CN106386591A - Method for establishing erucic acid induced fish enteritis model - Google Patents
Method for establishing erucic acid induced fish enteritis model Download PDFInfo
- Publication number
- CN106386591A CN106386591A CN201610276359.0A CN201610276359A CN106386591A CN 106386591 A CN106386591 A CN 106386591A CN 201610276359 A CN201610276359 A CN 201610276359A CN 106386591 A CN106386591 A CN 106386591A
- Authority
- CN
- China
- Prior art keywords
- fish
- erucic acid
- colitis model
- feed
- model
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000251468 Actinopterygii Species 0.000 title claims abstract description 91
- DPUOLQHDNGRHBS-UHFFFAOYSA-N Brassidinsaeure Natural products CCCCCCCCC=CCCCCCCCCCCCC(O)=O DPUOLQHDNGRHBS-UHFFFAOYSA-N 0.000 title claims abstract description 53
- URXZXNYJPAJJOQ-UHFFFAOYSA-N Erucic acid Natural products CCCCCCC=CCCCCCCCCCCCC(O)=O URXZXNYJPAJJOQ-UHFFFAOYSA-N 0.000 title claims abstract description 53
- DPUOLQHDNGRHBS-KTKRTIGZSA-N erucic acid Chemical compound CCCCCCCC\C=C/CCCCCCCCCCCC(O)=O DPUOLQHDNGRHBS-KTKRTIGZSA-N 0.000 title claims abstract description 53
- 238000000034 method Methods 0.000 title claims abstract description 20
- 208000004232 Enteritis Diseases 0.000 title abstract description 21
- 206010009887 colitis Diseases 0.000 claims description 34
- 238000002474 experimental method Methods 0.000 claims description 23
- 230000006698 induction Effects 0.000 claims description 8
- 241000252230 Ctenopharyngodon idella Species 0.000 claims description 6
- 108090000623 proteins and genes Proteins 0.000 claims description 6
- 102000004169 proteins and genes Human genes 0.000 claims description 6
- 230000037396 body weight Effects 0.000 claims description 5
- 229920002261 Corn starch Polymers 0.000 claims description 3
- 235000019733 Fish meal Nutrition 0.000 claims description 3
- 240000007594 Oryza sativa Species 0.000 claims description 3
- 235000007164 Oryza sativa Nutrition 0.000 claims description 3
- 229920002472 Starch Polymers 0.000 claims description 3
- 239000008120 corn starch Substances 0.000 claims description 3
- 229940099112 cornstarch Drugs 0.000 claims description 3
- 239000004467 fishmeal Substances 0.000 claims description 3
- 239000001963 growth medium Substances 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 235000009566 rice Nutrition 0.000 claims description 3
- 235000019698 starch Nutrition 0.000 claims description 3
- 239000008107 starch Substances 0.000 claims description 3
- 238000005453 pelletization Methods 0.000 claims description 2
- 210000001035 gastrointestinal tract Anatomy 0.000 abstract description 13
- 238000004519 manufacturing process Methods 0.000 abstract description 11
- 230000036039 immunity Effects 0.000 abstract description 4
- 238000011161 development Methods 0.000 abstract description 3
- 210000003205 muscle Anatomy 0.000 abstract description 2
- 230000001717 pathogenic effect Effects 0.000 abstract 1
- 210000000981 epithelium Anatomy 0.000 description 16
- 235000013372 meat Nutrition 0.000 description 9
- 240000002791 Brassica napus Species 0.000 description 7
- 235000004977 Brassica sinapistrum Nutrition 0.000 description 7
- 238000007689 inspection Methods 0.000 description 6
- 201000010099 disease Diseases 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 210000001842 enterocyte Anatomy 0.000 description 5
- 210000000952 spleen Anatomy 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 235000019484 Rapeseed oil Nutrition 0.000 description 3
- 230000003247 decreasing effect Effects 0.000 description 3
- 230000007119 pathological manifestation Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 2
- 108010022579 ATP dependent 26S protease Proteins 0.000 description 2
- 241001270131 Agaricus moelleri Species 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- 206010053759 Growth retardation Diseases 0.000 description 2
- 206010020565 Hyperaemia Diseases 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 244000046052 Phaseolus vulgaris Species 0.000 description 2
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- 238000009360 aquaculture Methods 0.000 description 2
- 244000144974 aquaculture Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000004626 essential fatty acids Nutrition 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- 231100000001 growth retardation Toxicity 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 210000000936 intestine Anatomy 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 235000010755 mineral Nutrition 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 230000002477 vacuolizing effect Effects 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 150000003722 vitamin derivatives Chemical class 0.000 description 2
- 241000607528 Aeromonas hydrophila Species 0.000 description 1
- 235000019750 Crude protein Nutrition 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 229930003427 Vitamin E Natural products 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 235000019621 digestibility Nutrition 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 208000010824 fish disease Diseases 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000029052 metamorphosis Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 230000003950 pathogenic mechanism Effects 0.000 description 1
- 239000010773 plant oil Substances 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000010499 rapseed oil Substances 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 239000011669 selenium Substances 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 229940046009 vitamin E Drugs 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2207/00—Modified animals
- A01K2207/20—Animals treated with compounds which are neither proteins nor nucleic acids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/40—Fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/03—Animal model, e.g. for test or diseases
- A01K2267/035—Animal model for multifactorial diseases
- A01K2267/0368—Animal model for inflammation
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Immunology (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Marine Sciences & Fisheries (AREA)
- Animal Behavior & Ethology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a method for establishing an erucic acid induced fish enteritis model. The method comprises the following steps: selecting normal cultured fishes as objects for establishing an enteritis model; and feeding the cultured fishes with an experimental feed in which the content of erucic acid is 12g/kg, thereby obtaining the erucic acid induced fish enteritis model. The method for establishing the erucic acid induced fish enteritis model, disclosed by the invention, has smallest influence on production performance, growth development of intestinal tracts, immunity and muscle quality of the fishes, but caused enteritis pathogenic manifestations are most suitable, and the model is stable and reliable, and has good reproducibility.
Description
Technical field
The present invention relates to the method for building up of animal model is and in particular to a kind of erucic acid induces the method for building up of fish colitis model.
Background technology
With the development of aquaculture and feed industry, the shortage of feed protein resource has become world's common problem, becomes one of serious restrictive factor of culture fishery development.Rapeseed is one of main oil crops of China, and its yield accounts for more than the 1/3 of plant oil yield, occupies first place in the world.With the continuous improvement of rapeseed planting technology, production grows a lot, and per mu yield improves constantly, and its planting range constantly expands.In addition to the Yangtze river basin and southwest, northwest etc. are main producing region, because its available Winter idle land plantation, do not strive ground with field crop, rapeseed cultivation is also being greatly developed it is contemplated that the yield of China's rapeseed can improve constantly in other areas.
Rapeseed dregs is the accessory substance after rapeseed oil, its crude protein content should be more than 32%, crude fiber content is below 12%, containing abundant lysine and sulfur-containing amino acid, macro- and trace-element, wherein calcium, selenium, iron, magnesium, manganese, zinc content higher than dregs of beans, phosphorus content is 2 times of dregs of beans, yield is again high simultaneously, cheap, convenient sources, these features make rapeseed dregs become the major protein source of China's main cultured fishes feed, can effectively alleviate the problem of the shortage of China's aquaculture feed protein resource.Rapeseed oil essential fatty acid content is high, rich in vitamin and mineral matters such as vitamin E, calcium and phosphorus simultaneously, fish may be up to 99% to rape oil digestibility, the nutriment needs such as essential fatty acid, vitamin and mineral matter of fish can be met, be the main source of fat in fish feed.And due to containing the ANFs such as higher erucic acid in rapeseed dregs and rapeseed oil, harm can be produced to healthy fish and growth.But there is which kind of harm to fish with regard to erucic acid, the research of its toxic action mechanism is considerably less actually.
Enteritis is cultured fishes, such as grass carp, one of most common disease.The fatal rate of fish enteritis, generally 50%, sometimes even up to 90%, is harm cultured freshwater fish disease the most serious.This disease causes cultured fishes to be up to the catastrophic loss of several hundred million RMB every year.
According to the physilogical characteristics of cultured fishes, set up stable fish colitis model, be the research of fish enteritis pathomechanism and the medicine/of new preventing and treating/or alleviation fish enteritis or the good tool of nutrition prescription screening.And how to set up that to stablize suitable fish colitis model be current urgent problem.Under many circumstances, according to the fish colitis model being not suitable for method foundation, it is easily caused fish enteritis and becomes excessively serious and then lead to fish dead, the death rate is too high, and fish colitis model is unstable, so that fish colitis model is applied and is restricted.
Content of the invention
It is an object of the invention to provide a kind of erucic acid induces the method for building up of fish colitis model so that the fish colitis model set up is stable, the enteritis pathological manifestations causing are optimal, both will not be excessively slight and will not be excessively serious.
The present invention is achieved through the following technical solutions：
A kind of erucic acid induces the method for building up of fish colitis model, comprises the following steps：
Select normal cultivation fish as the object setting up colitis model；
Take the experiment feed that content of erucic acid is 12g/kg, feed above-mentioned cultivation fish, obtain the fish colitis model of erucic acid induction.
Present invention only requires feeding can obtain the suitable fish colitis model of fish enteritis reason performance for 60 days, and the production performance of fish colitis model, immunocompetence and the meat quality that obtain are declined slightly, without the serious growth hindering fish.
The present invention is found by research：When content of erucic acid is less than 12g/kg, the production performance of fish, enteron aisle grow, the fall of immunocompetence and meat quality is less, but fish enteritis reason performance is also excessively slight；When content of erucic acid is higher than 12g/kg, the production performance of fish, enteron aisle grow, immunocompetence and meat quality have significant decline, and fish enteritis reason performance is excessively serious, very serious vacuolation in gut epithelium, the enterocyte on pleat top comes off very seriously, and the 26S Proteasome Structure and Function of enteric epithelium is badly damaged, leads to fish growth retardation, the incidence of disease significantly raises, or even occurs fish dead；When content of erucic acid is for 12g/kg, cause gut epithelium that obvious cavity occurs, the enterocyte of pleat top and both sides comes off substantially, and top is changed into flat-top, fish enteritis reason performance be optimal, and its production performance, enteron aisle grow, immunocompetence and meat quality fall less.
A kind of erucic acid induces the method for building up of fish colitis model, comprises the following steps：
Select the growth medium cultivation fish that body weight is 230-730g as the object setting up colitis model；
Erucic acid is mixed in main component be fish meal, FSPC, in the feed of rice protein powder, cornstarch and alphalise starch, be configured to the experiment feed that content of erucic acid is 12g/kg；
The amount that above-mentioned experiment feed is pressed 3wt% fish body weight daily feeds 60 days, obtains the fish colitis model of erucic acid induction.
Wherein, prepared experiment feed, after pelletizing, is placed in 4 DEG C of temperature and saves backup.
Wherein, described cultivation fish includes but is not limited to grass carp.
The present invention compared with prior art, has such advantages as and beneficial effect：The present invention is grown on the production performance of fish, enteron aisle using the method for building up that erucic acid induces fish colitis model, immunocompetence and meat quality impact are minimum, but the enteritis pathological manifestations causing are optimal, and model stability is reliable, favorable reproducibility.
Brief description
Accompanying drawing described herein is used for providing the embodiment of the present invention is further understood, and constitutes the part of the application, does not constitute the restriction to the embodiment of the present invention.In the accompanying drawings：
Fig. 1 is the microphoto of the experiment fish enteron aisle application histotomy inspection gut epithelium form of the embodiment of the present invention 1, and in the experiment feed of feeding, content of erucic acid is 0 g/kg；
Fig. 2 is the microphoto of the experiment fish enteron aisle application histotomy inspection gut epithelium form of the embodiment of the present invention 2, and in the experiment feed of feeding, content of erucic acid is 4.0g/kg；
Fig. 3 is the microphoto of the experiment fish enteron aisle application histotomy inspection gut epithelium form of the embodiment of the present invention 3, and in the experiment feed of feeding, content of erucic acid is 8.0g/kg；
Fig. 4 is the microphoto of the experiment fish enteron aisle application histotomy inspection gut epithelium form of the embodiment of the present invention 4, and in the experiment feed of feeding, content of erucic acid is 12.0g/kg；
Fig. 5 is the microphoto of the experiment fish enteron aisle application histotomy inspection gut epithelium form of the embodiment of the present invention 5, and in the experiment feed of feeding, content of erucic acid is 16.0g/kg；
Fig. 6 is the microphoto of the experiment fish enteron aisle application histotomy inspection gut epithelium form of the embodiment of the present invention 6, and in the experiment feed of feeding, content of erucic acid is 66.0g/kg.
Specific embodiment
For making the object, technical solutions and advantages of the present invention become more apparent, with reference to embodiment and accompanying drawing, the present invention is described in further detail, and the exemplary embodiment of the present invention and its explanation are only used for explaining the present invention, not as a limitation of the invention.
Embodiment l-6：A kind of method for building up of the fish colitis model of erucic acid induction, comprises the following steps：
1st, according to a conventional method using growth medium grass carp 540 tail of 230-730g as the object setting up colitis model, be randomly divided into 6 treatment groups, every group 3 is repeated, each repeat 30 tail fishes.
2nd, the experiment feed containing erucic acid for the preparation
Erucic acid is uniformly mixed in main component be fish meal, FSPC, in the feed of rice protein powder, cornstarch and alphalise starch, be configured to respectively content of erucic acid be 0,4.0,8.0,12.0,16.0 and 66.0 g/kg experiment feed, pelletize, be placed in 4 DEG C of refrigerators and save backup；
3rd, the foundation of model
The grass carp that step 1 is selected is raised in 1 cubic metre of net cage, feeds the experiment feed 60 days prepared by above-mentioned steps 2 daily as the amount of 3wt% fish body weight respectively, obtains the fish colitis model of erucic acid induction.
The fish colitis model product test that above-described embodiment l-6 obtains is as follows：
The erucic acid induction fish colitis model of 4.1 pairs of embodiment l-6 acquisitions measures production performance, enteron aisle grows and meat quality, and result is recorded in table 1 below.
Table 1
Note：In upper table 1, FE is feed efficiency, can reflect the utilization ratio of feed；PWG is percentage weight increase, can reflect the speed of growth of fish；ISI and ILI is respectively intestines body ratio and index of the intestines length, can reflect growing of enteron aisle；Shearing force and pH can reflect the quality of muscle；Subscript a, b, c, d, e are to characterize the significance of difference between each group of data using the labelled notation of Multiple range test, the labelled notation of this Multiple range test be those skilled in the art commonly use mark the method whether between each data with significant difference.
As can be seen that after content of erucic acid is more than 12g/kg, the production performance of fish is remarkably decreased, and enteron aisle grows and is seriously obstructed, and meat quality is decreased obviously from upper table 1.When content of erucic acid is less than or equal to 12g/kg, the production performance of fish, enteron aisle grow and meat quality is all declined slightly, but impact is less.
The erucic acid induction fish colitis model of 4.2 pairs of embodiment l-6 acquisitions carries out attacking poison by the way of lumbar injection Aeromonas hydrophila, to detect fish enteron aisle, spleen and head-kidney immunity and germ resistivity, shown in its result table specific as follows 2.
Table 2
Note：In upper table 2, subscript a, b, c, d, e are the significances of difference to be characterized using the labelled notation of Multiple range test between each group of data.
From upper table 2 as can be seen that after content of erucic acid is more than 12g/kg, the enteron aisle of fish, spleen and head-kidney immunity and germ resistivity are remarkably decreased, and enteron aisle red and swollen hyperemia incidence significantly raises, and spleen and the head-kidney hyperemia enlargement incidence of disease also dramatically increase.And when content of erucic acid is less than or equal to 12g/kg, the enteron aisle of fish, spleen and head-kidney immunity and germ resistivity are declined slightly.
The erucic acid of 4.3 pairs of embodiment l-6 acquisitions induces fish colitis model enteron aisle application histotomy to check the metamorphosis of gut epithelium, and its result is as shown in figures 1 to 6.It can be seen that, as shown in figure 1, when content of erucic acid is 0 g/kg, enteric epithelium structural integrity, form is normal；As Figure 2-3, when content of erucic acid is more than 0 g/kg and is less than 12g/kg, the slight cavity of gut epithelium, shown in Fig. 2, coming off occurs in rarely seen pleat apical epithelium, two epithelial structure are more complete, and the enterocyte of pleat top shown in Fig. 3 and both sides all comes off, but pleat remains in that normal shape, therefore, the performance of fish enteritis reason is excessively slight；As shown in figure 4, when content of erucic acid is for 12g/kg, gut epithelium obvious cavity and increases, and the enterocyte of pleat top and both sides comes off substantially, top is changed into flat-top, and fish enteritis reason performance is optimal；As seen in figs. 5-6, after content of erucic acid is more than 12g/kg, very serious vacuolation in gut epithelium, the enterocyte on pleat top comes off very serious, the 26S Proteasome Structure and Function of enteric epithelium is badly damaged, and fish enteritis reason performance is excessively serious, leads to fish growth retardation, the incidence of disease significantly raises, or even occurs fish dead.
To sum up, the present invention is grown on the production performance of fish, enteron aisle using the method for building up that the erucic acid of 12g/kg induces fish colitis model, immunocompetence and meat quality impact are minimum, but the enteritis pathological manifestations causing are optimal, model stability is reliable, favorable reproducibility.And method of the present invention can be widely used in various cultivation fishes, the including but not limited to grass carp cited by embodiment.
Above-described specific embodiment; the purpose of the present invention, technical scheme and beneficial effect are further described; be should be understood that; the foregoing is only the specific embodiment of the present invention; the protection domain being not intended to limit the present invention; all any modification, equivalent substitution and improvement within the spirit and principles in the present invention, done etc., should be included within the scope of the present invention.
Claims (4)
1. a kind of erucic acid induces the method for building up of fish colitis model it is characterised in that comprising the following steps：
Select normal cultivation fish as the object setting up colitis model；
Take the experiment feed that content of erucic acid is 12g/kg, feed above-mentioned cultivation fish, obtain the fish colitis model of erucic acid induction.
2. a kind of erucic acid induces the method for building up of fish colitis model it is characterised in that comprising the following steps：
Select the growth medium cultivation fish that body weight is 230-730g as the object setting up colitis model；
Erucic acid is mixed in main component be fish meal, FSPC, in the feed of rice protein powder, cornstarch and alphalise starch, be configured to the experiment feed that content of erucic acid is 12g/kg；
The amount that above-mentioned experiment feed is pressed 3wt% fish body weight daily feeds 60 days, obtains the fish colitis model of erucic acid induction.
3. erucic acid according to claim 2 induces the method for building up of fish colitis model it is characterised in that prepared experiment feed, after pelletizing, is placed in 4 DEG C of temperature and saves backup.
4. erucic acid according to claim 2 induces the method for building up of fish colitis model it is characterised in that described cultivation fish includes but is not limited to grass carp.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610276359.0A CN106386591B (en) | 2016-04-29 | 2016-04-29 | A kind of method for building up of erucic acid induction fish colitis model |
| FR1753667A FR3050615B1 (en) | 2016-04-29 | 2017-04-27 | METHOD FOR ESTABLISHING A MODEL OF FISH ENTERITIS INDUCED BY ERUCIC ACID. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610276359.0A CN106386591B (en) | 2016-04-29 | 2016-04-29 | A kind of method for building up of erucic acid induction fish colitis model |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106386591A true CN106386591A (en) | 2017-02-15 |
| CN106386591B CN106386591B (en) | 2019-03-19 |
Family
ID=58006222
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610276359.0A Active CN106386591B (en) | 2016-04-29 | 2016-04-29 | A kind of method for building up of erucic acid induction fish colitis model |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN106386591B (en) |
| FR (1) | FR3050615B1 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109247270A (en) * | 2018-08-07 | 2019-01-22 | 广东省实验动物监测所 | A kind of method of quick screening small test fish building pancreas fatty infiltration model |
| CN110447787A (en) * | 2018-05-07 | 2019-11-15 | 中国科学院水生生物研究所 | Galanthamine is preventing and treating the application in fish enteritis caused by bean-dregs feed |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113016714B (en) * | 2021-03-04 | 2023-08-22 | 湖南中医药大学 | Preparation method of animal model with diarrhea and food stagnation gastrointestinal symptoms |
| CN116369249B (en) * | 2023-01-13 | 2024-05-17 | 四川轻化工大学 | Construction method of zebra fish enteritis model |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102232963A (en) * | 2010-04-22 | 2011-11-09 | 郑萍 | Method for establishing ulcerative colitis chemically cancer-induced animal model |
| CN102813671A (en) * | 2012-09-05 | 2012-12-12 | 辉源生物科技（上海）有限公司 | Method for building dextran sodium sulphate (DSS) induced enteritis mouse model |
| CN103301162A (en) * | 2013-04-17 | 2013-09-18 | 苏州大学 | Method for establishing fish inflammatory bowel disease model and established model thereof |
| CN104046592A (en) * | 2014-05-16 | 2014-09-17 | 浙江大学 | Comprehensive ulcerative colitis model using C-BF to protect epithelial cell lines of mouse and human being with colorectal carcinoma |
| EP2880989A1 (en) * | 2012-08-03 | 2015-06-10 | Kyowa Hakko Bio Co., Ltd. | Foodstuff for cultured fish and method for promoting growth of cultured fish using same |
-
2016
- 2016-04-29 CN CN201610276359.0A patent/CN106386591B/en active Active
-
2017
- 2017-04-27 FR FR1753667A patent/FR3050615B1/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102232963A (en) * | 2010-04-22 | 2011-11-09 | 郑萍 | Method for establishing ulcerative colitis chemically cancer-induced animal model |
| EP2880989A1 (en) * | 2012-08-03 | 2015-06-10 | Kyowa Hakko Bio Co., Ltd. | Foodstuff for cultured fish and method for promoting growth of cultured fish using same |
| CN102813671A (en) * | 2012-09-05 | 2012-12-12 | 辉源生物科技（上海）有限公司 | Method for building dextran sodium sulphate (DSS) induced enteritis mouse model |
| CN103301162A (en) * | 2013-04-17 | 2013-09-18 | 苏州大学 | Method for establishing fish inflammatory bowel disease model and established model thereof |
| CN104046592A (en) * | 2014-05-16 | 2014-09-17 | 浙江大学 | Comprehensive ulcerative colitis model using C-BF to protect epithelial cell lines of mouse and human being with colorectal carcinoma |
Non-Patent Citations (1)
| Title |
|---|
| 宋霖: "黄颡鱼和中华绒螯蟹胃肠道形态和功能对投饲的响应", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110447787A (en) * | 2018-05-07 | 2019-11-15 | 中国科学院水生生物研究所 | Galanthamine is preventing and treating the application in fish enteritis caused by bean-dregs feed |
| CN110447787B (en) * | 2018-05-07 | 2022-07-08 | 中国科学院水生生物研究所 | Application of galanthamine in preventing and treating fish enteritis caused by bean pulp feed |
| CN109247270A (en) * | 2018-08-07 | 2019-01-22 | 广东省实验动物监测所 | A kind of method of quick screening small test fish building pancreas fatty infiltration model |
Also Published As
| Publication number | Publication date |
|---|---|
| FR3050615A1 (en) | 2017-11-03 |
| CN106386591B (en) | 2019-03-19 |
| FR3050615B1 (en) | 2023-01-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104799124B (en) | A kind of colored clam mixed feed | |
| CN108077594A (en) | It is a kind of to be used to improve red claw crayfish mixed feed of immunity and preparation method thereof | |
| CN103202408B (en) | Environment-friendly nutritional type compound feed of mullet | |
| CN106386591A (en) | Method for establishing erucic acid induced fish enteritis model | |
| CN113508766B (en) | Method for domesticating and feeding largemouth bass fry | |
| CN101156650A (en) | Ricefield eel scrod swelling particle mixed feed | |
| CN106616053A (en) | Aquaculture feed as well as preparation method and application thereof | |
| CN103404466B (en) | Ecological cultivation mode capable of simulating river-sea migration of Yangtze coilianasus | |
| CN103478446A (en) | Compound feed used in penaeus vannamei farming earlier stage | |
| CN104206822B (en) | Efficient compound attractant growth promoting additive and preparation and application thereof | |
| CN103004660A (en) | Parent fish culturing method for scaled artificial breeding of finless ell | |
| CN103931950B (en) | Special abalone sheet feed stuff of a kind of cage and preparation method thereof | |
| CN103549185B (en) | Artificial microparticle feed for clam worm larva and preparation method thereof | |
| CN103478450A (en) | Compound feed used in penaeus vannamei farming later stage | |
| CN101156651A (en) | Ricefield eel postlarva swelling particle mixed feed | |
| CN100372476C (en) | Chinese herbal phagostimulant for paralichthys olivaceus | |
| CN106386590A (en) | Method for establishing gossypol induced fish enteritis model | |
| CN106106274A (en) | A kind of pollution-free breeding method of Lobster | |
| CN111194707A (en) | Efficient outdoor ecological pond artificial breeding method for red sea perch | |
| CN103549187B (en) | Nutrient enrichment feed for parent clam worms and preparation method thereof | |
| CN105831482A (en) | Feed for promoting digestive system growth of grouper larvae | |
| CN107581410A (en) | It is a kind of instead of shrimp feed of fish meal and its preparation method and application | |
| CN107874025A (en) | It is a kind of to improve grass carp, the mixed feed and preparation method thereof of crucian build, and using mixed fodder made of the mixed feed | |
| CN106721658A (en) | Soft pellet diet that a kind of red cream crabs are cultivated and preparation method thereof | |
| CN105961257A (en) | Crawfish and whitefish mixed breeding method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |