CN1180375A - Heterorhabditis bacteriophora insecticide compositions and related processes - Google Patents
Heterorhabditis bacteriophora insecticide compositions and related processes Download PDFInfo
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- CN1180375A CN1180375A CN96192982A CN96192982A CN1180375A CN 1180375 A CN1180375 A CN 1180375A CN 96192982 A CN96192982 A CN 96192982A CN 96192982 A CN96192982 A CN 96192982A CN 1180375 A CN1180375 A CN 1180375A
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Abstract
This invention provides an insecticidal composition comprising an effective amount of a juvenile infective-stage of insecticidal entomopathogenic Heterorhabditis bacteriophora hybrid strain Hb (NJ), or mutants or variants thereof and a biocompatible carrier therefor. The insecticidal compositions provided have improved insecticidal activity compared to known insecticidal compositions containing a known H. bacteriophora nematode HBHP88. Also disclosed is a process for reducing the population of soil-inhabiting insects in soil by applying to a soil locus infected with such insects the above-described insecticidal composition. Additionally, the Hb (NJ) hybrid nematode strain is provided.
Description
The field of the invention
The present invention relates to contain the new insecticides that contains infective stage abnormal shape shaft-like (heterorhabditid) hybridization nematode with enhanced insecticidal effect; New nematode with this feature; And by practising the method that occupies the insect insect population to reduce soil infecting this insect-killing composition of using effective effect amount in the soil place.
Background of the present invention
The insecticides that contains biotic pesticide or entomopathogenicity nematode composition has been opened up one and noticeable has been practised the field that occupies insect with the anti-soil of biocontrol agent.They have many important advantages than commercially available chemical insecticide.The superiority of nematode sterilant comprises high toxicity, the host range of wide spectrum, and security, government registries exempts from taxation and is easy to lot production.
The nematode of abnormal shape rhabditis-form family has constituted the distinctive symbiosis contact with the Photorhabdus bacterium.They have pathogenicity bo to the multigroup insect.Special-shaped rhabdos is hermaphroditic.Usually, the communicable young normally penetrates the haemocoele of its host insect such as white grub by intestines or valve.Thereby special-shaped rhabdos has a back of the body tooth is convenient to penetrate other zone (as intersegmental membrane).The bacterium of nematode Digestive tract is released in the host, and this host generally can die from septicemia in 48 hours.This nematode is fed in host's corpse, grows, ripe and breeding, and after generation, produce infective larva how again usually, and sprout wings, seek new host insect again.
Be desirable to provide and contain those and have the insecticides that improved anti-soil is practised the sterilant nematode that occupies the insect effect; New nematode with this improved effect; Use this improved sterilant nematode to practise the method for harmful insect that occupies with control soil; And technology and the equipment of producing this improved sterilant nematode.
General introduction of the present invention
Thereby provided by the present invention is improving one's methods of new insecticides and the harmful insect of controlling soil habit residence by the new insecticides of using the entomopathogenicity nematode in the infective stage of the present invention of containing effective dose in existing insect pest place.What insecticides of the present invention comprised effective dose has Heterorhabditis bacteriophora hybrid strain HB (NJ) and effective varient thereof, the parasiticidal entomopathogenicity nematode in the infective stage of the juvenile form of the parasiticidal entomopathogenicity nematode in the infective stage of the juvenile form of the sterilant characteristic of mutant or hybrid and the sterilant characteristic of survivor hybrid for this reason and a biological fitness carrier for this reason.The new entomopathogenicity nematode that also has in the insect-killing composition of the present invention provided by the present invention, it practises occupying at control soil has high efficiency aspect the insect, comprises the pathogenicity bo and the enhanced fecundity that the various insects host there are raising.Detailed description of the present invention
This Heterorhabditis bacteriophora HB (NJ) hybrid strain is to obtain by hybridizing a big serial wild-type strain isolated from four different H.bacteriophora of perching gained the collected soil sampling.This perches institute is the meadow, the long meadow that scattered tree is arranged, and infested with weeds open country and the long infested with weeds open country that scattered tree is arranged, this perches the many bleeding points of representative.
The enhanced characteristic of this hybrid strain comprises the fecundity to various insects host enhanced pathogenicity bo or infectivity and raising.
By following test determination infectivity.Used infectivity stdn test is the top that 50 communicable larvas is positioned over sand post (5cm diameter * 5cm height), and there is a kind of target insect (wax moth, Galleria mellonella) the sand column bottom.This test generally is to carry out under 25 ℃, and contains the moisture of 10% weight percent in the sand.Allow this nematode to have 24 hours to seek the target insect, constantly take out this insect, rinsing and under 25 ℃, cultivating again 24 hours in distilled water so that this nematode grows in the host if having time at that.Then, dissect host and count the number that those successfully are settled in the nematode among the host.Repeat in two groups of tests of 20 times at every group, the average infection rate of HB (NJ) has 10.45 and 11.1 nematodes (standard deviation (SE) is respectively 1.053 and 1.095) for each host, all infected and death in experimentation of hosts.In comparable test, the result of this HB (NJ) strain performance is more much better than two kinds of known H.bacteriophora strains (HBNC and HBHP88).They only kill 50% and 70% host respectively, and average infection rate is respectively that each host has only 0.08 and 1.20 nematode.This HB (NJ) strain has also shown its similar advantage to the infectious rate of other insect.
Fecundity is by following test determination.The test of the fecundity of standard is at 25 ℃ of quantitative numbers of those new communicable larvas of generation from the single host (Galleria mellonella) of the standard weights (0.24-0.26 gram) of the conventional inoculation of communicable larva (n=20) down.About 30 repeated experiments with HB (NJ), the average fecundity level that obtains is 383,046.7 (standard deviation (SE) is 15212.9).The fecundity that HB (NJ) is described is superior.
Heterorhabditis bacteriophora hybrid strain HB provided by the present invention (NJ) has preserving number ATCC_.
This insecticides can be with aqueous composite spray form; To use Protanal TXF 200 or its form to exist.
Being used for fixing nematode or their carrier of part drying can slow down the metabolic of them and improve its tolerance to extreme temperature.Though make that at room temperature the effect of storage is generally relatively poor enduringly because the viability of nematode descends rapidly, but can buy the matrix of surviving in humidity such as foam, the nematode on vermiculite and the peat.Heterorhabditis composition of the present invention is suitable for being stored in 10 ℃-15 ℃ times and this organism should remain in the environment of aerobic.
Can use to comprise the small pressure atomizer, the spraying gas blower, electrostatic atomiser, the fan atomizer, and the agrochemistry equipment commonly used of aerial spraying machine is used nematode.The entomopathogenicity nematode can tolerate 300 pounds/inch
2Pressure and can be with little spout type atomizer (as " 01 " shower nozzle) sprinkling to 50 microns of all conventional opening diameters.Yet the dispensing apparatus of some types produces a large amount of heat, has secondary face to ring in case the temperature in this atomizer pipeline rises to more than 32 ℃ to this nematode.Its seldom takes place and almost is the problem that takes place under the situation empty and that almost do not absorb heat water at this atomizer case just usually fortunately.
Normally used field concentration is above 2,500,000,000 nematode/hectares (1,000,000,000/mu) thereby to guarantee the having nematode of enough insect populations to contact the generation control action kou with the target insect.Need high density to overcome the negative influence of abiotic and lived edatope.Also need high nematode concentration to tackle the insect that some only stopped several days before piercing root in soil, as Caulis et Folium Brassicae capitatae maggot (D.radicum), and tackle those owing to volume the little and mobile insect that is difficult for the nematode infection flexibly.For example, need the nematode of high density to tackle the mole cricket (Scapteriscus spp.) of early stage mezzanine level, root maggot (Delia spp.) and Zea mays root worm (Diabroticaspp.).
About being applicable to that discussing widely of prescription that insect-killing composition of the present invention is provided and application and relevant technology of the present invention sees the collection of thesis that Randy doctor Gaugler and Harry doctor K.kaya edit, " application of entomopathogenic nematode in biological and ecological methods to prevent plant disease, pests, and erosion ", the 9th chapter, " Ramon Georgis prescription and utilisation technology ", (the Boca Raton of CRC press, Ann Arbor, Boston), 1990.
The Galleria mellonella identification test of insecticidal activity of nematode of being used to test and assess carries out as follows, No. 1 filter paper of Whatman is placed the culture dish of 35mm.Again Galleria mellonella host is placed on the filter paper.With 100 childhood nematode be suspended in 500 microliters of water.By the method for drenching these several places of filter paper aqeous suspension is placed on the paper.If this Galleria mellonella reddens in test then show that Galleria mellonella host is dead latter stage.
In the Galleria mellonella bait method that is used for separating nematode, the Galleria mellonella that has infected Heterorhabditisbacteriophora usually can be dead and be become redness.The Galleria mellonella of the bacteriophora of the soil-like of being got usually can be dead and be become redness.The diameter of the soil-like of being got is that 2 centimetres and length are 15 centimetres.For Heterorhabditisbacteriophora hybrid strain HB of the present invention (NJ) is provided, 150 Heterorhabditis bacteriophora strain isolateds have approximately been used.
Be used to provide the breeding by crossing of this HB (NJ) bacterial strain to comprise conventional step.
What provided is conspicuous to change of the present invention and those those of skill in the art of revising for this area, and the change within spirit of the present invention and to revise also be within the scope of the present invention.
Claims (6)
1. one kind is used to reduce one or more soil and practises the insect-killing composition that occupies the insect insect population, and it comprises the juvenile form parasiticidal entomopathogenicity nematode of infective stage and the biological fitness carrier for this reason of the insecticidal properties of a kind of Heterorhabditis of having bacteriophora hybrid strain Hb (NJ) of effective dose or its effective varient or mutant.
2. the composition of claim 1, nematode wherein is Heterorhabditis bacteriophora hybrid strain Hb (NJ).
3. one kind is used to reduce the white grub in soil habit residence or the method for other insect insect population, and its soil place that is included in this insect infestation uses the insect-killing composition of a kind of parasiticidal entomopathogenicity nematode that is in infective stage of the insecticidal properties with Heterorhabditis bacteriophora hybrid strain Hb (NJ) or its varient or mutant that contains effective dosage.
4. the method for claim 3, nematode wherein is Heterorhabditisbacteriophora hybrid strain Hb (NJ) nematode in infective stage.
5. the nematode of a Heterorhabditis bacteriophora hybrid strain HB (NJ), it imposes on the soil place of damaged by vermin in juvenile form and can reduce one or more soil and practise the insect insect population that occupies in infective stage.
6. a kind of juvenile form infective stage nematode of claim 5.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96192982A CN1180375A (en) | 1995-02-02 | 1996-01-25 | Heterorhabditis bacteriophora insecticide compositions and related processes |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/382,430 | 1995-02-02 | ||
| CN96192982A CN1180375A (en) | 1995-02-02 | 1996-01-25 | Heterorhabditis bacteriophora insecticide compositions and related processes |
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| CN1180375A true CN1180375A (en) | 1998-04-29 |
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| CN96192982A Pending CN1180375A (en) | 1995-02-02 | 1996-01-25 | Heterorhabditis bacteriophora insecticide compositions and related processes |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105123766A (en) * | 2015-10-10 | 2015-12-09 | 中国热带农业科学院环境与植物保护研究所 | Entomopathogenic nematode HbSD and insecticide thereof, preparation method and application |
-
1996
- 1996-01-25 CN CN96192982A patent/CN1180375A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105123766A (en) * | 2015-10-10 | 2015-12-09 | 中国热带农业科学院环境与植物保护研究所 | Entomopathogenic nematode HbSD and insecticide thereof, preparation method and application |
| CN105123766B (en) * | 2015-10-10 | 2018-05-04 | 中国热带农业科学院环境与植物保护研究所 | Entomopathogenic nematode HbSD, its insecticide and preparation method and application |
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