CN106420574A - Propolis-type plant antibacterial mouth wash and preparation method thereof - Google Patents

Propolis-type plant antibacterial mouth wash and preparation method thereof Download PDF

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Publication number
CN106420574A
CN106420574A CN201611012377.4A CN201611012377A CN106420574A CN 106420574 A CN106420574 A CN 106420574A CN 201611012377 A CN201611012377 A CN 201611012377A CN 106420574 A CN106420574 A CN 106420574A
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propolis
extract
type plants
herbal medicine
chinese herbal
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CN106420574B (en
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陈亚飞
余汉谋
许静
姜兴涛
吴新星
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Botton Spices Co ltd
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Dongguan Bowden Spices Co Ltd
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    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
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    • A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
    • A61K31/519—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim ortho- or peri-condensed with heterocyclic rings
    • A61K31/525—Isoalloxazines, e.g. riboflavins, vitamin B2
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    • A61K8/67—Vitamins
    • A61K8/673—Vitamin B group
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    • A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
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    • A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
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Abstract

The invention relates to the technical field of mouth washes, particularly a propolis-type plant antibacterial mouth wash and a preparation method thereof. The propolis-type plant antibacterial mouth wash comprises the following raw materials in percentage by mass: 2-5% of supercritical propolis extract, 15-25% of Chinese herbal medicine extract, 0.3-0.5% of essential oil mixture, 10-20% of edible ethanol, 4-8% of synergist vitamin complex and the balance of deionized water. The preparation method comprises the following steps: uniformly mixing the composite essential oil and propolis extract with the edible ethanol to obtain a mixture A; adding the vitamin complex into the deionized water, adding the Chinese herbal medicine extract, and stirring uniformly to obtain a mixture B; and mixing the mixtures A and B, adding the deionized water, and stirring uniformly to obtain the mouth wash.

Description

A kind of propolis type plants antimicrobial mouthwash and preparation method thereof
Technical field
The present invention relates to mouthwash technical field is and in particular to a kind of propolis type plants antimicrobial mouthwash and its preparation side Method.
Background technology
With era development, oral health become the World Health Organization evaluate level of human health important indicator it One.Most of mouth disease and oral problem, microbial mainly due to causing a disease present in oral cavity.Oral bacteria picked-up sugar Produced acid can result in decayed tooth afterwards；Toxin produced by oral bacteria, may result in bleeding gums；Additionally, these bacteriums Metabolic waste can also result in calculus dentalis and halitosis etc..Therefore, control the main task that oral cavity pathogen is prevention mouth disease. The traditional Chinese medical science has the saying it is believed that oral disease is in retention of heat in the spleen and stomach or internal heat of " internal heat in send out, above for aphthous stomatitis " to the morbidity of oral disease Scorching or asthenic fire floating upward and send out.
Mouthwash is a kind of new oral health care product.Its convenient, fast, fashion, can make holding oral cleaning, disappear Removing abnormal flavor in oral cavity, takees good care of oral health, reduces interpersonal estrangement, promotes intimate sense.
Antimicrobial form mouthwash on the market uses chemical antiseptic, easy allergy mostly at present, and effect is single, for a long time Produce drug resistance using meeting also has certain impact, such as tooth discoloration to the oral cavity of people, and oral cavity becomes dry etc..
Content of the invention
In order to overcome shortcoming and defect present in prior art, it is an object of the invention to provide a kind of propolis type plant Antibacterial mouthwash, in the urgent need to a kind of new antibacterial mouthwash, not only pure natural, hypo-allergenic, have no side effect, fragrance pure and fresh solely Spy, and there is the effects such as anti-inflammation, hemostatic analgesia, clearing heat-fire.
Another goal of the invention of the present invention is to provide a kind of preparation method of propolis type plants antimicrobial mouthwash, and it has Preparation method is simple, it is easy to accomplish the feature of industrialized production.
The purpose of the present invention is achieved through the following technical solutions：
A kind of propolis type plants antimicrobial mouthwash, including the raw material of following mass percent：
Supercritical bee-glue extract 2-5%
Chinese herbal medicine extract 15-25%
Blend of essential oils 0.3-0.5%
Edible ethanol 10-20%
Synergist B B-complex 4-8%
Deionized water surplus；
Wherein, described supercritical bee-glue extract is obtained by following methods：
Choose propolis and be placed in zubzero temperature freezing 40-50h, then propolis is pulverized, put in High-pressure supercritical extraction equipment, be passed through CO2, add the ethanol that concentration is 50%-100% as entrainer, extraction temperature is 38-65 DEG C, extracting pressure is 20-40Mpa, Separation temperature is 30-60 DEG C, and separating pressure is 4-8Mpa, CO2Flow be 10-25L/h；First static extracting 40-60min, then Dynamic extraction 1-2h, collects extraction product and is evaporated to through 40-60 DEG C solvent-free, obtain final product supercritical bee-glue extract；
Wherein, described Chinese herbal medicine extract is obtained by following methods：
The mixture taking dark plum, cogongrass rhizome, Chinese gall, Chinese scholartree branch, almond, malabar nut is pulverized, plus alcohol solution dipping 1.5-2.5h Ebuillition of heated persistently decoction 70-90min afterwards, are filtered to remove filter residue, collect filtrate and are concentrated to give concentrate, concentrate quality is 1.5-2.5 times of raw material quality, stands 22-26h after adding fining agent stirring 5-10min, is then centrifuged for taking supernatant to add Enter propane diols, after stirring and dissolving, be Chinese herbal medicine extract, wherein, the mass concentration of described ethanol solution is 45-55%, described The addition of ethanol solution is 5 times amount of mixture quality, and the addition of described fining agent is the 0.5-2% of concentrate quality, institute The addition stating propane diols is the 40-50% of concentrate quality.
Supercritical extract mainly has antibacterial, antiviral, anti-inflammatory, antiulcer effect and gives the pure and fresh uniqueness of product Propolis fragrance.Each component in Chinese herbal medicine extract reaches the mesh of antibacterial, anti-inflammatory, hemostasis, clearing heat-fire by different mechanism 's.Essential oil compound can preferably penetrate into the inside of plaque bio-film, have significant killing action to oral bacteria.Increase Effect agent B B-complex assists to promote each point preferably to act on, and is beneficial to oral health.
Prepare propolis extract using supercritical extraction, this extracting process is green, the modernization abstraction technique of environmental protection. Gentle extraction conditions can keep active ingredient and fragrant ingredient activity in propolis to greatest extent, and product colour is than former Material is more shallow, does not contain the heavy metal in raw material and easy allergic component.
Propolis, bitter, acrid, cold.Returns spleen, stomach.Qi-restoratives for oral administration is weak, change turbid fat, only quench one's thirst；External application removing toxicity for detumescence.Inside take In body void early ageing, hyperlipidemia, quench one's thirst；Control outward for chapped skin, burn and scald.Propolis described in TCM Literature has moisturizing to give birth to The effects such as flesh, removing toxic substances desinsection, wind-expelling pain-stopping and treatment wind carious pain.Such as wave containing several functions composition in propolis extract Hair oil, flavonoids, phenolic acid class.In propolis extract, functional components such as flavones and phenolic acid class etc. have antibacterial, anti-inflammatory, suppressed Quick reaction, pain relieving, deodorant, promotion organization palingenesis.Volatile oil in propolis has sterilization and anticorrosion, anti-oxidant, antiviral, anti-swollen The widely biologically active such as knurl, and give product pure and fresh unique fragrance, make one body and mind pleasure.
Dark plum in Chinese herbal medicine extract：Taste is sour, puckery, mild-natured.Return liver, spleen, lung, large intestine channel.Have and astringe the lung, puckery intestines, promote the production of body fluid, The effect of claming ascaris.Modern study shows, smoked plum extractive can suppress streptococcus mitis, Streptococcus sanguis, Streptococcus mutans, gum porphin The activity of 4 kinds of oral cavity common bacteria of quinoline monad.
Chinese gall：Taste is sour, puckery, cold in nature.Return lung, large intestine, kidney channel.Astringe the lung fall fire, relieving diarrhea with astringents, arrest sweating, hemostasis, hygroscopic is held back Sore.Tannin class in nutgall extractive can suppress cariogenic bacteria (streptococcus mutans, Streptococcus sanguis, streptococcus salivarius, Actinomyces naeslundii, actinomyces viscosus, Bacillus acidi lactici) growth.
Cogongrass rhizome：Sweet, cold in nature.Return lung, stomach, bladder warp.Cool blood, hemostasis, reducing fever and causing diuresis.Huang in cogongrass rhizome extractive Ketone has anti-inflammation hemostasia, antibacterial preventing decayed tooth, the effect except halitosis.
Chinese scholartree branch：Bitter, mild-natured；The heart, Liver Channel.Dissipate stasis of blood hemostasis；Heat-clearing and damp-drying drug；Wind dispelling insecticide.Main uterine bleeding；Leukorrhea with reddish discharge；Hemorrhoid Sore；Scrotum is wet to itch；Pained；Hot eyes；Mange.Main chemical compositions in Chinese scholartree branch extract are the flavone compounds such as rutin, tool There are antibacterial, anti-inflammatory, antiulcer, antiallergy, the function such as anti-oxidant.
Almond, bitter, slightly warm in nature；Return lung, large intestine channel.Sending down abnormally ascending is relieving cough and asthma, relaxes bowel.For cough and asthma, fullness sensation in chest Abundant expectoration, deficiency of blood Tianjin is withered, dry constipation of intestines.Almond main active is amarogentin, has antiulcer, adjusts immunologic function, stops The cloperastine effect of breathing heavily etc..
Malabar nut：Bitter, pungent, temperature.Expelling wind and activating blood flow, blood stasis removing analgesic, synthetism.For fracturing, sprain, rheumatic arthritis, pain in the back. Active component adhatodine in extract etc. can significantly reduce dental plaque index and amount of bleeding.
Folium isatidis：Bitter, cold in nature.Return liver, the heart, stomach, the spleen channel.Extract has clearing heat and detoxicating, blood cooling and ecchymoses removing effect.
Multiple effective efficiency compositions are contained, above-mentioned formula is used in conjunction with, mutually coordinated, by having in extracts of Chinese herbal medicine Effect composition flavonoids, the sweet class of soap, the functional component such as alkaloids, tannin class plays a role, reach anti-inflammation, hemostatic analgesia, Effect of clearing heat-fire, effectively takees good care of oral health.
Wherein, in the preparation method of described supercritical bee-glue extract, the cryogenic temperature of supercritical bee-glue extract is -18 ℃.
Wherein, the amount of alcohol added in the preparation method of described supercritical bee-glue extract is the 10- of raw material gross mass 30%.
Wherein, in described Chinese herbal medicine extract preparation method, described fining agent is the mixing of chitosan quaternary ammonium salt and water Thing, in described fining agent, the mass concentration of chitosan quaternary ammonium salt is 1%.Chinese herbal medicine through too low alcohol extracting process, a lot of macromoleculars As pectin, starch, cellulose, pigment etc. also can bring product in the lump into.Chitosan quaternary ammonium salt belongs to a class and extracts from marine organisms Natural clarifying agent out.These macromolecular substances can effectively be removed by this fining agent, and the activity required for only retaining Composition, simultaneously clarification steps also ensure that the color of Chinese herbal medicine extract is unlikely to too deep and affects outward appearance.
Further, the dark plum in described Chinese herbal medicine extract, cogongrass rhizome, Chinese gall, Chinese scholartree branch, almond, the matter of malabar nut Amount ratio is 3：1-3：1-3：1-3：1-2：1.
Wherein, the pulverizing mesh number of described mixture is 200 mesh.
Wherein, the centrifugal condition in the preparation method of described Chinese herbal medicine extract is 5000-8000rpm, and centrifugation time is 20-30min.
Wherein, described blend of essential oils by Thymol and presses leaf oil according to mass ratio 3-7:7-3 mixes.
In described blend of essential oils, Thymol has sterilization, fungicidal action to oral cavity mucosa, and carious tooth chamber is had Anti-corrosion, local anesthetic action, for the disinfection in oral cavity, throat.By leaf oil, there is bactericidal action, the tax that also can make toothpaste etc. is fragrant Agent.
Wherein, described B B-complex by vitamin C, vitamin E, vitamin B12, four kinds of vitamin B2 according to mass ratio For 1-4：1-4：1-3：1-3 mixes.
In described B B-complex, vitamin C is highly important to periodontal, and shortage can cause periodontosis.Vitamin E pair Treatment mouth infection ulcer effect is notable, has antioxidation.Vitamin B12 is that DNA in people's tumor growth and repairs Propeller, and DNA then makes the generation of protein have reparation and therapeutic action to mucous membrane, canker sore is played with good prevention and makees With.Easily quarrel during Vitamin B2 deficiency inflammation, glossitis, cheilitis etc., the one-tenth that vitamin B2 can reduce canker sore produces.
The major function that Four Vitamins are had nothing in common with each other, compound use can be mutually coordinated, is complementary to one another, and can play more preferably Effect.
A kind of preparation method of propolis type plants antimicrobial mouthwash, composite essential oil and propolis extract are added edible ethanol In mix, obtain mixture A；B B-complex is added in deionized water, adds Chinese herbal medicine extract, stir, Obtain mixture B；By mixture A, B mix, plus deionized water stir the present invention propolis type plants antimicrobial mouthwash.
In the described preparation method of propolis type plants antimicrobial mouthwash, first by preferable for oil-soluble material in edible ethanol Middle dissolving, mixing.Water-soluble preferably material, such as extracts species, is dissolved in deionized water, then mixes both again, Good mixing effect.
The beneficial effects of the present invention is：Supercritical extract mainly has antibacterial, antiviral, anti-inflammatory, antiulcer work With and give product pure and fresh unique propolis fragrance.Each component in Chinese herbal medicine extract by different mechanism reach antibacterial, Anti-inflammatory, hemostasis, the purpose of clearing heat-fire.Essential oil compound can preferably penetrate into the inside of plaque bio-film, thin to oral cavity Bacterium has significant killing action.Synergist B B-complex assists to promote each point preferably to act on, and is beneficial to oral health.
The present invention, with natural plants, Chinese herbal medicine as raw material, focuses on the combination of natural products and modern advanced extractive technique, root According to the cause of disease of traditional Chinese medicine principle and oral disease, select the formula being suitable for the most.The mouthwash of the present invention is pure natural, hypo-allergenic, Have no side effect, the pure and fresh uniqueness of fragrance；Each active ingredient can be mutually coordinated, has the work(such as anti-inflammation, hemostatic analgesia, clearing heat-fire Effect.The method of the present invention is simple to operation, and industrialized production is strong, and preparation cost is low, and the active ingredient protectiveness of raw material is good.
Specific embodiment
For the ease of the understanding of those skilled in the art, with reference to embodiment, the present invention is further illustrated, real The content that the mode of applying refers to not limitation of the invention.
Embodiment 1
A kind of preparation method of supercritical bee-glue extract：
Choose the propolis 100g of high-quality, freeze 48h in -18 DEG C, then propolis is pulverized, put in High-pressure supercritical extraction equipment, It is passed through CO2, the ethanol adding 20g 60%, as entrainer, arranges extraction temperature, pressure is 60 DEG C, 20Mpa, separation temperature pressure Power is 50 DEG C, 5.5Mpa, CO2Flow be 10L/h.After first static extracting 50min, subsequent dynamic extraction 2 hours, collect extraction Take product, more solvent-free through 45 DEG C be evaporated to, obtain final product 10g supercritical bee-glue extract.
A kind of preparation method of Chinese herbal medicine extract：
The common 100g of six kinds of mixtures in dark plum, cogongrass rhizome, Chinese gall, Chinese scholartree branch, almond, malabar nut is followed successively by according to mass ratio 3：1：2：2：1：1 co-grinding to 200 mesh, plus 500g 50% alcohol solution dipping after 2 hours ebuillition of heated and continue 90min decoct Boil, be filtered to remove filter residue, collect filtrate and be concentrated into 200g.Add 3g fining agent, fining agent is chitosan quaternary ammonium salt, quality is dense Spend for 1%；After stirring 5min under normal temperature, stand 24h, 4000rpm centrifugation 20min after clarifying treatment, supernatant adds 81g's Propane diols, is 260g Chinese herbal medicine extract after stirring and dissolving.
A kind of preparation method of blend of essential oils：
Prepare the blend of essential oils of 100g, each constituent mass ratio is：Thymol 60%, press leaf oil 40%.
A kind of B B-complex, is grouped into by the group of following mass fraction：
Prepare the B B-complex of 100g, each constituent mass ratio is：Vitamin C 30%, vitamin E 30%, vitamin B12 20%th, Cobastab 20%.
A kind of propolis type plants antimicrobial mouthwash, is grouped into by the group of following mass fraction：
Supercritical bee-glue extract 3%, Chinese herbal medicine extract 15%, blend of essential oils 0.3%, edible ethanol 12%, B B-complex 4.5%th, deionized water surplus.
A kind of preparation method of propolis type plants antimicrobial mouthwash, composite essential oil and propolis extract are added edible ethanol In mix, obtain mixture A；B B-complex is added in deionized water, adds Chinese herbal medicine extract, stir, Obtain mixture B；By mixture A, B mix, plus deionized water stir the present invention propolis type plants antimicrobial mouthwash.
Embodiment 2
The present invention is with the difference of embodiment 1：A kind of propolis type plants antimicrobial mouthwash, by the group packet of following mass fraction Become：
Supercritical bee-glue extract 2%, Chinese herbal medicine extract 25%, blend of essential oils 0.35%, edible ethanol 18%, B B-complex 6%th, deionized water surplus.
Embodiment 3
The present invention is with the difference of embodiment 1：A kind of propolis type plants antimicrobial mouthwash, by the group packet of following mass fraction Become：
Supercritical bee-glue extract 5%, Chinese herbal medicine extract 23%, blend of essential oils 0.5%, edible ethanol 15%, B B-complex 7%th, deionized water surplus.
Embodiment 4:
The present invention is with the difference of embodiment 1：A kind of propolis type plants antimicrobial mouthwash, by the group packet of following mass fraction Become：
Supercritical bee-glue extract 4%, Chinese herbal medicine extract 20%, blend of essential oils 0.4%, edible ethanol 16%, B B-complex 6%th, deionized water surplus.
The antibacterial test of propolis type plants antimicrobial mouthwash
The antibacterial effect of the propolis type plants antimicrobial mouthwash that test aforementioned four embodiment prepares.Bacterial strain often adopts four kinds The oral cavity pathogen seen：Streptococcus mitis, Streptococcus sanguis, Streptococcus mutans, porphyromonas gingivalis.
Method of testing：
Minimal inhibitory concentration（MIC）Measure：Measured using agar dilution, the minimum extract concentrations of complete inhibition bacterial growth For extract to the MIC MIC for examination bacterium.Experimental result is shown in Table 1.
Table 1：The minimal inhibitory concentration of propolis type plants antimicrobial mouthwash（MIC, %）
Streptococcus mutans Porphyromonas gingivalis Streptococcus sanguis Streptococcus mitis
Embodiment 1 0.165 0.125 0.249 0.267
Embodiment 2 0.157 0.113 0.225 0.301
Embodiment 3 0.139 0.136 0.268 0.287
Embodiment 4 0.129 0.109 0.212 0.238
Table 1 result shows, 4 kinds of embodiments have preferable inhibition for four kinds of common oral cavity pathogens, and embodiment 4 Inhibition best.
The good result of above-mentioned mouthwash is described by tests below：
Research object：Choose and there is the volunteer of the symptoms such as decayed tooth, bacterial plaque, carious tooth inflammation, bleeding gums, canker sore totally 200 People, men and women half and half, one full year of life at age 18-60, average age 30 one full year of life, is randomly divided into 5 groups, every group of 40 people.
Use one bottle of about 2500g of mouthwash that the proportioning of embodiment 5-8 is obtained respectively, daily breakfast, lunch and dinner after meal and are slept first 4 times and made Mouthwash with the present invention.Using method：First mouthwash containing a bite, remains silent;Then agitate two cheeks and lip, allow mouthwash with Tooth is fully contacted, and repeatedly rinses each position in oral cavity using the impulse force of water, spues in a moment, is repeated 2 times, and makes mouthwash by tooth The swill at the positions such as seam, gum, sublingual and tongue labial groove is rinsed well.Gargle every time 3 minutes about, repeat above behaviour every time Make.It is efficient that test counts it after carrying out 60 days（%）.Criterion of therapeutical effect：（1）It is obviously improved：Subjective symptoms has and significantly subtracts Light or disappear.（2）Alleviate：Subjective symptoms has a certain degree of mitigation but less obvious.（3）Invalid：Oral conditions still substantially or Do not mitigate.（4）Deteriorate：Oral conditions become worse.Result of the test is shown in Table 2：
Table 2：Using effect in target group for the propolis type plants antimicrobial mouthwash
Embodiment 1 Embodiment 2 Embodiment 3 Embodiment 4
It is obviously improved 21 19 20 22
Alleviate 16 17 16 16
Invalid 3 4 4 2
Deteriorate 0 0 0 0
Total effective rate 92.50% 90% 90% 95%
Table 2 result shows：For the personage with symptoms such as decayed tooth, bacterial plaque, carious tooth inflammation, bleeding gums, canker sore, for a long time Using the mouthwash of the present invention, all there is preferable effect, and embodiment 4 effect is better than other embodiment.
Above-mentioned a kind of propolis type plants antimicrobial mouthwash and preparation method thereof, with pure natural and have peculiar fragrance and antibacterial The supercritical bee-glue of effect and Chinese herbal medicine extract are active ingredient, be combined certain essential oil and natural complex is made, be resistant to Bacterium anti-inflammatory, hemostatic analgesia, clearing heat-fire etc..Using rear no allergic reaction and have no toxic side effect, and effect is good, simple and convenient, Men and women, old and young all can use.
Above-described embodiment is the present invention preferably implementation, and in addition, the present invention can be realized with alternate manner, Without departing from obvious replacement any on the premise of present inventive concept all within protection scope of the present invention.

Claims (10)

1. a kind of propolis type plants antimicrobial mouthwash it is characterised in that：Raw material including following mass percent：
Supercritical bee-glue extract 2-5%
Chinese herbal medicine extract 15-25%
Blend of essential oils 0.3-0.5%
Edible ethanol 10-20%
Synergist B B-complex 4-8%
Deionized water surplus；
Wherein, described supercritical bee-glue extract is obtained by following methods：
Choose propolis and be placed in zubzero temperature freezing 40-50h, then propolis is pulverized, put in High-pressure supercritical extraction equipment, be passed through CO2, add the ethanol that concentration is 50%-100% as entrainer, extraction temperature is 38-65 DEG C, extracting pressure is 20-40Mpa, Separation temperature is 30-60 DEG C, and separating pressure is 4-8Mpa, CO2Flow be 10-25L/h；First static extracting 40-60min, then Dynamic extraction 1-2h, collects extraction product and is evaporated to through 40-60 DEG C solvent-free, obtain final product supercritical bee-glue extract；
Wherein, described Chinese herbal medicine extract is obtained by following methods：
The mixture taking dark plum, cogongrass rhizome, Chinese gall, Chinese scholartree branch, almond, malabar nut is pulverized, plus alcohol solution dipping 1.5-2.5h Ebuillition of heated persistently decoction 70-90min afterwards, are filtered to remove filter residue, collect filtrate and are concentrated to give concentrate, concentrate quality is 1.5-2.5 times of raw material quality, stands 22-26h after adding fining agent stirring 5-10min, is then centrifuged for taking supernatant to add Enter propane diols, after stirring and dissolving, be Chinese herbal medicine extract, wherein, the mass concentration of described ethanol solution is 45-55%, described The addition of ethanol solution is 5 times amount of mixture quality, and the addition of described fining agent is the 0.5-2% of concentrate quality, institute The addition stating propane diols is the 40-50% of concentrate quality.
2. a kind of propolis type plants antimicrobial mouthwash according to claim 1 it is characterised in that：Described supercritical bee-glue carries Take in the preparation method of thing, the cryogenic temperature of supercritical bee-glue extract is -18 DEG C.
3. a kind of propolis type plants antimicrobial mouthwash according to claim 1 it is characterised in that：Described supercritical bee-glue carries Take the 10-30% that the amount of alcohol added in the preparation method of thing is raw material gross mass.
4. a kind of propolis type plants antimicrobial mouthwash according to claim 1 it is characterised in that：Described Chinese herbal medicine extracts In thing preparation method, described fining agent is the mixture of chitosan quaternary ammonium salt and water, chitosan quaternary ammonium salt in described fining agent Mass concentration is 1%.
5. a kind of propolis type plants antimicrobial mouthwash according to claim 1 it is characterised in that：Described Chinese herbal medicine extract In dark plum, cogongrass rhizome, Chinese gall, Chinese scholartree branch, almond, malabar nut mass ratio be 3：1-3：1-3：1-3：1-2：1.
6. a kind of propolis type plants antimicrobial mouthwash according to claim 5 it is characterised in that：The pulverizing of described mixture Mesh number is 200 mesh.
7. a kind of propolis type plants antimicrobial mouthwash according to claim 1 it is characterised in that：Described Chinese herbal medicine extract Preparation method in centrifugal condition be 5000-8000rpm, centrifugation time be 20-30min.
8. the propolis type plants antimicrobial mouthwash described in a kind of claim 1 according to claim 1 it is characterised in that： Described blend of essential oils by Thymol and presses leaf oil according to mass ratio 3-7:7-3 mixes.
9. the propolis type plants antimicrobial mouthwash described in a kind of claim 1 according to claim 1 it is characterised in that： Described B B-complex is 1-4 by vitamin C, vitamin E, vitamin B12, four kinds of vitamin B2 according to mass ratio：1-4：1- 3：1-3 mixes.
10. the propolis type plants antimicrobial mouthwash described in a kind of any one of claim 1-9 preparation method it is characterised in that： Composite essential oil and propolis extract are added in edible ethanol and mixes, obtain mixture A；B B-complex is added deionization In water, add Chinese herbal medicine extract, stir, obtain mixture B；Mixture A, B are mixed, plus deionized water stirs Obtain mouthwash.
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CN110522714A (en) * 2019-08-29 2019-12-03 哈尔滨医科大学 A kind of dark plum mouthwash effervescent tablet and preparation method thereof with rush salivary secretion effect

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CN110522714A (en) * 2019-08-29 2019-12-03 哈尔滨医科大学 A kind of dark plum mouthwash effervescent tablet and preparation method thereof with rush salivary secretion effect

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