CN106420380A - Preparation method of skin moistening water containing silk amino acid - Google Patents
Preparation method of skin moistening water containing silk amino acid Download PDFInfo
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- CN106420380A CN106420380A CN201611055968.XA CN201611055968A CN106420380A CN 106420380 A CN106420380 A CN 106420380A CN 201611055968 A CN201611055968 A CN 201611055968A CN 106420380 A CN106420380 A CN 106420380A
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- sodium carbonate
- cellulase
- aqueous sodium
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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Abstract
The invention provides a preparation method of skin moistening water containing silk amino acid. The skin moistening water is prepared by cleaning silk and placing in a sodium carbonate water solution to degum; after being cleaned by de-ionized water, obtaining silk fibroin; then adding the silk fibroin into a calcium chloride solution, and adjusting pH value to 5-6; adding papain and trypsin; heating and extracting, cooling, and delivering the extracted fluid into macroporous adsorption resin for absorption; collecting penetrated fluid; adjusting pH of the penetrated liquid to 8.0-10.0, guiding the penetrated liquid into an ultrafiltration membrane for filtering, depressurizing ultrafiltration penetrated liquid for concentration, and adding concentrate into glycerinum; finally, adding propylene glycol, citric acid, water, menthol, butanediol, Gul glue, sodium alginate and microcrystalline cellulose; mixing to obtain the skin moistening water. By taking the silk amino acid as an active ingredient, the prepared skin moistening water can effectively maintain skin moisture.
Description
Technical field
The invention belongs to technical field of skin care, and in particular to a kind of preparation side of the skin moisture-keeping water containing silkworm silk aminoacid
Method.
Background technology
Temperature change and pressure that air-conditioning, environmental pollution, Seasonal conversion bring, and skin aging, metabolism slow down etc. because
Element all can cause skin water loss, make skin coarse, dim mute, form dry stricture of vagina, cause many skin problems.Face is in drying
State one day, will lose original elasticity.Last till the 3rd day, tight, peeling, pigmentation are accelerated.Drying time exceedes
Seven days, mottle will be produced, deposit toxin.Once more than 30 days, various intractable mottles, wrinkle, skin aging speed will be generated
Degree is exceedingly fast.The water content on the top layer of our skins is very sufficient in general, it should be 10%～30%, if skin
The water content on skin top layer is less than 10%, and skin will be relatively dry, tight, or even decortication desquamation, and others looks also and can compare
Coarse, therefore must allow skin moisture-keeping and moisturizing.Accurately understand the skin condition of oneself, be assigned to the moisture of abundance and improve angle
The barrier function of matter layer, just can ensure that the healthy, young of skin, and skin moistening moisture-keeping cosmetics become a kind of and lead the leading of cosmetics
Direction.
Silkworm silk aminoacids complex is the ispol for extracting from natural silk, containing 18 kinds of aminoacid, wherein 8 kinds
Aminoacid is needed by human and itself can not manufacture, these aminoacid have higher physiologically active and human compatibility,
Easily it is absorbed by tissue, has significant health-care effect to human body.As Glycine and serine can reduce Blood Cholesterol concentration,
Reduce blood glucose value, prevent and treat hypertension and diabetes effect；Alanine can promote the metabolism of ethanol in blood, have Antialcoholic liver-protecting work(
Energy；L-Tyrosine promotes thyroxine and adrenergic synthesis, can prevent and treat senile dementia etc..Silkworm silk aminoacids complex is also one
Highly active beauty and skin care raw material is planted, is formed, promote skin tissue regeneration, prevent with moisturizing, nutrition skin, suppression melanin
The effect such as cracking and chemical damage, is widely used in cosmetics, is a kind of rare functional biological materials.
Content of the invention
The technical problem of solution：The invention provides a kind of preparation method of the skin moisture-keeping water containing silkworm silk aminoacid, institute
Obtain moisture retention water to have preferably to skin moisturizing, moisturizing.
Technical scheme：A kind of preparation method of the skin moisture-keeping water containing silkworm silk aminoacid, comprises the following steps：
Step 1, takes silkworm silk and cleans degumming in the 10-20wt.% aqueous sodium carbonate for be placed in 5-10 times of weight, through deionization
Fibroin albumen is obtained after water cleaning；
Step 2, fibroin albumen is added in the 10-15wt.% calcium chloride solution of 2-2.5 times of weight, adjusts pH to 5-6,
Addition 0.2-0.6wt.% papain and 0.1-0.4wt.% trypsin, heating extraction, cooling, obtain extracting solution；
Step 3, extracting solution feeding macroporous adsorbent resin is adsorbed, is collected permeate；
Step 4, adjusts the pH to 8.0-10.0 of permeate, imports ultrafilter membrane and is filtered, obtains ultrafiltration permeate；
Step 5, in parts by weight, ultrafiltration permeate concentrating under reduced pressure, gained concentrate 0.5-1.2 part is added to glycerol 3-8
In part, Propylene Glycol 4-8 part, citric acid 0.4-1.2 part, water 20-40 part, Mentholum 0.2-0.4 part, butanediol 0.5-1 is added
Part, guar gum 0.1-0.5 part, sodium alginate 0.2-0.5 part, Microcrystalline Cellulose 0.1-0.4 part, mixing, obtain final product.
Further, contain 0.2-0.5wt.% cellulase in the aqueous sodium carbonate of step 1.
Further, in step 1, de-alkali additives are 40-50 DEG C, 30-50min.
Further, in step 2, heating extraction condition is 30-40 DEG C, 30-40min.
Further, polystyrene macroporous adsorbent resin in step 3.
Further, the molecular cut off of the ultrafilter membrane used in step 4 be in 100000Da.
Beneficial effect：The present invention is using silkworm silk aminoacid as effective ingredient, and the skin moisture-keeping water for preparing can be effective
Ground maintains moisture of skin.
Specific embodiment
Embodiment 1
A kind of preparation method of the skin moisture-keeping water containing silkworm silk aminoacid, comprises the following steps：
Step 1, takes silkworm silk and cleans degumming in the 10wt.% aqueous sodium carbonate for be placed in 5 times of weight, cleans through deionized water
After obtain fibroin albumen；
Step 2, fibroin albumen is added in the 10wt.% calcium chloride solution of 2 times of weight, adjusts pH to 5, is added
0.2wt.% papain and 0.4wt.% trypsin, heating extraction, cooling, obtain extracting solution；
Step 3, extracting solution feeding macroporous adsorbent resin is adsorbed, is collected permeate；
Step 4, adjusts the pH to 8.0 of permeate, imports ultrafilter membrane and is filtered, obtains ultrafiltration permeate；
Step 5, in parts by weight, ultrafiltration permeate concentrating under reduced pressure, 0.5 part of gained concentrate is added in 3 parts of glycerol, then
Add 4 parts of Propylene Glycol, 0.4 part of citric acid, 20 parts of water, 0.2 part of Mentholum, 0.5 part of butanediol, 0.1 part of guar gum, sodium alginate
0.2 part, 0.1 part of Microcrystalline Cellulose, mixing, obtain final product.
Wherein, contain 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in；
In step 2, heating extraction condition is 30 DEG C, 40min；Polystyrene macroporous adsorbent resin in step 3；Ultrafiltration used in step 4
The molecular cut off of film be in 100000Da.
Embodiment 2
A kind of preparation method of the skin moisture-keeping water containing silkworm silk aminoacid, comprises the following steps：
Step 1, takes silkworm silk and cleans degumming in the 14wt.% aqueous sodium carbonate for be placed in 7 times of weight, cleans through deionized water
After obtain fibroin albumen；
Step 2, fibroin albumen is added in the 13wt.% calcium chloride solution of 2 times of weight, adjusts pH to 6, is added
0.3wt.% papain and 0.2wt.% trypsin, heating extraction, cooling, obtain extracting solution；
Step 3, extracting solution feeding macroporous adsorbent resin is adsorbed, is collected permeate；
Step 4, adjusts the pH to 9.0 of permeate, imports ultrafilter membrane and is filtered, obtains ultrafiltration permeate；
Step 5, in parts by weight, ultrafiltration permeate concentrating under reduced pressure, 0.7 part of gained concentrate is added in 6 parts of glycerol, then
Add 5 parts of Propylene Glycol, 0.7 part of citric acid, 23 parts of water, 0.3 part of Mentholum, 0.8 part of butanediol, 0.3 part of guar gum, sodium alginate
0.4 part, 0.2 part of Microcrystalline Cellulose, mixing, obtain final product.
Wherein, contain 0.3wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 43 DEG C, 42min 0.3wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 43 DEG C, 42min 0.3wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 43 DEG C, 42min 0.3wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 43 DEG C, 42min 0.3wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 43 DEG C, 42min 0.3wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 43 DEG C, 42min 0.3wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 43 DEG C, 42min 0.3wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 43 DEG C, 42min 0.3wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 43 DEG C, 42min 0.3wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 43 DEG C, 42min 0.3wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 43 DEG C, 42min 0.3wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 43 DEG C, 42min 0.3wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 43 DEG C, 42min 0.3wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 43 DEG C, 42min 0.3wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 43 DEG C, 42min 0.3wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 43 DEG C, 42min 0.3wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 43 DEG C, 42min 0.3wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 43 DEG C, 42min 0.3wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 43 DEG C, 42min 0.3wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 43 DEG C, 42min 0.3wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 43 DEG C, 42min 0.3wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 43 DEG C, 42min 0.3wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 43 DEG C, 42min 0.3wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 43 DEG C, 42min 0.3wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 43 DEG C, 42min 0.3wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 43 DEG C, 42min 0.3wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 43 DEG C, 42min 0.3wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 43 DEG C, 42min 0.3wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 43 DEG C, 42min 0.3wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 43 DEG C, 42min 0.3wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 43 DEG C, 42min 0.3wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 43 DEG C, 42min 0.3wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 43 DEG C, 42min 0.3wt.% cellulase in；
In step 2, heating extraction condition is 32 DEG C, 35min；Polystyrene macroporous adsorbent resin in step 3；Ultrafiltration used in step 4
The molecular cut off of film be in 100000Da.
Embodiment 3
A kind of preparation method of the skin moisture-keeping water containing silkworm silk aminoacid, comprises the following steps：
Step 1, takes silkworm silk and cleans degumming in the 18wt.% aqueous sodium carbonate for be placed in 8 times of weight, cleans through deionized water
After obtain fibroin albumen；
Step 2, fibroin albumen is added in the 14wt.% calcium chloride solution of 2.5 times of weight, adjusts pH to 6, is added
0.5wt.% papain and 0.2wt.% trypsin, heating extraction, cooling, obtain extracting solution；
Step 3, extracting solution feeding macroporous adsorbent resin is adsorbed, is collected permeate；
Step 4, adjusts the pH to 9.0 of permeate, imports ultrafilter membrane and is filtered, obtains ultrafiltration permeate；
Step 5, in parts by weight, ultrafiltration permeate concentrating under reduced pressure, 0.9 part of gained concentrate is added in 7 parts of glycerol, then
Add 6 parts of Propylene Glycol, 0.7 part of citric acid, 34 parts of water, 0.3 part of Mentholum, 0.8 part of butanediol, 0.4 part of guar gum, sodium alginate
0.3 part, 0.1 part of Microcrystalline Cellulose, mixing, obtain final product.
Wherein, contain 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 40 DEG C, 50min 0.2wt.% cellulase in；
In step 2, heating extraction condition is 30 DEG C, 40min；Polystyrene macroporous adsorbent resin in step 3；Ultrafiltration used in step 4
The molecular cut off of film be in 100000Da.
Embodiment 4
A kind of preparation method of the skin moisture-keeping water containing silkworm silk aminoacid, comprises the following steps：
Step 1, takes silkworm silk and cleans degumming in the 20wt.% aqueous sodium carbonate for be placed in 10 times of weight, clear through deionized water
Fibroin albumen is obtained after washing；
Step 2, fibroin albumen is added in the 15wt.% calcium chloride solution of 2.5 times of weight, adjusts pH to 6, is added
0.6wt.% papain and 0.1wt.% trypsin, heating extraction, cooling, obtain extracting solution；
Step 3, extracting solution feeding macroporous adsorbent resin is adsorbed, is collected permeate；
Step 4, adjusts the pH to 10.0 of permeate, imports ultrafilter membrane and is filtered, obtains ultrafiltration permeate；
Step 5, in parts by weight, ultrafiltration permeate concentrating under reduced pressure, 1.2 parts of gained concentrate is added in 8 parts of glycerol, then
Add 8 parts of Propylene Glycol, 1.2 parts of citric acid, 40 parts of water, 0.4 part of Mentholum, 1 part of butanediol, 0.5 part of guar gum, sodium alginate
0.5 part, 0.4 part of Microcrystalline Cellulose, mixing, obtain final product.
Wherein, contain 0.5wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 50 DEG C, 30min 0.5wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 50 DEG C, 30min 0.5wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 50 DEG C, 30min 0.5wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 50 DEG C, 30min 0.5wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 50 DEG C, 30min 0.5wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 50 DEG C, 30min 0.5wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 50 DEG C, 30min 0.5wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 50 DEG C, 30min 0.5wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 50 DEG C, 30min 0.5wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 50 DEG C, 30min 0.5wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 50 DEG C, 30min 0.5wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 50 DEG C, 30min 0.5wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 50 DEG C, 30min 0.5wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 50 DEG C, 30min 0.5wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 50 DEG C, 30min 0.5wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 50 DEG C, 30min 0.5wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 50 DEG C, 30min 0.5wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 50 DEG C, 30min 0.5wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 50 DEG C, 30min 0.5wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 50 DEG C, 30min 0.5wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 50 DEG C, 30min 0.5wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 50 DEG C, 30min 0.5wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 50 DEG C, 30min 0.5wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 50 DEG C, 30min 0.5wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 50 DEG C, 30min 0.5wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 50 DEG C, 30min 0.5wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 50 DEG C, 30min 0.5wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 50 DEG C, 30min 0.5wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 50 DEG C, 30min 0.5wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 50 DEG C, 30min 0.5wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 50 DEG C, 30min 0.5wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 50 DEG C, 30min 0.5wt.% cellulase in the aqueous sodium carbonate of step 1, de-alkali additives are 50 DEG C, 30min 0.5wt.% cellulase in；
In step 2, heating extraction condition is 40 DEG C, 30min；Polystyrene macroporous adsorbent resin in step 3；Ultrafiltration used in step 4
The molecular cut off of film be in 100000Da.
Human body skin moisture content is detected：
60 health volunteers are randomly selected, and forearm are cleaned with unified gentle cleaning agent before test and are bent side skin, use number
Word skin moisture detector detects each test position, is repeated 3 times respectively, draws its meansigma methods；Then by above example system
The standby moisture retention water for obtaining, 15% glycerol, normal saline are gently applied to each test position (totally 6 groups, per organize 10 people) in order, often locate
Pilot region is 2 × 2cm2, test specimen dropper drips, and after smearing, 15min, 60min and 120min are at these positions
Detect respectively again.The meansigma methodss of each detection are deducted the measured value for being the period position before not smearing.
Experimental result is referring to following table.
| 15min | 60min | 120min | |
| Embodiment 1 | 18.8 | 15.3 | 12.1 |
| Embodiment 2 | 19.2 | 15.2 | 11.9 |
| Embodiment 3 | 18.2 | 14.9 | 11.4 |
| Embodiment 4 | 19.1 | 14.7 | 11.6 |
| 15% glycerol | 8.2 | 4.6 | 3.2 |
| Normal saline | 7.5 | 3.2 | 1.0 |
As can be known from the above table, the moisture retention water that the present invention is prepared has preferably to skin moisturizing, moisturizing, is better than
Glycerol and normal saline.
Claims (6)
1. a kind of preparation method of the skin moisture-keeping water containing silkworm silk aminoacid, it is characterised in that：Comprise the following steps：
Step 1, takes silkworm silk and cleans degumming in the 10-20wt.% aqueous sodium carbonate for be placed in 5-10 times of weight, clear through deionized water
Fibroin albumen is obtained after washing；
Step 2, fibroin albumen is added in the 10-15wt.% calcium chloride solution of 2-2.5 times of weight, adjusts pH to 5-6, is added
0.2-0.6wt.% papain and 0.1-0.4wt.% trypsin, heating extraction, cooling, obtain extracting solution；
Step 3, extracting solution feeding macroporous adsorbent resin is adsorbed, is collected permeate；
Step 4, adjusts the pH to 8.0-10.0 of permeate, imports ultrafilter membrane and is filtered, obtains ultrafiltration permeate；
Step 5, in parts by weight, ultrafiltration permeate concentrating under reduced pressure, gained concentrate 0.5-1.2 part is added in glycerol 3-8 part,
Add Propylene Glycol 4-8 part, citric acid 0.4-1.2 part, water 20-40 part, Mentholum 0.2-0.4 part, butanediol 0.5-1 part, Gu
That glue 0.1-0.5 part, sodium alginate 0.2-0.5 part, Microcrystalline Cellulose 0.1-0.4 part, mixing, obtain final product.
2. the preparation method of the skin moisture-keeping water containing silkworm silk aminoacid according to claim 1, it is characterised in that：Step 1
Aqueous sodium carbonate in contain 0.2-0.5wt.% cellulase.
3. the preparation method of the skin moisture-keeping water containing silkworm silk aminoacid according to claim 1, it is characterised in that：Step 1
Middle de-alkali additives are 40-50 DEG C, 30-50min.
4. the preparation method of the skin moisture-keeping water containing silkworm silk aminoacid according to claim 1, it is characterised in that：Step 2
Middle heating extraction condition is 30-40 DEG C, 30-40min.
5. the preparation method of the skin moisture-keeping water containing silkworm silk aminoacid according to claim 1, it is characterised in that：Step 3
Middle macroporous adsorbent resin is polystyrene macroporous adsorbent resin.
6. the preparation method of the skin moisture-keeping water containing silkworm silk aminoacid according to claim 1, it is characterised in that：Step 4
Used in ultrafilter membrane molecular cut off be in 100000Da.
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| RU2674981C1 (en) * | 2017-11-29 | 2018-12-14 | Ольга Валерьевна Самодай | Method of treating acne |
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Application publication date: 20170222 |