CN106399171B - A kind of atrophy bacillus and its biological deodorizing microbial inoculum for deodorization - Google Patents
A kind of atrophy bacillus and its biological deodorizing microbial inoculum for deodorization Download PDFInfo
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- CN106399171B CN106399171B CN201610829244.XA CN201610829244A CN106399171B CN 106399171 B CN106399171 B CN 106399171B CN 201610829244 A CN201610829244 A CN 201610829244A CN 106399171 B CN106399171 B CN 106399171B
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Abstract
The invention discloses a kind of atrophy bacillus (Bacillus atrophaeus), are preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number CGMCC No.12466.The invention also discloses more than one to state biological deodorizing microbial inoculum that atrophy bacillus is active constituent and preparation method thereof, application method；The invention also discloses the application of above-mentioned atrophy bacillus and biological deodorizing microbial inoculum in biological deodorizing.Atrophy Bacillus strain of the invention can well be grown in rubbish, waste, advantageous ecological niche is occupied, while absorbing decomposition foul gas, also inhibits and produces stench microbial growth, foul gas is reduced from source to generate, and will not be caused damages to ambient enviroment.Bacterial preparation process of the invention is simple, and preparation time is short, low in cost, and deodorizing effect is efficient, and mode is simple, is suitable for popularization and application.
Description
Technical field
The present invention relates to Applied Biotechnology fields, more particularly to a kind of atrophy bacillus for deodorization
(Bacillus atrophaeus), the biological deodorizing microbial inoculum containing it, preparation method, application method and in biological deodorizing
Application.
Background technique
Stench refers to that all stimulation olfactory organs cause people unhappy and the gas general name of damage human habitat.So far
The present just has more than 4000 kinds with regard to sentient odorant with the smell of people, has tens wherein biggish to human health damage
Kind.Life source and industrial source can be divided by stench generating source.Life source refers to the stench generated in daily life, as household is washed
Between hand, public lavatory, town sewer, refuse transfer station and contaminated lake, ditch etc.；Industrial source refers to industrial production
The stench of middle generation, such as cultivation factory, chemical plant, pharmaceutical factory, food processing factory, coating factory.The smelly mood for not only influencing people, if
In high concentration stench environment or it is chronically at the stench environment of low concentration, the body of people will receive grievous injury.Long-term sucking
Irritating foul smell can damage respiratory mucosa, interfere normal respiratory function；Often in contact with odorant, the sense that engenders a repugnance,
The symptoms such as Nausea and vomiting；Long-term sucking stench can cause body anoxic, serious cardiovascular disease etc.；Stench can make to secrete function
Energy disorder, and influence the metabolic activity of body；Make smell depigmentation, it is serious to generate toxic action to nervous system.For a long time by
The continuous action of stench can make one irritated, melancholy, insomnia, absent minded, hypomnesia, to make study and work efficiency
It reduces.Meanwhile foul gas can also influence the growth and yield of animals and plants.
Research in relation to deodorization technology has become an important link in environmental improvement engineering.Due to odor polluting source
Distribution is wide, concentration is low, complicated component, is not easy the characteristics of detecting the removing of stench is made to be different from the improvement that general atmosphere pollutes.
Currently, the administering method for stench mainly has: physical deodorization method, chemical deodorization method, biological odor removal method.Biological deodorizing is exactly benefit
Odorant absorption, absorption, decomposition are converted into odorless substance with microorganism, reach deodorization purpose.The principle of biological deodorizing is
Odorant is degraded using the metabolic activity of microorganism, generally can be divided into three processes: 1. stench is liquid by gas phase transition
The course of dissolution of phase；2. cell wall and cell membrane of the odor pollutant in liquid phase through microorganism are adsorbed, absorb, undissolved smelly
Gas substance is first attached to outside microbial body, and the ectoenzyme of microorganism secretions is waited to be broken down into penetrating into again after solable matter carefully
Born of the same parents；3. the odorous substance for entering microbial cell is decomposed for Institute of Micro-biology as nutriment, utilized, foul smell is enable to remove.
Summary of the invention
The invention solves first technical problem be to provide a kind of atrophy bacillus for being efficiently used for deodorization.
The invention solves second technical problem be to provide a kind of biological deodorizing microbial inoculum.
The invention solves third technical problem be to provide the preparation method of biological deodorizing microbial inoculum a kind of.
The invention solves the 4th technical problem be to provide a kind of atrophy bacillus, biological deodorizing microbial inoculum in biology
Application in deodorization.
The invention solves the 5th technical problem be to provide the application method of biological deodorizing microbial inoculum a kind of.
In order to solve the above technical problems, the present invention adopts the following technical scheme:
A kind of atrophy bacillus (Bacillus atrophaeus), the related preservation information being directed to are as follows:
Depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center；Address: Chaoyang District, Beijing City
The institute of North Star West Road 1, Institute of Microorganism, Academia Sinica；Preservation date: on May 19th, 2016；Deposit number: CGMCC
No.12466；Systematic name: atrophy bacillus (Bacillus atrophaeus).
A kind of biological deodorizing microbial inoculum, active constituent are the atrophy gemma that above-mentioned deposit number is CGMCC No.12466
Bacillus.
Above-mentioned biological deodorizing microbial inoculum, preferably liquid bacterial agent.
Above-mentioned biological deodorizing microbial inoculum, the viable count are more than or equal to 1010CFU/mL。
The preparation method of above-mentioned biological deodorizing microbial inoculum, includes the following steps:
(1) first the atrophy bacillus that deposit number is CGMCC No.12466 is activated in LB solid slope culture medium；
(2) inoculate the culture of microbial inoculum fluid nutrient medium 24 hours or more, adjust bacterial strain bacteria containing amount be more than or equal to
1010Liquid bio deodorizing microorganism is prepared in CFU/mL.
LB solid slope culture medium in above-mentioned steps (1) is used for actication of culture, formula are as follows: tryptone 10g,
Yeast extract 5g, NaCl 10g, water 1000mL, agar 15-20g, pH6.5-7.0.
The formula of microbial inoculum fluid nutrient medium in above-mentioned steps (2) are as follows: brown sugar 20g, tryptone 10g, powdered beef 5g,
NaCl 10g, water 1000mL, pH6.5-7.0.
Above-mentioned atrophy bacillus or biological deodorizing microbial inoculum can be used for carrying out biological deodorizing.Such as, it is applied at rubbish, livestock and poultry
The deodorization of at waste or toilet.
The application method of above-mentioned biological deodorizing microbial inoculum are as follows: biological deodorizing microbial inoculum is diluted with water by 1:200, is sprayed on to be processed
Place.
By using above-mentioned technical proposal, the present invention has at least the following advantages:
1, bacterial strain is screened and is obtained from being coerced for a long time in selection environment by refuse pollution, can well be grown in rubbish, waste,
Occupy advantageous ecological niche；The foul gas such as sulfide, nitrogen oxides, halide and hydro carbons can be absorbed and be decomposed, eliminate stench.?
It while absorbing decomposition foul gas, also inhibits and produces stench microbial growth, foul gas is reduced from source and is generated,
And it will not cause damages to ambient enviroment.
2, microbial inoculum deodorizing effect is efficient, and preparation method is simple, and preparation time is short, low in cost.
3, microbial inoculum is easy to use, and it is convenient to produce, and easily puts goods on the market, forms commercialization.
Specific embodiment
Explain the present invention in detail with reference to embodiments.Embodiment to facilitate the understanding of the present invention, but not
Limitation of the present invention.
Embodiment 1
A kind of biological deodorizing microbial inoculum, is prepared as follows:
(1) the access LB solid slope culture medium activation of atrophy bacillus (Bacillus atrophaeus) strain is standby
With.LB solid medium, formula are as follows: tryptone 10g, yeast extract 5g, NaCl 10g, water 1000mL, agar 15-
20g、pH 6.5-7.0。
(2) the microbial inoculum fluid nutrient medium for crossing sterilization treatment quantifies spare, the formula of microbial inoculum fluid nutrient medium are as follows: brown sugar
20g, tryptone 10g, powdered beef 5g, NaCl 10g, water 1000mL, pH6.5-7.0.
(3) strain after activation is respectively connected to expand in the fluid nutrient medium that sterilization treatment is crossed culture 24 hours or more.
(4) by cultured each bacterium solution counting adjusting, (it is 10 that the present embodiment, which preferably adjusts the bacteria containing amount of bacterial strain,10CFU/
ML), as liquid bio deodorizing microorganism.Liquid bacterial agent is quantitatively bottled, and is kept in dark place at low temperature.
The main indicator of the liquid bio deodorizing microorganism of above-mentioned preparation are as follows: liquid complex microorganism preparations: viable count is greater than
Equal to 10,000,000,000 CFU/mL；pH 5-7；Suspension, color are dark red.
The biological deodorizing microbial inoculum can be used at rubbish (such as waste treatment facility), release with toilet stench at livestock and poultry feces
Put source.Its application method are as follows: liquid bio deodorizing microorganism is diluted with distilled water by 1:200, is sprayed on waste treatment facility, livestock and poultry
At waste and toilet.
Effect example
The 50ml landfill leachate for picking up from Nangong rubbish bacterial manure factory is put into 25L plastic barrel, closed standing is after 30 minutes
Start to acquire background odor concentration, ammonia, one group of hydrogen sulfide gas sample, ammonia, hydrogen sulfide respectively acquire 10 minutes, and odor concentration is wink
When sample.Then it takes 10ml biological deodorizing microbial inoculum to be diluted to 1L, every 5 minutes in plastic barrel is sprayed, until deodorizing microorganism
Dilution exhausts.After the completion of spraying, start to acquire odor concentration, ammonia, one group of hydrogen sulfide gas sample after standing 30 minutes,
Sampling time is the same as consistent before deodorization.It acquires sample and deodorizing microorganism sprinkling keeps plastic barrel air-tight state in the process.
Experimental result is shown in Table 1.Wherein ammonia, concentration of hydrogen sulfide unit are mg/m3, odor concentration unit dimensionless.
1 deodorizing microorganism of table index contrast table before and after the processing
| Index | Before deodorization | After deodorization |
| Ammonia concentration | 1.19 | 0.201 |
| Odor concentration | 525 | 51 |
| Concentration of hydrogen sulfide | 0.027 | Less than 0.005 |
As seen from the above table, biological deodorizing microbial inoculum, odor strength, ammonia concentration, concentration of hydrogen sulfide are added in rubbish soak
It is substantially reduced than control, wherein ammonia concentration, which reduces by 83.1%, odor strength, reduces by 90.3%, and concentration of hydrogen sulfide reduces
81.5% or more.
The above described is only a preferred embodiment of the present invention, be not intended to limit the present invention in any form, this
Field technical staff makes a little simple modification, equivalent variations or modification using the technology contents of the disclosure above, all falls within this hair
In bright protection scope.
Claims (10)
1. a kind of atrophy bacillus (Bacillus atrophaeus), which is characterized in that be preserved in Chinese microorganism strain guarantor
Administration committee's common micro-organisms center is hidden, deposit number is CGMCC No.12466.
2. a kind of biological deodorizing microbial inoculum, which is characterized in that its active constituent is that deposit number described in claim 1 is CGMCC
The atrophy bacillus of No.12466.
3. biological deodorizing microbial inoculum according to claim 2, which is characterized in that be liquid bacterial agent.
4. biological deodorizing microbial inoculum according to claim 3, which is characterized in that the viable count of the liquid bacterial agent is more than or equal to
1010CFU/mL。
5. according to the preparation method of the described in any item biological deodorizing microbial inoculums of claim 2-4, which is characterized in that including walking as follows
It is rapid:
(1) first the atrophy bacillus that deposit number is CGMCC No.12466 is activated in LB solid slope culture medium；
(2) it inoculates the culture of microbial inoculum fluid nutrient medium 24 hours or more, the bacteria containing amount for adjusting bacterial strain is more than or equal to 1010CFU/
Liquid bio deodorizing microorganism is prepared in mL.
6. the preparation method of biological deodorizing microbial inoculum according to claim 5, which is characterized in that the LB in the step (1)
Solid slope culture medium, formula are as follows: tryptone 10g, yeast extract 5g, NaCl 10g, water 1000mL, agar 15-
20g、pH 6.5-7.0。
7. the preparation method of biological deodorizing microbial inoculum according to claim 5, which is characterized in that in the step (2)
The formula of microbial inoculum fluid nutrient medium are as follows: brown sugar 20g, tryptone 10g, powdered beef 5g, NaCl 10g, water 1000mL,
pH6.5-7.0。
8. described in atrophy bacillus (Bacillus atrophaeus) described in claim 1 or claim any one of 2-4
Application of the biological deodorizing microbial inoculum in biological deodorizing.
9. application according to claim 8, which is characterized in that applied at rubbish, at livestock and poultry feces or toilet removes
It is smelly.
10. the application method of biological deodorizing microbial inoculum according to claim 4, which is characterized in that biological deodorizing microbial inoculum water
It is diluted by 1:200, is sprayed on place to be processed.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103228264A (en) * | 2010-06-24 | 2013-07-31 | 诺维信生物股份有限公司 | Methods and compositions for reducing body odor |
| CN103476921A (en) * | 2011-02-15 | 2013-12-25 | 诺维信生物股份有限公司 | Mitigation of odor in cleaning machines and cleaning processes |
| CN105051177A (en) * | 2012-12-31 | 2015-11-11 | 瑞纳戈若有限公司 | New bacteria and consortia for the reduction of amonia and/or methane emission in manure or soil |
| CN105483045A (en) * | 2015-12-23 | 2016-04-13 | 青岛泰西科环保工程有限公司 | Strain composition for treating VOCs containing waste gas and method for treating VOCs containing waste gas |
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- 2016-09-18 CN CN201610829244.XA patent/CN106399171B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103228264A (en) * | 2010-06-24 | 2013-07-31 | 诺维信生物股份有限公司 | Methods and compositions for reducing body odor |
| CN103476921A (en) * | 2011-02-15 | 2013-12-25 | 诺维信生物股份有限公司 | Mitigation of odor in cleaning machines and cleaning processes |
| CN105051177A (en) * | 2012-12-31 | 2015-11-11 | 瑞纳戈若有限公司 | New bacteria and consortia for the reduction of amonia and/or methane emission in manure or soil |
| CN105483045A (en) * | 2015-12-23 | 2016-04-13 | 青岛泰西科环保工程有限公司 | Strain composition for treating VOCs containing waste gas and method for treating VOCs containing waste gas |
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Inventor after: Wang Xin Inventor after: Wang Jinan Inventor after: Zhu Jiagen Inventor after: He Wei Inventor after: Wang Yan Inventor after: Liu Binjie Inventor after: Wang Cuixia Inventor before: Wang Xin |
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