KR20130011511A - Fermented ginseng food manufacturing method - Google Patents
Fermented ginseng food manufacturing method Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
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- A—HUMAN NECESSITIES
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- A61K39/39—Medicinal preparations containing antigens or antibodies characterised by the immunostimulating additives, e.g. chemical adjuvants
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
- A23V2250/20—Natural extracts
- A23V2250/21—Plant extracts
- A23V2250/2124—Ginseng
Abstract
Description
본 발명은 고등균류를 인삼에 접종하여 40일간 배양시킨 발효삼 배양물을 이용하여 제조한 면역증진용 식품의 제조방법에 관한 것이다.The present invention relates to a method for producing food for immuno-promoting food prepared by fermenting ginseng culture inoculated with higher fungi in ginseng and cultured for 40 days.
신비의 영약으로 알려진 고려인삼(Panax ginseng C.A. Meyer)은 오갈피나무과(Araliceae) 인삼속(Panax)에 속하는 다년생 초본류로서, 한방에서는 그 뿌리를 인삼(Ginseng radix)이라 하여 약용으로 사용하고 있다.
Korean ginseng (Panax ginseng CA Meyer), known as the mysterious elixir, is a perennial herb belonging to the genus Araliceae Panax ginseng, and its root is called ginseng (Ginseng radix) for medicinal purposes.
인삼이 언제부터 약용으로 사용되었는지 정확히 알려져 있지 않으나, 약 2000년전 고대 중국의 전한시대(前漢時代) 사유(史遊)가 저술한 급취장(急就章)에 인삼에 대한 기록이 있고, 중국의 최고 본초서인 신농본초경(神農本草經, A.D. 456~536)에 인삼의 효능에 대하여 비교적 상세하고 구체적으로 기술되어 있다.
Although it is not known exactly when ginseng has been used for medicinal purposes, there is a record of ginseng on the oysters written by the history of the ancient Chinese era of ancient China about 2000 years ago. Seoin Sinnonbonchocho (神 本 本草 經, AD 456-536) describes the efficacy of ginseng relatively detailed and specific.
신농본초경에는 인삼은 오장 즉, 간장, 심장, 폐장, 신장, 비장의 양기를 돋구어 주는 주약으로 사용하고, 위장의 기를 열어주며, 곽란, 구토, 갈증을 멎게 하고 혈액을 잘 통하게 하며 오래 복용하면 몸이 가벼워지고 장수한다고 기록하고 있다. 또한 인삼의 주요성분인 사포닌은 암, 당뇨병, 남성기능 향상, 고혈압, 동맥경화, 치매, 골다공증 예방, 노화방지, 두뇌활동 촉진, 갱년기 장애, 여성 피부미용 등에 좋은 것으로 알려져 있다. 특히 항암제보다 더 높은 항암작용을 하고 강력한 혈당강하작용을 하며 합병증을 억제시키는 효능이 있는 것으로 밝혀졌다.
Ginseng is used as a medicine to boost the yang of the liver, heart, lungs, kidneys, and spleen, and it opens the gastrointestinal tract, relieves ovulation, vomiting, thirst, and keeps blood flowing well. It is lighter and longer life. In addition, saponin, a major component of ginseng, is known to be good for cancer, diabetes, male function enhancement, hypertension, arteriosclerosis, dementia, osteoporosis prevention, anti-aging, brain activity promotion, menopausal disorders, and skin care for women. In particular, it has been shown to have higher anticancer activity, potent hypoglycemic effect and anti-complications than anticancer drugs.
인삼의 주요성분인 사포닌의 화학구조는 크게 당부분(glycone)과 비당부분(aglycone)으로 구성된 배당체이다. 이러한 사포닌이 체내에서 흡수될 때는 사람의 장내에 있는 특유 미생물에 의해 분해되어 체내로 흡수되어진다. 하지만 인삼사포닌을 분해하는 장내 미생물은 사람의 체질에 따라 그리고 식습관에 따라 그 존재의 유무와 보유하고 있는 정도가 다르며, 그로 인해 임삼의 복용 후 저마다 효능의 차이가 나타날 수 있다.
The chemical structure of saponin, the main component of ginseng, is a glycoside consisting of glyco and aglycone. When these saponins are absorbed by the body, they are broken down by the unique microorganisms in the human intestine and absorbed into the body. However, the intestinal microorganisms that break down ginseng saponin vary depending on the constitution and dietary habits of people, and their existence and degree of retention are different.
한편, 고등균류인 영지버섯(Ganoderma lucidum)은 불로초라고도 한다. 여름에 활엽수 뿌리에서 발생하여 땅 위에도 돋는다. 갓과 자루 표면에 옻칠을 한 것과 같은 광택이 있는 1년생 버섯이다. 한방에서는 강장 진해 소종(消腫) 등의 효능이 있어 신경쇠약 심장병 고혈압 각종 암종에 사용한다. 이 버섯은 갓자루가 단단한 각피로 싸여 있고 니스를 칠한 것 같은 광택이 있어 일본에서는 만년버섯, 중국에서는 영지라고 하여 한약 재료로 귀하게 사용하고 장식용으로도 이용되며, 세계적으로 널리 분포한다.
Meanwhile, Ganoderma , a higher fungus, Ganoderma lucidum lucidum ) is also called bullocho. It occurs in the roots of hardwoods in summer and also sprouts on the ground. It is a year-round mushroom that has a gloss like lacquer on its lampshade and bag surface. In oriental medicine, tonic jinhae swelling (消腫) is effective, such as neural breakdown heart disease hypertension used in various cancers. These mushrooms are covered with hard skin and have a varnish like varnish. They are used as an herbal medicine in Japan, as a mushroom in Japan, and as a territory in China, and are widely used for decorative purposes.
본 발명자는 인삼, 고등균류가 가지는 면역증강 효과를 활용하여 인삼에 고등균류를 배양시켜 고등균류가 지니고 있는 생물학적 변환능력에 의해 발효삼이 가지고 있는 면역증강 효과가 현저히 증가됨을 확인하고 본 발명을 완성하였다. The present inventors have completed the present invention by culturing higher fungi in ginseng by using the immune enhancing effect of ginseng and higher fungi, thereby significantly increasing the immune enhancing effect of fermented ginseng by the biological conversion ability of the higher fungi. .
본 발명의 목적은 인삼에 고등균류를 발효시켜 인삼의 유효성분을 사람의 체질과 상관없이 용이하게 섭취할 수 있고, 인삼과 고등균류가 지니고 있는 면역효과를 극대화시킬 수 있는 발효삼 식품을 제조하는데 있다.It is an object of the present invention to ferment higher fungi in ginseng to easily ingest the active ingredient of ginseng irrespective of the human constitution, and to prepare a fermented ginseng food that can maximize the immune effect of ginseng and higher fungi have.
상기 과제를 해결하기 위한 본 발명에 따른 면역증강용 식품은 인삼에 고등균류를 발효시킨 발효삼 추출물을 포함한다.
Immunity-enhancing foods according to the present invention for solving the above problems include fermented ginseng extract fermented with higher fungi in ginseng.
본 발명에 따른 면역증강용 발효삼 식품의 제조방법은 인삼을 건조하는 단계; 상기 인삼을 절삼하는 단계; 상기 인삼을 멸균하는 단계; 상기 인삼과 고등균류를 접종하는 단계; 상기 인삼과 고등균류를 발효시켜 발효물을 만드는 단계; 상기 발효물을 동결건조하는 단계; 상기 건조된 발효물 및 백복령, 당귀, 천궁, 숙지황, 백작약 등의 약재와 함께 추출하는 단계; 상기의 추출물을 농축하는 단계; 상기의 농축액에 저감미당의 첨가료를 넣어 식품 조성을 만드는 단계를 포함 한다.Method for producing a fermented ginseng food for immunological enhancement according to the present invention comprises the steps of drying the ginseng; Cutting off the ginseng; Sterilizing the ginseng; Inoculating the ginseng and higher fungi; Fermenting the ginseng and higher fungi to make fermented products; Lyophilizing the fermented product; Extracting the dried fermented products together with medicinal herbs such as Baekbokryeong, Dongguk, Cheonggung, Sukjihwang, and Baekjak; Concentrating the extract; Including the additive of the reduced sweetened sugar in the concentrate comprises the step of making a food composition.
상기 인삼을 수세한 후 건조하는 단계는 상온에서 수삼표면의 수분이 마를 때까지 3~5시간 정도 자연건조 시킨다. After washing the ginseng and drying step is to dry naturally for 3 to 5 hours until the moisture of the surface of the ginseng dries at room temperature.
상기 건조된 인삼을 어슷하게 절삼하여 100~150g씩 배양병에 입병한다.The dried ginseng is severely cut and put into 100-150 g culture bottles.
상기의 인삼을 고압멸균기를 이용해 121℃에서 60분가량 멸균한다.The ginseng is sterilized for about 60 minutes at 121 ℃ using an autoclave.
상기의 인삼에 고등균류 접종량은 인삼 중량의 5~30%의 양으로 접종한다.The inoculation amount of higher fungi in the ginseng is inoculated in an amount of 5-30% of the ginseng weight.
상기 고등균류와 인삼을 발효시켜 발효물을 만드는 단계는 25~30℃에서 30~50일간 고체 발효한다.The fermentation process by fermenting the higher fungi and ginseng is a solid fermentation for 30 to 50 days at 25 ~ 30 ℃.
상기 발효물을 -30~-50℃에서 진공동결건조하여 건조물을 만든다.The fermented product is dried in vacuum freeze drying at -30 ~ -50 ℃.
상기 발효삼 건조물에 백복령, 천궁, 백작약, 당귀, 숙지황과 함께 고압추출기를 이용하여 85℃에서 8~24시간 추출한 후 여과하여 발효삼 추출물을 만든다. 상기 추출물을 감압농축기를 이용하여 고형분 함량이 65%가 되도록 농축한다. 상기 발효삼 및 식물혼합 농축액에 저감미당을 첨가하여 발효삼 식품조성을 만든다.Baekbokyeong, Cheonggung, Baekjak, Dongguk, Sukjihwang extract the fermented ginseng extract by extracting 8 ~ 24 hours at 85 ° C. using a high pressure extractor. The extract is concentrated to a solid content of 65% using a reduced pressure concentrator. Adding fermented ginseng to the fermented ginseng and plant mixture concentrate to make fermented ginseng food composition.
이하, 본 발명을 실시예에 의해 상세히 설명한다.Hereinafter, the present invention will be described in detail by way of examples.
본 발명에서 발효삼 추출물을 함유하는 면역증강용 식품은 인삼에 고등균류를 접종시켜 생체내에 흡수가 가능한 인삼의 사포닌 및 다당체 형태로 미리 만들어줌으로써 대사과정을 통해 생체내의 흡수력을 높여 남녀노소 누구나 인삼 사포닌 및 다당체의 효능이 발휘될 수 있도록 표준화를 할 수 있으며 또한 체내 면역계를 구성하는 면역세포를 활성화시켜 바이러스, 세균 등으로부터의 감염되는 것을 방지하는 효과가 있다.Immunity-enhancing foods containing fermented ginseng extract in the present invention by inoculating higher fungi on ginseng in advance in the form of saponins and polysaccharides of ginseng that can be absorbed in vivo to increase the absorption in vivo through the metabolic process everyone ginseng saponins And it can be standardized so that the efficacy of the polysaccharide can be exerted, and also has the effect of preventing the infection from viruses, bacteria, etc. by activating the immune cells constituting the body's immune system.
도 1은 본 발명에 따른 발효삼 식품(정옥고)의 제조공정을 순차적으로 나타내는 순서도이다.1 is a flow chart sequentially showing the manufacturing process of the fermented ginseng food (jeongokgo) according to the present invention.
이하, 본 발명의 바람직한 실시 예를 설명하기로 한다.
Hereinafter, preferred embodiments of the present invention will be described.
<실시예 1> 발효삼 건조 배양물 Example 1 Fermented Ginseng Dry Culture
수삼을 깨끗한 물로 세척한 후, 상온에서 3~5시간 건조시켜 수삼표면의 수분을 건조시킨 후, 칼을 이용하여 수삼을 0.5cm 두께로 절단하고, 배양병에 120~150g씩 담아 고압멸균기를 이용하여 121℃, 1.2kgf/cm2에서 60분간 멸균한 후 멸균기에서 배양병을 꺼내어 방냉 후 영지버섯 종균을 10% 접종하고, 30℃에서 30~50일간 고체발효시키고, -30~-50℃에서 진공동결건조시켜 발효삼 건조 배양물을 제조하였다.After washing the fresh ginseng with clean water, drying it at room temperature for 3 ~ 5 hours to dry the moisture on the surface of the fresh ginseng, cut the fresh ginseng into 0.5cm thick with a knife, and put 120 ~ 150g each into a culture bottle using an autoclave. Sterilize at 121 ℃, 1.2kgf / cm 2 for 60 minutes, remove the culture bottle from the sterilizer, and after cooling, inoculate 10% of Ganoderma lucidum spawn, and solid fermentation at 30 ℃ for 30-50 days, at -30 ~ -50 ℃ Vacuum freeze-drying to prepare fermented ginseng dry culture.
<실시예 2> 발효삼 농축액 Example 2 Fermented Ginseng Concentrate
실시예 1에서 제조된 발효삼 건조 배양물에 20배수의 정제수를 첨가하여 고압추출기를 이용해 85℃에서 8~24시간 추출한 후 감압농축기를 이용하여 고형분 65%의 발효삼 농축액을 제조한다. 20 times of purified water was added to the fermented ginseng dry culture prepared in Example 1, followed by extraction at 85 ° C. for 8 to 24 hours using a high pressure extractor to prepare fermented ginseng concentrate of 65% solids using a reduced pressure concentrator.
<실시예 3> 식물혼합 추출물 Example 3 Plant Mixture Extract
백복령, 당귀, 천궁, 백작약, 숙지황에 10배수의 정제수를 첨가하여 고압추출기를 이용해 85℃에서 8~24시간 추출하여 고형분 65%의 식물혼합 농축액을 제조한다. Add 10-fold purified water to Baekbokryeong, Dongguk, Cheonggung, Baekjak, Sukjihwang, and extract the plant mixture concentrate of solid content 65% by extracting 8 ~ 24 hours at 85 ℃ using a high pressure extractor.
<실시예 4> 발효삼 식품(정옥고) 조성 Example 4 Fermented Ginseng Food (Jeong Okgo) Composition
상기 발효삼 농축액(고형분 65%) 5%, 홍삼농축액(고형분 65%) 5%, 식물혼합 추출농축액(고형분 65%)50%, 저감미당 40% 합계 100%로 발효삼 식품(정옥고)를 제조한다.
5% fermented ginseng concentrate (65% solids), 5% red ginseng concentrate (65% solids), 50% plant mixture extract concentrate (65% solids), 100% total 40% per reduced rice fermented ginseng food (Jeong Okgo) Manufacture.
<실험예 1> 발효삼 농축액의 진세노사이드 Rg1 +Rb1의 함량Experimental Example 1 Content of Ginsenoside Rg 1 + Rb 1 in Fermented Ginseng Extract
발효삼 농축액 1g을 정밀히 달아 250ml 환류용 플라스크에 취한다. 50% 메탄올 용액 50ml를 가하여 70~80℃ 수욕에서 1시간 환류 냉각한다. 식히고, 원심분리한 다음 상징액을 환저플라스크에 취한다. 잔류물에 대해서 1회 더 반복한다. 환저플라스크에 옮긴 상징액을 수욕중에서 60℃이하에서 감압농축한다. 농축물을 증류수:아세토니트릴 혼합액(80:20) 2ml에 용해한다. 멤브레인 필터(0.45㎛)로 여과하여 시험용액으로 한다. 상기 시험용액을 액체크로마토그래프/자외부흡광광도검출기를 이용하여 최대흡수파장인 203nm에서 정량분석을 한다.1 g of fermented ginseng concentrate is precisely weighed and taken into a 250 ml reflux flask. 50 ml of 50% methanol solution was added, and the mixture was cooled to reflux for 1 hour in a 70-80 ° C water bath. After cooling, centrifugation and the supernatant are taken in a round bottom flask. Repeat once more for the residue. The supernatant transferred to the round bottom flask is concentrated under reduced pressure at 60 ℃ or lower in the water bath. The concentrate is dissolved in 2 ml of a distilled water: acetonitrile mixture (80:20). Filter by membrane filter (0.45㎛) to prepare a test solution. The test solution is quantitatively analyzed at 203 nm, the maximum absorption wavelength, using a liquid chromatograph / ultraviolet absorption detector.
<실험예 2> 식물혼합추출물의 재료조성Experimental Example 2 Material Composition of Plant Mixture Extract
백복령, 당귀, 천궁, 백작약, 숙지황을 각각 추출한 추출액의 관능평가를 통해 표2.와 같이 3종류의 약재배합비율을 정하여 식물혼합추출물을 제조하여 관능테스트를 실시한 결과 sample2의 맛, 향, 색도 등 전체적인 기호도가 우수하여 sample2의 배합비율로 식물혼합추출물의 재료조성을 정하였다.Sensory evaluation of the extracts extracted from Baekbokryeong, Dongguk, Cheonggung, Baekjak, and Sukjihwang, respectively, determined the three kinds of medicinal herb mixture ratios, and produced a mixture of plants. The overall preference was excellent and the material composition of plant mixture extract was determined by the mixing ratio of sample2.
(7점)Overall likelihood
(7 points)
<실험예 3> 발효삼 식품(정옥고)조성Experimental Example 3 Preparation of Fermented Ginseng Food
발효삼 식품(정옥고)의 제조를 위하여 발효삼 추출물 및 식물혼합추출물에 저감미당의 식품첨가료를 첨가하는 3종의 배합비율을 조제하여 관능테스트를 실시하여 발효삼 식품(정옥고)의 최종 조성을 sample 3으로 하여 제조하였다. For the production of fermented ginseng food (Jeong Okgo), three kinds of compounding ratios are added to the fermented ginseng extract and plant mixture extract to add reduced sweetened food additives. The composition was prepared as sample 3.
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