts of beeswax.
Preferably, wherein the content of the GECG is not less than 98%.
Preferably, wherein the activity of the Nattokinase is not less than 1200FU.
Preferably, wherein the content contains following component: 5 parts of median chain triglyceride oil, EGCG in parts by weight
0.3 part, 3 parts of Bacillus natto, 20 parts of Nattokinase, 60 parts of sunflower oil, 3.7 parts of beeswax and 8 parts of fatty acid glyceride, the capsule
Shell contains following component: 45 parts of gelatin, 30 parts of glycerol and 25 parts of beeswax in parts by weight.
Preferably, wherein the content contains following component: 6.28 parts of median chain triglyceride oil, EGCG in parts by weight
0.42 part, 4 parts of Bacillus natto, 25 parts of Nattokinase, 45 parts of sunflower oil, 9.3 parts of beeswax and 10 parts of fatty acid glyceride, the glue
Softgel shell contains following component: 70 parts of gelatin, 20 parts of glycerol and 10 parts of beeswax in parts by weight.
Preferably, wherein the content contains following component: 5.8 parts of median chain triglyceride oil, EGCG in parts by weight
0.35 part, 3.2 parts of Bacillus natto, 15 parts of Nattokinase, 65 parts of sunflower oil, 6 parts of beeswax and fatty acid glyceride 4.65, the glue
Softgel shell contains following component: 40 parts of gelatin, 30 parts of glycerol and 30 parts of beeswax in parts by weight.
A kind of preparation method of bean-containing capsule comprising following steps:
(1) it weighs: weighing median chain triglyceride oil, EGCG, Bacillus natto, Nattokinase, sunflower oil, the content of formula ratio
Beeswax, capsule shells beeswax, fatty acid glyceride, gelatin and glycerol, it is spare；
(2) ingredient: Bacillus natto, Nattokinase, sunflower oil and fatty acid glyceride are uniformly mixed, mixture is obtained；
Content beeswax and EGCG are added when median chain triglyceride oil is heated to 60-90 DEG C, stirring melting 0.5-1 is small
When, oleosol is obtained, is then cooled to 15-30 DEG C of heat preservation, for use；
Above-mentioned mixture is added in oleosol, it is stirring while adding, it crosses colloid mill 2-3 times after mixing, takes off gas
Bubble, it is stand-by to obtain content；
(3) change glue: first will be heated to 50-70 DEG C in capsule shells beeswax, glycerol being put into glue tank, be 15-45rpm in revolving speed
Stirring under gelatin is slowly added to, and continue to be heated to 80-100 DEG C, stirring tanning 0.5-1 hour must after de-bubbled
To glue, and 80-120 mesh filters, and places 4-6 hours at 40-60 DEG C, spare；
(4) pressure watt: starting soft capsule press, carry out compacting capsule and pill, control the temperature and relative humidity of production environment, presses
The capsule and pill produced is transported in rotating cage with conveyer belt and is formed 3-5 hours；
(5) ball, drying are washed: carrying out cleaning capsule and pill with the edible alcohol that volumetric concentration is 15-95%, until capsule and pill surface light
Clean, bright, then capsule and pill is gone to hothouse drying by no grease, controls dry 24-30 DEG C of room temperature, relative humidity 20-
30%, it is 24-48 hours dry, soft capsule is obtained, soft capsule is fitted into bottle.
Preferably, in the step (4), the production environment temperature is 18-22 DEG C, relative humidity 40-60%, content
The loading amount of object is 0.3g/.
Compared with prior art, the present invention has at least the following advantages:
(1) bean-containing capsule provided by the present invention, wherein Nattokinase be proved to can effectively thrombus, mainly
It is to decompose cholesterol, acidification type lipid in decomposer, plays the role of inhibition thrombosis and thrombus, reduces cholesterol；
EGCG has effects that inhibit low-density albumen (insoluble fibrin) content in blood, and it is with stronger anti-oxidant
Property, the toughness of vascular wall is not only improved, the stability of the composition system is also extended；Not only Huang rich in propolis
Ketone but also there are many ingredient, makes it have softening blood vessel, reduces fragility of blood vessels, effectively reduce thrombosis, treatment of vascular is hard
Change, improves the effect of microcirculation, that is, increase the toughness of blood vessel, also accelerate the discharge of thrombus；Bacillus natto, which has, changes human body
Intestinal flora ecology, helps digest function normalization, so that defecation is smooth, maintains the effect of body physiological environmental protection, promotes
Microcirculation；Absorption pattern of the median chain triglyceride oil for human body is different from other greases, and infiltration rate is faster than long chain fatty acids
Four times, and it does not generate lipopexia, can dissolving cholesterol etc. well, reduce or avoid the wind of EGCG oxidation by air
Danger；Sunflower oil be in vegetable oil containing linoleic acid at most, linoleic acid have reduce blood lipid, softening blood vessel, reduce blood pressure, promote
The effect of microcirculation can play the deposition for preventing human serum cholesterol in vascular wall, enhance the toughness of blood vessel and promote micro- follow
Ring；And the addition of fatty acid glyceride further enhances the function of each component in system so that entire composition system is stablized
Effect.Above each ingredient of different role mechanism is dexterously combined, makes them by bean-containing capsule i.e. provided by the present invention
From the formation of thrombus, the dissolution of increase thrombus is inhibited, accelerate the thrombus discharge of dissolution and the elasticity various aspects collaboration of enhancing blood vessel
Effect, reaching, which becomes human body, will not generate thrombus, can fall the thrombolysis of formation and quickly the thrombus dissolved
The constitution excreted.
(2) bean-containing capsule provided by the present invention, by median chain triglyceride oil, EGCG, Bacillus natto, Nattokinase, sunflower
Oil, fatty acid glyceride and beeswax scientific matching, all raw material is green material, safe and efficient, while using capsule
It as carrier, carries and convenient, has evaded the bitter taste of EGCG and the bad smell of Nattokinase, take peace for a long time
Entirely, without toxic, side effects.
(3) it steadily disperses EGCG in median chain triglyceride oil in the present invention, so that EGCG is by three acid of medium chain triglyceride
The problem of ester wraps, and not only ensure that the meltage of EGCG, also avoids EGCG oxidation by air, so that the effect of EGCG can
Adequately to be used.
(4) bean-containing capsule provided by the present invention can significantly improve natto gum compared with the natto product of the prior art
The absorbability and stability of capsule, so that the resultant effect of each component is adequately embodied.
(5) bean-containing capsule provided by the present invention, since saturation of the beeswax in content be not high, beeswax is used respectively
In content component and capsule shells component, ratio of the beeswax in entire bean-containing capsule is improved, so that bean-containing capsule
With better antithrombotic property.
Specific embodiment
Below with reference to embodiment, the invention will be further described, and it is not limited that following embodiment, which is descriptive,
, this does not limit the scope of protection of the present invention.
Unless otherwise defined, all technical and scientific terms used herein has and those skilled in the art of the invention
Normally understood identical meanings.Contradiction if it exists, then be subject to definition provided by the present application.
Heretofore described content beeswax is the beeswax for content in bean-containing capsule, the capsule shells beeswax
For the beeswax for capsule shells in bean-containing capsule.
Unless otherwise stated, all percentage, parts by weight, ratio etc. herein are by weight.
Median chain triglyceride oil that the present invention uses, EGCG, Bacillus natto, Nattokinase, sunflower oil, fatty acid glyceride with
And beeswax is commercially available.
Embodiment 1
(1) it weighs: weighing 3 parts of median chain triglyceride oils, 0.1 part of EGCG, 1 part of Bacillus natto, 25 parts of Nattokinases, 60 portions of certain herbaceous plants with big flowers
Caul-fat, 5.9 parts of fatty acid glycerides, 5 parts of content beeswaxs, 30 parts of capsule shells beeswaxs, 50 parts of gelatin and 20 parts of glycerol, it is spare；
(2) ingredient: 1 part of Bacillus natto, 25 parts of Nattokinases, 60 portions of sunflower oils and 5.9 parts of fatty acid glycerides are mixed equal
It is even, obtain mixture；
5 parts of content beeswaxs of addition and 0.1 part of EGCG, stirring melt when 3 parts of median chain triglyceride oils are heated to 60 DEG C
0.5 hour, oleosol is obtained, is then cooled to 25 DEG C of heat preservations, for use；
Step (1) resulting mixture is sequentially added in factice liquid, it is stirring while adding, colloid mill 2- is crossed after mixing
3 times, bubble is taken off, obtains content, for use；
(3) change glue: first will be heated to 50 DEG C in 30 parts of capsule shells beeswaxs, 20 parts of glycerol being put into glue tanks, be in revolving speed
By 50 parts of gelatin being slowly added to glue tanks under the stirring of 15rpm, and continue to be heated to 80 DEG C, stirring tanning 0.5 hour takes off
Glue is obtained after bubble, the filtering of 80 mesh is placed 4 hours at 40 DEG C, spare；
(4) pressure watt: starting soft capsule press, carry out compacting capsule and pill, and 18 DEG C of production environment temperature, relative humidity 40%,
Control content 0.3g/, the capsule and pill suppressed is transported in rotating cage with conveyer belt and is formed 3 hours；
(5) ball, drying are washed: carrying out cleaning capsule and pill with the edible alcohol that volumetric concentration is 20%, until capsule and pill any surface finish,
Bright, then capsule and pill is gone to hothouse drying by no grease, controls dry 24 DEG C of room temperature, relative humidity 20%, drying 24
Hour, soft capsule is fitted into bottle, 60/bottle.
Embodiment 2:
(1) it weighs: weighing 5 parts of median chain triglyceride oils, 0.3 part of EGCG, 3 parts of Bacillus nattos, 20 parts of Nattokinases, 60 portions of certain herbaceous plants with big flowers
Caul-fat, 8 parts of fatty acid glycerides, 3.7 parts of content beeswaxs, 25 parts of capsule shells beeswaxs, 45 parts of gelatin and 30 parts of glycerol, it is spare；
(2) ingredient: 3 parts of Bacillus nattos, 20 parts of Nattokinases, 60 portions of sunflower oils and 8 parts of fatty acid glycerides are uniformly mixed,
Obtain mixture；
3.7 parts of content beeswaxs of addition and 0.3 part of EGCG, stirring are melted when 5 parts of median chain triglyceride oils are heated to 70 DEG C
Melt 0.8 hour, obtain oleosol, is then cooled to 20 DEG C of heat preservations, for use；
Step (1) resulting mixture is sequentially added in factice liquid, it is stirring while adding, colloid mill 2- is crossed after mixing
3 times, bubble is taken off, it is stand-by to obtain content；
(3) change glue: first will be heated to 60 DEG C in 25 parts of capsule shells beeswaxs, 30 parts of glycerol being put into glue tanks, be in revolving speed
By 45 parts of gelatin being slowly added to glue tanks under the stirring of 18rpm, and continue to be heated to 85 DEG C, stirring tanning 0.5 hour takes off
Glue is obtained after bubble, the filtering of 100 mesh is placed 4 hours at 50 DEG C, spare；
(4) pressure watt: starting soft capsule press, carry out compacting capsule and pill, and 20 DEG C of production environment temperature, relative humidity 45%,
Control content 0.3g/, the capsule and pill suppressed is transported in rotating cage with conveyer belt and is formed 3.5 hours；
(5) ball, drying are washed: carrying out cleaning capsule and pill with the edible alcohol that volumetric concentration is 25%, until capsule and pill any surface finish,
Bright, then capsule and pill is gone to hothouse drying by no grease, controls dry 27 DEG C of room temperature, relative humidity 25%, drying 30
Hour, soft capsule is fitted into bottle, 60/bottle.
Embodiment 3:
(1) weigh: weigh 4.74 parts of median chain triglyceride oils, 0.26 part of EGCG, 2.5 parts of Bacillus nattos, 30 parts of Nattokinases,
55 portions of sunflower oils, 4 parts of fatty acid glycerides, 3.5 parts of content beeswaxs, 20 parts of capsule shells beeswaxs, 55 parts of gelatin and 25 parts of glycerol,
It is spare；
(2) ingredient: 2.5 parts of Bacillus nattos, 30 parts of Nattokinases, 55 portions of sunflower oils and 4 parts of fatty acid glycerides are mixed equal
It is even, obtain mixture；
3.5 parts of content beeswaxs and 0.26 part of EGCG are added when 4.74 parts of median chain triglyceride oils are heated to 75 DEG C, is stirred
Melting 0.8 hour is mixed, oleosol is obtained, is then cooled to 20 DEG C of heat preservations, for use；
Step (1) resulting mixture is sequentially added in factice liquid, it is stirring while adding, colloid mill 2- is crossed after mixing
3 times, bubble is taken off, it is stand-by to obtain content；
(3) change glue: first will be heated to 58 DEG C in 20 parts of capsule shells beeswaxs, 25 parts of glycerol being put into glue tanks, be in revolving speed
By 55 parts of gelatin being slowly added to glue tanks under the stirring of 25rpm, and continue to be heated to 89 DEG C, stirring tanning 0.6 hour takes off
Glue is obtained after bubble, the filtering of 110 mesh is placed 6 hours at 50 DEG C, spare；
(4) pressure watt: starting soft capsule press, carry out compacting capsule and pill, and 20 DEG C of production environment temperature, relative humidity 52%,
Control content 0.3g/, the capsule and pill suppressed is transported in rotating cage with conveyer belt and is formed 4 hours；
(5) ball, drying are washed: carrying out cleaning capsule and pill with the edible alcohol that volumetric concentration is 30%, until capsule and pill any surface finish,
Bright, then capsule and pill is gone to hothouse drying by no grease, controls dry 27 DEG C of room temperature, relative humidity 23%, drying 32
Hour, soft capsule is fitted into bottle, 60/bottle.
Embodiment 4:
(1) it weighs: weighing 6.28 parts of median chain triglyceride oils, 0.42 part of EGCG, 4 parts of Bacillus nattos, 25 parts of Nattokinases, 45
Part sunflower oil, 10 parts of fatty acid glycerides, 9.3 parts of content beeswaxs, 10 parts of capsule shells beeswaxs, 70 parts of gelatin and 20 parts of glycerol,
It is spare；
(2) ingredient: 4 parts of Bacillus nattos, 25 parts of Nattokinases, 45 portions of sunflower oils and 10 parts of fatty acid glycerides are mixed equal
It is even, obtain mixture；
9.3 parts of content beeswaxs and 0.42 part of EGCG are added when 0.42 part of median chain triglyceride oil is heated to 75 DEG C, is stirred
Melting 1 hour is mixed, oleosol is obtained, is then cooled to 30 DEG C of heat preservations, for use；
Step (1) resulting mixture is sequentially added in factice liquid, it is stirring while adding, colloid mill 2- is crossed after mixing
3 times, bubble is taken off, it is stand-by to obtain content；
(3) change glue: first will be heated to 63 DEG C in 10 parts of capsule shells beeswaxs, 20 parts of glycerol being put into glue tanks, be in revolving speed
By 70 parts of gelatin being slowly added to glue tanks under the stirring of 30rpm, and continuing to be heated to 90 DEG C, stirring boils 0.65 hour,
Glue is obtained after de-bubbled, the filtering of 100 mesh is placed 6 hours at 50 DEG C, spare；
(4) pressure watt: starting soft capsule press, carry out compacting capsule and pill, and 22 DEG C of production environment temperature, relative humidity 60%,
Control content 0.3g/, the capsule and pill suppressed is transported in rotating cage with conveyer belt and is formed 4.5 hours；
(5) ball, drying are washed: carrying out cleaning capsule and pill with the edible alcohol that volumetric concentration is 42%, until capsule and pill any surface finish,
Bright, then capsule and pill is gone to hothouse drying by no grease, controls dry 30 DEG C of room temperature, relative humidity 30%, drying 42
Hour, soft capsule is fitted into bottle, 60/bottle.
Embodiment 5:
(1) it weighs: weighing 7.5 parts of median chain triglyceride oils, 0.5 part of EGCG, 3.5 parts of Bacillus nattos, 10 parts of Nattokinases, 70
Part sunflower oil, 3.5 parts of fatty acid glycerides, 5 parts of content beeswaxs, 15 parts of capsule shells beeswaxs, 60 parts of gelatin and 25 parts of glycerol, it is standby
With；
(2) ingredient: 3.5 parts of Bacillus nattos, 10 parts of Nattokinases, 70 portions of sunflower oils and 3.5 parts of fatty acid glycerides are mixed
Uniformly, mixture is obtained；
5 parts of content beeswaxs of addition and 0.5 part of EGCG, stirring are melted when 7.5 parts of median chain triglyceride oils are heated to 90 DEG C
Melt 0.5 hour, obtain oleosol, is then cooled to 20 DEG C of heat preservations, for use；
Step (1) resulting mixture is sequentially added in factice liquid, it is stirring while adding, colloid mill 2- is crossed after mixing
3 times, bubble is taken off, it is stand-by to obtain content；
(3) change glue: first will be heated to 58 DEG C in 15 parts of capsule shells beeswaxs, 25 parts of glycerol being put into glue tanks, be in revolving speed
By 60 parts of gelatin being slowly added to glue tanks under the stirring of 35rpm, and continue to be heated to 87 DEG C, stirring tanning 0.7 hour takes off
Glue is obtained after bubble, the filtering of 120 mesh is placed 4 hours at 50 DEG C, spare；
(4) pressure watt: starting soft capsule press, carry out compacting capsule and pill, and 20 DEG C of production environment temperature, relative humidity 50%,
Control content 0.3g/, the capsule and pill suppressed is transported in rotating cage with conveyer belt and is formed 5 hours；
(5) ball, drying are washed: carrying out cleaning capsule and pill with the edible alcohol that volumetric concentration is 60%, until capsule and pill any surface finish,
Bright, then capsule and pill is gone to hothouse drying by no grease, controls dry 26 DEG C of room temperature, relative humidity 27%, drying 36
Hour, soft capsule is fitted into bottle, 60/bottle.
Embodiment 6:
(1) weigh: weigh 5.8 parts of median chain triglyceride oils, 0.35 part of EGCG, 3.2 parts of Bacillus nattos, 15 parts of Nattokinases,
65 portions of sunflower oils, 4.65 parts of fatty acid glycerides, 6 parts of content beeswaxs, 30 parts of capsule shells beeswaxs, 40 parts of gelatin and 30 parts are sweet
Oil, it is spare；
(2) ingredient: 3.2 parts of Bacillus nattos, 15 parts of Nattokinases, 65 portions of sunflower oils and 4.65 parts of fatty acid glycerides are mixed
Uniformly, mixture is obtained；
6 parts of content beeswaxs of addition and 0.35 part of EGCG, stirring are melted when 5.8 parts of median chain triglyceride oils are heated to 85 DEG C
Melt 0.8 hour, obtain oleosol, is then cooled to 25 DEG C of heat preservations, for use；
Step (1) resulting mixture is sequentially added in factice liquid, it is stirring while adding, colloid mill 2- is crossed after mixing
3 times, bubble is taken off, it is stand-by to obtain content；
(3) change glue: first will be heated to 70 DEG C in 30 parts of capsule shells beeswaxs, 30 parts of glycerol being put into glue tanks, be in revolving speed
By 40 parts of gelatin being slowly added to glue tanks under the stirring of 40rpm, and continue to be heated to 80 DEG C, stirring tanning 0.5 hour takes off
Glue is obtained after bubble, the filtering of 100 mesh is placed 6 hours at 50 DEG C, spare；
(4) pressure watt: soft capsule press is started, compacting capsule and pill, 18-22 DEG C of production environment temperature, relative humidity 40- are carried out
60%, content 0.3g/ is controlled, the capsule and pill suppressed is transported in rotating cage with conveyer belt and is formed 5 hours；
(5) ball, drying are washed: carrying out cleaning capsule and pill with the edible alcohol that volumetric concentration is 83%, until capsule and pill any surface finish,
Bright, then capsule and pill is gone to hothouse drying by no grease, controls dry 30 DEG C of room temperature, relative humidity 28%, drying 45
Hour, soft capsule is fitted into bottle, 60/bottle.
Embodiment 7
In order to prove to be added merely EGCG in natto product, and it is added after median chain triglyceride oil oil is molten
The effect of EGCG, wherein being mixed to get by Bacillus natto, Nattokinase, sunflower oil, content beeswax and fatty acid glyceride mixed
Object, hereinafter referred to as natto essence are closed, the natto essence in this test specimen is prepared using the formula ratio in embodiment 5,
Then by the preparation method of the bean-containing capsule in any one of embodiment 1- embodiment 6, capsule material is prepared；
30 wistar rats will be randomly divided into 5 groups, every group 6, each group is respectively as follows:
1, blank group (Control) is gavaged and the same number of capsulae vacuus of administration group.
2, natto " Jinghuasu " essence capsule group (hereinafter referred to as sample 3), dosage 10kIUkg-1。
3, natto essence+EGCG Capsules group (hereinafter referred to as sample 1), dosage 10kIUkg-1。
4, natto essence+EGCG+ median chain triglyceride oil Capsules group (hereinafter referred to as sample 2), dosage 10kIU
kg-1。
Above-mentioned natto essence is prepared using the component and proportion of embodiment 5, i.e., receives in parts by weight containing 3.5 parts
Beans bacterium, 10 parts of Nattokinases, 70 portions of sunflower oils, 5 parts of content beeswaxs and 3.5 parts of fatty acid glycerides, and its capsule shells is with weight
It measures part meter and contains 15 parts of capsule shells beeswaxs, 60 parts of gelatin and 25 parts of glycerol, sample 1 is consistent with the EGCG content in sample 2, with weight
Part meter is measured containing 1 part of EGCG.
It is administered daily 1 time, continuous 7 days.The 8th day after administration, by rat with mass percent be 3.5% chloraldurate carry out
Intraperitoneal anesthesia (1ml/100g), operation separation bilateral common carotid arteries.Respectively pad a 1.2cm*3.0cm's under bilateral common carotid arteries
Plastic tab, with being impregnated with 50 μ l FeCl3Filter paper item (1.0cm*1.5cm) the covering Bilateral Cervical of solution (10%, w/w) always moves
Arteries and veins.Filter paper item is removed after 20min, cut color change interval and is cleaned with physiological saline, its length is measured after filter paper suck dry moisture, and
With analysis level precise weighing.
The weight of unit length thrombus is denoted as opposite bolt weight (mg/cm), and calculates anti-bolt rate (%) with following formula.
Anti- bolt rate %=(W control-W administration)/W control × 100%
The average opposite bolt weight of W control-control group；The average opposite bolt weight of W administration-administration group.
Anti- bolt rate is calculated by above formula, and statistical analysis is carried out to result, the results are shown in Table 1.
The anti-bolt rate result of 1 different dosing group of table
The result shows that illustrating that natto essence has significant anti-bolt by comparing to blank control group and sample 3
The anti-bolt effect of effect (P < 0.05), sample 1 and sample 2 is significantly stronger than sample 3, illustrates that natto can be enhanced in the addition of EGCG
The anti-bolt effect of product；And the anti-bolt effect of sample 2 is significantly higher than sample 1 again, illustrates to dissolve by median chain triglyceride oil
The EGCG arrived is with than having better anti-bolt effect with EGCG merely.
It follows that the EGCG that the substance absorbed is dissolved in median chain triglyceride oil is improved, this is because EGCG is by middle chain
The problem of triglyceride wraps, and not only ensure that the meltage of EGCG, also efficiently avoids EGCG oxidation by air, makes
Obtain the available sufficient use of effect of EGCG.
Embodiment 8 --- the stability of EGCG raising composition system
According to the preparation method of embodiment 7, sample 1, sample 2 and sample 3 are prepared, then sample 1, sample 2 and sample 3
It is respectively placed in 37 DEG C of environment, after 30 days, with the enzymatic activity of fibrin plate method measurement each sample, calculates remnant enzyme activity
Power.
The residual enzyme activity situation of 2 sample 1 of table, sample 2 and sample 3
| Group | Residual enzyme activity |
| Sample 1 | 53.6% |
| Sample 2 | 82% |
| Sample 3 | 28.9% |
As a result: after the composition powder of sample 1, sample 2 and sample 3 is placed 30 days under 37 DEG C of environment, residual enzyme activity
Respectively 53.6%, 82% and 28.9% illustrate that bean-containing capsule improves the temperature stability of NK.
More than, it is merely preferred embodiments of the present invention, but the protection scope invented is not limited thereto, it is any ripe
Know those skilled in the art in the technical scope disclosed by the present invention, any changes or substitutions that can be easily thought of, should all contain
Lid is within protection scope of the present invention.Therefore, the scope of protection of the invention shall be subject to the scope of protection specified in the patent claim.
Claims (8)
1. a kind of bean-containing capsule is made of capsule shells and the content being located in the capsule shells, it is characterised in that: in described
It is tolerant to contain following component: 3-8 parts of median chain triglyceride oil, EGCG0.1-0.5 parts, 1-4 parts of Bacillus natto, natto in parts by weight
10-30 parts of kinases, 45-75 parts of sunflower oil, 3-10 parts of content beeswax and 3-10 parts of fatty acid glyceride, the capsule shells with
Parts by weight meter contains following component: 40-70 parts of gelatin, 15-30 parts of glycerol and 10-30 parts of capsule shells beeswax.
2. bean-containing capsule according to claim 1, which is characterized in that the content of the EGCG is not less than 98%.
3. bean-containing capsule according to claim 1 or 2, which is characterized in that the activity of the Nattokinase is not less than
1200FU。
4. bean-containing capsule according to claim 1, which is characterized in that the content contains in parts by weight such as the following group
Point: 5 parts of median chain triglyceride oil, 0.3 part of EGCG, 3 parts of Bacillus natto, 20 parts of Nattokinase, 60 parts of sunflower oil, content beeswax
3.7 parts and 8 parts of fatty acid glyceride, the capsule shells contain following component in parts by weight: 45 parts of gelatin, 30 parts of glycerol with
And 25 parts of capsule shells beeswax.
5. bean-containing capsule according to claim 1, which is characterized in that the content contains in parts by weight such as the following group
Point: 6.28 parts of median chain triglyceride oil, 0.42 part of EGCG, 4 parts of Bacillus natto, 25 parts of Nattokinase, 45 parts of sunflower oil, content
9.3 parts of beeswax and 10 parts of fatty acid glyceride, the capsule shells contain following component: 70 parts of gelatin, glycerol in parts by weight
20 parts and 10 parts of capsule shells beeswax.
6. bean-containing capsule according to claim 1, which is characterized in that the content contains in parts by weight such as the following group
Point: 5.8 parts of median chain triglyceride oil, 0.35 part of EGCG, 3.2 parts of Bacillus natto, 15 parts of Nattokinase, sunflower oil 65, content bee
6 parts of wax and fatty acid glyceride 4.65, the capsule shells contain following component: 40 parts of gelatin, 30 parts of glycerol in parts by weight
And 30 parts of capsule shells beeswax.
7. a kind of preparation method of bean-containing capsule according to claim 1 to 6, it is characterised in that: this method packet
Include following steps:
(1) it weighs: weighing median chain triglyceride oil, EGCG, Bacillus natto, Nattokinase, the sunflower oil, content bee of formula ratio
Wax, capsule shells beeswax, fatty acid glyceride, gelatin and glycerol, it is spare；
(2) ingredient: Bacillus natto, Nattokinase, sunflower oil and fatty acid glyceride are uniformly mixed, mixture is obtained；
Addition content beeswax and EGCG, stirring melting 0.5-1 hours obtain when median chain triglyceride oil is heated to 60-90 DEG C
To oleosol, it is then cooled to 15-30 DEG C of heat preservation, for use；
Above-mentioned mixture is added in oleosol, it is stirring while adding, it crosses colloid mill 2-3 times after mixing, takes off bubble, obtain
Content is stand-by；
(3) change glue: first will be heated to 50-70 DEG C in capsule shells beeswax, glycerol being put into glue tank, be stirring for 15-45rpm in revolving speed
It mixes and is slowly added to gelatin under state, and continue to be heated to 80-100 DEG C, stirring tanning 0.5-1 hours obtains glue after de-bubbled
Liquid, and 80-120 mesh filters, and places 4-6 hours at 40-60 DEG C, it is spare；
(4) pressure watt: soft capsule press is started, compacting capsule and pill is carried out, the temperature and relative humidity of production environment is controlled, suppresses
Capsule and pill be transported in rotating cage and be formed 3-5 hours with conveyer belt；
(5) ball, drying are washed: carrying out cleaning capsule and pill with the edible alcohol that volumetric concentration is 15-95%, until capsule and pill any surface finish,
Bright, then capsule and pill is gone to hothouse drying by no grease, controlling dry room temperature is 24-30 DEG C, relative humidity 20-
30%, it is 24-48 hours dry, soft capsule is obtained, soft capsule is fitted into bottle.
8. the preparation method of bean-containing capsule according to claim 7, which is characterized in that in step (4), the production environment
Temperature is 18-22 DEG C, relative humidity 40-60%, and the loading amount of content is 0.3g/.
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| CN101117630A (en) * | 2006-07-31 | 2008-02-06 | 北京小西保健食品有限公司 | Preparation method of nattokinase powder and natto Brazil mushroom capsule |
| CN101214263A (en) * | 2007-12-26 | 2008-07-09 | 浙江大学宁波理工学院 | Method for preparing freeze-dried powder containing bacillus natto and nattokinase |
| CN102138660A (en) * | 2010-02-02 | 2011-08-03 | 梁刚 | Natto capsules and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101117630A (en) * | 2006-07-31 | 2008-02-06 | 北京小西保健食品有限公司 | Preparation method of nattokinase powder and natto Brazil mushroom capsule |
| CN101214263A (en) * | 2007-12-26 | 2008-07-09 | 浙江大学宁波理工学院 | Method for preparing freeze-dried powder containing bacillus natto and nattokinase |
| CN102138660A (en) * | 2010-02-02 | 2011-08-03 | 梁刚 | Natto capsules and preparation method thereof |
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