CN106350490B - 利用无血清培养基从成纤维细胞中获得神经细胞的方法 - Google Patents
利用无血清培养基从成纤维细胞中获得神经细胞的方法 Download PDFInfo
- Publication number
- CN106350490B CN106350490B CN201610952074.4A CN201610952074A CN106350490B CN 106350490 B CN106350490 B CN 106350490B CN 201610952074 A CN201610952074 A CN 201610952074A CN 106350490 B CN106350490 B CN 106350490B
- Authority
- CN
- China
- Prior art keywords
- cell
- nerve cell
- concentration
- final concentration
- serum free
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 210000002569 neuron Anatomy 0.000 title claims abstract description 67
- 238000000034 method Methods 0.000 title claims abstract description 38
- 239000012679 serum free medium Substances 0.000 title claims abstract description 35
- 210000002950 fibroblast Anatomy 0.000 title claims abstract description 31
- 210000004027 cell Anatomy 0.000 claims abstract description 90
- 239000007640 basal medium Substances 0.000 claims abstract description 18
- 210000002966 serum Anatomy 0.000 claims abstract description 14
- 108090000412 Protein-Tyrosine Kinases Proteins 0.000 claims abstract description 12
- 102000004022 Protein-Tyrosine Kinases Human genes 0.000 claims abstract description 12
- 239000003102 growth factor Substances 0.000 claims abstract description 12
- 239000000654 additive Substances 0.000 claims abstract description 11
- 230000000996 additive effect Effects 0.000 claims abstract description 11
- -1 small molecule compound Chemical class 0.000 claims abstract description 11
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 claims description 62
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 claims description 47
- 102000002070 Transferrins Human genes 0.000 claims description 32
- 108010015865 Transferrins Proteins 0.000 claims description 32
- 102000004877 Insulin Human genes 0.000 claims description 31
- 108090001061 Insulin Proteins 0.000 claims description 31
- 229940125396 insulin Drugs 0.000 claims description 31
- 102100024785 Fibroblast growth factor 2 Human genes 0.000 claims description 26
- 108090000379 Fibroblast growth factor 2 Proteins 0.000 claims description 26
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 claims description 24
- 239000001963 growth medium Substances 0.000 claims description 24
- 229960001471 sodium selenite Drugs 0.000 claims description 24
- 235000015921 sodium selenite Nutrition 0.000 claims description 24
- 239000011781 sodium selenite Substances 0.000 claims description 24
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 claims description 13
- 239000000203 mixture Substances 0.000 claims description 11
- 239000002771 cell marker Substances 0.000 claims description 9
- 230000006698 induction Effects 0.000 claims description 9
- 230000010261 cell growth Effects 0.000 claims description 7
- 210000005036 nerve Anatomy 0.000 claims description 7
- 238000010166 immunofluorescence Methods 0.000 claims description 5
- 239000007758 minimum essential medium Substances 0.000 claims description 5
- 238000011534 incubation Methods 0.000 claims description 4
- 101000979001 Homo sapiens Methionine aminopeptidase 2 Proteins 0.000 claims description 3
- 101000969087 Homo sapiens Microtubule-associated protein 2 Proteins 0.000 claims description 3
- 101001092197 Homo sapiens RNA binding protein fox-1 homolog 3 Proteins 0.000 claims description 3
- 102100021118 Microtubule-associated protein 2 Human genes 0.000 claims description 3
- 102100035530 RNA binding protein fox-1 homolog 3 Human genes 0.000 claims description 3
- 239000012980 RPMI-1640 medium Substances 0.000 claims description 3
- 150000003384 small molecules Chemical class 0.000 claims description 3
- UZOVYGYOLBIAJR-UHFFFAOYSA-N 4-isocyanato-4'-methyldiphenylmethane Chemical compound C1=CC(C)=CC=C1CC1=CC=C(N=C=O)C=C1 UZOVYGYOLBIAJR-UHFFFAOYSA-N 0.000 claims description 2
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 claims description 2
- 229930182816 L-glutamine Natural products 0.000 claims description 2
- 102100038126 Tenascin Human genes 0.000 claims description 2
- 108010008125 Tenascin Proteins 0.000 claims description 2
- 230000012010 growth Effects 0.000 claims description 2
- 239000000126 substance Substances 0.000 claims description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 16
- 201000010099 disease Diseases 0.000 abstract description 15
- 239000004017 serum-free culture medium Substances 0.000 abstract description 2
- 230000002103 transcriptional effect Effects 0.000 abstract description 2
- 241000699666 Mus <mouse, genus> Species 0.000 description 30
- 210000001178 neural stem cell Anatomy 0.000 description 14
- 210000000130 stem cell Anatomy 0.000 description 11
- 208000015122 neurodegenerative disease Diseases 0.000 description 8
- 208000024827 Alzheimer disease Diseases 0.000 description 7
- 239000006143 cell culture medium Substances 0.000 description 7
- 230000004770 neurodegeneration Effects 0.000 description 7
- 230000008569 process Effects 0.000 description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- 238000000338 in vitro Methods 0.000 description 6
- 239000003550 marker Substances 0.000 description 6
- 108010082117 matrigel Proteins 0.000 description 6
- 241000699670 Mus sp. Species 0.000 description 5
- 239000006285 cell suspension Substances 0.000 description 5
- 238000005138 cryopreservation Methods 0.000 description 5
- 230000004069 differentiation Effects 0.000 description 5
- 238000010790 dilution Methods 0.000 description 5
- 239000012895 dilution Substances 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- HJCMDXDYPOUFDY-WHFBIAKZSA-N Ala-Gln Chemical compound C[C@H](N)C(=O)N[C@H](C(O)=O)CCC(N)=O HJCMDXDYPOUFDY-WHFBIAKZSA-N 0.000 description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 102000013127 Vimentin Human genes 0.000 description 4
- 108010065472 Vimentin Proteins 0.000 description 4
- 210000001015 abdomen Anatomy 0.000 description 4
- 238000013459 approach Methods 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 235000019441 ethanol Nutrition 0.000 description 4
- 230000036541 health Effects 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 238000004321 preservation Methods 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- 238000012549 training Methods 0.000 description 4
- 210000004291 uterus Anatomy 0.000 description 4
- 210000005048 vimentin Anatomy 0.000 description 4
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
- 239000006147 Glasgow's Minimal Essential Medium Substances 0.000 description 3
- 230000032683 aging Effects 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 230000029087 digestion Effects 0.000 description 3
- 230000009977 dual effect Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 210000000630 fibrocyte Anatomy 0.000 description 3
- 238000007710 freezing Methods 0.000 description 3
- 230000008014 freezing Effects 0.000 description 3
- 210000001161 mammalian embryo Anatomy 0.000 description 3
- 230000001537 neural effect Effects 0.000 description 3
- 230000000149 penetrating effect Effects 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 230000008439 repair process Effects 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 239000011550 stock solution Substances 0.000 description 3
- 238000010257 thawing Methods 0.000 description 3
- OZFAFGSSMRRTDW-UHFFFAOYSA-N (2,4-dichlorophenyl) benzenesulfonate Chemical compound ClC1=CC(Cl)=CC=C1OS(=O)(=O)C1=CC=CC=C1 OZFAFGSSMRRTDW-UHFFFAOYSA-N 0.000 description 2
- FWBHETKCLVMNFS-UHFFFAOYSA-N 4',6-Diamino-2-phenylindol Chemical compound C1=CC(C(=N)N)=CC=C1C1=CC2=CC=C(C(N)=N)C=C2N1 FWBHETKCLVMNFS-UHFFFAOYSA-N 0.000 description 2
- 239000012591 Dulbecco’s Phosphate Buffered Saline Substances 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 208000012902 Nervous system disease Diseases 0.000 description 2
- 108091023040 Transcription factor Proteins 0.000 description 2
- 102000040945 Transcription factor Human genes 0.000 description 2
- 210000004504 adult stem cell Anatomy 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- 238000004043 dyeing Methods 0.000 description 2
- 210000001671 embryonic stem cell Anatomy 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 238000010369 molecular cloning Methods 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 230000010355 oscillation Effects 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 238000012856 packing Methods 0.000 description 2
- 210000001778 pluripotent stem cell Anatomy 0.000 description 2
- 239000002243 precursor Substances 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000001172 regenerating effect Effects 0.000 description 2
- 230000008672 reprogramming Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 210000000717 sertoli cell Anatomy 0.000 description 2
- 229940126586 small molecule drug Drugs 0.000 description 2
- 230000000392 somatic effect Effects 0.000 description 2
- 208000020431 spinal cord injury Diseases 0.000 description 2
- 238000002560 therapeutic procedure Methods 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- 230000007704 transition Effects 0.000 description 2
- 238000002054 transplantation Methods 0.000 description 2
- 230000005740 tumor formation Effects 0.000 description 2
- 210000002700 urine Anatomy 0.000 description 2
- 229940124596 AChE inhibitor Drugs 0.000 description 1
- 101100519158 Arabidopsis thaliana PCR2 gene Proteins 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 208000022306 Cerebral injury Diseases 0.000 description 1
- 206010008190 Cerebrovascular accident Diseases 0.000 description 1
- 206010011878 Deafness Diseases 0.000 description 1
- 208000032612 Glial tumor Diseases 0.000 description 1
- 206010018338 Glioma Diseases 0.000 description 1
- 206010021143 Hypoxia Diseases 0.000 description 1
- 239000007760 Iscove's Modified Dulbecco's Medium Substances 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 208000001738 Nervous System Trauma Diseases 0.000 description 1
- 240000005373 Panax quinquefolius Species 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 229930040373 Paraformaldehyde Natural products 0.000 description 1
- 208000018737 Parkinson disease Diseases 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 101710183548 Pyridoxal 5'-phosphate synthase subunit PdxS Proteins 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 208000006011 Stroke Diseases 0.000 description 1
- 229920004890 Triton X-100 Polymers 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 230000003187 abdominal effect Effects 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 210000000625 blastula Anatomy 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 238000001354 calcination Methods 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 210000003321 cartilage cell Anatomy 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000012930 cell culture fluid Substances 0.000 description 1
- 230000006727 cell loss Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- OEYIOHPDSNJKLS-UHFFFAOYSA-N choline Chemical compound C[N+](C)(C)CCO OEYIOHPDSNJKLS-UHFFFAOYSA-N 0.000 description 1
- 229960001231 choline Drugs 0.000 description 1
- 230000001713 cholinergic effect Effects 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 235000019628 coolness Nutrition 0.000 description 1
- 208000029078 coronary artery disease Diseases 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000005034 decoration Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 230000004064 dysfunction Effects 0.000 description 1
- 230000007831 electrophysiology Effects 0.000 description 1
- 238000002001 electrophysiology Methods 0.000 description 1
- 210000003890 endocrine cell Anatomy 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 229960003692 gamma aminobutyric acid Drugs 0.000 description 1
- BTCSSZJGUNDROE-UHFFFAOYSA-N gamma-aminobutyric acid Chemical compound NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 description 1
- 229930195712 glutamate Natural products 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 210000002266 hair cells auditory Anatomy 0.000 description 1
- 201000010235 heart cancer Diseases 0.000 description 1
- 208000024348 heart neoplasm Diseases 0.000 description 1
- 230000007954 hypoxia Effects 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 210000004263 induced pluripotent stem cell Anatomy 0.000 description 1
- 229910017053 inorganic salt Inorganic materials 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 210000002901 mesenchymal stem cell Anatomy 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 210000002894 multi-fate stem cell Anatomy 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 210000000663 muscle cell Anatomy 0.000 description 1
- 210000000107 myocyte Anatomy 0.000 description 1
- 210000000653 nervous system Anatomy 0.000 description 1
- 208000028412 nervous system injury Diseases 0.000 description 1
- 210000005155 neural progenitor cell Anatomy 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- 229920002866 paraformaldehyde Polymers 0.000 description 1
- 230000008506 pathogenesis Effects 0.000 description 1
- 231100000915 pathological change Toxicity 0.000 description 1
- 230000036285 pathological change Effects 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 210000004927 skin cell Anatomy 0.000 description 1
- 210000004336 spermatogonium Anatomy 0.000 description 1
- 238000009168 stem cell therapy Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 210000002268 wool Anatomy 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0618—Cells of the nervous system
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/20—Transition metals
- C12N2500/24—Iron; Fe chelators; Transferrin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/44—Thiols, e.g. mercaptoethanol
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/33—Insulin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/08—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from cells of the nervous system
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
- C12N2506/1307—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from adult fibroblasts
Landscapes
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Neurosurgery (AREA)
- Neurology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims (8)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610952074.4A CN106350490B (zh) | 2016-11-02 | 2016-11-02 | 利用无血清培养基从成纤维细胞中获得神经细胞的方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610952074.4A CN106350490B (zh) | 2016-11-02 | 2016-11-02 | 利用无血清培养基从成纤维细胞中获得神经细胞的方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN106350490A CN106350490A (zh) | 2017-01-25 |
CN106350490B true CN106350490B (zh) | 2019-10-11 |
Family
ID=57865102
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201610952074.4A Active CN106350490B (zh) | 2016-11-02 | 2016-11-02 | 利用无血清培养基从成纤维细胞中获得神经细胞的方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN106350490B (zh) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107151656A (zh) * | 2017-05-10 | 2017-09-12 | 中国科学院广州生物医药与健康研究院 | 一种提高体细胞向神经细胞转分化效率的方法 |
CN116396928A (zh) * | 2023-05-12 | 2023-07-07 | 细新(上海)医疗科技有限公司 | 一种原代成纤维细胞的培养体系、培养方法和应用 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102533653A (zh) * | 2011-12-15 | 2012-07-04 | 中国人民解放军第二军医大学 | MSCs、ASCs诱导的5-HT神经细胞及其微囊化制备方法与应用 |
CN105087481A (zh) * | 2015-08-21 | 2015-11-25 | 深圳爱生再生医学科技有限公司 | 无血清培养基及干细胞的培养方法 |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20090086260A (ko) * | 2006-12-07 | 2009-08-11 | 테바 파마슈티컬 인더스트리즈 리미티드 | 미손상 골수 또는 미손상 제대 조직으로부터 조직 전구체 세포 및 성숙 조직 세포를 형성 및 증식시키는 방법 |
-
2016
- 2016-11-02 CN CN201610952074.4A patent/CN106350490B/zh active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102533653A (zh) * | 2011-12-15 | 2012-07-04 | 中国人民解放军第二军医大学 | MSCs、ASCs诱导的5-HT神经细胞及其微囊化制备方法与应用 |
CN105087481A (zh) * | 2015-08-21 | 2015-11-25 | 深圳爱生再生医学科技有限公司 | 无血清培养基及干细胞的培养方法 |
Non-Patent Citations (3)
Title |
---|
Adult human olfactory neural progenitors cultured in defined medium.;Xiaodong Zhang et al.;《Experimental neurology》;20040204;第186卷(第2期);第112-123页 * |
Patient fibroblasts-derived induced neurons demonstrate autonomous neuronal defects in adult-onset Krabbe disease;Su Min Lim et al.;《Oncotarget》;20161021;第7卷(第46期);第74507页第1栏第3段 * |
VEGF转染对MSCs向神经细胞诱导分化的影响;杨丽红;《中华神经外科疾病研究杂志》;20100831;第9卷(第4期);第322-324页 * |
Also Published As
Publication number | Publication date |
---|---|
CN106350490A (zh) | 2017-01-25 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US9115342B2 (en) | Method for purifying cardiomyocytes or programmed cardiomyocytes derived from stem cells or fetuses | |
CN102191221B (zh) | 一种能无限自我更新的神经干细胞、其制备方法及其用途 | |
Quattrocelli et al. | Mouse and human mesoangioblasts: isolation and characterization from adult skeletal muscles | |
CN110484506B (zh) | 胶质母细胞瘤类器官模型的构建方法和应用 | |
CN103667349B (zh) | 一种高效获取猪诱导性多能干细胞的方法 | |
CN109486751A (zh) | 用于无饲养细胞培养牛和猪的精原干细胞的方法 | |
CN105861428B (zh) | 一种诱导成纤维细胞转分化为心肌细胞的诱导培养基及其应用 | |
WO2017097007A1 (zh) | 分化培养基及其在制备神经干细胞中的用途 | |
Yang et al. | A simple and efficient method for deriving neurospheres from bone marrow stromal cells | |
CN106047800B (zh) | 猪多能干细胞定向诱导分化为雄性生殖细胞的方法及专用培养基 | |
CN102161980B (zh) | 用人间充质干细胞为滋养层培养诱导多能干细胞的方法 | |
CN101984050B (zh) | 用于产生诱导多能干细胞(iPS)的细胞类型及其制备方法和应用 | |
Wiley et al. | Generation of xeno‐free, cGMP‐compliant patient‐specific iPSCs from skin biopsy | |
CN106350490B (zh) | 利用无血清培养基从成纤维细胞中获得神经细胞的方法 | |
CN106282113A (zh) | 一种利用无血清培养基通过转分化获得神经细胞的方法 | |
CN111344392B (zh) | 一种细胞诱导的方法 | |
CN1884494B (zh) | 诱导人胚胎干细胞向肝脏细胞分化的方法及其专用培养基 | |
KR102146274B1 (ko) | 장관 오가노이드의 제조 방법 및 이의 용도 | |
CN114807034A (zh) | 一种人多能干细胞来源的Müller细胞的制备方法 | |
CN103074297B (zh) | 家畜精原干细胞培养液 | |
Chen et al. | Trophic factor induction of human umbilical cord blood cells in vitro and in vivo | |
CN101613717A (zh) | 用猪成纤维细胞生成诱导的多能性干细胞的方法 | |
CN110093305A (zh) | 一种诱导肝细胞体外扩增的方法 | |
CN103031274A (zh) | 小分子化合物tws119作为神经干细胞分化诱导剂的新应用 | |
CN106282097A (zh) | 诱导多能干细胞、制备诱导多能干细胞的方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right |
Effective date of registration: 20240227 Address after: Room 104, Building 2, No. 198 Kaiyuan Avenue, Huangpu District, Guangzhou City, Guangdong Province, 510700 Patentee after: Zhongke Meisi Stem Cell Regenerative Medical Technology (Guangzhou) Co.,Ltd. Country or region after: China Address before: 510530 open source Avenue 190, Science City, Luogang District, Guangzhou, Guangdong. Patentee before: GUANGZHOU INSTITUTES OF BIOMEDICINE AND HEALTH, CHINESE ACADEMY OF SCIENCES Country or region before: China |
|
TR01 | Transfer of patent right |