CN105123766B - Entomopathogenic nematode HbSD, its insecticide and preparation method and application - Google Patents
Entomopathogenic nematode HbSD, its insecticide and preparation method and application Download PDFInfo
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- CN105123766B CN105123766B CN201510653569.2A CN201510653569A CN105123766B CN 105123766 B CN105123766 B CN 105123766B CN 201510653569 A CN201510653569 A CN 201510653569A CN 105123766 B CN105123766 B CN 105123766B
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Abstract
The invention discloses entomopathogenic nematode HbSD, its insecticide and preparation method and application, wherein the preparation method of suspension preparation includes HbSD infecting last age Yellow meal worm larva, obtain the dead worm that color is changed into rufous within 6th day, continue culture to the 10th day, HbSD is climbed into water out of dead worm corpse, up to suspension；Triton X 100 are added toward suspension, and entomopathogenic nematode HbSD suspension preparations are made；The preparation method of worm corpse agent includes entomopathogenic nematode HbSD infecting last age Yellow meal worm larva, obtains the dead worm that color is changed into rufous within the 6th day, continues culture and obtained worm corpse agent to the 9th day.Suspension preparation and worm corpse agent prepared by the present invention has the advantages that effect stability, production is simple, of low cost, pollution-free, it is high to kill pest activity.
Description
Technical field
The present invention relates to biological insecticides, more particularly to entomopathogenic nematode HbSD, its insecticide and preparation method and should
With.
Background technology
Entomopathogenic nematode is a kind of entomogenous nematode for carrying symbiotic bacteria in vivo, such nematode is as in the world
New biological insecticides, it is extensive with host range；To vertebrate, plant and other non-target organisms safety, it is beneficial to
Environmental protection；It is easy to carry out live body or in vitro mass propgation, it is easy to use；Host can be actively found, is quickly killed off the insect pests；In ring
It can be recycled in border, can be with a variety of chemistry and mixed biological insecticides the advantages that, in prevention a variety of soil pests and hidden evil
It played an important role in terms of worm.
Entomopathogenic nematode is under the jurisdiction of animal kingdom's Nemathelminthes (Nematoda), phasmid (Secernentea), small bar
Mesh (Rhabditida), up to now, it is known that the nematode for parasitizing insect be about 40 Duo Ge sections, wherein using more desinsection
Nematode includes Steinernema Carpocapsae (Steinernema carpocapsae, Sc) and Heterorhabditis bacteriophora-NJ (Heterorhabditis
Bacteriophora, Hb).Nematode and the bacterium of its parachorium act synergistically to kill insect.Nematode for desinsection is recognized
To be the biological insecticides to environment and mankind close friend.Desinsection nematode is widely used to soil dwelling property pest at present, as chafer,
The prevention of the pest such as wireworm, cutworm, mole cricket, root maggot, leek maggot.Application process includes nematode spray suspension liquid and nematode sense
Worm corpse after dye, but due to its spray suspension liquid need to add 4-5 kinds auxiliary agent and protective agent (such as anti-evaporating agent, water-retaining agent, adhesive,
Uv-protector), cause its production cost high, and the shortcomings such as prepared insecticide insecticide efficiency is low influence its application.
The content of the invention
A kind of entomopathogenic nematode HbSD provided by the invention, improves insecticide efficiency.
On the other hand, suspension preparation provided by the invention, which solves biological insecticides production process, need to increase a variety of auxiliary agents
Cause the problems such as of high cost with protective agent.
The entomopathogenic nematode HbSD of the present invention, by the way that young age yellow meal worm is placed in nematode, makes last age behind some days
Yellow meal worm is changed into the dead worm of rufous, further filters out nematode HbSD.
The preparation method of entomopathogenic nematode HbSD suspension preparations, including HbSD is infected into last age Yellow meal worm larva, the 6th
It obtains color and is changed into the dead worm of rufous, continues culture on wet filter paper to the 10th flying worm HbSD infective stage larvas from dead worm corpse
Climb out of in vivo, into water, up to suspension, be maintained at 10-12 DEG C；Triton X-100 are added during use toward suspension to be made
HbSD suspension preparations.
Entomopathogenic nematode HbSD suspension preparations, including entomopathogenic nematode HbSD and Triton X-100.
Entomopathogenic nematode HbSD suspension preparations, by entomopathogenic nematode that concentration is 900-1000 bars/mL during use
The Triton X-100 that HbSD stostes are made into 27-30 bars/mL entomopathogenic nematodes HbSD and volume accounting is 0.05%.
The preparation method of entomopathogenic nematode HbSD worm corpse agent, infects last age yellow meal worm children using entomopathogenic nematode HbSD
Worm, obtains the dead worm that color is changed into rufous on the 6th day, continues culture and obtained worm corpse to the 9th day.
Entomopathogenic nematode HbSD suspension preparations are used to prevent insect, and insect is last age greater wax moth larva, 3 age Sugarcane Yellows
Snout moth's larva larva, last age Yellow meal worm larva, Brontispa longissima larva or last age sack moth larvae.
The agent of entomopathogenic nematode HbSD worm corpse is used to prevent insect, and insect is last age greater wax moth larva, 3 age Sugarcane Yellow snout moth's larvas children
Worm, last age Yellow meal worm larva, last age Brontispa longissima larva or last age sack moth larvae..
The present invention obtains entomopathogenic nematode HbSD by screening, and finds that it has good effect to pest control
Fruit.
Further, nematode suspension preparation of the invention and worm corpse agent.Both preparations are as insecticide, preparation method
And methods for using them is simpler.Compared with prior art, the preparation and application of nematode killer is simplified.
The entomopathogenic nematode HbSD suspension preparations and worm corpse agent of the present invention be respectively provided with effect stability, production it is simple, into
The advantages that this is cheap, pollution-free, bactericidal activity is high.
Brief description of the drawings
Fig. 1 is pesticidal experimental result picture of 4 kinds of mass production nematodes to last age greater wax moth larva；
Fig. 2 is entomopathogenic nematode HbSD infective stage larvas respectively to 3 age Sugarcane Yellow snout moth's larva larvas and last age Yellow meal worm larva
Pesticidal experimental result picture；
Fig. 3 is entomopathogenic nematode HbSD worm corpse agent and entomopathogenic nematode HbSD suspension preparations respectively to Sugarcane Yellow snout moth's larva
Prevention effect figure；
Fig. 4 is that garden sprays the experimental result that entomopathogenic nematode HbSD suspension preparations prevent cinnamomum camphora Clania variegata Snellen pest
Figure.
Embodiment
The present invention is described in further detail below by embodiment combination attached drawing.
The entomopathogenic nematode HbSD (for heterorhabditis indica referred to as, SD is numbering to Hb) of the present invention is a kind of different small bar line
Worm, can be by the side's of trapping acquisition in the wild, specific method：Last age Yellow meal worm larva is placed in nylon wire, is embedded to Field Soil
In, harvest color is changed into the yellow meal worm of rufous within the 6th day, parasitizes Insect Pathogenic line of the nematode in yellow meal worm for the present invention
Worm HbSD.HbSD has good fecundity, can also obtain its offspring by artificial mass propgation.
Entomopathogenic nematode HbSD infective stage larvas (IJs) are the insect larvae after infected insect pathogenic nematode HbSD.
Embodiment one：The preparation method of entomopathogenic nematode HbSD suspension preparations
The first entomopathogenic nematode HbSD insecticides are suspension preparation, and its preparation method includes：
The preparation method of entomopathogenic nematode HbSD suspension preparations：One layer of filter paper is put in plastic box bottom, selects growth
The last age Yellow meal worm larva 100 of health is placed on filter paper, and drawing the HbSD nematodes that concentration is 1000/ml with liquid-transfering gun suspends
Drop is added on last age yellow meal worm, is covered, and polypide color change situation is checked after room temperature is placed 4-5 days：6th day, normal sense
The yellow meal worm color of dye entomopathogenic nematode is changed into rufous, continues to cultivate after discarding off-colour yellow meal worm, the 10th day
Harvest has infected the yellow meal worm of entomopathogenic nematode HbSD.By " white trapping " method, that is, utilize HbSD infective stage larvas (IJs)
Hydrotaxis collect nematode, nematode crawled into water out of insect bodies, and first day harvest discarded, by the infection period of the 2-3 days
Larva suspension checks survival rate under the microscope, and the survival rate of nematode is greater than 95%；Then 10-15 DEG C of refrigerator is deposited in supply
Experiment connects use in next step.
The nematode suspension concentration of storage is diluted to 900-1000 bars nematode/milliliter using preceding, be diluted to 27-30 bars/
The entomopathogenic nematode HbSD of mL, Triton X-100 are added in the ratio of liquor capacity 0.05%.
Triton X-100 are Triton X-100, are a kind of nonionic surface active agent, have and uniformly divide
Dissipate and sticky effect.
The present embodiment selects single auxiliary agents of the Triton X-100 as nematode HbSD suspension, and cost is low, insecticide efficiency
Ability that is high and not influencing nematode searching parasitism.
Embodiment two：The preparation of entomopathogenic nematode HbSD worm corpse agent
Second of entomopathogenic nematode HbSD insecticide is worm corpse agent, and its preparation method includes：
The preparation method of entomopathogenic nematode HbSD worm corpse agent：It will be infected with the entomopathogenic nematode HbSD of harvest within 1st day
The last age Yellow meal worm larva of phase larva infection.Selection color is changed into the yellow meal worm of rufous within 6th day, continues to cultivate, the 9th day harvest worm
Corpse, is placed in 50-100mL centrifuge tubes, is saved backup under the conditions of 10-14 DEG C.
The survival rate of entomopathogenic nematode HbSD is greater than 95%, and the agent of worm corpse is the metainfective last age yellow meal worm childrens of HbSD
Worm, each worm corpse at least contain 5000 entomopathogenic nematode HbSD.
Two kinds of HbSD nematode killers of the present invention：Nematode suspension preparation and worm corpse agent.Preparation method and application method
It is simpler.Compared with prior art, the preparation and application of nematode killer is simplified.
Embodiment three：The insecticidal properties experiment of entomopathogenic nematode HbSD
For trying nematode strain：4 kinds of Heterorhabditis bacteriophora-NJ category nematodes HbSD, HbI, HbII and HbIII.
For trying insect：Last age greater wax moth larva
Test method：Indoor bioassay method, that is, measure 4 kinds of mass production nematode suspension to last age greater wax moth
The virulence of larva, the death rate is counted after 6 days.Control group does not add nematode.
As a result with analysis：4 kinds of mass production nematodes are to the pesticidal as shown in Figure 1, four of last age greater wax moth larva
Kind is for trying in nematode strain (HbSD, HbI, HbII, HbIII), the insecticidal power highest of entomopathogenic nematode HbSD of the invention,
Fecundity is strong.Selection HbSD is used for further toxicity test.
Example IV：
For trying nematode strain：Entomopathogenic nematode HbSD infective stage larvas
For trying insect：Sugarcane Yellow snout moth's larva (Tetramoera schistaceana) 3 instar larvaes and yellow meal worm linal-instar larvae
Indoor bioassay method, respectively by 3 age sugarcane borer larvas or yellow meal worm linal-instar larvae and entomopathogenic nematode
The number ratio of HbSD infective stage larvas is 1:100 are put into culture dish, each to add 200ul water treatments, each processing 36
3 instar larvae of sugarcane borer or yellow meal worm linal-instar larvae, are placed at room temperature, and control experiment is infected with being not added with entomopathogenic nematode HbSD
Phase larva, only adds tap water, the death rate is counted after 6 days.
As shown in Fig. 2, test result indicates that entomopathogenic nematode HbSD is to 3 instar larvae of Sugarcane Yellow snout moth's larva and last age yellow meal worm children
Worm is respectively provided with significant virulence, the LC of 3 instar larvae of Sugarcane Yellow snout moth's larva50It is worth for 9.00/head, the LC of yellow meal worm linal-instar larvae50It is worth and is
16.80 bar/head.
Embodiment five：
Material to be tested：Entomopathogenic nematode HbSD infective stage larvas；
For trying insect：Greater wax moth (Galleria mellonella.) linal-instar larvae, Brontispa longissima (Brontispa
Longissim) linal-instar larvae, Clania variegata Snellen (Cryptothelea variegate) linal-instar larvae.Manually feed cultivates big wax
Snout moth's larva, with fresh coconut leaf feeding Brontispa longissima；From leaf of Cortex cinnamomi camphorae feeding sack moth larvae, 25 DEG C of artificial climate cage breedings are placed in
Grow.
The ratio of all insect infection entomopathogenic nematode infective stage larvas is the same, by insect and pathogenic nematode infection period children
Worm adds the culture dish bottom that bottom is covered with the graceful filter paper of shellfish, for trying insect Clania variegata Snellen 200, Brontispa longissima 300, greater wax moth
400.Each processing is repeated 4 times, and the death rate is recorded after 6 days.
HbSD infective stage larvas to Brontispa longissima linal-instar larvae, Clania variegata Snellen linal-instar larvae, greater wax moth linal-instar larvae virulence
As shown in table 1, with 8.6-11.7 HbSD heads/mL dosage, it can kill for 50% Brontispa longissima, Clania variegata Snellen, greater wax moth last age
Larva.
Table 1
Embodiment six：
Material to be tested：The entomiasis that embodiment 1 prepares entomopathogenic nematode HbSD suspension preparations and prepared by embodiment two
Former nematode HbSD worms corpse agent
For trying field：There is the sugarcane field of yellow snout moth's larva harm
Using the spray method of suspension preparation, suspension spray amount is 1.02 × 104Bar/m2, spray once within one week, even
It is three weeks continuous, each plot area 4m2, the buffer strip that 5 meter of minizone, each handles 6 replicated plots.
The infective stage larva in the worm corpse of embodiment two is counted under anatomical lens, it is interior containing about 7.71 × 10 per cephalont corpse4Elder brother
Worm pathogenic nematode HbSD infective stage larvas, worm corpse landfill lower 5-8 centimetres of depths of soil around sugarcane plant, are spaced 85 centimetres one
Hole, 2 cephalont corpse, 6 repetitions are filled in each hole.To be not added with worm corpse as control group, investigation records result is distinguished after 30 days.Each
Heatable adobe sleeping platform (1 meter of interval) buries 2 HbSD nematode infections phase worm corpse, it is possible to reduce landfill number, improves insecticide efficiency, reach prevention
The purpose of pest.
As Fig. 3's test result indicates that, HbSD worm corpse agent (the yellow meal worm corpse of HbSD nematode infections) and HbSD suspension
Prevention of the preparation to Sugarcane Yellow snout moth's larva.After handling 30d using HbSD suspension preparations, the relative survival rate of Sugarcane Yellow snout moth's larva larva compares
Less than 30% is reduced to according to group.After worm corpse agent processing 30d, the relative survival rate of Sugarcane Yellow snout moth's larva larva is reduced to 61% than control group
Below.Preferable prevention effect is respectively provided with to Sugarcane Yellow snout moth's larva using HbSD suspension preparations and HbSD worm corpse agent.
Embodiment seven：
Material to be tested：Entomopathogenic nematode HbSD suspension preparations prepared by embodiment one；
Test field：The more serious area of cinnamomum camphora tree Clania variegata Snellen morbidity
The more serious cinnamomum camphora tree that falls ill is carried out using entomopathogenic nematode HbSD suspension preparations prepared by embodiment one
Blade back sprays；Spray once within every 15 days, investigation prevention result after totally 3 times, 3 months.
Garden sprays HbSD suspension preparations prevention cinnamomum camphora Clania variegata Snellen pest, and the results are shown in Figure 4, and garden sprays HbSD and hangs
Supernatant liquid preparation can effectively prevent the outburst of cinnamomum camphora Clania variegata Snellen pest for 3 times.
Above content is to combine specific embodiment further description made for the present invention, it is impossible to assert this hair
Bright specific implementation is confined to these explanations.For general technical staff of the technical field of the invention, do not taking off
On the premise of from present inventive concept, some simple deduction or replace can also be made.
Claims (3)
- A kind of 1. entomopathogenic nematode HbSD suspension preparations, it is characterised in that：Including entomopathogenic nematode HbSD and Triton X-100。
- 2. entomopathogenic nematode HbSD suspension preparations according to claim 1, it is characterised in that：It is 27- including concentration The entomopathogenic nematode HbSD and volume accounting of 30/mL is 0.05% Triton X-100.
- 3. the entomopathogenic nematode HbSD suspension preparations described in claim 1 or 2 are used for the application for preventing insect, the insect For last age greater wax moth larva, 3 age Sugarcane Yellow snout moth's larva larvas, last age Yellow meal worm larva, last age Brontispa longissima larva or last age Clania variegata Snellen Larva.
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| WO2020193969A1 (en) * | 2019-03-25 | 2020-10-01 | Bionema Ltd | Pest control kit and method |
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| CN110199743A (en) * | 2019-07-12 | 2019-09-06 | 浙江绿神天敌生物技术有限公司 | A kind of control method of Spodopterafrugiperda |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1180375A (en) * | 1995-02-02 | 1998-04-29 | 洛特格斯新泽西州立大学 | Heterorhabditis bacteriophora insecticide compositions and related processes |
| CN103053620A (en) * | 2011-10-20 | 2013-04-24 | 南开大学 | Entomopathogenic nematodes insect corpse agent |
| CN103688907A (en) * | 2013-12-10 | 2014-04-02 | 甘肃农业大学 | Entomopathogenic nematode rejuvenation method |
| CN104886153A (en) * | 2015-05-21 | 2015-09-09 | 云南省烟草公司玉溪市公司 | Method for preventing tobacco root-knot nematode by using entomopathogenic nematode suspending liquid |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US20050244387A1 (en) * | 2004-04-28 | 2005-11-03 | Parwinder Grewal | Entomopathogenic nematodes and methods of their use |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1180375A (en) * | 1995-02-02 | 1998-04-29 | 洛特格斯新泽西州立大学 | Heterorhabditis bacteriophora insecticide compositions and related processes |
| CN103053620A (en) * | 2011-10-20 | 2013-04-24 | 南开大学 | Entomopathogenic nematodes insect corpse agent |
| CN103688907A (en) * | 2013-12-10 | 2014-04-02 | 甘肃农业大学 | Entomopathogenic nematode rejuvenation method |
| CN104886153A (en) * | 2015-05-21 | 2015-09-09 | 云南省烟草公司玉溪市公司 | Method for preventing tobacco root-knot nematode by using entomopathogenic nematode suspending liquid |
Non-Patent Citations (1)
| Title |
|---|
| 新疆嗜菌异小杆线虫及其共生细菌的初步鉴定研究;詹发强等;《新疆农业科学》;20150311;244-251 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020193969A1 (en) * | 2019-03-25 | 2020-10-01 | Bionema Ltd | Pest control kit and method |
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