CN106415357B - 功能集成的激光扫描显微镜 - Google Patents
功能集成的激光扫描显微镜 Download PDFInfo
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- CN106415357B CN106415357B CN201580028197.2A CN201580028197A CN106415357B CN 106415357 B CN106415357 B CN 106415357B CN 201580028197 A CN201580028197 A CN 201580028197A CN 106415357 B CN106415357 B CN 106415357B
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Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0032—Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0036—Scanning details, e.g. scanning stages
- G02B21/004—Scanning details, e.g. scanning stages fixed arrays, e.g. switchable aperture arrays
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0052—Optical details of the image generation
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0052—Optical details of the image generation
- G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/008—Details of detection or image processing, including general computer control
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/361—Optical details, e.g. image relay to the camera or image sensor
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/365—Control or image processing arrangements for digital or video microscopes
- G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Engineering & Computer Science (AREA)
- Multimedia (AREA)
- Computer Vision & Pattern Recognition (AREA)
- General Engineering & Computer Science (AREA)
- Microscoopes, Condenser (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE102014107606.0 | 2014-05-28 | ||
| DE102014107606.0A DE102014107606A1 (de) | 2014-05-28 | 2014-05-28 | Funktionsintegriertes Laser-Scanning-Mikroskop |
| PCT/EP2015/061378 WO2015181068A1 (de) | 2014-05-28 | 2015-05-22 | Funktionsintegriertes laser-scanning-mikroskop |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106415357A CN106415357A (zh) | 2017-02-15 |
| CN106415357B true CN106415357B (zh) | 2019-08-09 |
Family
ID=53276847
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201580028197.2A Active CN106415357B (zh) | 2014-05-28 | 2015-05-22 | 功能集成的激光扫描显微镜 |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US10203490B2 (enExample) |
| EP (2) | EP3798712A1 (enExample) |
| JP (1) | JP6557682B2 (enExample) |
| CN (1) | CN106415357B (enExample) |
| DE (1) | DE102014107606A1 (enExample) |
| WO (1) | WO2015181068A1 (enExample) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6768289B2 (ja) * | 2015-12-01 | 2020-10-14 | キヤノン株式会社 | 走査型顕微鏡 |
| JP6802636B2 (ja) * | 2016-03-25 | 2020-12-16 | オリンパス株式会社 | 顕微鏡システムおよび標本観察方法 |
| DE102016110433B4 (de) * | 2016-06-06 | 2022-01-27 | Carl Zeiss Microscopy Gmbh | Mikroskop und Mikroskopieverfahren |
| DE102016011227C5 (de) * | 2016-09-19 | 2020-04-09 | Leica Microsystems Cms Gmbh | Mikroskopsystem und Verfahren zur Abbildung einer Probe unter Verwendung eines Mikroskopsystems |
| US10955652B2 (en) | 2016-09-30 | 2021-03-23 | The Trustees Of Columbia University In The City Of New York | Three-dimensional imaging using swept, confocally aligned planar excitation with a Powell lens and/or deliberate misalignment |
| DE102016119730A1 (de) | 2016-10-17 | 2018-04-19 | Carl Zeiss Microscopy Gmbh | Optikgruppe für Detektionslicht für ein Mikroskop, Verfahren zur Mikroskopie und Mikroskop |
| DE102016120308A1 (de) | 2016-10-25 | 2018-04-26 | Carl Zeiss Microscopy Gmbh | Optische Anordnung, Multispot-Scanning-Mikroskop und Verfahren zum Betreiben eines Mikroskops |
| US11506877B2 (en) | 2016-11-10 | 2022-11-22 | The Trustees Of Columbia University In The City Of New York | Imaging instrument having objective axis and light sheet or light beam projector axis intersecting at less than 90 degrees |
| US10416429B2 (en) * | 2017-09-15 | 2019-09-17 | Agile Focus Designs, LLC | Dynamic focus and zoom system for use with wide-field, confocal and multiphoton microscopes |
| US10466461B2 (en) * | 2017-09-15 | 2019-11-05 | Agile Focus Designs, LLC | Dynamic focus and zoom system for use with wide-field, confocal and multiphoton microscopes |
| DE102018124129A1 (de) * | 2017-12-04 | 2019-06-06 | Leica Microsystems Cms Gmbh | Mikroskopsystem und Verfahren zur mikroskopischen Abbildung mit einem solchen Mikroskopsystem |
| WO2019204820A1 (en) * | 2018-04-20 | 2019-10-24 | Coluxa Inc. | Scanning microscope with multiplexed light sources |
| DE102018209448A1 (de) | 2018-06-13 | 2019-12-19 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Vorrichtung zur ortsaufgelösten Detektion und Verwendung einer solchen Vorrichtung |
| JP6978592B2 (ja) * | 2019-01-25 | 2021-12-08 | アジャイル フォーカス デザインズ, エルエルシーAgile Focus Designs, LLC | 広領域の共焦点及び多光子顕微鏡で用いる動的フォーカス・ズームシステム |
| CN110927947B (zh) * | 2019-11-15 | 2022-03-18 | 上海安翰医疗技术有限公司 | 相衬显微成像系统及其成像方法 |
| US11762181B2 (en) * | 2019-12-26 | 2023-09-19 | Kessler Optics and Photonics Solutions Ltd. | Scanning microscope with enhanced FOV and NA |
| EP3985423A1 (en) * | 2020-10-14 | 2022-04-20 | Leica Microsystems CMS GmbH | Control device for a confocal microscope |
| CA3151215A1 (en) * | 2021-03-19 | 2022-09-19 | Thorlabs, Inc | Compact laser scanning microscope system |
| DE102023209080A1 (de) * | 2023-09-19 | 2025-03-20 | Carl Zeiss Microscopy Gmbh | Multimodales Scanmikroskop und Verfahren zu dessen Betrieb |
| DE102023134043A1 (de) * | 2023-12-05 | 2025-06-05 | Carl Zeiss Microscopy Gmbh | Mikroskop |
| KR102888672B1 (ko) | 2025-05-14 | 2025-11-27 | 한국표준과학연구원 | 단일 광원 기반의 hts/hcs 모드 변경이 가능한 광시트 현미경 시스템 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102455500A (zh) * | 2010-10-22 | 2012-05-16 | 徕卡显微系统复合显微镜有限公司 | 具有sted片光源的spim显微镜 |
| CN103135220A (zh) * | 2011-11-28 | 2013-06-05 | 徕卡显微系统复合显微镜有限公司 | 显微镜照明系统及方法 |
| CN103743714A (zh) * | 2014-01-14 | 2014-04-23 | 苏州大猫单分子仪器研发有限公司 | 一种倾斜宽场光切片扫描成像显微系统及其成像方法 |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7028899B2 (en) * | 1999-06-07 | 2006-04-18 | Metrologic Instruments, Inc. | Method of speckle-noise pattern reduction and apparatus therefore based on reducing the temporal-coherence of the planar laser illumination beam before it illuminates the target object by applying temporal phase modulation techniques during the transmission of the plib towards the target |
| DE10344060A1 (de) | 2003-09-23 | 2005-05-04 | Zeiss Carl Jena Gmbh | Konfokales Laser-Scanning-Mikroskop |
| JP4414722B2 (ja) * | 2003-10-15 | 2010-02-10 | オリンパス株式会社 | レーザー顕微鏡 |
| DE102004034971A1 (de) * | 2004-07-16 | 2006-02-09 | Carl Zeiss Jena Gmbh | Lichtrastermikroskop mit linienförmiger Abtastung und Verwendung |
| US7567346B2 (en) * | 2006-03-01 | 2009-07-28 | General Electric Company | System and method for multimode imaging |
| JP4891057B2 (ja) * | 2006-12-27 | 2012-03-07 | オリンパス株式会社 | 共焦点レーザー走査型顕微鏡 |
| JP4924146B2 (ja) * | 2007-03-30 | 2012-04-25 | 横河電機株式会社 | 共焦点顕微鏡システム |
| DE102008054317A1 (de) * | 2008-11-03 | 2010-05-06 | Carl Zeiss Microlmaging Gmbh | Kombinationsmikroskopie |
| JP5371694B2 (ja) * | 2009-10-26 | 2013-12-18 | オリンパス株式会社 | 顕微鏡接続ユニットおよび顕微鏡システム |
| DE102009060490A1 (de) * | 2009-12-22 | 2011-06-30 | Carl Zeiss Microlmaging GmbH, 07745 | Hochauflösendes Mikroskop und Bildteileranordnung |
| EP2369401B1 (en) * | 2010-03-23 | 2015-09-23 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Optical modulator device and spatio-temporally light modulated imaging system |
| GB201019181D0 (en) * | 2010-11-12 | 2010-12-29 | Univ Leicester | Improved optical arrangement |
| DE102011077269A1 (de) * | 2011-06-09 | 2012-12-13 | Carl Zeiss Microimaging Gmbh | Hochauflösende Lumineszenzmikroskopie |
| US10222600B2 (en) * | 2015-02-23 | 2019-03-05 | The Research Foundation For The State University Of New York | Method and apparatus for tiling light sheet selective plane illumination microscopy with real-time optimized light sheet |
| US10935778B2 (en) * | 2016-11-12 | 2021-03-02 | Caliber Imaging & Diagnostics, Inc. | Confocal microscope with positionable imaging head |
-
2014
- 2014-05-28 DE DE102014107606.0A patent/DE102014107606A1/de not_active Ceased
-
2015
- 2015-05-22 CN CN201580028197.2A patent/CN106415357B/zh active Active
- 2015-05-22 WO PCT/EP2015/061378 patent/WO2015181068A1/de not_active Ceased
- 2015-05-22 JP JP2016569612A patent/JP6557682B2/ja active Active
- 2015-05-22 EP EP20201246.4A patent/EP3798712A1/de not_active Withdrawn
- 2015-05-22 US US15/314,063 patent/US10203490B2/en active Active
- 2015-05-22 EP EP15726562.0A patent/EP3149532B1/de active Active
-
2018
- 2018-11-06 US US16/182,422 patent/US10527834B2/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102455500A (zh) * | 2010-10-22 | 2012-05-16 | 徕卡显微系统复合显微镜有限公司 | 具有sted片光源的spim显微镜 |
| CN103135220A (zh) * | 2011-11-28 | 2013-06-05 | 徕卡显微系统复合显微镜有限公司 | 显微镜照明系统及方法 |
| CN103743714A (zh) * | 2014-01-14 | 2014-04-23 | 苏州大猫单分子仪器研发有限公司 | 一种倾斜宽场光切片扫描成像显微系统及其成像方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| JP6557682B2 (ja) | 2019-08-07 |
| EP3798712A1 (de) | 2021-03-31 |
| US10527834B2 (en) | 2020-01-07 |
| US20170199362A1 (en) | 2017-07-13 |
| DE102014107606A1 (de) | 2015-12-03 |
| EP3149532B1 (de) | 2020-10-28 |
| WO2015181068A1 (de) | 2015-12-03 |
| CN106415357A (zh) | 2017-02-15 |
| JP2017517767A (ja) | 2017-06-29 |
| US10203490B2 (en) | 2019-02-12 |
| US20190227290A1 (en) | 2019-07-25 |
| EP3149532A1 (de) | 2017-04-05 |
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