CN106376530B - Prevent the sericulture method of silkworm morbidity - Google Patents
Prevent the sericulture method of silkworm morbidity Download PDFInfo
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- 238000009366 sericulture Methods 0.000 title claims abstract description 18
- 238000000034 method Methods 0.000 title claims abstract description 15
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- 235000008708 Morus alba Nutrition 0.000 claims abstract description 59
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- 230000002265 prevention Effects 0.000 claims description 7
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- 206010029803 Nosocomial infection Diseases 0.000 description 1
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- 230000001524 infective effect Effects 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
- A01K67/04—Silkworms
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/16—Amides, e.g. hydroxamic acids
- A61K31/17—Amides, e.g. hydroxamic acids having the group >N—C(O)—N< or >N—C(S)—N<, e.g. urea, thiourea, carmustine
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
- A61K31/35—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom
- A61K31/352—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom condensed with carbocyclic rings, e.g. methantheline
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
- A61K31/496—Non-condensed piperazines containing further heterocyclic rings, e.g. rifampin, thiothixene or sparfloxacin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
- A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
- A61K31/519—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim ortho- or peri-condensed with heterocyclic rings
- A61K31/52—Purines, e.g. adenine
- A61K31/522—Purines, e.g. adenine having oxo groups directly attached to the heterocyclic ring, e.g. hypoxanthine, guanine, acyclovir
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Epidemiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Environmental Sciences (AREA)
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Abstract
A kind of sericulture method for preventing silkworm to fall ill of the present invention, is used urea, DHPG, posaconazole, siskin isoflavonoid by weight for 950:30:10:10 ratio mixing, then sprayed 2~2.5 kilograms of mulberry leaf with after the dissolving of 200~250ml water by 1.5~2.0 grams of mixtures, 5 age silkworms are fed after drying.The present invention can significantly reduce silkworm blood type pus illness, mind-intesting cytoplasmic polyhedrosis, the morbidity of white muscardine, improve the security bred silkworms, and increase cocoon yield.
Description
Technical field
The invention belongs to agriculture sericulture field, especially in regard to a kind of sericulture method that silkworm can be prevented to fall ill.
Background technology
Influence cocoon yield, the principal element of quality have Silkworm varieties, the mulberry leaf food value of leaf, sericulture technology, generation of silkworm disease etc., right
For a certain specific kind, the food value of leaf of mulberry leaf is to determine the most important factor of cocoon yield.Extreme weather environment is added
Mulberry tree field management, fertilising are not in place, have a strong impact on growing for mulberry tree, cause Quality of Mulberry Leaves to decline, it is impossible to meet silkworm life
Long development, requirement of the silk protein synthesis to nutrition, ultimately result in cocoon yield decline.
In production of breeding silkworms, due to Silkworm varieties resistance, sterilize not thoroughly, the food value of leaf is poor, feeding environment high temperature and humidity with
And many reasons such as pollution by pesticides, being often developed to middle and later periods outburst communicable disease in 5 age silkworms causes cocoon yield to be regretted
Receive, influence silkworm cocoon yield stability.The communicable disease of influence silkworm cocoon yield stability mainly has silkworm blood type pus illness, middle intestines
Type pus illness, desonucleosis, white muscardine, green muscardine etc..
Because silkworm is poikilotherm, environment sensitive to external world, growth cycle is short, without it is similar to higher mammal it is perfect after
Effective protective agents in its immune system, and production of breeding silkworms at present also not for above-mentioned infectiousness silkworm disease, therefore life of breeding silkworms
Above-mentioned infectiousness silkworm disease is prevented and treated in production main using putting prevention first, anti-to overweight the Comprehensive Control Strategy controlled, major measure has (1) feeding
The strong kind of resistance against diseases is supported, (2) carry out thorough disinfection, (3) before, during and after breeding silkworms to silkworm room, rearing instrument and sericulture surrounding enviroment
Strengthen managing of mulberry field, the rational application of fertilizer, improve the food value of leaf, (4) good mulberry is satiated with food, and strengthens and mascardine-proof dust or silkworm are used before silkworm constitution, (5) first feeding
The agent of body disinfection of rearing bed carries out silkworm body surface sterilization, prevents the infection of fungi, (6) remove sand in time, suitably carries green grass or young crops in batches, difference development ranks
The silkworm fed-batch of section, (7) correctly diagnose, eliminate sick silkworm, appropriate dilute foster, reinforcement silkworm body and seat sterilization, prevent silkworm rearing bed from passing in time
Dye, (8) strengthen mulberry field and control worm, prevent cross-infection, the most thermophilic needed for (9) regulation Indoor Micro, guarantee silkworm normal development
Humidity, (10) reduce high/low-temperature impact, prevent that silkworm constitution declines caused by high/low-temperature impact, (11) are prevented in silkworm agricultural chemicals
Poison.
Stable and high yields of these comprehensive preventive health measures to silk cocoon has played positive role.However, because silkworm does not have
Have in the immune system that improves of vertebrate, production also not to virosis, stiff disease resistant improved seeds, silkworm is once
The virus of Infective or fungi, in most cases silkworm is dead because of infection.Therefore, although being taken in production of breeding silkworms
Various comprehensive preventive health measures, but still often largely occur infectiousness silkworms disease in 5 middle and later periods in age, cause cocoon yield to be regretted
Receive, the stability of influence silk cocoon production, and silkworm belongs to oligophagy insect, mainly eat mulberry leaf, and on producing often because of the food value of leaf not
It is good and influence cocoon yield.Therefore breed silkworms in producing in the urgent need to the pathogenetic sericulture side of one kind increase cocoon yield prevention silkworm
Method.
The content of the invention
It is an object of the invention to provide it is a kind of prevent silkworm morbidity sericulture method, reduce 5 age silkworms occur nuclear polyhedrosis,
The communicable diseases such as mind-intesting cytoplasmic polyhedrosis, white muscardine improve the security bred silkworms and silk cocoon production so as to cause the yield reduction of silk cocoon
The stability of amount.
To reach above-mentioned purpose, the technical solution adopted by the present invention is:A kind of sericulture method for preventing silkworm to fall ill, it is wrapped
Include the mixture for adding food urea, DHPG, posaconazole, siskin isoflavonoid to 5 age silkworms.
Further, urea, DHPG, posaconazole, the weight ratio of siskin isoflavonoid are 950 in the mixture:
30:10:10。
Further, because silkworm is concentrated, raising, individual are small, it is big to raise quantity, and single head silkworm is difficult in production and is administered respectively,
Therefore the present invention using by urea, DHPG, posaconazole, siskin isoflavonoid 1.5~2.0 grams of mixture with 200~
After the dissolving of 250ml water, 2~2.5 kilograms of mulberry leaf, the method that 5 age silkworms are fed after drying are sprayed.
Further, after being mostly occurred in due to breed silkworms upper nuclear polyhedrosis, mind-intesting cytoplasmic polyhedrosis, white muscardine in 5 ages
Phase, and advanced symptoms after infection not substantially, the course of disease it is short, it is not that every silkworm is infected to raise in colony, considers medicine
The factors such as holdup time and drug cost of the thing in silkworm body, the technical solution adopted by the present invention is:Silkworm, 5 ages from 5 ages
2 days, the 4th day, the mulberry leaf that prepare of the 6th day each feeding claim 31 time, feed 8~10 hours every time, and other time is with normal
Mulberry leaf are raised.
Compared with prior art, the present invention has the advantages that:
The present invention by add food urea, DHPG, posaconazole, siskin isoflavonoid mixture reduce silkworm blood
Type pus illness, mind-intesting cytoplasmic polyhedrosis, the generation of white muscardine, improve the security of sericulture and the stability of cocoon yield, and even in
Cocoon yield can also be increased in the case of not infecting.Present is bred silkworms in production, without effectively for blood type purulence
Disease, mind-intesting cytoplasmic polyhedrosis, white muscardine freshen food medicine, it is main or by putting prevention first, anti-overweight the Comprehensive Control Strategy controlled.
Embodiment
For the technique effect for the technology used in the present invention means being expanded on further He reaching, with reference to embodiments to this
Invention elaborates:
The sericulture method of present invention prevention silkworm morbidity, it comprises the following steps:
1. by urea, DHPG, posaconazole, siskin isoflavonoid using weight ratio as 950:30:10:10 ratio is carried out
Mixing;
2. after said mixture is dissolved with every 1.5~2.0 grams of mixtures with 200~250ml water, spray 2~2.5 kilograms
Mulberry leaf on, dry;
3. by above-mentioned mulberry leaf, silkworm, the 2nd day 5 ages, the 4th day, the 6th day respectively feed once from 5 ages, every time feeding feeding every time
8~10 hours, other time was raised with normal mulberry leaf.
Embodiment one:The infection of nuclear polyhedrosis is reduced, cocoon yield is improved
1st, urea, DHPG, posaconazole, 1.5 grams of the mixture of siskin isoflavonoid are dissolved with 250ml water, sprinkling 2.5
Kilogram mulberry leaf, while spraying mulberry leaf with 250ml clear water, feed silkworm (kind from 5 ages respectively after drying:Bright moon × cyanines pine) 8 hours,
The former is medicine group, and the latter is control group;
2nd, mulberry leaf piece impregnates every ml containing 10 respectively8With 107Silkworm polyhedrosis suspension, after slightly drying, feed respectively
The medicine group of step 1, control group silkworm 6 hours, after use the feeding of normal mulberry leaf instead;
3rd, 5 ages the 2nd day, the medicine group of step 2, control group silkworm distinguish the herbal sprinkling mulberry leaf and clear water of feeding step 1
Spray mulberry leaf 8 hours, after use normal mulberry leaf feeding instead;
4th, 5 ages the 4th day, the medicine group of step 3, control group silkworm distinguish the herbal sprinkling mulberry leaf and clear water of feeding step 1
Spray mulberry leaf 8 hours, after use normal mulberry leaf feeding instead;
5th, 5 ages the 6th day, the medicine group of step 4, control group silkworm distinguish the herbal sprinkling mulberry leaf and clear water of feeding step 1
Spray mulberry leaf 8 hours, after use normal mulberry leaf instead and raise supreme cluster, and count the incidence and the amount of cocoon production of the nuclear polyhedrosis of each group
(table 1).The incidence of disease of medicine group nuclear polyhedrosis is significantly lower than control group, and the cocoon yield of medicine group is apparently higher than control group.
Table 1
Embodiment two, the infection for reducing mind-intesting cytoplasmic polyhedrosis, improve sericulture security
1st, urea, DHPG, posaconazole, 2.0 grams of the mixture of siskin isoflavonoid are dissolved with 200ml water, sprinkling 2,000
Gram mulberry leaf, while spraying mulberry leaf with 250ml clear water, feed silkworm (kind from 5 ages respectively after drying:Bright moon × cyanines pine) 10 hours,
The former is medicine group, and the latter is control group;
2nd, mulberry leaf piece impregnates every ml containing 10 respectively8With 107The polygonal liquid suspension of silkworm matter type, after slightly drying, feed respectively
The medicine group of step 1, control group silkworm 8 hours, after use the feeding of normal mulberry leaf instead;
3rd, 5 ages the 2nd day, the medicine group of step 2, control group silkworm distinguish the herbal sprinkling mulberry leaf and clear water of feeding step 1
Spray mulberry leaf 10 hours, after use normal mulberry leaf feeding instead;
4th, 5 ages the 4th day, the medicine group of step 3, control group silkworm distinguish the herbal sprinkling mulberry leaf and clear water of feeding step 1
Spray mulberry leaf 10 hours, after use normal mulberry leaf feeding instead;
5th, 5 ages the 6th day, the medicine group of step 4, control group silkworm distinguish the herbal sprinkling mulberry leaf and clear water of feeding step 1
Spray mulberry leaf 10 hours, after use normal mulberry leaf instead and raise supreme cluster, sediments microscope inspection is sentenced according to polyhedral body is whether there is in midgut tissue
Determine whether silkworm infects, and count the infection rate (table 1) of the mind-intesting cytoplasmic polyhedrosis of each group, the infection rate of display medicine group is significantly lower than
Control group.
Table 2
Embodiment three, reduction muscardine infection, improve sericulture security
1st, silkworm (kind from 5 ages:Bright moon × cyanines pine) often ml contains 10 for body spray10The suspension of conidia of beauveria bassiana;
2nd, the silkworm of step 1 is divided into medicine group and control group;
3rd, medicine group silkworm:By urea, DHPG, posaconazole, siskin isoflavonoid 1.75 grams of mixture 225ml
Water dissolves, and sprays 2 kilograms of mulberry leaf, is fed 8 hours after drying;Control group silkworm:225ml clear water sprays 2 kilograms of mulberry leaf, after drying
Feeding 8 hours.Raised afterwards with normal mulberry leaf;
4th, the 2nd day 5 ages, the 4th day, the 6th day, the medicine group of step 3, control group silkworm feed respectively herbal sprinkling mulberry leaf and
Clear water sprinkling mulberry leaf 8 hours, other time is fed up to upper cluster with normal mulberry leaf, and counts the incidence of the white muscardine of each group
(table 3).The incidence of medicine group white muscardine is significantly lower than control group.
Table 3
Example IV improves cocoon yield
1st, medicine group silkworm:It is normal to raise to the loose silkworm 100 of the bright moon in 5 ages × cyanines, urea, DHPG, Bo Shakang
Azoles, 2.0 grams of the mixture of siskin isoflavonoid are dissolved with 250ml water, are sprayed 2.5 kilograms of mulberry leaf, silkworm are fed after drying, from 5 ages
Silkworm, the 2nd day, the 4th day, respectively feeding 1 time in the 6th day, feed 8 hours, other time is raised up to upper cluster with normal mulberry leaf every time.
2nd, control group silkworm:It is normal to raise to the loose silkworm 100 of the bright moon in 5 ages × cyanines, spray 2.5 thousand by 250ml clear water
Gram mulberry leaf, feed silkworm after drying, silkworm from 5 ages, the 2nd day, the 4th day, each feeding in the 6th day 1 time, every time feeding 8 hours, Qi Tashi
Between raised with normal mulberry leaf until upper cluster.
3rd, 5 age developmental durations, cocoon yield, cocoon weight, cocoon shell weight, the Cocoon layer ratio (table 4), the receipts of medicine group of each group are counted
Cocoon amount, cocoon weight, cocoon shell weight are higher than control group, are more or less the same between 5 ages process, Cocoon layer ratio medicine group and control group.
Table 4
Embodiment five improves cocoon yield, reduces dead cage rate
1st, medicine group silkworm:Bright moon × cyanines 4 kinds of loose silkworm are normal to raise to 5 ages:Urea, DHPG, Bo Shakang
Azoles, the mixture of siskin isoflavonoid are dissolved by 1.5 grams with 250ml water, spray 2.5 kilograms of mulberry leaf, silkworm, 5 ages are fed after drying
Silkworm, the 2nd day, the 4th day, respectively feeding 1 time in the 6th day are played, every time feeding 8 hours, other time is raised up to upper with normal mulberry leaf
Cluster;
2nd, control group silkworm:Bright moon × cyanines 4 kinds of loose silkworm are normal to raise to 5 ages:2.5 kilograms are sprayed by 250ml clear water
Mulberry leaf, feed silkworm after drying, silkworm, the 2nd day, the 4th day, respectively feeding 1 time in the 6th day from 5 ages, every time feeding 8 hours, other time
Raised with normal mulberry leaf until upper cluster.
3rd, the cocoon yield of each group, and the dead cage rate of sample investigation are counted.Kind of 45.3 kilograms of a cocoon yield for medicine group, extremely
Cage rate is 2.43%;Kind of 41.4 kilograms of a cocoon yield for control group, dead cage rate is 5.74%.Kind of a cocoon yield for medicine group
Higher than control group by 9.42%, dead cage rate is significantly lower than control group.
Described above has fully disclosed the embodiment of the present invention.It is pointed out that being familiar with the field
Scope of any change that technical staff is done to the embodiment of the present invention all without departing from claims of the present invention.
Correspondingly, the scope of claim of the invention is also not limited only to previous embodiment.
Claims (4)
1. a kind of sericulture method for preventing silkworm to fall ill, it is characterised in that:It include to 5 age silkworms add food urea, DHPG,
The mixture of posaconazole, siskin isoflavonoid.
2. the sericulture method of prevention silkworm morbidity according to claim 1, it is characterised in that:Urea in the mixture,
DHPG, posaconazole, the weight ratio of siskin isoflavonoid are 950:30:10:10.
3. the sericulture method of prevention silkworm morbidity according to claim 1, it is characterised in that:It is also included urea, third
After oxygen guanosine, posaconazole, 1.5~2.0 grams of the mixture of siskin isoflavonoid are dissolved with 200~250ml water, sprinkling 2~2.5 thousand
Gram mulberry leaf, feed 5 age silkworms after drying.
4. the sericulture method of prevention silkworm morbidity according to claim 3, it is characterised in that:Silkworm from 5 ages, the 2nd day 5 ages,
4th day, the mulberry leaf that prepare of the 6th day each feeding claim 31 time, feed 8~10 hours, other time uses normal mulberry leaf every time
Raise.
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