CN106420659A - Natto capsule and preparation method thereof - Google Patents
Natto capsule and preparation method thereof Download PDFInfo
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- CN106420659A CN106420659A CN201610968798.8A CN201610968798A CN106420659A CN 106420659 A CN106420659 A CN 106420659A CN 201610968798 A CN201610968798 A CN 201610968798A CN 106420659 A CN106420659 A CN 106420659A
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Abstract
The invention discloses a natto capsule. The natto capsule comprises a capsule shell and contents in the capsule shell, wherein the contents comprise components in parts by weight as follows: 3-8 parts of medium chain triglycerides, 0.1-0.5 parts of EGCG (epigallocatechin gallate), 1-4 parts of bacillus natto, 10-30 parts of nattokinase, 50-75 parts of sunflower oil, 3-10 parts of content beewax and 3-10 parts of glycerin fatty acid ester; the capsule shell is prepared from components in parts by weight as follows: 40-70 parts of gelatin, 15-30 parts of glycerin and 10-30 parts of capsule shell beewax. The invention further discloses a preparation method of the natto capsule. The components with different action mechanisms are combined ingeniously, so that composition has a synergistic effect in the aspects of inhibiting thrombus formation, accelerating thromboclasis, accelerating expelling of dissolved thrombus and enhancing elasticity of blood vessels, and the constitution of a human body is endowed with functions of preventing thrombus formation, dissolving formed thrombus and expelling the dissolved thrombus out of the body rapidly.
Description
Technical field
The invention belongs to medicines and health protection field is and in particular to a kind of bean-containing capsule and preparation method thereof.
Background technology
Due to accumulating disorder of many pressure, the change of dietetic life and habits and customs etc., lead in our internal blood
Cholesterol and fat increase become muddy shape.Therefore easily produce thrombus, and blood component also disequilibrium, thus become
Even if the physique that thrombus is also difficult to dissolve occurs.This thrombus is the cause of the major disease such as miocardial infarction or brain gland.This
A little diseases no conscious sympton to morbidity mostly, majority is all suddenly seized with an illness, or even, also can leave sequelae.Therefore it is in
Among such social environment, of paramount importance be prevention work, thrombus and can be by shape will not be produced in order to become
The physique fallen of thrombolysis becoming, then must make the life better custom by motion and diet.
Natto is a kind of traditional food of Japan, is by hay bacillus (Bacillus subtilis) or Bacillus natto
(Bacillusnatto) fermentation boiling soybean and a kind of functional food of making, the edible history of existing more than 2000 year.Natto
Raw containing some Nattokinases, isoflavones, lecithin, superoxide dismutase (SOD), unrighted acid and vitamin E etc.
Reason active material, has plurality of health care functions, often eats natto and effectively can not only prevent and treat cardiovascular and cerebrovascular disease,
Also there is anticancer, prevent and treat osteoporosis, regulating intestinal canal balance, the effect such as anti-oxidant.Especially Nattokinase is proved to have
Effect ground thrombus, mainly effectively dissolve insoluble fibrin, this albumen and cholesterol are all to strengthen blood viscosity, make
Become the material of disorders of lipid metabolism, lipid material deposits in vascular wall inner membrance, gradually forms little " patch ", medically known as " dynamic
Pulse atherosclerosis ".These patches increase, increase, and gradually block blood vessel, cause vessel lumen narrow, blood circulation is not smooth, if
Vitals cerebral arterial insufficiency, may result in serious consequence.Generally serious cardiovascular and cerebrovascular atherosclerotic, can cause hat
Worry, miocardial infarction, angina pectoris, cerebral thrombus, cerebral hemorrhage, apoplexy etc., or even threat to life.Because natto has preferable haemolysis
Bolt, hypotensive and antitumor the effects such as, therefore develop natto food, good economic benefit and social benefit can be brought.But
Though natto itself has many health cares, traditional Japanese Natto carries ammonia stink and effectiveness comparison is single, and its local flavor is very
Embarrass Chinese to accept, existing natto product shelf life is short in addition, absorptivity is not high it is impossible to make its effect bigizationner so as to
The effect of preventing and treating cardiovascular and cerebrovascular disease is restricted.
EGCG (Epigallo-catechin gallate (EGCG)) is a kind of natural compounds that extracting and developing obtains from green tea
Thing, is the most efficient active monomer component in Tea Polyphenols, has good security and extensive physiological activity, it has
Very strong non-oxidizability, act as important role in terms of anticancer and angiocardiopathy.Research shows, EGCG has anti-blood
Bolt formation, anti arteriosclerosis, protection nervous system, Green Tea Extract infringement, anti-oxidant, anti-oxidation stress, antiangiogenic, regulation
Immunity, protection cardiovascular and cerebrovascular and anti-inflammatory, antitumor, antiviral, antibacterial action, particularly significant for health.
Flavone compound in propolis can play softening blood vessel, reduces fragility of blood vessels, effectively reduces thrombosis, prevents
Control vascular sclerosis, improve microcirculatory effect.Do not contain only abundant flavones in propolis and also Multiple components, have similar dimension
The effect of raw element P sample, equally to softening blood vessel, reduces fragility of blood vessels, expansion of blood vessels, accelerates Hemodynamic environment, reduce red blood cell and blood
Platelet is assembled, and reduces and stops thrombosis, improving microcirculation has good effect.Therefore, propolis have " blood vessel scavenger " it
Say.
Application No. 201310158684.3, the Chinese patent application of entitled " a kind of selenium-rich natto product ", it includes
Following component and its parts by weight：Selenium-rich natto powder 35-50 part, phytosterol powder 10-16 part, green tea extract powder 10-15 part,
This natto product by selenium-rich natto powder, phytosterol powder and green tea extract powder according to science ratio combine together with, from suppression
The formation of thrombus, the dissolving increasing thrombus, enhancing vascular toughness three aspect collective effect, effectively prevent sending out of cardiovascular and cerebrovascular disease
Raw, but in this natto product, because the intramolecular of EGCG has the carbolic acid water acidic group of plural number, so it is easily dissolved
In aqueous solvent, and it is difficult to be dissolved in oil-like solvent.And, compared with by the oxidation of the oxygen deposited molten in water and in the air, its
Be easier to be acidified, have an instability, be short of storage stability, and there is a problem of selenium-rich natto powder absorptivity low so that
EGCG and the selenium-rich natto powder excellent properties of itself cannot sufficiently be used.
Content of the invention
The natto food antithrombotic principle existing for prior art is single, and poor storage stability and absorptivity be not high
A kind of defect, there is provided bean-containing capsule, additionally provides the preparation method of this bean-containing capsule simultaneously.
The purpose of the present invention is achieved through the following technical solutions：
A kind of bean-containing capsule, is made up of with the content being located in described capsule shells capsule shells, and described content is with weight
Amount part meter contains following component：Median chain triglyceride oil 3-8 part, EGCG 0.1-0.5 part, Bacillus natto 1-4 part, Nattokinase 10-
30 parts, sunflower oil 45-75 part, beeswax 3-10 part and fatty acid glyceride 3-10 part, described capsule shells contain in parts by weight
Following component：Gelatin 40-70 part, glycerine 15-30 part and beeswax 10-30 part.
Preferably, the content of wherein said GECG is not less than 98%.
Preferably, the activity of wherein said Nattokinase is not less than 1200FU.
Preferably, wherein said content contains following component in parts by weight：5 parts of median chain triglyceride oil, EGCG
0.3 part, 3 parts of Bacillus natto, 20 parts of Nattokinase, 60 parts of sunflower oil, 3.7 parts of beeswax and 8 parts of fatty acid glyceride, described capsule
Shell contains following component in parts by weight：45 parts of gelatin, 30 parts of glycerine and 25 parts of beeswax.
Preferably, wherein said content contains following component in parts by weight：6.28 parts of median chain triglyceride oil, EGCG
0.42 part, 4 parts of Bacillus natto, 25 parts of Nattokinase, 45 parts of sunflower oil, 9.3 parts of beeswax and 10 parts of fatty acid glyceride, described glue
Softgel shell contains following component in parts by weight：70 parts of gelatin, 20 parts of glycerine and 10 parts of beeswax.
Preferably, wherein said content contains following component in parts by weight：5.8 parts of median chain triglyceride oil, EGCG
0.35 part, 3.2 parts of Bacillus natto, 15 parts of Nattokinase, 65 parts of sunflower oil, 6 parts of beeswax and fatty acid glyceride 4.65, described glue
Softgel shell contains following component in parts by weight：40 parts of gelatin, 30 parts of glycerine and 30 parts of beeswax.
A kind of preparation method of bean-containing capsule, it comprises the steps：
(1) weigh：Weigh the median chain triglyceride oil of formula ratio, EGCG, Bacillus natto, Nattokinase, sunflower oil, content
Beeswax, capsule shells beeswax, fatty acid glyceride, gelatin and glycerine, standby；
(2) dispensing：Bacillus natto, Nattokinase, sunflower oil and fatty acid glyceride are mixed, obtains mixture；
Median chain triglyceride oil is heated to when 60-90 DEG C and adds content beeswax and EGCG, melting 0.5-1 is little for stirring
When, obtain oleosol, be then cooled to 15-30 DEG C of insulation, stand-by；
Above-mentioned mixture is added in oleosol, stirring while adding, cross colloid mill 2-3 time after mixing, take off gas
Bubble, obtains content stand-by；
(3) change glue：First 50-70 DEG C will be heated in capsule shells beeswax, glycerine putting into glue tank, be 15-45rpm in rotating speed
Stirring under gelatin is slowly added to, and continue to be heated to 80-100 DEG C, stirring boils 0.5-1 hour, after de-bubbled
To glue, and 80-120 mesh filters, and places 4-6 hour at 40-60 DEG C, standby；
(4) pressure watt：Start soft capsule press, carry out suppressing capsule and pill, control temperature and the relative humidity of production environment, pressure
The capsule and pill tape transport made is shaped 3-5 hour in rotating cage；
(5) ball, drying are washed：It is carried out capsule and pill with the edible alcohol that volumetric concentration is 15-95%, until capsule and pill surface light
Clean, bright, no grease, then capsule and pill is gone to hothouse and be dried, control and room temperature 24-30 DEG C, relative humidity 20- are dried
30%, 24-48 hour is dried, obtains soft capsule, soft capsule is loaded in bottle.
Preferably, in described step (4), described production environment temperature is 18-22 DEG C, and relative humidity is 40-60%, content
The loading amount of thing is 0.3g/ grain.
Compared with prior art, the present invention at least has advantages below：
(1) bean-containing capsule provided by the present invention, wherein, Nattokinase be proved to can thrombus effectively, mainly
It is to decompose cholesterol, decompose internal acidifying type lipid, play the role of inhibition thrombosis and thrombus, reduce cholesterol；
EGCG has functions that to suppress low-density albumen (insoluble fibrin) content in blood, and its have stronger anti-oxidant
Property, not only improve the toughness of vascular wall, also extend the stability of said composition system；Abundant Huang is not contained only in propolis
Ketone and also Multiple components so as to have softening blood vessel, reduce fragility of blood vessels, effectively reduce thrombosis, treatment of vascular is hard
Change, improve microcirculatory effect, that is, increased the toughness of blood vessel, also accelerate the discharge of thrombus；Bacillus natto has change human body
Intestinal flora is ecological, helps digest function normalization, so that defecation is smooth, maintains the effect of body physiological environmental protection, promotes
Microcirculation；The absorption pattern that median chain triglyceride oil is used for human body is different from other greases, and infiltration rate is faster than LCFA
Four times, and it does not produce lipopexia, can dissolving cholesterol etc. well, reduce or avoid the wind of EGCG oxidation by air
Danger；Sunflower oil is most containing linoleic acid in vegetable oil, and linoleic acid has reduction blood fat, softening blood vessel, reduces blood pressure, promotes
Microcirculatory effect, can play the deposition preventing human serum cholesterol in vascular wall, strengthen the toughness of blood vessel and promote micro- following
Ring；And the addition of fatty acid glyceride, so that whole composition system is stable, further enhancing the work(of each component in system
Effect.I.e. bean-containing capsule provided by the present invention, above each composition of the different mechanisms of action is dexterously combined, makes them
From suppressing the formation of thrombus, the dissolving of increase thrombus, accelerate the thrombus discharge of dissolving and the elastic many-sided collaborative of enhancing blood vessel
Effect, reaching makes human body become will not to produce thrombus, can fall the thrombolysis of formation and quickly the thrombus dissolving
The physique excreting.
(2) bean-containing capsule provided by the present invention, by median chain triglyceride oil, EGCG, Bacillus natto, Nattokinase, sunflower
Oil, fatty acid glyceride and beeswax scientific matching, all raw material is all green material, safe and efficient, adopts capsule simultaneously
As carrier, carry and instant, evaded the bitter taste of EGCG and the bad smell of Nattokinase, long-term taking is pacified
Entirely, no toxicity, side effect.
(3) in the present invention, EGCG is stably scattered in median chain triglyceride oil so that EGCG is by medium chain triglyceride three acid
Ester wraps, and not only ensure that the meltage of EGCG, it also avoid the problem of EGCG oxidation by air so that the effect of EGCG can
Sufficiently to be used.
(4) bean-containing capsule provided by the present invention, compared with the natto product of prior art, can significantly improve natto gum
The absorbability of capsule and stability are so that the resultant effect of each component is sufficiently embodied.
(5) bean-containing capsule provided by the present invention, because saturation in content for the beeswax is not high, beeswax is used respectively
In content component and capsule shells component, improve ratio in whole bean-containing capsule for the beeswax, so that bean-containing capsule
There is more preferable antithrombotic property.
Specific embodiment
With reference to embodiment, the invention will be further described, and following examples are descriptive, is not limited
It is impossible to protection scope of the present invention is limited with this.
Unless otherwise defined, all technology used herein and scientific terminology have and those skilled in the art of the invention
The identical meanings being generally understood that.If there is contradiction, it is defined by the definition that the application provides.
Heretofore described content beeswax is the beeswax for content in bean-containing capsule, described capsule shells beeswax
It is the beeswax for capsule shells in bean-containing capsule.
Unless otherwise indicated, all of percentage, parts by weight, ratio etc. are all by weight herein.
The present invention use median chain triglyceride oil, EGCG, Bacillus natto, Nattokinase, sunflower oil, fatty acid glyceride with
And beeswax be commercial.
Embodiment 1
(1) weigh：Weigh 3 parts of median chain triglyceride oils, 0.1 portion of EGCG, 1 portion of Bacillus natto, 25 portions of Nattokinases, 60 portions of certain herbaceous plants with big flowers
Caul-fat, 5.9 parts of fatty acid glycerides, 5 parts of content beeswaxs, 30 parts of capsule shells beeswaxs, 50 parts of gelatin and 20 parts of glycerine, standby；
(2) dispensing：By 1 part of Bacillus natto, 25 parts of Nattokinases, 60 portions of sunflower oils and 5.9 parts of fatty acid glyceride mixing all
Even, obtain mixture；
3 parts of median chain triglyceride oils are heated to when 60 DEG C and add 5 parts of content beeswaxs and 0.1 part of EGCG, stirring melting
0.5 hour, obtain oleosol, be then cooled to 25 DEG C of insulations, stand-by；
The mixture of step (1) gained is sequentially added in factice liquid, stirring while adding, cross colloid mill 2- after mixing
3 times, take off bubble, obtain content, stand-by；
(3) change glue：First 50 DEG C will be heated in 30 parts of capsule shells beeswaxs, 20 parts of glycerine putting into glue tanks, in rotating speed be
By 50 parts of gelatin being slowly added to glue tanks under the stirring of 15rpm, and continue to be heated to 80 DEG C, stirring boils 0.5 hour, take off
Obtain glue after bubble, 80 mesh filter, place 4 hours at 40 DEG C, standby；
(4) pressure watt：Start soft capsule press, carry out suppress capsule and pill, 18 DEG C of production environment temperature, relative humidity 40%,
Control content thing 0.3g/ grain, the capsule and pill tape transport suppressing is shaped 3 hours in rotating cage；
(5) ball, drying are washed：It is carried out capsule and pill with the edible alcohol that volumetric concentration is 20%, until capsule and pill any surface finish,
Bright, no grease, then capsule and pill is gone to hothouse and be dried, control and room temperature 24 DEG C is dried, relative humidity 20%, it is dried 24
Hour, soft capsule is loaded in bottle, 60/bottle.
Embodiment 2：
(1) weigh：Weigh 5 parts of median chain triglyceride oils, 0.3 portion of EGCG, 3 portions of Bacillus nattos, 20 portions of Nattokinases, 60 portions of certain herbaceous plants with big flowers
Caul-fat, 8 parts of fatty acid glycerides, 3.7 parts of content beeswaxs, 25 parts of capsule shells beeswaxs, 45 parts of gelatin and 30 parts of glycerine, standby；
(2) dispensing：3 parts of Bacillus nattos, 20 parts of Nattokinases, 60 portions of sunflower oils and 8 parts of fatty acid glycerides are mixed,
Obtain mixture；
5 parts of median chain triglyceride oils are heated to when 70 DEG C and add 3.7 parts of content beeswaxs and 0.3 part of EGCG, stirring is molten
Melt 0.8 hour, obtain oleosol, be then cooled to 20 DEG C of insulations, stand-by；
The mixture of step (1) gained is sequentially added in factice liquid, stirring while adding, cross colloid mill 2- after mixing
3 times, take off bubble, obtain content stand-by；
(3) change glue：First 60 DEG C will be heated in 25 parts of capsule shells beeswaxs, 30 parts of glycerine putting into glue tanks, in rotating speed be
By 45 parts of gelatin being slowly added to glue tanks under the stirring of 18rpm, and continue to be heated to 85 DEG C, stirring boils 0.5 hour, take off
Obtain glue after bubble, 100 mesh filter, place 4 hours at 50 DEG C, standby；
(4) pressure watt：Start soft capsule press, carry out suppress capsule and pill, 20 DEG C of production environment temperature, relative humidity 45%,
Control content thing 0.3g/ grain, the capsule and pill tape transport suppressing is shaped 3.5 hours in rotating cage；
(5) ball, drying are washed：It is carried out capsule and pill with the edible alcohol that volumetric concentration is 25%, until capsule and pill any surface finish,
Bright, no grease, then capsule and pill is gone to hothouse and be dried, control and room temperature 27 DEG C is dried, relative humidity 25%, it is dried 30
Hour, soft capsule is loaded in bottle, 60/bottle.
Embodiment 3：
(1) weigh：Weigh 4.74 parts of median chain triglyceride oils, 0.26 part of EGCG, 2.5 parts of Bacillus nattos, 30 parts of Nattokinases,
55 portions of sunflower oils, 4 parts of fatty acid glycerides, 3.5 parts of content beeswaxs, 20 parts of capsule shells beeswaxs, 55 parts of gelatin and 25 parts of glycerine,
Standby；
(2) dispensing：By 2.5 parts of Bacillus nattos, 30 parts of Nattokinases, 55 portions of sunflower oils and 4 parts of fatty acid glyceride mixing all
Even, obtain mixture；
4.74 parts of median chain triglyceride oils are heated to when 75 DEG C and add 3.5 parts of content beeswaxs and 0.26 part of EGCG, stir
Mix melting 0.8 hour, obtain oleosol, be then cooled to 20 DEG C of insulations, stand-by；
The mixture of step (1) gained is sequentially added in factice liquid, stirring while adding, cross colloid mill 2- after mixing
3 times, take off bubble, obtain content stand-by；
(3) change glue：First 58 DEG C will be heated in 20 parts of capsule shells beeswaxs, 25 parts of glycerine putting into glue tanks, in rotating speed be
By 55 parts of gelatin being slowly added to glue tanks under the stirring of 25rpm, and continue to be heated to 89 DEG C, stirring boils 0.6 hour, take off
Obtain glue after bubble, 110 mesh filter, place 6 hours at 50 DEG C, standby；
(4) pressure watt：Start soft capsule press, carry out suppress capsule and pill, 20 DEG C of production environment temperature, relative humidity 52%,
Control content thing 0.3g/ grain, the capsule and pill tape transport suppressing is shaped 4 hours in rotating cage；
(5) ball, drying are washed：It is carried out capsule and pill with the edible alcohol that volumetric concentration is 30%, until capsule and pill any surface finish,
Bright, no grease, then capsule and pill is gone to hothouse and be dried, control and room temperature 27 DEG C is dried, relative humidity 23%, it is dried 32
Hour, soft capsule is loaded in bottle, 60/bottle.
Embodiment 4：
(1) weigh：Weigh 6.28 parts of median chain triglyceride oils, 0.42 part of EGCG, 4 parts of Bacillus nattos, 25 parts of Nattokinases, 45
Part sunflower oil, 10 parts of fatty acid glycerides, 9.3 parts of content beeswaxs, 10 parts of capsule shells beeswaxs, 70 parts of gelatin and 20 parts of glycerine,
Standby；
(2) dispensing：By 4 parts of Bacillus nattos, 25 parts of Nattokinases, 45 portions of sunflower oils and 10 parts of fatty acid glyceride mixing all
Even, obtain mixture；
0.42 part of median chain triglyceride oil is heated to when 75 DEG C and adds 9.3 parts of content beeswaxs and 0.42 part of EGCG, stir
Mix melting 1 hour, obtain oleosol, be then cooled to 30 DEG C of insulations, stand-by；
The mixture of step (1) gained is sequentially added in factice liquid, stirring while adding, cross colloid mill 2- after mixing
3 times, take off bubble, obtain content stand-by；
(3) change glue：First 63 DEG C will be heated in 10 parts of capsule shells beeswaxs, 20 parts of glycerine putting into glue tanks, in rotating speed be
By 70 parts of gelatin being slowly added to glue tanks under the stirring of 30rpm, and continue to be heated to 90 DEG C, stirring boils 0.65 hour,
Obtain glue after de-bubbled, 100 mesh filter, place 6 hours at 50 DEG C, standby；
(4) pressure watt：Start soft capsule press, carry out suppress capsule and pill, 22 DEG C of production environment temperature, relative humidity 60%,
Control content thing 0.3g/ grain, the capsule and pill tape transport suppressing is shaped 4.5 hours in rotating cage；
(5) ball, drying are washed：It is carried out capsule and pill with the edible alcohol that volumetric concentration is 42%, until capsule and pill any surface finish,
Bright, no grease, then capsule and pill is gone to hothouse and be dried, control and room temperature 30 DEG C is dried, relative humidity 30%, it is dried 42
Hour, soft capsule is loaded in bottle, 60/bottle.
Embodiment 5：
(1) weigh：Weigh 7.5 parts of median chain triglyceride oils, 0.5 part of EGCG, 3.5 parts of Bacillus nattos, 10 parts of Nattokinases, 70
Part sunflower oil, 3.5 parts of fatty acid glycerides, 5 parts of content beeswaxs, 15 parts of capsule shells beeswaxs, 60 parts of gelatin and 25 parts of glycerine, standby
With；
(2) dispensing：By 3.5 parts of Bacillus nattos, 10 parts of Nattokinases, 70 portions of sunflower oils and 3.5 parts of fatty acid glyceride mixing
Uniformly, obtain mixture；
7.5 parts of median chain triglyceride oils are heated to when 90 DEG C and add 5 parts of content beeswaxs and 0.5 part of EGCG, stirring is molten
Melt 0.5 hour, obtain oleosol, be then cooled to 20 DEG C of insulations, stand-by；
The mixture of step (1) gained is sequentially added in factice liquid, stirring while adding, cross colloid mill 2- after mixing
3 times, take off bubble, obtain content stand-by；
(3) change glue：First 58 DEG C will be heated in 15 parts of capsule shells beeswaxs, 25 parts of glycerine putting into glue tanks, in rotating speed be
By 60 parts of gelatin being slowly added to glue tanks under the stirring of 35rpm, and continue to be heated to 87 DEG C, stirring boils 0.7 hour, take off
Obtain glue after bubble, 120 mesh filter, place 4 hours at 50 DEG C, standby；
(4) pressure watt：Start soft capsule press, carry out suppress capsule and pill, 20 DEG C of production environment temperature, relative humidity 50%,
Control content thing 0.3g/ grain, the capsule and pill tape transport suppressing is shaped 5 hours in rotating cage；
(5) ball, drying are washed：It is carried out capsule and pill with the edible alcohol that volumetric concentration is 60%, until capsule and pill any surface finish,
Bright, no grease, then capsule and pill is gone to hothouse and be dried, control and room temperature 26 DEG C is dried, relative humidity 27%, it is dried 36
Hour, soft capsule is loaded in bottle, 60/bottle.
Embodiment 6：
(1) weigh：Weigh 5.8 parts of median chain triglyceride oils, 0.35 part of EGCG, 3.2 parts of Bacillus nattos, 15 parts of Nattokinases,
65 portions of sunflower oils, 4.65 parts of fatty acid glycerides, 6 parts of content beeswaxs, 30 parts of capsule shells beeswaxs, 40 parts of gelatin and 30 parts are sweet
Oil, standby；
(2) dispensing：By 3.2 parts of Bacillus nattos, 15 parts of Nattokinases, 65 portions of sunflower oils and 4.65 parts of fatty acid glyceride mixing
Uniformly, obtain mixture；
5.8 parts of median chain triglyceride oils are heated to when 85 DEG C and add 6 parts of content beeswaxs and 0.35 part of EGCG, stirring is molten
Melt 0.8 hour, obtain oleosol, be then cooled to 25 DEG C of insulations, stand-by；
The mixture of step (1) gained is sequentially added in factice liquid, stirring while adding, cross colloid mill 2- after mixing
3 times, take off bubble, obtain content stand-by；
(3) change glue：First 70 DEG C will be heated in 30 parts of capsule shells beeswaxs, 30 parts of glycerine putting into glue tanks, in rotating speed be
By 40 parts of gelatin being slowly added to glue tanks under the stirring of 40rpm, and continue to be heated to 80 DEG C, stirring boils 0.5 hour, take off
Obtain glue after bubble, 100 mesh filter, place 6 hours at 50 DEG C, standby；
(4) pressure watt：Start soft capsule press, carry out suppressing capsule and pill, production environment temperature 18-22 DEG C, relative humidity 40-
60%, control content thing 0.3g/ grain, the capsule and pill tape transport suppressing is shaped 5 hours in rotating cage；
(5) ball, drying are washed：It is carried out capsule and pill with the edible alcohol that volumetric concentration is 83%, until capsule and pill any surface finish,
Bright, no grease, then capsule and pill is gone to hothouse and be dried, control and room temperature 30 DEG C is dried, relative humidity 28%, it is dried 45
Hour, soft capsule is loaded in bottle, 60/bottle.
Embodiment 7
In order to prove simple addition EGCG in natto product, and add after median chain triglyceride oil oil soluble
The effect of EGCG, wherein by mixing that Bacillus natto, Nattokinase, sunflower oil, content beeswax and fatty acid glyceride are mixed to get
Compound, hereinafter referred to as natto essence, the natto essence in this test specimen to be prepared using the formula ratio in embodiment 5,
Then pass through the preparation method of the bean-containing capsule in any one of embodiment 1- embodiment 6, prepare capsule material；
Just 30 wistar rats are randomly divided into 5 groups, and every group 6, each group is respectively：
1st, blank group (Control), gavages the same number of capsulae vacuus with administration group.
2nd, natto " Jinghuasu " essence capsule group (hereinafter referred to as sample 1), dosage is 10kIU kg-1.
3rd, natto essence+EGCG Capsules group (hereinafter referred to as sample 2), dosage is 10kIU kg-1.
4th, natto essence+EGCG+ median chain triglyceride oil Capsules group (hereinafter referred to as sample 3), dosage is 10kIU
kg-1.
Above-mentioned natto essence is prepared using the component of embodiment 5 and proportioning, contains 3.5 parts in parts by weight and receives
Beans bacterium, 10 parts of Nattokinases, 70 portions of sunflower oils, 5 parts of content beeswaxs and 3.5 parts of fatty acid glycerides, and its capsule shells is with weight
, containing 15 parts of capsule shells beeswaxs, 60 parts of gelatin and 25 parts of glycerine, sample 1 is consistent with the EGCG content in sample 2, with weight for amount part meter
Amount part meter all EGCG containing 1 part.
It is given daily 1 time, continuous 7 days.The 8th day after administration, rat is carried out with mass percent for 3.5% chloraldurate
Intraperitoneal anesthesia (1ml/100g), operation separates bilateral common carotid arteries.Each one 1.2cm*3.0cm of pad under bilateral common carotid arteries
Plastic tab, with being impregnated with 50 μ l FeCl3The filter paper bar (1.0cm*1.5cm) of solution (10%, w/w) covers Bilateral Cervical and always moves
Arteries and veins.Take off filter paper bar after 20min, cut color change interval and cleaned with physiological saline, after filter paper suck dry moisture, measure its length, and
Use analysis level precise weighing.
The weight of unit length thrombus is designated as bolt weight (mg/cm) relatively, and calculates anti-bolt rate (%) with following formula.
/ W the comparison × 100% of anti-bolt rate %=(W comparison-W administration)
W comparison-control group average relative bolt weight；W administration-administration group average relative bolt weight.
Calculate anti-bolt rate by above formula, and statistical analysis are carried out to result, the results are shown in Table 1.
Table 1 different dosing group anti-bolt rate result
| Group | Bolt weight (mg cm-1) | Anti- bolt rate (%) |
| Blank control group | 5.63±1.10 | / |
| Sample 1 | 2.33±0.76 | 58.61 |
| Sample 2 | 0.94±0.32 | 83.30 |
| Sample 3 | 3.62±1.01 | 35.70 |
Result shows, by contrasting with sample 3 to blank control group, illustrates that natto essence has significantly anti-bolt
Effect (P<0.05), the anti-bolt effect of sample 1 and sample 2 is all significantly stronger than sample 3, illustrates that the addition of EGCG can strengthen natto
The anti-bolt effect of product；And the anti-bolt effect of sample 2 is significantly higher than sample 1, illustrates to dissolve by median chain triglyceride oil again
To EGCG have and than with EGCG, there is preferably anti-bolt effect merely.
It follows that improving the EGCG that the material absorbing is dissolved in median chain triglyceride oil, this is because EGCG is by middle chain
Triglyceride wraps, and not only ensure that the meltage of EGCG, also efficiently avoid the problem of EGCG oxidation by air, make
The effect obtaining EGCG can sufficiently be used.
Embodiment 8 EGCG improves the stability of composition system
According to the compound method of embodiment 7, prepare sample 1, sample 2 and sample 3, then sample 1, sample 2 and sample 3
It is respectively placed in 37 DEG C of environment, after 30 days, measures the enzymatic activity of each sample with fibrin plate method, calculate remnant enzyme activity
Power.
The residual enzyme activity situation of table 2 sample 1, sample 2 and sample 3
| Group | Residual enzyme activity |
| Sample 1 | 53.6% |
| Sample 2 | 82% |
| Sample 3 | 28.9% |
Result：After the composition powder of sample 1, sample 2 and sample 3 is placed 30 days under 37 DEG C of environment, residual enzyme activity
It is respectively 53.6%, 82% and 28.9%, illustrate that bean-containing capsule improves the temperature stability of NK.
More than, the only present invention preferably specific embodiment, but the protection domain of invention is not limited thereto, and any ripe
Know those skilled in the art the invention discloses technical scope in, the change or replacement that can readily occur in, all should contain
Cover within protection scope of the present invention.Therefore, protection scope of the present invention should be defined by the protection domain of claims.
Claims (8)
1. a kind of bean-containing capsule, be made up of capsule shells and the content that is located in described capsule shells it is characterised in that：Described interior
Tolerant contain following component in parts by weight：Median chain triglyceride oil 3-8 part, EGCG0.1-0.5 part, Bacillus natto 1-4 part, natto
Kinases 10-30 part, sunflower oil 45-75 part, content beeswax 3-10 part and fatty acid glyceride 3-10 part, described capsule shells with
Weight portion meter contains following component：Gelatin 40-70 part, glycerine 15-30 part and capsule shells beeswax 10-30 part.
2. bean-containing capsule according to claim 1 is it is characterised in that the content of described EGCG is not less than 98%.
3. bean-containing capsule according to claim 1 and 2 is it is characterised in that the activity of described Nattokinase is not less than
1200FU.
4. the bean-containing capsule according to any one of claim 1-3 is it is characterised in that described content contains in parts by weight
There is following component：5 parts of median chain triglyceride oil, 0.3 part of EGCG, 3 parts of Bacillus natto, 20 parts of Nattokinase, 60 parts of sunflower oil, interior
3.7 parts of tolerant beeswax and 8 parts of fatty acid glyceride, described capsule shells contain following component in parts by weight：45 parts of gelatin, sweet
Oily 30 parts and 25 parts of capsule shells beeswax.
5. the bean-containing capsule according to any one of claim 1-3 is it is characterised in that described content contains in parts by weight
There is following component：6.28 parts of median chain triglyceride oil, 0.42 part of EGCG, 4 parts of Bacillus natto, 25 parts of Nattokinase, sunflower oil 45
Part, 9.3 parts of content beeswax and 10 parts of fatty acid glyceride, described capsule shells contain following component in parts by weight：Gelatin
70 parts, 20 parts of glycerine and 10 parts of capsule shells beeswax.
6. the bean-containing capsule according to any one of claim 1-3 is it is characterised in that described content contains in parts by weight
There is following component：5.8 parts of median chain triglyceride oil, 0.35 part of EGCG, 3.2 parts of Bacillus natto, 15 parts of Nattokinase, sunflower oil 65,
6 parts of content beeswax and fatty acid glyceride 4.65, described capsule shells contain following component in parts by weight：40 parts of gelatin,
30 parts of glycerine and 30 parts of capsule shells beeswax.
7. a kind of preparation method of the bean-containing capsule according to any one of claim 1-6 it is characterised in that：The method bag
Include following steps：
(1) weigh：Weigh the median chain triglyceride oil of formula ratio, EGCG, Bacillus natto, Nattokinase, sunflower oil, content honeybee
Wax, capsule shells beeswax, fatty acid glyceride, gelatin and glycerine, standby；
(2) dispensing：Bacillus natto, Nattokinase, sunflower oil and fatty acid glyceride are mixed, obtains mixture；
Median chain triglyceride oil is heated to when 60-90 DEG C and adds content beeswax and EGCG, stirring melting 0.5-1 hour, obtain
To oleosol, then it is cooled to 15-30 DEG C of insulation, stand-by；
Above-mentioned mixture is added in oleosol, stirring while adding, cross colloid mill 2-3 time after mixing, take off bubble, obtain
Content is stand-by；
(3) change glue：First 50-70 DEG C will be heated in capsule shells beeswax, glycerine putting into glue tank, in rotating speed stirring for 15-45rpm
Mix and under state, gelatin is slowly added to, and continue to be heated to 80-100 DEG C, stirring boils 0.5-1 hour, obtains glue after de-bubbled
Liquid, and the filtration of 80-120 mesh, place 4-6 hour at 40-60 DEG C, standby；
(4) pressure watt：Start soft capsule press, carry out suppressing capsule and pill, control temperature and the relative humidity of production environment, suppress
Capsule and pill tape transport shape in rotating cage 3-5 hour；
(5) ball, drying are washed：It is carried out capsule and pill with the edible alcohol that volumetric concentration is 15-95%, until capsule and pill any surface finish,
Bright, no grease, then capsule and pill is gone to hothouse and be dried, control and room temperature is dried for 24-30 DEG C, relative humidity 20-
30%, 24-48 hour is dried, obtains soft capsule, soft capsule is loaded in bottle.
8. the preparation method of bean-containing capsule according to claim 7 is it is characterised in that in step (4), described production environment
Temperature is 18-22 DEG C, and relative humidity is 40-60%, and the loading amount of content is 0.3g/ grain.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109588671A (en) * | 2018-11-16 | 2019-04-09 | 江苏大学 | A kind of Nattokinase bee-glue composition and preparation method thereof for treating fatty liver |
| JP2019118341A (en) * | 2017-12-28 | 2019-07-22 | 小林製薬株式会社 | Food product containing polyphenol |
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| US20060141095A1 (en) * | 2004-12-28 | 2006-06-29 | Japan Bio Science Laboratory Co., Ltd. | Method for producing foods from culture of Bacillus natto |
| CN101117630A (en) * | 2006-07-31 | 2008-02-06 | 北京小西保健食品有限公司 | Preparation method of nattokinase powder and natto Brazil mushroom capsule |
| CN101214263A (en) * | 2007-12-26 | 2008-07-09 | 浙江大学宁波理工学院 | Method for preparing freeze-dried powder containing bacillus natto and nattokinase |
| CN102138660A (en) * | 2010-02-02 | 2011-08-03 | 梁刚 | Natto capsules and preparation method thereof |
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| US20060141095A1 (en) * | 2004-12-28 | 2006-06-29 | Japan Bio Science Laboratory Co., Ltd. | Method for producing foods from culture of Bacillus natto |
| CN101117630A (en) * | 2006-07-31 | 2008-02-06 | 北京小西保健食品有限公司 | Preparation method of nattokinase powder and natto Brazil mushroom capsule |
| CN101214263A (en) * | 2007-12-26 | 2008-07-09 | 浙江大学宁波理工学院 | Method for preparing freeze-dried powder containing bacillus natto and nattokinase |
| CN102138660A (en) * | 2010-02-02 | 2011-08-03 | 梁刚 | Natto capsules and preparation method thereof |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2019118341A (en) * | 2017-12-28 | 2019-07-22 | 小林製薬株式会社 | Food product containing polyphenol |
| CN111479474A (en) * | 2017-12-28 | 2020-07-31 | 小林制药株式会社 | Food containing polyphenol |
| JP7423179B2 (en) | 2017-12-28 | 2024-01-29 | 小林製薬株式会社 | Foods containing polyphenols |
| CN109588671A (en) * | 2018-11-16 | 2019-04-09 | 江苏大学 | A kind of Nattokinase bee-glue composition and preparation method thereof for treating fatty liver |
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