CN106377567A - Extraction method for small molecule active substances of traditional Chinese medicine material - Google Patents
Extraction method for small molecule active substances of traditional Chinese medicine material Download PDFInfo
- Publication number
- CN106377567A CN106377567A CN201610898941.0A CN201610898941A CN106377567A CN 106377567 A CN106377567 A CN 106377567A CN 201610898941 A CN201610898941 A CN 201610898941A CN 106377567 A CN106377567 A CN 106377567A
- Authority
- CN
- China
- Prior art keywords
- raw material
- enzymolysis
- molecule active
- crude drug
- active substance
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/35—Caprifoliaceae (Honeysuckle family)
- A61K36/355—Lonicera (honeysuckle)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/898—Orchidaceae (Orchid family)
- A61K36/8984—Dendrobium
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
Abstract
The invention discloses an extraction method for small molecule active substances of traditional Chinese medicine materials. Low-temperature biological enzymolysis and a membrane technology are combined to effectively extract the small molecule active substances in three traditional Chinese medicine materials including radix tetrastigme, honeysuckle flower and the stem of dendrobium officinale. The biological enzymolysis is realized by the following steps: adopting biological compound enzyme formed by compounding cellulase, pectinase and papain in a weight ratio of (1 to 3) : (1 to 2) : (1 to 2); performing enzymolysis at the temperature of 40 to 50DEG C for 4 to 6 hours, and keeping stirring during enzymolysis; in addition, concentrating and filtering an enzymolysis product for two times; finally, performing freeze drying or spray drying to obtain a small molecule active substance product of the traditional Chinese medicine material. The extraction method disclosed by the invention is low in enzymolysis temperature and mild in reaction environment; the activity and the purity of the small molecule active substances in the raw materials can be effectively retained, and the raw materials are utilized to the maximum extent; besides, high temperature is avoided in the whole extraction process; water resources generated after extracting can be recycled, and energy consumption is low.
Description
Technical field
The present invention relates in a kind of Chinese crude drug active substance extracting method, specifically a kind of Chinese crude drug small molecule active thing
The extracting method of matter.
Background technology
Chinese medicine is the rarity of Chinese civilization, has important historic significance and succession to be worth.The kind of Chinese herbal medicine
Class is various, shows according to ASSOCIATE STATISTICS, more than more than 7000 kinds, and is distributed widely in each province of China.Chinese working people
For thousands of years during combating the disease, by practice, constantly recognize, gradually have accumulated abundant knowledge of TCM.
From《Dragon Lord book on Chinese herbal medicine warp》Arrive《Compendium of Materia Medica》, the more various Chinese medicine ancient books and records to the present age, invariably show wisdom and the duty of compatriots
Labor.A large amount of biological active agents, such as saccharide, aminoacid, protein, oils and fatss, wax, flavone, dimension life are contained in Chinese herbal medicine
Element, organic acid, tannin, inorganic salt, volatile oil, alkaloid, glycoside etc., and the medicinal function of heterogeneity has difference.With
Continuous renewal and the development of Medical Technology, traditional Chinese medicine and pharmacy cannot meet the need that the mankind seek Action mechanism further
Ask.In recent years, the report with regard to Chinese herbal medicine small molecule composition occurred repeatedly, and this carries for the innovative research of China's Chinese medicine and pharmacy
New approaches are supplied.The research of Traditional Chinese Herb extract all has original performance in medical domains such as antitumor, malarias, and
Correlational study content obtains the consistent affirmative of international scientific man.Using phytochemical correlation technique, little point is carried out to Chinese medicine
Subconstiuent is analyzed, and carries out biological function research in conjunction with biology techniques to it, is the break-through point of current Development of Chinese Medicine.
Radix Apioris Fortunei (Radix Lespedezae Buergeri) is the exclusive rare medicinal endangered plants of China, is mainly used as medicine with tuber (also known as Radix Tetrastigmatis Hemsleyani), cool in nature, no
Poison, sweet and slightly bitter taste, have the effect such as heat-clearing and toxic substances removing, dispelling pathogenic wind and eliminating phlegm, promoting blood circulation and stopping pain, regulating qi-flowing for strengthening spleen.Clinical taking orally can treat children
Hyperpyrexia convulsion, dysentery, bronchitis, pneumonia, pharyngolaryngitis, hepatitis and viral meningitises, the tuberculosis of cervical lymph nodes, menoxenia and pain
Through etc..Additionally, research shows, it also has to hematopathy and cardiovascular and cerebrovascular disease, infectious disease, rheumatic arthritis gynaecopathia
Certain curative effect.Flos Lonicerae is motherland's tradition medication, has functions that heat-clearing and toxic substances removing, cool breeze radiating, antiviral, hepatic cholagogic, faces
Bed is used for treating multiple disease such as respiratory tract infection, skin infection, dysentery, diseases caused by exogenous pathogenic factor, calentura, headache, pharyngalgia.Flos Lonicerae not only has remarkably
Medical effect, also remarkable health care.Flos Lonicerae is medicinal economical plant environmental with water guarantor, enjoys " national treasure one
The good reputation of branch flower ".Herba Dendrobii is the important traditional Chinese medicine of China, and its medical value was praised highly by the successive dynasties.Most of distribution
In Southwestern China, south China, Taiwan and other places, there are many cultivation bases south China and East China, are many especially with Zhejiang Province.Medicinal iron sheet
Herba Dendrobii mainly contains the various active composition such as polysaccharide, alkaloid, has multiple work(such as nourishing YIN and clearing away heat, the stomach reinforcing that promotes the production of body fluid, nourishing the lung to arrest cough
Effect.Modern pharmacological research shows that it also has raising immunity of organisms, antitumor and prevents and treats the functions such as cataract.And, iron sheet
Herba Dendrobii is the top grade of Dendrobium, ranks " Chinese nine big Herba mesonae chinensis " they because of its special living environment and distinguished nourishing effects
Head, has larger medical value.
However, Radix Apioris Fortunei (Radix Lespedezae Buergeri), Flos Lonicerae, Herba Dendrobii more limit in the use of Chinese Traditional Medicine, mainly with water extract
The treatment of form involved in diseases, it is less that this makes people that the biologic activity of effective ingredient various contained by it is understood.In recent years,
Going deep into Chinese medicine research, a large amount of scholars launch to extract detached research to the micromolecular compound of these three Chinese medicines, according to
Studies have reported that it is known that：(1) in Radix Apioris Fortunei (Radix Lespedezae Buergeri), active small molecular composition mainly has flavonoid, triterpene and steroid, fatty acid
Compound etc.；(2) in Flos Lonicerae, active small molecular composition mainly has volatile oil, flavonoid, organic acid, triterpeness, cyclenes
Ether terpenoid etc.；(3) in Herba Dendrobii active small molecular based on alkaloid, aminoacid takes second place.These three Chinese medicine small moleculars become
Divide and play critical effect in playing its pharmacological function, be also its main bioactive ingredients simultaneously.Regrettably, right
Present Research in these Chinese medicine small moleculars is：Content is few, and level is shallow, and mechanism is unintelligible, and application is not high, Er Qiesuo
Use more chemicals with extracting method, seriously polluted.And cannot avoid be this three classes Chinese medicine small molecular composition medicine
Can relate to multiple biomedical sectors hard, have huge potentiality and market, such as it is in immunology, enteric microorganism group
, oncology and angiocardiology, the even aspect such as health care of food all has larger using value.
Chinese Traditional Medicine to Radix Apioris Fortunei (Radix Lespedezae Buergeri), Flos Lonicerae, Herba Dendrobii utilization, (100 DEG C, 2～3 hours) are hotted plate with high temperature
Based on water extract form, the yield of water extract small molecular material is low.At present, in the industry to Radix Apioris Fortunei (Radix Lespedezae Buergeri), Flos Lonicerae, Herba Dendrobii this
The research of three kinds of Chinese crude drug medicinal functions is concentrated mainly on water extract and bigger molecule, in the biological activity of micromolecular compound
Shortcoming is also compared in the research of screening and its associated biomolecule functional mechanism, and this cannot adapt to and meet the development of current Chinese medicine and pharmacy
Trend.Therefore, develop the extracting method of effective Chinese crude drug small-molecule active substance, in depth research small molecule is extracted
The medicinal function of thing, not only can help us more clearly to understand its Action mechanism, the used time also " steps the gateway of a country, enters into west for it
Pharmacy " lays the first stone, and this meets the policy of national Chinese medicine and pharmacy strategic development, meets the demand of Medical development, and has
Huge using value and market value.For these reasons, the present invention proposes a kind of carrying of Chinese crude drug small-molecule active substance
Take method.
Content of the invention
The technical problem to be solved is：A kind of extracting method of Chinese crude drug small-molecule active substance is provided, should
Extracting method can be effectively retained activity and the purity of the small-molecule active substance in raw material, in addition sharp to raw material to greatest extent
With, and do not use high temperature in whole extraction process, and the water resource reusable edible producing after extraction, energy consumption is low.
The present invention solves the technical scheme that adopted of above-mentioned technical problem：The carrying of a kind of Chinese crude drug small-molecule active substance
Take method, comprise the following steps：
(1) raw material screening：It is some that screening prepares fresh Radix Apioris Fortunei (Radix Lespedezae Buergeri), Flos Lonicerae and Herba Dendrobii raw material；
(2) raw material cleaning：Wash off raw material appearance silt with tap water；
(3) low temperature enzymolysis：Radix Apioris Fortunei (Radix Lespedezae Buergeri) after cleaning, Flos Lonicerae and Herba Dendrobii raw material are put into enzymatic vessel, then to enzyme
Add water in solution tank, the addition of water and the weight of raw material ratio is for 50～100:1, it is heated up to 40～50 DEG C, add raw material weight
0.1～0.5% compound bio-enzyme, this compound bio-enzyme is by cellulase, pectase and carase with 1～3:1～2:1～2
Weight form than compounding；After adding compound bio-enzyme, digest 4～6 hours at a temperature of 40～50 DEG C, keep while enzymolysis
Stirring, obtains enzymatic hydrolysate after enzymolysis；
(4) centrifugation：At a temperature of 40～50 DEG C, with the centrifugation speed of 15000～20000 turns/hour in centrifuge
Degree is centrifuged to enzymatic hydrolysate, removes supernatant after centrifugation, obtains being centrifuged product；
(5) first time thickening filtration：First is carried out to the ultrafilter membrane that centrifugation product molecular cut off is 10000 dalton
Secondary thickening filtration, separation and Extraction lower clear liquid；
(6) second thickening filtration：The lower clear liquid molecular cut off that first time thickening filtration is obtained is 100～500
The NF membrane of dalton carries out second thickening filtration, separation and Extraction supernatant；
(7) it is dried：Lyophilization or spray drying are carried out to the supernatant that second thickening filtration obtains, that is, obtains Chinese medicine
Material small-molecule active substance product.
Preferably, described compound bio-enzyme by cellulase, pectase and carase with 1:1:1 weight is than compounding
Form.
Preferably, the weight of Radix Apioris Fortunei (Radix Lespedezae Buergeri), Flos Lonicerae and Herba Dendrobii is than for 1～5 in step (1):1～2:1～5.
Preferably, raw materials used tuber for fresh Radix Apioris Fortunei (Radix Lespedezae Buergeri), the petal of Flos Lonicerae and Herba Dendrobii in step (1)
Gold bar.
Compared with prior art, it is an advantage of the current invention that：A kind of Chinese crude drug small-molecule active substance that the present invention provides
Extracting method, by low-temperature biological enzymolysis combine to these three Chinese medicines in Radix Apioris Fortunei (Radix Lespedezae Buergeri), Flos Lonicerae and Herba Dendrobii with membrane technology
Small-molecule active substance in material is effectively extracted.Biological enzymolysis adopt by cellulase, pectase and carase with 1～3:
1～2:1～2 weight, than compounding compound bio-enzyme, digests 4～6 hours, while enzymolysis at a temperature of 40～50 DEG C
It is kept stirring for, and thickening filtration twice is carried out to enzymatic hydrolysate, it is little that finally freeze-dried or spray drying obtains Chinese crude drug
Molecular activity physical product.The hydrolysis temperature of extracting method of the present invention is low, and reaction environment is gentle, can be effectively retained little in raw material
The activity of molecular activity material and purity, are used to raw material to greatest extent, and do not use in whole extraction process
High temperature, the water resource reusable edible producing after extraction, energy consumption is low.
Brief description
Fig. 1 is the chromatogram of the Chinese crude drug small-molecule active substance of embodiment 1；
Fig. 2 hots plate the chromatogram of the extract obtaining for high temperature.
Specific embodiment
Below in conjunction with accompanying drawing embodiment, the present invention is described in further detail.
The extracting method of the Chinese crude drug small-molecule active substance of embodiment 1, comprises the following steps：
(1) raw material screening：It is some that screening prepares fresh Radix Apioris Fortunei (Radix Lespedezae Buergeri), Flos Lonicerae and Herba Dendrobii raw material；
(2) raw material cleaning：Wash off raw material appearance silt with tap water；
(3) low temperature enzymolysis：Radix Apioris Fortunei (Radix Lespedezae Buergeri) after cleaning, Flos Lonicerae and Herba Dendrobii raw material are put into enzymatic vessel, then to enzyme
Add water in solution tank, the addition of water and the weight of raw material ratio is for 100:1, it is heated up to 45 DEG C, add the life of raw material weight 0.3%
Thing compound enzyme, this compound bio-enzyme is by cellulase, pectase and carase with 1:1:1 weight forms than compounding；Add life
After thing compound enzyme, digest 6 hours at a temperature of 45 DEG C, be kept stirring for while enzymolysis, after enzymolysis, obtain enzymatic hydrolysate；
(4) centrifugation：At a temperature of 45 DEG C, with the centrifugal speed of 20000 turns/hour to enzymatic hydrolysate in centrifuge
It is centrifuged, after centrifugation, removes supernatant, obtain being centrifuged product；
(5) first time thickening filtration：First is carried out to the ultrafilter membrane that centrifugation product molecular cut off is 10000 dalton
Secondary thickening filtration, separation and Extraction lower clear liquid；
(6) second thickening filtration：The lower clear liquid molecular cut off that first time thickening filtration is obtained is 100 dalton
NF membrane carry out second thickening filtration, separation and Extraction supernatant；
(7) it is dried：Lyophilization or spray drying are carried out to the supernatant that second thickening filtration obtains, that is, is implemented
The Chinese crude drug small-molecule active substance product of example 1.
The extracting method of the Chinese crude drug small-molecule active substance of embodiment 2, comprises the following steps：
(1) raw material screening：It is some that screening prepares fresh Radix Apioris Fortunei (Radix Lespedezae Buergeri), Flos Lonicerae and Herba Dendrobii raw material；
(2) raw material cleaning：Wash off raw material appearance silt with tap water；
(3) low temperature enzymolysis：Radix Apioris Fortunei (Radix Lespedezae Buergeri) after cleaning, Flos Lonicerae and Herba Dendrobii raw material are put into enzymatic vessel, then to enzyme
Add water in solution tank, the addition of water and the weight of raw material ratio is for 80:1, it is heated up to 50 DEG C, add the life of raw material weight 0.5%
Thing compound enzyme, this compound bio-enzyme is by cellulase, pectase and carase with 1:2:2 weight forms than compounding；Add life
After thing compound enzyme, digest 4 hours at a temperature of 50 DEG C, be kept stirring for while enzymolysis, after enzymolysis, obtain enzymatic hydrolysate；
(4) centrifugation：At a temperature of 40～50 DEG C, with the centrifugal speed of 15000 turns/hour to enzymolysis in centrifuge
Product is centrifuged, and removes supernatant after centrifugation, obtains being centrifuged product；
(5) first time thickening filtration：First is carried out to the ultrafilter membrane that centrifugation product molecular cut off is 10000 dalton
Secondary thickening filtration, separation and Extraction lower clear liquid；
(6) second thickening filtration：The lower clear liquid molecular cut off that first time thickening filtration is obtained is 300 dalton
NF membrane carry out second thickening filtration, separation and Extraction supernatant；
(7) it is dried：Lyophilization or spray drying are carried out to the supernatant that second thickening filtration obtains, that is, is implemented
The Chinese crude drug small-molecule active substance product of example 2.
The extracting method of the Chinese crude drug small-molecule active substance of embodiment 3, comprises the following steps：
(1) raw material screening：It is some that screening prepares fresh Radix Apioris Fortunei (Radix Lespedezae Buergeri), Flos Lonicerae and Herba Dendrobii raw material；
(2) raw material cleaning：Wash off raw material appearance silt with tap water；
(3) low temperature enzymolysis：Radix Apioris Fortunei (Radix Lespedezae Buergeri) after cleaning, Flos Lonicerae and Herba Dendrobii raw material are put into enzymatic vessel, then to enzyme
Add water in solution tank, the addition of water and the weight of raw material ratio is for 50:1, it is heated up to 40 DEG C, add the life of raw material weight 0.24%
Thing compound enzyme, this compound bio-enzyme is by cellulase, pectase and carase with 3:2:1 weight forms than compounding；Add life
After thing compound enzyme, digest 5 hours at a temperature of 40 DEG C, be kept stirring for while enzymolysis, after enzymolysis, obtain enzymatic hydrolysate；
(4) centrifugation：At a temperature of 40 DEG C, with the centrifugal speed of 18000 turns/hour to enzymatic hydrolysate in centrifuge
It is centrifuged, after centrifugation, removes supernatant, obtain being centrifuged product；
(5) first time thickening filtration：First is carried out to the ultrafilter membrane that centrifugation product molecular cut off is 10000 dalton
Secondary thickening filtration, separation and Extraction lower clear liquid；
(6) second thickening filtration：The lower clear liquid molecular cut off that first time thickening filtration is obtained is 400 dalton
NF membrane carry out second thickening filtration, separation and Extraction supernatant；
(7) it is dried：Lyophilization or spray drying are carried out to the supernatant that second thickening filtration obtains, that is, is implemented
The Chinese crude drug small-molecule active substance product of example 3.
For the Chinese crude drug small-molecule active substance product of embodiment 1, its molecular weight is detected by high performance liquid chromatograph
Distribution, the chromatogram obtaining is shown in Fig. 1, and molecular weight detection the results are shown in Table 1.Chinese Traditional Medicine is to Radix Apioris Fortunei (Radix Lespedezae Buergeri), Flos Lonicerae, Herba Dendrobii
Utilization, in the form of the water extract based on, accordingly, as contrast, (100 DEG C, 2～3 hours) Radix Apioris Fortunei (Radix Lespedezae Buergeri), gold are hotted plate for high temperature
The dried product of concentrated solution after Flos Lonicerae, Herba Dendrobii, similarly detects its molecular weight distribution by high performance liquid chromatograph,
The chromatogram obtaining is shown in Fig. 2, and molecular weight detection the results are shown in Table 2.
The molecular weight detection result of the Chinese crude drug small-molecule active substance product of table 1 embodiment 1
| Peak area percent (%) | Number-average molecular weight | Weight average molecular weight | |
| Peak 1 | 0.47 | 11796 | 14633 |
| Peak 2 | 43.03 | 727 | 867 |
| Peak 3 | 56.50 | / | / |
Table 2 high temperature hots plate the molecular weight detection knot of Radix Apioris Fortunei (Radix Lespedezae Buergeri), Flos Lonicerae, the dried product of concentrated solution after Herba Dendrobii
Really
| Peak area percent (%) | Number-average molecular weight | Weight average molecular weight | |
| Peak 1 | 83.83 | 1397 | 1923 |
| Peak 2 | 16.17 | / | / |
Contrast Tables 1 and 2 is visible, for the yield of small-molecule substance, the Chinese crude drug small-molecule active substance of embodiment 1
Product middle-molecular-weihydroxyethyl be the weight of the active substance of 727 dalton than for 43.03%, work below 727 dalton for the molecular weight
The weight of property material is than for 99.53%；And the water extract middle-molecular-weihydroxyethyl that conventional high-temperature is hotted plate is the active substance of 1397 dalton
Weight than for 83.83%.It can be seen that, the yield of the Chinese crude drug small-molecule active substance that extracting method of the present invention obtains is higher, point
Son amount is less.
Claims (4)
1. a kind of extracting method of Chinese crude drug small-molecule active substance is it is characterised in that comprise the following steps：
（1）Raw material screening：It is some that screening prepares fresh Radix Apioris Fortunei (Radix Lespedezae Buergeri), Flos Lonicerae and Herba Dendrobii raw material；
（2）Raw material cleans：Wash off raw material appearance silt with tap water；
（3）Low temperature digests：Radix Apioris Fortunei (Radix Lespedezae Buergeri) after cleaning, Flos Lonicerae and Herba Dendrobii raw material are put into enzymatic vessel, then to enzymatic vessel
In add water, the addition of water and the weight of raw material are than for 50～100:1, be heated up to 40～50 DEG C, add raw material weight 0.1～
0.5% compound bio-enzyme, this compound bio-enzyme is by cellulase, pectase and carase with 1～3:1～2:1～2 weight
Form than compounding；After adding compound bio-enzyme, digest 4～6 hours at a temperature of 40～50 DEG C, be kept stirring for while enzymolysis,
Enzymatic hydrolysate is obtained after enzymolysis；
（4）Centrifugation：At a temperature of 40～50 DEG C, with the centrifugal speed pair of 15000～20000 turns/hour in centrifuge
Enzymatic hydrolysate is centrifuged, and removes supernatant after centrifugation, obtains being centrifuged product；
（5）First time thickening filtration：The ultrafilter membrane that centrifugation product molecular cut off is 10000 dalton is carried out dense for the first time
Contracting is filtered, separation and Extraction lower clear liquid；
（6）Second thickening filtration：The lower clear liquid molecular cut off that first time thickening filtration is obtained is 100～500 dongle
The NF membrane paused carries out second thickening filtration, separation and Extraction supernatant；
（7）It is dried：Lyophilization or spray drying are carried out to the supernatant that second thickening filtration obtains, that is, obtains Chinese crude drug little
Molecular activity physical product.
2. a kind of extracting method of Chinese crude drug small-molecule active substance according to claim 1 is it is characterised in that described
Compound bio-enzyme is by cellulase, pectase and carase with 1:1:1 weight forms than compounding.
3. a kind of extracting method of Chinese crude drug small-molecule active substance according to claim 1 is it is characterised in that step（1）
The weight of middle Radix Apioris Fortunei (Radix Lespedezae Buergeri), Flos Lonicerae and Herba Dendrobii is than for 1～5:1～2:1～5.
4. the extracting method of a kind of Chinese crude drug small-molecule active substance according to any one of claim 1-3, its feature
It is step（1）In raw materials used tuber, the petal of Flos Lonicerae and Herba Dendrobii for fresh Radix Apioris Fortunei (Radix Lespedezae Buergeri) gold bar.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610898941.0A CN106377567A (en) | 2016-10-14 | 2016-10-14 | Extraction method for small molecule active substances of traditional Chinese medicine material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610898941.0A CN106377567A (en) | 2016-10-14 | 2016-10-14 | Extraction method for small molecule active substances of traditional Chinese medicine material |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106377567A true CN106377567A (en) | 2017-02-08 |
Family
ID=57937372
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610898941.0A Withdrawn CN106377567A (en) | 2016-10-14 | 2016-10-14 | Extraction method for small molecule active substances of traditional Chinese medicine material |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106377567A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107446978A (en) * | 2017-08-15 | 2017-12-08 | 陈玫辛 | A kind of stem of noble dendrobium small-molecular peptides and preparation method thereof |
| CN110522851A (en) * | 2019-07-11 | 2019-12-03 | 邓少东 | A kind of Chinese medicine composition for treating cancer in digestive system |
| JP2019214543A (en) * | 2018-06-14 | 2019-12-19 | 科技研株式会社 | Crude drug active principle natural extraction method by living matter catalytic activity of plants |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103435486A (en) * | 2013-09-12 | 2013-12-11 | 南京泽朗农业发展有限公司 | Novel method for preparing high-purity chlorogenic acid in honeysuckle |
| CN104311688A (en) * | 2014-11-10 | 2015-01-28 | 安徽大学 | Extraction and separation method for dendrobium nobile polyose and dendrobium nobile total alkali |
-
2016
- 2016-10-14 CN CN201610898941.0A patent/CN106377567A/en not_active Withdrawn
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103435486A (en) * | 2013-09-12 | 2013-12-11 | 南京泽朗农业发展有限公司 | Novel method for preparing high-purity chlorogenic acid in honeysuckle |
| CN104311688A (en) * | 2014-11-10 | 2015-01-28 | 安徽大学 | Extraction and separation method for dendrobium nobile polyose and dendrobium nobile total alkali |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107446978A (en) * | 2017-08-15 | 2017-12-08 | 陈玫辛 | A kind of stem of noble dendrobium small-molecular peptides and preparation method thereof |
| JP2019214543A (en) * | 2018-06-14 | 2019-12-19 | 科技研株式会社 | Crude drug active principle natural extraction method by living matter catalytic activity of plants |
| CN110522851A (en) * | 2019-07-11 | 2019-12-03 | 邓少东 | A kind of Chinese medicine composition for treating cancer in digestive system |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103039671B (en) | Chinese caterpillar fungus instant tea | |
| CN101385829B (en) | Fresh gastrodia tuber full powder and preparation technique thereof | |
| CN104256584A (en) | Moringa oleifera ferment and preparation method thereof | |
| CN107595893A (en) | A kind of enzyme treated prepared slices of Chinese crude drugs and preparation method thereof | |
| CN106377567A (en) | Extraction method for small molecule active substances of traditional Chinese medicine material | |
| KR20130011511A (en) | Fermented ginseng food manufacturing method | |
| CN104357332B (en) | Aspergillus niger JH-2 and application to biotransformation and synthesis of asiatic acid | |
| CN106107345A (en) | A kind of method of Semen Ziziphi Spinosae fermentation preparation enzyme beverage | |
| CN103301321B (en) | Thrombolytic active polysaccharide mixture preparation technology | |
| CN105685620A (en) | Moringa oleifera oral liquid and preparation method thereof | |
| CN105055243A (en) | Hypericum japonicum shower gel capable of clearing heat and promoting dieresis and preparation method thereof | |
| CN105998222A (en) | Probiotic fermented unprocessed common monkshood daughter root composition and preparation method and application thereof | |
| CN106994172A (en) | A kind of Chinese medicine composition of prophylactic treatment gut of shrimp microsporidian, pulvis and preparation method thereof | |
| CN106539085A (en) | A kind of health food with lowering blood pressure and blood fat | |
| CN105796656A (en) | Cistanche tubulosa extract and preparation method thereof | |
| KR101093006B1 (en) | Method for producing functional health food containing bezoar bovis used microbes and the functional health food produced by the same | |
| KR20150104406A (en) | Method of extracting acanthopanax senticosus stem having an effect of increased | |
| CN104739913A (en) | Preparation for accelerating wound healing, and preparation method and application thereof | |
| CN109876053A (en) | A kind of acne-removing inflammation-diminishing, removing toxic substances of clearing liver prescription and its preparation method | |
| CN109329671A (en) | A kind of edible drink product and preparation method thereof for treating gout | |
| CN102746418B (en) | Sansevieria trifasciata polysaccharides as well as preparation method and application thereof | |
| CN103550433A (en) | Extraction method of saffron crocus essence substances | |
| CN104055184B (en) | Hirsutella sinensis hypolipidemic healthcare beverage and preparation method thereof | |
| CN110613841B (en) | Snailase traditional Chinese medicine ointment for treating psoriasis and preparation method thereof | |
| CN107468998A (en) | A kind of Chinese hawthorn blood-pressure-reducing dip and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20170214 Address after: 315613 Ninghai County in Zhejiang province Ningbo City West Dian Zhen Shi Kong Village No. 409 Applicant after: Feng Yu Applicant after: Zhejiang Shuangyu biological Limited by Share Ltd Applicant after: Ningbo Bofeng Biotechnology Co., Ltd. Address before: Five Ninghai County, Zhejiang province 315613 Ningbo City West Zhen Binhai Industrial Zone, Road No. 12 Applicant before: Zhejiang Shuangyu biological Limited by Share Ltd Applicant before: Ningbo Bofeng Biotechnology Co., Ltd. |
|
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20170208 |